CN107529398B - A kind of detoxicated ginger mating system - Google Patents
A kind of detoxicated ginger mating system Download PDFInfo
- Publication number
- CN107529398B CN107529398B CN201711001036.1A CN201711001036A CN107529398B CN 107529398 B CN107529398 B CN 107529398B CN 201711001036 A CN201711001036 A CN 201711001036A CN 107529398 B CN107529398 B CN 107529398B
- Authority
- CN
- China
- Prior art keywords
- parts
- ginger
- seedling
- detoxicated
- detoxicated ginger
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a kind of detoxicated ginger mating systems, comprising the following steps: (1) preparation of detoxicated ginger seedling；(2) expanding propagation of detoxicated ginger seedling；(3) transplanting domestication: by step (2) obtain a large amount of detoxicated ginger seedlings plant in cultivation matrix, in 20-28 DEG C culture 1-2 weeks；Wherein, cultivation matrix is the mixture that perlite, peat soil and bio-bacterial manure are 1-2:3-5:0.3-0.8 mixing by volume；Nitrogen, phosphorus and potassium concn are respectively 60-100mg/L, 20-40mg/L and 100-150mg/L in culture early period, and later period nitrogen, phosphorus and potassium concn are respectively 200-220mg/L, 20-40mg/L and 200-250mg/L, and fertilising is primary weekly.The present invention studies each parameter of entire reproductive process, and institute's Optimal Parameters, which combine, can effectively improve detoxicated ginger rate, survival rate, yield, shortens its breeding cycle.
Description
Technical field
The invention belongs to ginger raising technology fields, and in particular to a kind of detoxicated ginger mating system.
Background technique
Ginger (Zingiber officinale Roscoe) is herbaceos perennial, has treatment diarrhea, appetizing strong
Spleen refreshes oneself, anti-aging, treats internal disease, health and other effects.It, which is bred, mainly carries out vegetative propagation by underground rhizomes,
Since its growth and development is without sexual generation, virus easily accumulates in vivo, leads to ginger leaves shrinkage, slow growth, yield drop
The problems such as low and quality deterioration.Therefore, the virus for sloughing ginger keeps the kind of excellent variety very must modern ginger cultivation
It wants.But now bad to ginger progress detoxification efficiency, the breeding of detoxic seedling also fails to really realize as toxicity-removing white potato, strawberry etc.
Industrialization, main reason is that ginger is 1 year single batch of long-term cropping, the period of breeding kind ginger is longer, and is not yet formed at present
A set of cost-effective rhizome mating system.
Summary of the invention
For above-mentioned deficiency in the prior art, the present invention provides a kind of detoxicated ginger mating systems, can effectively solve
Detoxicated ginger is ineffective in the prior art, the problem long at the mature ginger original seed period of the tissue-cultured seedling breeding after detoxification.
To achieve the above object, the technical solution adopted by the present invention to solve the technical problems is:
A kind of detoxicated ginger mating system, comprising the following steps:
(1) preparation of detoxicated ginger seedling
The young shoot for taking ginger rhizomatic germination peels off outer layer leaf sheath, clean with aseptic water washing, then with 75% alcohol disinfecting
30-40s, with aseptic water washing 3-4 times, then with 0.1% HgCl28-10min is sterilized, continues to use aseptic water washing 5-6 times,
Young shoot is removed under anatomical lens, the access of 0.2-0.5cm shoot tip meristem is taken to contain 6-BA 0.5-1mg/L, KT 0.5-
In the MS culture medium of 1.5mg/L, NAA 0.05-0.15mg/L and sucrose 15-20g/L, it is placed in 56-60 DEG C of heat treatment 40-
Then 60min is cultivated, first under dark condition, 23-26 DEG C culture 2-3 days, then be placed in culturing room and normally cultivate 28-30
Its (23 ± 1 DEG C of culturing room's day temperature, 18 ± 1 DEG C of nocturnal temperature, illumination 2000-3000lx, light application time 14h/d) is obtained
Tissue-cultured seedling is regenerated, clip regenerates tissue-cultured seedling cauline leaf and carries out viral diagnosis, after dividing to not viruliferous tissue-cultured seedling base portion by simple bud,
Again it is transferred in new culture medium (identical as front culture medium) and carries out squamous subculture, obtain detoxicated ginger seedling；
(2) expanding propagation of detoxicated ginger seedling
By step (1) obtain detoxicated ginger seedling cut off cauline leaf after, to its base portion by simple bud divide, renewed vaccination to newly
MS culture medium containing 6-BA 0.5-1mg/L, KT 0.5-1.5mg/L and NAA 0.05-0.15mg/L and sucrose 15-20g/L
Middle carry out Multiplying culture is cultivated 40-45 days, and largely band root detoxicated ginger seedling is obtained；
(3) transplanting domestication
It survives culture: a large amount of detoxicated ginger seedlings that step (2) obtain being planted in cultivation matrix, 20-28 DEG C of culture is placed in
1-2 weeks；Wherein, it is the mixed of 1-2:3-5:0.3-0.8 mixing that cultivation matrix is perlite, peat soil and bio-bacterial manure by volume
Close object；
Culture early period: applying fertilizer to the detoxic seedling after surviving, every 2 plants of fertilisings 150-170mL, the concentration of nitrogen, phosphorus and potassium
Respectively 60-100mg/L, 20-40mg/L and 100-150mg/L, fertilising is primary weekly；
Later period culture: after detoxic seedling is grown 13-14 weeks, every 2 plants of fertilisings 150-170mL, the concentration of nitrogen, phosphorus and potassium is respectively
200-220mg/L, 20-40mg/L and 200-250mg/L, fertilising is primary weekly；
Leaf surface trace fertilizer (containing various trace elements) while monthly is monthly sprayed in culture early period and later period incubation
Carry out an insect pest preventing and controlling.
Further, used medium is to contain 6-BA 0.5mg/L, KT 1.2mg/L in step (1) neutralization procedure (2)
With the MS culture medium of NAA 0.05mg/L and sucrose 16g/L.
Further, heat treatment process is 60 DEG C of heat treatment 40min in step (1).
Further, bio-bacterial manure comprises the following components in parts by weight: 1-3 parts of compound bacteria, 30-40 parts of corn flour, wheat bran
10-20 parts, 2-4 parts of sucrose, 20-30 parts of fulvic acid, 5-10 parts of ginkgo leaf, 0.1-0.3 parts of ammonium molybdate, zinc sulfate 0.05-
0.08 part and water 8-15 parts.
Further, bio-bacterial manure comprises the following components in parts by weight: 2 parts of compound bacteria, 40 parts of corn flour, 15 parts of wheat bran,
3 parts of sucrose, 28 parts of fulvic acid, 6 parts of ginkgo leaf, 0.2 part of ammonium molybdate, 0.06 part of zinc sulfate and 12 parts of water.
Further, compound bacteria comprises the following components in parts by weight: 1-2 parts of nitrogen-fixing bacteria, 1-2 parts of phosphate solubilizing bacteria, potassium solubilizing bacteria 1-2
Part, 2-4 parts of bacillus subtilis.
Further, compound bacteria comprises the following components in parts by weight: 1.2 parts of nitrogen-fixing bacteria, 1 part of phosphate solubilizing bacteria, potassium solubilizing bacteria 1.5
Part, 3 parts of bacillus subtilis.
Further, bio-bacterial manure is prepared by the following method to obtain:
Ginkgo leaf is crushed, is then mixed with corn flour, wheat bran and suitable quantity of water, compound bacteria is then added, stirs evenly, so
After add fulvic acid and excess water, mix, be subsequently placed in 30-35 DEG C of fermentation 15-20 days, it is obtained.
Further, every 2 plants of fertilisings 150mL, the concentration of nitrogen, phosphorus and potassium are respectively in step (3) incubation early period
60mg/L, 20mg/L and 100mg/L.
Further, every 2 plants of fertilisings 150mL, the concentration of nitrogen, phosphorus and potassium are respectively in step (3) later period incubation
200mg/L, 20mg/L and 200mg/L.
Detoxicated ginger mating system provided by the invention, has the advantages that
(1) present invention obtains aseptic seedling using stem tip tissue culture, then removes to aseptic seedling, different by being arranged
The indexs such as heat treatment temperature, time, stem apex length, filter out preferably heat treatment mode, at that time 60 DEG C of heat treatment 40min, stem
Sharp length is 0.2-0.5cm, and the detoxic seedling death rate cultivated is low, proliferation rate and virus elimination rate are higher.
(2) during transplanting domestication, cultivation matrix used contains perlite, peat soil and bio-bacterial manure.
Perlite, apparent density is light, thermal coefficient is low, chemical stability is good, wettability power is small, and nontoxic, tasteless, fire prevention
Etc. functions required air can be provided for detoxicated ginger seedling in the present invention as a part of cultivation matrix, can be with
Moisture-keeping function is played, many nutriments are also contained, nutrition can be provided for the growth of detoxicated ginger seedling.
Peat soil can provide a large amount of nutriment containing a large amount of organic substrate for detoxicated ginger seedling, meanwhile, it
Also there is the advantage of water conservation and good permeability to cooperate in conjunction with perlite and provide good growing environment for detoxicated ginger seedling, mention
For required nutriment, water conservation and gas permeability grow detoxicated ginger seedling quickly.
Bio-bacterial manure is grouped as by specific group, containing multiple beneficial bacterium, can improve the fertility of cultivation matrix, also containing more
Kind microelement and nutriment are prepared rationally, enhance strengthening root system, and absorption of the detoxicated ginger seedling to nutriment is increased, from
And achieve the purpose that beneficial root, strong sprout；Meanwhile also containing ginkgo leaf in bio-bacterial manure, with bacteriostasis, with other substance knots
It closes after everfermentation, nutriment can be provided for detoxicated ginger seedling, promote its growth, meanwhile, also accomplish waste utilization, has dropped
Its low production cost.
(3) domestication it is each during, based on cultivation matrix, the present invention also applied npk fertilizer, each
The content in stage, N P and K used is different, and cultivation matrix combines fertilizer used, can effectively facilitate the life of blade and underground rhizome
It is long, so as to shorten its growth cycle, reduce manufacturing cost.
(4) present invention studies each parameter of entire reproductive process, optimizes each parameter and combines and can effectively mention
High detoxicated ginger rate, survival rate, yield, shorten its breeding cycle.
Specific embodiment
The preparation of 1 detoxicated ginger seedling of embodiment
1, influence of the different heat treatment to ginger aseptic seedling survival rate, proliferation rate and virus elimination rate
The young shoot for taking ginger rhizomatic germination peels off outer layer leaf sheath, clean with aseptic water washing, then with 75% alcohol disinfecting
30-40s, with aseptic water washing 3-4 times, then with 0.1% HgCl28-10min is sterilized, continues to use aseptic water washing 5-6 times,
Young shoot is removed under anatomical lens, take different length shoot tip meristem access containing 6-BA 0.5mg/L, KT 1.2mg/L,
In the MS culture medium of NAA 0.05mg/L and sucrose 16g/L, heat treatment temperature (A), processing time (B) and stem apex length (C) three
A factor, totally 9 processing, each processing are inoculated with 8 bottles, and every bottle connects 4 stem apexs, are then cultivated, first under dark condition, 25
It DEG C culture 2 days, then is placed in culturing room and normally cultivates (23 ± 1 DEG C of culturing room's day temperature, 18 ± 1 DEG C of nocturnal temperature, illumination in 30 days
2000-3000lx, light application time 14h/d), regeneration tissue-cultured seedling is obtained, clip regenerates tissue-cultured seedling cauline leaf and carries out viral diagnosis, to not
After viruliferous tissue-cultured seedling base portion is by simple bud segmentation, it is transferred in new culture medium (identical as front culture medium) again and carries out subculture
Culture, obtains detoxicated ginger seedling.Observation and statistical data respectively after 1 week and January, calculate the death rate and proliferation rate, the death rate=dead
Strain number/inoculation number × 100% of the plant died；Total seedling number/inoculation number × 100%, the results are shown in Table 1, table 2 after proliferation rate=inoculation
With table 3.
Wherein, the viral diagnosis of detoxic seedling is as follows:
(1) it detects the preparation of liquid: taking the aseptic seedling plant after cultivating, be placed on superclean bench, every processing randomly selects 3
Strain (3-5cm), which is put into 3 mortars for bacterium of having gone out in advance, to be fully ground, and distilled water is added to extract juice, pours into sterilized
In centrifuge tube, after 2000r/min is centrifuged 5min, stand for standby use is compared with untreated aseptic seedling.
(2) painting of 100 μ l supernatants the screening of Pseudomonas solanacearum: is drawn from the centrifuge tube of each alignment processing respectively
Cloth does 4 blank plates and compares as environmental pollution on peptone culture medium plate.Plate is placed in 28 DEG C -30 DEG C
It is cultivated 1 day in incubator, during which pays attention to observing, after growing bacterium colony, different single colonies isolated with streaking inoculation, is first existed
Its morphological feature of optical microphotograph microscopic observation.Different single colonies is marked, then the different single colonie film-making of picking →
The crystal violet liquid dyeing 1min → ethanol decolorization of iodine staining 1min → 95% processing 20s → sarranine redyes 1min → drying → mirror
Inspection, wherein will be washed after dyeing and decoloration.
(3) detection of Pseudomonas solanacearum: choosing the label bacterium colony that takes on a red color after dyeing, sprinkling 0.005%TTC in
On bacterium colony, colony colour is observed after 30min, counts each processing and its number of plates of purple bacterium colony is presented in control, and calculate each
The virus elimination rate of a processing and its control, the total plant number of plant number/detection of virus elimination rate=detect Pseudomonas solanacearum
× 100%.
1 ginger of table is heat-treated detoxification L9(34) orthogonal test
Note: CK be heat treatment control, "-" indicate without.
2 ginger of table is heat-treated detoxification L9(34) orthogonal experiments intuitive analysis
Note: K be factor and, X is average value, and R is very poor.
3 ginger of table is heat-treated detoxification L9(34) orthogonal experiments variance analysis
Note: F indicates variance, and P indicates significant difference.
As shown in Table 1, the ginger energy normal growth after different high-temperature process, forms plant.Wherein, processing 1,2,3,
4,5,7,9 survival rates are 100.00%, and 6 death rates of processing are up to 30.00%, and processing 8 is taken second place, the death rate 3.33%.
From the point of view of proliferation rate, highest aseptic seedling proliferation rate after Overheating Treatment is processing 3 (proliferation rate 256.67%), growth coefficient
Compared with CK (heat treatment control) mostly 0.1 multiplying factor (246.67%)；It followed by handles 4 > and handles 5 > processing 7, worst is processing
6, proliferation rate is only 70.00%.For virus elimination rate, 9 aseptic seedling stem apex+heat treatment mode detoxification efficiency difference are very big,
Average virus elimination rate 51.85%.Wherein processing 6 and 8 virus elimination rates of processing followed by handle 1 up to 100.00%, and virus elimination rate is
66.67%, processing 5 and CK detoxification efficiency is worst, virus elimination rate 0.00%.The plant lapping liquid of pathogen could not be removed in albumen
The Pseudomonas solanacearum detected on peptone agar plate has following characteristics: bacterium colony is in subcircular, protuberance, relatively moistens, is smooth,
Fester has stink in faint yellow；Its bacterial strain is in the shape of a rod under low-powered microscope；It is presented through Gram's staining red；Sprinkling
After 0.005%TTC, purple is presented in Pseudomonas solanacearum bacterium colony.
