CN115474459A - Method for promoting germination of polygonatum sibiricum seeds - Google Patents
Method for promoting germination of polygonatum sibiricum seeds Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 65
- 230000035784 germination Effects 0.000 title claims abstract description 53
- 241000037831 Polygonatum sibiricum Species 0.000 title claims abstract description 50
- 230000001737 promoting effect Effects 0.000 title claims abstract description 34
- 238000002791 soaking Methods 0.000 claims abstract description 62
- 230000005059 dormancy Effects 0.000 claims abstract description 55
- 229930191978 Gibberellin Natural products 0.000 claims abstract description 36
- 239000003448 gibberellin Substances 0.000 claims abstract description 36
- 239000000758 substrate Substances 0.000 claims abstract description 36
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000003630 growth substance Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 57
- 238000007789 sealing Methods 0.000 claims description 32
- 235000013399 edible fruits Nutrition 0.000 claims description 18
- 238000001035 drying Methods 0.000 claims description 17
- 239000012535 impurity Substances 0.000 claims description 17
- 238000003892 spreading Methods 0.000 claims description 17
- 230000007480 spreading Effects 0.000 claims description 17
- 238000003860 storage Methods 0.000 claims description 4
- AKOVXPNRITVXAR-UHFFFAOYSA-N 2-[(7h-purin-6-ylamino)methyl]phenol Chemical compound OC1=CC=CC=C1CNC1=NC=NC2=C1NC=N2 AKOVXPNRITVXAR-UHFFFAOYSA-N 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 36
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 230000007226 seed germination Effects 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 29
- 238000012360 testing method Methods 0.000 description 14
- 210000001161 mammalian embryo Anatomy 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical class C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 4
- 241000756042 Polygonatum Species 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000005556 hormone Substances 0.000 description 3
- 229940088597 hormone Drugs 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241001468611 Polygonatum cyrtonema Species 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000004043 dyeing Methods 0.000 description 2
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 238000009331 sowing Methods 0.000 description 2
- 102000018997 Growth Hormone Human genes 0.000 description 1
- 108010051696 Growth Hormone Proteins 0.000 description 1
- 208000012868 Overgrowth Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004099 anaerobic respiration Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 230000019371 dormancy process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000122 growth hormone Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 229930002348 tetracyclic diterpenoid Natural products 0.000 description 1
- -1 tetracyclic diterpenoid compounds Chemical class 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
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- Agronomy & Crop Science (AREA)
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- Toxicology (AREA)
- Botany (AREA)
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- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for promoting germination of polygonatum sibiricum seeds, which comprises the following steps: (1) Soaking rhizoma Polygonati seeds in gibberellin solution for a set time, transferring to a substrate for culturing, and removing dormancy for one time to obtain nascent rhizome; (2) And (3) soaking the primary rhizome in a growth regulator solution for a set time, refrigerating the primary rhizome, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the sealwort seedling. The method for promoting seed germination has a good effect on breaking the dormancy of the polygonatum sibiricum seeds, the incidence rate of primary rhizomes of the seeds and the emergence rate of the primary rhizomes are remarkably improved, the success rate of breaking the primary dormancy is over 80 percent, and the success rate of breaking the secondary dormancy is over 90 percent; meanwhile, the seedling emergence time of the polygonatum sibiricum seeds is greatly shortened, and the time cost of breeding and seedling raising of the polygonatum sibiricum is reduced.
Description
Technical Field
The invention relates to the technical field of crop breeding and seedling raising, in particular to a method for promoting germination of polygonatum sibiricum seeds.
