CN115466671A - Anti-scour liquid nucleic acid amplification product detection device and detection method - Google Patents
Anti-scour liquid nucleic acid amplification product detection device and detection method Download PDFInfo
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- 230000003321 amplification Effects 0.000 title claims abstract description 131
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Abstract
The invention discloses a device and a method for detecting an anti-flushing nucleic acid amplification product, wherein a detection sample is stored through a liquid storage unit, when the detection sample needs to be detected, a buffer base and the liquid storage unit are connected, so that a liquid storage cavity is communicated with a buffer tank, and the detection sample sequentially flows through the buffer tank, a drainage unit and a detection unit to finish detection; in the process, the detection sample is always positioned in the liquid storage cavity and the buffer tank before flowing into the detection unit, the sealing performance is good, the detection sample can be prevented from polluting the environment, and meanwhile, the detection sample is not directly contacted with the detection unit after entering the buffer tank, but the buffer tank is filled firstly and then is contacted with the drainage unit, so that the detection sample can overcome the gravity and flows into the detection unit from bottom to top.
Description
Technical Field
The invention relates to the field of nucleic acid amplification detection, in particular to an anti-scour nucleic acid amplification product detection device and a detection method.
Background
In recent years, with the rapid development of molecular biology techniques, diagnostic methods based on nucleic acid detection have been largely established and widely applied in laboratory detection of human diseases; the conventional diagnostic methods include isothermal amplification technology, PCR molecular diagnostic technology, exponential amplification technology, and the like.
The isothermal amplification technology is the basis of nucleic acid amplification products, and single-molecule nucleic acid detection can be performed at a constant and low temperature within 30 min. However, the following production scenarios are ubiquitous in the current domestic common nucleic acid amplification products: 1. adding an amplification reagent into a test tube filled with a nucleic acid sample by a worker; 2. sealing the test tube; 3. after the amplification is finished, opening the test tube and putting the test tube into portable equipment, and adding detection liquid into the test tube by the portable equipment, or manually putting the test paper into the test tube; in the third scenario, as long as the detection liquid or the test paper is added into the test tube, under the action of gravitational potential energy, the amplified sample in the test tube inevitably has a liquid flushing phenomenon (the liquid in the test tube splashes out of the test tube after being impacted), so that the environment is polluted, and the safety is extremely low.
Disclosure of Invention
Provides a device and a method for detecting an anti-flush nucleic acid amplification product, which solve the problem that the safety of the nucleic acid amplification product in the prior art is extremely low due to the flushing phenomenon when the product is used.
In order to achieve the purpose, the invention adopts the following technical scheme:
a device for detecting an anti-flush nucleic acid amplification product comprises a buffer base, a liquid storage unit and a detection unit, wherein the buffer base is detachably connected with the liquid storage unit;
the buffer base is provided with a buffer groove, a liquid storage cavity for storing a detection sample is formed in the liquid storage unit, the detection unit is arranged above the buffer groove, and a drainage unit is arranged between the detection unit and the buffer base;
when the buffer base with when depositing liquid unit and connecting, deposit the liquid cavity with the buffer slot intercommunication, the one end of drainage unit with the buffer slot butt, the other end of drainage unit with the detecting element sets up relatively.
Optionally, the liquid storage unit comprises an amplification unit and a dilution unit which are detachably connected; the amplification unit is provided with an amplification groove for storing a nucleic acid sample and an amplification reagent; the diluting unit is provided with a first communicating port and a second communicating port which are communicated with each other, and a diluting cavity for storing a diluting reagent is formed between the first communicating port and the second communicating port of the diluting unit;
the dilution cavity is internally and movably connected with a movable unit, and the movable unit blocks the first communicating port and the second communicating port;
when the nucleic acid sample is subjected to amplification reaction, the amplification unit is connected with the dilution unit, the first communication port is arranged opposite to the notch of the amplification groove, and the movable unit blocks the communication between the amplification groove and the dilution cavity;
when the amplified sample is diluted, the movable unit is pushed in a direction close to the amplification unit, and the amplification slot is communicated with the dilution cavity.
