CN1152132C - Method for artificially cultivating cordyceps - Google Patents
Method for artificially cultivating cordyceps Download PDFInfo
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- CN1152132C CN1152132C CNB001084860A CN00108486A CN1152132C CN 1152132 C CN1152132 C CN 1152132C CN B001084860 A CNB001084860 A CN B001084860A CN 00108486 A CN00108486 A CN 00108486A CN 1152132 C CN1152132 C CN 1152132C
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Abstract
The present invention relates to a method for artificially culturing Chinese caterpillar fungus. First, strains are inoculated in a culture medium prepared from rice, corn, bran coats, silkworm kidney powder and nutrient fluid prepared from water, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, triamine citrate and vitamin B1; then the strains are cultured for 20 to 25 days at the temperature of 12 DEG C to 17 DEG C under the environment of protection from light; after that the strains are cultured for 10 day for changing the color under the 200 lux of illumination, and the growth of fruiting bodies is stimulated under the temperature difference of 10 DEG C between the day and the night, and then the fruiting bodies are picked and collected. Through detection, no obvious differences exist between the chemical components and the pharmacology and pharmacological effect of artificial Chinese caterpillar fungus and that of natural Chinese caterpillar fungus; artificial Chinese caterpillar fungus has higher yield, and can greatly satisfy the people's requirements for Chinese caterpillar fungus, which grow increasingly.
Description
The invention belongs to the fungi artificial breeding technique, relate to a kind of method of artificially cultivating cordyceps.
Cordyceps sinensis is a kind of extremely precious medicine-food two-purpose fungi, is categorized as Ascomycetes, Hypocreales, Clavicipitaceae, grass-and-insect painting genus on mycology.Wild cordyceps all colonizes in the lepidopterous insects body, and growing environment is had relatively high expectations, so its output is subjected to very big restriction.Along with the development of society and the raising of people's living standard, particularly to the further excavation of Cordyceps sinensis medicine effect, its demand is increasing, only depends on the collection wild cordyceps far can not satisfy needs of society.At present, the report of artificial culture only is under the test tube condition of laboratory seldom, or adopts fermentation process to produce the method for Cordyceps mycelium.As CN1197843A disclosed " fermentation method for producing of Chinese Cordyceps sinensis fungi ", adopt liquid nutrient medium to carry out fermentative production exactly, its product does not have sporophore.
The objective of the invention is method, cultivate Cordyceps sporophore,, satisfy people's needs to improve output with its growth conditions of manual simulation.
The culture medium raw material that the present invention adopts is rice, corn, cavings, dried silkworm chrysalis meal and by water, glucose, peptone, potassium primary phosphate, sal epsom, Triammonium citrate, vitamins B
1Formulated nutritive medium.The prescription of nutritive medium is: add glucose 10 grams, peptone 5 grams, potassium primary phosphate 2 grams, sal epsom 1 gram, Triammonium citrate 1 gram, vitamins B in per 1000 ml waters
110 milligrams.Culture medium raw material composition weight proportioning is: rice 20-25%, corn 10-15%, cavings 4-8%, dried silkworm chrysalis meal 1-2%, nutritive medium 50-55%.
Method of cultivation of the present invention (step):
1, the making of substratum.In container, add rice, corn, cavings, dried silkworm chrysalis meal and nutritive medium according to the above ratio, container closure, standby with ordinary method sterilization postcooling.
2, inoculation.Under no bacterium condition, operate, Cordyceps fungus is inserted in the substratum.
3, bacteria.Place 12-17 degree centigrade lucifuge environment to cultivate 20-25 days down in the container that inserts bacterium, cover with substratum more than 2/3 until white hypha.
4, annesl.Continuing cultivation about 10 days under the illumination condition more than 200 luxs, mycelia transfers yellow to by white.
5, stimulate the generation of fruit body primordium.Stimulated 10 days under the condition of day and night temperature more than 10 degrees centigrade behind the annesl, media surface produces a large amount of fruit body primordiums.Day and night temperature scope: 5-25 degree centigrade, in the sealing part perforation, make the culture space proper ventilation, simultaneously to supply with competent oxygen.
6, sporophore growth.Under 18-25 degree centigrade, the condition of relative humidity 85-90%, the stable cultivation 20-25 days, sporophore covers with container, and length reaches 6-7cm.
7, pluck receipts.Open and seal, sporophore is shoveled down gently In Shade airing or oven dry from root with the little spades of being with elbow.
Remarkable advantage of the present invention and positively effect:
1, the Cordyceps sinensis of artificial culture is through the check of Chinese green food association, and is basic identical with the chemical ingredients of natural cordyceps; The amino acid kind is identical, and its content major part exceeds one times than natural cordyceps sinensis.Concrete composition sees attached list 1.
Inorganic elements has 15 kinds of elements identical with natural cordyceps sinensis in 26 kinds of inorganic elementss measuring, and content is very little; The content of all the other 11 kinds of elements is higher, and concrete content is listed subordinate list 2 in.
Wherein Ca, Al, Si, Zn, Cr are a little more than natural cordyceps, and Ti, Cu are a little less than natural cordyceps, and Fe is than the low 270ppm of natural worm summer in winter worm, and Mn hangs down 45ppm, and heavy metal content is no more than 15ppm.
Nucleosides nucleoside compound content artificial and natural cordyceps is more approaching, but artificial aweto contains adenosine more.Concrete content is listed subordinate list 3 in.
The ergosterol content of ergosterol and D-N.F,USP MANNITOL submitted sample exceeds one times than natural cs, is respectively 0.15% and 0.069%; The D-mannitol content is then low by 30% than natural cs, is respectively 5.5% and 8.1%.
VITAMIN riboflavin content is basic identical, is 1.25mg/100g; VitB1 content is more much higher than natural cs, is respectively 0.34mg/100g and 0.018mg/100g; β-carotenoids content is 0.76mg/100g, and does not detect in the natural cs; Vitamin C content is significantly less than natural cs, is respectively 4mg/100g and 23.1mg/100g.
2, the pharmacological function no significant difference of the Cordyceps sinensis of artificial culture and natural cordyceps.
Select for use U.S. small white mouse to carry out animal experiment, be divided into three groups: I blank, II artificial aweto, III natural cs.Fed respectively continuously 45 days with doses, to its 12 physical signs tests, the result shows that II, III group obviously is better than the I group, no significant difference between II, the III group.Prove that artificial Cordyceps sinensis is the same substantially with natural cordyceps on pharmacological effect, belong to the medicine-food two-purpose high tonic.
3, the method for artificially cultivating cordyceps has higher output, adopts indoor stereo to cultivate, and unit square rice can be adopted 150-200g weekly, has favorable economic benefit.
4, the present invention can greatly satisfy the demand to Cordyceps sinensis that people increase day by day, and protection wild cordyceps resource has bigger social benefit.
Embodiment 1
Substratum is: rice 20%, corn 10%, cavings 4%, dried silkworm chrysalis meal 1%, nutritive medium 50%.Wherein the prescription of nutritive medium is: add glucose 10 grams, peptone 5 grams, potassium primary phosphate 2 grams, sal epsom 1 gram, triammonium citrate 1 gram, vitamins B in 1000 ml waters
110 milligrams.Incubation step: 1, above-mentioned substratum is packed in the container, seal, place 0.1Mpa, 120 degrees centigrade 1.5 hours postcooling of sterilization are standby down with polypropylene plastic film; 2, Cordyceps fungus is inserted in the substratum and (under aseptic condition, operate); 3, the container that will insert bacterial classification places 12 degrees centigrade lucifuge environment to cultivate 20 days down, the growth mycelia; 4, under the illumination condition more than 200 luxs, cultivate 10 days annesls; 5, the mycelium behind the annesl was stimulated 10 days under the condition at (5 degrees centigrade of daytimes, 15 degrees centigrade of nights) more than 10 degrees centigrade at day and night temperature, and with toothpick in sealing part perforation ventilation, produce a large amount of fruit body primordiums; 6, under 18 degrees centigrade, 85% relative humidity good ventilation condition, the stable cultivation 20 days, sporophore covers with container (it is long to reach 6-7cm); 7, open and seal, pluck the receipts sporophore.
Embodiment 2
Substratum is: rice 25%, corn 15%, cavings 8%, dried silkworm chrysalis meal 2%, nutritive medium 55%.Wherein the prescription of nutritive medium is with example 1.Incubation step: 1, preparation substratum (with example 1), 2, inoculation (with example 1), 3,17 degrees centigrade of bacteria envrionment temperatures, lucifuge was cultivated 25 days, and 4, annesl (with example 1), 5, stimulate fruit body primordium to produce.Day and night temperature 10 degrees centigrade (15 degrees centigrade of daytimes, 25 degrees centigrade of nights) stimulated 10 days, and all the other, were cultivated 25 days under relative humidity 90% ventilation condition 25 degrees centigrade of temperature with example 1,6, sporophore growth, and 7, pluck the receipts sporophore.
Subordinate list 1: artificial aweto and natural cs aminoacid ingredient are relatively
Amino acid kind natural cs (%) artificial aweto (%)
Asparatate 1.646 3.449
Threonine 0.911 1.715
Serine 1.080 1.424
Glutamic acid 2.360 4.172
Glycine 0.954 10886
Alanine 1.140 2.698
Cystine 0.381 0.333
Valine 0.719 1.634
Methionine 0.285 0.543
Isoleucine 2.280 5.529
Leucine 0.937 2.821
Tyrosine 0.853 0.384
Phenylalanine 0.781 1.008
Lysine 0.913 2.114
Ammonia 0.339 0.527
Histidine 0.791 0.680
Arginine 1.426 0.479
Proline 1.029 1.465
Subordinate list 2: artificial aweto and natural cs inorganic ion content are relatively
Element term natural cs (%) artificial aweto (%)
P 0.92 0.093
Mg 0.068 0.057
Fe 0.022 0.006
Ca 0.013 0.013
Al 0.0006 0.0051
Si 0.0034 0.0054
Zn 0.0031 0.0041
Mn 0.0014 0.0012
Cu 0.0005 0.0004
Ti 0.0002 does not detect
Cr 0.0001 0.0002
Subordinate list 3: artificial aweto and natural cs glycoside content is (mg/g/% of unit) relatively
Uridylic uridine VITAMIN B4 adenosine Desoxyadenosine
Natural cs 0.243/33.2 0.308/42.1 0.162/22.1--0.0183/2.5
Artificial aweto 0.215/34.7 0.275/44.4 0.105/17.0 0.0116/1.9 0.0121/2.0
Claims (3)
1, a kind of method of artificially cultivating cordyceps is characterized in that: at first the bacterial classification access is reached by water, glucose, peptone, potassium primary phosphate, sal epsom, triammonium citrate, vitamins B with rice, corn, cavings, dried silkworm chrysalis meal
1In the substratum of the nutritive medium that is made into, the container that will insert Cordyceps fungus then places under 12-17 degree centigrade the lucifuge environment, cultivates 20-25 days, bears white hypha, under illumination condition, continue again to cultivate 10 days, mycelium transfers yellow to by white, stimulates 10 days under the condition of 10 degrees centigrade of day and night temperatures behind the annesl, produces fruit body primordium, at temperature 18-25 degree centigrade, under the condition of relative humidity 85-90%, the stable cultivation 20-25 days, receipts are grown up, plucked to sporophore; The composition weight proportioning of substratum is: rice 20-25%, corn 10-15%, cavings 4-8%, dried silkworm chrysalis meal 1-2%, nutritive medium 50-55%, wherein the prescription of nutritive medium is: add glucose 10 grams, peptone 5 grams, potassium primary phosphate 2 grams, sal epsom 1 gram, triammonium citrate 1 gram, vitamins B in per 1000 ml waters
110 milligrams.
2, according to the method for the described artificially cultivating cordyceps of claim 1, it is characterized in that: described illumination condition is greater than 200 luxs.
3, according to the method for the described artificially cultivating cordyceps of claim 1, it is characterized in that: the condition that described stimulation fruit body primordium produces, day and night temperature should be in 5-25 degree centigrade of scopes, and perforation is ventilated at the container closure place.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001084860A CN1152132C (en) | 2000-06-09 | 2000-06-09 | Method for artificially cultivating cordyceps |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001084860A CN1152132C (en) | 2000-06-09 | 2000-06-09 | Method for artificially cultivating cordyceps |
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| CN1274006A CN1274006A (en) | 2000-11-22 |
| CN1152132C true CN1152132C (en) | 2004-06-02 |
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| CNB001084860A Expired - Fee Related CN1152132C (en) | 2000-06-09 | 2000-06-09 | Method for artificially cultivating cordyceps |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1313595C (en) * | 2005-09-30 | 2007-05-02 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
| CN100469866C (en) * | 2005-04-30 | 2009-03-18 | 泰安市农业科学研究院 | Taishan mountain cordyceps sinensis and artificial cultivation method thereof |
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| JP2003116522A (en) * | 2001-09-28 | 2003-04-22 | Zuiei Chin | Medium for culturing cordyceps sinensis sacc. and method for culturing cordyceps sinensis sacc. |
| SG129276A1 (en) * | 2003-04-10 | 2007-02-26 | Auric Pacific Nutritech Pte Lt | Large-scale production method for cordyceps and ganoderma |
| CN1312271C (en) * | 2004-02-27 | 2007-04-25 | 俞永信 | Method of whole manual culture for chinese caterpillar fungus |
| CN100344748C (en) * | 2005-04-07 | 2007-10-24 | 雷明 | Culture medium for improving fermentation yield of aweto mycelium and method for preparing same |
| CN100386424C (en) * | 2005-12-30 | 2008-05-07 | 中国科学院微生物研究所 | Chinese caterpillar fungus cultivation method and its special-purpose culture medium |
| CN101126069B (en) * | 2007-07-05 | 2010-12-15 | 姜善清 | Cordyceps sinensis asexual propagation method |
| CN101173218B (en) * | 2007-09-30 | 2010-07-21 | 袁有宝 | Cultivate method for cordyceps militaris link |
| CN101663964B (en) * | 2009-10-09 | 2011-02-16 | 浙江天使生物工程有限公司 | Cordyceps militaris fruit body culture medium and preparation method thereof |
| CN101830753B (en) * | 2010-05-06 | 2012-09-05 | 广东省微生物研究所 | High-yield culture medium of Guangdong Cordyceps militaris fruiting body |
| CN103667170B (en) * | 2012-09-20 | 2015-10-14 | 乳源南岭好山好水冬虫夏草有限公司 | One prepares the ripe conidial method of China pilose spore |
| CN103483038B (en) * | 2013-09-05 | 2015-06-24 | 东方中科生命科学有限责任公司 | Preparation and culture method of Cordyceps culture medium, and application thereof |
| CN103704022A (en) * | 2013-12-20 | 2014-04-09 | 凌中鑫 | Method for cultivating artificial cordyceps sinensis with five-instar silkworms as carrier |
| CN105154296A (en) * | 2015-07-15 | 2015-12-16 | 十堰神农武当医药科技园有限公司 | Preparation method of cordyceps wine |
| CN105272449B (en) * | 2015-09-30 | 2017-04-05 | 鄄城县守红食用菌种植有限公司 | North Chinese caterpillar fungus culture medium and the technique for planting Cordyceps militaris (L.) Link. using the culture medium |
| CN105746172A (en) * | 2016-02-23 | 2016-07-13 | 张家港顺泰元生物科技有限公司 | Selenium-enriched cordyceps culture method |
| CN107118003A (en) * | 2017-05-20 | 2017-09-01 | 天津市东方中滨农业科技有限公司 | A kind of breeding method of Cordyceps militaris fermented bacterium and application |
| CN107810781A (en) * | 2017-11-10 | 2018-03-20 | 广西沙田仙人滩农业投资有限公司 | A kind of method that silkworm living cultivates Cordyceps militaris |
| CN110923151A (en) * | 2019-12-19 | 2020-03-27 | 广东省微生物研究所（广东省微生物分析检测中心） | Cordyceps sinensis anamorph solid culture medium and preparation method thereof |
| CN112322507A (en) * | 2020-12-09 | 2021-02-05 | 盛世荣恩生物科技有限公司 | Strain culture medium of Mortierella pseudomorpha and culture method thereof |
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2000
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100469866C (en) * | 2005-04-30 | 2009-03-18 | 泰安市农业科学研究院 | Taishan mountain cordyceps sinensis and artificial cultivation method thereof |
| CN1313595C (en) * | 2005-09-30 | 2007-05-02 | 袁有宝 | Formulation of aweto culture medium and oxygenating culture method of aweto |
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| Publication number | Publication date |
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| CN1274006A (en) | 2000-11-22 |
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