CN115537206A - Saline-alkali soil remediation improver and preparation method thereof - Google Patents
Saline-alkali soil remediation improver and preparation method thereof Download PDFInfo
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- CN115537206A CN115537206A CN202210999065.6A CN202210999065A CN115537206A CN 115537206 A CN115537206 A CN 115537206A CN 202210999065 A CN202210999065 A CN 202210999065A CN 115537206 A CN115537206 A CN 115537206A
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- soil remediation
- saline
- preparation
- alkali soil
- remediation improver
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- 239000002689 soil Substances 0.000 title claims abstract description 57
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- 239000003513 alkali Substances 0.000 title claims abstract description 34
- 238000005067 remediation Methods 0.000 title claims abstract description 34
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 30
- 102000004190 Enzymes Human genes 0.000 claims abstract description 22
- 108090000790 Enzymes Proteins 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000005507 spraying Methods 0.000 claims abstract description 17
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 16
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000002509 fulvic acid Substances 0.000 claims abstract description 16
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 16
- 239000000126 substance Substances 0.000 claims abstract description 11
- 230000002262 irrigation Effects 0.000 claims abstract description 3
- 238000003973 irrigation Methods 0.000 claims abstract description 3
- 229940088598 enzyme Drugs 0.000 claims description 21
- 238000000855 fermentation Methods 0.000 claims description 21
- 230000004151 fermentation Effects 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 20
- 241000235033 Zygosaccharomyces rouxii Species 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 8
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 claims description 6
- 102000013563 Acid Phosphatase Human genes 0.000 claims description 6
- 108010051457 Acid Phosphatase Proteins 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 239000004365 Protease Substances 0.000 claims description 6
- 229940106157 cellulase Drugs 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 108010046334 Urease Proteins 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000012136 culture method Methods 0.000 claims description 2
- 230000000052 comparative effect Effects 0.000 description 8
- 239000003337 fertilizer Substances 0.000 description 6
- 240000002853 Nelumbo nucifera Species 0.000 description 3
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 3
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 3
- 244000098338 Triticum aestivum Species 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 239000003674 animal food additive Substances 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 235000017643 Elaeagnus angustifolia Nutrition 0.000 description 1
- 244000016119 Elaeagnus latifolia Species 0.000 description 1
- 235000001456 Elaeagnus latifolia Nutrition 0.000 description 1
- 235000007630 Elaeagnus umbellata var parvifolia Nutrition 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241001278112 Populus euphratica Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Abstract
A saline-alkali soil remediation improver and a preparation method thereof belong to the technical field of soil remediation improvement, and comprise the following substances in parts by weight: 30 parts of yeast, 55 parts of fulvic acid and 15 parts of enzyme preparation. The application method of the soil remediation improver is that the soil remediation improver is sprayed on the ground before land preparation, watering or heavy rain fall, or is applied to the soil through drip irrigation, and the spraying amount is 3-6 kg/mu.
Description
Technical Field
The invention belongs to the field of soil remediation and improvement, and particularly relates to a saline-alkali soil remediation improver and a preparation method thereof.
Background
The saline-alkali soil is a land containing more salt, and due to excessive development of people on the land, the area of the saline-alkali soil is continuously enlarged, so that the saline-alkali soil is widely distributed in the world at present, and a large amount of saline, dry and hardened saline-alkali soil exists everywhere in all areas from cold zones, warm zones to tropical zones. As a big agricultural country, china needs to apply more chemical fertilizers to improve the crop yield to meet the food demand of the nation because of more people and less land and insufficient soil nutrient supply, but at the same time, the blindly use of the chemical fertilizers aggravates the salinization degree of the soil, so that the area of the saline-alkali soil in China reaches 9913 hectare, and the soil restoration and improvement of the saline-alkali soil are in urgent need of solution.