Optionally, the movable unit includes a movable rod, the movable rod is respectively provided with a first blocking block and a second blocking block in a protruding manner, the first blocking block is used for blocking the first communication port, and the second blocking block is used for blocking the second communication port;
when diluting the amplified sample, the first blocking piece is pushed in a direction away from the first communicating port, and the second blocking piece is pushed in a direction away from the second communicating port.
Optionally, a first sealing unit is sleeved at the second communication port, and the first sealing unit is abutted against the wall of the dilution cavity;
a second sealing unit is sleeved in the first sealing unit, a connecting hole is formed in the second sealing unit, and the movable unit movably penetrates through the connecting hole;
the tank bottom of buffer slot is protruding to be equipped with and to promote the piece, the cross-sectional dimension that promotes the piece is less than the aperture of connecting hole.
Optionally, an annular wall portion is convexly arranged at the edge of the second communication port of the dilution unit, and the drainage unit is arranged between the wall portion and the second communication port;
when the buffer base with when depositing liquid unit connection, the notch border of dashpot with the drainage unit butt will the drainage unit pressure is located dilute the unit.
Optionally, the detection unit includes a detection portion, a detection cavity is formed in the detection portion, and the detection cavity is used for storing detection test paper;
and a detection communicating opening is formed in one side, close to the drainage unit, of the detection cavity, and the drainage unit blocks the detection communicating opening.
Optionally, the detection portion is integrally formed with the dilution unit; an amplification installation groove is formed between the detection part and the dilution unit on one side close to the amplification unit;
when the amplification unit is connected with the dilution unit, the groove wall of the amplification groove is inserted in the amplification installation groove.
Optionally, the amplification unit is an elastic amplification housing.
A detection method is applied to the device for detecting the anti-scour liquid nucleic acid amplification product, wherein a detection sample is stored in a liquid storage cavity, and the method comprises the following steps:
connect buffer base with deposit the liquid unit, make deposit the liquid cavity with the dashpot intercommunication, the detection sample flows through in proper order the dashpot, the drainage unit reaches the detecting element accomplishes the detection.
A detection method applied to the device for detecting an anti-scour nucleic acid amplification product comprises the following steps:
adding a nucleic acid sample and an amplification reagent into the amplification tank, connecting the amplification unit with the dilution unit to form a liquid storage unit, and shaking up to complete a nucleic acid amplification reaction;
enabling the movable unit to communicate the amplification groove with the dilution cavity along the direction close to the amplification unit, shaking up, and completing dilution;
connect buffer base with dilute the unit, make dilute the cavity with the dashpot intercommunication, the detection sample flows through in proper order the dashpot, drainage unit reaches the detecting element accomplishes the detection.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a device and a method for detecting an anti-flush nucleic acid amplification product, wherein a detection sample is stored through a liquid storage unit, when the detection sample needs to be detected, a buffer base and the liquid storage unit are connected, so that a liquid storage cavity is communicated with a buffer groove, and the detection sample sequentially flows through the buffer groove, a drainage unit and a detection unit to complete detection; in the process, the detection sample is always positioned in the liquid storage cavity and the buffer tank before flowing into the detection unit, the sealing performance is good, the detection sample can be prevented from polluting the environment, meanwhile, the detection sample is not directly contacted with the detection unit after entering the buffer tank, the buffer tank is filled up first and then contacted with the drainage unit, therefore, the detection sample can overcome the gravity and flows into the detection unit from bottom to top, obviously, in the process, the occurrence of a buffer liquid phenomenon can be avoided, the possibility that the detection sample is splashed out from a gap due to the buffer liquid is avoided, and the overall safety is greatly improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without inventive labor.
The structure, proportion, size and the like shown in the drawings are only used for matching with the content disclosed in the specification, so that the person skilled in the art can understand and read the description, and the description is not used for limiting the limit condition of the implementation of the invention, so the method has no technical essence, and any structural modification, proportion relation change or size adjustment still falls within the scope of the technical content disclosed by the invention without affecting the effect and the achievable purpose of the invention.
Fig. 1 is a schematic view of an overall structure of an anti-scour nucleic acid amplification product detection apparatus according to an embodiment of the present invention;
fig. 2 is a schematic diagram of an explosion structure of an apparatus for detecting an anti-scour nucleic acid amplification product according to an embodiment of the present invention;
FIG. 3 is a schematic cross-sectional view of an apparatus for detecting an anti-scour nucleic acid amplification product according to an embodiment of the present invention;
FIG. 4 is an enlarged partial schematic view of FIG. 3 at A;
FIG. 5 is a partial enlarged view of FIG. 3 at B;
FIG. 6 is a schematic diagram of a first cross-sectional structure of an apparatus for detecting an anti-scour nucleic acid amplification product according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of a second cross-sectional structure of an apparatus for detecting an anti-scour nucleic acid amplification product according to an embodiment of the present invention;
FIG. 8 is a flowchart illustrating a second exemplary embodiment of a detection method;
fig. 9 is a schematic flowchart of a detection method according to a third embodiment of the present invention.
Illustration of the drawings: 10. a buffer base; 11. a buffer tank; 12. a pushing block; 20. a liquid storage unit; 21. a liquid storage cavity; 30. a detection unit; 31. a detection unit; 32. a detection cavity; 33. detecting test paper; 34. detecting the communication port; 40. a drainage unit; 50. an amplification unit; 51. an amplification tank; 60. a dilution unit; 61. a first communication port; 62. a second communication port; 63. a dilution cavity; 64. a barrier portion; 65. an amplification mounting groove; 70. a movable unit; 71. a movable rod; 72. a first plugging block; 73. a second plugging block; 81. a first sealing unit; 82. a second sealing unit; 83. connecting holes; 90. and (5) sealing rings.
Detailed Description
In order to make the objects, features and advantages of the present invention more obvious and understandable, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the embodiments described below are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. It should be noted that when one component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present.
The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.
Referring to fig. 1 to 9, fig. 1 is a schematic overall structure diagram of a device for detecting an anti-liquid nucleic acid amplification product according to an embodiment of the present invention, fig. 2 is a schematic explosion structure diagram of the device for detecting an anti-liquid nucleic acid amplification product according to an embodiment of the present invention, fig. 3 is a schematic cross-sectional structure diagram of the device for detecting an anti-liquid nucleic acid amplification product according to an embodiment of the present invention, fig. 4 is a schematic partial enlarged structure diagram of fig. 3 at a, fig. 5 is a schematic partial enlarged structure diagram of fig. 3 at B, fig. 6 is a schematic cross-sectional structure diagram of the device for detecting an anti-liquid nucleic acid amplification product according to an embodiment of the present invention, fig. 7 is a schematic cross-sectional structure diagram of a second device for detecting an anti-liquid nucleic acid amplification product according to an embodiment of the present invention, fig. 8 is a schematic flow diagram of a detection method according to an embodiment of the present invention, and fig. 9 is a schematic flow diagram of a detection method according to a third embodiment of the present invention.
Example one
The anti-flushing nucleic acid amplification product detection device provided by the embodiment of the invention is applied to a nucleic acid detection scene, and the structure of the anti-flushing nucleic acid amplification product detection device is improved, so that the flushing phenomenon can be prevented, and the pollution of a detection sample to the environment is effectively avoided.
As shown in fig. 1 to 3, the apparatus for detecting an anti-flush nucleic acid amplification product of the present embodiment includes a buffer base 10, a liquid storage unit 20, and a detection unit 30, wherein the buffer base 10 is detachably connected to the liquid storage unit 20. The buffer base 10 is provided with a buffer groove 11, a liquid storage cavity 21 for storing a detection sample is formed in the liquid storage unit 20, the detection unit 30 is arranged above the buffer groove 11, and a drainage unit 40 is arranged between the detection unit 30 and the buffer base 10; wherein, drainage unit 40 can be made by absorbent materials such as cotton, and it sets up between buffer tank 11 and detecting element 30, can absorb the testing sample in the buffer tank 11 to under the effect of capillary action, make the testing sample flow in detecting element 30, through setting up the mobile mode from bottom to top, avoided the liquid washing phenomenon to take place, and make the testing sample can contact with detecting element 30 more fully, improved the detection precision.
When buffering base 10 and deposit liquid unit 20 and be connected, deposit liquid cavity 21 and buffer slot 11 intercommunication, the one end and the buffer slot 11 butt of drainage unit 40, the other end and the relative setting of detecting element 30 of drainage unit 40.
Specifically, the anti-flush nucleic acid amplification product detection device stores a detection sample through the liquid storage unit 20, when the detection sample needs to be detected, the buffer base 10 and the liquid storage unit 20 are connected to enable the liquid storage cavity 21 to be communicated with the buffer groove 11, and the detection sample sequentially flows through the buffer groove 11, the drainage unit 40 and the detection unit 30 to complete detection; in the process, the detection sample is always positioned in the liquid storage cavity 21 and the buffer tank 11 before flowing into the detection unit 30, the sealing performance is good, the detection sample can be prevented from polluting the environment, meanwhile, the detection sample does not directly contact with the detection unit 30 after entering the buffer tank 11, the buffer tank 11 is filled up first and then contacts with the drainage unit 40, therefore, the detection sample can flow into the detection unit 30 from bottom to top by overcoming the gravity, obviously, in the process, the liquid buffer phenomenon can be avoided, the possibility that the detection sample is splashed out from gaps due to the liquid buffer is avoided, and the overall safety is greatly improved.
Further, as shown in fig. 2 to 5, the solution storage unit 20 includes an amplification unit 50 and a dilution unit 60 detachably connected; the amplification unit 50 is provided with an amplification groove 51 for storing a nucleic acid sample and an amplification reagent; the dilution unit 60 is provided with a first communication port 61 and a second communication port 62 which are communicated with each other, and a dilution cavity 63 for storing a diluted reagent is formed between the first communication port 61 and the second communication port 62 of the dilution unit 60; the dilution chamber 63 is movably connected with a movable unit 70, and the movable unit 70 blocks the first communication port 61 and the second communication port 62.
Specifically, when the nucleic acid sample is subjected to an amplification reaction, the amplification unit 50 is connected to the dilution unit 60, the first communication port 61 is disposed opposite to the notch of the amplification tank 51, and the movable unit 70 blocks the communication between the amplification tank 51 and the dilution chamber 63; when the amplified sample is diluted, the movable unit 70 is pushed in a direction to approach the amplification unit 50, and the amplification chamber 51 communicates with the dilution chamber 63. When amplification reaction is carried out, amplification reagents and nucleic acid samples are isolated in the amplification tank 51 by the movable unit 70, so that the dilution reagents can be prevented from contacting the nucleic acid samples in advance, the detection precision is improved, meanwhile, the movable unit 70 is adopted to realize the blocking and communication between the amplification tank 51 and the dilution cavity 63, when the amplification reaction is in a conversion state to the dilution samples, the effect that the amplification samples smoothly flow from the amplification tank 51 to the dilution cavity 63 can be realized, the hydraulic transition is smooth, the pressure is prevented from being increased instantaneously, and the integral safety is improved.
In an alternative embodiment, the amplification unit 50 is an elastic amplification housing. The operator may pinch the elastic amplification case to change the internal pressure to flow the amplified sample into the dilution chamber 63, and it should be understood that, when the sealing property of the liquid storage unit 20 is improved in other forms, since the contents may flow in the above manner, the situation that the amplified sample is not easily flowed into the dilution chamber 63 due to the excessively high internal pressure is prevented from occurring
In an alternative embodiment, as shown in fig. 2, 6 and 7, the movable unit 70 includes a movable rod 71, a first blocking block 72 and a second blocking block 73 are respectively protruded on the movable rod 71, the first blocking block 72 is used for blocking the first communication port 61, and the second blocking block 73 is used for blocking the second communication port 62; when the amplified sample is diluted, the first blocking piece 72 is pushed in a direction away from the first communication port 61, and the second blocking piece 73 is pushed in a direction away from the second communication port 62.
