CN115606552B - A kind of raising method of rice red ladybug - Google Patents

Abstract

The invention provides a method for raising red ladybugs, which belongs to the technical field of insect breeding, and comprises feeding the red ladybugs with wild ancient grass flower spikes as food, and increasing the wild ancient grass flower spikes with the increase of the age of the red ladybugs feeding amount. The present invention uses wild ancient grass flower spikes as food, cooperates with the setting of the feeding device and other conditions, so that the rice red ladybug can be reared indoors, and the survival rate of the rice red ladybug is more than 80%, and the eclosion rate of adults is more than 67%. The amount of eggs laid is between 20-200 grains, which is equivalent to the amount of eggs laid by the red ladybug fed with rice pollen; the breeding method of the red ladybug of the present invention has a high survival rate and can satisfy various experiments of the red ladybug. Study the merits of quantity requirements.

Description

A kind of raising method of rice red ladybug

technical field

The invention relates to the technical field of insect breeding, in particular to a method for raising the red ladybug.

Background technique

The rice red ladybug Micraspis discolor (Fabricius) belongs to the Coleoptera, Coccinidae. Distributed in many provinces in southern my country, it is an important natural enemy insect in rice fields. Years of investigations have found that the red ladybug is one of the dominant species in rice fields in Hainan, preying on a variety of rice pests including thrips, rice aphids, leafhoppers, planthoppers, Lepidoptera larvae and eggs. Therefore, it is necessary to raise the adults and larvae of the red lady beetle in order to study the effects of changes in the paddy field environment and farming system, as well as various pollution factors on the beetle.

At present, the method of cultivating red ladybugs uses natural prey-aphids as food, but this method requires artificial planting of host plants for the reproduction of aphids, and the population of aphids has a natural law of ebb and flow. In the process of mass breeding of red ladybugs Adequacy of food cannot be guaranteed. Secondly, using the feeding method of host plants-aphids-ladybugs, in addition to the need to invest a lot of materials and space costs, it also relies heavily on labor. Using Drosophila as live feed to feed the rice red ladybug has disadvantages such as low larval survival rate, prolonged development time, and few final eclosion adults. Patent document CN113749055A discloses a method for raising red ladybugs by using mixed food of mealworm eggs and rape pollen, but there are still defects of high mortality of red ladybugs and complicated food.

Contents of the invention

In view of the above problems, the present invention provides a method for raising the red ladybug, so as to improve the survival rate of the red ladybug.

In order to achieve the above object, the technical solution of the present invention is: a method for raising the red ladybug, feeding the red ladybug with the flower spikes of wild ancient grass, and increasing the number of red ladybugs with the increase of the age of the red ladybug. Feeding amount of grass spikes.

Further, the feeding amount of wild ancient grass flower spikes during the first instar rice red ladybug is that each rice red ladybug feeds 0.2g wild ancient grass flower spikes every day, and the rice red ladybug increases the number of wild ancient grass flower spikes for every one age. The feeding amount is to increase 0.2g wild ancient grass flower spike every day for each rice red ladybug.

Further, the temperature for rearing the red ladybug is 28±1°C, and the photoperiod is 14h:10h (L/D).

Further, the container used for raising the rice red ladybug is a glass tube, and moistened cotton balls are added in the glass tube to maintain humidity, and the mouth of the glass tube is sealed with gauze.

Further, the size of the glass tube is 1.5 cm in diameter x 15 cm in height.

Further, a newly hatched red ladybug was added to each glass tube when raising.

Further, the cotton balls were replaced every 3 days during the raising process.

Further, during the feeding process, after the adults emerge, the male and female pairs are performed, and a pair of male and female red ladybugs are put into a glass tube, and 0.4-0.8 g of wild ancient grass spikes are fed to each glass tube every day.

The beneficial effects of the rice red ladybug breeding method of the present invention are:

The present invention uses the flower spikes of the wild ancient grass as food to feed the rice red ladybug. The wild ancient grass has tenacious vitality, is common in the field and can be easily obtained. need.

Arundinella anomala (scientific name: Arundinella anomala Steud .) is a perennial herbaceous plant belonging to Poaceae and Arundinella anomala. The rhizome is relatively strong, densely grown with multi-veined scales, the culms are erect, sparsely clustered, up to 110 cm high, sometimes several nodes near the ground are inclined and have adventitious roots, hard, the nodes are dark brown, the leaf sheaths are glabrous or warty; ligule Short, with rounded upper margin, ciliate; leaf blade usually glabrous or only a row of sparsely warty hairs on the back edge to all covered with short warty hairs. Inflorescences spread or slightly contracted, main axis and branches ridged, twin spikelet stalks glabrous respectively; first floret male, lemma apex blunt, anthers purple, lemma upper slightly scabrous, awnless, stigma purple-red. Flowering and fruiting from July to October.

Wild ancient grass inflorescence 10-40(-70) cm long, expanding or slightly contracted, main axis and branches with ribs, rough or short bristles on ribs; twin spikelets about 1.5 mm and 3 mm long, glabrous ; first glume 3-3.5 mm long, with 3-5 veins; second glume 3-5 mm long, with 5 veins; first floret male, about as long as second glume, lemma 3-4 mm long, apex Blunt, 5-veined, anthers purple, 1.6 mm long; second floret 2.8-3.5 mm long, lemma slightly rough on upper part, 3-5 veins inconspicuous, no awn, sometimes with 0.6-1 mm awn-like tip; Basal disc hairs 1-1.3mm long, about 1/2 of lemma body; stigma purplish red.

The present invention uses the wild ancient grass flower spikes as food to raise the rice red ladybug, which conforms to the feeding habit of the rice red ladybug. The present invention uses wild ancient grass flower spikes as food, cooperates with the setting of the feeding device and other conditions, so that the rice red ladybug can be reared indoors, and the survival rate of the rice red ladybug is more than 80%, and the adult eclosion rate is more than 67%; the present invention The breeding method of the rice red ladybug has the advantages of high survival rate and can meet the quantity requirements of various experimental researches of the rice red ladybug.

Detailed ways

The technical solutions in the embodiments of the present invention are clearly and completely described below. In the following description, a lot of specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, and those skilled in the art can do it without departing from the meaning of the present invention. By analogy, the present invention is therefore not limited to the specific examples disclosed below.

The raising method of a kind of rice red ladybug of the present invention, at first is to prepare a plurality of clean glass tubes (diameter 1.5cm * high 15.0 cm), every tube adds a newly hatched rice red ladybug (catch from paddy field) Rice red ladybug adults, obtained after laying eggs and hatching), add a wet cotton ball in the tube to maintain humidity, and seal the tube mouth with gauze with a rubber band. Add the wild ancient grass Arundinella anomala Stend flower spikes as food, pick the wild ancient grass flower spikes that have not fully opened from the bracts (one flower spike 10-20 small branches) between 6-8 o'clock in the morning, and put them into plastic airtight bags In the middle, feed the adults and larvae of the red ladybug, add 0.2g (5 small branches of a flower spike) feed every day at the first instar, and increase the amount of food fed every day as the larvae age increases (increase for each additional instar) 5 small branches). Most of the larvae of the red ladybug are 4th instar, and a few are 3rd instar. The rice red ladybug feeds on the young part of the flower spikes of wild ancient grass. Clean up its leftovers in time every day. Change the cotton balls every 3 days to prevent mildew and pollution. Male and female pairing takes place after adult eclosion. A pair of male and female adults were put into a glass tube (diameter 1.5 cm×height 15.0 cm), and each glass tube was fed with one flower panicle of E. japonica every day. The female lays eggs on the wall of the glass tube, and the glass tube is replaced every day after laying eggs, and the eggs attached to the replaced glass tube are used for continued hatching and utilization. The above-mentioned breeding environment was carried out in an artificial climate room at (28 ± 1 ) ℃ and 14h: 10 h (L/D) photoperiod.

Example 1

In this example, 100 glass tubes of the above-mentioned size were prepared, and the breeding environment was (28 ± 1 ) ℃ and an artificial climate room with a 14h: 10 h (L/D) photoperiod, and one first-year-old Daohong was placed in each glass tube. Ladybugs, collect wild ancient grass flower spikes, and use wild ancient grass flower spikes as food to feed rice red ladybugs, each of the first instar rice red ladybugs is fed with 0.2g (5 small branches of wild ancient grass flower spikes) per day Ancient grass flower spikes; Daohong ladybugs increase 0.2g of wild ancient grass flower spikes per day for each additional instar; the number of Daohong ladybugs survived before eclosion was 87, and 72 were able to successfully complete eclosion.

Example 2

In this embodiment, 100 glass tubes of the above-mentioned size are prepared, and the breeding environment is (28 ± 1 ) ℃ and an artificial climate room with a 14h: 10 h (L/D) photoperiod, and the rice red ladybug after eclosion in Example 1 Separate males and females, put a pair of male and female adults into a glass tube (1.5 cm in diameter x 15.0 cm in height), and feed one flower spike of Wild ancient grass in each glass tube every day. In the present embodiment, 100 pairs of male and female rice red ladybugs are selected to raise, and the females lay eggs on the glass tube wall. After laying eggs, the glass tubes are replaced every day, and the eggs attached to the changed glass tubes are used for continued hatching and utilization. The number of eggs laid by each female is between 20-200, and the average number of eggs laid is 148.

