CN112898089A - Fish protein functional biological fertilizer and preparation method thereof - Google Patents
Fish protein functional biological fertilizer and preparation method thereof Download PDFInfo
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- CN112898089A CN112898089A CN202011627408.3A CN202011627408A CN112898089A CN 112898089 A CN112898089 A CN 112898089A CN 202011627408 A CN202011627408 A CN 202011627408A CN 112898089 A CN112898089 A CN 112898089A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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Abstract
The invention belongs to the technical field of biological fertilizer production, and particularly relates to a fish protein functional biological fertilizer and a preparation method thereof, wherein the fish protein functional biological fertilizer comprises the following components in parts by weight: 40-70 parts of fish paste, 8-20 parts of enzymolysis liquid, 1-5 parts of saccharomyces cerevisiae, 30-50 parts of urea, 10-30 parts of potassium fulvate, 30-50 parts of chitosan, 1-3 parts of ferrous sulfate, 1-3 parts of manganese sulfate, 1-3 parts of copper sulfate and 1-2 parts of molybdenum salt; the preparation method comprises the steps of raw material preparation, fermentation enzymolysis, centrifugal separation, concentration and drying. When the fish protein functional biological fertilizer prepared by the invention is used, the absorption balance of crops to various required nutrient elements can be promoted, various nutrient deficiency symptoms can be prevented, the leaves are dark green, the stems are thick and strong, the photosynthesis of the crops is promoted, the yield is increased, and the fruit quality is improved.
Description
Technical Field
The invention belongs to the technical field of biological fertilizer production, and particularly relates to a fish protein functional biological fertilizer and a preparation method thereof.
Background
At present, agricultural crops mostly depend on chemical fertilizers and chemical agents to improve the yield, and after long-term use, the chemical balance formed by natural microbial communities in soil is destroyed, so that the physical and chemical conditions of the soil are unbalanced, the natural fertility of the soil is seriously reduced, and more chemical fertilizers are required to be applied every year to maintain high yield, so that the cost is increased, and even the soil is completely barren and waster.
With the deep implementation of related policies such as zero growth of chemical fertilizers, ten kinds of soil and the like, various novel synergistic fertilizers have wider exertion space, and various products are accepted by broad growers by virtue of excellent application effects. The fish protein fertilizer is different from the military protuberance after the humic acid fertilizer, the seaweed fertilizer, the medium trace element fertilizer and the microbial fertilizer. The organic fertilizer can solve the negative effects brought by chemical fertilizers and chemical agents, is mainly derived from plants and/or animals, is prepared by processing carbon-containing materials which are applied to soil to provide plant nutrition as main functions of the organic fertilizer through biological substances, animal and plant wastes and plant residues, eliminates toxic and harmful substances in the organic fertilizer, is rich in a large amount of beneficial substances, and comprises: various organic acids, peptides and rich nutrient elements including nitrogen, phosphorus and potassium. The fertilizer not only can provide comprehensive nutrition for crops, but also has long fertilizer efficiency, can increase and update soil organic matters, promote microbial propagation, improve the physical and chemical properties and biological activity of soil, and is a main nutrient for green food production.
Although the organic fertilizer has more component types, the content of each nutrient is relatively less, and the organic fertilizer has more germs, needs to be stored and decomposed to kill the germs, is matched with a chemical fertilizer for use, and has better effect. The existing organic fertilizer can not meet the requirement of crops on trace elements, and the development of a fertilizer rich in organic fish protein and trace elements is particularly necessary.
Disclosure of Invention
The invention aims to solve the technical problems and provides a fish protein functional biological fertilizer and a preparation method thereof, which can convert the form of nutrient elements (slow effect state) which are difficult to utilize in soil into the form (quick effect state) which can be utilized, and increase the soil fertility.
The technical scheme of the invention is as follows: a fish protein functional biological fertilizer comprises the following components in parts by weight: 40-70 parts of fish paste, 8-20 parts of enzymolysis liquid, 1-5 parts of saccharomyces cerevisiae, 30-50 parts of urea, 10-30 parts of potassium fulvate, 30-50 parts of chitosan, 1-3 parts of ferrous sulfate, 1-3 parts of manganese sulfate, 1-3 parts of copper sulfate and 1-2 parts of molybdenum salt.
