CN109197504A - A kind of sugarcane test tube seedling seedling nutritious soil and its preparation method and application - Google Patents
A kind of sugarcane test tube seedling seedling nutritious soil and its preparation method and application Download PDFInfo
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- CN109197504A CN109197504A CN201810996927.3A CN201810996927A CN109197504A CN 109197504 A CN109197504 A CN 109197504A CN 201810996927 A CN201810996927 A CN 201810996927A CN 109197504 A CN109197504 A CN 109197504A
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- test tube
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention relates to a kind of sugarcane test tube seedling seedling nutritious soils and its preparation method and application, the Nutrition Soil includes weathering pond sludge, river sand and phosphate fertilizer, wherein, the quality proportioning of the weathering pond sludge and river sand is 1:(0.8-1.2), and phosphorus content is 0.23%-0.33% in the Nutrition Soil；Nutrition Soil of the present invention can promote the root system development of sugarcane test tube seedling, and optimal growing environment and nutriment are improved for sugarcane test tube seedling, significantly improve the survival rate of test tube transplantation of seedlings, and component is simple, cost is relatively low.
Description
Technical field
The present invention relates to sugarcane Test tube seedlings technical fields, in particular to a kind of sugarcane test tube seedling seedling nutritious soil
And its preparation method and application.
Background technique
Plant test tube transplantation of seedlings is a key link in Plant Tissue Breeding process, plant test tube transplantation of seedlings survival rate
Height with it is low be measure plant tissue culture technique success with failure key index, and determine Plant Tissue Breeding it is high-efficient
With low key index.Common plant test tube seedling matrix has coarse vermiculite, perlite, rough sand, boiler ash sediment, husk, saw at present
Sawdust, fertile soil, native garden mould etc. can also mix in proportion two or more above matrix according to floristics characteristic
It uses.
Sugarcane, (scientific name: Saccharum officinarum) saccharum, perennial tall and big solid draft.Rhizomes is sturdy
It is flourishing.High 3-5 (- 6) rice of stalk.Extensive kind of the torrid areas in the south such as TaiWan, China, Fujian, Guangdong, Hainan, Guangxi, Sichuan, Yunnan
It plants.Sugarcane is suitble to plant in fertile soil, place sunny, summer in the winter temperature difference is big.
Currently, since there are certain defects for substrate preparation, causing test tube transplantation of seedlings during sugarcane test tube transplantation of seedlings
When, root system development is unhealthy, and survival rate is low, and seedling cost is higher.
In view of this, the present invention is specifically proposed.
Summary of the invention
The first object of the present invention is to provide a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil can promote sweet
The root system development of sugarcane test tube seedling improves the survival rate of test tube transplantation of seedlings, and component is simple, and cost is relatively low.
The second object of the present invention is to provide a kind of preparation method of Nutrition Soil, and this method is easy to operate, and
It can promote the content of effective component in pond sludge, and be convenient for the absorption of sugarcane test tube seedling.
The third object of the present invention is to provide a kind of application of the Nutrition Soil in sugarcane test tube seedling method for culturing seedlings, should
Using the transplanting survival rate that can be improved sugarcane test tube seedling.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
The first aspect of the present invention is to provide a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil includes the weathering pool
Mud, river sand and phosphate fertilizer, wherein the weathering pond sludge is obtained by carrying out weathering to fresh pond sludge, weathering pond sludge and river sand
Quality proportioning be 1:(0.8-1.2), phosphorus content is 0.23-0.33% in the Nutrition Soil.
Further, in the Nutrition Soil nitrogen content be 0.35%-0.45%, potassium content 0.75%-0.95%；
Preferably, the Ph of the Nutrition Soil is 5.5-6.5, and preferably Ph is 6.15-6.24, specific gravity 2.60-2.70.
The present invention is combined by above-mentioned specified raw material, and nutrient abundant can be provided for sugarcane test tube seedling and is suitable for
Growing environment is conducive to sugarcane test tube seedling and grows, and promotes the root system development of sugarcane test tube seedling, improves the survival of test tube transplantation of seedlings
Rate reduces seedling cost；In addition, passing through the specific restriction of phosphate fertilizer content, the root system development of test tube seedling can be promoted, improve sugarcane
The survival rate of test tube transplantation of seedlings.