Intuitive analysis (table 2) finds out that RC > RA=RB, the primary-slave relation for influencing the ginger death rate is at stem apex length > heat
Manage temperature=heat treatment time.The blank factor is used to carry out variance analysis (table 3) to tri- factors of A, B, C as error term, as a result
For FC (1.37) > FA (1.00)=FB (1.00), factor B is identical with factor A variance, and the significant difference P of three is all larger than
0.05, illustrate that difference is not significant.It is maximum to can be seen that factor C influences the death rate of ginger by the comparison of F value, this divides with intuitive
Analysis result is consistent, shows that the stem apex length of aseptic seedling influences most its death rate by intuitively analyzing result and the results of analysis of variance
Greatly.Comprehensively consider the survival rate of aseptic seedling, worst to handle 6 (A2B3C1), followed by 8 (A3B2C1) of combination.In addition, in ginger
It is found in the routine observation of seedling growing state, the more untreated seedling of the seedling after Overheating Treatment (control) growing way is slow, illustrates heat
Processing is survived to it and growth has inhibiting effect.For the proliferation rate of Jiang Miao (table 3), the factor primary and secondary of ginger seedling proliferation rate is influenced
Relationship is C > A > B, carries out variance analysis to A, B, C using the blank factor as error term.The results show that determining theirs by F value
Primary-slave relation is C > B > A, and factor C (stem apex length) influences the proliferation rate of ginger maximum.By PA (0.7111) > PB
(0.5368) > PC (0.0790) is as can be seen that 3 factors are not up to the level of signifiance to the influence degree of proliferation rate.Consider ginger
The proliferation rate of seedling, test optimum combination are 3 (A1B3C3D3) of processing, this is consistent with main factor and intuitive analysis result is considered.It says
It is bright to remove aseptic seedling, take the stem apex of 1.0cm or so to be seeded on culture medium, 52 DEG C of processing 60min are optimal processing methods.
From the point of view of table 2, the primary-slave relation of the factor of Jiang Miao virus elimination rate is influenced are as follows: C > A=B, the blank factor is as error term to A, B, C
Variance analysis is done, from table 3 it can be seen that FC > FA=FB.The factor relation for influencing its detoxification efficiency that F value determines is C > A=
The principal element that B, i.e. stem apex length are ginger aseptic seedling detoxification efficiency.And 0.01 < PC (0.0345) <, 0.05 < PA
(0.1999)=PB (0.1999), C factor (aseptic seedling stem apex length) have reached the level of signifiance, A factor (heat treatment temperature) and B
Factor does not reach the level of signifiance.Stem apex length is in 0.6-0.9cm and 1.0- to be shown to the comparison between 3 levels of C factor
Difference is not significant in 1.3cm level, and 0.2-0.5cm and 0.6-0.9cm, 0.2-0.5cm and 1.0-1.3cm level difference are significant,
Illustrate that there were significant differences for virus elimination rate of the different stem apex length to ginger.As a result, to handle 8 (A3B2C1) of 6 (A2B3C1) and processing
Optimal, because the proliferation rate (113.33%) of processing 8 is higher than the proliferation rate of 6 (70.00%) of processing, combination 8 is better than combination
6, processing 8 is optimum combination.Orthogonal arrage result and intuitive analysis result obtain, at+60 DEG C of aseptic seedling stem apex (0.2cm-0.5cm)
It is best to manage 40min mode.
2, influence of the different heat treatment+cold treatment to ginger aseptic seedling proliferation rate and virus elimination rate
It after heat treatment grows fine, the healthy and strong aseptic seedling plant of leaf dark green is further transferred, each processing inoculation 8
Bottle, is placed on after cultivating 5 days in 5 DEG C of incubator, then be transferred in 25 DEG C of incubators and cultivate after inoculation by 4 plants every bottle, and remaining 2 bottles are not
By cold treatment, it is directly placed into culture in 25 DEG C of incubators and is used as control.Data are observed and recorded respectively after one week and 20 days, are calculated
The death rate and proliferation rate.
For the proliferative conditions of Jiang Miao, only in 9 combinations through Overheating Treatment and without cold treatment, combination 3
(A1B3C3D3) proliferation rate is up to 256.67%, is followed successively by 4 > of processing thereafter and handles 5 >, 7 > of processing processing 9, worst
It is processing 6, is 83.33%.Proliferation rate sequence is that 3 > of processing handle 5 > processing 4 >, 9 > of processing processing 2, processing 6 after cold treatment
Worst cultivation effect is 70%.In 9 processing after cold treatment, value-added effect most preferably handles 3, is 250.00%,
After be followed successively by processing 5 > handle 4 > handle 9 > processing 2, combination 6 proliferation rates it is minimum.Proliferation rate before and after comparative analysis cold treatment
Thus variation judges it can be found that Jiang Miao proliferation rate after cold treatment is declined, cold treatment to the normal growth of ginger with
Proliferation has certain inhibiting effect.The detoxification efficiency of 9 combinations (control) not Jing Guo cold treatment is widely different, handles 6 Hes
The virus elimination rate of processing 8 is up to 100.00%, followed by handles 1 and processing 9, the worst group of detoxification efficiency is combined into combination 5, removal efficiency
It is 0.In 9 processing by cold treatment, it is 33.33% that combination 2, combination 3, combination 4, combination 5, combination 7 are worst, processing
6, the virus elimination rate of processing 8 is still 100.00%, illustrates that combination 6 and combination 8 have been stripped of ralstonia solanacearum before cold treatment.Cause
This, optimal processing is combination 6 and combination 8.The data for comparing cold treatment and its control can be seen that except 5 virus elimination rates of processing improve
Outside 33.33%, virus elimination rate is essentially unchanged.Thus illustrate, 5 DEG C of cold treatment does not show the removal effect of ginger seedling diseases opportunistic pathogen
It writes.
The expanding propagation of 2 detoxicated ginger seedling of embodiment
1, by after detoxicated ginger seedling clip cauline leaf, its base portion is divided by simple bud, renewed vaccination contains 6-BA to new
It is cultivated in the MS culture medium of 0.5mg/L, KT 0.5mg/L and NAA 0.05mg/L and sucrose 15g/L, 23-26 DEG C of culture
40-45 days, obtain largely band root detoxicated ginger seedling.