In recent years, a large amount of funds are invested by governments, and various methods are adopted to improve saline-alkali soil, and commonly used methods include a chemical improvement method and planting saline-alkali tolerant vegetation such as narrow-leaved oleaster, populus diversifolia and the like in the saline-alkali soil, wherein the chemical improvement method has good effect but higher cost; the planting of saline-alkali tolerant vegetation is environment-friendly, but has slow effect. So that the soil remediation and improvement still has no specific solution.
Disclosure of Invention
The invention provides a saline-alkali soil remediation improver and a preparation method thereof, which are used for overcoming the defects in the prior art.
The invention is realized by the following technical scheme:
the saline-alkali soil remediation improver and the preparation method thereof comprise the following substances in parts by weight: 30 parts of yeast, 55 parts of fulvic acid and 15 parts of enzyme preparation.
The yeast is zygosaccharomyces rouxii.
The enzyme preparation comprises any one or a mixture of any two or more of cellulase, protease, acid phosphatase and urease in any proportion.
The culture method of the yeast comprises the following steps:
the method comprises the following steps: preparing YPD culture medium;
step two: inoculating 1% of activated zygosaccharomyces rouxii bacterial liquid to a YPD culture medium, controlling the fermentation temperature to be 27-29 ℃ and the culture time to be 3-5 days;
step three: when the viable count of the zygosaccharomyces rouxii in the culture medium exceeds 8 multiplied by 10 5 And (5) stopping culturing when the concentration is CFU/g, and taking out the yeast liquid for later use.
The preparation method of the soil remediation improver comprises the following steps:
the method comprises the following steps: accurately weighing fulvic acid, an enzyme preparation and a yeast liquid;
step two: mixing the fulvic acid weighed in the first step, an enzyme preparation and a yeast liquid in a fermentation tank, stirring at the speed of 120r/min for 0.5h, and standing for fermentation for 12h;
step three: and (5) repeating the stirring and standing fermentation operations in the step two for 2 times to obtain the product.
The application method of the soil remediation improver comprises the steps of spraying the soil on the ground before land preparation, or before watering or before raining, or applying the soil by drip irrigation, wherein the spraying amount is 3-6 kg/mu.
The invention has the advantages that: the invention provides a saline-alkali soil remediation improver and a preparation method thereof.
The cellulase in the enzyme preparation can accelerate the decomposition of fibers such as hemp, straws, sugarcanes, bean dregs, vegetable and fruit residues, rotten plant roots and the like in the saline-alkali soil; proteases are involved in the transformation of amino acids, proteins and other protein-containing organic compounds present in the soil, the hydrolysis products of which are one of the nitrogen sources of higher plants; the acid phosphatase can decompose organic phosphorus in soil and promote decomposition and cyclic utilization of the organic phosphorus in crop tissues; the urease can improve the utilization rate of urea nitrogen in the soil.
The zygosaccharomyces rouxii has the characteristic of high osmotic pressure resistance, can improve the soil structure in a fermentation mode, loosen the soil and improve the utilization rate of the fertilizer.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1: a saline-alkali soil remediation improver and a preparation method thereof are characterized in that: the feed additive comprises the following substances in parts by weight: 30 parts of yeast, 55 parts of fulvic acid and 15 parts of enzyme preparation.
Specifically, the yeast described in this embodiment is zygosaccharomyces rouxii.
The enzyme preparation described in this embodiment comprises a mixture of cellulase, protease, acid phosphatase, and urease in a mass ratio of 2.
The method for culturing the yeast comprises the following steps:
the method comprises the following steps: preparing YPD culture medium;
step two: inoculating 1% of activated zygosaccharomyces rouxii bacterial liquid to a YPD culture medium, controlling the fermentation temperature to be 27 ℃ and the culture time to be 5 days;
step three: when the viable count of the zygosaccharomyces rouxii in the culture medium reaches 8 multiplied by 10 5 And (5) stopping culturing when the concentration is CFU/g, and taking out the yeast liquid for later use.
The preparation method of the soil remediation improver comprises the following steps:
the method comprises the following steps: accurately weighing fulvic acid, an enzyme preparation and a yeast liquid;
step two: mixing the fulvic acid weighed in the step one, an enzyme preparation and a yeast bacterial liquid in a fermentation tank, stirring at the speed of 120r/min for 0.5h, and standing for fermentation for 12h;
step three: and repeating the stirring and standing fermentation operations in the second step for 2 times to obtain the product.
Before land preparation, the product is sprayed on the ground, the spraying amount is 3 kg/mu, the spraying place is a development area Ma Zhuangcun in Juye county in lotus city of Shandong province, the spraying area is 2.5 mu, and crops are planted: a watermelon is provided.
Comparative example 1: the difference from example 1 is that comparative example 1 applied the same amount of commercial fertilizer, the spraying place was Ma Zhuangcun, a development area of Juye county, netherlands, shandong province, and the spraying area was 2.5 mu.
Example 2: a saline-alkali soil remediation improver and a preparation method thereof are characterized in that: the feed additive comprises the following substances in parts by weight: 30 parts of saccharomycetes, 55 parts of fulvic acid and 15 parts of enzyme preparation.
Specifically, the yeast described in this embodiment is zygosaccharomyces rouxii.
The enzyme preparation described in this embodiment comprises a mixture of cellulase, protease, acid phosphatase, and urease in a mass ratio of 1.
The method for culturing yeast described in this embodiment includes the following steps:
the method comprises the following steps: preparing YPD culture medium;
step two: inoculating 1% of activated zygosaccharomyces rouxii liquid to a YPD culture medium, controlling the fermentation temperature to be 28 ℃ and the culture time to be 4 days;
step three: when the viable count of the zygosaccharomyces rouxii in the culture medium reaches 8 multiplied by 10 5 And (5) stopping culturing when the concentration is CFU/g, and taking out the yeast liquid for later use.
The preparation method of the soil remediation improver comprises the following steps:
the method comprises the following steps: accurately weighing fulvic acid, an enzyme preparation and a yeast liquid;
step two: mixing the fulvic acid weighed in the first step, an enzyme preparation and a yeast liquid in a fermentation tank, stirring at the speed of 120r/min for 0.5h, and standing for fermentation for 12h;
step three: and repeating the stirring and standing fermentation operations in the second step for 2 times to obtain the product.
The product is sprayed on the ground before watering, the spraying dosage is 6 kg/mu, the spraying site is Zhang Cun west developing area of huge county of lotus city, shandong province, the spraying area is 2.5 mu, and crops are planted: wheat (Triticum aestivum L.).
Comparative example 2: the difference from example 2 is that comparative example 2 applied the same amount of commercial fertilizer, the spraying place was Ma Zhuangcun, a development area of Juye county, netherlands, shandong province, and the spraying area was 2.5 mu.
Example 3: a saline-alkali soil remediation improver and a preparation method thereof are characterized in that: the feed additive comprises the following substances in parts by weight: 30 parts of yeast, 55 parts of fulvic acid and 15 parts of enzyme preparation.
Specifically, the yeast described in this embodiment is zygosaccharomyces rouxii.
The enzyme preparation described in this embodiment comprises a mixture of cellulase, protease, and acid phosphatase in a mass ratio of 2.
The method for culturing the yeast comprises the following steps:
the method comprises the following steps: preparing a YPD culture medium;
step two: inoculating 1% of activated zygosaccharomyces rouxii liquid to a YPD culture medium, controlling the fermentation temperature to be 28 ℃ and the culture time to be 4 days;
step three: when the viable count of the zygosaccharomyces rouxii in the culture medium reaches 9 multiplied by 10 5 And (5) stopping culturing when the concentration is CFU/g, and taking out the yeast liquid for later use.
The preparation method of the soil remediation improver comprises the following steps:
the method comprises the following steps: accurately weighing fulvic acid, an enzyme preparation and a yeast liquid;
step two: mixing the fulvic acid weighed in the first step, an enzyme preparation and a yeast liquid in a fermentation tank, stirring at the speed of 120r/min for 0.5h, and standing for fermentation for 12h;
step three: and repeating the stirring and standing fermentation operations in the second step for 2 times to obtain the product.
Before watering, the product is sprayed on the ground, the spraying amount is 6 kg/mu, the spraying place is Maloucun in the development area of Juye county in lotus city, shandong province, the spraying area is 2.5 mu, and crops are planted: corn.
Comparative example 3: the difference from example 3 is that in comparative example 3, a commercial chemical fertilizer was applied in the same amount, and the spray area was 2.5 mu in Malou village, a development district of Juyowa, neze, shandong province.
TABLE 1 mu yield of different crops in moderate saline-alkali soil
As can be seen from Table 1, the yield per mu of watermelon in example 1 is 277kg higher than that in comparative example 1; the wheat yield per mu in example 2 was increased by about 30% compared to comparative example 2; the corn acre yield in the embodiment 3 is increased by 40 percent compared with the corn acre yield in the comparison 3, which shows that the saline-alkali soil remediation improver disclosed by the application can effectively solve the problem of saline-alkali soil hardening and improve the crop yield.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (6)
1. A saline-alkali soil remediation improver and a preparation method thereof are characterized in that: the composition comprises the following substances in parts by weight: 30 parts of yeast, 55 parts of fulvic acid and 15 parts of enzyme preparation.
2. The saline-alkali soil remediation improver and the preparation method thereof according to claim 1, characterized in that: the yeast is zygosaccharomyces rouxii.
3. The saline-alkali soil remediation improver and the preparation method thereof as claimed in claim 1, wherein the saline-alkali soil remediation improver comprises: the enzyme preparation comprises any one or a mixture of any two or more of cellulase, protease, acid phosphatase and urease which are mixed in any proportion.
4. The saline-alkali soil remediation improver and the preparation method thereof as claimed in claim 1, wherein the saline-alkali soil remediation improver comprises: the culture method of the yeast comprises the following steps:
the method comprises the following steps: preparing YPD culture medium;
step two: inoculating 1% of activated zygosaccharomyces rouxii bacterial liquid to a YPD culture medium, controlling the fermentation temperature to be 27-29 ℃ and the culture time to be 3-5 days;
step three: when the viable count of the zygosaccharomyces rouxii in the culture medium exceeds 8 multiplied by 10 5 And (5) stopping culturing when the concentration is CFU/g, and taking out the yeast liquid for later use.
5. The saline-alkali soil remediation improver and the preparation method thereof as claimed in claim 1, wherein the saline-alkali soil remediation improver comprises: the preparation method of the soil remediation improver comprises the following steps:
the method comprises the following steps: accurately weighing fulvic acid, an enzyme preparation and a yeast liquid;
step two: mixing the fulvic acid weighed in the step one, an enzyme preparation and a yeast bacterial liquid in a fermentation tank, stirring at the speed of 120r/min for 0.5h, and standing for fermentation for 12h;
step three: and repeating the stirring and standing fermentation operations in the second step for 2 times to obtain the product.
6. The saline-alkali soil remediation improver and the preparation method thereof as claimed in claim 1, wherein the saline-alkali soil remediation improver comprises: the application method of the soil remediation improver is that the soil remediation improver is sprayed on the ground before land preparation, watering or heavy rain fall, or is applied to the soil through drip irrigation, and the spraying amount is 3-6 kg/mu.
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CN202210999065.6A CN115537206A (en) | 2022-08-19 | 2022-08-19 | Saline-alkali soil remediation improver and preparation method thereof |
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CN111675573A (en) * | 2020-06-24 | 2020-09-18 | 华仲动芯新材料(盱眙)有限公司 | Tea garden alkaline soil improvement agent and preparation method thereof |
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JP2003335792A (en) * | 2002-05-20 | 2003-11-28 | Tokiwa Zoen Kk | Humus precursor-ferrihydrite precursor complex, method for producing the same, method for treating livestock waste using humus precursor-ferrihydrite precursor complex and sewage disposal system |
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