In other alternative embodiments, the first and second blocks 72, 73 may be adhesively secured to the movable rod 71.
The first blocking block 72 and the second blocking block 73 are both ring-shaped. A sealing ring 90 can be sleeved on the outer wall of the first blocking block 72, and the arrangement of the sealing ring 90 can increase the friction force between the cavity wall of the diluting cavity 63 and the first blocking block 72, so that the sealing performance is improved.
In a specific embodiment, as shown in fig. 5 to 7, a first sealing unit 81 is sleeved at the second communication port 62, and the first sealing unit 81 abuts against the cavity wall of the dilution cavity 63; the first sealing unit 81 is sleeved with the second sealing unit 82, the second sealing unit 82 is provided with a connecting hole 83, and the movable unit 70 movably penetrates through the connecting hole 83; a pushing block 12 is convexly arranged at the bottom of the buffer groove 11, and the section size of the pushing block 12 is smaller than the aperture of the connecting hole 83; this structure has reduced the processing degree of difficulty of diluting unit 60 through the setting of first sealed unit 81 and second sealed unit 82 for the whole shaping of changeing of device. When the test is performed, the buffer base 10 is connected to the dilution unit 60, and the pushing block 12 moves further to the unit 70, so that the diluted sample in the dilution chamber 63 flows into the buffer slot 11 from the gap between the connecting hole 83 and the pushing block 12.
In other alternative embodiments, the wall of the dilution chamber 63 may be provided with an extension portion protruding inwards, and the middle of the extension portion is provided with an opening, which is equivalent to directly reducing the size of the second communication port 62 to the extent that the second blocking block 73 cannot pass through, and at the same time, the pushing block 12 can pass through the second communication port 62; this embodiment has a high degree of integration.
Furthermore, the dilution unit 60 is provided with an annular blocking wall portion 64 protruding from the edge of the second communication port 62, and the drainage unit 40 is provided between the blocking wall portion 64 and the second communication port 62; when the buffer base 10 is connected to the liquid storage unit 20, the notch edge of the buffer tank 11 abuts against the drainage unit 40, and the drainage unit 40 is pressed against the dilution unit 60. It should be understood that the blocking wall 64 can be sleeved outside the buffering base 10 to improve the sealing performance, and at the same time, can also play a role in preliminary positioning of the drainage unit 40, thereby saving the use of additional external parts and making the structure more compact.
Wherein, the buffer slot 11 is further sleeved with a sealing ring 90, and the outer wall of the buffer slot 11 is in close contact with the blocking wall part 64 through the sealing ring 90, so that the sealing performance is improved.
Further, the detection unit 30 includes a detection portion 31, a detection cavity 32 is formed in the detection portion 31, and the detection cavity 32 is used for storing a detection test paper 33; a detection communicating opening 34 is formed in one side, close to the drainage unit 40, of the detection cavity 32, and the drainage unit 40 blocks the detection communicating opening 34.
In a specific embodiment, as shown in fig. 4, the detection part 31 is integrally formed with the dilution unit 60; an amplification mounting groove 65 is formed between the detection unit 31 and the dilution unit 60 on the side close to the amplification unit 50; when the amplification unit 50 is connected to the dilution unit 60, the groove walls of the amplification tank 51 are inserted into the amplification mounting groove 65. It is understood that the amplification mounting groove 65 formed by the above arrangement can improve the positioning accuracy of the amplification unit 50, and the integrally molded detection part 31 and dilution unit 60 can further improve the molding accuracy of the amplification mounting groove 65.
In other alternative embodiments, the detecting portion 31 and the diluting unit 60 may be two independent elements, and the determination of the relative position may be achieved by a screw connection or the like.
In conclusion, the scour protection liquid nucleic acid amplification product detection device provided by the embodiment can avoid the occurrence of the flushing phenomenon, avoids the possibility that the detection sample is splashed out of the gap due to the flushing, greatly improves the overall safety, and has the advantages of easiness in processing, compact structure, high precision and the like.
Example two
As shown in fig. 8, the present embodiment provides a detection method, which is applied to the apparatus for detecting an anti-flush nucleic acid amplification product according to the first embodiment, wherein a detection sample is stored in the liquid storage cavity 21, and the steps include:
s101, connecting the buffer base 10 with the liquid storage unit 20 to enable the liquid storage cavity 21 to be communicated with the buffer groove 11, and enabling the detection sample to sequentially flow through the buffer groove 11, the drainage unit 40 and the detection unit 30 to finish detection. The sample storage unit 20 may be an integrated sample storage unit without dilution, and the sample storage unit is configured with an opening, and the opening is configured with a sealing film, a movable sealing rod, and the like, so that the detection sample can flow into the detection unit 30 from bottom to top.
In summary, the detection method provided in this embodiment refers to the anti-scour nucleic acid amplification product detection apparatus in the first embodiment, and the specific structure and effect of the anti-scour nucleic acid amplification product detection apparatus are described in the first embodiment, so that the method can avoid the occurrence of the scour phenomenon, prevent the detection sample from spilling out of the gap due to the scour, greatly improve the overall safety, and have the advantages of easy processing, compact structure, high precision, and the like.
EXAMPLE III
As shown in fig. 9, this embodiment provides a detection method applied to the device for detecting an anti-flush nucleic acid amplification product in the first embodiment, including the steps of:
s201, adding a nucleic acid sample and an amplification reagent into the amplification tank 51, connecting the amplification unit 50 with the dilution unit 60 to form a liquid storage unit 20, and shaking up to complete a nucleic acid amplification reaction;
s202, enabling the movable unit 70 to enable the amplification tank 51 to be communicated with the dilution cavity 63 along the direction close to the amplification unit 50, shaking up, and completing dilution; at this time, the distal end of the movable element 70 no longer blocks the amplification chamber 51 and the dilution chamber 63, but the movable element 70 still blocks the second communication port 62.
S023, connecting the buffer base 10 with the dilution unit 60 to enable the dilution cavity 63 to be communicated with the buffer groove 11, and enabling the detection sample to sequentially flow through the buffer groove 11, the drainage unit 40 and the detection unit 30 to finish detection; at this time, the movable unit 70 is pushed open by the buffer base 10, and the diluted sample can flow into the buffer tank 11.
In summary, the detection method provided in this embodiment introduces the anti-flush nucleic acid amplification product detection device in the first embodiment, and the specific structure and effect of the anti-flush nucleic acid amplification product detection device are described in the first embodiment, so that the method can avoid the occurrence of the flushing phenomenon, prevent the possibility that the detection sample is splashed out of the gap due to the flushing, greatly improve the overall safety, and have the advantages of easy processing, compact structure, high precision, and the like.
The above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. The device for detecting the nucleic acid amplification product of the flushing prevention liquid is characterized by comprising a buffer base (10), a liquid storage unit (20) and a detection unit (30), wherein the buffer base (10) is detachably connected with the liquid storage unit (20);
the buffer base (10) is provided with a buffer groove (11), a liquid storage cavity (21) for storing a detection sample is formed in the liquid storage unit (20), the detection unit (30) is arranged above the buffer groove (11), and a drainage unit (40) is arranged between the detection unit (30) and the buffer base (10);
when buffer base (10) with deposit liquid unit (20) and be connected, deposit liquid cavity (21) with buffer slot (11) intercommunication, the one end of drainage unit (40) with buffer slot (11) butt, the other end of drainage unit (40) with detecting element (30) set up relatively.
2. The antisluidic nucleic acid amplification product detection device of claim 1, wherein the liquid storage unit (20) comprises an amplification unit (50) and a dilution unit (60) that are detachably connected; the amplification unit (50) is provided with an amplification groove (51) for storing a nucleic acid sample and an amplification reagent; the dilution unit (60) is provided with a first communication port (61) and a second communication port (62) which are communicated with each other, and a dilution cavity (63) for storing a diluted reagent is formed between the first communication port (61) and the second communication port (62) of the dilution unit (60);
a movable unit (70) is movably connected in the dilution cavity (63), and the movable unit (70) blocks the first communication port (61) and the second communication port (62);
when a nucleic acid sample is subjected to amplification reaction, the amplification unit (50) is connected with the dilution unit (60), the first communication port (61) is arranged opposite to the notch of the amplification groove (51), and the movable unit (70) blocks the communication between the amplification groove (51) and the dilution cavity (63);
when diluting the amplified sample, the movable unit (70) is pushed in a direction to approach the amplification unit (50), and the amplification slot (51) communicates with the dilution chamber (63).
3. The scour-prevention nucleic acid amplification product detection apparatus according to claim 2, wherein the movable unit (70) includes a movable rod (71), a first blocking block (72) and a second blocking block (73) are respectively protruded on the movable rod (71), the first blocking block (72) is used for blocking the first communication port (61), and the second blocking block (73) is used for blocking the second communication port (62);
when diluting the amplified sample, the first blocking piece (72) is pushed in a direction away from the first communication port (61), and the second blocking piece (73) is pushed in a direction away from the second communication port (62).
4. The anti-flush nucleic acid amplification product detection device according to claim 2, wherein a first sealing unit (81) is sleeved at the second communication port (62), and the first sealing unit (81) is abutted against the wall of the dilution cavity (63);
a second sealing unit (82) is sleeved in the first sealing unit (81), a connecting hole (83) is formed in the second sealing unit (82), and the movable unit (70) can movably penetrate through the connecting hole (83);
the bottom of the buffer groove (11) is convexly provided with a pushing block (12), and the section size of the pushing block (12) is smaller than the aperture of the connecting hole (83).
5. The antisludging nucleic acid amplification product detection device according to claim 2, wherein the dilution unit (60) is provided with an annular barrier portion (64) protruding from an edge of the second communication port (62), and the drainage unit (40) is provided between the barrier portion (64) and the second communication port (62);
when buffer base (10) with when depositing liquid unit (20) and being connected, the notch border of dashpot (11) with drainage unit (40) butt will drainage unit (40) pressure is located dilution unit (60).
6. The device for detecting an anti-flush nucleic acid amplification product according to claim 2, wherein the detection unit (30) comprises a detection part (31), a detection cavity (32) is formed in the detection part (31), and the detection cavity (32) is used for storing a detection test paper (33);
one side of the detection cavity (32) close to the drainage unit (40) is provided with a detection communication port (34), and the drainage unit (40) blocks the detection communication port (34).
7. The antisluidic nucleic acid amplification product detection device according to claim 6, wherein the detection section (31) is integrally formed with the dilution unit (60); an amplification mounting groove (65) is formed between the detection part (31) and the dilution unit (60) on the side close to the amplification unit (50);
when the amplification unit (50) is connected to the dilution unit (60), the walls of the amplification chamber (51) are inserted into the amplification installation chamber (65).
8. The antisluidic nucleic acid amplification product detection device of claim 2, wherein the amplification unit (50) is an elastic amplification housing.
9. A detection method, which is applied to the liquid-repellent nucleic acid amplification product detection apparatus according to any one of claims 1 to 8, wherein a detection sample is stored in the liquid storage chamber (21), and the detection method comprises:
connect buffer base (10) with deposit liquid unit (20), make deposit liquid cavity (21) with buffer slot (11) intercommunication, the detection sample flows through in proper order buffer slot (11), drainage unit (40) and detecting element (30), accomplish and detect.
10. A detection method to be applied to the liquid-repellent nucleic acid amplification product detection apparatus according to any one of claims 2 to 8, comprising:
adding a nucleic acid sample and an amplification reagent into the amplification tank (51), connecting the amplification unit (50) with the dilution unit (60) to form a liquid storage unit (20), and shaking up to complete a nucleic acid amplification reaction;
enabling the movable unit (70) to communicate the amplification groove (51) with the dilution cavity (63) along the direction close to the amplification unit (50), shaking up and completing dilution;
connect buffer base (10) with dilute unit (60), make dilute cavity (63) with buffer slot (11) intercommunication, the detection sample flows through in proper order buffer slot (11), drainage unit (40) and detecting element (30), accomplishes the detection.
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