CN1160334A - Method of cultivating mushroom or like, and storage bag for mushroom culture container or like - Google Patents
Method of cultivating mushroom or like, and storage bag for mushroom culture container or like Download PDFInfo
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- CN1160334A CN1160334A CN95195653A CN95195653A CN1160334A CN 1160334 A CN1160334 A CN 1160334A CN 95195653 A CN95195653 A CN 95195653A CN 95195653 A CN95195653 A CN 95195653A CN 1160334 A CN1160334 A CN 1160334A
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- nonwoven
- medium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
- A01G18/66—Cultivation bags
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Abstract
A method of cultivating mushroom, in which a culture medium feed port and an additional ventilation window of a container are sealed with a nonwoven fabric having a thickness of 0.10 to 2.00 mm, an apparent density of 0.05 to 0.50 g/cm<3> and a gas permeability of 10 to 500 cc/m<2>/sec when spawns or mycelium is cultivated in a container such as a bag. Further, the culture container is contained in a storage bag of a nonwoven fabric for cultivation whereby breeding of various bacteria in an initial stage is effectively prevented in a simple operation without the use of a sterilized room.
Description
The present invention relates to the artificial culture method of Mushrooms (I ェ Class) and the employed gas permeability seal of container of interior dress medium.
Medicinal Mushrooms such as edible Mushrooms such as mushroom, beautiful gill fungus, plain gill fungus, fagus gill fungus, flat mushroom, dance mushroom (white compound leaf shape in face is brown), mushroom and glossy ganoderma can be cultivated through people's work post gill fungus, mycelium is cultivated and the cultivation of gill fungus and grow and gather in the crops.
The cultivation initial stage, middle plant gill fungus, mycelium can be planted on the medium of suitable selection, suitably manage the moisture, temperature, air of the medium in the culture vessel etc., prevent that black mould, wood are mould, to invade the gill fungus medium be very important to harmful mushroom of head mold etc.
The mycelium cultural method of above-mentioned edible gill fungus, as the Japanese patent gazette spy opens clear 58-28212 number announcement, earlier mycelial medium is filled into synthetic resin for example in the bag container of polyethylene, polypropylene etc., with topped firmly top filling mouths of bag such as degerming filter clothes, after sterilization or the sterilization, take off degerming filter cloth etc., again kind of a gill fungus is inoculated on the medium, topped again degerming filter cloth is put into culturing room to bag and is cultivated.
In cultivation, the ventilative of medium undertaken by the degerming filter cloth.Open clear 62-11030 number, spy the Japanese patent gazette spy and open flat 3-61477 number, spy and open in flat 5-328844 number and disclosed a kind of culture vessel, the position of this culture vessel beyond its filling oral area is provided with pore or is provided with the louver(-vre) of making of paper, nonwoven etc.
During container was cultivated, the most normal problem that runs into was the assorted bacterium breeding problem of medium in the container.Assorted spreading of bacterium causes gill fungus not grow.
Should assorted bacterium spread over mushroom mycelium grow into contain before the gesture, be that initial stage especially becomes problem, under common or poor environment, cause producing the substandard products about 3～15%.When the culture medium inoculated kind gill fungus, though be in the transfer room of having made corresponding tube reason, to carry out, or in the culturing room that has made corresponding tube reason, to carry out, in fact above-mentioned two Room all are not desinfection chamber completely, still have a considerable amount of assorted bacterium swimming indoor.
Assorted bacterium in the container cultivation spreads breeding problem, how is caused by the installation of the degerming filter cloth of the bag filling mouth of bag container.
Topped on the filling mouth time degerming filter cloth etc., for example adopt degerming filter cloth made of paper, rigid plastics system cylindrical body and above penetrate the rigid plastics lid occasion of several apertures, the periphery of bag peristome (inlet portion) is passed cylindrical body, this periphery is folded to the outside from the inboard of cylindrical body, with the topped firmly peristome of degerming filter cloth, again lid is withheld the periphery that is entrenched in cylindrical body, the bag periphery and the degerming filter cloth periphery of turnover are held under the arm between lid and cylindrical body.
Above-mentioned filling on the cylindrical body and periphery of the synthetic resin bag body between the lid and degerming filter cloth periphery of the container of medium and bag, must form many wrinkle, these wrinkle make the little gap of the about 0.1～1.0mm of formation between both sides' periphery.Its result, though sealed up the degerming filter cloth at the filling mouth (inlet) of bag container, but still can not prevent that assorted bacterium is from these gaps intrusion medium.Therefore, the degerming filter cloth in the bag container method topped only just can avoid assorted bacterium to invade in transfer room under gnotobasis and the culturing room, and therefore, its expense is very high.
