CN107159691A - A kind of method of biological synergetic repairing heavy metal polluted soil of farmland - Google Patents

A kind of method of biological synergetic repairing heavy metal polluted soil of farmland Download PDF

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CN107159691A
CN107159691A CN201710424403.2A CN201710424403A CN107159691A CN 107159691 A CN107159691 A CN 107159691A CN 201710424403 A CN201710424403 A CN 201710424403A CN 107159691 A CN107159691 A CN 107159691A
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farmland
plant
heavy metal
soil
sprinkle
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李小兰
林晨
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Changzhou Xiang Xiang Technology Co Ltd
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
    • B09C1/00Reclamation of contaminated soil
    • B09C1/08Reclamation of contaminated soil chemically
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09CRECLAMATION OF CONTAMINATED SOIL
    • B09C1/00Reclamation of contaminated soil
    • B09C1/10Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • B09C1/105Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants

Abstract

The present invention relates to a kind of method of biological synergetic repairing heavy metal polluted soil of farmland, belong to technical field of soil remediation.Inductivity first of the invention filters out the plant that can be survived in high heavy metal concentration soil, the composition with chelated metal ions ability is extracted from Chinese medicine again, in Fructus Vitis viniferae fermented product under the auxiliary of organic carboxyl acid, further improve the complex performance of extract, obtain metal activation agent, with fermentation residue and Chinese medicine residue nutrient is provided for plant strain growth, and the heavy metal ion in soil is formed soluble chelate using preferred plant secretion and metal activation agent synergy, improve the enriching quantity of preferred plant root heavy metal, the abundant root system of preferred plant in soil is taken food finally by dispensing vole, the reparation to heavy metal pollution of soil can be completed after driving away vole by pouring water, the present invention passes through the synergy between different biologies, improve the enriching quantity of root system of plant heavy metal, and repairing efficiency is short, have broad application prospects.

Description

A kind of method of biological synergetic repairing heavy metal polluted soil of farmland
Technical field
The present invention relates to a kind of method of biological synergetic repairing heavy metal polluted soil of farmland, belong to soil restoring technology neck Domain.
Background technology
With a large amount of exploitation of mineral resources, industrial fast development and various chemical products, agricultural chemicals and change Fertile widely uses, and the pollutant containing heavy metal enters environment by all means, causes soil, especially an agricultural land soil huge sum of money Category pollution is increasingly serious.Heavy metal contaminants can not by chemistry or it is biodegradable, easily by food chain approach in plant, animal and People's cylinder accumulation, toxicity are big, and ecological environment, food security and health are constituted a serious threat.Therefore, an agricultural land soil huge sum of money Oneself turns into current increasingly serious environmental problem for category pollution, and its pollution source and recovery technique are also always the heat studied both at home and abroad Point and difficult point.
At present, heavy metal pollution of soil governance way mainly has two kinds, and one is the presence shape for changing heavy metal in soil State, makes it switch to stable state by activated state;Two be that heavy metal is removed from soil.Method mainly has gongwu chemical method, bag Include:Heavy metal pollution of soil, flushing complexometry, electronic chemical method, heat treating process etc., but be not suitable for the larger soil of contaminated area;It is biological Repairing method includes:The effect of phytoremediation, microorganism remediation and animal reparation etc., plant and lower animal repairing method to soil remediation Fruit is less notable;Microorganism remediation has absorption, precipitation, oxidation and reduction etc. using some of soil microorganism heavy metal Effect, so as to reduce the toxicity of heavy metal in soil.The technology can be such that heavy metal contaminants are removed from soil, to ring around Border influence is smaller, will not produce secondary pollution, the huge soil remediation potentiality having.But existing heavy-metal contaminated soil is micro- Bioremediation technology has some limitations, such as microorganism remediation duration length, slowly effect, generally form complex compound reduction The harm of heavy metal, can not remove heavy metal from soil.Phytoremediation technology is to utilize plant and its rhizosphere microorganism To the absorption of soil pollutant, volatilization, conversion, degraded, fixation and the recovery technique for removing pollutant in soil.At present It is found that many plants can have powerful accumulation ability to various heavy simultaneously, but most plants restorative procedure effect does not show Work, slowly effect, repair time are long, and repairing effect is unstable, are not suitable for the improvement of large area soil, along with by weather, The influence of soil environment so that the Accumulation of heavy metals amount of these plants is limited, so as to be above restricted in its application.
