CN109504395A - A kind of alkaline land soil biological modification agent and its preparation method and application - Google Patents
A kind of alkaline land soil biological modification agent and its preparation method and application Download PDFInfo
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Abstract
The invention discloses a kind of alkaline land soil biological modification agents and its preparation method and application, wherein modifying agent is made of modifying agent component A and modifying agent component B, modifying agent component A according to percent by volume include: 2%~8% cellulase, 3%~8% lignoenzyme, 2%~8% protease, 5%~10% trichoderma viride bacteria suspension, 5%~10% Candida tropicalis bacteria suspension, 8%~15% bacillus megaterium bacteria suspension, 8%~12% colloid bacillus cereus bacteria suspension, surplus be biochemical fulvic acid liquid；Modifying agent component B includes 12%~15% water-soluble lubricating liquid, 7%~9% water-soluble metal chelating agent, 2%~4% hydrogel synergist, 3%~5% carbon dioxide foaming agent, 0.5%~1.5% dispersing agent, 10%~12% biomass extract according to percent by volume, and surplus is temperature-sensitive hydrogel particle.The present invention compounding application, soil alkaline can be improved situation before planting and when plantation using microorganism and temperature-sensitive hydrogel, and make plant yield-increasing.
Description
Technical field
The invention belongs to soil remediation and improving technology fields, and in particular to a kind of alkaline land soil biological modification agent and its
Preparation method and application.
Background technique
The salinization of soil and Secondary Saline problem are worldwide widely present, especially arid, semiarid zone,
Problem is even more serious.According to UNESCO (UNESCO) and food and agricultural organization (FAO) incomplete statistics, whole world salt-soda soil
Area is 9.54 hundred million hectares, wherein China is 99,130,000 hectares.Chinese salt-soda soil is large in number and widely distributed, northwest, North China, West of The Northeast China and
Coastal region is all distributed, the saline Land edaphic condition that not only damage to crops depends on for existence, but also inhibits the growth of crop
Development causes to be short of seedling, the underproduction, death, to hinder the development of agricultural production.The serious area of saline Land, causes big face
Product farmland is no longer cultivated, uncultivated, wastes a large amount of land resource.The salinization of soil and Secondary Saline problem, have become the world
The resource constraints of Irrigation farming sustainable development.
Salt-soda soil briefly, can be divided into light salt-soda soil, moderate saline-alkali soil and heavy saline using in process.Gently
Salt-soda soil refers to its emergence rate 70%~80%, its salt content is below 3/1000ths；Heavy saline refers to its saliferous
For amount more than 6/1000ths, emergence rate is lower than 50%；This intermediate block is exactly that moderate saline-alkali soil (is indicated are as follows: slight alkaline land with pH value
PH value are as follows: 7.1~8.5, moderate saline-alkali soil pH value are as follows: 8.5~9.5, heavy salinized ground pH value are as follows: 9.5 or more).
The measure for administering salt-soda soil at present mainly has water conservancy ameliorative measure (irrigate, drain, warping, planting rice, antiseepage etc.), agriculture
Industry ameliorative measure (level land, improve farming, apply soil moved in improve the original, fertilising, sowing, crop rotation, the interplanting of kind etc.), biological modification measure
(plantation salt-tolerant plant and herbage, green manure, afforestation etc.) and chemical modifying measure (application upgrade materials, such as gypsum, phosphorus stone
Cream, calcium sulfite etc.) etc. four aspect.Since each measure has the certain scope of application and condition, and cannot solve at all
The certainly sprawling and development of the salinization of soil.Therefore, above-mentioned measure has great limitation.
Summary of the invention
The technical solution of the present invention is as follows: a kind of alkaline land soil biological modification agent, by modifying agent component A and modifying agent component
B composition,
The modifying agent component A according to percent by volume include: 2%~8% cellulase, 3%~8% lignoenzyme,
2%~8% protease, 5%~10% trichoderma viride bacteria suspension, 5%~10% Candida tropicalis bacteria suspension, 8%~
15% bacillus megaterium bacteria suspension, 8%~12% colloid bacillus cereus bacteria suspension, surplus are biochemical fulvic acid liquid, wherein green
Viable count >=108cfu/mL in color Trichoderma bacteria suspension, viable count >=108cfu/mL in Candida tropicalis bacteria suspension, it is huge
Viable count >=1010cfu/mL in Bacterium anthracoides bacteria suspension, viable count >=1010cfu/mL in colloid bacillus cereus bacteria suspension；
The modifying agent component B according to percent by volume includes 12%~15% water-soluble lubricating liquid, 7%~9% water-soluble
Property metal-chelator, 2%~4% hydrogel synergist, 3%~5% carbon dioxide foaming agent, 0.5%~1.5% dispersing agent,
10%~12% biomass extract, surplus are temperature-sensitive hydrogel particle.
Further, the modifying agent component A's the preparation method comprises the following steps:
A1: trichoderma viride, Candida tropicalis, bacillus megaterium, colloid bacillus cereus are expanded respectively
Culture, respectively obtains four kinds of culture solutions；
The culture solution: being inoculated with by 10%~15% volume carry out Liquid Culture respectively into fermentor again by A2, training
25~35 DEG C, 24~48h of incubation time of temperature is supported, obtains four kinds of bacteria suspensions respectively；
A3: by four kinds of bacteria suspensions, cellulase solution, lignin enzyme solution, liquid of protease, biochemical fulvic acid liquid according to modifying agent
The ratio of component A is mixed.Wherein, cellulase, lignoenzyme, protease, trichoderma viride bacteria suspension, the torrid zone are false
Silk saccharomycete bacteria suspension, bacillus megaterium bacteria suspension, colloid bacillus cereus bacteria suspension, biochemical fulvic acid liquid are commercially available production
Product.Modifying agent component A of the invention carries out alkaline land soil biological modification using the complex microorganism of bacterium and fungi, on the one hand
Using trichoderma viride, candida tropicalis, bacillus megaterium, colloid bacillus cereus and its metabolite activating soil, promote
Into the exchange of salt ion, is conducive to salt ion and is absorbed by plants or is moved down with moisture to deep subsoil, fulvic acid is conducive to soil
The reduction of pH value；On the other hand there is activating soil nutrition using beneficial microbe and biochemical fulvic acid, promote crop root raw
It is long, be conducive to plant growth.
Further, the modifying agent B component the preparation method comprises the following steps:
B1: the temperature-sensitive hydrogel and hydrogel synergist are mixed according to the above mentioned proportion uniformly, protected in inert atmosphere
Under shield, 100~130 DEG C of water vapour 20~30min of high-temperature process are passed through, are then cooled to 10~20 DEG C with 10 DEG C/min, and protect
10~20min of temperature, repeats the above steps 3~5 times, obtains synergy temperature-sensitive hydrogel；By hot and cold alternation, so that temperature sensitive type water
Gel is alternately expanded and shrinkage, is conducive to sufficiently act on hydrogel synergist, improves its surface-active and is swollen back
Elastic energy, and increase its load capacity.
B2: dispersing agent is added in the water-soluble metal chelating agent and is uniformly mixed, 100~200 times of volumes are added
The purified water of ratio is diluted, and as dialyzate, the synergy temperature-sensitive hydrogel is fitted into bag filter, the dialysis is utilized
Liquid is dialysed, and is stirred continuously in dialysis procedure, the time be 12~for 24 hours, temperature be 4~10 DEG C；Made using dispersing agent water-soluble
Property metal-chelator keeps homogeneously dispersed state in dilution, meanwhile, make water-soluble metal chelating agent abundant using dialysis
It is supported in temperature-sensitive hydrogel.
B3: it by the synergy temperature-sensitive hydrogel after dialysis under vacuum and 35~40 DEG C of environmental conditions, is purged using nitrogen
15~20min can not only remove the excessive moisture of dialyzate, due also to being higher than the transformation of temperature-sensitive hydrogel at 35~40 DEG C
At a temperature of, volume can be shunk, and combined and be greatly improved water-soluble metal chelating agent and synergy temperature under vacuum pressure condition
The intensity of load of quick type hydrogel, prevents it from falling off；
B4: adding the biomass extract, water-soluble lubricating liquid stirs evenly, and is eventually adding the carbon dioxide hair
Infusion is to get modifying agent component B.
Further, it according to volume ratio is 1:2 that the hydrogel synergist, which is biological active organic silicon and microbial oil,
It mixes.
Further, the biomass extract is by marine algae extract, dregs of fat extract, soybean meal extractive, Ipomoea batatas Lam
Extract, chicory extract are that 1:3:2:1:2 mixing is formed through Pintsch process again according to mass ratio.Biomass extract can be soil
Earth provides nutriment, improves soil fertility.
Further, the technological parameter of the Pintsch process are as follows: cracking initial temperature is 300 DEG C, and gradient increased temperature is extremely
500 DEG C, the time is 10~20min, and the biomass after high temperature understands can further be concentrated, and is more favorable for plant absorption.Period
Aeration stirring is carried out by high-pressure water vapor, biomass carbonization can be prevented.
Further, the water-soluble lubricating liquid is hyalomitome acid type lubricating fluid, chitosan type lubricating fluid, aloe type lubrication
Any one or a few in liquid combines.The penetration of modifying agent component B can be improved in water-soluble lubricating liquid.
The present invention also provides a kind of methods for carrying out alkaline land soil improvement using above-mentioned modifying agent, including following step
It is rapid:
(1) before planting, salt-soda soil is deep ploughed and 40~50cm and is digged, according to the dosage of 20L per acre, by modifying agent
Component B is added 40~45 DEG C of water and dilutes 30~50 times, and carries out bubble processing of vibrating using ultrasonic wave bubbler, by modifying agent group
The foam of part B replace with soil is laid on dell bottom, and depth of foam is 8~10cm when laying, and thickness of soil is 5~10cm；
According to the dosage of 100~200ml per jin, modifying agent component A is diluted with water 200~500 times, is carried out after impregnating 4~8h of seed
Sowing, and the dilution after seed is impregnated is sprayed at soil surface；Foam is finer and smoother compared to water droplet, and penetration is strong and equal
Even, under the conditions of 40~45 DEG C, temperature-sensitive hydrogel is conducive to permeate in soil crack after shrinking, and after covering soil, with
The decline of temperature, temperature-sensitive hydrogel volume starts to expand, convenient for being located in soil crack, without accumulating, be convenient for its
The metal-chelator of load carries out chelation treatment to the salt ion in surrounding soil, also for the microorganism in modifying agent component A
Microbial inoculum provides the matrix quickly bred, moreover it is possible to have certain water-holding capacity.
(2) in plantation, the irrigation canals and ditches that one of depth is 10~12cm are being dug at 15~20cm of plant root, according to every
Modifying agent component B is added 40~45 DEG C of water and dilutes 30~50 times, and shaken using ultrasonic wave bubbler by the dosage of mu 10L
Bubble processing is smash, foam is laid in irrigation canals and ditches, and adds soil covering；According still further to the dosage of 5~10L per acre, by modifying agent component A
100~300 times are diluted with water, is poured on irrigation canals and ditches earthing.
It further, can also be by modifying agent component A according to fermenting 30 in 40~80 liters/1000kg farmyard manure in plantation
It or so carries out 800kg~1500kg per acre and applies fertilizer.Microorganism fungus kind can be supplemented again by fertilising, improve salt-soda soil
Improved effect.
The biology that alkaline land soil biological modification agent of the invention is applicable in salt-soda soil, salinisation and basic soil etc. changes
It is good.
Compared with prior art, the invention has the benefit that
(1) present invention carries out compounding application before planting and when plantation using microorganism and temperature-sensitive hydrogel, can
With substantially improving soil alkaline the case where, and improve the yield of plant.
(2) present invention using hydrogel synergist to it by under hot and cold alternation environment, carrying out temperature-sensitive hydrogel
Activation modification processing improves its surface-active and swelling resilience performance, and increases the capacity of its carried metal chelating agent, moreover it is possible to be
The microorganism applied afterwards provides breeding base, it is promoted quickly to breed, and also has certain water-holding capacity, in addition, temperature sensitive type water-setting
Glue is biodegradable, Nonpoisonous, non-environmental-pollution.
(3) microorganism fungus kind selected by the present invention has stronger soil and adaptive capacity to environment, and after application, bacterial strain can
It colonizes in the soil rapidly, is effectively improved edaphon Microflora；And have between selected microorganism fungus kind good
Collaboration growth-promoting functions, can guarantee the viable count and stability of complex micro organism fungicide, improve soil activating ability；
(4) enzyme preparation selected by the present invention is conducive to the decomposition of the soil organism, provides for the growth of edaphon
Nutriment promotes the improvement of ecological environment of soil；
(5) biochemical fulvic acid (BFA) selected by the present invention has the characteristics that molecular weight is small, active group is high, can simulate
Nature humic acid formed environment, multiple-microorganism is inoculated into crop growth environment, have improvement soil, improve ground temperature,
The effect of promote well developed root system, effectively adjust soil acidity or alkalinity, solve soil hardening etc.；In dry situation, have
Reduce the effect of crop Stomatal complexes；
(6) alkaline land soil biological modification agent of the invention can substitute chemical agent completely, and when use is pollution-free, without residual
It stays, without eco-toxicity, reduces salt-soda soil pH value, reduce salt ionic concentration, improve 30% or more plant emergence rate, make crop yield
15% or more.
Specific embodiment
Technical solution of the present invention and using effect are described further below in conjunction with specific example.Employed in example
Strain is purchased from Chinese agriculture Microbiological Culture Collection administrative center (ACCC) Chinese industrial Culture Collection respectively
(CICC) and Guangdong Microbiological Culture Collection administrative center (GIM), bacterium numbering are as follows: trichoderma viride (ACCC-30169), heat
Band Candida (CICC-1798), bacillus megaterium (ACCC-01040), colloid bacillus cereus (GIM-16).Each strain is outstanding
Routinely preparation method obtains liquid, makes viable count >=10 in trichoderma viride bacteria suspension8Cfu/mL, Candida tropicalis bacterium are outstanding
Viable count >=10 in liquid8Cfu/mL, viable count >=10 in bacillus megaterium bacteria suspension10Cfu/mL, colloid bacillus cereus bacterium are outstanding
Viable count >=10 in liquid10cfu/mL.Cellulase solution, lignin enzyme solution, liquid of protease market are commercially available, biochemical fulvic acid liquid
It is produced for Zhengzhou City's profit up to Chemical Co., Ltd..
Embodiment 1
It prepares modifying agent component A: taking 5% trichoderma viride, 5% Candida tropicalis, 8%~15% huge by volume
Bacterium anthracoides, 8% colloid bacillus cereus expand culture respectively, respectively obtain four kinds of culture solutions；Culture solution is pressed again
10% volume is inoculated with carries out Liquid Culture into fermentor respectively, and 25 DEG C of cultivation temperature, incubation time for 24 hours, obtains four respectively
Kind bacteria suspension；By four kinds of bacteria suspensions: 2% cellulase solution, 3% lignin enzyme solution, 2% liquid of protease, 67% biochemical fulvic acid
Liquid is mixed according to the ratio of modifying agent component A.
It prepares modifying agent component B:65.5% temperature-sensitive hydrogel to be uniformly mixed with 2% hydrogel synergist, in indifferent gas
Under atmosphere protection, 100 DEG C of water vapour high-temperature process 20min are passed through, are then cooled to 10 DEG C with 10 DEG C/min, and keep the temperature 10min, weight
Multiple above-mentioned steps 3 times, obtain synergy temperature-sensitive hydrogel；By hot and cold alternation, so that temperature-sensitive hydrogel progress is alternately swollen
Swollen and shrinkage is conducive to sufficiently act on hydrogel synergist, improves its surface-active and swelling resilience performance, and it is negative to increase it
Carry capacity.Wherein, it according to volume ratio is that 1:2 is mixed that hydrogel synergist, which is biological active organic silicon and microbial oil,.
0.5% dispersing agent is added in 7% water-soluble metal chelating agent to be uniformly mixed, 100 times of volume ratios are added
Purified water is diluted, and as dialyzate, synergy temperature-sensitive hydrogel is fitted into bag filter, is dialysed using dialyzate,
It is stirred continuously in dialysis procedure, time 12h, temperature is 4 DEG C；Make water-soluble metal chelating agent in dilution using dispersing agent
Homogeneously dispersed state is kept, meanwhile, using dialysis water-soluble metal chelating agent is sufficiently supported in temperature-sensitive hydrogel.