Example 3

This embodiment prepares 100 glass tubes of the above-mentioned size, and the raising environment is (28 ± 1 ) ℃ and 14h: 10 h (L/D) photoperiod in an artificial climate room, and the rice red ladybug hatched in Example 2 is continued. For raising, place 1 first-year-old red ladybug in each glass tube, collect wild ancient grass flower spikes, and use wild ancient grass flower spikes as food to feed rice-red ladybugs, and the first-year-old rice-red ladybugs are fed every day Feed 0.2g (5 small branches of wild ancient grass flower spikes) wild ancient grass flower spikes; rice red ladybugs add 0.2g wild ancient grass flower spikes per day for each additional instar; the number of rice red ladybugs before eclosion is 84, There were 67 heads that successfully completed eclosion.

It has been verified by experiments that the survival rate of the red ladybug raised by this method of the present invention is more than 80%, the eclosion rate of adults is more than 67%, and the amount of eggs laid by each female is between 20-200 grains (compared to that of rice pollen-fed). Rice red ladybug quite). The breeding method of the red ladybug of the invention has the advantages of high survival rate and can meet the quantity requirements of various experimental researches of the red ladybug.

Comparative example 1

This comparative example is the comparative experiment of embodiment 1, and the difference with embodiment 1 is that the food of raising rice red ladybug is aphids, and others are the same as embodiment 1, and the rice red ladybug fed by aphids is food before adult emergence The survival rate is 76%, the adult eclosion rate is 64%, compared with the survival rate and the adult eclosion rate of the red ladybug in embodiment 1, it is low, and the rice red ladybug needs to be artificially planted for the aphids to breed simultaneously with aphids as food feeding host plants, and the population of aphids has a natural growth and decline rule, and the adequacy of food cannot be guaranteed in the process of mass breeding of red ladybug.

Comparative example 2

This comparative example is the comparative experiment of embodiment 1, and the difference is that the rice red ladybug is fed with rice pollen, and others are the same as in Example 1. The survival rate of the rice red ladybug fed with rice pollen before adult emergence is 82%, and the adult eclosion rate is 67%, which is equivalent to the survival rate and adult eclosion rate of the red ladybug in Example 1, but the collection of rice pollen is relatively loaded down with trivial details, and it is necessary to collect unopened flower buds of rice, which are then dissected and collected. Pollen is not suitable for large-scale farming.

Comparative example 3

This comparative example is the comparative experiment of embodiment 2, and difference is to use rice pollen to feed rice red ladybug, and adult is taken from the rice red ladybug that comparative example 2 raises with rice pollen, other is the same as embodiment 1, through statistics, each The amount of eggs laid by a female is between 30-160, and the average amount of eggs laid is 117.

Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

Claims (8)

1. A breeding method of rice red ladybug is characterized in that the rice red ladybug is fed with the wild grass flower ears as food, and the feeding amount of the wild grass flower ears is increased along with the increase of the age of the rice red ladybug.

2. The raising method for rice red ladybug as claimed in claim 1, wherein the amount of the wild grass ears fed per rice red ladybug at the age of one year is 0.2g of the wild grass ears per day per ladybug, and the amount of the wild grass ears fed per age of one year at the age of one year is 0.2g of the wild grass ears per day per ladybug.

3. The method for raising rice red ladybug as claimed in claim 1, wherein the temperature for raising rice red ladybug is 28 ± 1 ℃ and the photoperiod L/D is 14 h: 10 h.

4. The breeding method of rice red ladybug as claimed in any one of claims 1 to 3, wherein the container for breeding rice red ladybug is a glass tube, the moistened cotton ball is kept wet in the glass tube, and the mouth of the glass tube is sealed with gauze.

5. The raising method of rice red ladybug as claimed in claim 4, wherein the size of the glass tube is 1.5cm in diameter and 15cm in height.

6. The breeding method of rice red ladybug as claimed in claim 4, wherein a single head of rice red ladybug which had been hatched initially is added to each glass tube.

7. The raising method of rice red ladybug as claimed in claim 4, wherein the cotton balls are replaced every 3 days during the raising.

8. The breeding method of rice red ladybug as claimed in claim 4, wherein in the breeding process, after eclosion of adult rice, male and female pairing is performed, a pair of male and female rice red ladybug are placed in a glass tube, and 0.4-0.8g of wild grass ears are fed into each glass tube every day.