CN107446828A - A kind of Cordyceps militaris culture medium - Google Patents
A kind of Cordyceps militaris culture medium Download PDFInfo
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- CN107446828A CN107446828A CN201710844822.1A CN201710844822A CN107446828A CN 107446828 A CN107446828 A CN 107446828A CN 201710844822 A CN201710844822 A CN 201710844822A CN 107446828 A CN107446828 A CN 107446828A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The present invention discloses a kind of Cordyceps militaris culture medium, by 0.5 2.5 parts of sucrose, 5.5 15.5 parts of corn flour, 0.5 3 parts of whole milk powder, 3.5 6 parts of wheat bran, 2.5 6 parts of peanut powder, 0.5 2 parts of potassium dihydrogen phosphate, 35 parts of peptone, biphosphate receive 4.5 8.5 parts, 2.5 4 parts of egg white peptide, 0.5 2 parts of brewer's wort, 10 15 parts of water forms, a kind of Cordyceps militaris culture medium of the present invention, survival rate is more preferable, cordycepin content is higher, nutrition price is good, and the output value is high.
Description
Technical field
The present invention relates to cordyceps sinensis to cultivate field, more particularly to a kind of Cordyceps militaris culture medium.
Background technology
Cordyceps militaris physiology is unique, and ecology is complicated, and nature is that the pupal cell of lepidopterous insects infects the worm formed after Cordyceps Militaris
Complex is formed with fungi two parts, is mainly grown in pin, broad-leaf forest or mixed forest surface soil layer.Larva lives in seclusion soil in the winter time
In, mushroom parasitism wherein, draws nutrition, and larva body is interior dead full of mycelia.To summer, children is born above larva corpse
Seedling, likeness in form grass, gathers and obtains before and after the Summer Solstice.Cordyceps militaris NATURAL DISTRIBUTION resource quantity is seldom, expensive.Using Traditional Man
Cultivation, is Cordyceps Militaris to be injected into silkworm chrysalis living, simulating natural condition obtains Cordyceps militaris.But cultivate in this way,
Injection technique is difficult to control, and easily pollution, yields poorly, general peasant household is difficult to operate.In recent years, also there are many ginseng more solito medicines
With the production method of fungi, it is unreasonable culture medium nutrition-allocated proportion occur, a variety of phenomenons such as spawn degeneration, seriously polluted, causes to produce
Amount declines, benefit is relatively low.
The content of the invention
The present invention solves the technical problem of providing a kind of Cordyceps militaris culture medium, cordyceps sinensis survival rate 98% with
On, cordycepin content is higher, and nutrition price is good, and the output value is high.
In order to solve the above technical problems, one aspect of the present invention is：
A kind of Cordyceps militaris culture medium, by sucrose, corn flour, whole milk powder, wheat bran, peanut powder, potassium dihydrogen phosphate, albumen
Peptone, biphosphate are received, egg white peptide, brewer's wort, water composition, and each component is by weight：Sucrose 0.5-2.5 parts, corn flour 5.5-
15.5 parts, whole milk powder 0.5-3 parts, wheat bran 3.5-6 parts, peanut powder 2.5-6 parts, potassium dihydrogen phosphate 0.5-2 parts, peptone 3-5
Part, biphosphate receive 4.5-8.5 parts, egg white peptide 2.5-4 parts, brewer's wort 0.5-2 parts, water 10-15 parts.
In a preferred embodiment of the present invention, the water is the water of pH value 6.5 ~ 7.
In a preferred embodiment of the present invention, the compound of zinc, magnesium, calcium is mixed with the water.
In a preferred embodiment of the present invention, each component is by weight in the Cordyceps militaris culture medium：Sucrose 0.5
Part, 5.5 parts of corn flour, 0.5 part of whole milk powder, 3.5 parts of wheat bran, 2.5 parts of peanut powder, 0.5 part of potassium dihydrogen phosphate, 3 parts of peptone,
Biphosphate receive 4.5 parts, 2.5 parts of egg white peptide, 0.5 part of brewer's wort, 10 parts of water.
In a preferred embodiment of the present invention, each component is by weight in the Cordyceps militaris culture medium：Sucrose 1.5
Part, 8 parts of corn flour, 2 parts of whole milk powder, 4 parts of wheat bran, 5 parts of peanut powder, 1 part of potassium dihydrogen phosphate, 4 parts of peptone, biphosphate are received
6 parts, 3 parts of egg white peptide, 1.5 parts of brewer's wort, 12 parts of water.
In a preferred embodiment of the present invention, each component is by weight in the Cordyceps militaris culture medium：Sucrose 2.5
Part, 15.5 parts of corn flour, 3 parts of whole milk powder, 6 parts of wheat bran, 6 parts of peanut powder, 2 parts of potassium dihydrogen phosphate, 5 parts of peptone, di(2-ethylhexyl)phosphate
Hydrogen receive 8.5 parts, 4 parts of egg white peptide, 2 parts of brewer's wort, 15 parts of water.
The beneficial effects of the invention are as follows：A kind of Cordyceps militaris culture medium of the present invention, cordyceps sinensis survival rate more than 98%, worm
Careless cellulose content is higher, and nutrition price is good, and the output value is high.
Embodiment
The technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described implementation
Example is only the part of the embodiment of the present invention, rather than whole embodiments.It is common based on the embodiment in the present invention, this area
All other embodiment that technical staff is obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.
The embodiment of the present invention includes：
A kind of Cordyceps militaris culture medium, by sucrose, corn flour, whole milk powder, wheat bran, peanut powder, potassium dihydrogen phosphate, albumen
Peptone, biphosphate are received, egg white peptide, brewer's wort, water composition, and each component is by weight：Sucrose 0.5-2.5 parts, corn flour 5.5-
15.5 parts, whole milk powder 0.5-3 parts, wheat bran 3.5-6 parts, peanut powder 2.5-6 parts, potassium dihydrogen phosphate 0.5-2 parts, peptone 3-5
Part, biphosphate receive 4.5-8.5 parts, egg white peptide 2.5-4 parts, brewer's wort 0.5-2 parts, water 10-15 parts.
In above-mentioned, the water is the water of pH value 6.5 ~ 7.
In above-mentioned, the compound of zinc, magnesium, calcium is mixed with the water.
Further, each component is by weight in the Cordyceps militaris culture medium：0.5 part of sucrose, 5.5 parts of corn flour,
0.5 part of whole milk powder, 3.5 parts of wheat bran, 2.5 parts of peanut powder, 0.5 part of potassium dihydrogen phosphate, 3 parts of peptone, biphosphate receive 4.5
Part, 2.5 parts of egg white peptide, 0.5 part of brewer's wort, 10 parts of water.
Further, each component is by weight in the Cordyceps militaris culture medium：It is 1.5 parts of sucrose, 8 parts of corn flour, complete
2 parts of fat milk powder, 4 parts of wheat bran, 5 parts of peanut powder, 1 part of potassium dihydrogen phosphate, 4 parts of peptone, biphosphate receive 6 parts, 3 parts of egg white peptide,
1.5 parts of brewer's wort, 12 parts of water.
Further, each component is by weight in the Cordyceps militaris culture medium：2.5 parts of sucrose, 15.5 parts of corn flour,
3 parts of whole milk powder, 6 parts of wheat bran, 6 parts of peanut powder, 2 parts of potassium dihydrogen phosphate, 5 parts of peptone, biphosphate receive 8.5 parts, egg white peptide 4
Part, 2 parts of brewer's wort, 15 parts of water.
The beneficial effect of Cordyceps militaris culture medium of the present invention is：A kind of Cordyceps militaris culture medium of the present invention, cordyceps sinensis into
Motility rate is up to more than 98%, and cordycepin content is higher, and nutrition price is good, and the output value is high.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalent structure or equivalent flow conversion that bright description is made, or directly or indirectly it is used in other related technology necks
Domain, it is included within the scope of the present invention.
Claims (6)
1. a kind of Cordyceps militaris culture medium, it is characterised in that by sucrose, corn flour, whole milk powder, wheat bran, peanut powder, phosphorus
Acid dihydride potassium, peptone, biphosphate are received, egg white peptide, brewer's wort, water composition, and each component is by weight：Sucrose 0.5-2.5 parts,
Corn flour 5.5-15.5 parts, whole milk powder 0.5-3 parts, wheat bran 3.5-6 parts, peanut powder 2.5-6 parts, potassium dihydrogen phosphate 0.5-2 parts,
Peptone 3-5 parts, biphosphate receive 4.5-8.5 parts, egg white peptide 2.5-4 parts, brewer's wort 0.5-2 parts, water 10-15 parts.
2. Cordyceps militaris culture medium according to claim 1, it is characterised in that the water is the water of pH value 6.5 ~ 7.
3. Cordyceps militaris culture medium according to claim 2, it is characterised in that zinc, magnesium, calcium are mixed with the water
Compound.
4. Cordyceps militaris culture medium according to claim 1, it is characterised in that in the Cordyceps militaris culture medium
Each component is by weight：0.5 part of sucrose, 5.5 parts of corn flour, 0.5 part of whole milk powder, 3.5 parts of wheat bran, 2.5 parts of peanut powder, phosphoric acid
0.5 part of potassium dihydrogen, 3 parts of peptone, biphosphate receive 4.5 parts, 2.5 parts of egg white peptide, 0.5 part of brewer's wort, 10 parts of water.
5. Cordyceps militaris culture medium according to claim 1, it is characterised in that in the Cordyceps militaris culture medium
Each component is by weight：1.5 parts of sucrose, 8 parts of corn flour, 2 parts of whole milk powder, 4 parts of wheat bran, 5 parts of peanut powder, potassium dihydrogen phosphate 1
Part, 4 parts of peptone, biphosphate receive 6 parts, 3 parts of egg white peptide, 1.5 parts of brewer's wort, 12 parts of water.
6. Cordyceps militaris culture medium according to claim 1, it is characterised in that in the Cordyceps militaris culture medium
Each component is by weight：2.5 parts of sucrose, 15.5 parts of corn flour, 3 parts of whole milk powder, 6 parts of wheat bran, 6 parts of peanut powder, biphosphate
2 parts of potassium, 5 parts of peptone, biphosphate receive 8.5 parts, 4 parts of egg white peptide, 2 parts of brewer's wort, 15 parts of water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201710844822.1A CN107446828A (en) | 2017-09-19 | 2017-09-19 | A kind of Cordyceps militaris culture medium |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710844822.1A CN107446828A (en) | 2017-09-19 | 2017-09-19 | A kind of Cordyceps militaris culture medium |
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| CN107446828A true CN107446828A (en) | 2017-12-08 |
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| CN201710844822.1A Pending CN107446828A (en) | 2017-09-19 | 2017-09-19 | A kind of Cordyceps militaris culture medium |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112154861A (en) * | 2020-10-16 | 2021-01-01 | 杭州科迪赛生物科技有限公司 | Cordyceps militaris culture medium and preparation method and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034390A (en) * | 1987-10-13 | 1989-08-02 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1092105A (en) * | 1994-02-26 | 1994-09-14 | 苏州蚕桑专科学校 | The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. |
| CN1095104A (en) * | 1993-05-13 | 1994-11-16 | 刘文霞 | Cordyccps-militaris-(L.)-link. Sporophore and mycelium batch production production technology |
| CN1167825A (en) * | 1996-05-27 | 1997-12-17 | 日原町 | Method for artificial culture of cordyceps, e.g. Cordyceps mititaris |
| CN104928190A (en) * | 2015-06-11 | 2015-09-23 | 天津耀宇生物技术有限公司 | Cordyceps militaris fluid medium and preparation method thereof |
-
2017
- 2017-09-19 CN CN201710844822.1A patent/CN107446828A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034390A (en) * | 1987-10-13 | 1989-08-02 | 吉林省蚕业科学研究所 | The artificial culturing method of silkworm chrysalis Cordyceps sinensis |
| CN1095104A (en) * | 1993-05-13 | 1994-11-16 | 刘文霞 | Cordyccps-militaris-(L.)-link. Sporophore and mycelium batch production production technology |
| CN1092105A (en) * | 1994-02-26 | 1994-09-14 | 苏州蚕桑专科学校 | The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. |
| CN1167825A (en) * | 1996-05-27 | 1997-12-17 | 日原町 | Method for artificial culture of cordyceps, e.g. Cordyceps mititaris |
| CN104928190A (en) * | 2015-06-11 | 2015-09-23 | 天津耀宇生物技术有限公司 | Cordyceps militaris fluid medium and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112154861A (en) * | 2020-10-16 | 2021-01-01 | 杭州科迪赛生物科技有限公司 | Cordyceps militaris culture medium and preparation method and application thereof |
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