CN1156177A - Artificial cultivating method for north Chinese caterpillar fungus with rich strontium - Google Patents
Artificial cultivating method for north Chinese caterpillar fungus with rich strontium Download PDFInfo
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- CN1156177A CN1156177A CN 96100721 CN96100721A CN1156177A CN 1156177 A CN1156177 A CN 1156177A CN 96100721 CN96100721 CN 96100721 CN 96100721 A CN96100721 A CN 96100721A CN 1156177 A CN1156177 A CN 1156177A
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- rich strontium
- strontium
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Abstract
A method for artificial cultivation of strontium-enriched north Chinese caterpillar fungus includes bionic method and medium substitution method, which is suitable to produce strontium-enriched north Chinese caterpillar fungus in batches. Obtained north Chinese caterpillar fungus has high cordycepine content up to 0.3% or more, which is tens times higher than that of natural Chinese caterpillar fungus, resulting in special health-care and medical functions. The method features simple process, short period and low cost.
Description
The present invention relates to a kind of artificial cultivating method for north Chinese caterpillar fungus with rich strontium.
In the existing Cordyceps militaris (L.) Link. cultivation, be to be cultivation matrix with mulberry cocoon chrysalis live body, inoculation Cordyceps militaris (L.) Link. bacterium, grow stroma on the pupal cell or with animal and plant class composition through prescription, sterilization, under gnotobasis, inoculate Coragyceps militaris bacterium, cultivating the stroma of growing on the base, or lacquer adds certain rare elements in the cultivation base, makes the Cordyceps militaris (L.) Link. enrichment, reaches and improves the purpose that keeps healthy with the treatment function.
The inventor is in the patent application of Chinese invention patent 93117344.2, adopt rich selenium material cultivation of selenium-rich Cordyceps militaris (L.) Link., output increases, because the enrichment of selenium, curative effect is heightened, but this Cordyceps militaris (L.) Link. characteristic composition of cordycepin is not improved, and for the Cordyceps militaris (L.) Link. deep processing, manually the economic benefit of extracting cordycepin has been brought influence.
The objective of the invention is to avoid the deficiency of above-mentioned prior art, a kind of artificial cultivating method for north Chinese caterpillar fungus with rich strontium that makes this rare precious material high yield of cordycepin is provided, content of cordycepin is reached between the 0.3-0.4%, surpass more than a times of other kinds Cordyceps militaris (L.) Link. 0.1-0.2%, surpass tens times of natural cordyceps.Thereby, improved the economic worth and the utility value of Cordyceps militaris (L.) Link..For Cordyceps militaris (L.) Link. provides preferred material to the extraction of cordycepin.
The object of the present invention is achieved like this:
Choose or produce rich strontium material as the cultivation base, said rich strontium material is the animal and plant class of growth in natural rich strontium mineral water, underground water, the rich strontium area, or utilizes inorganic strontium as the cultivation based additive.
The present invention compares with prior art has following advantage.
1, basic owing to choosing or produce rich strontium material as cultivation, thus make the rich strontium of Cordyceps militaris (L.) Link., and this organic strontium easily is absorbed by the body, has the special efficacy of anti-various modern difficult and complicated illness.
2, because the enrichment of strontium in Cordyceps militaris (L.) Link. cultivation base makes Cordyceps militaris (L.) Link. in process of growth, catalysis the formation of cordycepin, make cordycepin content apparently higher than other kinds Cordyceps militaris (L.) Link. more than one times.Be higher than more than tens times of natural authentic Cordyceps sinensis, improved the economic worth of Cordyceps militaris (L.) Link..
3, owing to the enrichment of strontium in Cordyceps militaris (L.) Link. cultivation base, output improves 5-15%, cultivates 2-6 ℃ of growth temperature tolerance raising.
4, cultivating method is simple, and promotion and implementation are convenient in operation easily, and are with low cost, and the cycle is short.
Below in conjunction with embodiment the present invention is described in further detail.
Choose or produce rich strontium material as the said rich strontium material of cultivation base, be natural mineral water, underground water, the animal and plant class of growth in the rich strontium district, its content is equal to or greater than 0.25PPM, the mineral water domestic as Inner Mongol Balin Right Banner, the domestic underground water of Jiangsu Province's Tongshan County all can be as the cultivation base of present method, or, make the rich strontium of Cordyceps militaris (L.) Link. with in the inorganic selenium additive adding cultivation base.Figure of description is the rich strontium cultivation of a Cordyceps militaris (L.) Link. process block diagram.
With reference to accompanying drawing, strontium element is enriched in the cultivation base, and the stroma content of strontium that cultivation matrix is grown out is equal to or greater than 0.3PPM, and content of cordycepin is also corresponding to be brought up to more than 0.3%.Thereby improved the value of Cordyceps militaris (L.) Link..
Mother culture media can be done raw material with rich strontium material, and it is as follows to fill a prescription:
1, rich strontium dried silkworm chrysalis meal 150-250 gram
White sugar 20-25 gram
Agar 10-25 gram
Potassium primary phosphate 1-3 gram
Sal epsom 1-2 gram
1000 milliliters of rich strontium mineral water, underground water
PH value 5.5-7
It is produced, and the female kind method of solid is identical with parent edible fungus kind production method, and the packing test tube seals then, and conventional autoclaving 30 minutes is inoculated when cooling off 25 ℃, and 20-25 ℃ of dark culturing covered with the inclined-plane in five days.
2, liquid mother culture media
Rich strontium dried silkworm chrysalis meal 150-250 gram
White sugar 20-25 gram
Agar 0-10 gram
Peptone 0-5 gram
Potassium primary phosphate 1-3 gram
Sal epsom 0-2 gram
1000 milliliters of rich strontium mineral water, underground water
PH value 5.5-7
It is identical with the female kind method of edible fungus liquid that it produces liquid mother culture media method, packing is 150 milliliters then, in 500 milliliters of triangular flasks or carboy, tampon seals, the sterilization of wrapping back, high pressure 0.5-1 hour, inoculation when cooling off 25 ℃ makes bacterial classification ferfas silk increase towards last 25 ℃ and supports after 48-72 hour hand 6 times of every day, each 5 minutes, mass production can get final product after 5 days with shaking table 90-125 rev/min.
Cultivation based raw material prescription:
1, choosing rich strontium material is rich strontium rice or other crop rice, rich strontium dried silkworm chrysalis meal etc.
Prescription: rich strontium rice or millet 300-400 gram
Rich strontium dried silkworm chrysalis meal 20-60 gram
Potassium primary phosphate 1-3 gram
Zinc sulfate 0-3 gram
Rich strontium underground water, mineral water 600-700 milliliter
PH value 7-8
2, utilize the prescription of rich strontium additive:
Rice or millet 300-400 gram
Dried silkworm chrysalis meal 20-60 gram
Water 600-700 milliliter
Potassium primary phosphate 1-3 gram
Zinc sulfate 0-3 gram
Rich strontium additive 1-10 gram
PH value 7-8
Cultivating container adopts resistant to elevated temperatures case, dish, bottle etc., but size variation when making according to the condition of production.
Raw material is packed container inner high voltage sterilization 1-2 into frequently, connects bacterium, aseptic technique when cooling off back 25 ℃.
Inoculation method: solid spawn utilizes wiping face or suspension system, makes the bacterial classification dispersed, and liquid spawn is adopted spray method, consumption, and 60 centimetres of every length, wide 40 centimetres, high 30 centimetres case are no less than 150 milliliters, the consumption changeable.
After inoculation finishes, indoor temperature control 18-25 ℃, best 20-22 ℃, dark culturing, indoor relative humidity 60-70% saw scattered light after 20 days, and the 40-60 days stromas of gathering add water or nutritive medium in right amount and can receive two to three batches, and biological transformation ratio is more than 100%.
3, adopt live body silkworm worm to produce rich strontium Cordyceps militaris (L.) Link. method.
Prescription: toothed oak, silkworm worm 50-100 bar
The bright toothed oak of rich strontium, mulberry leaves 500-5000 gram
Cordyceps militaris (L.) Link. bacterium liquid 50-100 milliliter
Silkworm larva is put into case, dish, feed in the time of taking food rich strontium mulberry leaf or toothed oak leaf, 2-3 sprays the Cordyceps militaris (L.) Link. bacterium during age on the silkworm body, 18-23 ℃ of management, relative humidity control 70-80%, make microbiological contamination silkworm body imbed in the soil or the thing of preserving moisture such as wood chip in, can grow stroma, drying or Preservation Treatment in 20-50 days, packing, sealing.
4, adopt living silkworm chrysalises to produce rich strontium Cordyceps militaris (L.) Link. method.
Prescription: live cocoon chrysalis or mulberry silkworm chrysalis 100-200
Be equal to or greater than the rich strontium spraying fluid of 2PPM 0-100 milliliter
Rich strontium Cordyceps militaris (L.) Link. bacterium liquid 50-200 milliliter
Mulberry silkworm chrysalis or cocoon chrysalis are removed cocoon, and the rich strontium liquid of spray is in pupal cell, after the drying earlier, under sanitary condition, spray into or in pupal cell, inject rich strontium Cordyceps militaris (L.) Link. bacterium liquid with syringe, each pupa injects 0.5-5 milliliter, pack in the container after the sterilization, temperature control 15-20 ℃, controlling moisture 70-85%.Dark management about 20 days, when pupal cell hardens, promptly visible scattered light.Go out grass, the 30-40 days herbs of can gathering.Dry back Preservation Treatment, packing, sealing is preserved.
Claims (7)
1, a kind of artificial cultivating method for north Chinese caterpillar fungus, particularly a kind of artificial cultivating method for north Chinese caterpillar fungus with rich strontium is characterized in that: choose or produce rich strontium material as the cultivation base.
2, artificial cultivating method for north Chinese caterpillar fungus with rich strontium according to claim 1 is characterized in that: said rich strontium material is the animal and plant class of growth in natural rich strontium mineral water, underground water, the rich strontium area, or utilizes inorganic strontium as the cultivation based additive.
3, artificial cultivating method for north Chinese caterpillar fungus with rich strontium according to claim 1 and 2, it is characterized in that: natural mineral water, on the ground its content of strontium of water is equal to or greater than 0.25PPM, as the domestic mineral water of Inner Mongol Balin Right Banner, the domestic underground water of Jiangsu Province's Tongshan County all can be as the cultivation base of present method.
4, artificial cultivating method for north Chinese caterpillar fungus with rich strontium according to claim 2 is characterized in that: utilize rich strontium mineral water, underground water to irritate general mulberry, toothed oak trees, raise the silkworm worm with this leaf again, promptly get rich strontium silkworm chrysalis, as the cultivation base.
5, artificial cultivating method for north Chinese caterpillar fungus with rich strontium according to claim 2, it is characterized in that: utilize rich strontium mineral water, underground water to irritate general plant class crop or drunk, with the cultivation base of the animal and plant class material behind the rich strontium as Cordyceps militaris (L.) Link. by the animal class.
6, artificial cultivating method for north Chinese caterpillar fungus with rich strontium according to claim 2 is characterized in that: adopt rich strontium additive, sparge on mulberry, the toothed oak tree and make it rich strontium, utilize rich strontium leaf to raise silkworm larva again.Promptly get rich strontium silkworm chrysalis as the cultivation base.
7, artificial cultivating method for north Chinese caterpillar fungus with rich strontium according to claim 1 and 2 is characterized in that: cultivation based raw material prescription:
(1) choosing rich strontium material is rich strontium rice or other crop rice, rich strontium dried silkworm chrysalis meal etc.
Prescription: rich strontium rice or millet 300-400 gram
Rich strontium dried silkworm chrysalis meal 20-60 gram
Potassium primary phosphate 1-3 gram
Zinc sulfate 0-3 gram
Rich strontium underground water, mineral water 600-700 milliliter
PH value 7-8
(2) utilize the prescription of rich strontium additive:
Rice or millet 300-400 gram
Dried silkworm chrysalis meal 20-60 gram
Water 600-700 milliliter
Potassium primary phosphate 1-3 gram
Zinc sulfate 0-3 gram
Rich strontium additive 1-10 gram
PH value 7-8
Cultivating container adopts resistant to elevated temperatures case, dish, bottle etc., but size variation when making according to the condition of production.
Raw material container inner high voltage sterilization 1-2 hour of packing into connects bacterium, aseptic technique when cooling off back 25 ℃.
Inoculation method: solid spawn utilizes wiping face or suspension system, makes the bacterial classification dispersed, and liquid spawn is adopted spray method, consumption, and 60 centimetres of every length, wide 40 centimetres, high 30 centimetres case are no less than 150 milliliters, the consumption changeable.
After inoculation finishes, indoor temperature control 18-25 ℃, best 20-22 ℃, dark culturing, indoor relative humidity 60-70% saw scattered light after 20 days, and the 40-60 days stromas of gathering add water or nutritive medium in right amount and can receive two to three batches, and biological transformation ratio is more than 100%.
(3) adopt live body silkworm worm to produce rich strontium Cordyceps militaris (L.) Link. method.
Prescription: toothed oak, silkworm worm 50-100 bar
The bright toothed oak of rich strontium, mulberry leaves 500-5000 gram
Cordyceps militaris (L.) Link. bacterium liquid 50-100 milliliter
Silkworm larva is put into case, dish, feed in the time of taking food rich strontium mulberry leaf or toothed oak leaf, 2-3 sprays the Cordyceps militaris (L.) Link. bacterium during age on the silkworm body, 18-23 ℃ of management, relative humidity control 70-80%, make microbiological contamination silkworm body imbed in the soil or the thing of preserving moisture such as wood chip in, can grow stroma, drying or Preservation Treatment in 20-50 days, packing, sealing.
(4) adopt living silkworm chrysalises to produce rich strontium Cordyceps militaris (L.) Link. method.
Prescription: live cocoon chrysalis or mulberry silkworm chrysalis 100-200
Be equal to or greater than the rich strontium spraying fluid of 2PPM 0-100 milliliter
Rich strontium Cordyceps militaris (L.) Link. bacterium liquid 50-200 milliliter
Mulberry silkworm chrysalis or cocoon chrysalis are removed cocoon, and the rich strontium liquid of spray is in pupal cell, after the drying earlier, under sanitary condition, spray into or in pupal cell, inject rich strontium Cordyceps militaris (L.) Link. bacterium liquid with syringe, each pupa injects and sprays into the 0.05-0.5 milliliter, pack in the container after the sterilization, temperature control 15-20 ℃, controlling moisture 70-85%, dark management about 20 days, when pupal cell hardens, be visible scattered light, go out grass, the 30-40 days herbs of can gathering.Dry back Preservation Treatment, packing, sealing is preserved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96100721 CN1156177A (en) | 1996-02-02 | 1996-02-02 | Artificial cultivating method for north Chinese caterpillar fungus with rich strontium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 96100721 CN1156177A (en) | 1996-02-02 | 1996-02-02 | Artificial cultivating method for north Chinese caterpillar fungus with rich strontium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1156177A true CN1156177A (en) | 1997-08-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96100721 Pending CN1156177A (en) | 1996-02-02 | 1996-02-02 | Artificial cultivating method for north Chinese caterpillar fungus with rich strontium |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100389191C (en) * | 2006-06-02 | 2008-05-21 | 广东省微生物研究所 | Highactive wild Chinese caterpillar fungus strain, and its fruiting body and artificial culture method |
| CN104770660A (en) * | 2014-01-13 | 2015-07-15 | 于汇 | Cordyceps sinensis rice with rich cordyceps militaris nutrients and rich medicinal values, and production process thereof |
| CN106957190A (en) * | 2017-04-24 | 2017-07-18 | 广西环江仙草农舍生物科技有限公司 | Cordyceps militaris solid medium |
| CN107155639A (en) * | 2017-07-13 | 2017-09-15 | 山东省蚕业研究所 | A kind of cultural method for the Cordceps militaris for being rich in 1 DNJ |
| CN111972357A (en) * | 2020-08-24 | 2020-11-24 | 普罗欧生物科技(武汉)有限公司 | Method for breeding strontium-rich hermetia illucens larvae |
-
1996
- 1996-02-02 CN CN 96100721 patent/CN1156177A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100389191C (en) * | 2006-06-02 | 2008-05-21 | 广东省微生物研究所 | Highactive wild Chinese caterpillar fungus strain, and its fruiting body and artificial culture method |
| CN104770660A (en) * | 2014-01-13 | 2015-07-15 | 于汇 | Cordyceps sinensis rice with rich cordyceps militaris nutrients and rich medicinal values, and production process thereof |
| CN106957190A (en) * | 2017-04-24 | 2017-07-18 | 广西环江仙草农舍生物科技有限公司 | Cordyceps militaris solid medium |
| CN107155639A (en) * | 2017-07-13 | 2017-09-15 | 山东省蚕业研究所 | A kind of cultural method for the Cordceps militaris for being rich in 1 DNJ |
| CN111972357A (en) * | 2020-08-24 | 2020-11-24 | 普罗欧生物科技(武汉)有限公司 | Method for breeding strontium-rich hermetia illucens larvae |
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