CN115606552B

CN115606552B - Breeding method of rice red ladybug

Info

Publication number: CN115606552B
Application number: CN202211636651.0A
Authority: CN
Inventors: 周霞; 郭安平; 郭运玲; 孔华; 杨小亮
Original assignee: Sanya Research Institute Chinese Academy Of Tropical Agricultural Sciences
Current assignee: Sanya Research Institute Chinese Academy Of Tropical Agricultural Sciences
Priority date: 2022-12-20
Filing date: 2022-12-20
Publication date: 2023-03-10
Anticipated expiration: 2042-12-20
Also published as: CN115606552A

Abstract

The invention provides a breeding method of rice red ladybug, belonging to the technical field of insect breeding and comprising the steps of feeding rice red ladybug by taking wild grass ears as food and increasing the feeding amount of the wild grass ears along with the increase of the age of the rice red ladybug. According to the method, the wild grass flower ears are taken as food, and the indoor feeding of the rice red ladybug is realized by matching with the setting of conditions such as a feeding device, the survival rate of the rice red ladybug is over 80 percent, the eclosion rate of adults is over 67 percent, and the egg laying amount of each female is 20-200 grains, which is equivalent to that of the rice red ladybug fed by rice pollen; the breeding method of the rice red ladybug has the advantages of high survival rate and capability of meeting the requirements of various experimental research quantities of the rice red ladybug.

Description

Breeding method of rice red ladybug

Technical Field

The invention relates to the technical field of insect breeding, in particular to a breeding method of rice red ladybug.

Background

Daihong ladybugMicraspis discolor(Fabricius) is Coleoptera, ladybug. Is distributed in many provinces in the south of China and is an important natural enemy insect in the rice field. Years of investigation find that the rice ladybug is one of the dominant species in the Hainan rice field, and predates various rice pests including thrips, rice aphids, leafhoppers, plant hoppers, lepidoptera larvae, eggs and the like. Therefore, the influence of the environmental change and cultivation system change of the rice field and various pollution factors on the rice red ladybug needs to be bred to the adult and larva of the rice red ladybug.

At present, natural prey-aphid is used as food in a method for breeding rice red ladybug, but the method needs to artificially plant a host plant for breeding the aphid, the population quantity of the aphid has natural growth and decay rules, and the sufficiency of the food cannot be ensured in the process of breeding the rice red ladybug in large quantity. Secondly, the breeding method of the host plant, namely aphid-ladybug, is greatly dependent on manpower besides the cost of a large amount of input materials and space. The drosophila melanogaster serving as the living feed for feeding the rice ladybug has the defects of low larva survival rate, prolonged development time, less final eclosion imagoes and the like. Patent document CN113749055A discloses a method for breeding rice red ladybug by using a mixed food of Mediterranean meal moth eggs and rape pollen, and the defects of high death rate and complex breeding food of rice red ladybug still exist.

Disclosure of Invention

Aiming at the problems, the invention provides a breeding method of rice red ladybug to improve the survival rate of the rice red ladybug.

In order to achieve the purpose, the technical scheme of the invention is as follows: a method for breeding rice red ladybug uses the wild grass ears as food to feed rice red ladybug, and increases the feeding amount of the wild grass ears with the increase of age of rice red ladybug.

Further, the feeding amount of the wild grass flower ears at the first age of the rice red ladybug is that 0.2g of the wild grass flower ears are fed to each rice red ladybug every day, and the feeding amount of the wild grass flower ears at each increase of the first age of the rice red ladybug at the age period is that 0.2g of the wild grass flower ears are added to each rice red ladybug every day.

Furthermore, the temperature when breeding the rice ladybug is 28 +/-1 ℃, and the photoperiod is 14 h: 10 h (L/D).

Furthermore, the container for breeding the rice ladybug is a glass tube, the moist cotton balls are added in the glass tube to keep the humidity, and the opening of the glass tube is sealed by gauze.

Further, the glass tube has a size of 1.5cm in diameter by 15cm in height.

Furthermore, one end of the rice ladybug which is primarily hatched is added into each glass tube during breeding.

Furthermore, the cotton ball is replaced every 3 days in the feeding process.

Furthermore, in the feeding process, after the adult worms emerge, male and female pairing is carried out, a pair of male and female rice ladybug are put into a glass tube, and 0.4-0.8g of wild grass ears are fed into each glass tube every day.

The method for breeding the rice red ladybug has the beneficial effects that:

the method takes the spica of the wild grass as food to feed the rice red ladybug, the vitality of the wild grass is strong, the wild grass is common in the field and can be easily obtained, the collection of the spica of the wild grass is convenient, and the requirements of the rice red ladybug on nutrient substances can be met.

Wild grass (academic name:Arundinella anomala Steudis a perennial herb of the genera gramineae, wild grass. The root and stem of the plant are thicker and stronger,dense and multi-vein scales are upright in stalk and sparse and clustered, the height can reach 110 cm, and sometimes, a plurality of sections of the scale near the ground are inclined, have adventitious roots, are hard in texture, are dark brown, and have no hairs on leaf sheaths or warts; she Sheduan with round convex upper edge and ciliated; the leaves are often hairless or only the dorsal margin is thinned to a single row of wart hairs to the entire group of brevicum hairs. The inflorescence expands or slightly contracts, the main shaft and the branch have edges, and the twin spikelet stem has no hair; the first floret is male, the top of the lemma is blunt, the anther is purple, the upper part of the lemma is slightly rough, there is no awn, and the stigma is purple red. Flowering and bearing fruit in 7-10 months.

The length of the wild grass inflorescence is 10-40 (-70) cm, the wild grass inflorescence expands or slightly contracts, the main shaft and the branch have edges, and the edges are rough or have short bristles; the twin spikelet handle is about 1.5 mm and 3 mm long respectively and has no hair; the first glume is 3-3.5 mm long and has 3-5 pulses; the second glume is 3-5 mm long and has 5 veins; the first floret is male, approximately equal to the second glume, the palea is 3-4 mm long, the top end is blunt, 5 veins are formed, the anther is purple, and the length is 1.6 mm; the second small flower is 2.8-3.5 mm long, the upper part of the palea is rough, 3-5 veins are not obvious, no awn exists, and the palea sometimes has a small awn tip of 0.6-1 mm; the length of the base plate hair is 1-1.3 mm, which is about 1/2 of the palea body; the stigma is purple red.

The method takes the wild grass ears as food to feed the rice red ladybug, and accords with the feeding habit of the rice red ladybug. According to the method, the wild grass flower ears are taken as food, and the indoor breeding of the rice red ladybug is realized by matching with the arrangement of conditions such as a breeding device, the survival rate of the rice red ladybug is over 80 percent, and the eclosion rate of adults is over 67 percent; the breeding method of the rice red ladybug has the advantages of high survival rate and capability of meeting the requirements of various experimental research quantities of the rice red ladybug.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.

The invention relates to a breeding method of rice red ladybug, which comprises the steps of firstly preparing a plurality of clean glass tubes (the diameter is 1.5cm multiplied by the height is 15.0 cm), adding one end of rice red ladybug which is primarily hatched into each tube (the rice red ladybug is grabbed from a rice field and is obtained after spawning and hatching), adding a wet cotton ball into each tube to keep humidity, and sealing the opening of each tube by using a rubber band to hoop gauze. Adding wild grass Arundinella anomala Stend flower spike as food, picking up the wild grass flower spike (one flower spike with 10-20 small branches) which is not completely opened from bracts between 6-8 am, putting into a plastic sealing bag, feeding adult rice red ladybug and larva, adding 0.2g (5 small branches of one flower spike) feed every day when the larva is old, and increasing the amount of food fed every day (5 small branches when the larva is old). The vast majority of rice ladybug larvae are 4 years old, and the minority are 3 years old. The rice ladybug eats the tender part of the wild grass flower ear. The food left by the utility model is cleaned every day. The cotton ball is changed once in 3 days to prevent mildew and pollution. And carrying out male-female pairing after the imagoes emerge. A pair of adult males and females were placed in a glass tube (diameter 1.5cm X height 15.0 cm) and fed with one spike of wild grass per glass tube per day. The female insects lay eggs on the wall of the glass tube, the glass tube is replaced every day after laying eggs, and eggs attached to the replaced glass tube are used for continuous hatching and utilization. The above-mentioned breeding environment was carried out in a climatic chamber at (28. + -. 1) ℃ and a photoperiod of 14 h: 10 h (L/D).

Example 1

In this example, 100 glass tubes of the above size were prepared, and the breeding environment was a climatic chamber at (28 ± 1) ° c and a light period of 14 h: 10 h (L/D), 1 st age of rice red ladybug was placed in each glass tube, and the ears of wild grass flowers were collected and fed to rice red ladybug as food, and 0.2g (5 small branches of the ears of wild grass) of each one age of rice red ladybug was fed to each one day; each of the rice red ladybugs increases 0.2g of wild grass ears every day when the rice red ladybugs are increased by one year; the survival number of the rice ladybug before eclosion is 87, and the survival number of the rice ladybug which can successfully complete eclosion is 72.

