CN115667510A - 核酸的纯化方法 - Google Patents

核酸的纯化方法 Download PDF

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Publication number
CN115667510A
CN115667510A CN202180038183.4A CN202180038183A CN115667510A CN 115667510 A CN115667510 A CN 115667510A CN 202180038183 A CN202180038183 A CN 202180038183A CN 115667510 A CN115667510 A CN 115667510A
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CN
China
Prior art keywords
nucleic acid
solution
anionic adsorbent
extraction
recovery
Prior art date
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Pending
Application number
CN202180038183.4A
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English (en)
Chinese (zh)
Inventor
山口创
内田桂
河野隆昌
高松辰典
木下拓也
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sekisui Chemical Co Ltd
Sekisui Medical Co Ltd
Original Assignee
Sekisui Chemical Co Ltd
Sekisui Medical Co Ltd
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Publication date
Application filed by Sekisui Chemical Co Ltd, Sekisui Medical Co Ltd filed Critical Sekisui Chemical Co Ltd
Publication of CN115667510A publication Critical patent/CN115667510A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN202180038183.4A 2020-05-25 2021-05-19 核酸的纯化方法 Pending CN115667510A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020090405 2020-05-25
JP2020-090405 2020-05-25
PCT/JP2021/019014 WO2021241363A1 (ja) 2020-05-25 2021-05-19 核酸の精製方法

Publications (1)

Publication Number Publication Date
CN115667510A true CN115667510A (zh) 2023-01-31

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ID=78744759

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180038183.4A Pending CN115667510A (zh) 2020-05-25 2021-05-19 核酸的纯化方法

Country Status (5)

Country Link
US (1) US20230183674A1 (https=)
EP (1) EP4159855A4 (https=)
JP (1) JP7723657B2 (https=)
CN (1) CN115667510A (https=)
WO (1) WO2021241363A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121737274A (zh) * 2026-02-28 2026-03-27 杭州倍沃医学科技有限公司 一种提高体外扩增dna产量的方法和应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202104814XA (en) * 2019-01-25 2021-08-30 Sekisui Chemical Co Ltd Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999040098A1 (de) * 1998-02-04 1999-08-12 Merck Patent Gmbh Verfahren zur isolierung und aufreinigung von nucleinsäuren
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
EP1527172B1 (en) 2002-05-17 2008-11-26 Molzym GmbH & Co. KG Method for nucleic acid extraction and nucleic acid purification
KR100657957B1 (ko) * 2005-04-12 2006-12-14 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 및 상기방법에 사용될 수 있는 핵산 분리용 고상 물질
JP4340298B2 (ja) 2007-03-01 2009-10-07 株式会社日立ハイテクノロジーズ 核酸回収方法及び核酸回収装置
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
GB0814570D0 (en) * 2008-08-08 2008-09-17 Diagnostics For The Real World Isolation of nucleic acid
WO2011151428A1 (en) 2010-06-01 2011-12-08 Qiagen Gmbh Method for isolating and/or purifying nucleic acid(s)
CN102146369A (zh) * 2010-12-24 2011-08-10 吉林大学 一种基于配位作用的固相介质提取dna的方法
CN113355321A (zh) 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
US20140087366A1 (en) 2012-09-19 2014-03-27 Beckman Coulter, Inc. USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS
US9416356B2 (en) 2013-03-15 2016-08-16 Abbott Molecular Inc. Compositions and methods for nucleic acid extraction
JP6385201B2 (ja) * 2013-08-31 2018-09-05 栄研化学株式会社 核酸増幅に適した試料の調製方法及び試薬キット
DE102016214909B4 (de) * 2015-08-11 2018-05-17 Stem Arts Projects, Llc Tragbare Nukleinsäure-Extraktionsvorrichtung und Verfahren zu deren Verwendung
US11242518B2 (en) * 2015-09-04 2022-02-08 QIAGEN Sciences, LLP Methods for co-isolation of nucleic acids and proteins
JP2019033715A (ja) 2017-08-21 2019-03-07 Jsr株式会社 核酸抽出方法および核酸抽出キット
SG11202104814XA (en) * 2019-01-25 2021-08-30 Sekisui Chemical Co Ltd Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN121737274A (zh) * 2026-02-28 2026-03-27 杭州倍沃医学科技有限公司 一种提高体外扩增dna产量的方法和应用

Also Published As

Publication number Publication date
WO2021241363A1 (ja) 2021-12-02
EP4159855A1 (en) 2023-04-05
JP7723657B2 (ja) 2025-08-14
EP4159855A4 (en) 2024-07-24
JPWO2021241363A1 (https=) 2021-12-02
US20230183674A1 (en) 2023-06-15

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