CN116035116A - Preparation method and application of enzyme-containing seaweed powder - Google Patents
Preparation method and application of enzyme-containing seaweed powder Download PDFInfo
- Publication number
- CN116035116A CN116035116A CN202210626777.3A CN202210626777A CN116035116A CN 116035116 A CN116035116 A CN 116035116A CN 202210626777 A CN202210626777 A CN 202210626777A CN 116035116 A CN116035116 A CN 116035116A
- Authority
- CN
- China
- Prior art keywords
- seaweed
- enzyme
- powder
- mixing
- seaweed powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001474374 Blennius Species 0.000 title claims abstract description 111
- 239000000843 powder Substances 0.000 title claims abstract description 78
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 61
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 61
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 229940088598 enzyme Drugs 0.000 claims abstract description 60
- 238000002156 mixing Methods 0.000 claims abstract description 44
- 235000012054 meals Nutrition 0.000 claims abstract description 17
- 239000000783 alginic acid Substances 0.000 claims abstract description 14
- 229920000615 alginic acid Polymers 0.000 claims abstract description 14
- 229960001126 alginic acid Drugs 0.000 claims abstract description 14
- 235000010443 alginic acid Nutrition 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 12
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 10
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 10
- 240000008042 Zea mays Species 0.000 claims abstract description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 10
- 235000005822 corn Nutrition 0.000 claims abstract description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 9
- 239000008103 glucose Substances 0.000 claims abstract description 9
- -1 pectase Proteins 0.000 claims abstract description 9
- 238000005406 washing Methods 0.000 claims abstract description 9
- 108010059892 Cellulase Proteins 0.000 claims abstract description 8
- 102000004317 Lyases Human genes 0.000 claims abstract description 8
- 108090000856 Lyases Proteins 0.000 claims abstract description 8
- 108091005804 Peptidases Proteins 0.000 claims abstract description 8
- 239000004365 Protease Substances 0.000 claims abstract description 8
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 8
- 229940106157 cellulase Drugs 0.000 claims abstract description 8
- 238000007873 sieving Methods 0.000 claims abstract description 8
- 239000002245 particle Substances 0.000 claims abstract description 5
- 239000004576 sand Substances 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 20
- 241000195493 Cryptophyta Species 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 16
- 241000512259 Ascophyllum nodosum Species 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 11
- 230000004151 fermentation Effects 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 241000227647 Fucus vesiculosus Species 0.000 claims description 7
- 238000001694 spray drying Methods 0.000 claims description 7
- 241000196253 Ulva prolifera Species 0.000 claims description 6
- 150000004781 alginic acids Chemical class 0.000 claims description 5
- 241000195474 Sargassum Species 0.000 claims description 4
- 241000264279 Sargassum fusiforme Species 0.000 claims description 4
- 241001261506 Undaria pinnatifida Species 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 4
- 241001282806 Sargassum natans Species 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 241000015177 Saccharina japonica Species 0.000 claims description 2
- 238000010564 aerobic fermentation Methods 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 claims description 2
- 230000035484 reaction time Effects 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 230000007281 self degradation Effects 0.000 claims 1
- 238000002386 leaching Methods 0.000 abstract description 7
- 239000004480 active ingredient Substances 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 3
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 238000004925 denaturation Methods 0.000 abstract description 2
- 230000036425 denaturation Effects 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000000413 hydrolysate Substances 0.000 description 13
- 230000002255 enzymatic effect Effects 0.000 description 12
- 240000002234 Allium sativum Species 0.000 description 10
- 239000003337 fertilizer Substances 0.000 description 10
- 235000004611 garlic Nutrition 0.000 description 10
- 239000007788 liquid Substances 0.000 description 9
- 239000003674 animal food additive Substances 0.000 description 8
- 239000006228 supernatant Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- 241000233866 Fungi Species 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 5
- 230000000593 degrading effect Effects 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000003895 organic fertilizer Substances 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000004720 fertilization Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000557833 Hua gabonii Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 241000199919 Phaeophyceae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000037208 balanced nutrition Effects 0.000 description 1
- 235000019046 balanced nutrition Nutrition 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000005580 one pot reaction Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Organic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a preparation method and application of enzyme-containing seaweed powder, comprising the following steps: washing large seaweed, removing sand, dehydrating, sun-drying, crushing, sieving with a 60-100 mesh sieve to obtain seaweed powder with uniform particle size, mixing cellulase, pectase, protease and alginic acid lyase according to the ratio of 5-10:3-6:1-2:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to the ratio of 300-1000: 1 to obtain seaweed meal. The beneficial effects are as follows: the seaweed powder is prepared from the raw materials of seaweed powder, corn stalks, bean pulp, bran, glucose and the like, wherein the seaweed powder is filtered by a 60-100-mesh sieve to prepare fine powder, so that the leaching rate is improved, the extraction time is shortened, the degradation, denaturation, damage and the like of active ingredients caused by long-time high-temperature conditions can be effectively reduced, and natural active ingredients in seaweed can be reserved to the greatest extent.
Description
Technical Field
The invention relates to the technical field of seaweed powder, in particular to a preparation method and application of enzyme-containing seaweed powder.
Background
The sea area of China is wide, the natural condition is superior, the wild seaweed resources in each sea area are rich, but the large brown seaweed is relatively less, and the raw materials required by fertilizer and feed mainly depend on imported seaweed. At present, chemical fertilizers, pesticides and the like are used for improving the yield of crops and reducing the occurrence of epidemic diseases in the planting industry, and antibiotics are mainly used for treating various diseases in the feed industry. The adverse consequences of the accompanying pesticide residue exceeding standard, environmental pollution and the like are more serious, and even endanger human health. With the improvement of the living standard of people, the requirements on the quality and nutrition of crops and livestock products are also higher.
In the prior art, the seaweed has extremely rich and balanced nutrition, has strong adsorption capacity, and can concentrate marine substances which are equivalent to 44 ten thousand times of the seaweed. Polysaccharide, polyphenols, alkaloids, mannitol, vitamins, algae-derived hormone, nitrogen, phosphorus, potassium macroelements, iron, boron, molybdenum, iodine and other microelements in the seaweed not only have good promotion effect on plant growth, but also can promote the growth of aquatic organisms.
However, because of the strong adsorption capacity of seaweed, the heavy metals contained in seaweed are subject to exceeding standards, and in addition, most of the nutrients in seaweed are insoluble and difficult to degrade, so that the seaweed is not beneficial to absorption of plants and animals.
Disclosure of Invention
The invention aims to provide a preparation method and application of enzyme-containing seaweed powder, which are used for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: a preparation method of enzyme-containing seaweed powder comprises the following steps:
the preparation method of the enzyme-containing seaweed powder is characterized by comprising the following steps:
washing large seaweed, removing sand, dehydrating, sun-drying, crushing, sieving with a 60-100 mesh sieve to obtain seaweed powder with uniform particle size, mixing cellulase, pectase, protease and alginic acid lyase according to the ratio of 5-10:3-6:1-2:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to the ratio of 300-1000: 1, mixing to obtain seaweed meal;
taking 40-60 parts of corn stalks, 30-50 parts of bean pulp, 20-40 parts of bran and 5-15 parts of glucose (each component is calculated according to parts by weight), sun-drying, crushing, mixing uniformly, adding 500-700 parts of water, mixing uniformly, sealing, sterilizing, inoculating bacillus subtilis, fermenting for 48 hours to obtain fermentation liquor, and spray-drying the fermentation liquor to obtain an enzyme and bacterium mixture;
mixing the seaweed meal with enzyme and bacteria according to the proportion of 50-100: 1 to obtain the finished product of the enzyme-containing seaweed powder.
Preferably, the coarse powder of seaweed obtained by washing large seaweed has a particle size of 60 mesh or less, and the mixture is dried to a moisture content of 10% or less.
Preferably, the enzyme and bacteria mixture is produced by aerobic fermentation, and the bacteria can be selected from agricultural special bacteria or feed special bacteria according to the application of the seaweed powder.
Preferably, the enzyme-containing kelp powder is stored at a temperature below 25 ℃ and under a drying condition, and the self-degradability shelf life is longer than 6 months.
Preferably, the seaweed is Ascophyllum nodosum [Ascophyllum Nodosum]Undaria pinnatifida [Undaria Pinnatifida (Harvey) Suringar]Fucus vesiculosus [Fucus Vesiculosus]Enteromorpha prolifera [Enteromorpha prolifera (Muller) J. Agardh]Sargassum [ sic ]Sargassum Natans]Sargassum fusiforme [Sargassumfusiforme (Harvey) Setch]Or/and kelp [Laminaria japonica Areschoug]Etc.
When the enzyme-containing seaweed powder is used, the seaweed powder is mixed with hot water at about 40 ℃ and is intermittently stirred, and self-enzymolysis is carried out to obtain an enzymolysis product.
Preferably, the enzymolysis condition is 40-50 ℃, the reaction time is 12-36 hours, the water-insoluble alginic acid in the seaweed is converted into water-soluble small molecule alginic acid without basically viscosity, and the dissolution rate of the alginic acid is more than 90%; the kelp fibers degrade into softened fibers.
Compared with the prior art, the invention has the beneficial effects that:
the preparation method and the application of the enzyme-containing seaweed powder provided by the invention are prepared by adopting seaweed powder, corn straw, bean pulp, bran, glucose and the like as raw materials, wherein the seaweed powder is filtered by a 60-100-mesh sieve to prepare fine powder, so that the leaching rate is improved, the extraction time is shortened, the degradation, denaturation, damage and the like of active ingredients caused by long-time high-temperature conditions can be effectively reduced, and natural active ingredients in seaweed can be retained to the greatest extent;
the biological macromolecules can be degraded into biological micromolecules which are favorable for absorption through the fermentation of enzymes and bacteria, so that the animals and plants can absorb nutrition conveniently, the yield is improved, and the water absorption, fertilizer absorption and water retention capacity of crops are enhanced;
corn stalks, bean pulp, bran and the like have high nutritive value, contain various natural auxins, vitamins, root mineral trace elements, various essential amino acids and the like, and after fermentation treatment, biological macromolecules are changed into small molecules, so that the water solubility is increased, and the absorption of animals and plants is facilitated;
meanwhile, the compounded beneficial strain can maintain good ecological balance and improve stress resistance of animals, plants, disease resistance and the like; the whole process has no waste residue and waste material discharge, and changes plant straw and seaweed residues into valuable materials.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The invention provides a technical scheme that: a preparation method and application of enzyme-containing seaweed powder comprise the following steps:
washing off impurities on the surface of seaweed, sun-drying until the water content is lower than 10% (mass percent), crushing to obtain seaweed powder, sieving with a 60-mesh sieve to obtain seaweed fine powder, mixing cellulase, pectase, protease and alginic acid lyase according to the ratio of 5:3:1:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to the ratio of 1000:1, mixing to obtain seaweed meal;
taking 40 parts of corn stalk, 30 parts of bean pulp and 20 parts of bran, crushing, mixing uniformly, adding 5 parts of glucose, mixing uniformly, inoculating seaweed degrading bacteria, fermenting for 48 hours, stopping fermentation when the microscopic spore rate is more than 90%, and spray drying to obtain an enzyme and bacteria mixture; the components are calculated according to parts by weight;
mixing the seaweed meal with enzyme and fungus mixture according to the weight ratio of 100:1, adding warm water at 35-45 ℃ to prepare a solid-liquid ratio of 10:1, leaching for 12h;
standing and solid-liquid separating the algae residue enzymatic hydrolysate to obtain enzymatic supernatant and algae residue, wherein the enzymatic supernatant is a water-soluble hydrolysate and can be used as a water-soluble algae organic fertilizer or feed additive, and the algae residue is an insoluble hydrolysate and can be used as a base fertilizer or feed additive;
wherein the seaweed used in this example is kelpLaminaria japonica Areschoug]Purchased from kelp farmers in Qingdao.
Example two
The invention provides a technical scheme that: a preparation method and application of enzyme-containing seaweed powder comprise the following steps:
washing off impurities on the surface of seaweed, sun-drying until the water content is lower than 10% (mass percent), crushing to obtain seaweed powder, sieving with an 80-mesh sieve to obtain seaweed fine powder, mixing cellulase, pectase, protease and alginic acid lyase according to the ratio of 7:3:2:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to the ratio of 500:1, mixing to obtain seaweed meal;
taking 50 parts of corn stalk, 40 parts of bean pulp and 30 parts of bran, crushing, mixing uniformly, adding 10 parts of glucose, mixing uniformly, inoculating seaweed degrading bacteria, fermenting for 48 hours, stopping fermentation when the microscopic spore rate is more than 90%, and spray drying to obtain an enzyme and bacteria mixture; the components are calculated according to parts by weight;
mixing the seaweed meal with enzyme and fungus mixture according to the weight ratio of 80:1, adding warm water at 35-45 ℃ to prepare a solid-liquid ratio of 10:1, leaching for 18h;
standing and solid-liquid separating the algae residue enzymatic hydrolysate to obtain enzymatic supernatant and algae residue, wherein the enzymatic supernatant is a water-soluble hydrolysate and can be used as a water-soluble algae organic fertilizer or feed additive, and the algae residue is an insoluble hydrolysate and can be used as a base fertilizer or feed additive;
the seaweed used in this example is Sargassum [ Sargassum ] [Sargassum Natans]Imported from indonesia.
Example III
The invention provides a technical scheme that: a preparation method and application of enzyme-containing seaweed powder comprise the following steps:
washing off impurities on the surface of seaweed, sun-drying until the water content is lower than 10% (mass percent), crushing to obtain seaweed powder, sieving with a 100-mesh sieve to obtain seaweed fine powder, mixing cellulase, pectase, protease and alginic acid lyase according to the ratio of 10:5:2:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to the ratio of 300:1, mixing to obtain seaweed meal;
taking 60 parts of corn stalk, 50 parts of bean pulp and 40 parts of bran, crushing, mixing uniformly, adding 15 parts of glucose, mixing uniformly, inoculating seaweed degrading bacteria, fermenting for 48 hours, stopping fermentation when the microscopic spore rate is more than 90%, and spray drying to obtain an enzyme and bacteria mixture; the components are calculated according to parts by weight;
mixing the seaweed meal with enzyme and fungus mixture according to the weight ratio of 50:1, adding warm water at 35-45 ℃ to prepare a solid-liquid ratio of 10:1, leaching for 36h;
standing and solid-liquid separating the algae residue enzymatic hydrolysate to obtain enzymatic supernatant and algae residue, wherein the enzymatic supernatant is a water-soluble hydrolysate and can be used as a water-soluble algae organic fertilizer or feed additive, and the algae residue is an insoluble hydrolysate and can be used as a base fertilizer or feed additive;
the seaweed used in this example is Ascophyllum nodosum [Ascophyllum Nodosum]Imported from Chilean.
Example IV
The invention provides a technical scheme that: a preparation method and application of enzyme-containing seaweed powder comprise the following steps:
washing off impurities on the surface of seaweed, sun-drying until the water content is lower than 10% (mass percent), crushing to obtain seaweed powder, sieving with a 100-mesh sieve to obtain seaweed fine powder, mixing cellulase, pectase, protease and alginic acid lyase according to the ratio of 10:4:1:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to the ratio of 300:1, mixing to obtain seaweed meal;
taking 60 parts of corn stalk, 50 parts of bean pulp and 40 parts of bran, crushing, mixing uniformly, adding 15 parts of glucose, mixing uniformly, inoculating seaweed degrading bacteria, fermenting for 48 hours, stopping fermentation when the microscopic spore rate is more than 90%, and spray drying to obtain an enzyme and bacteria mixture; the components are calculated according to parts by weight;
mixing the seaweed meal with enzyme and fungus mixture according to the weight ratio of 100:1, adding warm water at 35-45 ℃ to prepare a solid-liquid ratio of 7:1, leaching for 20h;
standing and solid-liquid separating the algae residue enzymatic hydrolysate to obtain enzymatic supernatant and algae residue, wherein the enzymatic supernatant is a water-soluble hydrolysate and can be used as a water-soluble algae organic fertilizer or feed additive, and the algae residue is an insoluble hydrolysate and can be used as a base fertilizer or feed additive;
the seaweed used in this example is Fucus vesiculosus [ Fucus vesiculosus ]Fucus Vesiculosus]Imported from Chilean.
Example five
The invention provides a technical scheme that: a preparation method and application of enzyme-containing seaweed powder comprise the following steps:
washing off impurities on the surface of seaweed, sun-drying until the water content is lower than 10% (mass percent), crushing to obtain seaweed powder, sieving with a 100-mesh sieve to obtain seaweed fine powder, mixing cellulase, pectase, protease and alginic acid lyase according to a ratio of 8:4:2:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to a ratio of 600:1, mixing to obtain seaweed meal;
taking 60 parts of corn stalk, 40 parts of bean pulp and 20 parts of bran, crushing, mixing uniformly, adding 15 parts of glucose, mixing uniformly, inoculating seaweed degrading bacteria, fermenting for 48 hours, stopping fermentation when the microscopic spore rate is more than 90%, and spray drying to obtain an enzyme and bacteria mixture; the components are calculated according to parts by weight;
mixing the seaweed meal with enzyme and fungus mixture according to the weight ratio of 100:1, adding warm water at 35-45 ℃ to prepare a solid-liquid ratio of 7:1, leaching for 18h;
the seaweed used in this example is Enteromorpha prolifera [Enteromorpha prolifera (Muller) J. Agardh]Collecting floating seaweed on the sea surface of the smoke table, the Wired sea and the Qingdao.
Example six
The invention provides a technical scheme that: an application of enzyme-containing seaweed powder:
the experimental place is in a laboratory, 20 pots are planted, and 6 garlic sprouts are planted in each pot, wherein 4 pots of a control group are planted in total, 24 garlic sprouts are planted in each pot, and 16 pots of an experimental group are planted in each pot (4 pots of an experimental group 1, an experimental group 2, an experimental group 3 and an experimental group 4). 10 g of the seaweed insoluble hydrolysate prepared in example 1 was applied per pot in the experimental group, and 10 g of the common organic granular fertilizer was applied per mu in the control group. The fertilization time is as follows: before planting. Diluting the seaweed water-soluble enzymolysis product 10 times every 10 days, irrigating an experimental group, and irrigating a control group with common water-soluble fertilizer, wherein the water-soluble fertilizer in each basin is 80 milliliters.
Results: 40-50 days after fertilization, the garlic plants of experimental groups 1, 2 and 3 have developed root systems, more fibrous roots, long roots, strong stems and increased leaf areas; the garlic plants in the control group have better root system development, but have fewer fibrous roots than those in the experimental group, the stems are thinner than those in the experimental group, and the length of the leaves is shortened.
On the 70 th day after fertilization, the experimental group grows well and no soil-borne disease is found; in the control group, one pot shows mould, so that plants are yellowing and growth is inhibited.
Harvesting: the average fresh weight of the garlic sprouts of the experimental group 1 is 13.1586g, the average fresh weight of the garlic sprouts of the experimental group 2 is 12.9753g, the average fresh weight of the garlic sprouts of the experimental group 3 is 13.1437g, the average fresh weight of the garlic sprouts of the experimental group 4 is 12.9962g, and the average fresh weight of the garlic sprouts of the control group is 10.8557g. The average fresh weight of the garlic sprouts of the experimental group is 13.0685g, the average fresh weight of the garlic sprouts of the control group is 10.8557g, and compared with the control group, the experimental group can increase the yield by 20.4 percent.
Conclusion: the enzyme-containing seaweed powder can obviously improve the yield of garlic sprouts and can effectively prevent and treat soil-borne diseases.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The preparation method of the enzyme-containing seaweed powder is characterized by comprising the following steps:
washing large seaweed, removing sand, dehydrating, sun-drying, crushing, sieving with a 60-100 mesh sieve to obtain seaweed powder with uniform particle size, mixing cellulase, pectase, protease and alginic acid lyase according to the ratio of 5-10:3-6:1-2:1 to obtain mixed enzyme powder, and mixing the seaweed powder and the mixed enzyme powder according to the ratio of 300-1000: 1, mixing to obtain seaweed meal;
taking 40-60 parts of corn stalks, 30-50 parts of bean pulp, 20-40 parts of bran and 5-15 parts of glucose (each component is calculated according to parts by weight), sun-drying, crushing, mixing uniformly, adding 500-700 parts of water, mixing uniformly, sealing, sterilizing, inoculating bacillus subtilis, fermenting for 48 hours to obtain fermentation liquor, and spray-drying the fermentation liquor to obtain an enzyme and bacterium mixture;
mixing the seaweed meal with enzyme and bacteria according to the proportion of 50-100: 1 to obtain the finished product of the enzyme-containing seaweed powder.
2. The method for preparing the enzyme-containing seaweed powder according to claim 1, wherein the method comprises the following steps: the coarse powder of sea algae is fine powder with particle size below 60 mesh, and the mixture is dried to water content below 10%.
3. The method for preparing the enzyme-containing seaweed powder according to claim 1, wherein the method comprises the following steps: the enzyme and bacteria mixture is produced by aerobic fermentation, and the strain can be selected from agricultural special strain or feed special strain according to the application of the seaweed powder.
4. The method for preparing the enzyme-containing seaweed powder according to claim 1, wherein the method comprises the following steps: the enzyme-containing kelp powder is stored under the condition of below 25 ℃ and drying, and the self-degradation quality guarantee period is more than 6 months.
5. The method for preparing the enzyme-containing seaweed powder according to claim 1, wherein the method comprises the following steps: the seaweed is Ascophyllum nodosum [ PaulliniaAscophyllum Nodosum]Undaria pinnatifida [Undaria Pinnatifida (Harvey) Suringar]Fucus vesiculosus [Fucus Vesiculosus]Enteromorpha prolifera [Enteromorpha prolifera (Muller) J. Agardh]Sargassum [ sic ]Sargassum Natans]Sargassum fusiforme [Sargassumfusiforme (Harvey) Setch]Or/and kelp [Laminaria japonica Areschoug]Etc.
6. Use of the enzyme-containing seaweed meal as claimed in any of the preceding claims 1 to 5, characterized in that: when the enzyme-containing seaweed powder is used, the seaweed powder is mixed with hot water at about 40 ℃ and is intermittently stirred, and self-enzymolysis is carried out to obtain an enzymolysis product.
7. The use of an enzyme-containing seaweed meal as claimed in claim 6, characterized in that: the enzymolysis condition is 40-50 ℃, the reaction time is 12-36 hours, the water-insoluble alginic acid in the seaweed is converted into water-soluble small molecular alginic acid without basically viscosity, and the dissolution rate of the alginic acid is more than 90%; the kelp fibers degrade into softened fibers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210626777.3A CN116035116A (en) | 2022-06-05 | 2022-06-05 | Preparation method and application of enzyme-containing seaweed powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210626777.3A CN116035116A (en) | 2022-06-05 | 2022-06-05 | Preparation method and application of enzyme-containing seaweed powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116035116A true CN116035116A (en) | 2023-05-02 |
Family
ID=86122624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210626777.3A Pending CN116035116A (en) | 2022-06-05 | 2022-06-05 | Preparation method and application of enzyme-containing seaweed powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116035116A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103549130A (en) * | 2013-10-15 | 2014-02-05 | 内蒙古民族大学 | Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation |
| CN104146150A (en) * | 2014-08-26 | 2014-11-19 | 威海市世代海洋生物科技有限公司 | Seaweed biological feed and preparation method thereof |
| WO2016029646A1 (en) * | 2014-08-26 | 2016-03-03 | 威海市世代海洋生物科技有限公司 | Composite microorganism enzyme, method for preparing plant nutrient solution by using composite microorganism enzyme, and fertilizer synergist |
| CN106974137A (en) * | 2017-05-10 | 2017-07-25 | 合肥华创现代农业科技有限公司 | A kind of penaeus vannamei boone feed |
| CN108185222A (en) * | 2018-01-31 | 2018-06-22 | 青岛海兴源生物科技有限公司 | A kind of aquatic feeds with adhesive effect use seawood meal |
| CN108935933A (en) * | 2018-08-02 | 2018-12-07 | 合肥河多宝农业开发有限公司 | Prawn mixed feed and preparation method thereof |
| CN109485473A (en) * | 2018-12-29 | 2019-03-19 | 青岛海大生物集团有限公司 | A kind of dedicated seaweed microbial manure of rice in saline-alkali field |
| CN110235992A (en) * | 2019-06-14 | 2019-09-17 | 珠海市斗门区基壮农业发展有限公司 | A kind of feed containing seaweed |
| CN111348977A (en) * | 2020-04-23 | 2020-06-30 | 青岛海力源生物科技有限公司 | Bacterial enzyme linked alginic acid fertilizer synergist and preparation method thereof |
-
2022
- 2022-06-05 CN CN202210626777.3A patent/CN116035116A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103549130A (en) * | 2013-10-15 | 2014-02-05 | 内蒙古民族大学 | Method for producing seaweed protein feed through synergistic effect of enzymolysis and fermentation |
| CN104146150A (en) * | 2014-08-26 | 2014-11-19 | 威海市世代海洋生物科技有限公司 | Seaweed biological feed and preparation method thereof |
| WO2016029646A1 (en) * | 2014-08-26 | 2016-03-03 | 威海市世代海洋生物科技有限公司 | Composite microorganism enzyme, method for preparing plant nutrient solution by using composite microorganism enzyme, and fertilizer synergist |
| CN106974137A (en) * | 2017-05-10 | 2017-07-25 | 合肥华创现代农业科技有限公司 | A kind of penaeus vannamei boone feed |
| CN108185222A (en) * | 2018-01-31 | 2018-06-22 | 青岛海兴源生物科技有限公司 | A kind of aquatic feeds with adhesive effect use seawood meal |
| CN108935933A (en) * | 2018-08-02 | 2018-12-07 | 合肥河多宝农业开发有限公司 | Prawn mixed feed and preparation method thereof |
| CN109485473A (en) * | 2018-12-29 | 2019-03-19 | 青岛海大生物集团有限公司 | A kind of dedicated seaweed microbial manure of rice in saline-alkali field |
| CN110235992A (en) * | 2019-06-14 | 2019-09-17 | 珠海市斗门区基壮农业发展有限公司 | A kind of feed containing seaweed |
| CN111348977A (en) * | 2020-04-23 | 2020-06-30 | 青岛海力源生物科技有限公司 | Bacterial enzyme linked alginic acid fertilizer synergist and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 岳明;丁宏标;乔宇;: "海藻酸裂解酶酶学与基因工程研究进展及应用", 生物技术通报, no. 06, 26 December 2006 (2006-12-26), pages 5 - 8 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102515952B (en) | Chitosan coated alga bioorganic fertilizer and preparation method thereof | |
| CN104817408B (en) | A kind of high-performance bio bacterial manure and preparation method thereof | |
| CN105110894A (en) | Multi-functional biological organic bacterial fertilizer and production method therefor | |
| CN101468923B (en) | Bio-waste disposal method and apparatus | |
| CN107254420B (en) | Bacillus subtilis and application thereof | |
| CN105237147A (en) | Culture medium for oyster mushroom and preparation method thereof | |
| CN102503727A (en) | High-activity traditional Chinese medicine organic bacterial manure and manufacturing method | |
| CN102491839A (en) | Method for manufacturing multienzyme-complex organic fertilizer by using Chinese herbal medicine | |
| CN112159776A (en) | Complex enzyme preparation for improving fertility of cultivated land and preparation method thereof | |
| CN103265354B (en) | Bio-organic fertilizer prepared from modeling clay slag as main material and preparation method thereof | |
| CN113812327B (en) | Vegetable seedling substrate based on waste utilization and preparation method thereof | |
| CN111302855A (en) | Oligosaccharide organic peptide fertilizer and preparation method thereof | |
| CN107660443A (en) | Semiarid region apple grunt ox hickory chick circular agriculture system construction method | |
| CN105110829A (en) | Mushroom culture substrate and preparation method thereof | |
| CN110981635A (en) | Agricultural microbial agent with nitrogen fixation, phosphorus dissolution, potassium dissolution and cellulose dissolution functions and preparation method thereof | |
| CN102503606B (en) | Biological fertilizer special for organic peanuts and preparation method thereof | |
| CN111675555A (en) | Application of anabaena and/or spirulina extract as biostimulant in agricultural production | |
| CN110981638A (en) | Biological organic fertilizer with saline-alkali soil improvement effect and preparation process thereof | |
| CN109503228A (en) | A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk | |
| CN1217883C (en) | Pure organic fertilizer | |
| CN116035116A (en) | Preparation method and application of enzyme-containing seaweed powder | |
| CN115572203A (en) | Preparation method of subtropical fruit tree seaweed microbial fertilizer | |
| CN108675866A (en) | Special water-soluble microbial-bacterial fertilizer of a kind of legume crop and preparation method thereof | |
| CN113072412A (en) | Disease-preventing fertilizer for fruit trees and preparation method thereof | |
| CN113200795A (en) | Enzymolysis biological peptide fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |