CN115554207A - Plant composition and preparation method thereof - Google Patents

Plant composition and preparation method thereof Download PDF

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Publication number
CN115554207A
CN115554207A CN202211372715.0A CN202211372715A CN115554207A CN 115554207 A CN115554207 A CN 115554207A CN 202211372715 A CN202211372715 A CN 202211372715A CN 115554207 A CN115554207 A CN 115554207A
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extract
fruit
elaeagnus
herba
water
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CN115554207B (en
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招敏聪
林霁
王维龙
贺锐
解勇
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Beijing Plant Doctor Biotechnology Co ltd
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Beijing Plant Doctor Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/08Anti-ageing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/20Chemical, physico-chemical or functional or structural properties of the composition as a whole
    • A61K2800/30Characterized by the absence of a particular group of ingredients
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention belongs to the field of plant products, and provides a plant composition and a preparation method thereof. According to the invention, a large number of researches show that the extract has good antioxidant effect and moisturizing effect; the skin care cream is not added with various substances which are easy to be allergic, such as pigments, alcohol and the like, and is mild and non-irritant to skin; can be added into resident cosmetics, and has good popularization and application prospect.

Description

Plant composition and preparation method thereof
Technical Field
The invention belongs to the field of cosmetics, and particularly relates to a plant composition and a preparation method thereof.
Background
The genus Cornus (Cornus) is a Cornaceae (Cornaceae) plant, and is named because there are two pairs of symmetrical petal-shaped large bracts outside the inflorescence. The tree shape of the four-flower tree is beautiful, and the trees are full of flowers when flowers are bloomed; the autumn is the fruit ripening period, the fruit is red when ripe, and the fruit is remained on the tree for more than 1 month.
China is one of the original producing areas of the four-candlelight; the fruit is edible; the Chinese medicine can be used for treating dysentery, traumatic hemorrhage, fracture, etc. recorded in Huashan Yao Zhi (records of Huashan Yao Zhi); at present, china is very rare in the application and development of the cornus dahliae in the fine chemical industry, and only CN 109771350A, namely a skin barrier repair composition, essence and a preparation method thereof, disclose that the cornus dahliae extract, the fig extract and the poncirus trifoliate extract can be used for repairing the skin of a human body; however, the patent does not describe the extract of the four-flower buds any more, and the parts, extraction methods and specific functions used in the method are not further studied.
The Elaeagnus pungens fruit is native plant in China, belonging to the family Elaeagnaceae; also named as elaeagnus conferta roxb, radix aconiti kusnezoffii, fructus junci, etc.; widely distributed in the south of the Yangtze river in China; is a native wild fruit with low popularity, and has the effects of astringing to stop diarrhea and stopping dysentery. It is commonly used for enteritis, diarrhea and dysentery. At present, most of the development aiming at the elaeagnus pungens is concentrated on oral administration; in the field of fine chemistry, topical skin compositions such as CN108403521A and uses thereof are mentioned only for lightening melanin pigmentation of human skin.
Therefore, it is necessary to comprehensively develop a large number of native plant resources in our country.
Disclosure of Invention
The invention provides a plant composition and a preparation method thereof, aiming at the current development situation of native plants in China.
The plant composition is characterized by comprising the following components in parts by mass:
Figure BDA0003925707300000011
Figure BDA0003925707300000021
tetrahydro-methyl-pyrimidine-carboxylic acid 0.3-0.5;
the herba Zeylanicae Quillatae fruit extract is water extract of peeled herba Zeylanicae Quillatae fruit; the elaeagnus pungens fruit extract is a water extract of elaeagnus pungens fruit.
Particularly, the preparation method of the camellia nitidissima extract comprises the following steps: peeling ripe fruit of herba Zebrinae Pendulae; adding 5-8 times of water at 0-8 deg.C for extracting, filtering to obtain solution, and evaporating at low temperature to constant weight to obtain herba Senecionis Chrysanthemoidis extract.
Further on; the preparation method of the candlenut extract comprises the following steps:
(1) Peeling ripe herba Zeylanicae Makino fruit, and crushing;