So, it would be highly desirable to it is small to find a kind of recovery project amount, will not destroy soil structures, repairing effect is good, is difficult by the external world The heavy metal restorative procedure of the influence of environment.
The content of the invention
Present invention mainly solves technical problem, in common remediation of heavy metal-contaminated soil at present, physics Chemical method quantities is big, investment is high, and destructible soil structures cause soil fertility to decline, cause secondary pollution, and biological prosthetic Method repairing effect is poor, and heavy metal accumulation amount is limited, easy climate, the defect of soil environment influence.There is provided a kind of biological synergetic The method of repairing heavy metal polluted soil of farmland.
In order to solve the above technical problems, the present invention is using technical scheme as described below:
(1)By waiting mass ratio to weigh lyme grass, paniculate rockcress seed, rape and Commeline communis seed, indoor seedling is put into after being soaked with warm water Nursery in bed, treats that seedling grows 2 true leaves, seedling is taken from seedbed, washes off root system soil with deionized water, then by children Seedling is transplanted in preferred basin;
(2)Daily to transplanting into seedling sprinkle 5~10mL nutrient solutions in preferred basin, quiescent culture stops drenching after 20~25 days Spill nutrient solution and be changed to sprinkle mass concentration for 200mg/L acetic acid lead solutions, it is 5~10mL/ days to control sprinkle amount, so cultivates 15 After it, stop sprinkle acetic acid lead solution and be changed to sprinkle mass concentration for 200mg/L copper nitrate solutions, control sprinkle amount be 5~ 10mL/ days, it was 200mg/L zinc acetate solutions to continue to stop sprinkle copper nitrate solution after cultivating 15 days to be changed to sprinkle mass concentration, It is 5~10mL/ days to control sprinkle amount, quiescent culture 15 days, after the completion of culture, and the plant under survival is preferred plant, standby;
(3)Count by weight, weigh 20~30 parts of scutellarias, 10~15 parts of rattletops, 5~10 parts of cordate houttuynias, 20~30 parts of cynomorium songaricums It is put into 30~40 parts of semen astragali complanatis in stone mortar, is ground and smashed to pieces repeatedly with stone pestle, obtains mixing Chinese medicine crushed material;
(4)Boiled after above-mentioned mixing Chinese medicine crushed material and water are mixed, be separated by filtration after the completion of boiling, respectively obtain Chinese medicine filter residue With middle drug filtrate, it is fitted into after grape and middle drug filtrate are mixed in fermentation tank, sealing and fermenting;
(5)It is separated by filtration after after above-mentioned fermentation ends, taking out tunning, respectively obtains fermentation filter residue and ferment filtrate, will send out Ferment filter residue and above-mentioned Chinese medicine filter residue are mixed to get hearth layer, and gained ferment filtrate is self-control metal activation agent, standby;
(6)Plantation hole is excavated in farmland to be repaired, above-mentioned hearth layer is added into each plantation hole, then above hearth layer One layer of soil is covered, finally by step(2)Standby preferred plant is planted in plantation hole, and preferred plant can be completed by returning native fixation Transplanting;
(7)Treat after the completion of above-mentioned preferred plant transplanting, the diversion into farmland to be repaired makes preferred plant grow 15~20 naturally My god, then by step(5)Standby self-control metal activation agent sprinkle makes preferred plant continue nature growth 15 into farmland to be repaired ~20 days;
(8)After it is above-mentioned naturally growth terminate after, add vole into farmland to be repaired, vole is taken food naturally in farmland to be repaired It is preferred that the coring of plant, was processed as after 10~15 days, the preferred plant removed in farmland to be repaired is gathered in harvester;
(9)After above-mentioned preferred plant harvesting removal terminates, it is 100m that irrigation volume is pressed into farmland3/ mu pours into water, drives away and catches Vole in farmland, after standing 3~5 days naturally, you can complete the reparation of heavy metal contaminated soil.
It by sand with concentration is that 2% salpeter solution and deionized water are rinsed 3~5 times respectively that described preferred basin, which is, then is pressed The amount of 500~600g/ basins, which is fitted into flowerpot, to be made.
Step(1)Described in warm water temperature be 20~35 DEG C, soak time be 6~8h, nursery temperature be 25~35 ℃。
Described nutrient solution is by 0.90~0.95 part of calcium nitrate, 0.50~0.52 part of potassium nitrate, 0.30~0.40 part of nitre Sour ammonium, 0.13~0.15 part of potassium dihydrogen phosphate, 0.40~0.50 part of magnesium sulfate, 0.01~0.03 part of ferrous sulfate and 0.05~ 0.08 part of sodium molybdate and 1000 parts of water mixed preparings are obtained.
Step(4)Described in mixing Chinese medicine crushed material and water mass ratio be 1:10, temperature is boiled for 100~110 DEG C, The time is boiled for 3~4h, the mass ratio of grape and middle drug filtrate is 1:5, sealing and fermenting temperature is 30~40 DEG C, during sealing and fermenting Between be 10~12 days.
Step(6)Described in plantation hole distance between rows and hills be 50cm × 40cm, plantation hole depth is 8~10cm, and hearth layer adds Add thickness as 1~2cm, the thickness of soil that hearth layer top is covered is 1cm.
Step(7)Described in diversion irrigation amount be 50m3/ mu, self-control metal activation agent sprinkle amount is 900~1000mL/ m2
Step(8)Described in vole add density for 90~100/mu.
The beneficial effects of the invention are as follows:
The present invention to several plants for being capable of enriching heavy metal first by carrying out inductivity screening and culturing, and choosing can be in a high huge sum of money The plant survived in category concentration soil, plants in farmland to be repaired, then extracted from Chinese medicine with chelated metal ions ability Composition, by extract and grape blending ferment, in fermentate under the auxiliary of organic carboxyl acid, further improve extract network Performance is closed, metal activation agent is obtained, nutrient is provided for plant strain growth with fermentation residue and Chinese medicine residue, and variety classes is preferred Plant enhances the secretion of plant root material in the competitive relation of same piece of land, and secretion and metal activation agent synergy make Heavy metal ion in soil forms the chelate of solubility, reduces soil pH value, strengthens the biological effectiveness of heavy metal, so that Improve the enriching quantity of preferred plant root heavy metal, after the completion of plant is enriched with again by deliver vole take food it is excellent in soil The abundant root system of plant is selected, the reparation to heavy metal pollution of soil can be completed after expeling vole of pouring water, the present invention passes through difference Synergy between biology, improves the enriching quantity of root system of plant heavy metal, and repairing efficiency is short, and heavy metal is gone Except excellent, have broad application prospects.
Embodiment
Weigh lyme grass, paniculate rockcress seed, rape and each 30~40g of Commeline communis seed, with 20~35 DEG C of warm water immersion 6~ The nursery at 25~35 DEG C in indoor seedbed is put into after 8h, treats that seedling grows 2 true leaves, seedling is taken from seedbed, spend from Sub- water washes off root system soil, then by sprigging in preferred basin;Daily to transplant into the seedling sprinkle 5 in preferred basin~ 10mL nutrient solutions, quiescent culture is after 20~25 days, and it is that 200mg/L lead acetates are molten to stop sprinkle nutrient solution to be changed to sprinkle mass concentration Liquid, it is 5~10mL/ days to control sprinkle amount, after so cultivating 15 days, and stopping sprinkle acetic acid lead solution is changed to sprinkle mass concentration and is 200mg/L copper nitrate solutions, it is 5~10mL/ days to control sprinkle amount, continues stopping sprinkle copper nitrate solution after cultivating 15 days and is changed to Sprinkle mass concentration is 200mg/L zinc acetate solutions, and it is 5~10mL/ days to control sprinkle amount, and quiescent culture 15 days, culture is completed Afterwards, the plant under surviving is preferred plant;Count by weight, weigh 20~30 parts of scutellarias, 10~15 parts of rattletops, 5~10 Part cordate houttuynia, 20~30 parts of cynomorium songaricums and 30~40 parts of semen astragali complanatis are put into stone mortar, are ground repeatedly with stone pestle and are smashed 30~40min to pieces and obtain To mixing Chinese medicine crushed material;It is 1 in mass ratio that Chinese medicine crushed material and water, which will be mixed,:After 10 mixing 3 are boiled at 100~110 DEG C ~4h, is separated by filtration after the completion of boiling, and respectively obtains Chinese medicine filter residue and middle drug filtrate, then is 1 in mass ratio:5 by grape and in Drug filtrate mixing after is fitted into fermentation tank, seal tank mouth after at 30~40 DEG C sealing and fermenting 10~12 days;After after fermentation ends, Take out tunning to be separated by filtration, respectively obtain fermentation filter residue and ferment filtrate, fermentation filter residue and Chinese medicine filter residue are mixed to get Hearth layer, gained ferment filtrate is self-control metal activation agent;It is that 50cm × 40cm is opened in farmland to be repaired by distance between rows and hills Plantation hole is dug, it is 8~10cm to control plantation hole depth, the hearth layer that addition thickness is 1~2cm into each plantation hole, then The soil that covering a layer thickness is 1cm above hearth layer, finally plants preferred plant in plantation hole, and returning native fixation can complete It is preferred that plant is transplanted;Treat after the completion of preferred plant transplanting, be 50m by irrigation volume3The diversion into farmland to be repaired of/mu, makes preferably Plant is grown 15~20 days naturally, then the agent of self-control metal activation is pressed into sprinkle amount for 900~1000mL/m2Sprinkle is to agriculture to be repaired Tanaka, makes preferred plant continue nature and grows 15~20 days;It is 90~100/mu by density is added after growth terminates naturally Vole is added into farmland to be repaired, vole is taken food the coring of preferred plant in farmland to be repaired naturally, it is processed as 10~ After 15 days, the preferred plant removed in farmland to be repaired is gathered in harvester;After the harvesting removal of preferred plant terminates, to farmland In by irrigation volume be 100m3/ mu pours into water, drives away the vole caught in farmland, after standing 3~5 days naturally, you can complete counterweight The reparation of metallic pollution soil.It by sand with concentration is that 2% salpeter solution and deionized water rinse 3 respectively that described preferred basin, which is, ~5 times, then it is fitted into flowerpot obtained by the amount of 500~600g/ basins.Described nutrient solution be by 0.90~0.95 part of calcium nitrate, 0.50~0.52 part of potassium nitrate, 0.30~0.40 part of ammonium nitrate, 0.13~0.15 part of potassium dihydrogen phosphate, 0.40~0.50 part of sulfuric acid Magnesium, 0.01~0.03 part of ferrous sulfate and 0.05~0.08 part of sodium molybdate and 1000 parts of water mixed preparings are obtained.
Example 1
Lyme grass, paniculate rockcress seed, rape and each 40g of Commeline communis seed are weighed, indoor seedling is put into after soaking 8h with 35 DEG C of warm water The nursery at 35 DEG C in bed, treats that seedling grows 2 true leaves, seedling is taken from seedbed, root system mud is washed off with deionized water Soil, then by sprigging in preferred basin;Daily to transplanting into the seedling sprinkle 10mL nutrient solutions in preferred basin, quiescent culture 25 After it, stop sprinkle nutrient solution and be changed to sprinkle mass concentration for 200mg/L acetic acid lead solutions, it is 10mL/ days to control sprinkle amount, such as After this culture 15 days, stop sprinkle acetic acid lead solution and be changed to sprinkle mass concentration for 200mg/L copper nitrate solutions, control sprinkle amount For 10mL/ days, it was that 200mg/L zinc acetates are molten to continue to stop sprinkle copper nitrate solution after cultivating 15 days to be changed to sprinkle mass concentration Liquid, it is 10mL/ days to control sprinkle amount, quiescent culture 15 days, after the completion of culture, and the plant under survival is preferred plant;By weight Number meter is measured, 30 parts of scutellarias, 15 parts of rattletops, 10 portions of cordate houttuynias, 30 parts of cynomorium songaricums and 40 parts of semen astragali complanatis is weighed and is put into stone mortar, use stone Pestle grind repeatedly smash to pieces 40min obtain mix Chinese medicine crushed material;It is 1 in mass ratio that Chinese medicine crushed material and water, which will be mixed,:10 mixing 4h is boiled at 110 DEG C afterwards, is separated by filtration after the completion of boiling, Chinese medicine filter residue and middle drug filtrate is respectively obtained, then be in mass ratio 1:5 will grape and middle drug filtrate mix after is fitted into fermentation tank, seal after tank mouth sealing and fermenting 12 days at 40 DEG C;Treat fermentation knot Shu Hou, takes out tunning and is separated by filtration, respectively obtain fermentation filter residue and ferment filtrate, and fermentation filter residue and Chinese medicine filter residue are mixed Hearth layer is obtained, gained ferment filtrate is self-control metal activation agent;By distance between rows and hills be 50cm × 40cm in farmland to be repaired Middle excavation plantation hole, it is 10cm to control plantation hole depth, and the hearth layer that thickness is 2cm is added into each plantation hole, then in pad The soil that covering a layer thickness is 1cm above bed material, finally plants preferred plant in plantation hole, and returning native fixation can complete excellent Plant is selected to transplant;Treat after the completion of preferred plant transplanting, be 50m by irrigation volume3The diversion into farmland to be repaired of/mu, makes preferred plant Strain grows 20 days naturally, then it is 1000mL/m that will make metal activation agent by oneself by sprinkle amount2Sprinkle makes preferably into farmland to be repaired Plant continues nature and grown 20 days;After growth terminates naturally, added by density is added for 100/mu into farmland to be repaired Vole, makes vole take food the coring of preferred plant in farmland to be repaired naturally, is processed as after 15 days, is gathered in and removed with harvester Preferred plant in farmland to be repaired;After the harvesting removal of preferred plant terminates, it is 100m that irrigation volume is pressed into farmland3/ mu is filled Enter water, drive away the vole caught in farmland, after standing 5 days naturally, you can complete the reparation of heavy metal contaminated soil.Described It is preferred that it by sand with concentration is that 2% salpeter solution and deionized water are rinsed 5 times respectively that basin, which is, then load flowerpot by the amount of 600g/ basins In be made.Described nutrient solution is by 0.95 part of calcium nitrate, 0.52 part of potassium nitrate, 0.40 part of ammonium nitrate, 0.15 part of biphosphate Potassium, 0.50 part of magnesium sulfate, 0.03 part of ferrous sulfate and 0.08 part of sodium molybdate and 1000 parts of water mixed preparings are obtained.
Example 2
Lyme grass, paniculate rockcress seed, rape and each 30g of Commeline communis seed are weighed, indoor seedling is put into after soaking 6h with 20 DEG C of warm water The nursery at 25 DEG C in bed, treats that seedling grows 2 true leaves, seedling is taken from seedbed, root system mud is washed off with deionized water Soil, then by sprigging in preferred basin;Daily to transplanting into the seedling sprinkle 5mL nutrient solutions in preferred basin, quiescent culture 20 After it, stop sprinkle nutrient solution and be changed to sprinkle mass concentration for 200mg/L acetic acid lead solutions, it is 5mL/ days to control sprinkle amount, such as After this culture 15 days, stop sprinkle acetic acid lead solution and be changed to sprinkle mass concentration for 200mg/L copper nitrate solutions, control sprinkle amount For 5mL/ days, it was 200mg/L zinc acetate solutions to continue to stop sprinkle copper nitrate solution after cultivating 15 days to be changed to sprinkle mass concentration, It is 5mL/ days to control sprinkle amount, quiescent culture 15 days, after the completion of culture, and the plant under survival is preferred plant;By weight Number meter, weighs 20 parts of scutellarias, 10 parts of rattletops, 5 portions of cordate houttuynias, 20 parts of cynomorium songaricums and 30 parts of semen astragali complanatis and is put into stone mortar, anti-with stone pestle Multiple grinding smashs 30min to pieces and obtains mixing Chinese medicine crushed material;It is 1 in mass ratio that Chinese medicine crushed material and water, which will be mixed,:10 mixing after 3h is boiled at 100 DEG C, is separated by filtration after the completion of boiling, Chinese medicine filter residue and middle drug filtrate is respectively obtained, then is 1 in mass ratio:5 will Grape and middle drug filtrate mixing after is fitted into fermentation tank, seal tank mouth after at 30 DEG C sealing and fermenting 10 days;After after fermentation ends, Take out tunning to be separated by filtration, respectively obtain fermentation filter residue and ferment filtrate, fermentation filter residue and Chinese medicine filter residue are mixed to get Hearth layer, gained ferment filtrate is self-control metal activation agent;It is that 50cm × 40cm is opened in farmland to be repaired by distance between rows and hills Plantation hole is dug, it is 8cm to control plantation hole depth, the hearth layer that thickness is 1cm is added into each plantation hole, then on hearth layer The soil that side's covering a layer thickness is 1cm, finally plants preferred plant in plantation hole, and preferred plant can be completed by returning native fixation Transplanting;Treat after the completion of preferred plant transplanting, be 50m by irrigation volume3The diversion into farmland to be repaired of/mu, makes preferred plant natural Growth 15 days, then the agent of self-control metal activation is pressed into sprinkle amount for 900mL/m2Sprinkle into farmland to be repaired, make preferred plant after Continuous nature grows 15 days;After growth terminates naturally, it is that 90/mu add vole into farmland to be repaired by density is added, makes Vole takes food the coring of preferred plant in farmland to be repaired naturally, is processed as after 10 days, is removed with harvester harvesting to be repaired Preferred plant in farmland;After the harvesting removal of preferred plant terminates, it is 100m that irrigation volume is pressed into farmland3/ mu pours into water, drives Except the vole caught in farmland, after standing 3 days naturally, you can complete the reparation of heavy metal contaminated soil.Described preferred basin It by sand with concentration is that 2% salpeter solution and deionized water are rinsed 3 times respectively to be, then is fitted into system in flowerpot by the amount of 500g/ basins .Described nutrient solution be by 0.90 part of calcium nitrate, 0.50 part of potassium nitrate, 0.30 part of ammonium nitrate, 0.13 part of potassium dihydrogen phosphate, 0.40 part of magnesium sulfate, 0.01 part of ferrous sulfate and 0.05 part of sodium molybdate and 1000 parts of water mixed preparings are obtained.
Example 3
Lyme grass, paniculate rockcress seed, rape and each 35g of Commeline communis seed are weighed, indoor seedling is put into after soaking 7h with 30 DEG C of warm water The nursery at 32 DEG C in bed, treats that seedling grows 2 true leaves, seedling is taken from seedbed, root system mud is washed off with deionized water Soil, then by sprigging in preferred basin;Daily to transplanting into the seedling sprinkle 7mL nutrient solutions in preferred basin, quiescent culture 22 After it, stop sprinkle nutrient solution and be changed to sprinkle mass concentration for 200mg/L acetic acid lead solutions, it is 7mL/ days to control sprinkle amount, such as After this culture 15 days, stop sprinkle acetic acid lead solution and be changed to sprinkle mass concentration for 200mg/L copper nitrate solutions, control sprinkle amount For 7mL/ days, it was 200mg/L zinc acetate solutions to continue to stop sprinkle copper nitrate solution after cultivating 15 days to be changed to sprinkle mass concentration, It is 7mL/ days to control sprinkle amount, quiescent culture 15 days, after the completion of culture, and the plant under survival is preferred plant;By weight Number meter, weighs 25 parts of scutellarias, 12 parts of rattletops, 7 portions of cordate houttuynias, 25 parts of cynomorium songaricums and 35 parts of semen astragali complanatis and is put into stone mortar, anti-with stone pestle Multiple grinding smashs 35min to pieces and obtains mixing Chinese medicine crushed material;It is 1 in mass ratio that Chinese medicine crushed material and water, which will be mixed,:10 mixing after 4h is boiled at 105 DEG C, is separated by filtration after the completion of boiling, Chinese medicine filter residue and middle drug filtrate is respectively obtained, then is 1 in mass ratio:5 will Grape and middle drug filtrate mixing after is fitted into fermentation tank, seal tank mouth after at 35 DEG C sealing and fermenting 11 days;After after fermentation ends, Take out tunning to be separated by filtration, respectively obtain fermentation filter residue and ferment filtrate, fermentation filter residue and Chinese medicine filter residue are mixed to get Hearth layer, gained ferment filtrate is self-control metal activation agent;It is that 50cm × 40cm is opened in farmland to be repaired by distance between rows and hills Plantation hole is dug, it is 9cm to control plantation hole depth, the hearth layer that thickness is 1cm is added into each plantation hole, then on hearth layer The soil that side's covering a layer thickness is 1cm, finally plants preferred plant in plantation hole, and preferred plant can be completed by returning native fixation Transplanting;Treat after the completion of preferred plant transplanting, be 50m by irrigation volume3The diversion into farmland to be repaired of/mu, makes preferred plant natural Growth 17 days, then the agent of self-control metal activation is pressed into sprinkle amount for 950mL/m2Sprinkle into farmland to be repaired, make preferred plant after Continuous nature grows 17 days;After growth terminates naturally, it is that 95/mu add vole into farmland to be repaired by density is added, makes Vole takes food the coring of preferred plant in farmland to be repaired naturally, is processed as after 12 days, is removed with harvester harvesting to be repaired Preferred plant in farmland;After the harvesting removal of preferred plant terminates, it is 100m that irrigation volume is pressed into farmland3/ mu pours into water, drives Except the vole caught in farmland, after standing 4 days naturally, you can complete the reparation of heavy metal contaminated soil.Described preferred basin It by sand with concentration is that 2% salpeter solution and deionized water are rinsed 4 times respectively to be, then is fitted into system in flowerpot by the amount of 550g/ basins .Described nutrient solution be by 0.92 part of calcium nitrate, 0.51 part of potassium nitrate, 0.35 part of ammonium nitrate, 0.14 part of potassium dihydrogen phosphate, 0.45 part of magnesium sulfate, 0.02 part of ferrous sulfate and 0.07 part of sodium molybdate and 1000 parts of water mixed preparings are obtained.
Reference examples 1:Repaired using phytoremediation, the plant used is herbage.
Reference examples 2:Repaired using microbial method, the microorganism used is sulfatereducting bacteria.
Reference examples 3:Repaired using animal reparation method, the animal used is earthworm.
Content of beary metal heavy metal soil unanimous on the whole is chosen, is repaiied with the restorative procedure of example and reference examples It is multiple, and the soil in the farmland after example and reference examples reparation is sampled, the pH for detecting soil is 6.8~7.2, further according to 《Standard of soil environment quality》GB 15618-1995 standard carries out detection content of beary metal, detects data such as table 1.
Zinc(mg/kg) Lead(mg/kg) Copper(mg/kg) Cadmium(mg/kg)
Before untreated 856 5458 229 7.2
Example 1 210 200 32 0.15
Example 2 190 180 30 0.10
Example 3 195 220 29 0.13
Reference examples 1 260 270 65 1.5
Reference examples 2 230 252 45 0.8
Reference examples 3 240 260 56 1.2
As shown in Table 1, heavy metal restorative procedure repairing effect of the present invention is good, reaches farmland governance standard, is conducive to farming Thing grows.

Claims (8)

1. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland, it is characterised in that specifically repairing step is:
(1)By waiting mass ratio to weigh lyme grass, paniculate rockcress seed, rape and Commeline communis seed, indoor seedling is put into after being soaked with warm water Nursery in bed, treats that seedling grows 2 true leaves, seedling is taken from seedbed, washes off root system soil with deionized water, then by children Seedling is transplanted in preferred basin;
(2)Daily to transplanting into seedling sprinkle 5~10mL nutrient solutions in preferred basin, quiescent culture stops drenching after 20~25 days Spill nutrient solution and be changed to sprinkle mass concentration for 200mg/L acetic acid lead solutions, it is 5~10mL/ days to control sprinkle amount, so cultivates 15 After it, stop sprinkle acetic acid lead solution and be changed to sprinkle mass concentration for 200mg/L copper nitrate solutions, control sprinkle amount be 5~ 10mL/ days, it was 200mg/L zinc acetate solutions to continue to stop sprinkle copper nitrate solution after cultivating 15 days to be changed to sprinkle mass concentration, It is 5~10mL/ days to control sprinkle amount, quiescent culture 15 days, after the completion of culture, and the plant under survival is preferred plant, standby;
(3)Count by weight, weigh 20~30 parts of scutellarias, 10~15 parts of rattletops, 5~10 parts of cordate houttuynias, 20~30 parts of cynomorium songaricums It is put into 30~40 parts of semen astragali complanatis in stone mortar, is ground and smashed to pieces repeatedly with stone pestle, obtains mixing Chinese medicine crushed material;
(4)Boiled after above-mentioned mixing Chinese medicine crushed material and water are mixed, be separated by filtration after the completion of boiling, respectively obtain Chinese medicine filter residue With middle drug filtrate, it is fitted into after grape and middle drug filtrate are mixed in fermentation tank, sealing and fermenting;
(5)It is separated by filtration after after above-mentioned fermentation ends, taking out tunning, respectively obtains fermentation filter residue and ferment filtrate, will send out Ferment filter residue and above-mentioned Chinese medicine filter residue are mixed to get hearth layer, and gained ferment filtrate is self-control metal activation agent, standby;
(6)Plantation hole is excavated in farmland to be repaired, above-mentioned hearth layer is added into each plantation hole, then above hearth layer One layer of soil is covered, finally by step(2)Standby preferred plant is planted in plantation hole, and preferred plant can be completed by returning native fixation Transplanting;
(7)Treat after the completion of above-mentioned preferred plant transplanting, the diversion into farmland to be repaired makes preferred plant grow 15~20 naturally My god, then by step(5)Standby self-control metal activation agent sprinkle makes preferred plant continue nature growth 15 into farmland to be repaired ~20 days;
(8)After it is above-mentioned naturally growth terminate after, add vole into farmland to be repaired, vole is taken food naturally in farmland to be repaired It is preferred that the coring of plant, was processed as after 10~15 days, the preferred plant removed in farmland to be repaired is gathered in harvester;
(9)After above-mentioned preferred plant harvesting removal terminates, it is 100m that irrigation volume is pressed into farmland3/ mu pours into water, drives away and catches Vole in farmland, after standing 3~5 days naturally, you can complete the reparation of heavy metal contaminated soil.
2. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland according to claim 1, it is characterised in that: It by sand with concentration is that 2% salpeter solution and deionized water are rinsed 3~5 times respectively that described preferred basin, which is, then by 500~600g/ The amount of basin, which is fitted into flowerpot, to be made.
3. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland according to claim 1, it is characterised in that: Step(1)Described in warm water temperature be 20~35 DEG C, soak time be 6~8h, nursery temperature be 25~35 DEG C.
4. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland according to claim 1, it is characterised in that: Described nutrient solution is by 0.90~0.95 part of calcium nitrate, 0.50~0.52 part of potassium nitrate, 0.30~0.40 part of ammonium nitrate, 0.13 ~0.15 part of potassium dihydrogen phosphate, 0.40~0.50 part of magnesium sulfate, 0.01~0.03 part of ferrous sulfate and 0.05~0.08 part of molybdic acid Sodium and 1000 parts of water mixed preparings are obtained.
5. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland according to claim 1, it is characterised in that: Step(4)Described in mixing Chinese medicine crushed material and water mass ratio be 1:10, temperature is boiled for 100~110 DEG C, boils the time For 3~4h, the mass ratio of grape and middle drug filtrate is 1:5, sealing and fermenting temperature be 30~40 DEG C, the sealing and fermenting time be 10~ 12 days.
6. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland according to claim 1, it is characterised in that: Step(6)Described in plantation hole distance between rows and hills be 50cm × 40cm, plantation hole depth be 8~10cm, hearth layer addition thickness be 1 The thickness of soil of covering is 1cm above~2cm, hearth layer.
7. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland according to claim 1, it is characterised in that: Step(7)Described in diversion irrigation amount be 50m3/ mu, self-control metal activation agent sprinkle amount is 900~1000mL/m2
8. a kind of method of biological synergetic repairing heavy metal polluted soil of farmland according to claim 1, it is characterised in that: Step(8)Described in vole add density for 90~100/mu.
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107258152A (en) * 2017-07-04 2017-10-20 华能澜沧江水电股份有限公司 A kind of method of lyme grass seed quick-speed germination
CN111872108A (en) * 2020-06-18 2020-11-03 宋红刚 Plant root system growth induction device based on heavy metal soil restoration technology
CN112893449A (en) * 2021-01-08 2021-06-04 河南大学 Method for repairing heavy metal in tidal flat land based on animals and plants

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CN104550208A (en) * 2013-10-12 2015-04-29 中环循(北京)环境技术中心 Farmland soil heavy metal pollution restoration method through coupling activating and passivating
CN105170618A (en) * 2015-09-30 2015-12-23 河南行知专利服务有限公司 Method for remediating heavy metal contaminated soil through cooperation of activating agents and plants

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Publication number Priority date Publication date Assignee Title
CN104550208A (en) * 2013-10-12 2015-04-29 中环循(北京)环境技术中心 Farmland soil heavy metal pollution restoration method through coupling activating and passivating
CN105170618A (en) * 2015-09-30 2015-12-23 河南行知专利服务有限公司 Method for remediating heavy metal contaminated soil through cooperation of activating agents and plants

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107258152A (en) * 2017-07-04 2017-10-20 华能澜沧江水电股份有限公司 A kind of method of lyme grass seed quick-speed germination
CN111872108A (en) * 2020-06-18 2020-11-03 宋红刚 Plant root system growth induction device based on heavy metal soil restoration technology
CN112893449A (en) * 2021-01-08 2021-06-04 河南大学 Method for repairing heavy metal in tidal flat land based on animals and plants

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