By the synergy temperature-sensitive hydrogel after dialysis under vacuum and 35 DEG C of environmental conditions, 15min is purged using nitrogen, no
The excessive moisture of dialyzate can be only removed, due also in the case where 35 DEG C are higher than the transition temperatures of temperature-sensitive hydrogel, volume can be into
Row is shunk, and combines the load that water-soluble metal chelating agent Yu synergy temperature-sensitive hydrogel are greatly improved under vacuum pressure condition
Intensity prevents it from falling off；
Add 10% biomass extract, 12% water-soluble lubricating liquid stirs evenly, be eventually adding 3% carbon dioxide hair
Infusion is to get modifying agent component B.Wherein, biomass extract is by marine algae extract, dregs of fat extract, soybean meal extractive, red
Potato root extract, chicory extract are mixed according to mass ratio for 1:3:2:1:2.Water-soluble lubricating liquid is hyalomitome acid type profit
Synovia.The penetration of modifying agent component B can be improved in water-soluble lubricating liquid.
Using watermelon as experimental subjects:
(1) before planting, salt-soda soil is deep ploughed and 40cm and is digged, according to the dosage of 20L per acre, by modifying agent component
B is added 40 DEG C of water and dilutes 30 times, and is handled using the ultrasonic wave bubbler bubble that vibrate, by the foam of modifying agent component B with
Soil is alternately laid on dell bottom, and depth of foam is 8cm, thickness of soil 5cm when laying；According to the dosage of 100ml per jin,
Modifying agent component A is diluted with water 200 times, is sowed after impregnating seed 4h, and the dilution after seed is impregnated is sprayed at
Soil surface；Foam is finer and smoother compared to water droplet, and penetration is strong and uniform, and under the conditions of 40 DEG C, temperature-sensitive hydrogel is shunk
It is conducive to permeate in soil crack afterwards, and after covering soil, with the decline of temperature, temperature-sensitive hydrogel volume starts to expand
Greatly, convenient for being located in soil crack, without accumulating, convenient for its load metal-chelator to the salt ion in surrounding soil
Chelation treatment is carried out, the matrix quickly bred is provided also for the microbial bacterial agent in modifying agent component A, moreover it is possible to have certain storage
Outlet capacity.
(2) in plantation, the irrigation canals and ditches that one of depth is 10cm are being dug at plant root 15cm, according to 10L's per acre
Modifying agent component B is added 40 DEG C of water and dilutes 30 times, and carries out bubble processing of vibrating using ultrasonic wave bubbler by dosage, will be steeped
Foam is laid in irrigation canals and ditches, and adds soil covering；According still further to the dosage of 5L per acre, modifying agent component A is diluted with water 100 times, is poured
In on irrigation canals and ditches earthing.
Effect: using the watermelon emergence rate of experimental group of the present invention as 85%, and the emergence rate for not improveing control group is 45%；It is real
Test increasing yield of water melons 50% of the group than control group.
Embodiment 2
The present embodiment is substantially the same manner as Example 1, the difference is that:
(1) modifying agent component A according to percent by volume include: 5% cellulase, 5.5% lignoenzyme, 5% protease,
7.5% trichoderma viride bacteria suspension, 7.5% Candida tropicalis bacteria suspension, 11.5% bacillus megaterium bacteria suspension, 10%
Colloid bacillus cereus bacteria suspension, 48% biochemical fulvic acid liquid；
(2) modifying agent component B includes 13.5% water-soluble lubricating liquid, 8% water-soluble metal chelating according to percent by volume
Agent, 3% hydrogel synergist, 4% carbon dioxide foaming agent, 1% dispersing agent, 11% biomass extract, 59.5% temperature sensitive type
Hydrogel fines.
(3) water-soluble lubricating liquid is chitosan type lubricating fluid.
(4) it using corn as subjects, before planting, by salt-soda soil deep ploughing 45cm and digs, according to 20L's per acre
Modifying agent component B is added 43 DEG C of water and dilutes 40 times, and carries out bubble processing of vibrating using ultrasonic wave bubbler by dosage, will be changed
The foam of good dose of component B replaces with soil is laid on dell bottom, and depth of foam is 9cm, thickness of soil 8cm when laying；It presses
According to the dosage of 150ml per jin, modifying agent component A is diluted with water 350 times, is sowed after impregnating seed 6h, and seed is soaked
Dilution after bubble is sprayed at soil surface；Foam is finer and smoother compared to water droplet, and penetration is strong and uniform, in 43 DEG C of conditions
Under, temperature-sensitive hydrogel is conducive to permeate in soil crack after shrinking, and after covering soil, it is temperature sensitive with the decline of temperature
Type hydrogel volume starts to expand, convenient for being located in soil crack, without accumulating, convenient for the metal-chelator pair of its load
Salt ion in surrounding soil carries out chelation treatment, quickly breeds also for the microbial bacterial agent offer in modifying agent component A
Matrix, moreover it is possible to have certain water-holding capacity.
In plantation, the irrigation canals and ditches that one of depth is 11cm are being dug at plant root 18cm, according to the use of 10L per acre
Modifying agent component B is added 43 DEG C of water and dilutes 40 times, and carries out bubble processing of vibrating using ultrasonic wave bubbler by amount, by foam
It is laid in irrigation canals and ditches, and adds soil covering；According still further to the dosage of 8L per acre, modifying agent component A is diluted with water 200 times, pour in
On irrigation canals and ditches earthing.Experimental group is the method for the present embodiment, and control group is only to be carried out with the modifying agent component A of the present embodiment to soil
Processing, blank group are without any processing.
Effect: the pH value of experimental group is 7.56, Na++K+For 0.24g/kg, emergence rate 86.1%, yield 504kg/
Mu；
The pH value of control group is 7.89, Na++K+For 0.54g/kg, emergence rate 80.2%, yield is 423kg/ mus；
Blank group pH value is 8.45, Na++K+For 1.74g/kg, emergence rate 50.2%, yield is 282kg/ mus；
In contrast, experimental group than control group corn yield increasing 19.1%, than the corn yield increasing 78.7% of blank group.
Embodiment 3
The present embodiment is substantially the same manner as Example 1, the difference is that:
(1) modifying agent component A according to percent by volume include: 8% cellulase, 8% lignoenzyme, 8% protease,
10% trichoderma viride bacteria suspension, 10% Candida tropicalis bacteria suspension, 15% bacillus megaterium bacteria suspension, 12% colloid
Bacillus bacteria suspension, 29% biochemical fulvic acid liquid；
(2) modifying agent component B according to percent by volume include 15% water-soluble lubricating liquid, 9% water-soluble metal chelating agent,
4% hydrogel synergist, 5% carbon dioxide foaming agent, 1.5% dispersing agent, 10%~12% biomass extract, 535% temperature
Quick type hydrogel fines.
(3) water-soluble lubricating liquid is aloe type lubricating fluid.
(4) it using tomato as experimental subjects, before planting, by salt-soda soil deep ploughing 50cm and digs, according to 20L's per acre
Modifying agent component B is added 45 DEG C of water and dilutes 50 times, and carries out bubble processing of vibrating using ultrasonic wave bubbler by dosage, will be changed
The foam of good dose of component B replaces with soil is laid on dell bottom, and depth of foam is 10cm, thickness of soil 10cm when laying；
According to the dosage of 200ml per jin, modifying agent component A is diluted with water 500 times, is sowed after impregnating seed 8h, and by seed
Dilution after immersion is sprayed at soil surface；Foam is finer and smoother compared to water droplet, and penetration is strong and uniform, in 45 DEG C of conditions
Under, temperature-sensitive hydrogel is conducive to permeate in soil crack after shrinking, and after covering soil, it is temperature sensitive with the decline of temperature
Type hydrogel volume starts to expand, convenient for being located in soil crack, without accumulating, convenient for the metal-chelator pair of its load
Salt ion in surrounding soil carries out chelation treatment, quickly breeds also for the microbial bacterial agent offer in modifying agent component A
Matrix, moreover it is possible to have certain water-holding capacity.
In plantation, the irrigation canals and ditches that one of depth is 12cm are being dug at plant root 20cm, according to the use of 10L per acre
Modifying agent component B is added 45 DEG C of water and dilutes 50 times, and carries out bubble processing of vibrating using ultrasonic wave bubbler by amount, by foam
It is laid in irrigation canals and ditches, and adds soil covering；According still further to the dosage of 10L per acre, modifying agent component A is diluted with water 300 times, pour in
On irrigation canals and ditches earthing.
Effect: using the tomato emergence rate of experimental group of the present invention as 88%, and the emergence rate for not improveing control group is 54%；It is real
Test tomato production promoting 56% of the group than control group.
Embodiment 4
The present embodiment is substantially the same manner as Example 2, the difference is that:
Biomass extract in the present embodiment is mentioned by marine algae extract, dregs of fat extract, soybean meal extractive, Ipomoea batatas Lam
Object, chicory extract is taken to form again through Pintsch process according to mass ratio for 1:3:2:1:2 mixing.Biomass extract can be soil
Nutriment is provided, soil fertility is improved.The technological parameter of Pintsch process are as follows: cracking initial temperature is 300 DEG C, and gradient increased temperature is extremely
500 DEG C, time 15min, the biomass after high temperature understands can further be concentrated, and be more favorable for plant absorption.Period passes through
High-pressure water vapor carries out aeration stirring, can prevent biomass carbonization.
Effect: using the watermelon emergence rate of experimental group of the present invention as 91.5%, and the emergence rate for not improveing control group is 50%；
Increasing yield of water melons 71% of the experimental group than control group.
Embodiment 5
The present embodiment is substantially the same manner as Example 2, the difference is that:
(1) also in plantation, modifying agent component A's the present embodiment ferments 30 days or so according in 40 liters/1000kg farmyard manure,
800kg per acre is carried out to apply fertilizer.Microorganism fungus kind can be supplemented again by fertilising, improve the improved effect in salt-soda soil.Control group
Common farmyard manure processing is then only increased on the basis of embodiment 2.
Effect: being 7.21, Na through the present embodiment treated pH value++K+For 0.20g/kg, emergence rate 88.4%, yield
It is 545kg/ mus；Control group pH value is 7.56, Na++K+For 0.24g/kg, emergence rate 87.0%, yield is 516kg/ mus, phase
Than under, compare control group corn yield increasing 5.6% by the present embodiment.
Embodiment 6
The present embodiment is substantially the same manner as Example 2, the difference is that:
(1) also in plantation, modifying agent component A's the present embodiment ferments 30 days or so according in 80 liters/1000kg farmyard manure,
1500kg per acre is carried out to apply fertilizer.Microorganism fungus kind can be supplemented again by fertilising, improve the improved effect in salt-soda soil.Control group
Common farmyard manure processing is then only increased on the basis of embodiment 2.
Effect: being 7.06, Na through modifying agent of the present invention treated pH value++K+For 0.18g/kg, emergence rate 89.9%,
Yield is 567kg/ mus；Control group pH value is 7.56, Na++K+For 0.24g/kg, emergence rate 87.0%, yield 516kg/
Mu compares control group corn yield increasing 9.8% by the present embodiment in contrast.
The above, only presently preferred embodiments of the present invention, but scope of protection of the present invention is not limited thereto, it is any ripe
Those skilled in the art are known within the scope of the method that the present invention discloses, according to the method for the present invention and its inventive concept adds
With equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of alkaline land soil biological modification agent, which is characterized in that it is made of modifying agent component A and modifying agent component B,
The modifying agent component A according to percent by volume include: 2%~8% cellulase, 3%~8% lignoenzyme, 2%~
It is 8% protease, 5%~10% trichoderma viride bacteria suspension, 5%~10% Candida tropicalis bacteria suspension, 8%~15% huge
Bacterium anthracoides bacteria suspension, 8%~12% colloid bacillus cereus bacteria suspension, surplus are biochemical fulvic acid liquid, wherein Trichoderma viride
Viable count >=10 in bacterium bacteria suspension8Cfu/mL, viable count >=10 in Candida tropicalis bacteria suspension8Cfu/mL, huge gemma bar
Viable count >=10 in bacterium bacteria suspension10Cfu/mL, viable count >=10 in colloid bacillus cereus bacteria suspension10cfu/mL；
The modifying agent component B includes 12%~15% water-soluble lubricating liquid, 7%~9% water-soluble gold according to percent by volume
Belong to chelating agent, 2%~4% hydrogel synergist, 3%~5% carbon dioxide foaming agent, 0.5%~1.5% dispersing agent, 10%
~12% biomass extract, surplus are temperature-sensitive hydrogel particle.
2. a kind of alkaline land soil biological modification agent as described in claim 1, which is characterized in that the modifying agent component A's
The preparation method comprises the following steps:
A1: trichoderma viride, Candida tropicalis, bacillus megaterium, colloid bacillus cereus are expanded culture respectively,
Respectively obtain four kinds of culture solutions；
A2: the culture solution is inoculated with by 10%~15% volume carries out Liquid Culture, culture temperature respectively into fermentor again
25~35 DEG C, 24~48h of incubation time of degree obtains four kinds of bacteria suspensions respectively；
A3: by four kinds of bacteria suspensions, cellulase solution, lignin enzyme solution, liquid of protease, biochemical fulvic acid liquid according to modifying agent component
The ratio of A is mixed.
3. a kind of alkaline land soil biological modification agent as described in claim 1, which is characterized in that the modifying agent B component
The preparation method comprises the following steps:
B1: the temperature-sensitive hydrogel and hydrogel synergist are mixed according to the above mentioned proportion uniformly, under inert atmosphere protection,
100~130 DEG C of water vapour 20~30min of high-temperature process are passed through, are then cooled to 10~20 DEG C with 10 DEG C/min, and keep the temperature 10~
20min repeats the above steps 3~5 times, obtains synergy temperature-sensitive hydrogel；
B2: dispersing agent is added in the water-soluble metal chelating agent and is uniformly mixed, 100~200 times of volume ratios are added
Purified water is diluted, and as dialyzate, the synergy temperature-sensitive hydrogel is fitted into bag filter, using the dialyzate into
Row dialysis, is stirred continuously in dialysis procedure, and the time is 12~for 24 hours, temperature is 4~10 DEG C；
B3: by the synergy temperature-sensitive hydrogel after dialysis under vacuum and 35~40 DEG C of environmental conditions, using nitrogen purging 15~
20min, for increasing the intensity of load of water-soluble metal chelating agent Yu synergy temperature-sensitive hydrogel；
B4: adding the biomass extract, water-soluble lubricating liquid stirs evenly, and is eventually adding the blown with carbon dioxide
Agent is to get modifying agent component B.
4. a kind of alkaline land soil biological modification agent as claimed in claim 1 or 3, which is characterized in that the hydrogel synergy
It according to volume ratio is that 1:2 is mixed that agent, which is biological active organic silicon and microbial oil,.
5. a kind of alkaline land soil biological modification agent as claimed in claim 1 or 3, which is characterized in that the biomass is extracted
Liquid be by marine algae extract, dregs of fat extract, soybean meal extractive, sweet potato root extract, chicory extract according to mass ratio be 1:
3:2:1:2 mixing is formed through Pintsch process again.
6. a kind of alkaline land soil biological modification agent as claimed in claim 1 or 3, which is characterized in that the water-soluble lubricating
Liquid is hyalomitome acid type lubricating fluid, any one or a few in chitosan type lubricating fluid, aloe type lubricating fluid combines.
7. the method for carrying out alkaline land soil improvement using above-mentioned modifying agent, which comprises the following steps:
(1) before planting, salt-soda soil is deep ploughed and 40~50cm and is digged, according to the dosage of 20L per acre, by modifying agent component
B is added 40~45 DEG C of water and dilutes 30~50 times, and carries out bubble processing of vibrating using ultrasonic wave bubbler, by modifying agent component B
Foam replace with soil and be laid on dell bottom, depth of foam is 8~10cm when laying, and thickness of soil is 5~10cm；According to
Modifying agent component A is diluted with water 200~500 times, is sowed after impregnating 4~8h of seed by the dosage of 100~200ml per jin,
And the dilution after impregnating seed is sprayed at soil surface；
(2) in plantation, the irrigation canals and ditches that one of depth is 10~12cm are being dug at 15~20cm of plant root, according to per acre
Modifying agent component B is added 40~45 DEG C of water and dilutes 30~50 times, and vibrated using ultrasonic wave bubbler by the dosage of 10L
Bubble processing, foam is laid in irrigation canals and ditches, and adds soil covering；According still further to the dosage of 5~10L per acre, modifying agent component A is added
Water dilutes 100~300 times, pours on irrigation canals and ditches earthing.
8. the method for alkaline land soil improvement as claimed in claim 7, which is characterized in that, can also be by modifying agent in plantation
Component A ferments 30 days or so according in 40~80 liters/1000kg farmyard manure, carries out 800kg~1500kg per acre and applies fertilizer.
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