(2) Adding reflux water with volume of 5-8 times at 0-8 deg.C, extracting for 8-12 hr with reflux frequency of 3;
(3) Filtering to obtain solution, and evaporating at 40 deg.C or below to constant weight to obtain herba Tetrastigmatis Hypoglauci extract.
In particular, the mature elaeagnus pungens fruit is denucleated; adding 5-8 times of water at 0-8 deg.C for extracting, filtering to obtain solution, and evaporating at low temperature to constant weight to obtain Elaeagnus glabra fruit extract.
Further on; the preparation method of the elaeagnus pungens fruit extract comprises the following steps:
(1) Cleaning mature Elaeagnus glabra fruit, crushing, and removing core;
(2) Adding 5-8 times of reflux water at 0-8 deg.C for extraction for 8-12h, reflux frequency being 3;
(3) Filtering the extractive solution, and evaporating at a temperature of less than or equal to 40 deg.C to constant weight to obtain Elaeagnus glabra fruit extract.
Particularly, the preparation method of the pansy water comprises the following steps: cleaning whole pansy, crushing, and squeezing.
The invention also discloses an application of the plant composition in resident cosmetics.
The invention has the beneficial effects that:
the plant composition prepared by the invention is mainly three native plants in China, and the eating history is long, but no further development is carried out; according to the invention, a large number of researches prove that the water extract has good antioxidant effect and moisturizing effect; the skin care product is not added with various substances which are easy to be allergic, such as pigments, alcohol and the like, and is mild and non-irritant to the skin; can be added into resident cosmetics and has good popularization and application prospect.
Detailed Description
The present invention will be further illustrated in detail with reference to the following specific examples, which are not intended to limit the present invention but are merely illustrative thereof. The experimental methods used in the following examples, unless otherwise specified, and experimental methods not specified in specific conditions in the examples, are generally commercially available according to conventional conditions, and materials, reagents, and the like used in the following examples, unless otherwise specified.
In the present invention, the common daylily is a plant (Dendrobenthamia gigantea) of the genus common daylily of the family Cornaceae;
the fructus Elaeagni refers to plant of Elaeagnus genus of Elaeagnaceae family (Elaeagnus glabra Thunb.);
the pansy is a Viola plant (Viola tricolor L.) belonging to Violaceae.
The preparation method of the yacon extract comprises the following steps:
(1) Peeling ripe herba Zebrinae Pendulae fruit, and crushing;
(2) Adding water at low temperature, reflux extracting for 3 times;
(3) Filtering to obtain solution, and evaporating at 40 deg.C or below to constant weight to obtain herba Tetrastigmatis Hypoglauci extract; and (5) standby.
The technical parameters of each step are shown in table 1.
TABLE 1
Figure BDA0003925707300000031
The preparation method of the elaeagnus pungens fruit extract comprises the following steps:
(1) Cleaning mature elaeagnus pungens fruit, removing impurities attached to the surface, cutting the fruit, removing the core, and crushing the peel;
(2) Adding water at low temperature, reflux extracting for 3 times;
(3) Filtering the extractive solution, evaporating at 40 deg.C or below to constant weight to obtain Elaeagnus glabra fruit extract; and (4) standby.
The technical parameters of each step are shown in table 2.
TABLE 2
Figure BDA0003925707300000032
The preparation method of pansy water comprises the following steps:
(1) Selecting pansy in flowering period;
(2) Sucking the attached soil and other impurities, and drying the surface;
(3) Crushing and squeezing the pansy, and filtering to remove insoluble substances to obtain pansy water.
The preparation method of the plant composition comprises the following steps:
the prepared dogwood fruit extract, elaeagnus pungens fruit extract, pansy water and the rest raw materials were mixed to be completely dissolved as shown in table 3 below.
TABLE 3 addition amount (parts by mass)
Serial number Example 1 Example 2 Example 3 Example 4 Example 5 Example 6
Sequence number of the extract of Dioscorea Opposita 1 1 2 2 3 3
Adding amount of herba Zebrinae Pendulae extract 10 15 10 15 10 15
Sequence number of Elaeagnus pungens fruit extract 1 1 2 2 3 3
Adding amount of fructus Elaeagni extract 8 5 6 6 6 6
Adding amount of pansy water 30 36 36 36 36 36
Red myrrhAlcohol addition amount 1 1 1 1 1.3 1.3
Tetrahydrophylpyrimidine carboxylic acid addition amount 0.5 0.5 0.3 0.3 0.5 0.5
Human skin patch test
The 6 groups of plant compositions obtained are referred to the human skin patch test in 2022 cosmetic safety technical Specification.
The skin reactions were observed at 30min (after disappearance of the indentation), 24h and 48h after removal of the mixed fermentation containing plants, respectively, according to the standard and the observation results were recorded, see table 4.
TABLE 4 human body safety test results
Numbering 30min 24h 48h
Example 1 Grade 0, 30 persons Grade 0, 30 persons Grade 0, 30 people
Example 2 Grade 0, 30 people Grade 0, 30 persons Grade 0, 30 persons
Example 3 Grade 0, 30 persons Grade 0, 30 persons Grade 0, 30 people
Example 4 Grade 0, 30 persons Grade 0, 30 persons Grade 0, 30 persons
Example 5 Grade 0, 30 persons Grade 0, 30 persons Grade 0, 30 persons
Example 6 Grade 0, 30 people Grade 0, 30 people Grade 0, 30 persons
Sample stability testing
(1) Stability of acceleration
Taking the six prepared examples, taking three in each example, placing a proper amount of the three prepared examples in a centrifuge tube, sealing the tube opening, and centrifuging at 3000rpm for 30min; visually observing the shape; no precipitation or precipitation was observed, and the original appearance was maintained.
(2) Stability at varying temperature
1. And (3) heat resistance test: adjusting the constant temperature incubator to 40 ℃, putting three of the prepared six examples in a transparent glass bottle with the sample loading amount of 20 ml/bottle, sealing, putting in the constant temperature incubator for three months, taking out, returning to the room temperature, and observing the appearance change.
2. Cold resistance test: adjusting the temperature of the constant temperature incubator to-10 ℃, putting three samples of the prepared six examples in a transparent glass bottle with the sample amount of 20 ml/bottle, sealing, putting the bottle in the constant temperature incubator for three months, taking out the bottle, returning to the room temperature, and observing the appearance change.
3. And (3) normal temperature testing: three of the six prepared examples are filled in transparent glass bottles, the sample amount is 20 ml/bottle, the bottles are placed at normal temperature for 6 months after being sealed, and the appearance change of the essence is observed.
No phenomena such as precipitation and precipitation are observed in the heat resistance test, the cold resistance test and the normal temperature test, and the original appearance is kept.
Skin Water loss test
Testing with Trans Epidermal Water Loss (TEWL); the test is carried out by adopting a TM300 tester of Courage & Khazaka company in Germany, and the specific steps are as follows:
each group of examples 5 women without skin disease aged 20 to 40 years were randomly selected; the test specimens (0.5 ml) of the examples, diluted 10-fold with deionized water twice a day in the morning and at night, were applied evenly to the inner forearm, and then the same tester was used to test the transepidermal water loss at week 0 and after continued use to week 2 and week 4, respectively; specific detection results; the results are shown in Table 5.
The rate of decrease of the TEWL value is formulated as:
TEWL value decrease rate (%) = (A0-An)/A0X 100%
Wherein A0 is the week 0 TEWL value and An is the week n TEWL value.
Mean rate of decrease (%) in TEWL level (= sum of rate of decrease in TEWL level per group/number of persons in the group
Table 5 skin moisture loss test results
Figure BDA0003925707300000051
Oxidation resistance test
The plant composition prepared in each example was subjected to an antioxidant test for DPPH free radical scavenging; the specific method comprises the following steps:
experimental materials: diphenylpicrylphenylhydrazine radical (abbreviation: DPPH, sigma, USA);
du800 uv-vis spectrophotometer (Beckman corporation, usa);
the test objects total 6 groups, and the sample solution is the plant composition prepared in the above examples and diluted 10 times (i.e. the content is 10%) with deionized water.
The experimental method comprises the following steps: taking 0.1mL of sample solution, adding 2mL of 60 mu mol/L DPPH solution, uniformly mixing, standing for 30min, adjusting the zero point by using an original solvent, and measuring the absorbance at 517nm to be Ai; uniformly mixing 0.1mL of absolute ethyl alcohol solvent and 2mL of DPPH solution by the same method, and determining the absorbance as Ac;0.1mL of the sample solution was mixed with 2mL of an absolute ethanol solvent and the absorbance was measured as Aj. The free radical clearance rate is calculated according to the following formula; the results are shown in Table 6.
Clearance (%) = [1- (Ai-Aj)/Ac ]. Times.100%
Wherein Aj reflects the contribution of the sample itself to absorbance; absorbance values of Ai samples after action on DPPH; ac is the absorbance of DPPH itself at the measurement wavelength.
TABLE 6 antioxidant assay results
Number of Clearance rate (a)%)
Example 1 (dilution 10 times) 93.2
Example 2 (dilution 10 times) 94.6
Example 3 (dilution 10 times) 92.8
Example 4 (dilution 10 times) 94.1
Example 5 (dilution 10 times) 93.4
Example 6 (dilution 10 times) 94.2
The in vitro experiment results show that: the invention has better free radical scavenging ability.