The invention also provides a preparation method of the fish protein functional biological fertilizer, which comprises the following steps:
(1) preparation of the starting Material
Beating fresh fish into slurry, stirring for 30 min, adjusting pH to 1-5.5 with acid, and mixing to obtain fish slurry;
(2) zymolysis of
A, filling fish paste into a fermentation tank with a temperature control system, adding water with the weight being 3-5 times that of the fish paste, adding enzymolysis liquid, continuously stirring, adjusting the temperature of the fermentation tank to 100-120 ℃, sterilizing for 30 minutes, cooling to 45-55 ℃, adding saccharomyces cerevisiae, fermenting for 15-20 hours, heating to 85-90 ℃, and inactivating enzyme for 20 minutes;
adding chitosan, ferrous sulfate, manganese sulfate, copper sulfate and molybdenum salt into the fish protein hydrolysate, adjusting the temperature to 60-65 ℃, and continuously stirring for reacting for 1-2 hours;
c: adding urea and potassium fulvate into the fermentation tank, and continuously stirring for reacting for 1-2 hours to obtain fish protein hydrolysate;
(3) centrifugal separation
S1, sending the fish protein hydrolysate into a flat plate type centrifuge at the rotation speed of 800-;
s2, sending the fish protein hydrolysate subjected to centrifugal separation by the centrifugal machine into a disk centrifuge to separate oil in the fish protein hydrolysate to obtain oil-free fish protein hydrolysate;
s3, filtering the oil-free fish protein hydrolysate with a 5-15 micron filter membrane to separate suspended substances in the feed liquid to obtain water-soluble fish protein hydrolysate;
s4, feeding the water-soluble fish protein hydrolysate into an ion exchange resin column for purification treatment to obtain residue-free water-soluble fish protein hydrolysate;
(4) concentrating and drying
Carrying out triple-effect vacuum evaporation on the residue-free water-soluble fish protein hydrolysate obtained after the purification treatment, and carrying out triple-effect vacuum evaporation to obtain a concentrated solution with the concentration of 45-65%; and (3) feeding the concentrated solution into a high-speed centrifugal spray dryer for drying to form ultramicro fish protein powder, wherein the water content of the ultramicro fish protein powder is less than 3%, chelating for 24 hours at 70 ℃, cooling and packaging to obtain the fish protein functional organic fertilizer.
Further, the enzymolysis solution is prepared by performing enzymatic hydrolysis on 1 of papain, neutral protease and trypsin, and the addition amount of the enzymolysis solution is 0.2-0.3% of the weight of the fish paste.
Further, in (2) A, the initial colony number of the saccharomyces cerevisiae in the water is 500-1200CFU/mL respectively.
Further, in (4), in the triple-effect vacuum evaporation stage: the temperature of the first stage is 90-95 ℃, the vacuum degree is-0.02-0.04 MPa, and the retention time of the feed liquid is 35 minutes; the temperature of the second stage is 80-85 ℃, the vacuum degree is-0.02-0.06 MPa, and the retention time of the feed liquid is 25 minutes; the temperature of the third stage is 60-65 ℃, the vacuum degree is-0.06-0.09 MPa, and the retention time of the feed liquid is 30 minutes.
Due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. after the fish protein functional biological fertilizer is applied to soil, beneficial microorganisms in the soil are rapidly propagated by taking fish protein organic substances as carriers, the activity can be improved by more than ten times, the soil structure is optimized, the fertilizer retention and water retention capacity is obviously improved, if the fish protein functional biological fertilizer is continuously used for one to several years, a large number of earthworms can be generated, the soil becomes loose and fertile, the soil ecology is improved, the form (slow effect state) of nutrient elements which are difficult to utilize in the soil can be converted into the available form (quick effect state), and the soil fertility is increased. After the fertilizer is applied to soil, the content of organic matters in the soil is greatly increased, the propagation of soil microorganisms can be rapidly promoted, the nutrients of the soil are activated to a great extent, the fertility of the soil is increased, the fertilizer and water retention capacity of the soil is improved, the soil hardening problem can be effectively improved by using the fish protein fertilizer, and the fertility of the soil is kept new.