Further, the weight proportion of the weathering pond sludge and river sand is 1: 1；The phosphate fertilizer content is 0.28%.
It is combined by above-mentioned specific proportion, optimal growing environment and optimal concentration can be provided for sugarcane test tube seedling
Nutriment can significantly improve the survival rate of sugarcane test tube seedling.
The source of weathering pond sludge is not limited strictly in the present invention, it is preferable that the weathering pond sludge may come from
Pond, river embankment, any one in lotus pool；It is highly preferred that the weathering pond sludge partial size is 0.02-3mm；The weathering pond sludge contains
Water is 8%-15%.
By the specific restriction of above-mentioned weathering pond sludge partial size, above-mentioned Nutrition Soil is enabled to be conducive to the nutrition of sugarcane test tube seedling
It absorbs, and with good aeration and suitable water retention property, the growth and development of sugarcane test tube seedling can be promoted.
River sand be natural stone in its natural state, active force through water collides repeatedly for a long time, rubbing generates, composition
There are certain slickness, the non-metallic ore more than impurity content in complex, surface.
River sand type is not limited strictly in the present invention, it is preferable that the river sand is fresh river sand；It is highly preferred that
The partial size of the river sand is 0.02-2mm.
By the specific selection of river sand partial size, the aeration and water retention property of the Nutrition Soil can be improved, tried for sugarcane
Pipe seedling provides optimal growing environment.
The type of phosphate fertilizer is not limited strictly in the present invention, it is preferable that the phosphate fertilizer is calcium superphosphate and/or calcium and magnesium
Phosphate fertilizer.
By the selection of above-mentioned specific phosphate fertilizer, it can be more conducive to the absorption of sugarcane test tube seedling, conducive to the growth of sugarcane test tube seedling
And survival.
The second aspect of the present invention provides a kind of preparation method of above-mentioned Nutrition Soil, includes the following steps:
(1) weathering is carried out to fresh pond sludge, obtains weathering pond sludge；
(2) weathering pond sludge and river sand are mixed to get mixture；
(3) phosphate fertilizer is added in mixture, is uniformly mixed so as to obtain the Nutrition Soil；
It preferably, further include being crushed to weathering pond sludge in the step (1).
By carrying out weathering processing to fresh pond sludge, the effective component in pond sludge can be improved, and remove in pond sludge
The toxicant of harmful bacteria and reproducibility；In addition, the Nutrition Soil being prepared by the above method, can be sugarcane test tube seedling
Nutrient needed for growing and suitable growing environment are provided, conducive to the growth and survival of sugarcane test tube seedling.
Further, the weathering is that fresh pond sludge is carried out heavy natural sunning；The time of being exposed to the sun is 90 days or more；It is described
It is crushed to 0.02-3mm；
Preferably, the time of being exposed to the sun is 90-150 days；It is described to be crushed to 0.02-1mm.
The third aspect of the present invention provides a kind of method for culturing seedlings of sugarcane test tube seedling, and this method is carried out using above-mentioned Nutrition Soil
Culture.By the selection of above-mentioned Nutrition Soil, the survival rate of test tube seedling can be improved.
Further, the method for culturing seedlings includes the following steps:
(1) shoot proliferation cultivation cycle will be completed, and the unrooted sugarcane seedling bottle containing subculture multiplication medium is put in cool place, keeps away
At wind, foliage-spray liquid is sprayed, then, carries out hardening treatment；
(2) test tube seedling after taking out hardening in bottle, is cleaned and is removed old leaf；
(3) test tube seedling after cleaning is carried out disinfection；
(4) test tube seedling after disinfection is planted in the planting pot for being placed with above-mentioned Nutrition Soil, is watered with root water；
(5) planting pot is placed in seedling cultivation greenhouse, and covers sunshade net, until 90% test tube seedling grows young leaves, open screening
Screened postive adjusts seedling cultivation greenhouse environment, and is spaced and carries out water drenching processing, until test tube seedling is out of the garden.
