CN111187114A - Method for preparing liquid organic fertilizer by pigskin enzymolysis and fermentation - Google Patents
Method for preparing liquid organic fertilizer by pigskin enzymolysis and fermentation Download PDFInfo
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- CN111187114A CN111187114A CN202010258831.4A CN202010258831A CN111187114A CN 111187114 A CN111187114 A CN 111187114A CN 202010258831 A CN202010258831 A CN 202010258831A CN 111187114 A CN111187114 A CN 111187114A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Pest Control & Pesticides (AREA)
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Abstract
The invention provides a method for preparing a liquid organic fertilizer by pigskin enzymolysis and fermentation, which comprises the following steps of (1) rinsing raw pigskin with clear water until the pH value of the cleaning water is 6.5-8.0, and cutting the cleaned raw pigskin into pigskin fragments; (2) adding the pigskin fragments into a stirring tank, adding water with the same volume, adding protease, heating to 35-60 ℃, stirring for enzymolysis, adding the pigskin fragments with the same mass after complete dissolution, and continuing enzymolysis for four times to obtain polypeptide enzymolysis liquid; (3) heating the polypeptide enzymolysis liquid to 70-90 ℃ to inactivate protease, then cooling to 30-50 ℃, adding aminopeptidase, stirring and carrying out enzymolysis to obtain a primary amino acid liquid fertilizer; (4) adding a microbial agent into the primary amino acid liquid fertilizer obtained in the step 3 for fermentation and deodorization to obtain a fermentation liquid; (5) adding boric acid and zinc sulfate into the obtained fermentation liquor, dissolving, packaging, and sealing for storage. The liquid organic fertilizer prepared by the invention has high amino acid content, does not need concentration treatment and has good fertilizer efficiency.
Description
Technical Field
The invention relates to a process method for preparing an organic liquid fertilizer, in particular to a method for preparing an amino acid-containing liquid organic fertilizer by using pigskin, belonging to the technical field of amino acid-containing liquid fertilizer production processes.
Background
At present, the pigskin is an important product in the breeding industry and an important raw material in the tanning industry. About 10% of leftover materials are usually generated in the pigskin processing process, and are discarded at present, so that waste and environmental pollution are caused. The pigskin contains more collagen peptide, amino acid and other organic matters, and is a raw material for producing amino acid-containing fertilizers. The pigskin can be used for preparing the amino acid liquid fertilizer through enzymolysis, but the hydrolysis degree is low, the amino acid content in the enzymolysis liquid is low, the fertilizer efficiency is poor, the pigskin is extremely easy to rot and change odor, and the pigskin cannot directly enter the market. The concentration treatment process of the enzymolysis liquid is complex, the cost is high, the economic value is low, and the market application prospect is not met. Therefore, organic materials such as silkworm excrement, mushroom residue and the like can only be used for adsorbing the primary extract in the enzymolysis liquid, and the primary extract is sold in the form of solid organic fertilizer. However, in the finally obtained solid organic fertilizer, the proportion of organic materials such as silkworm excrement, mushroom residue and the like accounts for more than 80 percent of the finished fertilizer, and the pigskin extract accounts for only 15 to 20 percent, so that the significance and the effect of green utilization of leather waste are weakened. Therefore, a method for preparing a liquid organic fertilizer by hydrolyzing pigskin with high efficiency needs to be developed, so that the recovery rate of the pigskin is improved.
Disclosure of Invention
The invention aims to provide a method for preparing a liquid organic fertilizer by pigskin enzymolysis and fermentation, aiming at the defects of the prior art, the method can improve the pigskin enzymolysis rate and the amino acid content in the enzymolysis liquid, the product does not need to be concentrated, the process is simple, the technical process can enable the leather waste extract to account for more than 80 percent of the finished fertilizer, and the treatment and utilization capacity of the leather waste is greatly improved. The organic liquid fertilizer prepared by the technical process acts on vegetables and fruit trees, and has the effects of rich nutrition, high fertilizer efficiency, crop yield increase and crop quality improvement.
The invention provides a method for preparing a liquid organic fertilizer by pigskin enzymolysis and fermentation, which comprises the following steps:
(1) rinsing the raw pigskin with clear water until the pH value of the cleaning water is 6.5-8.0, and cutting the cleaned raw pigskin into pigskin fragments;
(2) adding the pigskin fragments into a stirring tank, adding water with the same volume, adding protease, heating to 35-60 ℃, stirring for enzymolysis, adding the pigskin fragments with the same mass after complete dissolution, and continuing enzymolysis for four times to obtain polypeptide enzymolysis liquid;
(3) heating the polypeptide enzymolysis liquid to 70-90 ℃ to inactivate protease, then cooling to 30-50 ℃, adding aminopeptidase, stirring and carrying out enzymolysis to obtain a primary amino acid liquid fertilizer;
(4) adding a microbial agent into the primary amino acid liquid fertilizer obtained in the step 3 for fermentation and deodorization to obtain a fermentation liquid;
(5) adding boric acid and zinc sulfate into the obtained fermentation liquor, dissolving, packaging, and sealing for storage.
Further, the width of the pigskin fragments is not more than 2cm, and the length of the pigskin fragments is not more than 15 cm.
Furthermore, the adding amount of the protease is 0.1-5% and the adding amount of the aminopeptidase is 1-10 per thousand based on the mass of the pigskin fragments added into the stirring tank for the first time.
Further, the rotating speed of the stirring is 50-500 rpm.
Further, in the step (4), the fermentation temperature is 25-45 ℃, and the inoculation amount of the microbial agent is 5-20kg of bacterial liquid per ton.
Further, in the step (5), the adding amount of the boric acid is 1-5% and the adding amount of the zinc sulfate is 0.2-3% by weight of the fermentation liquor.
The invention has the following beneficial effects:
according to the method for preparing the liquid organic fertilizer by pigskin enzymolysis and fermentation, provided by the invention, the pigskin is subjected to enzymolysis treatment by using protease and aminopeptidase, the pigskin enzymolysis rate can be improved, the content of amino acids in the prepared primary amino acid liquid fertilizer is high, the national standard requirements are met, concentration treatment is not required, and the treatment and utilization capacity of pigskin waste materials is greatly improved. The liquid organic fertilizer prepared by the invention acts on vegetables and fruit tree crops, and has the effects of rich nutrition, high fertilizer efficiency, crop yield increase and crop quality improvement.
Detailed Description
Exemplary embodiments that embody features and advantages of the invention are described in detail below. It is to be understood that the invention is capable of other and different embodiments and its several details are capable of modification without departing from the scope of the invention, and that the description is intended to be illustrative in nature and not to be construed as limiting the invention.
Example 1
A method for preparing a liquid organic fertilizer by pigskin enzymolysis and fermentation comprises the following steps:
(1) rinsing the raw pigskin with clear water until the pH value of the used cleaning water is 6.5, and then physically cutting the cleaned raw pigskin to prepare pigskin fragments with the width not more than 2cm and the length not more than 15 cm;
(2) adding pigskin fragments into a stirring tank, wherein the volume of the added pigskin fragments is 1/4 of the volume of the stirring tank, adding water of 1/4 of the volume of the stirring tank, adding protease with the specification of 50000u/g, the addition amount of the protease is 0.2 percent of the mass of the pigskin fragments, heating to 55 ℃, stirring for enzymolysis, adding the pigskin fragments with the same mass after the pigskin fragments are completely dissolved, continuing enzymolysis, and adding the pigskin fragments for four times in total to obtain polypeptide enzymolysis liquid;
(3) heating the polypeptide enzymolysis liquid to 70 ℃ to inactivate protease, then cooling to 35 ℃, adding aminopeptidase with the specification of 200000u/g, wherein the addition amount is 1 per mill of the mass of the pigskin fragments which are firstly added into the stirring tank, and stirring for enzymolysis for 10 hours to obtain a primary amino acid liquid fertilizer;
(4) and (3) adding a microbial agent EM into the primary amino acid liquid fertilizer obtained in the step (3), wherein the inoculation amount is 8kg of bacterial liquid/ton of polypeptide enzymolysis liquid, the concentration of the bacterial liquid is 5000-10000cFU/ml, performing fermentation deodorization at 25 ℃, and performing sealed anaerobic fermentation for 5 days to obtain fermentation liquid.
(5) Adding 1.5 wt% of boric acid and 0.8 wt% of zinc sulfate into the obtained fermentation liquor, stirring and dissolving, standing to clarify the liquid, taking supernatant, subpackaging, sealing and storing to obtain the liquid organic fertilizer.
Example 2
A method for preparing a liquid organic fertilizer by pigskin enzymolysis and fermentation comprises the following steps:
(1) rinsing the raw pigskin with clear water until the pH value of the used cleaning water is 7.2, and physically cutting the cleaned raw pigskin to prepare pigskin fragments with the width of 1cm and the length of 10 cm;
(2) adding pigskin fragments into a stirring tank, wherein the volume of the added pigskin fragments is 1/4 of the volume of the stirring tank, adding water of 1/4 of the volume of the stirring tank, adding protease with the specification of 50000u/g, the adding amount of the protease is 2 percent of the mass of the pigskin fragments, heating to 40 ℃, stirring for enzymolysis, adding the pigskin fragments with the same mass after the pigskin fragments are completely dissolved, continuing enzymolysis, and adding the pigskin fragments for four times in total to obtain polypeptide enzymolysis liquid;
(3) heating the polypeptide enzymolysis liquid to 80 ℃ to inactivate protease, then cooling to 40 ℃, adding aminopeptidase with the specification of 200000u/g, wherein the addition amount is 2.5 per mill of the mass of the pigskin fragments which are firstly added into the stirring tank, and stirring for enzymolysis for 8 hours to obtain a primary amino acid liquid fertilizer;
(4) adding brown sugar and a microbial agent EM into the primary amino acid liquid fertilizer obtained in the step 3, wherein the inoculation amount of the microbial agent is 10kg of bacterial liquid/ton of polypeptide enzymolysis liquid, the concentration of the bacterial liquid is 5000-10000cFU/ml, the addition amount of the brown sugar is 2% (m/v), the fermentation speed of the microbial agent can be improved by adding the brown sugar, the fermentation and deodorization are carried out at 38 ℃, and the anaerobic fermentation is carried out in a sealed manner for 3 days, so as to obtain the fermentation liquid.
(5) And adding 1 wt% of boric acid and 0.5 wt% of zinc sulfate into the obtained fermentation liquor, stirring and dissolving, filtering to clarify the liquid, subpackaging the obtained clear liquid, and sealing and storing to obtain the liquid organic fertilizer.
Example 3
A method for preparing a liquid organic fertilizer by pigskin enzymolysis and fermentation comprises the following steps:
(1) rinsing the raw pigskin with clear water until the pH value of the used cleaning water is 6.5, and then physically cutting the cleaned raw pigskin to prepare pigskin fragments with the width not more than 1cm and the length not more than 15 cm;
(2) adding pigskin fragments into a stirring tank, wherein the volume of the added pigskin fragments is 1/4 of the volume of the stirring tank, adding water of 1/4 of the volume of the stirring tank, adding protease of which the specification is 50000u/g, the adding amount is 5 percent of the mass of the pigskin fragments, heating to 35 ℃, stirring for enzymolysis, adding the pigskin fragments with the same mass after the pigskin fragments are completely dissolved, continuing enzymolysis, and adding the pigskin fragments for four times in total to obtain polypeptide enzymolysis liquid;
(3) heating the polypeptide enzymolysis liquid to 85 ℃ to inactivate protease, then cooling to 42 ℃, adding aminopeptidase with the specification of 200000u/g, wherein the addition amount is 6 per mill of the mass of the pigskin fragments which are firstly added into the stirring tank, and stirring for enzymolysis for 5 hours to obtain a primary amino acid liquid fertilizer;
(4) and (3) adding a microbial agent EM into the primary amino acid liquid fertilizer obtained in the step (3), wherein the inoculation amount is 15kg of bacterial liquid/ton of polypeptide enzymolysis liquid, the concentration of the bacterial liquid is 5000-10000cFU/ml, performing fermentation deodorization at 25 ℃, and performing sealed anaerobic fermentation for 6 days to obtain fermentation liquid.
(5) And adding 3 wt% of boric acid and 1.5 wt% of zinc sulfate into the obtained fermentation liquor, stirring and dissolving, standing to clarify the liquid, taking supernatant, subpackaging, sealing and storing to obtain the liquid organic fertilizer.
Comparative example 1
Experimental conditions referring to example 1, the number of volumes of the pigskin fragments used, the number of volumes of water added, and the number of masses of protease and aminopeptidase added were the same as in example 1. The difference from example 1 is that in step (2), the tank volume of the stirring tank used is 2 times of that of example 1, all pigskins are added to the stirring tank at one time, and other experimental conditions are the same as those of example 1.
Physical and chemical detection
The primary amino acid liquid fertilizers obtained in the examples of the present invention and the comparative examples were tested according to the national standard NY1429-2010 amino acid-containing water soluble fertilizer, and the results are shown in Table 1.
TABLE 1 results of physical and chemical tests
As can be seen from the above table, the pigskins of the examples and the comparative examples are subjected to effective enzymolysis, and pigskin fragments are dissolved to finally obtain uniform liquid; the pig skin fragments are added in batches, so that the content of amino acid in the primary amino acid liquid fertilizer can be obviously improved, and the product does not need to be concentrated.
Application example 1: application method and test result of applying organic liquid fertilizer prepared in example 1 to water spinach
The using method comprises the following steps:
the test field is selected from a test farm of tobacco-desk agricultural science research institute, the terrain is flat, the soil is brown, the soil is uniform, the width of a cell is 2 meters, and the length of the cell is 8 meters. The experiment was set to 3 treatments: treating 1, fermenting organic fertilizer by pigskin enzymolysis; 2, treating, namely compounding a pigskin enzymolysis fermentation organic fertilizer and a 1% seaweed fertilizer; and (3) treating with clear water ck.
Spraying 100 times of diluent for 1 time respectively in 2017, 6 and 22 months and 7 and 2 months (seedling stage of water spinach) by using an electric shoulder-carried sprayer.
The harvest was weighed at 22 days 7 months.
During the period, the water is watered for 4 times, and no fertilizer and pesticide are used.
And (3) test results:
the average cell yields of treatment 1, treatment 2 and treatment 3 are respectively: 26.32kg, 38.12kg, 22.72kg, treatment 1 and treatment 2 increased 15.84% and 23.76% respectively over the control.
Test results show that the formula of the pigskin enzymolysis and fermentation organic fertilizer, the pigskin enzymolysis and fermentation organic fertilizer and 1% of seaweed fertilizer has obvious yield increase effect on water spinach, and the pigskin enzymolysis and fermentation organic fertilizer after the seaweed fertilizer is compounded has more obvious yield increase effect. Therefore, the pigskin enzymolysis fermentation organic fertilizer is recommended to be matched with the seaweed fertilizer for use.
Application example 2: application method and test result of applying organic liquid fertilizer prepared in example 1 to apples
The method comprises the following steps:
the test field is selected in a tobacco platform to be in a Muyu-Hei-Town mud village, the terrain is flat, the land fertility is uniform, and the test time is as follows: 3-11 months in 2019. Test work: 3 # Fufu, dwarfing trees, age 7 years, 100 plants per mu.
The experiment was set to 3 treatments: treating 1, namely, cleaning water ck; treatment 2, reference fertilizer; treatment 3, product of this patent. Each zone was tested at 300 square meters and each group was replicated 3 times.
Spraying 500 times of diluent for 1 time respectively by using a shoulder-carried electric sprayer in the growing seedling stage of the apples for 4 months and 15 days, 5 months and 8 days, 6 months and 1 day, 7 months and 15 days, 8 months and 11 days and 9 months and 16 days. The fruits are harvested and weighed at 18 days after 10 months, and the fruit size and the quality indexes such as soluble solid content, Vc content, fruit water content and the like are measured. During the period, the plants are watered by drip irrigation for 12 times and the weeds are removed for 5 times. 800kg of fermented cow dung and 100kg of compound fertilizer of nitrogen, phosphorus and potassium (15-15-15) are used per mu before planting, and other chemical fertilizers and pesticides are not used in the growing period.
The application effect of each test treatment is shown in table 2. The yield of the product in the small district is 885 kg, and is increased by 29.2 percent and 8.7 percent respectively compared with 685 kg of clear water and 814 kg of reference fertilizer. While increasing the yield, the content of Vc soluble solids is respectively increased by 26.1 percent and 23.3 percent compared with the content of control and reference fertilizers; soluble sugar solids content increased 14.1% and 6.2% over control and reference fertilizer treatments, respectively.
This patent fertilizer can promote the inflation of apple fruit (see table 3), and the obvious contrast of little fruit (less than or equal to 75 mm) rate reduces with reference fertilizer group, and big fruit (more than or equal to 85 mm) is handled and is referred to fertilizer group and increase than the contrast. The individual fruit weight was increased by 12.3% and 6.2% respectively over the control and reference fertilizer treatments.
The test result shows that the product of the patent has obvious yield increase and quality improvement on Fuji apples.
TABLE 2 Effect of different fertilizers on apple yield and quality
TABLE 3 Effect of different fertilizers on apple fruit size
Unless otherwise defined, all terms used herein have the meanings commonly understood by those skilled in the art.
The described embodiments of the present invention are for illustrative purposes only and are not intended to limit the scope of the present invention, and various other substitutions, changes and modifications may be made by those skilled in the art within the scope of the present invention, and thus, various changes and modifications may be made by those skilled in the art to the technical solution of the present invention without departing from the spirit of the present invention, which is defined by the claims.
Claims (6)
1. A method for preparing a liquid organic fertilizer by pigskin enzymolysis and fermentation is characterized by comprising the following steps:
(1) rinsing the raw pigskin with clear water until the pH value of the cleaning water is 6.5-8.0, and cutting the cleaned raw pigskin into pigskin fragments;
(2) adding the pigskin fragments into a stirring tank, adding water with the same volume, adding protease, heating to 35-60 ℃, stirring for enzymolysis, adding the pigskin fragments with the same mass after complete dissolution, and continuing enzymolysis for four times to obtain polypeptide enzymolysis liquid;
(3) heating the polypeptide enzymolysis liquid to 70-90 ℃ to inactivate protease, then cooling to 30-50 ℃, adding aminopeptidase, stirring and carrying out enzymolysis to obtain a primary amino acid liquid fertilizer;
(4) adding a microbial agent into the primary amino acid liquid fertilizer obtained in the step 3 for fermentation and deodorization to obtain a fermentation liquid;
(5) adding boric acid and zinc sulfate into the obtained fermentation liquor, dissolving, packaging, and sealing for storage.
2. The method for preparing the liquid organic fertilizer through the enzymolysis and fermentation of the pigskin according to claim 1, wherein the width of the pigskin fragments is not more than 2cm, and the length of the pigskin fragments is not more than 15 cm.
3. The method for preparing the liquid organic fertilizer through the enzymolysis and fermentation of the pigskin according to claim 1, wherein the adding amount of the protease is 0.1-5% and the adding amount of the aminopeptidase is 1-10% by mass based on the mass of the pigskin fragments added into the stirring tank for the first time.
4. The method for preparing the liquid organic fertilizer through the pigskin enzymolysis and fermentation according to claim 1, wherein the stirring rotating speed is 50-500 rpm.
5. The method for preparing the liquid organic fertilizer through the pigskin enzymolysis and fermentation according to claim 1, wherein in the step (4), the fermentation temperature is 25-45 ℃, and the inoculation amount of the microbial agent is 5-20kg of bacterial liquid per ton.
6. The method for preparing the liquid organic fertilizer by the enzymolysis and fermentation of the pigskin according to claim 1, wherein in the step (5), the addition amount of the boric acid is 1-5% and the addition amount of the zinc sulfate is 0.2-3% based on the weight of the fermentation liquor.
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| CN202010258831.4A CN111187114A (en) | 2020-04-03 | 2020-04-03 | Method for preparing liquid organic fertilizer by pigskin enzymolysis and fermentation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115417735A (en) * | 2022-09-29 | 2022-12-02 | 甘肃省农业科学院旱地农业研究所 | Synergistic fertilizer and preparation method thereof |
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| CN109321624A (en) * | 2018-11-15 | 2019-02-12 | 安徽嘉润生物科技有限责任公司 | A kind of enzymolysis process of fresh porcine skin preparation active collagen peptide |
| CN110734885A (en) * | 2019-12-06 | 2020-01-31 | 安徽瑞驰兰德生物科技有限公司 | composite microbial agent for fermenting trash fish and preparation method of enzymolysis fish protein amino acid water-soluble fertilizer |
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2020
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102911991A (en) * | 2012-09-28 | 2013-02-06 | 湖州佳美生物化学制品有限公司 | Method for extracting low molecular weight active collagen peptide from pigskin |
| RO131871A2 (en) * | 2015-11-18 | 2017-05-30 | Institutul Naţional De Cercetare-Dezvoltare Pentru Textile Şi Pielărie - Sucursala Institutul De Cercetare Pielărie | Collagen polydispersions for treating cereal seeds and process for preparing the same |
| CN105753571A (en) * | 2016-02-25 | 2016-07-13 | 山东省烟台市农业科学研究院 | Process method for preparing organic liquid fertilizer by using leather production lime plte wastes |
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Application publication date: 20200522 |