2. The fish protein functional biological fertilizer can regulate and balance the growth and development of plants, can promote rooting when used for seed dressing and seed soaking, stimulate the growth (much, thick and long) of seedling root systems, absorb various required nutrient elements uniformly, prevent various deficiency diseases, ensure that leaves are dark green and stems are thick and strong, promote the photosynthesis of crops, promote the early blooming and fruit setting of the crops, ensure plump flower cores and fruit setting rate to be greatly improved, ensure quick fruit expansion and good color and luster, obviously increase the accumulation of nutrient substances such as protein, saccharides, vitamins and the like, effectively improve the product quality and promote the maturity of melons, fruits and vegetables. In the harvesting period, the fertilizer can keep the activity of crops, so that the harvesting period is greatly prolonged, and the fresh-keeping and storage are facilitated. Therefore, the increase of the crop yield can reach 5 to 25 percent, and the increase is different according to the crop variety, the soil and the fertilization condition.
3. The fish protein functional biological fertilizer has obvious promotion effects on the problems when the plants need to culture strong seedlings, stimulate the plant potential, expand fruits, spot color, increase sweetness and the like and when the plants suffer from stiff seedlings, yellow seedlings, no root growth, little flowers and fruits dropping and the like.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
A fish protein functional biological fertilizer comprises the following components in parts by weight: 40-70 parts of fish paste, 8-20 parts of enzymolysis liquid, 1-5 parts of saccharomyces cerevisiae, 30-50 parts of urea, 10-30 parts of potassium fulvate, 30-50 parts of chitosan, 1-3 parts of ferrous sulfate, 1-3 parts of manganese sulfate, 1-3 parts of copper sulfate and 1-2 parts of molybdenum salt.
Method 1 for preparing fish protein functional biological fertilizer
A preparation method of a fish protein functional biological fertilizer comprises the following steps:
(1) preparation of the starting Material
Beating fresh fish into slurry, stirring for 30 minutes, adjusting pH to 4.5 with acid, and mixing uniformly to obtain fish slurry;
(2) zymolysis of
A, filling fish paste into a fermentation tank with a temperature control system, adding water with the weight being 4 times that of the fish paste, adding enzymolysis liquid, continuously stirring, adjusting the temperature of the fermentation tank to 112 ℃, sterilizing for 30 minutes, cooling to 48 ℃, adding saccharomyces cerevisiae, fermenting for 18 hours, heating to 88 ℃, and inactivating enzymes for 20 minutes; wherein the initial colony number of the saccharomyces cerevisiae in water is 1000CFU/mL respectively, and the enzymolysis solution is prepared by performing enzymatic hydrolysis on 1 of papain, neutral protease and trypsin.
Adding chitosan, ferrous sulfate, manganese sulfate, copper sulfate and molybdenum salt into the fish protein hydrolysate, adjusting the temperature to 62 ℃, and continuously stirring for reaction for 1.5 hours;
c: adding urea and potassium fulvate into the fermentation tank, and continuously stirring for reacting for 1.5 hours to obtain fish protein hydrolysate;
(3) centrifugal separation
S1, conveying the fish protein hydrolysate into a flat plate type centrifuge, rotating at 1000 rpm, and centrifuging to select 150-mesh filter cloth;
s2, sending the fish protein hydrolysate subjected to centrifugal separation by the centrifugal machine into a disk centrifuge to separate oil in the fish protein hydrolysate to obtain oil-free fish protein hydrolysate;
s3, filtering the oil-free fish protein hydrolysate with a 10-micron filter membrane to separate suspended substances in the feed liquid to obtain water-soluble fish protein hydrolysate;
s4, feeding the water-soluble fish protein hydrolysate into an ion exchange resin column for purification treatment to obtain residue-free water-soluble fish protein hydrolysate;
(4) concentrating and drying
Carrying out triple-effect vacuum evaporation on the residue-free water-soluble fish protein hydrolysate obtained after the purification treatment, wherein the temperature of the first stage is 92 ℃, the vacuum degree is 0.03MPa, and the retention time of the feed liquid is 35 minutes; the temperature of the second stage is 82 ℃, the vacuum degree is 0.05MPa, and the retention time of the feed liquid is 25 minutes; the temperature of the third stage is 62 ℃, the vacuum degree is 0.07MPa, and the retention time of the feed liquid is 30 minutes. Obtaining concentrated solution with the concentration of about 50% through triple effect vacuum evaporation; and (3) feeding the concentrated solution into a high-speed centrifugal spray dryer for drying to form ultramicro fish protein powder, wherein the water content of the ultramicro fish protein powder is less than 3%, chelating for 24 hours at 70 ℃, cooling and packaging to obtain the fish protein functional organic fertilizer.
Method 2 for preparing fish protein functional biological fertilizer
A preparation method of a fish protein functional biological fertilizer comprises the following steps:
(1) preparation of the starting Material
Beating fresh fish into slurry, stirring for 30 minutes, adjusting pH to 1 with acid, and mixing uniformly to obtain fish slurry;
(2) zymolysis of
A, filling fish paste into a fermentation tank with a temperature control system, adding water with the weight being 3 times that of the fish paste, adding enzymolysis liquid, continuously stirring, adjusting the temperature of the fermentation tank to 100 ℃, sterilizing for 30 minutes, cooling to 45 ℃, adding saccharomyces cerevisiae, fermenting for 15 hours, heating to 85 ℃, and inactivating enzymes for 20 minutes; wherein the initial colony number of the saccharomyces cerevisiae in water is respectively 500CFU/mL, and the enzymolysis solution is prepared by performing enzymatic hydrolysis on 1 of papain, neutral protease and trypsin.
Adding chitosan, ferrous sulfate, manganese sulfate, copper sulfate and molybdenum salt into the fish protein hydrolysate, adjusting the temperature to 60 ℃, and continuously stirring for reaction for 1 hour;
c: adding urea and potassium fulvate into the fermentation tank, and continuously stirring for reacting for 1 hour to obtain fish protein hydrolysate;
(3) centrifugal separation
S1, conveying the fish protein hydrolysate into a flat plate type centrifuge, rotating at 800 rpm, and centrifuging to select 100-mesh filter cloth;
s2, sending the fish protein hydrolysate subjected to centrifugal separation by the centrifugal machine into a disk centrifuge to separate oil in the fish protein hydrolysate to obtain oil-free fish protein hydrolysate;
s3, filtering the oil-free fish protein hydrolysate with a 5-micron filter membrane to separate suspended substances in the feed liquid to obtain water-soluble fish protein hydrolysate;
s4, feeding the water-soluble fish protein hydrolysate into an ion exchange resin column for purification treatment to obtain residue-free water-soluble fish protein hydrolysate;
(4) concentrating and drying
Carrying out triple-effect vacuum evaporation on the residue-free water-soluble fish protein hydrolysate obtained after the purification treatment, wherein the temperature of the first stage is 90 ℃, the vacuum degree is-0.02 MPa, and the retention time of the feed liquid is 35 minutes; in the second stage, the temperature is 80 ℃, the vacuum degree is-0.02 MPa, and the retention time of the feed liquid is 25 minutes; the temperature of the third stage is 60 ℃, the vacuum degree is-0.06 MPa, and the retention time of the feed liquid is 30 minutes. Obtaining concentrated solution with the concentration of about 45 percent through triple-effect vacuum evaporation; and (3) feeding the concentrated solution into a high-speed centrifugal spray dryer for drying to form ultramicro fish protein powder, wherein the water content of the ultramicro fish protein powder is less than 3%, chelating for 24 hours at 70 ℃, cooling and packaging to obtain the fish protein functional organic fertilizer.
Method 3 for preparing fish protein functional biological fertilizer
A preparation method of a fish protein functional biological fertilizer comprises the following steps:
(1) preparation of the starting Material
Beating fresh fish into slurry, stirring for 30 minutes, adjusting pH to 5.5 with acid, and mixing uniformly to obtain fish slurry;
(2) zymolysis of
A, filling fish paste into a fermentation tank with a temperature control system, adding water with the weight 5 times that of the fish paste, adding enzymolysis liquid, continuously stirring, adjusting the temperature of the fermentation tank to 120 ℃, sterilizing for 30 minutes, cooling to 55 ℃, adding saccharomyces cerevisiae, fermenting for 20 hours, heating to 90 ℃, and inactivating enzymes for 20 minutes; wherein the initial colony number of the saccharomyces cerevisiae in water is 1200CFU/mL respectively, and the enzymolysis solution is prepared by performing enzymatic hydrolysis on 1 of papain, neutral protease and trypsin.
Adding chitosan, ferrous sulfate, manganese sulfate, copper sulfate and molybdenum salt into the fish protein hydrolysate, adjusting the temperature to 65 ℃, and continuously stirring for reacting for 2 hours;
c: adding urea and potassium fulvate into the fermentation tank, and continuously stirring for reacting for 2 hours to obtain fish protein hydrolysate;
(3) centrifugal separation
S1, conveying the fish protein hydrolysate into a flat plate type centrifuge, rotating at 1200 rpm, and centrifuging to select 200-mesh filter cloth;
s2, sending the fish protein hydrolysate subjected to centrifugal separation by the centrifugal machine into a disk centrifuge to separate oil in the fish protein hydrolysate to obtain oil-free fish protein hydrolysate;
s3, filtering the oil-free fish protein hydrolysate with a 15-micron filter membrane to separate suspended substances in the feed liquid to obtain water-soluble fish protein hydrolysate;
s4, feeding the water-soluble fish protein hydrolysate into an ion exchange resin column for purification treatment to obtain residue-free water-soluble fish protein hydrolysate;
(4) concentrating and drying
Carrying out triple-effect vacuum evaporation on the residue-free water-soluble fish protein hydrolysate obtained after the purification treatment, wherein the temperature of the first stage is 95 ℃, the vacuum degree is 0.04MPa, and the retention time of the feed liquid is 35 minutes; in the second stage, the temperature is 85 ℃, the vacuum degree is 0.06MPa, and the retention time of the feed liquid is 25 minutes; the temperature of the third stage is 65 ℃, the vacuum degree is 0.09MPa, and the retention time of the feed liquid is 30 minutes. Obtaining concentrated solution with the concentration of about 65 percent through triple-effect vacuum evaporation; and (3) feeding the concentrated solution into a high-speed centrifugal spray dryer for drying to form ultramicro fish protein powder, wherein the water content of the ultramicro fish protein powder is less than 3%, chelating for 24 hours at 70 ℃, cooling and packaging to obtain the fish protein functional organic fertilizer.
The fish protein functional biological fertilizer prepared by the method 1 is used for grapes, rice, common head cabbages, lilies and vegetables in double bridge orchard/vegetable garden experimental areas in Guangxi Wuming district, each variety is divided into 11 groups, each group comprises 10 varieties, the varieties are the same in use period, use frequency, growth environment, detection conditions (operation) and the like, the preparation methods of the fish protein functional biological fertilizer are the same, the culture technology is a technical means in the field, and the amount of the fish protein functional biological fertilizer is specifically as shown in the following table 1:
TABLE 1
In table 1, the enzymatic hydrolysates used in examples 1 to 2 and comparative examples 1 to 2 were prepared by hydrolyzing with papain, the enzymatic hydrolysates used in examples 3 to 4 and comparative examples 3 to 4 were prepared by hydrolyzing with neutral protease, and the enzymatic hydrolysates used in example 5, comparative example 5 and control were prepared by hydrolyzing with trypsin.
The results of the experiments according to table 1, using the above-mentioned functional biofertilizer for fish protein, for each variety, are shown in tables 2-6 below:
TABLE 2
From table 2, it follows: after the grapes sprout, the fish protein functional biological fertilizer is diluted by 1500 times and then sprayed, so that the growth can be accelerated, and the resistance to abnormal weather is improved; the fish protein functional biological fertilizer is diluted by 2000 times in the fruit swelling period and then sprayed, so that the yield can be obviously improved, and the sugar content can be increased.
TABLE 3
From table 3, it follows: the fish protein foliar fertilizer is sprayed once in the three-leaf one-heart, diversity period, early grouting period and middle period of the rice respectively, the rice plants are strong, the leaves are dark green, the rice ears are long, the blight rate is low and the thousand-grain weight of the rice is increased when the rice is mature. In addition, the leaves of the rice in the mature period can quickly turn color, and can mature in advance.
TABLE 4
From table 4, it follows: the fish protein functional biological fertilizer is applied in the growth period of the common head cabbage by flushing for 3 times at proper time, so that the growth and development of the common head cabbage can be promoted, and the yield can be improved.
TABLE 5
From table 5, it follows: the fish protein foliar fertilizer is sprayed once 10 days before lily picking, so that the flowering phase can be prolonged, and the flower preservation and transportation resistance are facilitated.
TABLE 6
From table 6, it follows: if the outdoor vegetables have chemical injury and fertilizer injury to cause the phenomenon of seedling stiffness, amino acid-containing fertilizers such as fish protein and the like can be applied in a flushing mode and sprayed in time to relieve the phenomenon of poor growth, so that the plant can recover the growth as soon as possible.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (5)
1. A fish protein functional biological fertilizer is characterized in that: the paint comprises the following components in parts by weight: 40-70 parts of fish paste, 8-20 parts of enzymolysis liquid, 1-5 parts of saccharomyces cerevisiae, 30-50 parts of urea, 10-30 parts of potassium fulvate, 30-50 parts of chitosan, 1-3 parts of ferrous sulfate, 1-3 parts of manganese sulfate, 1-3 parts of copper sulfate and 1-2 parts of molybdenum salt.
2. A method for preparing a fish protein functional bio-fertilizer according to claim 1, characterized in that: the method comprises the following steps:
(1) preparation of the starting Material
Beating fresh fish into slurry, stirring for 30 min, adjusting pH to 1-5.5 with acid, and mixing to obtain fish slurry;
(2) zymolysis of
A, filling fish paste into a fermentation tank with a temperature control system, adding water with the weight being 3-5 times that of the fish paste, adding enzymolysis liquid, continuously stirring, adjusting the temperature of the fermentation tank to 100-120 ℃, sterilizing for 30 minutes, cooling to 45-55 ℃, adding saccharomyces cerevisiae, fermenting for 15-20 hours, heating to 85-90 ℃, and inactivating enzyme for 20 minutes;
adding chitosan, ferrous sulfate, manganese sulfate, copper sulfate and molybdenum salt into the fish protein hydrolysate, adjusting the temperature to 60-65 ℃, and continuously stirring for reacting for 1-2 hours;
c: adding urea and potassium fulvate into the fermentation tank, and continuously stirring for reacting for 1-2 hours to obtain fish protein hydrolysate;
(3) centrifugal separation
S1, sending the fish protein hydrolysate into a flat plate type centrifuge at the rotation speed of 800-;
s2, sending the fish protein hydrolysate subjected to centrifugal separation by the centrifugal machine into a disk centrifuge to separate oil in the fish protein hydrolysate to obtain oil-free fish protein hydrolysate;
s3, filtering the oil-free fish protein hydrolysate with a 5-15 micron filter membrane to separate suspended substances in the feed liquid to obtain water-soluble fish protein hydrolysate;
s4, feeding the water-soluble fish protein hydrolysate into an ion exchange resin column for purification treatment to obtain residue-free water-soluble fish protein hydrolysate;
(4) concentrating and drying
Carrying out triple-effect vacuum evaporation on the residue-free water-soluble fish protein hydrolysate obtained after the purification treatment, and carrying out triple-effect vacuum evaporation to obtain a concentrated solution with the concentration of 45-65%; and (3) feeding the concentrated solution into a high-speed centrifugal spray dryer for drying to form ultramicro fish protein powder, wherein the water content of the ultramicro fish protein powder is less than 3%, chelating for 24 hours at 70 ℃, cooling and packaging to obtain the fish protein functional organic fertilizer.
3. A functional biological fertilizer for fish protein according to claim 1, characterized in that: the enzymolysis solution is prepared by performing enzymatic hydrolysis on 1 of papain, neutral protease and trypsin, and the addition amount of the enzymolysis solution is 0.2-0.3% of the weight of the fish paste.
4. A functional biological fertilizer for fish protein according to claim 1, characterized in that: in (2) A, the initial colony numbers of the saccharomyces cerevisiae in water are respectively 500-1200 CFU/mL.
5. A functional biological fertilizer for fish protein according to claim 1, characterized in that: in (4), the three-effect vacuum evaporation stage is as follows: the temperature of the first stage is 90-95 ℃, the vacuum degree is-0.02-0.04 MPa, and the retention time of the feed liquid is 35 minutes; the temperature of the second stage is 80-85 ℃, the vacuum degree is-0.02-0.06 MPa, and the retention time of the feed liquid is 25 minutes; the temperature of the third stage is 60-65 ℃, the vacuum degree is-0.06-0.09 MPa, and the retention time of the feed liquid is 30 minutes.
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| CN113527006A (en) * | 2021-08-16 | 2021-10-22 | 陈春喜 | Special fertilizer for efficient growth and fruit swelling period and preparation method and application thereof |
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