It is closed by above-mentioned specific method for culturing seedlings and specific nutrition local soil type, sugarcane test tube seedling can be significantly improved in transplanting process
In survival rate.
Preferably, the page spray liquid be IBA20mg/L, NAA10mg/L, sucrose 20g/L, L-Pro 100mg/L,
Hydroxyisoxazole 400mg/L, tween 2ml/L；
Preferably, the hardening treatment is, at 20-30 DEG C of temperature, humidity 65%-80%, intensity of illumination 2000-
Under 10000lux, hardening 24-26h；
Preferably, the disinfection is 8-10min in the bright shield solution for immerse test tube seedling 500 times；
Preferably, the air humidity of the seedling rearing room is 40%-90%, intensity of illumination 2000-10000lux；
Preferably, adjusting nursery room environmental is that air humidity is adjusted to 68%-72%；
Preferably, the interval carries out water drenching processing to carry out water drenching 28-32s at interval of 25-35min.
Compared with prior art, beneficial effects of the present invention include at least:
(1) Nutrition Soil of the present invention can promote the root system development of sugarcane test tube seedling, improve the survival of test tube transplantation of seedlings
Rate, and component is simple, and cost is relatively low.
(2) preparation method of Nutrition Soil of the present invention is easy to operate, and can promote containing for effective component in pond sludge
Amount, convenient for the absorption of sugarcane test tube seedling.
(3) it can be improved in Nutrition Soil of the present invention by the specific dosage of raw material for sugarcane test tube seedling optimal
Growing environment and nutriment significantly improve the survival rate of test tube transplantation of seedlings.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products obtained can be bought by city.
The raw material that following embodiment uses is as follows:
Fresh pond sludge: piscina is derived from；
Fresh river sand: partial size 0.02-2mm；From freshet.
Embodiment 1
One, sugarcane test tube seedling seedling nutritious soil
Above-mentioned Nutrition Soil is made of weathering pond sludge, fresh river sand and phosphate fertilizer, wherein the weight of weathering pond sludge and fresh river sand
Proportion is 1: 0.8；Phosphorus content is 0.23% in the Nutrition Soil；
Weathering pond sludge partial size is 0.02-3mm；
Phosphate fertilizer is calcium superphosphate.
Two, preparation method
The preparation method of above-mentioned Nutrition Soil, includes the following steps:
(1) fresh pond sludge is carried out heavy natural sunning 150 days, is subsequently broken up to 0.02-3mm；
(2) above-mentioned broken pond sludge and fresh river sand are mixed to get mixture；
(3) calcium superphosphate is added in said mixture, is uniformly mixed so as to obtain above-mentioned Nutrition Soil.
Using domestic forest soil industry examination criteria LY/T1228-2015, LY/T1232-2015 and LY/1234-2015
Method detects nitrogen, phosphorus, the potassium in the Nutrition Soil of above-mentioned preparation, and testing result is nitrogen content 0.43%, phosphorus content
0.23%, potassium content 0.88%.
Embodiment 2
One, sugarcane test tube seedling seedling nutritious soil
Above-mentioned Nutrition Soil is made of pond sludge, fresh river sand and phosphate fertilizer, wherein the weight proportion of pond sludge and fresh river sand is 1:
1.2；Phosphorus content is 0.33% in the Nutrition Soil；
Weathering pond sludge partial size is 0.02-3mm；
Phosphate fertilizer is calcium magnesium phosphate.
Two, preparation method
The preparation method of above-mentioned Nutrition Soil, includes the following steps:
(1) fresh pond sludge is carried out heavy natural sunning 150 days, is subsequently broken up to 0.02-3mm；
(2) above-mentioned broken pond sludge and fresh river sand are mixed to get mixture；
(3) calcium magnesium phosphate is added in said mixture, is uniformly mixed so as to obtain above-mentioned Nutrition Soil.
The mass parameter of the Nutrition Soil of above-mentioned preparation is detected using the method for embodiment 1, testing result is nitrogen
Content 0.4%, phosphorus content 0.33%, potassium content 0.81%.
Embodiment 3
One, sugarcane test tube seedling seedling nutritious soil
Above-mentioned Nutrition Soil is made of pond sludge, fresh river sand and calcium superphosphate, wherein the weight proportion of pond sludge and fresh river sand
It is 1: 1；Phosphorus content is 0.28% in the Nutrition Soil；
Weathering pond sludge partial size is 0.02-3mm；
Phosphate fertilizer is calcium superphosphate.
Two, preparation method
The preparation method of above-mentioned Nutrition Soil, includes the following steps:
(1) fresh pond sludge is carried out heavy natural sunning 90 days, subsequently breaks up 0.02-3mm；
(2) above-mentioned broken pond sludge and fresh river sand are mixed to get mixture；
(3) calcium superphosphate is added in said mixture, is uniformly mixed so as to obtain above-mentioned Nutrition Soil.
The mass parameter of the Nutrition Soil of above-mentioned preparation is detected using the method for embodiment 1, testing result is nitrogen
Content 0.41%, phosphorus content 0.28% and potassium content 0.85%.
Embodiment 4
The present embodiment is a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil and the Nutrition Soil of embodiment 3 are essentially identical,
Difference is only that the partial size of weathering pond sludge is 3.5-4.5mm.The preparation method of the Nutrition Soil and the preparation method phase in embodiment 3
Together.
Embodiment 5
The present embodiment is a kind of sugarcane test tube seedling method for culturing seedlings, is included the following steps:
(1) shoot proliferation cultivation cycle will be completed, and the unrooted sugarcane seedling bottle containing subculture multiplication medium is put in cool place, keeps away
At wind, foliage-spray liquid is sprayed, then, carries out hardening treatment；
Wherein, the foliage-spray liquid is IBA20mg/L, NAA10mg/L, sucrose 20g/L, L-Pro 100mg/L, soil
Bacterium disappears 400mg/L, tween 2ml/L；The hardening treatment is, at 25 DEG C of temperature, humidity 70%, and under intensity of illumination 6000lux, refining
Seedling is for 24 hours；
(2) test tube seedling after taking out hardening in bottle, is cleaned and is removed old leaf；
(3) test tube seedling after cleaning is immersed to 9min in 500 times of bright shield solution, is carried out disinfection；
(4) test tube seedling after disinfection is planted in being placed in embodiment 1-3 any embodiment in the planting pot of Nutrition Soil, is poured
Sufficient root water；
(5) above-mentioned planting pot is placed in seedling cultivation greenhouse, control seedling rearing room air humidity is 60%, and intensity of illumination is
4000lux；And sunshade net is covered, until 90% test tube seedling grows young leaves, sunshade net is opened, adjusting air humidity is educated for 70%
Seedling greenhouse, and it is spaced 30min water drenching 30s, until test tube seedling is out of the garden.
Reference examples 1
This reference examples is a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil and the Nutrition Soil of embodiment 3 are essentially identical,
Difference, which is only that, is not added with phosphate fertilizer.The preparation method of the Nutrition Soil is identical as the preparation method in embodiment 3.
The mass parameter of the Nutrition Soil of above-mentioned preparation is detected using the method for embodiment 1, testing result is to survey
It as a result is nitrogen content 0.42%, phosphorus content 0.14% and potassium content 0.88%.
Reference examples 2
This reference examples is a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil and the Nutrition Soil of embodiment 3 are essentially identical,
Difference is only that the weathering pond sludge that river sand is substituted for equivalent weight.The preparation method of the Nutrition Soil and the preparation in embodiment 3
Method is identical.
Reference examples 3
This reference examples is a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil and the Nutrition Soil of embodiment 3 are essentially identical,
Difference is only that the marsh silt that pond sludge is substituted for equivalent weight.The preparation method of the Nutrition Soil and the preparation in embodiment 3
Method is identical.
Reference examples 4
This reference examples is a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil and the Nutrition Soil of embodiment 3 are essentially identical,
Difference is only that the yellow mud that pond sludge is substituted for equivalent weight is husky.The preparation method of the Nutrition Soil and the preparation side in embodiment 3
Method is identical.
Reference examples 5
This reference examples is a kind of sugarcane test tube seedling seedling nutritious soil, and the Nutrition Soil and the Nutrition Soil of embodiment 3 are essentially identical,
Difference is only that the disinfection rural area soil that pond sludge is substituted for isodose is husky；Sterilizing rural area soil sand is by 121 DEG C, 0.105MPa
Under conditions of disinfection 15-20min obtain.The preparation method of the Nutrition Soil is identical as the preparation method in embodiment 3.
Experimental example
This experimental example is detection sugarcane test tube seedling during transplanting, influence of the Different Nutrition soil to test tube seedling survival rate.
Experimental material is the sugared No. 49 proliferation and subculture seedlings (unrooted) in osmanthus.
Experiment flow is as follows:
(1) hardening: shoot proliferation cultivation cycle will be completed, and the unrooted sugarcane seedling bottle containing subculture multiplication medium is put in yin
Cool, lee opens bottle cap, sprays the foliage-spray liquid of following formulas, spray liquid composition are as follows: IBA20mg/L, NAA10mg/L,
Sucrose 20g/L, L-Pro 100mg/L, hydroxyisoxazole 400mg/L, tween 2ml/L；Then at 25 DEG C of temperature, humidity 60%, illumination
Under intensity 6000lux, hardening 24 hours；
(2) divide test tube seedling: taking out test tube seedling after hardening from bottle, clean culture medium with clear water, remove old leaf；
(3) it sterilizes: 500 times of bright shield solution of the test tube seedling after segmentation cleaning is impregnated into 9min；
(4) it plants: the test tube seedling after disinfection being divided into single plant or little Cong plantation in the planting pot for being placed with cultivation matrix, is poured
Sufficient root water；Cultivation matrix is respectively the Nutrition Soil in embodiment 1-4, reference examples 1-5；Every kind of Nutrition Soil plants 6 kinds respectively
Basin is planted, each planting pot specification is 9 × 6 holes.
(5) it takes root cultivating and managing: the planting pot with test tube seedling is moved into the seedling cultivation greenhouse of automatic adjustment temperature humidity, lid
Upper sunshade net adjusts seedling cultivation greenhouse environment: greenhouse air range of humidity variation 40%-90%, intensity of illumination 2000-
10000lux；After 90% sugarcane test tube seedling grows young leaves, sunshade net is opened, chamber humidity control is 70%, at interval of 30
Water drenching 30 seconds minute, until test tube seedling is out of the garden；After test tube seedling grows young leaves, start applying liquid fertilizer and weeding, nursery bed management and often
It is identical to advise sugarcane test tube seedling method for culturing seedlings.
(6) investigate survival rate: each planting pot survival hole number, survival rate (%)=(test tube seedling are investigated in plantation after 17 days
Survival hole number/54) × 100%, statistics obtains the survival rate of sugarcane unrooted test tube seedling in each cultivation matrix, referring to table 1.
The sugarcane unrooted test tube seedling survival rate of 1 test cultivation matrix of table
As shown in Table 1
By it is above-mentioned be the comparison of strength and reference examples it is found that using the Nutrition Soil of the embodiment of the present application 1-3 as cultivation
Matrix can significantly improve sugarcane unrooted test tube seedling survival rate, and survival rate reaches 97% or more, however, changing Nutrition Soil
The change of the change of partial size and nutriment, especially phosphorus content reduces the survival rate of sugarcane test tube seedling.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Present invention has been described in detail with reference to the aforementioned embodiments for pipe, but those skilled in the art should understand that: its
It is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features
It is equivalently replaced；And these are modified or replaceed, various embodiments of the present invention skill that it does not separate the essence of the corresponding technical solution
The range of art scheme.
Claims (10)
1. a kind of sugarcane test tube seedling seedling nutritious soil, which is characterized in that the Nutrition Soil includes weathering pond sludge, river sand and phosphate fertilizer,
Wherein, the quality proportioning of the weathering pond sludge and river sand is 1:(0.8-1.2), and phosphorus content is 0.23%- in the Nutrition Soil
0.33%.
2. Nutrition Soil according to claim 1, which is characterized in that in the Nutrition Soil nitrogen content be 0.35%-0.45%,
Potassium content is 0.75%-0.95%；
Preferably, the PH of the Nutrition Soil is 5.5-6.5, specific gravity 2.60-2.70.
3. Nutrition Soil according to claim 1 or 2, which is characterized in that the weathering pond sludge be by fresh pond sludge from
So be exposed to the sun 3 months it is derived above；
Preferably, the water content of the weathering pond sludge is 8%-15%.
4. Nutrition Soil according to claim 1 to 3, which is characterized in that the weight proportion of the weathering pond sludge and river sand
It is 1: 1；
Preferably, the phosphorus content is 0.28%.
5. Nutrition Soil according to claim 1 to 4, which is characterized in that the weathering pond sludge partial size is 0.02-3mm.
6. -5 any Nutrition Soil according to claim 1, which is characterized in that the river sand is fresh river sand；
Preferably, the partial size of the river sand is 0.02-2mm.
7. -6 any Nutrition Soil according to claim 1, which is characterized in that the phosphate fertilizer is calcium superphosphate and/or calcium and magnesium
Phosphate fertilizer.
8. the preparation method of Nutrition Soil as claimed in claim 1 to 7, which comprises the steps of:
(1) weathering is carried out to fresh pond sludge, obtains weathering sugar mud；
(2) weathering pond sludge and river sand are mixed to get mixture；
(3) phosphate fertilizer is added in mixture, is uniformly mixed so as to obtain the Nutrition Soil；
It preferably, further include being crushed to weathering pond sludge in the step (1).
9. preparation method according to claim 8, which is characterized in that the weathering is that fresh pond sludge progress nature is sudden and violent
It shines；The time of being exposed to the sun is 90 days or more；It is described to be crushed to 0.02-3mm；
Preferably, the time of being exposed to the sun is 90-150 days；It is described to be crushed to 0.02-1mm.
10. a kind of sugarcane test tube seedling method for culturing seedlings, which is characterized in that carried out using Nutrition Soil as claimed in claim 1 to 7
Culture；
Preferably, the method for culturing seedlings includes the following steps:
(1) shoot proliferation cultivation cycle will be completed, and the unrooted sugarcane seedling bottle containing subculture multiplication medium is put in shady and cool, wind sheltering
Place, sprays foliage-spray liquid, then, carries out hardening treatment；
(2) test tube seedling after taking out hardening in bottle, is cleaned and is removed old leaf；
(3) test tube seedling after cleaning is carried out disinfection；
(4) test tube seedling after disinfection is planted in the planting pot for being placed with the Nutrition Soil, is watered with root water；
(5) planting pot is placed in seedling cultivation greenhouse, and covers sunshade net, until 90% test tube seedling grows young leaves, open sunshade net,
Seedling cultivation greenhouse environment is adjusted, and is spaced and carries out water drenching processing, until test tube seedling is out of the garden.
Preferably, the page spray liquid be IBA20mg/L, NAA10mg/L, sucrose 20g/L, L-Pro 100mg/L, native bacterium
Disappear 400mg/L, tween 2ml/L；
Preferably, the hardening treatment is, at 20-30 DEG C of temperature, humidity 65%-80%, intensity of illumination 2000-10000lux
Under, hardening 24-26h；
Preferably, the disinfection is 8-10min in the bright shield solution for immerse test tube seedling 500 times；
Preferably, the air humidity of the seedling rearing room is 40%-90%, intensity of illumination 2000-10000lux；
Preferably, adjusting nursery room environmental is that air humidity is adjusted to 68%-72%；
Preferably, the interval carries out water drenching processing to carry out water drenching 28-32s at interval of 25-35min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113545268A (en) * | 2021-08-13 | 2021-10-26 | 广西壮族自治区农业科学院 | Method for cultivating raw sugarcane by applying tissue culture single-plant temporary-planted seedlings of sugarcane |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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