CN115807109A - 一种基于pcr技术的扇贝品种鉴定方法及其特异性引物 - Google Patents
一种基于pcr技术的扇贝品种鉴定方法及其特异性引物 Download PDFInfo
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- CN115807109A CN115807109A CN202211645920.XA CN202211645920A CN115807109A CN 115807109 A CN115807109 A CN 115807109A CN 202211645920 A CN202211645920 A CN 202211645920A CN 115807109 A CN115807109 A CN 115807109A
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- 235000020637 scallop Nutrition 0.000 title claims abstract description 75
- 241000237509 Patinopecten sp. Species 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 25
- 241001441955 Argopecten irradians Species 0.000 claims abstract description 30
- 241001526627 Azumapecten farreri Species 0.000 claims abstract description 18
- 238000012408 PCR amplification Methods 0.000 claims abstract description 9
- 241000237516 Mizuhopecten yessoensis Species 0.000 claims description 15
- 238000001962 electrophoresis Methods 0.000 claims description 9
- 238000010186 staining Methods 0.000 claims description 3
- 241000237515 Chlamys nipponensis Species 0.000 claims 1
- 230000003321 amplification Effects 0.000 abstract description 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 description 17
- 241000237503 Pectinidae Species 0.000 description 13
- 239000000980 acid dye Substances 0.000 description 6
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- 108020004707 nucleic acids Proteins 0.000 description 6
- 102000039446 nucleic acids Human genes 0.000 description 6
- 241000318639 Argopecten purpuratus Species 0.000 description 5
- 239000011543 agarose gel Substances 0.000 description 4
- 238000007400 DNA extraction Methods 0.000 description 3
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000003147 molecular marker Substances 0.000 description 3
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- 108090000623 proteins and genes Proteins 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 2
- 238000009360 aquaculture Methods 0.000 description 2
- 244000144974 aquaculture Species 0.000 description 2
- 238000001976 enzyme digestion Methods 0.000 description 2
- 241001441956 Argopecten Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 101000920078 Homo sapiens Elongation factor 1-alpha 1 Proteins 0.000 description 1
- 108091092195 Intron Proteins 0.000 description 1
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- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 241000180586 Pterioida Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
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- 230000004048 modification Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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CN202211645920.XA CN115807109A (zh) | 2022-12-20 | 2022-12-20 | 一种基于pcr技术的扇贝品种鉴定方法及其特异性引物 |
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CN202211645920.XA CN115807109A (zh) | 2022-12-20 | 2022-12-20 | 一种基于pcr技术的扇贝品种鉴定方法及其特异性引物 |
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Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2809118A1 (fr) * | 2000-05-16 | 2001-11-23 | Hokkaido Federation Of Fisheri | Procede pour analyser la lignee phyletique des coquilles saint-jacques |
CN103602738A (zh) * | 2013-11-12 | 2014-02-26 | 辽宁大学 | 一种快速鉴定扇贝品种的多重pcr引物及方法 |
CN105256056A (zh) * | 2015-11-17 | 2016-01-20 | 万超 | 一种扇贝物种特异性检测引物及应用 |
CN106498086A (zh) * | 2016-12-30 | 2017-03-15 | 青岛农业大学 | 紫扇贝与海湾扇贝及其回交后代母本来源的鉴定方法 |
CN106701948A (zh) * | 2016-12-30 | 2017-05-24 | 青岛农业大学 | 紫扇贝、海湾扇贝与杂交后代母本来源的鉴定方法 |
CN112176074A (zh) * | 2020-11-04 | 2021-01-05 | 辽宁省海洋水产科学研究院 | 一种检测虾夷扇贝的实时荧光pcr引物探针及方法 |
CN112695105A (zh) * | 2021-02-18 | 2021-04-23 | 辽宁省海洋水产科学研究院 | 栉孔扇贝的实时荧光pcr鉴定方法 |
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- 2022-12-20 CN CN202211645920.XA patent/CN115807109A/zh active Pending
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2809118A1 (fr) * | 2000-05-16 | 2001-11-23 | Hokkaido Federation Of Fisheri | Procede pour analyser la lignee phyletique des coquilles saint-jacques |
US20020081593A1 (en) * | 2000-05-16 | 2002-06-27 | Koji Nagashima | Method for analyzing phyletic lineage of scallop |
CN103602738A (zh) * | 2013-11-12 | 2014-02-26 | 辽宁大学 | 一种快速鉴定扇贝品种的多重pcr引物及方法 |
CN105256056A (zh) * | 2015-11-17 | 2016-01-20 | 万超 | 一种扇贝物种特异性检测引物及应用 |
CN106498086A (zh) * | 2016-12-30 | 2017-03-15 | 青岛农业大学 | 紫扇贝与海湾扇贝及其回交后代母本来源的鉴定方法 |
CN106701948A (zh) * | 2016-12-30 | 2017-05-24 | 青岛农业大学 | 紫扇贝、海湾扇贝与杂交后代母本来源的鉴定方法 |
CN112176074A (zh) * | 2020-11-04 | 2021-01-05 | 辽宁省海洋水产科学研究院 | 一种检测虾夷扇贝的实时荧光pcr引物探针及方法 |
CN112695105A (zh) * | 2021-02-18 | 2021-04-23 | 辽宁省海洋水产科学研究院 | 栉孔扇贝的实时荧光pcr鉴定方法 |
Non-Patent Citations (5)
Title |
---|
JONG-MAN YOON: "Genetic Distances of Scallop (Chlamys farreri) Populations investigated by PCR Procedure", 《DEV REPROD》, vol. 21, no. 4, 31 December 2017 (2017-12-31), pages 435 - 440 * |
OSCAR MAURIZ等: "Selection of reference genes for quantitative RT-PCR studies on the gonad of the bivalve mollusc Pecten maximus L.", 《AQUACULTURE》, vol. 370, 11 December 2012 (2012-12-11), pages 163 * |
TIAN LIU等: "K252a inhibition of sperm viability for efficient crossbreeding of hermaphroditic bivalves", 《AQUACULTURE》, vol. 577, 15 December 2023 (2023-12-15), pages 739930 * |
刘凤巧等: "紫扇贝与海湾扇贝杂交后代的母本亲权鉴定方法", 《水产科学》, vol. 36, no. 5, 21 September 2017 (2017-09-21), pages 563 - 568 * |
潘好远等: "虾夷扇贝Smad基因家族的鉴定及表达分析等", 《中国海洋大学学报》, vol. 51, no. 03, 19 January 2021 (2021-01-19), pages 54 - 64 * |
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