Problem of the present invention is, at the container cultivation of edible Mushrooms, particularly adopt in the Mushrooms cultivation of bag container class, reduces assorted bacterium and invades from the medium filling oral area and the transom of bag container.
Another problem of the present invention is, in the mycelium bag container cultivation of mushroom, provides a kind of cost-effective device especially, can prevent the living contaminants in the medium with easy method.
The purpose of this invention is to provide a kind of easy method, in the artificial culture method of using the Mushrooms culture vessel, in the overall process of the cultivation that comprises kind of gill fungus, mycelial cultivation, can economically, significantly reduce assorted bacterium and invade in the medium.
According to the present invention, above-mentioned problem is that following cultural method solves, that is, in the container of Mushrooms was cultivated, the ventilative of gill fungus medium was to be undertaken by specific nonwoven cloth material basically, and the average apparent density of this specific nonwoven is 0.05～0.5g/cm
3, gas permeability is 10～500c.c./cm
2/ sec.
The gas permeability of the nonwoven that the present invention is used is 10～500cc/cm
2/ sec is preferably 30～300cc/cm
2/ sec.As the not enough 10cc/cm of gas permeability
2During/sec, be difficult to guarantee to be suitable for the environmental condition that mycelium is cultivated, hinder mycelial growth.When surpassing 500cc/cm
2During/sec, be difficult to prevent to be subjected to the pollution of assorted bacterium on every side.Gas permeability is the value of measuring with Fu Lajier (the Off ラ ジ ュ-Le) testing machine of JIS-L-1096.The average apparent density of the used nonwoven of the present invention is 0.05～0.5g/cm
3, be preferably 0.1～0.3g/cm
3As the not enough 0.05g/cm of average apparent density
3The time, the space is big, and strainability is poor, is difficult to prevent living contaminants.When surpassing 0.5g/cm
3The time, though strainability is good, gas permeability, softness, nobility are poor, and be wrinkling easily and make the chimeric not tight of cylindrical body and lid.
Here said nonwoven can be with single method such as spun-bonded fabric method (spunbond), melt-spun spinning method (meltblow), a roller comb silk method, dry type short fiber method or method combination and being made into more than two kinds.The used fiber of nonwoven can be the synthetic fibers of polyolefin, polyamide, polyester, polyacrylonitrile etc., perhaps native fiber such as cotton, and the diameter of fiber is 0.5m～100 μ, is preferably 1～80 μ.When diameter less than 0.5 μ of fiber, gas permeability is poor, and when surpassing 100 μ, strainability is poor.
For the gas permeability and the strainability that make this nonwoven is good, fibrous material, the different fiber of fibre diameter can be mixed and knit or lamination, again endowing antibacterial etc.
For example, can be to be fibre diameter the nonwoven that the superfine fibre nonwoven of 0.5～6 μ and non-woven fabrics of synthetic fiber lamination that fibre diameter is 10～100 μ obtain, and to nonwoven endowing antibacterial of above-mentioned formation filter cloth etc.For example, can mix nonwoven in the synthetic resin to the silver-colored class inorganic antiseptic of silver ion and phosphoric acid salt, zeolite etc., perhaps pay method on the fiber the 4th grade of ammonium salt, organic nitrogen compound, organosilicon etc.
The weight per unit area that forms the nonwoven of this filter cloth is 20～200g/cm
3, be preferably 40～150g/cm
3
In the cultivation of the present invention, medium ventilative has following two kinds of methods in the container.(1) the peristome envelope is ended method, that is, the medium filling mouth or the paying of container of sealing culture vessel (preferably bag container) with above-mentioned specific nonwoven add ventilation mouth.(2) the storage bag envelope is ended method,, culture vessel is put into again the harvesting bag made from nonwoven that is.The storage bag envelope method of ending of the vessel port of above-mentioned (1) envelope being ended method and (2) combines, and can prevent spreading of assorted bacterium more effectively.
(1) the peristome envelope is ended method
Adopting the opening of container envelope to end the cultural method of method, is to be 0.10～2.0mm, average apparent density 0.05～0.5g/cm with thickness
3, gas permeability is 10～500cc/cm
2The nonwoven of/sec topped and seal the medium filling mouth of container or additionally be located on the container, with ventilative (saturating wet) window of medium filling mouth split.
