CN102911991A - Method for extracting low molecular weight active collagen peptide from pigskin - Google Patents

Method for extracting low molecular weight active collagen peptide from pigskin Download PDF

Info

Publication number
CN102911991A
CN102911991A CN2012103677857A CN201210367785A CN102911991A CN 102911991 A CN102911991 A CN 102911991A CN 2012103677857 A CN2012103677857 A CN 2012103677857A CN 201210367785 A CN201210367785 A CN 201210367785A CN 102911991 A CN102911991 A CN 102911991A
Authority
CN
China
Prior art keywords
pigskin
enzymolysis
collagen peptide
protease
molecular weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2012103677857A
Other languages
Chinese (zh)
Inventor
俞继荣
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HUZHOU JIAMEI BIOCHEMICAL PRODUCTS CO Ltd
Original Assignee
HUZHOU JIAMEI BIOCHEMICAL PRODUCTS CO Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HUZHOU JIAMEI BIOCHEMICAL PRODUCTS CO Ltd filed Critical HUZHOU JIAMEI BIOCHEMICAL PRODUCTS CO Ltd
Priority to CN2012103677857A priority Critical patent/CN102911991A/en
Publication of CN102911991A publication Critical patent/CN102911991A/en
Pending legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to the technical field of deep processing of pigskin, particularly relates to a method for extracting pigskin collagen peptide. The method uses fresh pigskin to serve as a raw material, and a pure biology method is used for obtaining the pigskin collagen peptide. The method for extracting low molecular weight active collagen peptide from the pigskin comprises the following steps of: taking the fresh pigskin to serve as the raw material, firstly conducting outer cutting on collagen in the pigskin by adopting aminopeptidase and carboxypeptidase; separating the collagen from other tissue elements in the pigskin, then the pigskin is in a scattered state, and using endo protease to conduct enzymolysis on protein to enable the protein to form micromolecular protein peptides. Different protein peptides are scientifically selected in different stages, processing steps are optimized, production efficiency is improved, yield of the collagen peptide is promoted, and activity of the collagen peptide is improved.

Description

From pigskin, extract the method for lower molecular weight active collagen peptide
Technical field
The inventive method relates to the deep process technology field of pigskin, is specifically related to a kind of extracting method of pigskin collagen peptides, is a kind of take fresh porcine skin as raw material, utilizes pure biological means to obtain the method for pigskin collagen peptides.
Background technology
About moisture content 70%～75% in the pigskin, protein 21%～23%, fat 1%, ash content 0.5%.Protein in the pigskin is take collagen protein as main, and content is 87.8%.Collagen protein has three strands of unique superhelixes, article three, chain is parallel to each other and is linked to each other by interchain hydrogen bond, has very stable character, and general processing temperature and short period of time heat all that difficulty makes its decomposition, therefore difficulty is absorbed by the body, and directly edible utilization ratio is lower; After collagen hydrolysate is polypeptide; digestibility almost reaches 100%; and its nutrition and physiological function also can significantly improve; as: protection gastric mucosa and antiulcer agent; promote the skin collagen metabolism; suppress increased blood pressure, can promote calcium absorption and reduce cholesterol in serum content, the collagenosiss such as sacroiliitis are also all had good prevention and therapeutic action.In recent years, the development and utilization of collagen protein had caused that people paid close attention to widely.But, be not that all collagen peptides can both be applied to different fields easily.The purposes of collagen peptide depends primarily on the molecular weight of collagenic hydrolysate, and the application of collagen peptide in medicine, food, makeup, fine chemical product is all relevant with its molecular weight.The size of molecular weight has become the standard of weighing quality product.
At present, the collagen peptide of domestic production, its molecular weight is many more than 2000 dalton, and biologic activity is very low.It is 201110315438.5 Chinese invention patent such as application number, a kind of extracting method of pigskin collagen peptides is disclosed, specifically comprise the steps: 1) take by weighing fresh porcine skin as raw material, after clean with 35 ℃～45 ℃ water, be cut into the rectangular of 1～3cm with dicer, rub with mincer again, again by raw material weight 6～9 extraordinarily purified water stir; 2) then be warming up to 50～55 ℃, regulate pH value to 7～8.5, the proteolytic enzyme that adds the 22U/mg of raw material pigskin weight 1～2%, temperature remains on 50～55 ℃, reaction 4～6h, keep system pH value stabilization in the reaction process, after enzymolysis is complete the enzymolysis solution that obtains is warming up to 90～95 ℃, keep 10～15min enzyme that goes out; 3) add again the flavor protease of the 28U/mg of raw material pigskin weight 0.5～1%, stir enzymolysis 3～4h, keep pH value 6.5～8 in the enzymolysis process, after enzymolysis is complete the enzymolysis solution that obtains is warming up to 90～95 ℃, maintenance 10～15min enzyme that goes out; 4) with enzymolysis solution with the centrifugal fat of sloughing of whizzer, be protein liquid; 5) will add the powdered active carbon decolouring of raw material pigskin weight 1.8～2.2% in the protein liquid, then the filtering gac gets filtrate; 6) filtrate is separated through the nanofiltration separation system again, and desalination is also concentrated; 7) concentrated solution namely gets the pigskin collagen peptides dry product after drying.The method adopts the technology of twice enzymolysis, complex manufacturing, and the fat that adopts whizzer to slough is particulate state, and fat particle is also remaining a collagen protein, and the yield of collagen peptide is low, and biologic activity is low.
Summary of the invention
The purpose of this invention is to provide a kind of molecular weight distribution of producing in the method for 500～2000 collagen peptide.
Above-mentioned technical purpose of the present invention is achieved by the following technical programs:
From pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
Figure 2012103677857100002DEST_PATH_IMAGE001
Take fresh porcine skin as raw material, be cut into cutlet after cleaning, add the deionized water of 5～10 times of amounts of raw materials quality;
Be warming up to 40～50 ℃, add the compound protease of raw materials quality 2 ‰～5 ‰
Figure 2012103677857100002DEST_PATH_IMAGE003
, carry out enzymolysis;
Figure 452446DEST_PATH_IMAGE004
Through compound protease
Figure 169866DEST_PATH_IMAGE003
Processing, when cutlet disintegrates when becoming loose shape, add the compound protease of raw materials quality 2 ‰～5 ‰
Figure 2012103677857100002DEST_PATH_IMAGE005
, further enzymolysis;
Figure 565075DEST_PATH_IMAGE006
After enzymolysis is complete, be warming up to 90～95 ℃, keep with enzyme-deactivating, leaving standstill more than the 15min, treat the complete layering of enzymolysis solution and lipid layer, isolate the limpid liquid of lower floor;
Figure 2012103677857100002DEST_PATH_IMAGE007
In separating the liquid that flows out, add gac, filter after the decolouring, get filtrate;
Figure 566398DEST_PATH_IMAGE008
Filtrate is used the ultra-filtration equipment filtering and concentrating, and concentrated solution namely gets lower molecular weight active collagen peptide dry product after drying;
Described prozyme It is the mixing protease that comprises aminopeptidase, carboxypeptidase and endo-protease;
Described prozyme
Figure 593577DEST_PATH_IMAGE005
It is endo-protease.
Preferred as technique scheme, step Described drying is spraying drying.
The present invention has following technique effect:
The present invention does not add any chemical substance, environmental protection except adding enzyme.The present invention adopts two step enzyme cutting methods to extract collagen peptide from pigskin, has advantages of that Product Activity is high, yield is high.Aminopeptidase and carboxypeptidase are excision enzymes, the present invention at first adopts aminopeptidase and carboxypeptidase to carry out circumscribed to the collagen protein in the pigskin, other structural constituents of collagen protein and pigskin are separated, after making pigskin become loose shape, with endo-protease protein is carried out enzymolysis again and make protein become micromolecular protein peptide.Nearly all adopted go out for twice technique of enzyme of twice enzymolysis in the prior art, it is 201110315438.5 Chinese invention patent such as application number, the enzyme that goes out for twice all needs to improve temperature, then cooling, not only process trouble, and temperature is a very important factor in order for enzymic activity, and the control of temperature thing easily, therefore go out for the twice technological process operation easier of enzyme of twice enzymolysis is larger in the prior art, is unfavorable for the raising of production efficiency.And the present invention adopts two step enzymolysis, and the technique of the enzyme that once goes out has been saved the enzyme operation of going out, and effectively promoted production efficiency, and because enzyme of the present invention is chosen science, the yield of collagen peptide is also high than prior art.In the prior art, through behind the enzymolysis, the fat in the enzymolysis solution is particulate state; form suspension with enzymolysis solution, with centrifugal effect and not obvious, even delamination; and because fat particle is also remaining collagen protein being arranged, yield is very low, and the biologic activity of collagen peptide is also very low.And the present invention can thoroughly solve the problems of the technologies described above, behind the process enzymolysis second time, fat no longer is particulate state but becomes fat liquor, all fat almost all become liquid, by leaving standstill, fat liquor and enzymolysis solution are realized effective layering, and fat liquor swims on the enzymolysis solution, in this case, isolate enzymolysis solution and except degrease very convenient.Why adopt in the prior art enzyme that goes out for twice be because, be difficult to judge whether enzymolysis is complete for the first time, if enzymolysis is incomplete for the first time, the enzyme enzymolysis that the enzyme that adds so for the second time probably will add for the first time falls, the enzyme enzymolysis that the enzyme that perhaps adds is for the second time added for the first time, that is, there is the possibility of mutual enzymolysis between the different enzymes, causes the enzymolysis failure.And among the present invention, the enzyme that adds for the first time is aminopeptidase, carboxypeptidase and endo-protease, adopts aminopeptidase, carboxypeptidase and endo-protease that the collagen protein in the pigskin is carried out enzyme and cuts, when enzymolysis is complete, pigskin becomes loose shape, and namely other structural constituents of collagen protein and pigskin are separated.Add again the second enzyme this moment, the phenomenon of mutual enzymolysis between the different enzymes just can not occur.Therefore the present invention is achieved the technical scheme of the enzyme that do not go out in the two step enzymolysis process, and this technical scheme has significant technical progress with respect to prior art.
In sum, the present invention has scientifically chosen different proteolytic enzyme in different steps, has optimized processing step, has improved production efficiency, has promoted the yield of collagen peptide, has improved the activity of collagen peptide.The product that adopts the present invention to make, molecular weight account for approximately 90% between 500～2000 dalton, on average about 1000 dalton, active polypeptide content is up to more than 85%.
Embodiment
Following specific embodiment only is the explanation to the application; be not the restriction to the application, those skilled in the art behind the specification sheets of having read the application, any modification that the application is carried out; as long as in the scope of claims, all will be subject to the protection of law.
Embodiment one
From pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
1. from pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
Figure 648307DEST_PATH_IMAGE001
Take fresh porcine skin as raw material, be cut into cutlet after cleaning, add the deionized water of 5 times of amounts of raw materials quality;
Figure 641670DEST_PATH_IMAGE002
Be warming up to 40 ℃, add the compound protease of raw materials quality 2 ‰
Figure 396000DEST_PATH_IMAGE003
, carry out enzymolysis;
Through compound protease Processing, when cutlet disintegrates when becoming loose shape, add the compound protease of raw materials quality 5 ‰
Figure 210875DEST_PATH_IMAGE005
, further enzymolysis;
Figure 452500DEST_PATH_IMAGE006
After enzymolysis is complete, be warming up to 90～95 ℃, keep with enzyme-deactivating, leaving standstill more than the 15min, treat the complete layering of enzymolysis solution, isolate the limpid liquid of lower floor;
Figure 727624DEST_PATH_IMAGE007
In separating the liquid that flows out, add gac, filter after the decolouring, get filtrate;
Figure 308778DEST_PATH_IMAGE008
Filtrate is used the ultra-filtration equipment filtering and concentrating, and concentrated solution namely gets lower molecular weight active collagen peptide dry product after drying;
Described prozyme
Figure 643944DEST_PATH_IMAGE003
It is the mixing protease that comprises aminopeptidase, carboxypeptidase and endo-protease;
Described prozyme
Figure 372866DEST_PATH_IMAGE005
It is endo-protease;
Described aminopeptidase is the subtilis aminopeptidase, and described carboxypeptidase is ox pancreas carboxypeptidase, and described endo-protease is stomach en-.
Embodiment two
From pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
1. from pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
Figure 653280DEST_PATH_IMAGE001
Take fresh porcine skin as raw material, be cut into cutlet after cleaning, add the deionized water of 7 times of amounts of raw materials quality;
Figure 151257DEST_PATH_IMAGE002
Be warming up to 42 ℃, add the compound protease of raw materials quality 3 ‰ , carry out enzymolysis;
Figure 811225DEST_PATH_IMAGE004
Through compound protease
Figure 693731DEST_PATH_IMAGE003
Processing, when cutlet disintegrates when becoming loose shape, add the compound protease of raw materials quality 4 ‰
Figure 46215DEST_PATH_IMAGE005
, further enzymolysis;
Figure 175714DEST_PATH_IMAGE006
After enzymolysis is complete, be warming up to 90～95 ℃, keep with enzyme-deactivating, leaving standstill more than the 15min, treat the complete layering of enzymolysis solution, isolate the limpid liquid of lower floor;
Figure 613648DEST_PATH_IMAGE007
In separating the liquid that flows out, add gac, filter after the decolouring, get filtrate;
Figure 34265DEST_PATH_IMAGE008
Filtrate is used the ultra-filtration equipment filtering and concentrating, and concentrated solution namely gets lower molecular weight active collagen peptide dry product after drying;
Described prozyme
Figure 444518DEST_PATH_IMAGE003
It is the mixing protease that comprises aminopeptidase, carboxypeptidase and endo-protease;
Described prozyme It is endo-protease;
Described aminopeptidase is the subtilis aminopeptidase, and described carboxypeptidase is pig pancreas carboxypeptidase, and described endo-protease is papoid.
Embodiment three
1. from pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
Figure 670149DEST_PATH_IMAGE001
Take fresh porcine skin as raw material, be cut into cutlet after cleaning, add the deionized water of 8 times of amounts of raw materials quality;
Figure 894457DEST_PATH_IMAGE002
Be warming up to 45 ℃, add the compound protease of raw materials quality 4 ‰
Figure 955954DEST_PATH_IMAGE003
, carry out enzymolysis;
Figure 177988DEST_PATH_IMAGE004
Through compound protease
Figure 590514DEST_PATH_IMAGE003
Processing, when cutlet disintegrates when becoming loose shape, add the compound protease of raw materials quality 3 ‰
Figure 618513DEST_PATH_IMAGE005
, further enzymolysis;
Figure 987047DEST_PATH_IMAGE006
After enzymolysis is complete, be warming up to 90～95 ℃, keep with enzyme-deactivating, leaving standstill more than the 15min, treat the complete layering of enzymolysis solution, isolate the limpid liquid of lower floor;
Figure 176720DEST_PATH_IMAGE007
In separating the liquid that flows out, add gac, filter after the decolouring, get filtrate;
Filtrate is used the ultra-filtration equipment filtering and concentrating, and concentrated solution namely gets lower molecular weight active collagen peptide dry product after drying;
Described prozyme
Figure 580336DEST_PATH_IMAGE003
It is the mixing protease that comprises aminopeptidase, carboxypeptidase and endo-protease;
Described prozyme
Figure 882004DEST_PATH_IMAGE005
It is endo-protease.
Described aminopeptidase is the aspergillus oryzae aminopeptidase, and described carboxypeptidase is people's pancreas carboxypeptidase, and described endo-protease is the mixed enzyme of stomach en-and papoid.
Embodiment four
1. from pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
Figure 432459DEST_PATH_IMAGE001
Take fresh porcine skin as raw material, be cut into cutlet after cleaning, add the deionized water of 10 times of amounts of raw materials quality;
Be warming up to 50 ℃, add the compound protease of raw materials quality 5 ‰
Figure 189379DEST_PATH_IMAGE003
, carry out enzymolysis;
Figure 17658DEST_PATH_IMAGE004
Through compound protease
Figure 814713DEST_PATH_IMAGE003
Processing, when cutlet disintegrates when becoming loose shape, add the compound protease of raw materials quality 2 ‰
Figure 423549DEST_PATH_IMAGE005
, further enzymolysis;
Figure 783992DEST_PATH_IMAGE006
After enzymolysis is complete, be warming up to 90～95 ℃, keep with enzyme-deactivating, leaving standstill more than the 15min, treat the complete layering of enzymolysis solution, isolate the limpid liquid of lower floor;
Figure 794673DEST_PATH_IMAGE007
In separating the liquid that flows out, add gac, filter after the decolouring, get filtrate;
Figure 762629DEST_PATH_IMAGE008
Filtrate is used the ultra-filtration equipment filtering and concentrating, and concentrated solution namely gets lower molecular weight active collagen peptide dry product after drying;
Described prozyme
Figure 796444DEST_PATH_IMAGE003
It is the mixing protease that comprises aminopeptidase, carboxypeptidase and endo-protease;
Described prozyme
Figure 508048DEST_PATH_IMAGE005
It is endo-protease.
Described aminopeptidase is the subtilis aminopeptidase, and described carboxypeptidase is pig pancreas carboxypeptidase, and described endo-protease is the mixed enzyme of stomach en-and papoid.
Comparative Examples
From pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
Figure 373236DEST_PATH_IMAGE001
Take by weighing fresh porcine skin as raw material, after cleaning with 40 ℃ of water, be cut into the rectangular of 1～3cm with dicer, rub with mincer again, again by raw material weight 7 extraordinarily purified water stir;
Figure 699044DEST_PATH_IMAGE002
Then be warming up to 52 ℃, regulate pH value to 8, add the proteolytic enzyme of the 22U/mg of raw material pigskin weight 2%, temperature remains on 52 ℃, and reaction 4h keeps system pH value stabilization in the reaction process, after enzymolysis is complete the enzymolysis solution that obtains is warming up to 90～95 ℃, keeps 10～15min enzyme that goes out;
Figure 282472DEST_PATH_IMAGE004
The flavor protease that adds again the 28U/mg of raw material pigskin weight 1% stirs enzymolysis 3h, keeps pH value 8 in the enzymolysis process, after enzymolysis is complete the enzymolysis solution that obtains is warming up to 90～95 ℃, maintenance 10～15min enzyme that goes out;
Figure 469871DEST_PATH_IMAGE006
Enzymolysis solution with the centrifugal fat of sloughing of whizzer, is protein liquid;
Figure 455145DEST_PATH_IMAGE007
To add the powdered active carbon decolouring of raw material pigskin weight 2% in the protein liquid, then the filtering gac gets filtrate;
Figure 764903DEST_PATH_IMAGE008
Filtrate is separated through the nanofiltration separation system again, and desalination is also concentrated, and concentrated solution namely gets the pigskin collagen peptides dry product after drying.
Figure 22578DEST_PATH_IMAGE010

Claims (2)

1. from pigskin, extract the method for lower molecular weight active collagen peptide, may further comprise the steps:
Figure 2012103677857100001DEST_PATH_IMAGE002
Take fresh porcine skin as raw material, be cut into cutlet after cleaning, add the deionized water of 5～10 times of amounts of raw materials quality;
Figure 2012103677857100001DEST_PATH_IMAGE004
Be warming up to 40～50 ℃, add the compound protease of raw materials quality 2 ‰～5 ‰
Figure 2012103677857100001DEST_PATH_IMAGE006
, carry out enzymolysis;
Figure 2012103677857100001DEST_PATH_IMAGE008
Through compound protease
Figure 453076DEST_PATH_IMAGE006
Processing, when cutlet disintegrates when becoming loose shape, add the compound protease of raw materials quality 2 ‰～5 ‰
Figure 2012103677857100001DEST_PATH_IMAGE010
, further enzymolysis;
After enzymolysis is complete, be warming up to 90～95 ℃, keep with enzyme-deactivating, leaving standstill more than the 15min, treat the complete layering of enzymolysis solution and lipid layer, isolate the limpid liquid of lower floor;
Figure DEST_PATH_IMAGE014
In isolated liquid, add gac, filter after the decolouring, get filtrate;
Figure DEST_PATH_IMAGE016
Filtrate is used the ultra-filtration equipment filtering and concentrating, and concentrated solution namely gets lower molecular weight active collagen peptide dry product after drying;
Described prozyme
Figure 738258DEST_PATH_IMAGE006
It is the mixing protease that comprises aminopeptidase, carboxypeptidase and endo-protease;
Described prozyme
Figure 517995DEST_PATH_IMAGE010
It is endo-protease.
2. a kind of method of extracting low-molecular weight collagen peptide from pigskin according to claim 1 is characterized in that: step
Figure 850888DEST_PATH_IMAGE016
Described drying is spraying drying.
CN2012103677857A 2012-09-28 2012-09-28 Method for extracting low molecular weight active collagen peptide from pigskin Pending CN102911991A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2012103677857A CN102911991A (en) 2012-09-28 2012-09-28 Method for extracting low molecular weight active collagen peptide from pigskin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN2012103677857A CN102911991A (en) 2012-09-28 2012-09-28 Method for extracting low molecular weight active collagen peptide from pigskin

Publications (1)

Publication Number Publication Date
CN102911991A true CN102911991A (en) 2013-02-06

Family

ID=47610568

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2012103677857A Pending CN102911991A (en) 2012-09-28 2012-09-28 Method for extracting low molecular weight active collagen peptide from pigskin

Country Status (1)

Country Link
CN (1) CN102911991A (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103305580A (en) * 2013-07-03 2013-09-18 广州金娜宝生物科技有限公司 Method for preparing collagen peptide from mammal skin and bone
CN103947819A (en) * 2014-04-30 2014-07-30 桂林军供生化技术开发有限公司 Method for extracting protein from pigskin
CN103952457A (en) * 2014-04-30 2014-07-30 桂林军供生化技术开发有限公司 Method for extracting active collagen peptide with low molecular weight from chicken skin
CN104946711A (en) * 2014-03-27 2015-09-30 苏州吉利鼎海洋生物科技有限公司 Production process of collagen
CN105969830A (en) * 2016-01-26 2016-09-28 成都市科乐生物技术有限公司 Method for extracting active collagen peptide from pigskin
CN106367461A (en) * 2016-08-26 2017-02-01 湖州佳美生物化学制品有限公司 Method for extracting low molecular weight active collagen peptide from pigskins
CN107041467A (en) * 2017-03-31 2017-08-15 上海优久生物科技有限公司 A kind of pig split silk forage protein processing method and its application
CN107683995A (en) * 2017-07-10 2018-02-13 杨宇 A kind of short peptide type solid beverage
CN110151566A (en) * 2019-05-20 2019-08-23 上海应用技术大学 A kind of skin care freeze-dried powder of the polypeptide containing pigskin collagen and its preparation
CN111187114A (en) * 2020-04-03 2020-05-22 山东省烟台市农业科学研究院 Method for preparing liquid organic fertilizer by pigskin enzymolysis and fermentation
CN111548409A (en) * 2020-05-21 2020-08-18 内蒙古元本生物医药科技有限公司 Extraction process of animal fresh skin collagen polypeptide
CN115417735A (en) * 2022-09-29 2022-12-02 甘肃省农业科学院旱地农业研究所 Synergistic fertilizer and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1683550A (en) * 2005-03-08 2005-10-19 国家海洋局第一海洋研究所 Collagen and its preparing process
CN101074436A (en) * 2006-05-15 2007-11-21 上海承润生物科技发展有限公司 Special composite pankrin for extracting animal collagen and amino acid
CN102367464A (en) * 2011-10-18 2012-03-07 得利斯集团有限公司 Method for extracting pigskin collagen peptide

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1683550A (en) * 2005-03-08 2005-10-19 国家海洋局第一海洋研究所 Collagen and its preparing process
CN101074436A (en) * 2006-05-15 2007-11-21 上海承润生物科技发展有限公司 Special composite pankrin for extracting animal collagen and amino acid
CN102367464A (en) * 2011-10-18 2012-03-07 得利斯集团有限公司 Method for extracting pigskin collagen peptide

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
臧盛等: "猪皮胶原蛋白的研究进展", 《农产品加工.创新版》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103305580A (en) * 2013-07-03 2013-09-18 广州金娜宝生物科技有限公司 Method for preparing collagen peptide from mammal skin and bone
CN104946711A (en) * 2014-03-27 2015-09-30 苏州吉利鼎海洋生物科技有限公司 Production process of collagen
CN103947819A (en) * 2014-04-30 2014-07-30 桂林军供生化技术开发有限公司 Method for extracting protein from pigskin
CN103952457A (en) * 2014-04-30 2014-07-30 桂林军供生化技术开发有限公司 Method for extracting active collagen peptide with low molecular weight from chicken skin
CN105969830A (en) * 2016-01-26 2016-09-28 成都市科乐生物技术有限公司 Method for extracting active collagen peptide from pigskin
CN106367461A (en) * 2016-08-26 2017-02-01 湖州佳美生物化学制品有限公司 Method for extracting low molecular weight active collagen peptide from pigskins
CN107041467A (en) * 2017-03-31 2017-08-15 上海优久生物科技有限公司 A kind of pig split silk forage protein processing method and its application
CN107683995A (en) * 2017-07-10 2018-02-13 杨宇 A kind of short peptide type solid beverage
CN110151566A (en) * 2019-05-20 2019-08-23 上海应用技术大学 A kind of skin care freeze-dried powder of the polypeptide containing pigskin collagen and its preparation
CN111187114A (en) * 2020-04-03 2020-05-22 山东省烟台市农业科学研究院 Method for preparing liquid organic fertilizer by pigskin enzymolysis and fermentation
CN111548409A (en) * 2020-05-21 2020-08-18 内蒙古元本生物医药科技有限公司 Extraction process of animal fresh skin collagen polypeptide
CN115417735A (en) * 2022-09-29 2022-12-02 甘肃省农业科学院旱地农业研究所 Synergistic fertilizer and preparation method thereof

Similar Documents

Publication Publication Date Title
CN102911991A (en) Method for extracting low molecular weight active collagen peptide from pigskin
US10455849B2 (en) Method for the preparation of a protein peptide, a protein peptide and use thereof
US10414795B2 (en) Techniques of preparing collagen active peptides
CN108998488A (en) A kind of activity royal jelly OCO polypeptide freeze-dried powder and preparation method thereof
JP5734101B2 (en) Oyster extract manufacturing method and oyster extract
CN105969830A (en) Method for extracting active collagen peptide from pigskin
CN102766670A (en) Preparation method of oyster polypeptide powder
JP2007068454A (en) Method for producing rice protein, rice protein produced by the method, and food
CN103343153A (en) Method for preparing forest frog oil peptides by enzymatic hydrolysis and forest frog oil peptides
CN110178964A (en) The preparation process of sturgeon protein peptides
JP5027001B2 (en) Enzyme-treated royal jelly and skin fibroblast growth promoter containing the same
CN102228125B (en) Preparation method of algal active peptide
CN106174436A (en) A kind of preparation method of complex enzyme hydrolysis fishbone hormone
CN105821106A (en) Method for preparing sea cucumber small molecular peptide
CN106359839A (en) Extraction method of oyster peptides
CN106367461A (en) Method for extracting low molecular weight active collagen peptide from pigskins
CN105433107A (en) A preparing method of a lobster extract
CN106480145A (en) A kind of extracting method of small molecule oyster polypeptide
CN103952457A (en) Method for extracting active collagen peptide with low molecular weight from chicken skin
KR101021645B1 (en) The making methode of whitened peptide from squid collagen
CN102277405A (en) Preparation method of snakeskin active peptide
CN101130801A (en) Preparation of active antihypertensive peptide product
CN105996057A (en) Fruit and egg white peptide tablets and preparing method thereof
Iltchenco et al. Profiles of enzymatic hydrolysis of different collagens and derivatives over time
JP3691497B2 (en) Production of amino acid components by enzymatic degradation of fish egg shells

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20130206