CN115715323A - 一种高兼容性的PCR-free建库和测序方法 - Google Patents
一种高兼容性的PCR-free建库和测序方法 Download PDFInfo
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- CN115715323A CN115715323A CN202080099449.1A CN202080099449A CN115715323A CN 115715323 A CN115715323 A CN 115715323A CN 202080099449 A CN202080099449 A CN 202080099449A CN 115715323 A CN115715323 A CN 115715323A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
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Abstract
本发明公开了一种PCR‑free建库和测序方法。本发明提供了一种PCR‑free高通量测序方法，包括如下步骤：将待测序核酸根据大小进行或不进行片段化处理，得到目的大小DNA片段；末端修复和加A反应；连接含有标签序列的接头；进行单链环化和滚环复制，形成DNA纳米球；上机测序。
Description
PCT国内申请，说明书已公开。
Claims (32)
- PCT国内申请，权利要求书已公开。
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2020/096987 WO2021253372A1 (zh) | 2020-06-19 | 2020-06-19 | 一种高兼容性的PCR-free建库和测序方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115715323A true CN115715323A (zh) | 2023-02-24 |
Family
ID=79268915
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202080099449.1A Pending CN115715323A (zh) | 2020-06-19 | 2020-06-19 | 一种高兼容性的PCR-free建库和测序方法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20230265500A1 (zh) |
| EP (1) | EP4170028A4 (zh) |
| CN (1) | CN115715323A (zh) |
| WO (1) | WO2021253372A1 (zh) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024036445A1 (zh) * | 2022-08-15 | 2024-02-22 | 深圳华大智造科技股份有限公司 | 一种测序文库的制备方法及用于制备测序文库的试剂盒 |
| CN115651974B (zh) * | 2022-11-11 | 2024-03-08 | 纳昂达(南京)生物科技有限公司 | 人工模拟cfDNA标准品及其制备方法与应用 |
| WO2024138729A1 (zh) * | 2022-12-30 | 2024-07-04 | 深圳华大生命科学研究院 | Dna连接缓冲液及其应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400776B (zh) * | 2014-09-12 | 2019-12-31 | 深圳华大智造科技有限公司 | 寡核苷酸接头及其在构建核酸测序单链环状文库中的应用 |
| WO2016118883A1 (en) * | 2015-01-23 | 2016-07-28 | Washington University | Detection of rare sequence variants, methods and compositions therefor |
| CN104962551B (zh) * | 2015-07-15 | 2016-08-24 | 北京中科紫鑫科技有限责任公司 | 一种焦磷酸测序文库的构建方法 |
| US11293033B2 (en) * | 2016-05-18 | 2022-04-05 | Amyris, Inc. | Compositions and methods for genomic integration of nucleic acids into exogenous landing pads |
| CN109689872B (zh) * | 2016-11-21 | 2022-12-23 | 深圳华大智造科技股份有限公司 | 一种dna末端修复与加a的方法 |
| GB2561824B (en) * | 2017-04-19 | 2020-04-01 | Canon Kk | Encoding and decoding of geometry data in a 3D model based on evaluated regularity of the model |
| CN108251504A (zh) * | 2018-01-17 | 2018-07-06 | 翌圣生物科技（上海）有限公司 | 一种超快速构建基因组dna测序文库的方法和试剂盒 |
| WO2019217452A1 (en) * | 2018-05-08 | 2019-11-14 | Mgi Tech Co., Ltd. | Single tube bead-based dna co-barcoding for accurate and cost-effective sequencing, haplotyping, and assembly |
| CN111041026B (zh) * | 2019-12-26 | 2022-03-11 | 北京优迅医学检验实验室有限公司 | 一种高通量测序用核酸接头和文库构建方法 |
-
2020
- 2020-06-19 CN CN202080099449.1A patent/CN115715323A/zh active Pending
- 2020-06-19 EP EP20940612.3A patent/EP4170028A4/en active Pending
- 2020-06-19 WO PCT/CN2020/096987 patent/WO2021253372A1/zh unknown
-
2022
- 2022-12-16 US US18/067,540 patent/US20230265500A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4170028A1 (en) | 2023-04-26 |
| EP4170028A4 (en) | 2023-10-18 |
| US20230265500A1 (en) | 2023-08-24 |
| WO2021253372A1 (zh) | 2021-12-23 |
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