CN1092105A - The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. - Google Patents
The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. Download PDFInfo
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- CN1092105A CN1092105A CN 94112056 CN94112056A CN1092105A CN 1092105 A CN1092105 A CN 1092105A CN 94112056 CN94112056 CN 94112056 CN 94112056 A CN94112056 A CN 94112056A CN 1092105 A CN1092105 A CN 1092105A
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Abstract
The present invention relates to artificial cultivating method of pupa worm grass.It is characterized in that the conidium of Cordyceps militaris (L.) Link. fungus is made the spore suspension liquid, dip in insect needle or analogue and to get bacterium liquid, thrust silkworm chrysalis, treat vegetative hyphae fully growth in vivo, after silkworm chrysalis is stiff, cover one deck thereon or stiff pupa is inserted on the porose material of easily preserving moisture with porous materials, under suitable temperature, humidity, light, cultivate and to obtain a large amount of Cordyceps militaris (L.) Link..The Cordyceps militaris (L.) Link. that the method for using is turned out, the fermented product of its pharmaceutical component and wild cordyceps, Cordyceps fungus do not have essence matter difference.
Description
The present invention relates to the artificial culturing method of a kind of microorganism, particularly Cordyceps militaris (L.) Link. (Cordyceps militaris(L) Link) the high-yield quick artificial culturing method.
Cordyceps sinensis (Cordyceps sinensis Sace) is a kind of of Cordyceps sinensis fungus, originate in China Sichuan, Tibet, Qinghai, Yunnan etc. more than the provinces and regions height above sea level 3000 granular snow lines, because of the restriction and the over-drastic of ecotope are excavated, resource is day by day exhausted, far can not satisfy the demand, therefore domestic and international many scholars all attempt by being seeded in artificially cultivating cordyceps on the bat larva, but difficulty is very big.In recent years, many scholars just are being devoted to the industrial fermentation of Cordyceps fungus and are seeking the substitute of Cordyceps sinensis in other Chinese caterpillar funguses.
Cordyceps militaris (L.) Link. has another name called Cordyceps militaris (L.) Link. or Cordyceps militaris (L.) Link., is the type species of Cordyceps.The same Cordyceps sinensis of drug effect, but wild cordyceps militaris quantity is few, therefore cultivate the Cordyceps militaris (L.) Link. with natural form very big potential market is arranged: the CN1034390A patent application discloses a kind of artificial culturing method of silkworm chrysalis Cordyceps sinensis, it is to grow stroma with Cordyceps militaris (L.) Link. fungus on solid medium, and then be the host with the tussah, the silkworm chrysalis input length that the body surface detoxicating is crossed in employing has on the substratum of Cordyceps militaris (L.) Link. fungus or uses to pupal cell and inject this dual mode of liquid culture bacterium liquid, make pupal cell infect Cordyceps militaris (L.) Link. fungus, metainfective pupal cell is put in the empty bottle and is cultivated, and obtains the Cordyceps militaris (L.) Link. with natural form.But this cultural method has obvious weak point: (1) pupal cell table sterilization method complexity; (2) will have on the substratum of Cordyceps militaris (L.) Link. fungus through the silkworm chrysalis input length that body surface is disinfected, need a large amount of substratum, and easily cause living contaminants; (3) inject liquid culture bacterium liquid to pupal cell, troublesome poeration and the sepsis pupa easily takes place; (4) pupal cell behind the infection Cordyceps militaris (L.) Link. fungus is put in the empty bottle and is cultivated, and the stroma of turning out is lacked and easily come off.
The present invention is intended to seek a kind of Cordyceps militaris (L.) Link. high-yield quick artificial culturing method that overcomes above method shortcoming.
Technical scheme of the present invention is: the Cordyceps militaris (L.) Link. fungus after will screening is inoculated in and contains on the proteinic substratum, under indoor suitable temperature and available light, is cultured to and forms a large amount of conidiums, and the picking conidium is made the spore suspension liquid with sterilized water; This bacterium liquid is inoculated in the host, and protection is to stiff; With stiff pupa, under simulation Cordyceps militaris (L.) Link. natural ecological condition, be cultured to the stroma maturation.