Example 2

In this example, 100 glass tubes of the above size were prepared and fed in a climatic chamber at 28. + -. 1 ℃ C. And a photoperiod of 14 h: 10 h (L/D), the emerged rice red ladybug of example 1 was divided into males and females, and a pair of adult males and females were placed in one glass tube (diameter 1.5cm X height 15.0 cm), and one spike of wild grass was fed to each glass tube every day. In the embodiment, 100 pairs of male and female rice ladybug are selected for breeding, the female rice ladybug lay eggs on the wall of the glass tube, the glass tube is replaced every day after laying eggs, and eggs attached to the replaced glass tube are used for continuous hatching and utilization. The egg laying amount per female is 20-200 grains, and the average egg laying amount is 148 grains.

Example 3

In this example, 100 glass tubes of the above size were prepared, and the breeding environment was (28 ± 1) ° c and 14 h: 10 h (L/D) photoperiod in an artificial climate room, the rice red ladybirds hatched in example 2 were further bred, 1 first age of rice red ladybirds were placed in each glass tube, the wild grass spica was collected, the wild grass spica was fed to rice red ladybirds as food, and 0.2g (5 small branches of the wild grass spica) of each rice red ladybird at one age was fed daily; each of the rice red ladybugs increases 0.2g of wild grass ears every day when the rice red ladybugs are increased by one year; the survival number of the rice ladybug before eclosion is 84, and the survival number of the rice ladybug which can successfully complete eclosion is 67.

Experiments prove that the survival rate of the rice red ladybug bred by the method is over 80 percent, the eclosion rate of adults is over 67 percent, and the egg laying amount of each female is 20-200 grains (which is equivalent to that of the rice red ladybug fed by rice pollen). The breeding method of the rice red ladybug has the advantages of high survival rate and capability of meeting the requirements of various experimental research quantities of the rice red ladybug.

Comparative example 1

The comparative example is a comparison experiment of example 1, and is different from example 1 in that the food for raising the rice red ladybug is aphid, the survival rate of the rice red ladybug fed by the aphid before eclosion of imago is 76%, the eclosion rate of the imago is 64%, and the survival rate is lower than that of the rice red ladybug in example 1 and the eclosion rate of the imago, meanwhile, the rice red ladybug is fed by the aphid as the food, a host plant for breeding the aphid needs to be artificially planted, the population number of the aphid has a natural growth rule, and the sufficiency of the food cannot be ensured in the process of breeding the rice red ladybug in a large scale.

Comparative example 2

The comparative example is a comparative experiment of example 1, and is different from example 1 in that rice pollen is used for feeding rice red ladybug, and the survival rate of the rice red ladybug fed with rice pollen as food before eclosion of imago is 82% and the eclosion rate of the imago is 67%, which is equivalent to that of the rice red ladybug in example 1 and the eclosion rate of the imago, but the collection of the rice pollen is complicated, so that buds which are not opened in the rice need to be collected, and then the buds are dissected to collect the pollen therein, so that the rice red ladybug is not suitable for large-scale cultivation.

Comparative example 3

This comparative example is a comparative experiment of example 2, except that rice pollen was used to feed rice ladybug, adult rice was taken from the rice ladybug bred with rice pollen in comparative example 2, and other experiments were the same as in example 1, and it was found that the egg laying amount per female was between 30 and 160, and the average egg laying amount was 117.

It should be apparent that the described embodiments are only some of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

Claims

1. A breeding method of rice red ladybug is characterized in that the rice red ladybug is fed with the wild grass flower ears as food, and the feeding amount of the wild grass flower ears is increased along with the increase of the age of the rice red ladybug.

2. The raising method for rice red ladybug as claimed in claim 1, wherein the amount of the wild grass ears fed per rice red ladybug at the age of one year is 0.2g of the wild grass ears per day per ladybug, and the amount of the wild grass ears fed per age of one year at the age of one year is 0.2g of the wild grass ears per day per ladybug.

3. The method for raising rice red ladybug as claimed in claim 1, wherein the temperature for raising rice red ladybug is 28 ± 1 ℃ and the photoperiod L/D is 14 h: 10 h.

4. The breeding method of rice red ladybug as claimed in any one of claims 1 to 3, wherein the container for breeding rice red ladybug is a glass tube, the moistened cotton ball is kept wet in the glass tube, and the mouth of the glass tube is sealed with gauze.

5. The raising method of rice red ladybug as claimed in claim 4, wherein the size of the glass tube is 1.5cm in diameter and 15cm in height.

6. The breeding method of rice red ladybug as claimed in claim 4, wherein a single head of rice red ladybug which had been hatched initially is added to each glass tube.

7. The raising method of rice red ladybug as claimed in claim 4, wherein the cotton balls are replaced every 3 days during the raising.

8. The breeding method of rice red ladybug as claimed in claim 4, wherein in the breeding process, after eclosion of adult rice, male and female pairing is performed, a pair of male and female rice red ladybug are placed in a glass tube, and 0.4-0.8g of wild grass ears are fed into each glass tube every day.