2, by after detoxicated ginger seedling clip cauline leaf, its base portion is divided by simple bud, renewed vaccination contains 6-BA to new
It is cultivated in the MS culture medium of 1mg/L, KT 1.5mg/L and NAA 0.15mg/L and sucrose 20g/L, 23-26 DEG C of culture 40-
45 days, obtain largely band root detoxicated ginger seedling.
3, by after detoxicated ginger seedling clip cauline leaf, its base portion is divided by simple bud, renewed vaccination contains 6-BA to new
It is cultivated in the MS culture medium of 0.5mg/L, KT 1.2mg/L and NAA 0.05mg/L and sucrose 16g/L, 23-26 DEG C of culture
40-45 days, obtain largely band root detoxicated ginger seedling.
By being optimized to each component content in culture medium, it is known that the detoxicated ginger seedling growing way in the 3rd kind of situation is best.
The transplanting domestication of embodiment 3
It survives culture: single detoxicated ginger seedling similar in growing state that embodiment 2 obtains is planted respectively into 270 plastics
In basin, 2 plants of every basin is placed in 20-28 DEG C and cultivates 2 weeks, this incubation only needs to water, and is not required to other operations such as fertilising；Wherein,
Cultivation matrix is the mixture that perlite, peat soil and bio-bacterial manure are 1:3:0.5 mixing by volume.
Wherein, bio-bacterial manure comprises the following components in parts by weight: 2 parts of compound bacteria, 40 parts of corn flour, 15 parts of wheat bran, sucrose 3
Part, 28 parts of fulvic acid, 6 parts of ginkgo leaf, 0.2 part of ammonium molybdate, 0.06 part of zinc sulfate and 12 parts of water.
Compound bacteria comprises the following components in parts by weight: 1.2 parts of nitrogen-fixing bacteria, 1 part of phosphate solubilizing bacteria, 1.5 parts of potassium solubilizing bacteria, withered grass gemma
3 parts of bacillus.
Bio-bacterial manure is prepared by the following method to obtain: ginkgo leaf crushed, it is then mixed with corn flour, wheat bran and suitable quantity of water
It is even, compound bacteria is then added, stirs evenly, then adds fulvic acid and excess water, mixes, is subsequently placed in 30 DEG C of fermentations 20
It, is made.
Culture early period (after cultivation the 3-14 weeks): applying fertilizer to the detoxic seedling after surviving, and every basin applies fertilizer (nitrogen, phosphorus and potassium)
150mL, fertilising is primary weekly, and when fertilising takes base portion to pour mode；Monthly microelement of foliage-spray simultaneously, in this phase
Between suitably watered according to matrix dry and wet situation, and monthly carry out an insect pest preventing and controlling.
Wherein, 3 horizontal N1, N2 and N3 of N element are 60,120 and 180mg/L respectively；Corresponding 3 levels of P element
P1, P2 and P3 are 20,60 and 100mg/L respectively；K element corresponding 3 horizontal K1, K2 and K3 are 100,200 and respectively
300mg/L。
Early period, the influence of N, P and K the results are shown in Table 4-6.
Later period culture (after cultivation the 15-26 week): the process with early period culture compared be only the concentration of applied nitrogen, phosphorus and potassium not
Together, remaining is consistent with incubation early period.
Later period mainly increases the topdressing amount to N, K fertilizer, and 3 horizontal N1, N2 and N3 of N element setting are 200,250 respectively
And 300mg/L；K element corresponding 3 horizontal K1, K2 and K3 are 200,400 and 600mg/L respectively.
The influence of later period N, P and K the results are shown in Table 7-8.
Table 4 difference N, P, K are with the influence for comparing aerial growth detoxicated ginger seedling early period
As shown in Table 4, with the increase of N, P, K amount of application, detoxicated ginger seedling grows branch amount early period, plant height, newly-increased leaf
The piece number and blade rate of rise are on a declining curve on the whole.Influence to branch amount and plant height is processing 1 (N1P1K1) compared with
Good, every plant averagely increases 6 branches newly, and plant height increases 18.21cm；The newly-increased number of blade and blade rate of rise are processing 2
(N1P2K2) preferably, single-strain blade increases by 32.6 pieces/plant, during measurement blade rate of rise be 5.43/(strain week), processing
8 (N3P2K3) are poor, 2.3/plant of branch amount average out to, and plant height amplification is minimum, only increase 0.66cm, the number of blade is minimum, only increases
Add 1.3 pieces/plant, during measurement blade rate of rise be only 0.22/(strain week)；Remaining processing is therebetween.To sum up
Think, detoxicated ginger seedling grows that demand of the early period to N, P, K is relatively fewer, and higher concentration is unfavorable for tissue-cultured seedling growth instead, applies
Fertilizer processing 1 and 2 i.e. N60mg/L, P20mg/L, K100mg/L and N60mg/L, P60mg/L, K200mg/L combination are preferable.
5 different fertilization of table grows the influence of stem early period fresh weight and rhizome number to ginger
| Processing number | Fresh weight/g | Rhizome number/piece |
| 1=N1P1K1 | 41.20 | 8.40 |
| 2=N1P2K2 | 33.34 | 7.93 |
| 3=N1P3K3 | 26.99 | 7.93 |
| 4=N2P1K3 | 25.22 | 7.00 |
| 5=N2P2K1 | 14.37 | 5.43 |
| 6=N2P3K2 | 11.29 | 5.20 |
| 7=N3P1K2 | 14.71 | 4.80 |
| 8=N3P2K3 | 7.49 | 3.93 |
| 9=N3P3K1 | 14.34 | 4.53 |
As shown in Table 5, detoxicated ginger seedling grows early period with the increase of N, P, K amount of application, newborn rhizome fresh weight and rhizome
Downward trend is presented in number on the whole.Wherein harvest fresh weight and rhizome number it is maximum be processing 1, harvest fresh weight 41.20g, newly
8.40 pieces of stem number of taking root；Harvesting the smallest is processing 8, harvests fresh weight only 7.49g, 3.93 pieces of new root stem number, remaining processing is situated between
In between the two.
6 different fertilization of table grows the intuitive analysis of rhizomes early period fresh weight and rhizome number to ginger
As shown in Table 6, the size relation for influencing the factor of newborn rhizome fresh weight and rhizome number is N > P > K.By these three
Factor carries out variance analysis, the equal < F0.05 of F value, which illustrates that difference is not significant.Because the freedom degree of three is identical, pass through F
Value determines that their size relation is N > P > K, i.e., in the early period of detoxicated ginger seedling growth, N is to ginger fresh weight and rhizome number
Maximum is influenced, P takes second place, and K is worst, is consistent as the result is shown with intuitive analysis (N > P > K).It can be seen that processing from the test result
The increment of 1 rhizome fresh weight is maximum, and rhizome number is most.To sum up, 1 (N1P1K1) of processing is to detoxicated ginger seedling rhizome early period fresh weight and rhizome
Number increases preferably, i.e. N60mg/L, P20mg/L, K100mg/L combination is preferable.
Influence of 7 different fertilization of table to ginger Later growth stem fresh weight and rhizome number
| Processing number | Fresh weight/g | Rhizome number/piece |
| 1=N1K1 | 38.673 | 10.53 |
| 2=N1K2 | 23.814 | 7.93 |
| 3=N1K3 | 27.928 | 8.36 |
| 4=N2K3 | 35.644 | 10.53 |
| 5=N2K1 | 28.931 | 8.40 |
| 6=N2K2 | 34.266 | 10.33 |
| 7=N3K2 | 34.316 | 10.27 |
| 8=N3K3 | 28.658 | 9.13 |
| 9=N3K1 | 27.406 | 9.57 |
As shown in Table 7, with the increase of N, K amount of application, detoxicated ginger seedling Later growth rhizome fresh weight and rhizome number are whole
On downward trend is presented.Wherein fresh weight value added is maximum for processing 1, weight gain 38.673g；It increases weight the smallest to handle 2, only
Increase weight 23.814g, remaining processing is therebetween；Rhizome number it is more be processing 1, rhizome number 10.53, less is place
Reason 2, only 7.93.
Intuitive analysis of 8 different fertilization of table to ginger Later growth stem fresh weight and rhizome number
As shown in Table 8, ginger Later growth rhizome fresh weight and rhizome number intuitive analysis shows, influence the increase of rhizome fresh weight
Factor size relation with rhizome number is N > K.The two factors are subjected to variance analysis, the equal < F0.05 of F value, which says
Bright difference is not significant.Because the freedom degree of the two is identical, determine that their size relation is N > K by F value, i.e., in detoxicated ginger
The later period of seedling growth, influence of the N to rhizome fresh weight and rhizome number are greater than K, are consistent as the result is shown with intuitive analysis (N > K).From this
Test result can be seen that the increment of processing 1 (N1K1) rhizome fresh weight is maximum, and rhizome number is most.Therefore, 1 (N1K1) of processing is to ginger
Detoxic seedling later period rhizome fresh weight and rhizome number increase preferably, i.e. N200mg/L, K200mg/L combination is preferable.
In conclusion growing early period, comprehensive mean leaf rate of rise, plant height and tiller number and ground in detoxicated ginger
The test result of lower part rhizome fresh weight and rhizome number think N, P, K optimal fertilization mode be N60mg/L, P20mg/L,
K100mg/L [ρ (N): ρ (P): ρ (K)=3:1:5].It can be seen that detoxicated ginger seedling growth fertilizer requirement early period is relatively fewer,
Shaoshi is answered in production process.Potassium acts on obviously the growth and development of ginger, and the appropriate Potassium Fertilizer that increases can reach significant volume increase
Effect, the increment of rhizome substantially 11.7~31.8%, average rate of growth is up to 14.85%.This test result shows in detoxification
It is N200mg/L, K200mg/L that seedling Later growth, which most preferably applies fertilizer,.N, the amount of application of K is significantly improved relative to early period.
Embodiment 4
The present embodiment difference from Example 3 is that cultivation matrix is that volume is pressed in perlite, peat soil and bio-bacterial manure
Than the mixture mixed for 1:3:0.3, remaining parameter is the optimized parameter in embodiment 3.
Embodiment 5
The present embodiment difference from Example 3 is that cultivation matrix is that volume is pressed in perlite, peat soil and bio-bacterial manure
Than the mixture mixed for 2:5:0.3, remaining parameter is the optimized parameter in embodiment 3.
Embodiment 6
The present embodiment difference from Example 3 is, 1 part of compound bacteria, 30 parts of corn flour, 10 parts of wheat bran, 2 parts of sucrose,
20 parts of fulvic acid, 5 parts of ginkgo leaf, 0.1 part of ammonium molybdate, 0.05 part of zinc sulfate and 8 parts of water, remaining parameter are in embodiment 3
Optimized parameter.
Embodiment 7
The present embodiment difference from Example 3 is, 3 parts of compound bacteria, 40 parts of corn flour, 20 parts of wheat bran, 4 parts of sucrose,
30 parts of fulvic acid, 10 parts of ginkgo leaf, 0.3 part of ammonium molybdate, 0.08 part of zinc sulfate and 15 parts of water, remaining parameter are in embodiment 3
Optimized parameter.
Embodiment 8
The present embodiment difference from Example 3 is, does not include compound bacteria, remaining parameter is optimal in embodiment 3
Parameter.
These embodiments of embodiment 3 (preferred parameter)-embodiment 8 cultivation detoxicated ginger seedling, embodiment 4-8 either from
Aerial part or under ground portion are poorer than embodiment 3, and survival rate and yield are below embodiment 3, embodiment 4-7 and implementation
3 difference degree of example is not very big, but embodiment 8 is obviously poorer than embodiment 3.
In conclusion preparing detoxic seedling and the later period expansion proliferation and transplanting domestication, whole process in the present invention
It is an entirety, virus elimination rate height is just prepared in the combination of all parameters, and survival rate is high, and yield is high, the short ginger of growth cycle.
Claims (9)
1. a kind of detoxicated ginger mating system, which comprises the following steps:
(1) preparation of detoxicated ginger seedling
The young shoot for taking ginger rhizomatic germination peels off outer layer leaf sheath, clean with aseptic water washing, then carries out disinfection, then takes
0.2-0.5cm shoot tip meristem access containing 6-BA 0.5-1mg/L, KT 0.5-1.5mg/L, NAA 0.05-0.15mg/L and
In the MS culture medium of sucrose 15-20g/L, it is placed in 60 DEG C of heat treatment 40min, then under dark condition, 23-26 DEG C of culture 2-3
It is 23 ± 1 DEG C that day temperature is transferred to after it, and nocturnal temperature is 18 ± 1 DEG C, intensity of illumination 2000-3000lx, and light application time is
It is cultivated 28-30 days under the conditions of 12-14h/ days, obtains regeneration tissue-cultured seedling, then carry out viral diagnosis, filter out detoxicated ginger seedling；
(2) expanding propagation of detoxicated ginger seedling
After the detoxicated ginger seedling that step (1) obtains is cut off cauline leaf, its base portion is divided by simple bud, renewed vaccination contains to new
It is trained in the MS culture medium of 6-BA 0.5-1mg/L, KT 0.5-1.5mg/L and NAA 0.05-0.15mg/L and sucrose 15-20g/L
It supports 40-45 days, obtains largely band root detoxicated ginger seedling；
(3) transplanting domestication
It survives culture: a large amount of detoxicated ginger seedlings that step (2) obtain being planted in cultivation matrix, 20-28 DEG C of culture 1-2 is placed in
Week；Wherein, cultivation matrix is the mixing that perlite, peat soil and bio-bacterial manure are 1-2:3-5:0.3-0.8 mixing by volume
Object；
Culture early period: applying fertilizer to the detoxic seedling after surviving, every 2 plants of fertilisings 150-170mL, the concentration difference of nitrogen, phosphorus and potassium
For 60-100mg/L, 20-40mg/L and 100-150mg/L, fertilising is primary weekly；
Later period culture: after detoxic seedling is grown 13-14 weeks, every 2 plants of fertilisings 150-170mL, the concentration of nitrogen, phosphorus and potassium is respectively 200-
220mg/L, 20-40mg/L and 200-250mg/L, fertilising is primary weekly；
A leaf surface trace fertilizer is monthly sprayed in culture early period and later period incubation, while monthly carrying out an insect pest preventing and controlling.
2. detoxicated ginger mating system according to claim 1, which is characterized in that used in step (1) neutralization procedure (2)
Culture medium is the MS culture medium containing 6-BA 0.5mg/L, KT 1.2mg/L and NAA 0.05mg/L and sucrose 16g/L.
3. detoxicated ginger mating system according to claim 1, which is characterized in that bio-bacterial manure includes following parts by weight
Component: 1-3 parts of compound bacteria, 30-40 parts of corn flour, 10-20 parts of wheat bran, 2-4 parts of sucrose, 20-30 parts of fulvic acid, ginkgo leaf 5-
10 parts, 0.1-0.3 parts of ammonium molybdate, 0.05-0.08 parts of zinc sulfate and 8-15 parts of water.
4. detoxicated ginger mating system according to claim 3, which is characterized in that bio-bacterial manure includes following parts by weight
Component: 2 parts of compound bacteria, 40 parts of corn flour, 15 parts of wheat bran, 3 parts of sucrose, 28 parts of fulvic acid, 6 parts of ginkgo leaf, ammonium molybdate 0.2
Part, 0.06 part of zinc sulfate and 12 parts of water.
5. detoxicated ginger mating system according to claim 3 or 4, which is characterized in that compound bacteria includes following parts by weight
Component: 1-2 parts of nitrogen-fixing bacteria, 1-2 parts of phosphate solubilizing bacteria, 1-2 parts of potassium solubilizing bacteria, 2-4 parts of bacillus subtilis.
6. detoxicated ginger mating system according to claim 5, which is characterized in that compound bacteria includes the group of following parts by weight
Point: 1.2 parts of nitrogen-fixing bacteria, 1 part of phosphate solubilizing bacteria, 1.5 parts of potassium solubilizing bacteria, 3 parts of bacillus subtilis.
7. detoxicated ginger mating system according to claim 3 or 4, which is characterized in that bio-bacterial manure is by the following method
It is prepared:
Ginkgo leaf is crushed, is then mixed with corn flour, wheat bran and suitable quantity of water, compound bacteria is then added, stirs evenly, then again
Fulvic acid and excess water is added, mixes, is subsequently placed in 30-35 DEG C of fermentation 15-20 days, is made.
8. detoxicated ginger mating system according to claim 1, which is characterized in that every 2 in step (3) incubation early period
Strain fertilising 150mL, the concentration of nitrogen, phosphorus and potassium is respectively 60mg/L, 20mg/L and 100mg/L.
9. detoxicated ginger mating system according to claim 1, which is characterized in that every 2 in step (3) later period incubation
Strain fertilising 150mL, the concentration of nitrogen, phosphorus and potassium is respectively 200mg/L, 20mg/L and 200mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711001036.1A CN107529398B (en) | 2017-10-24 | 2017-10-24 | A kind of detoxicated ginger mating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711001036.1A CN107529398B (en) | 2017-10-24 | 2017-10-24 | A kind of detoxicated ginger mating system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107529398A CN107529398A (en) | 2018-01-02 |
| CN107529398B true CN107529398B (en) | 2019-05-07 |
Family
ID=60766464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711001036.1A Expired - Fee Related CN107529398B (en) | 2017-10-24 | 2017-10-24 | A kind of detoxicated ginger mating system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107529398B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI712361B (en) * | 2018-06-01 | 2020-12-11 | 謝瑞裕 | Method of ginger plant cultivation |
| CN110192524B (en) * | 2019-06-13 | 2022-03-08 | 仲恺农业工程学院 | Method for in vitro rapid breeding of Zingiberaceae plant by using leaf stem and inflorescence stem hidden bud as explant |
| CN110896838A (en) * | 2019-12-06 | 2020-03-24 | 神州姜窖农业集团有限公司 | Soilless culture method for detoxified ginger seedlings |
| CN111670808A (en) * | 2020-07-07 | 2020-09-18 | 台州市农业科学研究院 | Cultivation method of purple bud dancing flower ginger seedlings |
| CN112931114A (en) * | 2021-03-17 | 2021-06-11 | 永州职业技术学院 | High-yield and high-efficiency cultivation method for original ginger |
| CN113598000B (en) * | 2021-08-27 | 2022-08-30 | 湖北省农业科学院经济作物研究所 | Method for one-step formation of ginger rootless tissue culture seedlings into field seedlings |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1930951B (en) * | 2006-09-29 | 2010-12-01 | 徐坤 | Method of cultivating detoxicated ginger seedling |
-
2017
- 2017-10-24 CN CN201711001036.1A patent/CN107529398B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN107529398A (en) | 2018-01-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107529398B (en) | A kind of detoxicated ginger mating system | |
| CN108753676A (en) | A kind of selection of Stropharia rugoso-annulata high-temperature resistant strain | |
| CN110052485A (en) | A method of strengthen to extract using rape-Sedum alfredii Hance intercropping and interplanting and repairs cadmium mild or moderate polluted agricultural land | |
| CN110106094A (en) | High temperature resistant Stropharia rugoso-annulata bacterial strain and its application | |
| CN101124892B (en) | Cymbidium edaphic orchids seed aseptic seeding growing seedlings method | |
| CN116478870A (en) | Maltophilous oligotrophic single spore fungus OLR3-17 strain and application thereof | |
| CN101011028B (en) | Breeding method of chrysanthemum haploid | |
| Roy et al. | β-N-Oxalyl-L-α, β-diaminopropionic acid in somaclones derived from internode explants of Lathyrus sativus | |
| CN100557015C (en) | A kind of nodule azotobacter strain is BXBL9 and application thereof | |
| CN109673469A (en) | A kind of preparation method with growth promotion seedling medium | |
| CN105349432A (en) | Puccinia polysora underw single-spore propagation method | |
| CN110476807B (en) | Method for establishing sterile culture system of mature seed embryo of peony 'paeonia ostii' for oil | |
| CN109122123B (en) | Method for constructing highland barley-endophytic fungi symbiont | |
| CN116574649A (en) | Lignin degrading bacterium M15H2 with growth promoting, disease resisting and phosphorus dissolving capabilities and application thereof | |
| CN107173225B (en) | The method for carrying out androgenesis with Sweet Potato Leaf | |
| CN115975817A (en) | Ganoderma lucidum strain L5478 and artificial cultivation method thereof | |
| CN104620983A (en) | Method for forming seedlings by carrying out tissue culture on echinochloacrus-galli | |
| CN105948889B (en) | Artificial culture truffle Va Mycorrhiza Seedling is inoculated with specific complex agent and its preparation method and application method | |
| CN101692787B (en) | Method for collecting and culturing ulva spores | |
| CN112243856A (en) | Early-maturing late-japonica rice hybrid breeding method | |
| CN109197504A (en) | A kind of sugarcane test tube seedling seedling nutritious soil and its preparation method and application | |
| CN112893453B (en) | Method for repairing cadmium-polluted soil by intercropping corn and wedelia chinensis and inoculating AMF | |
| CN104221851A (en) | Method for in-vitro culture and rapid propagation of gunnera manlcata l | |
| CN115281078B (en) | Method for inducing iris polyploid by colchicine | |
| CN114698512B (en) | Application of nano cerium oxide in promoting plant lateral root occurrence |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190507 Termination date: 20191024 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |