CN1019816B - The artificial culturing method of silkworm chrysalis Cordyceps sinensis - Google Patents
The artificial culturing method of silkworm chrysalis Cordyceps sinensisInfo
- Publication number
- CN1019816B CN1019816B CN87106987A CN87106987A CN1019816B CN 1019816 B CN1019816 B CN 1019816B CN 87106987 A CN87106987 A CN 87106987A CN 87106987 A CN87106987 A CN 87106987A CN 1019816 B CN1019816 B CN 1019816B
- Authority
- CN
- China
- Prior art keywords
- silkworm chrysalis
- link
- cordyceps militaris
- cultivate
- militaris
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Cordyceps militaris (L.) Link. has significant strengthening by means of tonics and anticancer function, be one of famous biological medicine, but resource is extremely scarce.The invention solves Cordyceps militaris (L.) Link. artificial quick cultivation problem, method is that Cordyceps militaris (L.) Link. fungus is inoculated on the substratum, put under certain temperature, wet, the optical condition and cultivate, to add through body surface disinfectant silkworm chrysalis again after a couple of days, maybe this bacterium is made bacterium liquid, inject pupal cell, continue to cultivate then, by the ventilating management of necessity, can obtain artificial silkworm chrysalis Cordyceps sinensis sporophore like a large amount of, effect utmost points consistent after a couple of days with the natural bacteria form, significant to opening up precious medicine source and revitalizing sericulture.
Description
The invention belongs to the artificial culture field of precious biological medicine.
Cordyceps militaris (L.) Link. (cordyceps militaris(L.) Link.) colonizes in pupa, grass (sporophore) complex body that forms behind the insect pupal cells such as lepidopteran for Cordyceps sinensis fungus.Include compositions such as cordycepin (Cordycepin), amino acid, polysaccharide, significant strengthening by means of tonics of tool and anticancer function are one of famous biological medicine.
Because the wild cordyceps militaris resource is extremely scarce, artificial culture shows important working days.The invention solves Cordyceps militaris (L.) Link. fungus is inoculated on tussah, the silkworm pupal cell, and make it to grow consistent with the natural bacteria form.Cordyccps-militaris-(L.)-link. Sporophore problem like the pharmacological action utmost point.Concrete grammar is as follows:
At first be, gather wild cordyceps militaris, separate and purifying bacterial strain (or directly using ready-made bacterial classification) with ordinary method then, it is inoculated on the simple culture media of compositions such as containing protein, carbohydrate and VITAMIN again, put normal temperature, wet, cultivate under the optical condition.Treat that mycelia length arrives to a certain degree, will put into through body surface disinfectant silkworm chrysalis again, continue down to cultivate, can obtain the artificial cordyceps militaris fruiting body after a couple of days in the same terms.
If adopt rice (especially with boiled rice for well) substratum.Interior vitaminize B, amount is approximately about 5 milligrams/1000 grams, puts 20 ℃～25 ℃, sooner or later prolongs illumination (intensity is about 1600Lux) about 4 hours time under the condition, and then culture effect is better.
Be pick up speed and reducing cost, the also available liquid nutrient medium rich medium of agar (as remove) is cultivated this bacterium.Make bacterium liquid (mycelia of soon cultivating 5-7 days is inserted and cultivated in this liquid nutrient medium 5-7 days again), be injected into then through in the body surface sterilization silkworm chrysalis body of (as soaking 3-5 minute) (about 0.1 milliliter /) with neutral spirits.The silkworm chrysalis that will inject bacterium liquid is put into empty bottle at last, adds water about 2 milliliters to keep humidity.And seal with plastics film, under above-mentioned temperature, optical condition, to cultivate, dazzling ventilation on the film that seals when treating that fruit body primordium forms can obtain Cordyccps-militaris-(L.)-link. Sporophore after a couple of days.
Silkwork-Industry Science Inst., Jlin Prov. not collects the fresh wild Cordyceps militaris (L.) Link. in August, 1987 under the mountain forest of soil mountain range, Jiu Tai county, the application organizes separation method has obtained the pure bacterial strain of Cordyceps militaris (L.) Link. (CGMCC No.0172).Utilize this bacterial strain to prepare bacterium liquid.Method is that this bacterium is inoculated on the rich medium, put 20-25 ℃, sooner or later prolong to rise under illumination (intensity is 1600Lux) the 4 hours time condition and cultivated 5 days, be transferred on the liquid rich medium that removes agar, it is 6 millimeters 2 of bacterial classifications that per 30 milliliters of nutrient solutions add diameter, put under the similarity condition and cultivated 7 days, make bacterium liquid, with silkworm chrysalis concentration is 75% carried out body surface in alcohol-pickled 5 minutes and disinfect, again for several times and dry with aseptic water washing, afterwards, inject above-mentioned bacterium liquid with syringe in silkworm chrysalis body segment place.According to every amount of pupal cell size is the 0.05-0.1 milliliter, in the sky Cans of packing into then, adds water 1-2 milliliter, and seals with layer of plastic film, places with above-mentioned temperature, light the same terms to cultivate down.During fruit body primordium to be formed, dazzling ventilation on the film that seals has obtained a large amount of Cordyccps-militaris-(L.)-link. Sporophore after 15 days.
The used cordyceps militaris link bacterial strain of the present invention is preserved in Microbe Inst., Chinese Academy of Sciences common micro-organisms preservation center on June 17th, 1991, and preserving number is CGMCC No.0172.
Claims (4)
1, a kind of artificial culturing method of silkworm chrysalis Cordyceps sinensis, it is characterized in that Cordyceps militaris spawn [Cordyceps militaris (L) Link] (CGMCC No.0172) is inoculated on the solid rich medium of compositions such as containing protein, carbohydrate, vitamins B, put under normal temperature, wet, the optical condition and cultivate, to put into through the silkworm chrysalis that body surface is disinfected after a couple of days, continue to be cultured to the acquisition Cordyccps-militaris-(L.)-link. Sporophore.
2, according to the said method of claim 1, it is characterized in that this bacterial classification inoculation in the liquid rich medium, preparation bacterium liquid, be injected into then in the silkworm chrysalis, in the empty bottle of again it being packed into, add about 2 milliliters in water, seal with plastics film, place then under above-mentioned temperature, the optical condition and cultivate, and dazzling ventilation on the plastics film that seals when growing fruit body primordium, until growing Cordyccps-militaris-(L.)-link. Sporophore.
3, according to the said method of cultivation of claim 1, it is characterized in that said substratum is a rice medium, interior vitaminize B, amount is about about 5 milligrams/1000 grams; Culture temperature is 20 ℃-25 ℃, and the illumination of employing is for sooner or later prolonging light application time about 4 hours (about light intensity 1600Lux).
4,, it is characterized in that said silkworm chrysalis is to carry out the body surface sterilization in about 5 minutes with the neutral spirits immersion according to claim 1 or 2 described methods.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87106987A CN1019816B (en) | 1987-10-13 | 1987-10-13 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN87106987A CN1019816B (en) | 1987-10-13 | 1987-10-13 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1034390A CN1034390A (en) | 1989-08-02 |
| CN1019816B true CN1019816B (en) | 1992-12-30 |
Family
ID=4815925
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN87106987A Expired CN1019816B (en) | 1987-10-13 | 1987-10-13 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1019816B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091149C (en) * | 1993-09-24 | 2002-09-18 | 赵凤英 | Culture method of Cordyceps sinensis and its product |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100392063C (en) * | 2005-11-16 | 2008-06-04 | 广东省微生物研究所 | Pupa Cordyceps sinensis fruiting body using sulphur pupa as host and cultivating process thereof |
| CN100425689C (en) * | 2006-11-06 | 2008-10-15 | 广东省昆虫研究所 | Pupa cordyceps fruiting body cultured using large galleria waxmoth larva and its culturing method |
| CN101210223B (en) * | 2006-12-27 | 2011-07-27 | 近藤幸盛 | Method for producing insect parasitic bacteria fruit body |
| CN101338278B (en) * | 2008-08-07 | 2012-11-21 | 王立臣 | Standard operation method for producing Cordycepsmilitaris of Chinese medicine |
| CN101627701B (en) * | 2009-08-14 | 2010-12-08 | 郑杰辉 | Method for breeding cordyceps militars strain |
| CN102440149B (en) * | 2011-10-10 | 2013-01-23 | 江苏科技大学 | Method for breeding cordyceps militaris by utilizing silkworm larvae |
| CN102907256B (en) * | 2012-10-19 | 2014-03-19 | 湖北省农业科学院农产品加工与核农技术研究所 | Box-type cultivation method of tussah pupa cordyceps militaris |
| CN103704022A (en) * | 2013-12-20 | 2014-04-09 | 凌中鑫 | Method for cultivating artificial cordyceps sinensis with five-instar silkworms as carrier |
| CN103688759A (en) * | 2013-12-20 | 2014-04-02 | 凌中鑫 | Method for culturing artificial cordyceps sinensis by using silkworm pupas as carriers |
| CN103749149A (en) * | 2013-12-20 | 2014-04-30 | 凌中鑫 | Method for cultivating artificial worm grass with mealworm pupa as carrier |
| CN107409746A (en) * | 2017-05-02 | 2017-12-01 | 徐安义 | A kind of breeding method of silkworm chrysalis Cordyceps sinensis |
| CN107058134A (en) * | 2017-05-20 | 2017-08-18 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris strain and application and its nutrient solution used |
| CN107446828A (en) * | 2017-09-19 | 2017-12-08 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris culture medium |
-
1987
- 1987-10-13 CN CN87106987A patent/CN1019816B/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091149C (en) * | 1993-09-24 | 2002-09-18 | 赵凤英 | Culture method of Cordyceps sinensis and its product |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1034390A (en) | 1989-08-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1019816B (en) | The artificial culturing method of silkworm chrysalis Cordyceps sinensis | |
| CN103270887B (en) | Silkworm chrysalis northern Chinese caterpillar Fungus industrial cultivation technique | |
| Gantar et al. | Colonization of wheat (Triticum vulgare L.) by N2‐fixing cyanobacteria: II. An ultrastructural study | |
| CN101045904A (en) | Aweto sporophore culturing process | |
| CN104871830A (en) | Method for planting cordyceps militaris with bean worms as carriers | |
| CN105103948B (en) | A kind of breeding method of silkworm chrysalis Cordyceps sinensis | |
| CN102630534A (en) | Method for controlling pine wood nematode diseases by utilizing body surface bacteria of American pine wood nematodes | |
| CN1372786A (en) | Efficient industrial cultivation technique of gastrodia tuber | |
| KR101239711B1 (en) | Manufacturing process of lentinus edodes culture | |
| CN106954458A (en) | A kind of implantation methods of Hericium erinaceus | |
| CN100469866C (en) | Taishan mountain cordyceps sinensis and artificial cultivation method thereof | |
| CN110140591A (en) | Hickory chick superior strain and its application | |
| Vetayasuporn | Oyster mushroom cultivation on different cellulosic substrates | |
| Gooding Jr et al. | Effect of temperature on growth and survival of Fomes annosus | |
| CN103688760A (en) | Method for culturing artificial cordyceps sinensis by using yellow mealworms as carriers | |
| CN108207484A (en) | A kind of breeding method of silkworm chrysalis cordyceps sinensis | |
| CN113528406B (en) | Brevibacillus brevis ADT and application thereof | |
| CN113040000B (en) | Phellinus igniarius cultivation method capable of achieving fast Phellinus igniarius emergence | |
| CN1637133A (en) | Banana endophyte and its use | |
| CN103749149A (en) | Method for cultivating artificial worm grass with mealworm pupa as carrier | |
| KR980000013A (en) | Culture of Insect Mushroom Species Using Insect | |
| CN207678485U (en) | A kind of two-phase film reactor of bat moth larvae inoculum bacterium source | |
| CN1156177A (en) | Artificial cultivating method for north Chinese caterpillar fungus with rich strontium | |
| Gorelova et al. | Heteromorphism of the cyanobacterium Nostoc sp., a microsymbiont of the Blasia pusilla moss | |
| CN1092105A (en) | The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C13 | Decision | ||
| GR02 | Examined patent application | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |