CN115474459B - Method for promoting germination of polygonatum sibiricum seeds - Google Patents

Method for promoting germination of polygonatum sibiricum seeds Download PDF

Info

Publication number
CN115474459B
CN115474459B CN202211236885.6A CN202211236885A CN115474459B CN 115474459 B CN115474459 B CN 115474459B CN 202211236885 A CN202211236885 A CN 202211236885A CN 115474459 B CN115474459 B CN 115474459B
Authority
CN
China
Prior art keywords
seeds
primary
soaking
solution
rhizome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202211236885.6A
Other languages
Chinese (zh)
Other versions
CN115474459A (en
Inventor
余国梁
杨华
郭纯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hunan Agricultural University
Original Assignee
Hunan Agricultural University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hunan Agricultural University filed Critical Hunan Agricultural University
Priority to CN202211236885.6A priority Critical patent/CN115474459B/en
Publication of CN115474459A publication Critical patent/CN115474459A/en
Application granted granted Critical
Publication of CN115474459B publication Critical patent/CN115474459B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00—Plant growth regulators

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Soil Sciences (AREA)
  • Toxicology (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physiology (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a method for promoting germination of polygonatum sibiricum seeds, which comprises the following steps: (1) Soaking rhizoma Polygonati seeds in gibberellin solution for a set time, transferring to matrix culture, and removing dormancy once to obtain primary rhizome; (2) Soaking the primary rhizome in a growth regulator solution for a set time, refrigerating the primary rhizome, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedling. The method for promoting seed germination has good effect in breaking the dormancy breaking of the polygonatum sibiricum seeds, the occurrence rate of the primary rootstock of the seeds and the emergence rate of the primary rootstock are both remarkably improved, the success rate of breaking the primary dormancy is more than 80%, and the success rate of breaking the secondary dormancy is more than 90%; meanwhile, the emergence time of the polygonatum seeds is greatly shortened, and the time cost of polygonatum breeding and seedling raising is reduced.

Description

Method for promoting germination of polygonatum sibiricum seeds
Technical Field
The invention relates to the technical field of crop breeding and seedling raising, in particular to a method for promoting germination of polygonatum sibiricum seeds.
Background
Dormancy of Polygonatum sibiricum seed belongs to comprehensive dormancy. The seed coat and endosperm structure slows down the water uptake of the seed; after the seeds are ripe, the seeds enter a dormant state through a physiological dormant process; the germination inhibiting substance contained in the seed affects the germination of the seed. The water absorption and analysis experiments show that the structures of the seed coats and endosperm limit the penetration of water; the content of the polygonatum embryo is little, the polygonatum embryo is not mature in physiology, the germination requirement is not met, and a long time is needed to provide a material basis for germination. By adopting fermentation and temperature changing, the lamination treatment can break dormancy and promote the after-ripening process of the embryo. The conventional breeding process of rhizoma polygonati generally adopts the following method: picking mature fruits of Polygonatum cyrtonema Fabricius at the bottom of 10 months in the same year, rubbing to obtain clean seeds, sand-storing for 1 winter, taking out the seeds in the middle of 3 months in the second year, sowing the seeds in a flowerpot with moist and fertile matrixes, and emergence in spring in the third year; the whole process generally takes 12 months from sowing seeds to growing seedlings, and the period is long. The prior literature discloses a technical scheme (Zhu Wufeng, research on the breeding technology of rhizoma polygonati of medicinal plants [ D ], university of agriculture and forestry in North China, 2013) for promoting the germination of rhizoma polygonati seeds by soaking the seeds in clear water at the temperature of 25-50 ℃, but the technical problem of low emergence rate exists in the practical application process although the shortening effect on the breeding and seedling time to a certain extent can be generated.
Disclosure of Invention
The invention provides a method for promoting germination of polygonatum sibiricum seeds, which is used for solving the technical problems of overlong emergence period and low emergence rate of the existing polygonatum sibiricum breeding and seedling raising technology.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for promoting germination of polygonatum sibiricum seeds, comprising the steps of:
(1) Soaking the Polygonatum sibiricum seed in gibberellin solution for a set time, transferring to a substrate for culturing, and removing one dormancy to obtain primary rootstocks;
(2) And (3) soaking the primary rootstock in a growth regulator solution for a set time, refrigerating the primary rootstock, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the rhizoma polygonati seedling.
The design concept of the technical scheme is that gibberellin is an important plant growth hormone, belongs to tetracyclic diterpenoid compounds, and is studied for breaking seed dormancy through gibberellin treatment in the prior art, but the inventor finds that compared with other plants, the germination of polygonatum sibiricum seeds is more obviously affected by gibberellin, the invention treats polygonatum sibiricum seeds through gibberellin solution, breaks primary dormancy of polygonatum sibiricum seeds, improves water permeability and air permeability of seeds, enhances respiration of seeds, promotes expansion of embryo, promotes synthesis of auxin in the seeds, further promotes division elongation of embryo cells, improves physiological activity of germination promoting enzymes such as amylase and the like, thereby obviously improving germination rate of polygonatum sibiricum seeds, and on the other hand, breaks secondary dormancy of polygonatum sibiricum seeds through a plant growth regulator, so that when the germination rate is greatly improved, the germination time is shortened, the time cost of polygonatum sibiricum seeds is saved, the primary rootstock rate of polygonatum sibiricum seeds reaches more than 70%, the germination rate of the primary rootstock seeds is improved to 90%, the germination rate of the polygonatum sibiricum seeds is greatly improved, the seedling raising efficiency is greatly improved, and the economic effect on crops is obvious.
The soaking concentration, the treatment temperature and the treatment time of the hormone are significant for promoting seed germination, the low-concentration hormone can hardly activate related signal paths, and the high-concentration hormone can inhibit related growth and development processes; proper soaking temperature can create a proper environment for starting germination of seeds, the enzyme activity of the seeds is not high at low temperature, and related enzymes of the seeds can be deactivated at high temperature, so that the seeds cannot germinate; the proper soaking time is also vice versa, the soaking time is too short to achieve the effect, and the soaking time is too long, so that seeds can be choked or oxygen-free respiration can be started, seeds are poisoned, and the germination rate is reduced.
As a further preferable aspect of the above technical scheme, the gibberellin solution is GA 1 Is a solution of (a) and (b). Gibberellins are of a wide variety, and there are 136 gibberellins found at present, collectively referred to as gibberellins (GA S ) The prior art generally adopts GA 3 The seeds were treated to promote germination, but during actual cultivation the inventors found that GA 3 Because the activity is too high, the higher seed mildew rate can be caused, the germination rate of the seeds is affected, and the overgrowth of hypocotyls can be promoted when the dormancy of plants is broken, so that the lodging resistance of the plants is reduced; based on the studies, the inventors found that GA 1 The method is more suitable for the treatment of the polygonatum sibiricum seeds, can smoothly break the dormancy of the seeds and can not cause the growth of hypocotyls, and can reduce the mildew rate of the seeds while avoiding the occurrence of plant lodging phenomenon, thereby improving the germination rate of the seeds.
As a further preferable aspect of the above technical scheme, the concentration of the gibberellin solution is 30-50 mg/L. The concentration of the gibberellin solution is one of the key parameters affecting the occurrence rate of primary rhizomes of seeds, and the sealwort seeds have the optimal occurrence rate of primary rhizomes after being treated by the gibberellin solution within the concentration range.
As a further preferable mode of the technical scheme, the soaking time of the polygonatum sibiricum seeds in the step (1) in the gibberellin solution is 24-72 hours, and the soaking temperature is 30-40 ℃. After soaking treatment within the above time and temperature ranges, the rhizoma polygonati seeds have the optimal occurrence rate of primary rootstocks.
As a further preferred aspect of the above-mentioned technical scheme, the growth regulator solution is a 6- (2-hydroxybenzylamino) purine solution.
As a further preferable aspect of the above technical scheme, the concentration of the growth regulator solution is 200-400 mg/L.
As a further preferable mode of the technical scheme, the soaking time of the polygonatum sibiricum seeds in the growth regulator solution in the step (2) is 12-24 hours.
As a further preferable mode of the above technical scheme, the refrigerating temperature of the nascent rhizome in the step (2) is 4 ℃ and the refrigerating time is 30-60 days.
As a further preferable mode of the technical scheme, the method for obtaining the polygonatum seeds comprises the steps of picking the polygonatum fruits which are not fully mature, removing impurities, spreading and airing volatile part of water for two days, sealing at 4 ℃, fermenting at a low temperature for 90 days, taking out, rubbing seed coats in clear water, and collecting seeds which are sunk under water.
Compared with the prior art, the invention has the advantages that:
the method for promoting seed germination has good effect in breaking the dormancy breaking of the polygonatum sibiricum seeds, the occurrence rate of the primary rootstock of the seeds and the emergence rate of the primary rootstock are both remarkably improved, the success rate of breaking the primary dormancy is more than 80%, and the success rate of breaking the secondary dormancy is more than 90%; meanwhile, the emergence time of the polygonatum seeds is greatly shortened, and the time cost of polygonatum breeding and seedling raising is reduced.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1:
the method for promoting germination of rhizoma polygonati seeds of the embodiment comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the method for measuring the vigor of the polygonatum sibiricum seeds comprises two steps, namely, firstly, a TTC method is adopted to test the vigor of the seeds: soaking seeds in water bath at 30 ℃ for 6 hours, and dyeing with TTC solution with concentration of 0.3% for 24 hours in dark place. Placing the seeds in a culture dish, exposing the obvious embryo structure as much as possible when cutting the seeds, and simultaneously boiling the seeds with boiling water for contrast treatment, wherein the embryo is dyed red and is a living seed; and then testing the vitality of the seeds by adopting a red ink method: taking half of the seeds remained by the TTC method, placing the seeds into a culture dish, adding 5% of red ink for dyeing for 20 minutes, pouring out the red ink, then flushing the seeds with tap water for multiple times, and observing the dyeing condition. The seed embryo is not colored or is lightly colored, and the seed embryo and endosperm are dead seeds with the same degree of coloring. Taking the average value of the results of the two detection methods as the final measurement result of the seed vitality;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 1 Soaking in the solution for 72h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃; through testing, the occurrence rate of primary rhizomes of the polygonatum sibiricum seeds treated in the step (2) is 77.3%, and the mildew rate of the seeds is 5%;
(3) Soaking the primary rhizome in a 6- (2-hydroxybenzylamino) purine solution with the concentration of 400mg/L for 24 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedlings; the germination rate of the nascent rootstocks treated in the step (3) is 90 percent through testing.
Example 2:
the method for promoting germination of rhizoma polygonati seeds of the embodiment comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA with concentration of 40mg/L 1 Soaking in the solution for 24h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 30 ℃; through testing, the occurrence rate of primary rhizomes of the polygonatum sibiricum seeds treated in the step (2) is 68%, and the mildew rate of the seeds is 8.7%;
(3) Soaking the primary rhizome in a solution of 6- (2-hydroxybenzylamino) purine with the concentration of 300mg/L for 12 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedlings; the germination rate of the nascent rootstocks treated in the step (3) is 81.9 percent through test.
Example 3:
the method for promoting germination of rhizoma polygonati seeds of the embodiment comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 30mg/L 1 Soaking in the solution for 24h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 30 ℃; through testing, the occurrence rate of primary rhizomes of the polygonatum sibiricum seeds treated in the step (2) is 67.3%, and the mildew rate of the seeds is 9.3%;
(3) Soaking the primary rhizome in a solution of 6- (2-hydroxybenzylamino) purine with the concentration of 300mg/L for 12 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedlings; the germination rate of the nascent rootstocks treated in the step (3) is 82.3 percent through test.
Example 4:
the method for promoting germination of rhizoma polygonati seeds of the embodiment comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 1 Soaking in the solution for 48h, transferring to a matrix for culturing, and removing one dormancy to obtain primary rhizome, wherein the soaking temperature is 35 ℃; through testing, the occurrence rate of primary rhizomes of the polygonatum sibiricum seeds treated in the step (2) is 72.7%, and the mildew rate of the seeds is 7.3%;
(3) Soaking the primary rhizome in a solution of 6- (2-hydroxybenzylamino) purine with the concentration of 300mg/L for 12 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedlings; the germination rate of the nascent rootstocks treated in the step (3) is 80.3 percent through test.
Example 5:
the method for promoting germination of rhizoma polygonati seeds of the embodiment comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 1 Soaking in the solution for 72h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃; through testing, the occurrence rate of primary rhizomes of the polygonatum sibiricum seeds treated in the step (2) is equal to the mildewing rate of the seeds;
(3) Soaking the primary rhizome in a 6- (2-hydroxybenzylamino) purine solution with the concentration of 200mg/L for 24 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedlings; the germination rate of the nascent rootstocks treated in the step (3) is 63% through testing.
Example 6:
the method for promoting germination of rhizoma polygonati seeds of the embodiment comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 1 Soaking in the solution for 72h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃; through testing, the occurrence rate of primary rhizomes of the polygonatum sibiricum seeds treated in the step (2) is equal to the mildewing rate of the seeds;
(3) Soaking the primary rhizome in a 6- (2-hydroxybenzylamino) purine solution with the concentration of 300mg/L for 12 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, breaking secondary dormancy, and testing to obtain the rhizoma polygonati seedling, wherein the germination rate of the primary rhizome treated in the step (3) is 83%.
Comparative example 1:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 3 Soaking in the solution for 48h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4deg.C for 30 days, transferring to substrate for cultivation, and breaking secondary dormancy to obtain rhizoma Polygonati seedling.
The condition of the primary rhizome of the polygonatum sibiricum seed treated in the step (2) is tested, and the incidence rate of the primary rhizome of the polygonatum sibiricum seed treated in the comparative example is only 10%, and the mildew rate of the seed is up to 22.5%.
The comparative example also adjusts the soaking temperature in the step (2), the soaking temperature is set to be 23 ℃, 26 ℃, 29 ℃ and 32 ℃, other parameters are unchanged, and the condition of the primary rhizome of the polygonatum sibiricum seed treated in the step (2) is tested, and the result is shown in the following table 1:
TABLE 1 gibberellin GA at different soaking temperatures 3 Influence on primary dormancy breaking of Polygonatum sibiricum seed
Culture temperature Germination percentage Mould generation rate
23℃ 3％ 17.5％
26℃ 10％ 22.5％
29℃ 0 32.5％
32℃ 0 66.7％
The comparative example also adjusts gibberellin GA in step (2) 3 The primary rhizome condition of the Polygonatum sibiricum seed treated in the step (2) is tested by setting the concentration to be 0mg/L, 10mg/L and 30mg/L, and other parameters are unchanged, and the results are shown in the following table 2:
TABLE 2 gibberellin GA at various concentrations 3 Influence on primary dormancy breaking of Polygonatum sibiricum seed
0mg/L 10mg/L 30mg/L 50mg/L
GA 3 10.20％ 30％ 21.70％ 24％
The test results show that GA 3 Seed ratio GA for inducing germination 1 The germination-inducing seeds have high mildew rate and low later survival rate. And also (b)The embryo axis is too long, so that the nutrition of the embryo is consumed, and the later seedling development is not facilitated.
Comparative example 2:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 4+7 Soaking in the solution for 48h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃;
(3) Soaking the primary rhizome in 300mg/L KT solution for 12h, refrigerating the primary rhizome at 4 ℃ for 30 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedling.
The test of the secondary dormancy of the primary rootstock treated in the step (3) of the comparative example shows that the germination rate of the primary rootstock after the secondary dormancy is released is only 53.33%.
Comparative example 3:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seeds are respectively treated with gibberellin GA with concentration of 10mg/L and 20mg/L 4+7 Soaking in the solution for 72h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃;
(3) Soaking the primary rhizome in a 6- (2-hydroxybenzylamino) purine solution with the concentration of 400mg/L for 24 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the rhizoma polygonati seedling.
The primary rhizome condition of the Polygonatum sibiricum seed treated in the step (2) is tested, and the result shows that the primary rhizome occurrence rate of the Polygonatum sibiricum seed treated in the step (2) is 61.90% (gibberellin concentration is 10 mg/L) and 67.70% (gibberellin concentration is 20 mg/L) respectively.
Comparative example 4:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 4+7 Soaking in the solution for 48h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4deg.C for 30 days, transferring to substrate for cultivation, and breaking secondary dormancy to obtain rhizoma Polygonati seedling.
The test of the secondary dormancy of the primary rootstock treated in the step (3) of the comparative example shows that the germination rate of the primary rootstock after the secondary dormancy is removed is only 33.33%.
Comparative example 5:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 4+7 Soaking in the solution for 48h, transferring to a matrix for culturing, and removing one dormancy to obtain primary rhizome, wherein the soaking temperature is 26 ℃;
(3) Soaking the primary rhizome in 300 mg/L2, 4D solution for 12h, refrigerating the primary rhizome at 4deg.C for 30 days, transferring to matrix culture, and breaking secondary dormancy to obtain rhizoma Polygonati seedling.
The test of the secondary dormancy of the primary rootstock treated in the step (3) of the comparative example shows that the germination rate of the primary rootstock after the secondary dormancy is released is only 6.67%.
Comparative example 6:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 4+7 Soaking in the solution for 48h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 20 ℃ and 25 ℃ respectively;
(3) Soaking the primary rhizome in a 6- (2-hydroxybenzylamino) purine solution with the concentration of 400mg/L for 24 hours, refrigerating the primary rhizome at the temperature of 4 ℃ for 60 days, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain the rhizoma polygonati seedling.
Comparative example 7:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 1 Soaking in the solution for 48h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4deg.C for 30 days, transferring to substrate for cultivation, and breaking secondary dormancy to obtain rhizoma Polygonati seedling.
The condition of the primary rhizome of the polygonatum sibiricum seed treated in the step (2) is tested, and the incidence rate of the primary rhizome of the polygonatum sibiricum seed treated in the comparative example is found to be 74%, and the mildew rate of the seed is found to be 8%.
Comparative example 8:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 4 Soaking in the solution for 48h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4deg.C for 30 days, transferring to substrate for cultivation, and breaking secondary dormancy to obtain rhizoma Polygonati seedling.
The condition of the primary rhizome of the polygonatum sibiricum seed treated in the step (2) is tested, and the incidence rate of the primary rhizome of the polygonatum sibiricum seed treated in the comparative example is found to be 16%, and the mildew rate of the seed is found to be 18.7%.
Comparative example 9:
the method for promoting germination of polygonatum sibiricum seeds of the comparative example comprises the following steps:
(1) Picking immature rhizoma Polygonati fruits in the last ten days of 8 months to the middle ten days, removing impurities, spreading and airing for two days to volatilize partial water, then subpackaging and sealing by using a self-sealing bag, placing at 4 ℃, fermenting at the low temperature for 90d, taking out at the bottom of 10 months, placing in clear water for scrubbing seed coats, collecting seeds sunk under water, and measuring the activity of the seeds; the procedure of measuring the vigor of the polygonatum seeds is the same as that of the example 1;
(2) Rhizoma Polygonati seed is treated with gibberellin GA at concentration of 50mg/L 7 Soaking in the solution for 48h, transferring to matrix culture, and removing dormancy once to obtain primary rhizome, wherein the soaking temperature is 40 ℃;
(3) Soaking the primary rhizome in 300 mg/L6- (2-hydroxybenzylamino) purine solution for 12h, refrigerating the primary rhizome at 4deg.C for 30 days, transferring to substrate for cultivation, and breaking secondary dormancy to obtain rhizoma Polygonati seedling.
The condition of the primary rhizome of the polygonatum sibiricum seed treated in the step (2) is tested, and the occurrence rate of the primary rhizome of the polygonatum sibiricum seed treated in the comparative example is found to be 18.7%, and the mildew rate of the seed is found to be 14.7%.
The above description is merely a preferred embodiment of the present invention, and the scope of the present invention is not limited to the above examples. Modifications and variations which would be obvious to those skilled in the art without departing from the spirit of the invention are also considered to be within the scope of the invention.

Claims (1)

1. A method for promoting germination of polygonatum sibiricum seeds, comprising the steps of:
(1) Soaking the Polygonatum sibiricum seed in gibberellin solution for a set time, and transferring to matrix cultureReleasing the dormancy for the first time to obtain primary rootstocks; the gibberellin solution is GA 1 Is a solution of (a); the concentration of the gibberellin solution is 30-50 mg/L; the soaking time of the polygonatum sibiricum seeds in the gibberellin solution is 24-72 h, and the soaking temperature is 30-40 ℃;
the method for obtaining the Polygonatum sibiricum seed comprises picking immature Polygonatum sibiricum fruit, removing impurities, spreading and airing volatile part of water for two days, sealing at 4 ℃, fermenting at low temperature for 90 days, taking out, placing in clear water to scrub seed coats, and collecting seeds sinking under water;
(2) Soaking the primary rootstock in a growth regulator solution for a set time, refrigerating the primary rootstock, transferring to a substrate for cultivation, and breaking secondary dormancy to obtain rhizoma polygonati seedlings; the growth regulator solution is a 6- (2-hydroxybenzylamino) purine solution; the concentration of the growth regulator solution is 300-400 mg/L; the soaking time of the polygonatum sibiricum seeds in the growth regulator solution is 12-24 hours; the refrigerating temperature of the nascent rhizome is 4 ℃, and the refrigerating time is 30-60 days.
CN202211236885.6A 2022-10-10 2022-10-10 Method for promoting germination of polygonatum sibiricum seeds Active CN115474459B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211236885.6A CN115474459B (en) 2022-10-10 2022-10-10 Method for promoting germination of polygonatum sibiricum seeds

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211236885.6A CN115474459B (en) 2022-10-10 2022-10-10 Method for promoting germination of polygonatum sibiricum seeds

Publications (2)

Publication Number Publication Date
CN115474459A CN115474459A (en) 2022-12-16
CN115474459B true CN115474459B (en) 2024-03-08

Family

ID=84394469

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211236885.6A Active CN115474459B (en) 2022-10-10 2022-10-10 Method for promoting germination of polygonatum sibiricum seeds

Country Status (1)

Country Link
CN (1) CN115474459B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116235762A (en) * 2023-05-12 2023-06-09 江西农业大学 Method for rapidly raising seedlings of polygonatum cyrtonema seeds

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104946535A (en) * 2014-03-26 2015-09-30 中国科学院青岛生物能源与过程研究所 Growth regulator capable of regulating growth and other functions of microalgae, and verification method and application thereof
CN105660219A (en) * 2016-01-20 2016-06-15 贵州大学 Polygonatum cyrtonema seed rapid emerging method
CN109463064A (en) * 2018-12-17 2019-03-15 安徽农业大学 A kind of preprocess method promoting polygonatum cyrtonema seed seedling
CN114041410A (en) * 2021-11-10 2022-02-15 山东省农业科学院 Rapid seedling raising method for polygonatum kingianum seeds

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104946535A (en) * 2014-03-26 2015-09-30 中国科学院青岛生物能源与过程研究所 Growth regulator capable of regulating growth and other functions of microalgae, and verification method and application thereof
CN105660219A (en) * 2016-01-20 2016-06-15 贵州大学 Polygonatum cyrtonema seed rapid emerging method
CN109463064A (en) * 2018-12-17 2019-03-15 安徽农业大学 A kind of preprocess method promoting polygonatum cyrtonema seed seedling
CN114041410A (en) * 2021-11-10 2022-02-15 山东省农业科学院 Rapid seedling raising method for polygonatum kingianum seeds

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
多花黄精初生根茎破除休眠及其成苗的条件研究;陈松树等;《时珍国医国药》;第28卷(第7期);第1748-1750页 *

Also Published As

Publication number Publication date
CN115474459A (en) 2022-12-16

Similar Documents

Publication Publication Date Title
CN103609223B (en) Gibberellin and wet sand bed amass the method that paris polyphylla seed quick-speed germination is bred
CN110100526B (en) Cultivation method for increasing survival rate of thesium chinense
CN115474459B (en) Method for promoting germination of polygonatum sibiricum seeds
CN111886961B (en) Application of gamma-aminobutyric acid as dormancy breaking promoter in breaking seed dormancy
CN100594773C (en) Method for processing sown tendril-leaved fritillary bulb
CN104620718B (en) One breaks the seed dormant method of Herba Digitariae
CN109874446B (en) Cultivation method of macadamia nut seeds
CN109526298B (en) Passion fruit seed treatment method and sowing and breeding method thereof
CN109511534B (en) Berry seed sowing and breeding method
CN113598055B (en) Ainsliaea fragrans champ germplasm improvement method
CN114451240B (en) Method for promoting emergence of polygonatum odoratum seeds
CN101878733B (en) Method for promoting germination of guineagrass seed
CN112616663A (en) Method for greatly shortening planting period of lilium davidii var davidii and rapidly propagating seedlings
CN112243856A (en) Early-maturing late-japonica rice hybrid breeding method
CN107950399A (en) A kind of method for improving Some New True Potato Seed utilization and screening efficiency
CN116530415B (en) Circularly operated polygonatum cyrtonema tissue culture breeding method
CN104620982B (en) Platycrater arguta offspring's method is bred in a kind of cultivation by immature seed
CN111631102B (en) Cultivation method for quick breeding of green Chinese onions
CN112535054B (en) Cultivation method for interplanting coconuts under rubber forest
CN103718964B (en) Seed breeding technology of tripterospermum cordatum
CN114451098B (en) Method for promoting germination of pepper seeds
CN113615343B (en) Method for improving germination rate of belamcanda chinensis seeds
CN115191305B (en) Method for breaking dormancy rapid leaf growth of rhizoma polygonati seeds
CN111328810B (en) Regulator for enhancing root growth activity of transplanted peanut seedlings, method and application
CN111418592B (en) Low-temperature-resistant seed soaking agent for rice in germination stage, preparation method and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20240204

Address after: 410000 No. 1 Nongda Road, Furong District, Changsha City, Hunan Province

Applicant after: Hunan Agricultural University

Country or region after: China

Address before: Room 401, No.101, building 4, workshop of Hunan scientific research achievements transformation center, Longping high tech park, Furong district, Changsha City, Hunan Province 410000

Applicant before: Changsha boguan Biotechnology Co.,Ltd.

Country or region before: China

GR01 Patent grant
GR01 Patent grant