With the artificial Cordyceps militaris (L.) Link. of cultivating of the present invention, from being inoculated into stroma ripe about 35～45 days, infection success ratio, the stroma rate of growing of Cordyceps militaris (L.) Link. fungus reach more than 95%, and every square metre cultivated area can be received dried Cordyceps militaris (L.) Link. 250～300 grams, the same wild Chinese caterpillar fungus of its outward appearance of the stroma that grows from silkworm chrysalis, stroma is orange, sturdy, long 3～8 centimetres, thick 0.2～0.8 centimetre, idol has bifurcated, each pupal cell 1～3 root seat, stroma can reach 1: 1.5 with pupal cell dry weight ratio～and 2.3.
Table 1 is that the silkworm chrysalis Cordyceps sinensis and the Cordyceps sinensis effective component content that adopt this law to cultivate compare
Below in conjunction with embodiment the present invention is further described.
Concrete operation method of the present invention is as follows: the screening of (1) bacterial strain: Cordyceps militaris (L.) Link. repeatedly filters out the silkworm chrysalis virulence after the rejuvenation strong through the pupal cell of living, and easily forms the bacterial strain of stroma.(2) the spore suspension liquid is made: Cordyceps militaris (L.) Link. fungus is containing on the proteinic substratum, is cultured under 22 ℃ and indoor natural light line and forms a large amount of conidiums, and the picking conidium is made the spore suspension liquid with sterilized water.(3) host prepares: silkworm or the Semen Ricini silkworm back sootiness detoxicating of being placed on small straw bundles to spin cocoons, cut open cocoon after the week and get pupa, reject disease pupa and bad pupa, select the strong pupa of suitable developmental stage to do the host, or directly get before putting on an arrow five the later stage in age larva do the host.(4) infection of Cordyceps militaris (L.) Link. fungus: dip in the pointed part of insect needle or analogue and to get the spore suspension liquid of making as (2) method, thorn people pupal cell, every thorn one pin.(5) protection of infection back pupal cell: metainfective pupal cell is divided in silkworm plaque or similar frame, and thermophilic protects pupal cell stiff, rejects the sepsis pupa.(6) cultivation of stroma: under the natural ecological environment of simulation Cordyceps militaris (L.) Link., cultivate stiff pupa to the stroma maturation, its method is to adopt porous materials (as cinder, broken sponge etc.) to cover one deck on stiff pupal cell, more than 90%, indoor natural light lines spare is cultivated down in thermophilic and relative temperature.(7) oriented growth of stroma: in order to overcome the many and thin shortcoming of stroma that pupal cell grows, make stroma long and abundant, can adopt the post-directed training of stroma, its method is: with stiff pupa head up, insert porose (also can manually punch) easily on material of insulation (as beehive coal ball or sponge of having openning hole in advance), in above-mentioned humiture, cultivate under the light condition.(8) collection of Cordyceps militaris (L.) Link. is preserved: after the Cordyceps militaris (L.) Link. maturation, dig out cleaning, with edible wine body surface sterilization, arrangement stroma, lyophilize or 60 ℃ are sealed in shady and cool dry place after the oven dry down and preserve.
The silkworm chrysalis Cordyceps sinensis that this law is cultivated shows through system's thin-layer chromatography: contained fat-soluble composition, sterol, N.F,USP MANNITOL, purine, pyrimidine, alkaloid, the quantity of total free aminoacids, size, the Rf value of silkworm chrysalis Cordyceps sinensis is corresponding with Cordyceps sinensis.Quantitative analysis results is as shown in table 1, and silkworm chrysalis Cordyceps sinensis and Cordyceps sinensis is no essence matter difference on Chemical Composition, but the content of active ingredient cordycepin, VITAMIN B4, silkworm chrysalis Cordyceps sinensis is 3 times of Cordyceps sinensis.With the content in the Cordyceps fungus fermented product of relevant bibliographical information relatively, silkworm chrysalis Cordyceps sinensis still has obvious superiority.Acute toxicity test shows: silkworm chrysalis Cordyceps sinensis is pressed toxicity category " nontoxic substantially " owned by France level.Salmonella reversion test shows: the silkworm chrysalis worm that this law is cultivated does not see and presents the mutagenesis positive effect.Mouse sperm distortion test shows that the silkworm chrysalis Cordyceps sinensis that this law is cultivated does not have teratogenesis to mouse sperm.Mouse marrow cell micro nuclear test shows that the silkworm chrysalis Cordyceps sinensis that this law is cultivated does not have influence to mouse bone marrow cells micronucleus occurrence rate.
Claims (3)
1, a kind of Cordyceps militaris (L.) Link. high-yield quick artificial culturing method; it is characterized in that: (Cordyceps militaris (L) Link) is inoculated on the substratum with Cordyceps militaris (L.) Link. fungus; under indoor thermophilic and available light; be cultured to and form a large amount of conidiums; the picking conidium is made the spore suspension liquid with sterilized water; this bacterium liquid is inoculated in the host, and protection is to stiff, and stiff pupa is cultured to Cordyceps militaris (L.) Link. stroma maturation under simulation Cordyceps militaris (L.) Link. natural ecological condition.
2, according to the described method of cultivation of claim 1, it is characterized in that: described simulation Cordyceps militaris (L.) Link. natural ecological condition is meant with porous material and covers one deck infecting on the stiff pupa of Cordyceps militaris (L.) Link. fungus, or stiff pupa inserted the material of porose easy insulation, thermophilic and the relative temperature indoor natural light lines spare more than 90%.
3, according to claim 1 or 2 described method of cultivation, it is characterized in that with bacterium liquid be inoculated in the host be with insect needle or its analogue libation at an ancient wedding ceremony get bacterium liquid thrust silkworm chrysalis or five the later stage in age larva.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94112056 CN1092105A (en) | 1994-02-26 | 1994-02-26 | The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94112056 CN1092105A (en) | 1994-02-26 | 1994-02-26 | The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. |
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| Publication Number | Publication Date |
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| CN1092105A true CN1092105A (en) | 1994-09-14 |
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| CN 94112056 Pending CN1092105A (en) | 1994-02-26 | 1994-02-26 | The high-yield quick artificial culturing method of Cordyceps militaris (L.) Link. |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000201659A (en) * | 1999-01-14 | 2000-07-25 | Nichiharachiyou | Tochukaso tea |
| CN1117853C (en) * | 1996-12-05 | 2003-08-13 | 大韩民国农村振兴厅 | Cultivation of paecilomyces species, using silkworms |
| CN100469866C (en) * | 2005-04-30 | 2009-03-18 | 泰安市农业科学研究院 | Mountain Tai aweto and artificial culture method thereof |
| CN102498947A (en) * | 2011-11-07 | 2012-06-20 | 许继宏 | Method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae |
| CN102942422A (en) * | 2012-12-13 | 2013-02-27 | 重庆慈恩生物科技有限公司 | Culture medium for liquid state cultivation of cordyceps militaris sporocarp and culturing method thereof |
| CN105359825A (en) * | 2013-05-15 | 2016-03-02 | 刘东波 | Silkworm insect grass cultivating method of infecting bombyxmori larva by cordyceps fungus strain |
| CN107446828A (en) * | 2017-09-19 | 2017-12-08 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris culture medium |
-
1994
- 1994-02-26 CN CN 94112056 patent/CN1092105A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1117853C (en) * | 1996-12-05 | 2003-08-13 | 大韩民国农村振兴厅 | Cultivation of paecilomyces species, using silkworms |
| JP2000201659A (en) * | 1999-01-14 | 2000-07-25 | Nichiharachiyou | Tochukaso tea |
| CN100469866C (en) * | 2005-04-30 | 2009-03-18 | 泰安市农业科学研究院 | Mountain Tai aweto and artificial culture method thereof |
| CN102498947A (en) * | 2011-11-07 | 2012-06-20 | 许继宏 | Method for culturing cordyceps sinensis by performing endogenous pressurization and artificial infection of hirsutella sinensis on hepialus larvae |
| CN102942422A (en) * | 2012-12-13 | 2013-02-27 | 重庆慈恩生物科技有限公司 | Culture medium for liquid state cultivation of cordyceps militaris sporocarp and culturing method thereof |
| CN105359825A (en) * | 2013-05-15 | 2016-03-02 | 刘东波 | Silkworm insect grass cultivating method of infecting bombyxmori larva by cordyceps fungus strain |
| CN107446828A (en) * | 2017-09-19 | 2017-12-08 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris culture medium |
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