Background
The dormancy of the sealwort seeds belongs to comprehensive dormancy. The structure of the seed coat and the endosperm makes the seeds absorb water slowly; after the seeds are mature, a physiological dormancy process is carried out to make the seeds enter a dormant state; the presence of germination inhibiting substances in the seeds affects the germination of the seeds. Water absorption and dissection experiments show that the structures of the seed coat and the endosperm limit the penetration of water; huang Jingpei has few contents, is not physiologically mature, does not meet the requirements of germination, and requires a long time to provide a material basis for germination. Fermentation, temperature change and stratification are adopted to break dormancy and promote the after-ripening process of embryos. The existing breeding process of polygonatum generally adopts the following method: collecting mature polygonatum cyrtonema fruits at the bottom of 10 months in the current year, rubbing and washing to obtain clean seeds, storing the seeds in sand for 1 winter, taking out the seeds in the middle 3 months of the second year, sowing the seeds in a flowerpot with a moist and fertile substrate, and germinating in spring of the third year; the whole process from sowing seeds to seedling growth generally needs 12 months, and the period is long. The prior document discloses a technical scheme for promoting germination of polygonatum sibiricum seeds by soaking seeds in clear water within the temperature range of 25-50 ℃ (Zhu Wufeng, medicinal plant polygonatum sibiricum breeding technical research [ D ], northwest agriculture and forestry science and technology university, 2013), although the effect of shortening the breeding seedling time to a certain extent can be produced, the technical problem of low emergence rate still exists in the practical application process.
Disclosure of Invention
The invention provides a method for promoting germination of polygonatum sibiricum seeds, which is used for solving the technical problems of overlong emergence period and low emergence rate of the existing polygonatum sibiricum breeding and seedling raising technology.
In order to solve the technical problem, the invention adopts the following technical scheme:
a method for promoting germination of polygonatum sibiricum seeds comprises the following steps:
(1) Soaking the polygonatum sibiricum seeds in gibberellin solution for a set time, transferring the polygonatum sibiricum seeds to a substrate for culture, and removing the dormancy for the first time to obtain primary rhizomes;
(2) And (3) soaking the primary rhizome in a growth regulator solution for a set time, refrigerating the primary rhizome, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the polygonatum sibiricum seedling.
The design idea of the technical scheme is that gibberellin is an important plant growth hormone, belongs to tetracyclic diterpenoid compounds, and the study of breaking seed dormancy through gibberellin treatment is carried out in the prior art, but the inventor finds that the germination of polygonatum cyrtonema seeds is more obviously influenced by gibberellin compared with other plants.
The hormone soaking concentration, the treatment temperature and the treatment time have great significance for promoting seed germination, low-concentration hormone can hardly activate related signal paths, and high-concentration hormone can inhibit related growth and development processes; proper soaking temperature can create a proper environment for starting germination of seeds, the activity of the seed enzyme is not high at low temperature, and related enzymes of the seeds are inactivated at high temperature, so that the seeds cannot germinate; the proper soaking time is also the same, the soaking time is too short to achieve the effect, and the soaking time is too long, so that the seeds can be suffocated or anaerobic respiration can be started, the seeds are poisoned, and the germination rate is reduced.
As a further preferred aspect of the above technical means, the gibberellin solution is GA 1 The solution of (1). Gibberellins are widely available, and there are a total of 136 gibberellins that have been discovered, collectively referred to as Gibberellins (GA) S ) The prior art generally adopts GA 3 The seeds were treated to promote germination, but the inventors found that GA was present during the actual cultivation 3 Because the activity is too high, the higher mildew rate of the seeds is caused, the germination rate of the seeds is influenced, the overgrowth of the embryonic axis is promoted when the dormancy of the plants is broken, and the lodging resistance of the plants is reduced; based on this study, the inventors found that GA 1 The method is more suitable for treating the seeds of the polygonatum sibiricum, can smoothly break the dormancy of the seeds, does not cause the growth of an embryonic axis, avoids the lodging phenomenon of plants, and simultaneously reduces the mildewing rate of the seeds, thereby improving the germination rate of the seeds.
As a further preferred mode of the above technical means, the concentration of the gibberellin solution is 30 to 50mg/L. The concentration of the gibberellin solution is one of the key parameters influencing the incidence rate of the primary rhizomes of the seeds, and the polygonatum sibiricum seeds have the optimal incidence rate of the primary rhizomes after being treated by the gibberellin solution within the concentration range.
As a further optimization of the technical scheme, in the step (1), the sealwort seeds are soaked in the gibberellin solution for 24-72 hours at the temperature of 30-40 ℃. After the soaking treatment within the time and temperature ranges, the polygonatum sibiricum seeds have the optimal incidence rate of primary rhizomes.
In a more preferred embodiment of the above aspect, the growth regulator solution is a 6- (2-hydroxybenzylamino) purine solution.
In a more preferable embodiment of the above aspect, the concentration of the growth regulator solution is 200 to 400mg/L.
As a further optimization of the technical scheme, the soaking time of the sealwort seeds in the growth regulator solution in the step (2) is 12-24 h.
As a further preferred mode of the above technical means, the cold storage temperature of the nascent rhizome in the step (2) is 4 ℃ and the cold storage time is 30 to 60 days.
Preferably, the polygonatum seeds are obtained by picking immature polygonatum fruits, removing impurities, spreading and drying for two days to volatilize part of water, sealing at 4 ℃, fermenting at low temperature for 90 days, taking out, rubbing and washing seed coats in clear water, and collecting seeds which are submerged under water.
Compared with the prior art, the invention has the advantages that:
the method for promoting seed germination has a good effect on breaking the dormancy of the polygonatum sibiricum seeds, the incidence rate of the primary rhizomes of the seeds and the emergence rate of the primary rhizomes are remarkably improved, the success rate of breaking the primary dormancy is over 80 percent, and the success rate of breaking the secondary dormancy is over 90 percent; meanwhile, the seedling emergence time of the polygonatum sibiricum seeds is greatly shortened, and the time cost of breeding and seedling raising of the polygonatum sibiricum is reduced.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1:
the method for promoting germination of polygonatum sibiricum seeds comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the vitality of the sealwort seeds comprises two steps, and firstly, the vitality of the seeds is tested by adopting a TTC method: soaking seeds in water bath at 30 ℃ for 6h, and dyeing for 24h in a 0.3% TTC solution in dark. Placing in a culture dish, cutting seeds to expose obvious embryo structure as much as possible, and simultaneously boiling the seeds with boiling water for comparison treatment, wherein the red dyed embryos are live seeds; and then testing the seed vitality by adopting a red ink method: and (3) taking the remaining half of the seeds obtained by the TTC method, placing the seeds in a culture dish, adding 5% red ink for dyeing for 20 minutes, pouring out the red ink, then washing the seeds for multiple times by using tap water, and observing the coloring condition. The seed embryo which is not colored or is slightly colored is a live seed, and the seed embryo which is the same as the endosperm in coloring degree is a dead seed. Taking the average value of the results of the two detection methods as the final determination result of the seed activity;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 1 Soaking in the solution for 72h, transferring to matrix for culture, and releasing dormancy to obtainSoaking the primary rhizome at 40 deg.C; tests show that the incidence rate of primary rhizomes of sealwort seeds treated in the step (2) is 77.3 percent, and the mildew rate of the seeds is 5 percent;
(3) Soaking the primary rhizome in 400 mg/L6- (2-hydroxybenzylamino) purine solution for 24h, refrigerating the primary rhizome at 4 deg.C for 60 days, transferring to substrate for cultivation, and removing secondary dormancy to obtain rhizoma Polygonati seedling; the germination rate of the nascent rhizomes treated by the step (3) is 90% through tests.
Example 2:
the method for promoting germination of polygonatum sibiricum seeds comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 40mg/L 1 Soaking in the solution for 24h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 30 deg.C; tests show that the incidence rate of the primary rhizomes of the sealwort seeds treated in the step (2) is 68 percent, and the mildew rate of the seeds is 8.7 percent;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 60 days, transferring to substrate for cultivation, and removing secondary dormancy to obtain rhizoma Polygonati seedling; the germination rate of the primary rhizomes treated by the step (3) is 81.9 percent through tests.
Example 3:
the method for promoting germination of polygonatum sibiricum seeds comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the activity determination steps of the sealwort seeds are the same as the example 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 30mg/L 1 Soaking in the solution for 24h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 30 deg.C; tests show that the incidence rate of the primary rhizomes of the sealwort seeds treated in the step (2) is 67.3 percent, and the mildew rate of the seeds is 9.3 percent;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 60 days, transferring to substrate for cultivation, and removing secondary dormancy to obtain rhizoma Polygonati seedling; according to the test, the germination rate of the primary rhizome treated by the step (3) is 82.3%.
Example 4:
the method for promoting germination of polygonatum sibiricum seeds comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 1 Soaking in the solution for 48h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 35 deg.C; tests show that the incidence rate of the primary rhizomes of the sealwort seeds treated in the step (2) is 72.7 percent, and the mildew rate of the seeds is 7.3 percent;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 60 days, transferring to substrate for cultivation, and breaking secondary dormancy to obtain rhizoma Polygonati seedling; the test shows that the germination rate of the nascent rhizomes treated by the step (3) is 80.3 percent.
Example 5:
the method for promoting germination of polygonatum sibiricum seeds comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 1 Soaking in the solution for 72h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 40 deg.C; tests show that the incidence rate of the primary rhizomes of the sealwort seeds treated in the step (2) is as high as the mildew rate of the seeds;
(3) Soaking the primary rhizome in 200 mg/L6- (2-hydroxybenzylamino) purine solution for 24h, refrigerating the primary rhizome at 4 deg.C for 60 days, transferring to substrate for cultivation, and removing secondary dormancy to obtain rhizoma Polygonati seedling; the germination rate of the primary rhizomes treated by the step (3) is 63% through tests.
Example 6:
the method for promoting germination of polygonatum sibiricum seeds comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 1 Soaking in the solution for 72h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 40 deg.C; tests show that the incidence rate of the primary rhizomes of the sealwort seeds treated in the step (2) is as high as the mildew rate of the seeds;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma Polygonati seedlings, wherein the germination rate of the primary rhizome treated in step (3) is 83% by test.
Comparative example 1:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 3 Soaking in the solution for 48h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 40 deg.C;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 30 days, transferring to substrate, and removing secondary dormancy to obtain rhizoma Polygonati seedling.
The conditions of the primary rhizomes of the sealwort seeds treated in the step (2) are tested, and the incidence rate of the primary rhizomes of the sealwort seeds treated by the comparative example is only 10%, and the mildewing rate of the seeds is as high as 22.5%.
In the comparative example, the soaking temperature in the step (2) is adjusted to 23 ℃, 26 ℃, 29 ℃ and 32 ℃, other parameters are unchanged, and the conditions of the primary rhizomes of the polygonatum sibiricum seeds treated in the step (2) are tested, and the results are shown in the following table 1:
TABLE 1 gibberellin GA at different soaking temperatures 3 Influence on primary dormancy removal of rhizoma Polygonati seeds
| Temperature of culture | Percentage of germination | Rate of mildew formation |
| 23℃ | 3％ | 17.5％ |
| 26℃ | 10％ | 22.5％ |
| 29℃ | 0 | 32.5％ |
| 32℃ | 0 | 66.7％ |
This comparative example also adjusted gibberellin GA in step (2) 3 The concentration of (3) is set to be 0mg/L, 10mg/L and 30mg/L, other parameters are not changed, and the condition of the primary rhizome of the sealwort seed treated in the step (2) is tested, and the results are shown in the following table 2:
TABLE 2 gibberellin GA at different concentrations 3 Influence on primary dormancy breaking of rhizoma Polygonati seeds
| 0mg/L | 10mg/L | 30mg/L | 50mg/L | |
| GA 3 | 10.20％ | 30％ | 21.70％ | 24％ |
The above test results show that GA 3 Seed ratio GA for inducing germination 1 The induced germination seeds have high mildewing rate and low later-stage survival rate. And the embryo axis is too long, so that the nutrition of the embryo is consumed, and the development of seedling emergence at the later stage is not facilitated.
Comparative example 2:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 4+7 Soaking in the solution for 48h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 40 deg.C;
(3) Soaking the primary rhizome in KT solution with the concentration of 300mg/L for 12h, refrigerating the primary rhizome at the refrigerating temperature of 4 ℃ for 30 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the sealwort seedling.
The secondary dormancy breaking condition of the primary rhizome treated in the step (3) of the comparative example was tested, and the result showed that the germination percentage of the primary rhizome after the secondary dormancy breaking was 53.33%.
Comparative example 3:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Respectively subjecting rhizoma Polygonati seeds to gibberellin GA with concentration of 10mg/L and 20mg/L 4+7 Soaking in the solution for 72h, transferring to substrate for culture, removing dormancy to obtain primary rhizome, and soaking at 40 deg.C;
(3) Soaking the primary rhizome in 400 mg/L6- (2-hydroxybenzylamino) purine solution for 24h, refrigerating the primary rhizome at 4 deg.C for 60 days, transferring to substrate, and removing secondary dormancy to obtain rhizoma Polygonati seedling.
The primary rhizome conditions of the sealwort seeds treated in the step (2) are tested, and the results show that the incidence rates of the primary rhizome of the sealwort seeds treated in the step (2) are respectively 61.90% (gibberellin concentration is 10 mg/L) and 67.70% (gibberellin concentration is 20 mg/L).
Comparative example 4:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Concentrating rhizoma Polygonati seedGibberellin GA with a degree of 50mg/L 4+7 Soaking in the solution for 48h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 40 deg.C;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 30 days, transferring to substrate, and removing secondary dormancy to obtain rhizoma Polygonati seedling.
The secondary dormancy breaking condition of the primary rhizomes treated in the step (3) of the comparative example was tested, and the results showed that the germination percentage of the primary rhizomes of the comparative example after the secondary dormancy breaking was only 33.33%.
Comparative example 5:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 4+7 Soaking in the solution for 48h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 26 deg.C;
(3) Soaking the primary rhizome in a 2,4D solution with the concentration of 300mg/L for 12h, refrigerating the primary rhizome at 4 ℃ for 30 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the sealwort seedling.
The secondary dormancy breaking condition of the primary rhizome treated in the step (3) of the comparative example was tested, and the result showed that the germination percentage of the primary rhizome of the comparative example after the secondary dormancy breaking was only 6.67%.
Comparative example 6:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 4+7 Soaking in the solution for 48h, transferring to substrate, culturing, and releasing dormancy to obtain primary rhizome, soaking at 20 deg.C and 25 deg.C respectively;
(3) Soaking the primary rhizome in 400 mg/L6- (2-hydroxybenzylamino) purine solution for 24h, refrigerating the primary rhizome at 4 deg.C for 60 days, transferring to substrate, and removing secondary dormancy to obtain rhizoma Polygonati seedling.
Comparative example 7:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the method for determining the activity of the sealwort seeds is the same as the method in the embodiment 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 1 Soaking in the solution for 48h, transferring to substrate for culture, removing dormancy to obtain primary rhizome, and soaking at 40 deg.C;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 30 days, transferring to substrate, and removing secondary dormancy to obtain rhizoma Polygonati seedling.
The conditions of the primary rhizomes of the sealwort seeds treated in the step (2) are tested, and the incidence rate of the primary rhizomes of the sealwort seeds treated by the comparative example is 74%, and the mildewing rate of the seeds is 8%.
Comparative example 8:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the activity determination steps of the sealwort seeds are the same as the example 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 4 Soaking in the solution for 48h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 40 deg.C;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 30 days, transferring to substrate, and removing secondary dormancy to obtain rhizoma Polygonati seedling.
The conditions of the primary rhizomes of the sealwort seeds treated in the step (2) are tested, and the incidence rate of the primary rhizomes of the sealwort seeds treated by the comparative example is 16%, and the mildewing rate of the seeds is 18.7%.
Comparative example 9:
the method for promoting germination of polygonatum sibiricum seeds in the comparative example comprises the following steps:
(1) Picking incompletely ripe rhizoma Polygonati fruits from 8-month-first ten days to middle ten days, removing impurities, spreading and drying for two days to volatilize partial water, subpackaging and sealing at 4 ℃ by using a self-sealing bag, fermenting at low temperature 90d, taking out at the bottom of 10 months, putting in clear water to scrub seed coats, collecting seeds submerged under water, and measuring the activity of the seeds; the activity determination steps of the sealwort seeds are the same as the example 1;
(2) Subjecting rhizoma Polygonati seed to gibberellin GA with concentration of 50mg/L 7 Soaking in the solution for 48h, transferring to substrate for culture, and removing dormancy to obtain primary rhizome, soaking at 40 deg.C;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4 deg.C for 30 days, transferring to substrate, and removing secondary dormancy to obtain rhizoma Polygonati seedling.
The conditions of the primary rhizomes of the sealwort seeds treated in the step (2) are tested, and the incidence rate of the primary rhizomes of the sealwort seeds treated by the comparative example is 18.7%, and the mildewing rate of the seeds is 14.7%.
The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above-described embodiments. Modifications and variations that may occur to those skilled in the art without departing from the spirit and scope of the invention are to be considered as within the scope of the invention.
Claims (9)
1. A method for promoting germination of polygonatum sibiricum seeds is characterized by comprising the following steps:
(1) Soaking the sealwort seeds in gibberellin solution for a set time, transferring the sealwort seeds to a substrate for culture, and removing the dormancy for one time to obtain primary rhizomes;
(2) And (3) soaking the primary rhizome in a growth regulator solution for a set time, refrigerating the primary rhizome, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the sealwort seedling.
2. The method for promoting germination of polygonatum sibiricum seeds according to claim 1, wherein the gibberellin solution is GA 1 The solution of (1).
3. The method for promoting germination of polygonatum sibiricum seeds according to claim 1, wherein the concentration of the gibberellin solution is 30-50 mg/L.
4. The method for promoting germination of polygonatum sibiricum seeds according to claim 1, wherein the polygonatum sibiricum seeds in the step (1) are soaked in the gibberellin solution for 24-72 hours at a temperature of 30-40 ℃.
5. The method for promoting germination of polygonatum sibiricum seeds according to claim 1, wherein the growth regulator solution is 6- (2-hydroxybenzylamino) purine solution.
6. The method for promoting germination of polygonatum sibiricum seeds according to claim 5, wherein the concentration of the growth regulator solution is 300-400 mg/L.
7. The method for promoting germination of polygonatum sibiricum seeds according to claim 6, wherein the soaking time of the polygonatum sibiricum seeds in the growth regulator solution in the step (2) is 12-24 hours.
8. The method for promoting germination of polygonatum sibiricum seeds according to claim 6, wherein the cold storage temperature of the nascent rhizome in the step (2) is 4 ℃ and the cold storage time is 30-60 days.
9. The method for promoting germination of polygonatum sibiricum seeds according to any one of claims 1 to 8, wherein the polygonatum sibiricum seeds are obtained by picking up immature polygonatum sibiricum fruits, removing impurities, spreading and drying for two days to volatilize part of water, sealing at 4 ℃, taking out after fermenting at low temperature for 90 days, putting in clear water to scrub seed coats, and collecting seeds settled under water.
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| CN116235762A (en) * | 2023-05-12 | 2023-06-09 | 江西农业大学 | Method for rapidly raising seedlings of polygonatum cyrtonema seeds |
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| CN105660219A (en) * | 2016-01-20 | 2016-06-15 | 贵州大学 | Polygonatum cyrtonema seed rapid emerging method |
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| CN105660219A (en) * | 2016-01-20 | 2016-06-15 | 贵州大学 | Polygonatum cyrtonema seed rapid emerging method |
| CN109463064A (en) * | 2018-12-17 | 2019-03-15 | 安徽农业大学 | A kind of preprocess method promoting polygonatum cyrtonema seed seedling |
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