CN115466671B - Anti-flushing liquid nucleic acid amplification product detection device and detection method - Google Patents
Anti-flushing liquid nucleic acid amplification product detection device and detection method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Abstract
The invention discloses a detection device and a detection method for anti-flushing nucleic acid amplification substances, wherein a detection sample is stored through a liquid storage unit, and when the detection sample is required to be detected, a buffer base is connected with the liquid storage unit, so that a liquid storage cavity is communicated with a buffer tank, and the detection sample sequentially flows through the buffer tank, a drainage unit and the detection unit to finish detection; in this in-process, detect the sample before flowing into detecting element, be arranged in depositing liquid cavity and buffer tank all the time, the leakproofness is good, can prevent to detect sample polluted environment, detect the sample and get into after the buffer tank and not directly contact with detecting element simultaneously, but fill up buffer tank earlier, contact with drainage unit again, from this, detect the sample and can overcome gravity and flow into detecting element from bottom to top, obviously this in-process, can avoid the emergence of dashing liquid phenomenon, stop detecting the sample because of the possibility that dashes liquid splashes out in the gap, greatly improved holistic security.
Description
Technical Field
The invention relates to the field of nucleic acid amplification detection, in particular to a detection device and a detection method for anti-flushing liquid nucleic acid amplification substances.
Background
In recent years, with the rapid development of molecular biology techniques, diagnostic methods based on nucleic acid detection have been established in large numbers and widely used in laboratory detection of human diseases; among them, conventional diagnostic methods include isothermal amplification techniques, PCR molecular diagnostic techniques, exponential amplification techniques, and the like.
The isothermal amplification technology is the basis of nucleic acid amplification products, and single-molecule nucleic acid detection can be performed at a constant and low temperature for only 30 minutes. However, the following production scenarios are common in the current common nucleic acid amplification products in China: 1. the operator adds amplification reagents to a tube containing a nucleic acid sample; 2. sealing the test tube; 3. after amplification is finished, opening the test tube and placing the test tube into portable equipment, wherein the portable equipment adds detection liquid into the test tube, or manually placing test paper into the test tube; in the third scenario described above, as long as the detection liquid or the test paper is added into the test tube, the amplified sample in the test tube must be flushed (the liquid in the test tube is splashed out of the test tube after being impacted) under the action of gravitational potential energy, resulting in environmental pollution and extremely low safety.
Disclosure of Invention
The detection device and the detection method for the nucleic acid amplification product of the anti-flushing liquid are provided, so that the problem that the safety is extremely low due to the flushing liquid phenomenon when the nucleic acid amplification product in the prior art is used is solved.
To achieve the purpose, the invention adopts the following technical scheme:
the detection device comprises a buffer base, a liquid storage unit and a detection unit, wherein the buffer base is detachably connected with the liquid storage unit;
the buffer base is provided with a buffer groove, a liquid storage cavity for storing a detection sample is formed in the liquid storage unit, the detection unit is arranged above the buffer groove, and a drainage unit is arranged between the detection unit and the buffer base;
when the buffer base is connected with the liquid storage unit, the liquid storage cavity is communicated with the buffer groove, one end of the drainage unit is abutted to the buffer groove, and the other end of the drainage unit is opposite to the detection unit.
Optionally, the liquid storage unit comprises an amplification unit and a dilution unit which are detachably connected; the amplification unit is provided with an amplification groove for storing a nucleic acid sample and an amplification reagent; the dilution unit is provided with a first communication port and a second communication port which are communicated, and a dilution cavity for storing a dilution reagent is formed between the first communication port and the second communication port;
a movable unit is movably connected in the dilution cavity, and the movable unit seals the first communication port and the second communication port;
when a nucleic acid sample is subjected to amplification reaction, the amplification unit is connected with the dilution unit, the first communication port is opposite to the notch of the amplification groove, and the movable unit blocks the amplification groove from being communicated with the dilution cavity;
when the amplified sample is diluted, the movable unit is pushed in a direction approaching to the amplification unit, and the amplification tank is communicated with the dilution cavity.
Optionally, the movable unit comprises a movable rod, and a first blocking block and a second blocking block are respectively arranged on the movable rod in a protruding mode, wherein the first blocking block is used for blocking the first communication port, and the second blocking block is used for blocking the second communication port;
when the amplified sample is diluted, the first block is pushed in a direction away from the first communication port, and the second block is pushed in a direction away from the second communication port.
Optionally, a first sealing unit is sleeved at the second communication port, and the first sealing unit is abutted with the wall of the dilution cavity;
a second sealing unit is sleeved in the first sealing unit, a connecting hole is formed in the second sealing unit, and the movable unit can movably penetrate through the connecting hole;
the bottom of the buffer groove is convexly provided with a pushing block, and the section size of the pushing block is smaller than the aperture of the connecting hole.
Optionally, the dilution unit is provided with an annular blocking wall part in a protruding manner at the edge of the second communication port, and the drainage unit is arranged between the blocking wall part and the second communication port;
when the buffer base is connected with the liquid storage unit, the notch edge of the buffer groove is abutted to the drainage unit, and the drainage unit is pressed on the dilution unit.
Optionally, the detection unit comprises a detection part, wherein a detection cavity is formed in the detection part and is used for storing detection test paper;
and a detection communication port is formed on one side of the detection cavity, which is close to the drainage unit, and the drainage unit is used for plugging the detection communication port.
Optionally, the detection portion is integrally formed with the dilution unit; an amplification mounting groove is formed between the detection part and the dilution unit at one side close to the amplification unit;
when the amplifying unit is connected with the diluting unit, the groove wall of the amplifying groove is inserted into the amplifying mounting groove.
Optionally, the amplification unit is an elastic amplification housing.
The detection method is applied to the anti-flushing liquid nucleic acid amplification product detection device, the liquid storage cavity stores a detection sample, and the detection method comprises the following steps:
connect the buffering base with deposit the liquid unit, make deposit the liquid cavity with the buffer tank intercommunication, detect the sample flow through in proper order the buffer tank the drainage unit reaches detection unit accomplishes the detection.
A detection method is applied to the anti-flushing liquid nucleic acid amplification product detection device and comprises the following steps:
adding a nucleic acid sample and an amplification reagent into the amplification tank, connecting the amplification unit and the dilution unit to form a liquid storage unit, and shaking uniformly to complete a nucleic acid amplification reaction;
the movable unit is enabled to be communicated with the dilution cavity along the direction close to the amplification unit, and shaking is carried out uniformly to complete dilution;
connect the buffering base with dilute the unit makes dilute cavity with the buffer tank intercommunication, detect the sample flow through in proper order the buffer tank the drainage unit reaches detection unit accomplishes the detection.
Compared with the prior art, the invention has the following beneficial effects:
according to the detection device and the detection method for the anti-flushing nucleic acid amplification product, the detection sample is stored through the liquid storage unit, when the village detection sample is required to be detected, the buffer base is connected with the liquid storage unit, the liquid storage cavity is communicated with the buffer tank, and the detection sample sequentially flows through the buffer tank, the drainage unit and the detection unit, so that detection is completed; in this in-process, detect the sample before flowing into detecting element, be arranged in depositing liquid cavity and buffer tank all the time, the leakproofness is good, can prevent to detect sample polluted environment, detect the sample and get into after the buffer tank and not directly contact with detecting element simultaneously, but fill up buffer tank earlier, contact with drainage unit again, from this, detect the sample and can overcome gravity and flow into detecting element from bottom to top, obviously this in-process, can avoid the emergence of dashing liquid phenomenon, stop detecting the sample because of the possibility that dashes liquid splashes out in the gap, greatly improved holistic security.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are only some embodiments of the invention, and that other drawings can be obtained from these drawings without inventive faculty for a person skilled in the art.
The structures, proportions, sizes, etc. shown in the drawings are shown only in connection with the present disclosure, and are not intended to limit the scope of the invention, since any modification, variation in proportions, or adjustment of the size, etc. of the structures, proportions, etc. should be considered as falling within the spirit and scope of the invention, without affecting the effect or achievement of the objective.
FIG. 1 is a schematic diagram showing the overall structure of a detection device for nucleic acid amplification product of anti-wash liquid according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an explosion structure of a detection device for nucleic acid amplification product of an anti-wash solution according to an embodiment of the present invention;
FIG. 3 is a schematic cross-sectional view of a detection device for nucleic acid amplification product of anti-wash liquid according to an embodiment of the present invention;
FIG. 4 is a schematic view of the partial enlarged structure of FIG. 3 at A;
FIG. 5 is a schematic view of the partial enlarged structure of FIG. 3 at B;
FIG. 6 is a schematic diagram showing a first cross-sectional structure of a detection device for nucleic acid amplification product of an anti-wash solution according to an embodiment of the present invention;
FIG. 7 is a schematic diagram showing a second cross-sectional structure of a detection device for nucleic acid amplification product of an anti-wash solution according to an embodiment of the present invention;
FIG. 8 is a flow chart of a detection method according to a second embodiment of the present invention;
fig. 9 is a flow chart of a detection method according to a third embodiment of the invention.
Illustration of: 10. a buffer base; 11. a buffer tank; 12. a pushing block; 20. a liquid storage unit; 21. a liquid storage cavity; 30. a detection unit; 31. a detection unit; 32. a detection cavity; 33. detecting test paper; 34. detecting a communication port; 40. a drainage unit; 50. an amplification unit; 51. an amplification tank; 60. a dilution unit; 61. a first communication port; 62. a second communication port; 63. a dilution chamber; 64. a blocking wall portion; 65. amplifying the mounting groove; 70. a movable unit; 71. a movable rod; 72. a first block; 73. a second block; 81. a first sealing unit; 82. a second sealing unit; 83. a connection hole; 90. and (3) sealing rings.
Detailed Description
In order to make the objects, features and advantages of the present invention more comprehensible, the technical solutions in the embodiments of the present invention are described in detail below with reference to the accompanying drawings, and it is apparent that the embodiments described below are only some embodiments of the present invention, but not all embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the directions or positional relationships indicated by the terms "upper", "lower", "top", "bottom", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present invention. It is noted that when one component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present.
The technical scheme of the invention is further described below by the specific embodiments with reference to the accompanying drawings.
Referring to fig. 1 to 9, fig. 1 is a schematic diagram of the overall structure of a device for detecting nucleic acid amplification product by using an anti-wash solution according to an embodiment of the present invention, fig. 2 is a schematic diagram of the explosion structure of the device for detecting nucleic acid amplification product by using an anti-wash solution according to an embodiment of the present invention, fig. 3 is a schematic diagram of the cross-sectional structure of the device for detecting nucleic acid amplification product by using an anti-wash solution according to an embodiment of the present invention, fig. 4 is a schematic diagram of the local amplification structure at a position a of fig. 3, fig. 5 is a schematic view of a partial enlarged structure at a position B in fig. 3, fig. 6 is a schematic view of a first cross-sectional structure of a device for detecting a nucleic acid amplification product of a wash solution according to a first embodiment of the present invention, fig. 7 is a schematic view of a second cross-sectional structure of a device for detecting a nucleic acid amplification product of a wash solution according to a first embodiment of the present invention, fig. 8 is a schematic flow chart of a detection method according to a second embodiment of the present invention, and fig. 9 is a schematic flow chart of a detection method according to a third embodiment of the present invention.
Example 1
The anti-flushing nucleic acid amplification product detection device provided by the embodiment of the invention is applied to a nucleic acid detection scene, and the structure of the anti-flushing nucleic acid amplification product detection device is improved, so that the anti-flushing nucleic acid amplification product detection device can prevent the occurrence of flushing phenomenon, and the pollution of detection samples to the environment is effectively avoided.
As shown in fig. 1 to 3, the anti-wash nucleic acid amplification product detection device of the present embodiment includes a buffer base 10, a liquid storage unit 20, and a detection unit 30, where the buffer base 10 is detachably connected to the liquid storage unit 20. The buffer base 10 is provided with a buffer groove 11, a liquid storage cavity 21 for storing a detection sample is formed in the liquid storage unit 20, the detection unit 30 is arranged above the buffer groove 11, and a drainage unit 40 is arranged between the detection unit 30 and the buffer base 10; wherein, drainage unit 40 can be made with the absorbent material such as cotton, and it sets up between buffer tank 11 and detecting element 30, can absorb the detection sample in the buffer tank 11 to under the effect of capillary force, make the detection sample flow into detecting element 30, through setting up the flow mode from bottom to top, avoided the liquid phenomenon to take place, and make the detection sample can more fully contact with detecting element 30, improved the detection precision.
When the buffer base 10 is connected with the liquid storage unit 20, the liquid storage cavity 21 is communicated with the buffer tank 11, one end of the drainage unit 40 is abutted with the buffer tank 11, and the other end of the drainage unit 40 is opposite to the detection unit 30.
Specifically, the anti-flushing liquid nucleic acid amplification product detection device stores a detection sample through the liquid storage unit 20, when the detection sample needs to be detected, the buffer base 10 is connected with the liquid storage unit 20, the liquid storage cavity 21 is communicated with the buffer groove 11, and the detection sample sequentially flows through the buffer groove 11, the drainage unit 40 and the detection unit 30, so that detection is completed; in this process, before flowing into the detecting unit 30, the detecting sample is always located in the liquid storage cavity 21 and the buffer tank 11, so that the sealing performance is good, the detecting sample can be prevented from polluting the environment, meanwhile, after entering the buffer tank 11, the detecting sample is not directly contacted with the detecting unit 30, but is filled in the buffer tank 11 and then contacted with the drainage unit 40, therefore, the detecting sample can flow into the detecting unit 30 from bottom to top against the gravity, the phenomenon of flushing can be avoided obviously in this process, the possibility that the detecting sample splashes out of a gap due to flushing liquid is avoided, and the overall safety is greatly improved.
Further, as shown in fig. 2 to 5, the reservoir unit 20 includes an amplification unit 50 and a dilution unit 60 detachably connected; the amplification unit 50 is provided with an amplification tank 51 for storing a nucleic acid sample and an amplification reagent; the dilution unit 60 is provided with a first communication port 61 and a second communication port 62 which are communicated, and a dilution cavity 63 for storing a dilution reagent is formed between the first communication port 61 and the second communication port 62 of the dilution unit 60; a movable unit 70 is movably connected in the dilution chamber 63, and the movable unit 70 seals the first communication port 61 and the second communication port 62.
Specifically, when the nucleic acid sample undergoes an amplification reaction, the amplification unit 50 is connected to the dilution unit 60, the first communication port 61 is provided opposite to the notch of the amplification well 51, and the movable unit 70 blocks the amplification well 51 from communicating with the dilution chamber 63; when diluting the amplified sample, the movable unit 70 is pushed in a direction approaching the amplification unit 50, and the amplification chamber 51 communicates with the dilution chamber 63. When the amplification reaction is carried out, the amplification reagent and the nucleic acid sample are isolated in the amplification tank 51 by the movable unit 70, so that the contact of the dilution reagent and the nucleic acid sample in advance can be avoided, the detection precision is improved, meanwhile, the movable unit 70 is adopted to realize the blocking and communication between the amplification tank 51 and the dilution cavity 63, and when the amplification reaction reaches a conversion state between the dilution samples, the effect that the amplification sample smoothly flows from the amplification tank 51 to the dilution cavity 63 can be realized, the hydraulic transition is smooth, the transient increase of pressure is avoided, and the overall safety is improved.
In an alternative embodiment, the amplification unit 50 is an elastomeric amplification housing. The operator can pinch the elastic amplification housing to change the internal pressure and flow the amplified sample into the dilution chamber 63, and it should be understood that when the sealability of the reservoir unit 20 is improved by other means, the situation that the amplified sample is not easy to flow into the dilution chamber 63 due to the excessive internal pressure is avoided because the contents can be flowed in the above manner
In an alternative embodiment, as shown in fig. 2, 6 and 7, the movable unit 70 includes a movable rod 71, and a first blocking piece 72 and a second blocking piece 73 are respectively provided on the movable rod 71 in a protruding manner, where the first blocking piece 72 is used for blocking the first communication port 61, and the second blocking piece 73 is used for blocking the second communication port 62; when diluting the amplified sample, the first block 72 is pushed in a direction away from the first communication port 61, and the second block 73 is pushed in a direction away from the second communication port 62.
In other alternative embodiments, the first block 72 and the second block 73 may be fixed to the movable rod 71 by means of adhesion.
Wherein, the first blocking piece 72 and the second blocking piece 73 are both annular. A sealing ring 90 can be sleeved on the outer wall of the first plugging block 72, and the friction force between the cavity wall of the dilution cavity 63 and the first plugging block 72 can be increased by the arrangement of the sealing ring 90, so that the sealing performance is improved.
In a specific embodiment, as shown in fig. 5 to 7, a first sealing unit 81 is sleeved at the second communication port 62, and the first sealing unit 81 abuts against the cavity wall of the dilution cavity 63; the first sealing unit 81 is sleeved with a second sealing unit 82, the second sealing unit 82 is provided with a connecting hole 83, and the movable unit 70 movably penetrates through the connecting hole 83; the bottom of the buffer groove 11 is convexly provided with a pushing block 12, and the section size of the pushing block 12 is smaller than the aperture of the connecting hole 83; the structure reduces the processing difficulty of the dilution unit 60 through the arrangement of the first sealing unit 81 and the second sealing unit 82, so that the whole device is easier to mold. When the test is performed, the buffer base 10 is connected to the dilution unit 60, and the pushing block 12 moves the unit 70 further, so that the diluted sample in the dilution chamber 63 flows into the buffer tank 11 from the gap between the connection hole 83 and the pushing block 12.
In other alternative embodiments, the wall of the dilution chamber 63 may be provided with an extension protruding inwards, and an opening is configured in the middle of the extension, which is equivalent to directly reducing the size of the second communication port 62 to such an extent that the second blocking piece 73 cannot pass through, and at the same time, the pushing piece 12 can pass through the second communication port 62; in this embodiment, the degree of integration is high.
Further, the dilution unit 60 is provided with an annular blocking wall portion 64 protruding from the edge of the second communication port 62, and the drainage unit 40 is disposed between the blocking wall portion 64 and the second communication port 62; when the buffer base 10 is connected to the liquid storage unit 20, the notch edge of the buffer tank 11 abuts against the drain unit 40, and the drain unit 40 is pressed against the dilution unit 60. It should be appreciated that the blocking wall portion 64 can be sleeved outside the buffer base 10 to improve the sealing performance, and at the same time, can also perform a preliminary positioning function on the drainage unit 40, so that the use of additional external parts is saved, and the structure is more compact.
Wherein, the buffer groove 11 is also sleeved with a sealing ring 90, and the outer wall of the buffer groove 11 is tightly contacted with the baffle wall part 64 through the sealing ring 90, thereby improving the tightness.
Further, the detection unit 30 includes a detection portion 31, a detection cavity 32 is formed in the detection portion 31, and the detection cavity 32 is used for storing detection test paper 33; a detection communication port 34 is formed on one side of the detection cavity 32, which is close to the drainage unit 40, and the drainage unit 40 seals the detection communication port 34.
In a specific embodiment, as shown in fig. 4, the detecting portion 31 is integrally formed with the dilution unit 60; an amplification mounting groove 65 is formed between the detection section 31 and the dilution unit 60 on the side close to the amplification unit 50; when the amplification unit 50 is connected to the dilution unit 60, the groove wall of the amplification groove 51 is inserted into the amplification mounting groove 65. It is to be understood that the amplification mounting groove 65 formed by the above arrangement can improve the positioning accuracy of the amplification unit 50, and the integrally formed detection part 31 and dilution unit 60 can further improve the molding accuracy of the amplification mounting groove 65.
In other alternative embodiments, the detecting portion 31 and the diluting unit 60 may be two separate components, and the determination of the relative position may be achieved by a screw or the like.
In summary, the anti-flushing nucleic acid amplification product detection device provided by the embodiment can avoid the flushing phenomenon, eliminates the possibility that the detection sample splashes out of the gap due to flushing, greatly improves the overall safety, and has the advantages of easy processing, compact structure, high precision and the like.
Example two
As shown in fig. 8, the present embodiment provides a detection method applied to the anti-wash nucleic acid amplification product detection apparatus according to the first embodiment, wherein the detection sample is stored in the liquid storage cavity 21, and the steps include:
s101, connecting the buffer base 10 with the liquid storage unit 20, enabling the liquid storage cavity 21 to be communicated with the buffer groove 11, and enabling a detection sample to sequentially flow through the buffer groove 11, the drainage unit 40 and the detection unit 30 to finish detection. The liquid storage unit 20 may be an integral sample storage unit without dilution, where the sample storage unit is provided with an opening, and the opening is provided with a sealing film, a movable sealing rod, and other structures, so that the detection sample can flow into the detection unit 30 from bottom to top.
In summary, the detection method provided in this embodiment refers to the detection device for the nucleic acid amplification product of the anti-flushing liquid in the first embodiment, and the specific structure and effects of the detection device for the nucleic acid amplification product of the anti-flushing liquid in the first embodiment are described, so that the method can avoid the occurrence of the flushing liquid phenomenon, stop the possibility that the detection sample splashes out of the gap due to the flushing liquid, greatly improve the overall safety, and has the advantages of easy processing, compact structure, high precision and the like.
Example III
As shown in fig. 9, the present embodiment provides a detection method applied to the anti-wash nucleic acid amplification product detection apparatus according to the first embodiment, including the steps of:
s201, adding a nucleic acid sample and an amplification reagent into an amplification tank 51, connecting an amplification unit 50 and a dilution unit 60 to form a liquid storage unit 20, and shaking uniformly to complete a nucleic acid amplification reaction;
s202, enabling the movable unit 70 to communicate the amplification groove 51 with the dilution cavity 63 along the direction approaching to the amplification unit 50, and shaking uniformly to finish dilution; at this time, the tip of the movable unit 70 no longer blocks the amplification chamber 51 and the dilution chamber 63, but the movable unit 70 still blocks the second communication port 62.
S023, connecting the buffer base 10 with the dilution unit 60, enabling the dilution cavity 63 to be communicated with the buffer tank 11, and enabling the detection sample to sequentially flow through the buffer tank 11, the drainage unit 40 and the detection unit 30 to finish detection; at this time, the movable unit 70 is pushed up by the buffer base 10, and the diluted sample can flow into the buffer tank 11.
In summary, the detection method provided in this embodiment refers to the detection device for the nucleic acid amplification product of the anti-flushing liquid in the first embodiment, and the specific structure and effects of the detection device for the nucleic acid amplification product of the anti-flushing liquid in the first embodiment are described, so that the method can avoid the occurrence of the flushing liquid phenomenon, stop the possibility that the detection sample splashes out of the gap due to the flushing liquid, greatly improve the overall safety, and has the advantages of easy processing, compact structure, high precision and the like.
The above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (6)
1. The anti-flushing liquid nucleic acid amplification product detection device is characterized by comprising a buffer base (10), a liquid storage unit (20) and a detection unit (30), wherein the buffer base (10) is detachably connected with the liquid storage unit (20);
the buffer base (10) is provided with a buffer groove (11), a liquid storage cavity (21) for storing a detection sample is formed in the liquid storage unit (20), the detection unit (30) is arranged above the buffer groove (11), and a drainage unit (40) is arranged between the detection unit (30) and the buffer base (10); the drainage unit (40) is made of a water-absorbing material;
when the buffer base (10) is connected with the liquid storage unit (20), the liquid storage cavity (21) is communicated with the buffer groove (11), one end of the drainage unit (40) is abutted to the buffer groove (11), and the other end of the drainage unit (40) is opposite to the detection unit (30); during detection, the buffer base (10) is connected with the liquid storage unit (20), the liquid storage cavity (21) is communicated with the buffer groove (11), the buffer groove (11) is filled with a detection sample after the detection sample enters the buffer groove (11), the detection sample is contacted with the drainage unit (40), and the detection sample flows into the detection unit (30) under the action of capillary force; the detection sample sequentially flows through the buffer groove (11), the drainage unit (40) and the detection unit (30), and flows into the detection unit (30) from bottom to top;
the liquid storage unit (20) comprises an amplification unit (50) and a dilution unit (60) which are detachably connected; the amplification unit (50) is provided with an amplification groove (51) for storing a nucleic acid sample and an amplification reagent; the dilution unit (60) is provided with a first communication port (61) and a second communication port (62) which are communicated, and a dilution cavity (63) for storing a dilution reagent is formed between the first communication port (61) and the second communication port (62) of the dilution unit (60);
a movable unit (70) is movably connected in the dilution cavity (63), and the movable unit (70) seals the first communication port (61) and the second communication port (62);
when a nucleic acid sample is subjected to an amplification reaction, the amplification unit (50) is connected with the dilution unit (60), the first communication port (61) is arranged opposite to the notch of the amplification tank (51), and the movable unit (70) blocks the amplification tank (51) from communicating with the dilution cavity (63);
when diluting an amplified sample, the movable unit (70) is pushed in a direction approaching the amplification unit (50), and the amplification tank (51) is communicated with the dilution chamber (63);
the movable unit (70) comprises a movable rod (71), a first blocking block (72) and a second blocking block (73) are respectively arranged on the movable rod (71) in a protruding mode, the first blocking block (72) is used for blocking the first communication port (61), and the second blocking block (73) is used for blocking the second communication port (62);
when diluting the amplified sample, the first block (72) is pushed in a direction away from the first communication port (61), and the second block (73) is pushed in a direction away from the second communication port (62);
the detection unit (30) comprises a detection part (31), a detection cavity (32) is formed in the detection part (31), and the detection cavity (32) is used for storing detection test paper (33);
a detection communication port (34) is formed on one side, close to the drainage unit (40), of the detection cavity (32), and the drainage unit (40) seals the detection communication port (34);
the detection part (31) and the dilution unit (60) are integrally formed; an amplification mounting groove (65) is formed between the detection unit (31) and the dilution unit (60) on the side close to the amplification unit (50);
when the amplification unit (50) is connected with the dilution unit (60), the groove wall of the amplification groove (51) is inserted into the amplification installation groove (65).
2. The anti-flushing nucleic acid amplification product detection device according to claim 1, wherein a first sealing unit (81) is sleeved at the second communication port (62), and the first sealing unit (81) is abutted against the cavity wall of the dilution cavity (63);
a second sealing unit (82) is sleeved in the first sealing unit (81), a connecting hole (83) is formed in the second sealing unit (82), and the movable unit (70) movably penetrates through the connecting hole (83);
the bottom of the buffer groove (11) is convexly provided with a pushing block (12), and the section size of the pushing block (12) is smaller than the aperture of the connecting hole (83).
3. The device for detecting a nucleic acid amplification product by a liquid according to claim 1, wherein the dilution unit (60) has an annular blocking wall portion (64) protruding from an edge of the second communication port (62), and the drainage unit (40) is disposed between the blocking wall portion (64) and the second communication port (62);
when the buffer base (10) is connected with the liquid storage unit (20), the notch edge of the buffer groove (11) is abutted to the drainage unit (40), and the drainage unit (40) is pressed on the dilution unit (60).
4. The device for detecting a nucleic acid amplification product by using a wash solution according to claim 1, wherein the amplification unit (50) is an elastic amplification housing.
5. The detection method, which is applied to the anti-wash nucleic acid amplification product detection apparatus according to any one of claims 1 to 4, wherein the liquid storage cavity (21) stores a detection sample, and the detection method comprises the steps of:
connect buffering base (10) with deposit liquid unit (20), make deposit liquid cavity (21) with buffer tank (11) intercommunication, detect the sample flow through in proper order buffer tank (11), drainage unit (40) and detection unit (30) accomplish the detection.
6. A detection method, which is applied to the anti-wash nucleic acid amplification product detection device according to any one of claims 1 to 4, comprising:
adding a nucleic acid sample and an amplification reagent into the amplification tank (51), connecting the amplification unit (50) and the dilution unit (60) to form a liquid storage unit (20), and shaking uniformly to complete a nucleic acid amplification reaction;
the movable unit (70) is enabled to be communicated with the dilution cavity (63) along the direction approaching to the amplification unit (50), and shaking is carried out to complete dilution;
connect buffering base (10) with dilute unit (60), make dilute cavity (63) with buffer tank (11) intercommunication, detect the sample flow through in proper order buffer tank (11), drainage unit (40) and detection unit (30) accomplish the detection.
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| CN116622493B (en) * | 2023-07-26 | 2023-09-26 | 成都云芯医联科技有限公司 | Microfluidic blood cell detection chip |
| CN117025379B (en) * | 2023-10-09 | 2023-12-15 | 迪飞医学科技(南京)有限公司 | RAPID isothermal amplification nucleic acid detection device and detection method |
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