Promptly, utilize following edible gill fungus cultural method, can prevent the breeding of assorted bacterium rather easily, the method is characterized in that: kind gill fungus of gill fungus or mycelial medium are filled in the culture vessel, after medium carried out sterilization treatment, inoculation kind of gill fungus or mycelium are 0.10～2.0mm, average apparent density 0.05～0.5g/cm with thickness
3, gas permeability is 10～500cc/cm
2/ minute nonwoven topped and seal the peristome of container.
Adopting the peristome envelope to end the better embodiment of the cultivation of the present invention of method, is the method that envelope is ended the filling mouth (inlet portion) of container class.Envelope only is performed such, promptly, entangle the periphery of the medium filling mouth of bag container class with rigid column, when being installed in lid on bag filling mouth periphery and the cylindrical body, hold under the arm nonwoven in the little gap between cylindrical body and lid and fix, nonwoven can be sealed the medium filling mouth of container.In order to make nonwoven be fixed on the cylindrical body periphery, preferably adopt nonwoven that do not crease easily, that have softness and nobility with the identical ground of cylindrical body shape, the unoccupied place of not leaving a blank.The thickness of nonwoven is 0.10～2.0mm, is preferably 0.20～1.50mm.During thickness is not enough 0.10mm, can not fully seal the little gap between cylindrical body and the lid, when thickness surpasses 2.00mm, not cylindrical body and lid chimeric not tight.
Be installed in the lid of container or the nonwoven in the little gap between cylindrical body on the bag and the lid, will have softness especially.For example, its hard and soft degree should be preferably 5cm～13cm below 15cm.This value is the hard and soft degree of measuring with 45 ° of cantilever methods of JIS-L1906, and when surpassing 15cm, the chimeric meeting of cylindrical body and lid is not tight because of little gaps of generation such as wrinkle.
Shape, the material of container are not particularly limited, as long as can keep being suitable for the medium of gill fungus growth.Its material can adopt polyolefin, polyamide, polyester of polyethylene, polypropylene etc. etc.The satchel that bag can adopt the transparent or semitransparent film of systems such as polyethylene system, polypropylene to make.In addition, can on the inlet portion of bag, install rigid plastics system cylindrical body and above penetrate the hard cover of several holes, this nonwoven perhaps is installed on the part of the top of bag.About shape, can be circular, square, rectangle etc., its size and shape all are not particularly limited, but will keep bag ventilative good to oxygen, carbonic acid gas etc.
As mentioned above, the shape of nonwoven of the present invention, size etc. are different because of the difference of culture vessel and bag, adopt in the method for cylindrical body and lid in aftermentioned, preferably adopt the square nonwoven of 9～10cm.
This cultural method can prevent to inoculate the living contaminants behind kind of the gill fungus.
In order effectively to prevent living contaminants, fully carry out the sterilization of medium.Behind sterilization process, the inoculation of refrigerating work procedure, kind gill fungus etc. will be noted and must carry out under aseptic environments.For example, in the outlet of pasteurization device, the simple and easy confined chamber of the nonwoven with gas permeability, resistance to water, antibiotic property etc. is set, in this indoor inoculation of carrying out kind of gill fungus.
(2) the storage bag envelope is ended method
The culture vessel class is left in method in the nonwoven bag, is that mycelium culture vessel or the bag of gill fungus are put into storage bag, and at least a portion of this storage bag is that to adopt average apparent density be 0.05～0.5g/cm
3, gas permeability is 10～500cc/cm
2The nonwoven of/sec is made.
When leaving in culture vessel in this storage bag,, be used in the booth or indoor cultivation just like gnotobasis.So-called gnotobasis, its original meaning are meant aseptic indoor environment or carried out the environment etc. of spray disinfection with commercially available bactericide such as halogen, peroxide, alcohol type.But in the present invention, owing to adopt storage bag, in cultivation, stage especially in the early stage, can prevent the living contaminants that causes because of little gap, so, even when cultivating, do not adopt the desinfection chamber of high price, also can realize very high production operation.In doors, basement, grottoes, and raintight booth, tent in etc. can cultivate.
Storage bag of the present invention can prevent living contaminants preferably and keep gas permeability preferably.When the gas permeability difference, can not keep the required air capacity of mycelial growth, and under high temperature environment such as summer, be not easy to regulate the temperature of medium.Therefore, the nonwoven that constitutes this storage bag preferably adopts the nonwoven of the present invention that gas permeability is strong, have the intransitable gap of assorted bacterium, thickness.
Can be placed on culture vessel or bag respectively singly in the storage bag of the present invention, perhaps a plurality of cultivations are placed on what nonwoven and air impermeability material were made with container or bag and indoorly carry out the mycelial cultivation of gill fungus.Deposit the existing cultivation of adopting the degerming filter cloth also can be prevented assorted bacterium with container or bag effect with storage bag of the present invention.
In addition, the storage bag envelope is ended method, seals the fragrance of the gill fungus of being cultivated because of using storage bag, can obtain having the edible gill fungus of approximate log cultivation fragrance.
Pack in this storage bag above-mentioned cultivation with container or bag after, be placed on shelf etc. and go up or hanging, can cultivate, be the cultural method of operation, excellent in operability.
This storage bag adopts nonwoven of the present invention, or a part adopts the lucite film of polyolefin described later, polyamide, polyester etc.In order to improve cementability, adopt the laminated film of the different pellicular cascade of heat-sealing processing or melting point.Adhering method can be that hot press, high frequency engage or bonding agent engages.
After having put into said vesse or bag, seal this storage bag peristome again.Its encapsulating method is that the peristome of storage bag periphery is sealed up with for example heat bonding or high frequency sealing, bonding agent etc.Also can fold up the periphery of this peristome, tie this folding part with belt or rubber band etc., or it is fixing with staple, paper clip etc., for example use near the not top of the accommodation bag peristome of bent aluminum, copper pole or plate object or helix, after 3～4 circles are folding, this folding part is bent to the inside promptly salable.
The storage bag that nonwoven of the present invention is made can prevent living contaminants well, good growing environment is provided for the mycelium of activate.
Therefore,, pack medium doubly, can achieve the above object effectively cultivating initial stage.
In addition, in gill fungus medium cultivation, gather in the crops an after date,, can also gather in the crops second phase, three phases again if manage the amount of moisture of gill fungus medium and living contaminants etc. preferably.
For example, the gill fungus medium after the results is directly put into the storage bag made from nonwoven of the present invention, managed amount of moisture (prevent too drying, keep the environment of suitable humidity) and living contaminants, can make mycelium propagation, gill fungus is born once more.
Fig. 1 is the key diagram that the present invention cultivates the embodiment of edible mushroom method, in this method, seals with the inlet portion that the bag of mycelium medium is housed in the nonwoven handle, and the vertical section structure of the bag of medium is housed in the expression among the figure.
Fig. 2 represents the cultivation bag that medium is housed in shown in Figure 1 is put into the method that storage bag of the present invention is cultivated edible mushroom again, its vertical section of expression among the figure.
Fig. 3 is that mycelium of the present invention is cultivated side view (A) and the stereogram (B) with bag.
Fig. 4 is that mycelium of the present invention is cultivated side view (A), stereogram (B) and the ground plan (C) with bag.
Fig. 5 is the side view (A) and the stereogram (B) of storage bag of the present invention.
The following describes embodiments of the invention.
Fig. 1, Fig. 2, Fig. 3 and Fig. 4 represent to seal with specific nonwoven the embodiment of the invention of culture vessel peristome, cultivation Mushrooms.
Because the part of container adopts above-mentioned specific nonwoven, so this container has the required gas permeability and the aseptic of bacterium from the outside intrusion that prevent to mix of mycelium of cultivating gill fungus.The mode of sealing vessel port with nonwoven has two kinds, and a kind of is that filling medium in the container and the used filling oral area of inoculation bacterial classification are sealed, another kind inlet portion in addition, the transom that is attached on the part of container seals.
Fig. 1 represents the cultural method the bag inlet portion sealed with specific nonwoven of the present invention.Among Fig. 1, the 1st, bag passes the inboard of cylindrical body 3 with its head, is folded to the cylindrical body outside again.The 4th, lid is provided with the hole 5 of ventilative usefulness at its top.Nonwoven 2 of the present invention is held under the arm between container part that is folded to cylindrical body 3 outsides and lid 4, and container is airtight.Nonwoven 2 has aseptic and gas permeability, can supply with sufficient air and keep the aseptic of medium to medium 6.
Fig. 3 and Fig. 4 inlet portion that to be expression seal container or bag with nonwoven of the present invention is the mode of part opening in addition, and bag 20,30 is made of the body part 22,32 and the nonwoven part of the present invention 21,31 of bag.In this case, the part of peristome 21,31 must no longer contact with medium behind the filling medium.
For example, at the tight lower position place of bag peristome, it is 5～50cm that area is set
2Shaped aperture such as circle, Long Circle, square, rectangle, these openings can be provided with a place or number place separately, with fixed bags that firmly just obtains having gas permeability such as bonding agent, adhesive, heat-sealings.
Fig. 2 represents bag shown in Figure 1 is put into again the state of aftermentioned storage bag 10.
The medium of 1.0～2.5kg in cultivation with common filling in container or the bag, has certain weight, topped firmly this culture vessel or bag with above-mentioned storage bag, of the bottom surface bending of the peristome periphery down of this storage bag, utilize above-mentioned weight just can seal effectively again towards culture vessel or bag.
Storage bag shape of the present invention is done some improvement, also can observe cultivation conditions at any time.
Below, cultural method of the present invention is described.Cultivate the mycelial medium of gill fungus, can adopt common medium.For example can adopt the nutritional blend of rice bran, wheat bran, corn flour, sawdust and commodity and the water of 65% weight to mix.
Specify cultural method of the present invention below.Fig. 1 represents one of typical cultural method example.Medium 6 is filled in the bag 1, cylindrical body 3 be installed in bag peristome 1 ' on, along the top circumference of cylindrical body 3, the square nonwoven 2 of topped for example 9cm at the center above lid 4 being entrenched in, carries out sterilization process, refrigerating work procedure then again.In order to stop the assorted bacterium that returns in air or the cooling after the sterilization to be invaded, when holding under the arm this nonwoven 2 between cylindrical body 3 and lid 4, had better not allow nonwoven 2 wrinkling.
Then, under gnotobasis, take off be installed in bag 1 peristome 1 ' on lid 4 and nonwoven 2, after planting gill fungus and being inoculated on the medium 6, topped again nonwoven 2 of the present invention, chimeric upper cover 4 is airtight, cultivates.In addition, also can hold nonwoven between opening of container and the lid under the arm, compress to connect airtight and similarly cultivate with above-mentioned bag.
When using Fig. 3 and bag shown in Figure 4, also can similarly cultivate.
Storage bag of the present invention can prevent living contaminants better and keep big as far as possible gas permeability.If gas permeability is poor, then can not keeps the required air capacity of mycelial growth, and under high temperature environment such as summer, be not easy to regulate the temperature of medium.Therefore, the nonwoven that constitutes this storage bag preferably adopts the nonwoven of the present invention that gas permeability is strong, have intransitable gap of assorted bacterium and thickness.
Storage bag of the present invention can use specific nonwoven of the present invention in all or part of ground, for example, as shown in Figure 5, can be ventilative portion with nonwoven 41 of the present invention, airtight the satchel made from transparent or semitransparent film of synthetic resin 42.The shape of storage bag of the present invention does not have special restriction, cultivates with container or bag as long as can pack into, but preferably as shown in Figure 5, adopts the lucite film at airtight, can observe internal state like this.In this case, the observation part preferably is located at shady place or covers with nonwoven of the present invention, so that be not subjected to periods of direct sunlight in the time in addition of observing.Storage bag of the present invention preferably has at least the part more than 50% to adopt nonwoven of the present invention.
Be placed on respectively in the storage bag of the present invention singly with container or bag cultivating, or a plurality of cultivations with container or bag be placed on nonwoven and gas impermeable material make indoor, all can cultivate the gill fungus mycelium.In addition, the cultivation of adopting existing degerming filter cloth is put into the effect that harvesting facility of the present invention also can obtain antiforeign bacteria with container or bag.
After above-mentioned cultivation put into this storage bag with container or bag, resting shelf etc. and go up or hanging and can cultivate, is the cultural method of operation, good operability.
This storage bag is made with nonwoven of the present invention, or as illustrated in fig. 5, its part adopts the lucite film of polyolefin, polyamide, polyester etc.In order to improve cementability, the laminated film that adopts heat sealability and the different film stack of melting point to be set to.Adhering method can be thermo-compressed sealing, high frequency sealing or bonding agent sealing.
After having put into said vesse or bag, seal the peristome of this storage bag again.Its encapsulating method is that the peristome of storage bag periphery is sealed up with for example heat bonding or high frequency sealing, bonding agent etc.Also can fold up the periphery of this peristome, tie this folding part with belt or rubber band etc., or it is fixing with staple, paper clip etc., for example use near the not top of the accommodation bag peristome of bent aluminum, copper pole or plate object or helix, after 3～4 circles are folding, this folding part is bent to the inside promptly salable.
Fig. 2 represents bag shown in Figure 1 is put into again the state of storage bag 10.Common medium 1.0～2.5kg in filling in cultivating with container or bag, has certain weight, topped firmly this culture vessel or bag with above-mentioned storage bag, of the bottom surface bending of the downwardly facing opening portion periphery of this storage bag, utilize above-mentioned weight just can realize sealing effectively towards culture vessel or bag.
Storage bag shape of the present invention is done some improvement, can observe cultivation conditions at any time.
Embodiment
With embodiment the present invention is described below, but the scope of the invention is not limited to these embodiment.
The determination method of using among the present invention is as follows.
Thickness: (JIS-L-1096), to 5 different positions of test portion, with thickness tester load 100g/cm within a certain period of time
2Down, the thickness average value that records.
Average apparent density: gravimetry and thickness, obtain the weight of per unit volume.
Gas permeability: be the value of measuring with (JIS-L1096) Fu Lajier (Off ラ ジ ユ-Le) testing machine.
Synthetic resin bag body: the polypropylene bag body that capacity is 2 liters (wide 140mm, high 330mm, fold into the flat box-like of 50mm)
Medium: by 5 parts in rice bran (weight ratio, down together), 30 parts in wheat bran, 10 parts of corn flour, 65 parts in sawdust, water mix for 65 parts, altogether 1.2kg (diameter 150mm, the cylindric block of high 130mm)
The sterilization of medium and cooling: put after 5 hours and be chilled to 20 ℃ with normal pressure steam (95 ℃ of the temperature) sterilization of circulating.
Plant the kind of gill fungus: mushroom
Cylindrical body and lid: rigid plastics system
The size of cylindrical body (internal diameter 46mm, external diameter 49mm)
(top offers 10 to the size of lid for internal diameter 50mm, external diameter 53mm
The aperture of 3mm diameter)
Condition of culture: 22 ℃ ± 2 ℃ of temperature, humidity 50～60%.
Cultivate fate: 15 days (the aseptic management of no cold air and air-supply etc.)
Nonwoven is to adopt its Si Nilong (ェ Le ス Na ィ ロ Application) (trade name, Asahi Chemical Industry Co., Ltd's system, thickness 0.55mm, average apparent density 0.21g/cm of nonwoven fabric of long fibers Ai Lu
3, gas permeability 106cc/cm
2/ sec), it is square to make 9cm, prepares 100.Fig. 1 represents to cultivate the skiagraph with bag, and this cultivation is the nonwoven 2 of 10.5cm with having adopted hard and soft degree on the bag.
With " its this polypropylene of nonwoven fabric of long fibers Ai Lu (ェ Le ス Port リ プ ロ ピ レ Application) " (trade name, Asahi Chemical Industry Co., Ltd's system, thickness 0.50mm, average apparent density 0.14g/cm
3, gas permeability 145cc/cm
2/ sec), hard and soft degree 9.6cm) and polypropylene film making storage bag shown in Figure 5.Make the storage bag of 100 wide 30cm, high 40cm, its film section is the transparent airtight part of wide 10cm, high 40cm, contrast with bag with the cultivation of embodiment 1, with 25～30 ℃ temperature, 30～80% humidity, cultivated 30 days in the tent without, remaining condition is identical with embodiment 1.After the cultivation, check that the result of medium is the whole well-growns of the mycelium of the mushroom on medium.
Except being 60 days between culture period, to cultivate altogether beyond 50, all the other are identical with embodiment 2.
Nonwoven held under the arm between cylindrical body and lid, carry out chimericly, reduced little gap, improved gas permeability, the cultivation results of checking 60 days is that first phase is cultivated and finished in 25 days, the activate phenomenon occurs after 20 days again, be medium top protuberance, beginning brown stain, all initial stage of origination brown stains during 60 days.
Adopt nonwoven of the present invention to make the bag of adorning medium, and the storage bag of depositing this bag, the water evaporates that activate is produced, carbonic acid gas discharge etc., ventilative good, can carry out first phase cultivation, the second stage of cultivation well.
Reference examples 1
Prepare the square commercially available degerming filter cloth of 100 9cm (brown paper of Japanese papermaking commercial firm system, thickness 0.10mm, average apparent density 0.6g/cm
3, gas permeability is 1.2cc/cm
2/ sec).
Nonwoven of the present invention with among the above-mentioned brown paper replacement embodiment 1 carries out cultivation similarly to Example 1.
The degerming filter cloth of hard brown paper, its softness, nobility are poor, and gas permeability is little, when chimeric, produces wrinkle so hold under the arm between cylindrical body and lid, and seal is poor, can not prevent fully that assorted bacterium from invading.After the cultivation, check that the result of medium is, have the bacterium of mixing on 73 place's medium to spread.
Reference examples 2
Prepare the square commodity degerming filter cloth of 100 9cm rich (デ ュ Port Application) commercial firm's system it with visit ( ィ ベ Star Network) (registration mark), thickness 0.17mm, average apparent density 0.35g/cm
3, gas permeability is 1.0cc/cm
2/ sec).
Nonwoven of the present invention with above-mentioned degerming filter cloth (polyethylene nonwoven) replacement embodiment 1 carries out cultivation similarly to Example 1.
Because softness, nobility are poor, to hold under the arm between cylindrical body and lid and when chimeric, produce wrinkle, seal is poor, can not prevent fully that assorted bacterium from invading.After the cultivation, check that the result of medium is, have the assorted bacterium of 47 place's medium to spread.
Reference examples 3
Except cultivate 60 days, with 50 cultivations, all the other are identical with reference examples 1.
Check that the result who cultivates is, assorted bacterium spreads in 35 medium, and other medium was finished first phase with 30～35 days and cultivated, after 25～30 days, the beginning activate is compared with using nonwoven of the present invention again, assorted bacterium is invaded easily, and activation phenomenon postpones 5～10 days.
Embodiment 4
The bag that adopts commercially available degerming filter cloth (reference examples 1) is put into the storage bag of embodiment 2, carry out cultivation similarly to Example 2.
After the cultivation, check that the result of medium is, the mycelium of mushroom whole well-growns and be advantage in medium can prevent living contaminants preferably.
Except cultivating fate is 60 days, and cultivating number is beyond 50, and all the other are identical with embodiment 2.
The cultivation results of checking 60 days is, finishes first phase in 32 days to cultivate, and beginning activation phenomenon again after 25 days is medium top protuberance, the beginning brown stain.In 60 days cultivation, not the brown stain that all reaches the initial stage.
The bag that adopts commercially available degerming filter cloth (reference examples 2) is put into the storage bag of embodiment 2, carry out cultivation similarly to Example 2.
After the cultivation, check that the result of medium is, whole well-growns of the mycelium of mushroom and be advantage can prevent living contaminants preferably.
Polypropylene film is made bag shown in Figure 3 (high 45cm, wide 20cm, the invagination degree of depth is 6cm), the 15cm position middle body down from bag inlet portion upper end, be provided with the hole of diameter 30mm, the nonwoven fabric of long fibers polypropylene of wide 6cm, long 7cm (ェ Le ス Port リ プ ロ ピ レ Application) (Asahi Chemical Industry Co., Ltd's system, thickness 0.46mm, average apparent density 0.13g/cm
3, gas permeability is 165cc/cm
2/ sec, hard and soft degree are 8.5cm) be that the center is bonding with heat-resistant glue with the 30mm diametric hole of this bag, make the cultivation bag.Cultivate mushroom with method similarly to Example 1.During sterilization, seal inlet portion, make its cooling after the sterilization with adhesive tape.After planting the gill fungus inoculation, clamp the bag inlet portion with the aluminium sheet of long 18cm, wide 1cm, thick 0.8mm, this aluminium sheet is folded seal inlet portion, all the other are identical with the method for embodiment 1.After the cultivation, check that the result of medium is, whole well-growns of the mycelium of the mushroom in medium and be advantage.
Embodiment 8
The cultivation of embodiment 7 is put into the storage bag of embodiment 2 with bag, carry out the cultural method of embodiment 2.
After the cultivation, check that the result of medium is, whole well-growns of the mycelium of the mushroom in medium and be advantage.
The feature of Mushrooms cultural method of the present invention is, adopting specific nonwoven of the present invention to seal gill fungus cultivates with opening of container or uses the storage bag of specific nonwoven, cultivate the mycelium of gill fungus, especially can prevent the living contaminants of initial stage, the required air capacity of mycelial growth of gill fungus can be provided, water evaporates, carbonic acid gas that activate is produced are discharged, and desinfection chamber that can high price can be cultivated edible gill fungus very easy, economically.With method of the present invention can be in roof, booth and tent, carry out aseptic culture under the indoor environment.In addition, culture vessel is put into the storage bag cultured method again, can obtain having the edible gill fungus of approximate log cultivation fragrance.
Claims (6)
1. eat the cultural method of gill fungus, it is characterized in that, bacterial classification of gill fungus or mycelial medium are filled in the culture vessel, above-mentioned medium is carried out sterilization treatment after, inoculation bacterial classification or mycelium are that 0.10～2.0mm, average apparent density are 0.05～0.50g/cm with thickness
3, gas permeability is 10～500cc/cm
2/ minute nonwoven peristome topped and that seal culture vessel cultivate.
2. edible gill fungus cultural method as claimed in claim 1 is characterized in that container is a bag, by nonwoven topped peristome be the medium filling mouth of bag.
3. as claim 1 and 2 described edible gill fungus cultural methods, it is characterized in that the hard and soft degree of nonwoven is below the 15cm, this hard and soft degree is the value of measuring with 45 ° of cantilever methods of JIS-L-1906.
4. depositing the harvesting bag of edible gill fungus culture vessel, it is characterized in that, is to be 0.05～0.50g/cm with average apparent density
3, gas permeability is 10～500cc/cm
2/ minute nonwoven make.
5. eat the cultural method of gill fungus, it is characterized in that, bacterial classification of gill fungus or mycelial medium are filled in the culture vessel, above-mentioned medium is carried out sterilization treatment after, inoculation bacterial classification or mycelium are sealed the inlet of culture vessel and are cultivated with the thin-film material of gas permeability.
6. eat the cultural method of gill fungus, it is characterized in that, bacterial classification of gill fungus or mycelial medium are filled in the culture vessel, above-mentioned medium is carried out sterilization treatment after, inoculation bacterial classification or mycelium are that 0.10～2.0mm, average apparent density are 0.05～0.50g/cm with thickness
3, gas permeability is 10～500cc/cm
2/ minute nonwoven topped and seal the peristome of culture vessel after, packing into average apparent density is 0.05～0.50g/cm
3, gas permeability is 10～500cc/cm
2/ minute the harvesting bag made of nonwoven in cultivate.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24956294 | 1994-10-14 | ||
| JP249562/96 | 1994-10-14 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1160334A true CN1160334A (en) | 1997-09-24 |
| CN1094304C CN1094304C (en) | 2002-11-20 |
Family
ID=17194852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95195653A Expired - Fee Related CN1094304C (en) | 1994-10-14 | 1995-10-13 | Method of cultivating mushroom or like, and storage bag for mushroom culture container or like |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN1094304C (en) |
| WO (1) | WO1996011565A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
| CN109511459A (en) * | 2018-10-18 | 2019-03-26 | 河北中尔新材料有限公司 | Packet mushroom piece and preparation method thereof |
| CN109769592A (en) * | 2019-03-27 | 2019-05-21 | 儋州牧春绿色生态农业开发有限公司 | A kind of cultural method of ganoderma lucidum |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6230932B2 (en) * | 2014-02-21 | 2017-11-15 | 株式会社サカト産業 | Bag for grape cultivation |
| JP2017118847A (en) * | 2015-12-28 | 2017-07-06 | キリン株式会社 | Mass production method of plants, and mass production equipment, and culture bags used therefor |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5828212A (en) * | 1981-08-07 | 1983-02-19 | 株式会社九州化学 | Constitution of culture medium in mushroom culturing |
| JPS6135721A (en) * | 1984-07-30 | 1986-02-20 | 有限会社コンペツクス | Mushroom culture method |
| JPS6135722A (en) * | 1984-07-30 | 1986-02-20 | 有限会社 コンペツクス | Artificial culture log and mushroom culture method |
| JPS6211030A (en) * | 1985-07-05 | 1987-01-20 | 森産業株式会社 | Mushroom culture bag |
| CN1107283A (en) * | 1993-07-08 | 1995-08-30 | 旭化成工业株式会社 | Storage material and method used for culturing mushroom filament |
-
1995
- 1995-10-13 CN CN95195653A patent/CN1094304C/en not_active Expired - Fee Related
- 1995-10-13 WO PCT/JP1995/002112 patent/WO1996011565A1/en active Search and Examination
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105875202A (en) * | 2016-06-13 | 2016-08-24 | 蔡志闯 | Inoculation mechanism, strain containers and method for culturing edible mushrooms |
| CN109511459A (en) * | 2018-10-18 | 2019-03-26 | 河北中尔新材料有限公司 | Packet mushroom piece and preparation method thereof |
| CN109769592A (en) * | 2019-03-27 | 2019-05-21 | 儋州牧春绿色生态农业开发有限公司 | A kind of cultural method of ganoderma lucidum |
| CN109769592B (en) * | 2019-03-27 | 2021-06-25 | 儋州牧春绿色生态农业开发有限公司 | Lucid ganoderma cultivation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1094304C (en) | 2002-11-20 |
| WO1996011565A1 (en) | 1996-04-25 |
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