CN111972357A - Method for breeding strontium-rich hermetia illucens larvae - Google Patents
Method for breeding strontium-rich hermetia illucens larvae Download PDFInfo
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- CN111972357A CN111972357A CN202010855422.2A CN202010855422A CN111972357A CN 111972357 A CN111972357 A CN 111972357A CN 202010855422 A CN202010855422 A CN 202010855422A CN 111972357 A CN111972357 A CN 111972357A
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- strontium
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- hermetia illucens
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- 229910052712 strontium Inorganic materials 0.000 title claims abstract description 72
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 title claims abstract description 72
- 238000009395 breeding Methods 0.000 title claims abstract description 69
- 241000709785 Hermetia illucens Species 0.000 title claims abstract description 64
- 230000001488 breeding effect Effects 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000010806 kitchen waste Substances 0.000 claims abstract description 17
- 239000000463 material Substances 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims abstract description 10
- 239000003814 drug Substances 0.000 claims abstract description 10
- 239000002699 waste material Substances 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 244000144977 poultry Species 0.000 claims abstract description 8
- 239000010902 straw Substances 0.000 claims abstract description 8
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 241000894006 Bacteria Species 0.000 claims description 6
- BDAGIHXWWSANSR-NJFSPNSNSA-N hydroxyformaldehyde Chemical compound O[14CH]=O BDAGIHXWWSANSR-NJFSPNSNSA-N 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 6
- 239000004310 lactic acid Substances 0.000 claims description 6
- 229910000018 strontium carbonate Inorganic materials 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- UUCCCPNEFXQJEL-UHFFFAOYSA-L strontium dihydroxide Chemical compound [OH-].[OH-].[Sr+2] UUCCCPNEFXQJEL-UHFFFAOYSA-L 0.000 claims description 5
- 229910001866 strontium hydroxide Inorganic materials 0.000 claims description 5
- KQAGKTURZUKUCH-UHFFFAOYSA-L strontium oxalate Chemical compound [Sr+2].[O-]C(=O)C([O-])=O KQAGKTURZUKUCH-UHFFFAOYSA-L 0.000 claims description 4
- 239000000126 substance Substances 0.000 abstract description 2
- 230000019617 pupation Effects 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 229910052708 sodium Inorganic materials 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 210000003608 fece Anatomy 0.000 description 4
- 239000010871 livestock manure Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000010791 domestic waste Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention provides a method for breeding strontium-rich hermetia illucens larvae, which is characterized in that a mixed feed prepared by mixing strontium-rich materials, EM bacterial liquid and a breeding feed is used for breeding the hermetia illucens larvae, the larvae are 4-day-old larvae, the breeding feed is kitchen waste, wet waste, poultry excrement, straw, traditional Chinese medicine dregs, mushroom and the like, and strontium-rich substances are selected from one or a mixture of inorganic strontium and organic strontium.
Description
Technical Field
The invention relates to the technical field of insect breeding, in particular to a breeding method of strontium-rich hermetia illucens larvae.
Background
The hermetia illucens can effectively digest solid organic wastes such as kitchen waste, wet waste, poultry excrement, waste gas and crops, and a large amount of active ingredients such as crude protein, crude grease, mineral elements and the like are accumulated in the body, so that the hermetia illucens is an excellent protein feed for livestock and poultry and animals.
Strontium is a multifunctional life nutrient, and the functions of the strontium are mainly closely related to the formation of bones and are normal components of the bones and teeth of a human body. It is also related to the function and structure of blood vessels, and its action mechanism may be that strontium competes with sodium in the intestine for absorption sites, thereby reducing sodium absorption and increasing sodium excretion. Excessive sodium in the body is easy to cause hypertension and cardiovascular and cerebrovascular diseases, while strontium can reduce the absorption of sodium by the human body, so the strontium also has the effect of preventing diseases. Inorganic strontium has certain toxicity and cannot be directly used as a strontium source for eating. And the organic strontium has low toxicity and good palatability, and is easy to be absorbed and converted by organisms. Therefore, the development and research of organic strontium sources are very necessary.
At present, the strontium-rich breeding of hermetia illucens is not reported, and in order to further improve the application value of hermetia illucens, a breeding method of strontium-rich hermetia illucens larvae needs to be developed.
Disclosure of Invention
In view of the above, the invention provides a method for breeding strontium-rich hermetia illucens larvae.
The technical scheme of the invention is realized as follows: the invention provides a method for breeding strontium-rich hermetia illucens larvae, which comprises the following steps:
adding a strontium-rich material and EM bacterial liquid into a crushed breeding feed to prepare a mixed feed;
step two, adding the mixed feed obtained in the step one into a black soldier fly breeding pond, breeding black soldier fly larvae until the larvae enter a prepupation stage;
and step three, separating the larvae in the prepupa period and the mixed feed residues obtained in the step three to obtain strontium-rich hermetia illucens larvae and feed residues.
On the basis of the above technical scheme, preferably, in the step one, the strontium-rich material is one or a mixture of two of inorganic strontium and organic strontium.
On the basis of the technical scheme, preferably, the inorganic strontium is one or a mixture of more of strontium oxalate, strontium carbonate and strontium hydroxide.
On the basis of the technical scheme, preferably, the organic strontium is one or a mixture of two of strontium-rich lactic acid bacteria and strontium-rich yeast.
Preferably, in the step one, the breeding feed is one or a mixture of more of kitchen waste, wet waste, poultry manure, straw, traditional Chinese medicine dregs and mushroom.
On the basis of the technical scheme, preferably, in the step one, the water content of the mixed feed is 75-80%.
On the basis of the technical scheme, preferably, in the step one, the granularity of the crushed breeding feed is 60-100 meshes.
On the basis of the above technical scheme, preferably, in the step one, the strontium-rich material: the mass ratio of the breeding feed is 1: (2-12.5).
On the basis of the above technical scheme, preferably, in step two, the breeding feed: the mass ratio of the hermetia illucens larvae is 1: (0.02-0.05).
On the basis of the above technical scheme, preferably, in the second step, the hermetia illucens larvae are 4-day-old larvae.
On the basis of the technical scheme, preferably, in the second step, the temperature for breeding the hermetia illucens larvae is 30-35 ℃, and the relative humidity is 70-85%.
The preparation method of the EM bacterial liquid comprises the following steps: according to EM solid original strain: brown sugar: uniformly mixing water in a ratio of 10:100:1000, sealing, fermenting at 35-37 ℃, shaking once every 1-2 days, fermenting for 5-7 days to obtain EM liquid strain, mixing the EM liquid strain: brown sugar: mixing water at a ratio of 1:1:10, sealing, maintaining the temperature at 35-37 deg.C, shaking once every 1-2 days, and fermenting for 5-7 days to obtain EM bacterial liquid.
Compared with the prior art, the breeding method of the strontium-rich hermetia illucens larvae has the following beneficial effects:
(1) according to the invention, the feed containing strontium is added into the breeding feed, and through biotransformation in the black soldier fly insect body, toxic and side reactions and gastrointestinal stimulation of chemical strontium are eliminated, so that strontium can be absorbed more efficiently and more safely, thereby obtaining strontium-rich black soldier fly larvae and improving the economic value of the black soldier fly larvae;
(2) the breeding method is simple and easy to realize, the domestic waste and the breeding waste are used as the base materials of the breeding feed, the waste can be treated while the hermetia illucens are bred, two purposes are achieved, and the application value of the hermetia illucens is further improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Example 1
Adopting kitchen waste and wet waste as breeding feed:
respectively crushing the kitchen waste and the wet waste to obtain 60-mesh particles;
the method comprises the following steps of (1) mixing crushed kitchen waste and wet waste according to a mass ratio: 1, mixing the raw materials into a breeding feed, then adding 12.5kg of the breeding feed into every 1kg of strontium oxalate, mixing and stirring the breeding feed and the strontium oxalate, simultaneously adding EM bacterial liquid, and adjusting the water content of the obtained mixed material to 75% to obtain a mixed feed;
adding the mixed feed into a hermetia illucens breeding pond, adding 0.02kg of 4-day-old hermetia illucens larvae into every 1kg of the mixed feed, breeding at 30-35 ℃ under the condition that the relative humidity is 70-85%, and breeding until the larvae enter a pupation stage;
and screening the black soldier fly larvae and the residues of the mixed feed to obtain the strontium-rich black soldier fly larvae.
Example 2
Poultry manure and straws are adopted as breeding feed:
respectively crushing poultry manure and straws to reach the granularity of 100 meshes;
mixing the crushed poultry manure and straws according to the mass ratio of 1: 1.5, taking strontium carbonate and strontium hydroxide as strontium sources, wherein the mass ratio of the strontium carbonate to the strontium hydroxide is 1:1, then adding 2kg of cultivation feed into every 1kg of strontium sources, mixing and stirring the cultivation feed, the strontium carbonate and the strontium hydroxide, simultaneously adding EM bacterial liquid, and adjusting the water content of the obtained mixed material to 80% to obtain mixed feed;
adding the mixed feed into a hermetia illucens breeding pond, adding 0.03kg of 4-day-old hermetia illucens larvae into every 1kg of the mixed feed, breeding at 30-35 ℃ under the condition that the relative humidity is 70-85%, and breeding until the larvae enter a pupation stage;
and screening the black soldier fly larvae and the residues of the mixed feed to obtain the strontium-rich black soldier fly larvae.
Example 3
The traditional Chinese medicine dregs and the mushroom are adopted as breeding feed:
respectively crushing the traditional Chinese medicine dregs and the mushroom to reach a granularity of 80 meshes;
mixing the crushed traditional Chinese medicine dregs and the mushroom according to a mass ratio of 1:1, taking strontium-rich lactic acid bacteria as a strontium source, adding 4kg of cultivation feed into every 1kg of strontium-rich lactic acid bacteria, mixing and stirring the cultivation feed and the strontium-rich lactic acid bacteria, adding EM (effective microorganisms) bacterial liquid, and adjusting the water content of the obtained mixed material to 76% to obtain mixed feed;
adding the mixed feed into a hermetia illucens breeding pond, adding 0.04kg of 4-day-old larvae of hermetia illucens into each 1kg of the mixed feed, breeding at 30-35 ℃ under the condition that the relative humidity is 70-85%, and breeding until the larvae enter a pupation stage;
and screening the black soldier fly larvae and the residues of the mixed feed to obtain the strontium-rich black soldier fly larvae.
Example 4
Traditional Chinese medicine dregs and kitchen waste are adopted as breeding feed:
respectively crushing the traditional Chinese medicine dregs and the kitchen waste to reach a granularity of 80 meshes;
mixing the crushed traditional Chinese medicine dregs and the kitchen waste according to a mass ratio of 1: 1.5, taking strontium-rich yeast as a strontium source, adding 8kg of the breeding feed into every 1kg of the strontium-rich yeast, mixing and stirring the breeding feed and the strontium-rich yeast, adding EM (effective microorganisms) bacterial liquid, and adjusting the water content of the obtained mixed material to 77% to obtain mixed feed;
adding the mixed feed into a hermetia illucens breeding pond, adding 0.05kg of 4-day-old hermetia illucens larvae into every 1kg of the mixed feed, breeding at 30-35 ℃ under the condition that the relative humidity is 70-85%, and breeding until the larvae enter a pupation stage;
and screening the black soldier fly larvae and the residues of the mixed feed to obtain the strontium-rich black soldier fly larvae.
Example 5
Straw and kitchen waste are adopted as breeding feed:
respectively crushing straw and kitchen waste to reach a granularity of 80 meshes;
mixing the crushed traditional Chinese medicine dregs and the kitchen waste according to a mass ratio of 1:1, mixing the strontium-rich lactic acid bacteria and strontium carbonate as a strontium source, then adding 10kg of cultivation feed into every 1kg of strontium source, mixing and stirring the cultivation feed and the strontium source, simultaneously adding EM (effective microorganisms) bacterial liquid, and adjusting the water content of the obtained mixed material to 78% to obtain mixed feed;
adding the mixed feed into a hermetia illucens breeding pond, adding 0.03kg of 4-day-old hermetia illucens larvae into every 1kg of the mixed feed, breeding at 30-35 ℃ under the condition that the relative humidity is 70-85%, and breeding until the larvae enter a pupation stage;
and screening the black soldier fly larvae and the residues of the mixed feed to obtain the strontium-rich black soldier fly larvae.
Comparative example
Adopting kitchen waste as breeding feed:
adding the kitchen waste into a hermetia illucens breeding pond, adding 0.03kg of 4-day-old hermetia illucens larvae into every 1kg of kitchen waste, breeding at the temperature of 30-35 ℃ and the relative humidity of 70-85%, and breeding until the larvae enter a pupation stage;
and screening the hermetia illucens larvae and the kitchen waste to obtain the hermetia illucens larvae.
The black soldier fly larvae obtained in the example 1-5 and the comparative example are respectively dried at the temperature of 80 ℃ for 8 hours to obtain dry larvae, the dry larvae are smashed to obtain insect powder after being heavy, and the insect powder of each group is detected, wherein the detection results are as follows:
the data show that the content of strontium in the black soldier fly larvae bred by the breeding method is high, and compared with the black soldier fly larvae bred by the conventional breeding method, the content of crude protein in the black soldier fly larvae bred by the breeding method is higher, and the nutritive value is excellent.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A method for breeding strontium-rich hermetia illucens larvae is characterized by comprising the following steps:
adding a strontium-rich material and EM bacterial liquid into a crushed breeding feed to prepare a mixed feed;
step two, adding the mixed feed obtained in the step one into a black soldier fly breeding pond, breeding black soldier fly larvae until the larvae enter a prepupation stage;
and step three, separating the larvae in the prepupa period and the mixed feed residues obtained in the step three to obtain strontium-rich hermetia illucens larvae and feed residues.
2. The method for breeding strontium-rich hermetia illucens larvae according to claim 1, wherein in the first step, the strontium-rich material is one or a mixture of inorganic strontium and organic strontium.
3. The method of claim 2, wherein the inorganic strontium is selected from the group consisting of strontium oxalate, strontium carbonate, and strontium hydroxide.
4. The method of claim 2, wherein the organic strontium is one or a mixture of lactic acid bacteria rich in strontium and yeast rich in strontium.
5. The method for breeding strontium-enriched black soldier fly larvae according to claim 1, wherein in the first step, the breeding feed is one or a mixture of more of kitchen waste, wet waste, poultry excrement, straw, traditional Chinese medicine residues and mushroom.
6. The method for breeding strontium-enriched black soldier fly larvae according to claim 1, wherein in the first step, the mixed feed contains 75-80% water.
7. The method for breeding strontium-rich hermetia illucens larvae according to claim 1, wherein in step one, the strontium-rich material: the mass ratio of the breeding feed is 1: (2-12.5).
8. The method for breeding strontium-enriched hermetia illucens larvae according to claim 1, wherein in the second step, the mass ratio of the breeding feed to the hermetia illucens larvae is 1: (0.02-0.05).
9. The method of claim 1, wherein in step two, the hermetia illucens larvae are 4-day-old larvae.
10. The method of claim 1, wherein in step two, the temperature and relative humidity of the larvae of hermetia illucens are 30-35 ℃ and 70-85%.
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| CN111972357B CN111972357B (en) | 2022-05-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114586744A (en) * | 2020-12-04 | 2022-06-07 | 中国科学院分子植物科学卓越创新中心 | Method for simultaneously realizing resource treatment of kitchen waste and traditional Chinese medicine dregs through hermetia illucens |
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| CN110720435A (en) * | 2019-11-14 | 2020-01-24 | 自然创造(北京)科技有限公司 | Breeding method of hermetia illucens, selenium-rich insect protein source feed, selenium-rich organic fertilizer and processing method thereof |
| CN111084160A (en) * | 2020-01-22 | 2020-05-01 | 渑池县迈安达农业科技有限公司 | Feeding method for improving black soldier fly yield by utilizing kitchen waste and chicken manure |
-
2020
- 2020-08-24 CN CN202010855422.2A patent/CN111972357B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1156177A (en) * | 1996-02-02 | 1997-08-06 | 李江 | Artificial cultivating method for north Chinese caterpillar fungus with rich strontium |
| CN102640859A (en) * | 2012-05-17 | 2012-08-22 | 颜送贵 | Selenium strontium feed additive as well as production process and use method of selenium strontium feed additive |
| CN107716508A (en) * | 2017-09-19 | 2018-02-23 | 广东省生物资源应用研究所 | A kind of method using black soldier flies bioconversion kitchen garbage |
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| CN111084160A (en) * | 2020-01-22 | 2020-05-01 | 渑池县迈安达农业科技有限公司 | Feeding method for improving black soldier fly yield by utilizing kitchen waste and chicken manure |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114586744A (en) * | 2020-12-04 | 2022-06-07 | 中国科学院分子植物科学卓越创新中心 | Method for simultaneously realizing resource treatment of kitchen waste and traditional Chinese medicine dregs through hermetia illucens |
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