Claims (7)

1. The plant composition is characterized by comprising the following components in parts by mass:
Figure FDA0003925707290000011
tetrahydro-methyl-pyrimidine-carboxylic acid 0.3-0.5;
the herba Zeylanicae Quillatae fruit extract is water extract of peeled herba Zeylanicae Quillatae fruit; the elaeagnus pungens fruit extract is an aqueous extract of elaeagnus pungens fruit.
2. The botanical composition as claimed in claim 1, wherein the extract of the dendrobenthamia extract is prepared by: peeling ripe fruit of herba Zebrinae Pendulae; adding 5-8 times of water at 0-8 deg.C for extracting, filtering to obtain solution, and evaporating at low temperature to constant weight to obtain herba Senecionis Chrysanthemoidis extract.
3. The botanical composition of claim 2, wherein the extract of Dioscorea alata is prepared by:
(1) Peeling mature fruit of herba Zebrinae Pendulae, and crushing;
(2) Adding reflux water with volume of 5-8 times at 0-8 deg.C, extracting for 8-12 hr with reflux frequency of 3;
(3) Filtering the extractive solution, and evaporating at a temperature of less than or equal to 40 deg.C to constant weight to obtain herba Hyperici Erecti extract.
4. The plant composition according to claim 1, wherein the elaeagnus pungens fruit extract is prepared by: removing kernels from mature elaeagnus pungens fruits; adding 5-8 times of water at 0-8 deg.C for extracting, filtering to obtain solution, and evaporating at low temperature to constant weight to obtain Elaeagnus glabra fruit extract.
5. The plant composition according to claim 4, wherein the Elaeagnus glabra fruit extract is prepared by the following steps:
(1) Cleaning mature Elaeagnus glabra fruit, crushing, and removing core;
(2) Adding 5-8 times of reflux water at 0-8 deg.C for extraction for 8-12h, reflux frequency being 3;
(3) Extracting, filtering to obtain solution, and evaporating at a temperature of less than or equal to 40 deg.C to constant weight to obtain Elaeagnus glabra fruit extract.
6. The plant composition of claim 1, wherein the pansy water is prepared by: washing whole plant of herba Violae Hortensis, crushing, and squeezing.
7. Use of the plant composition according to any one of claims 1 to 6 in leave-on cosmetics.
CN202211372715.0A 2022-11-03 2022-11-03 Plant composition and preparation method thereof Active CN115554207B (en)

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