CN103245659B - A kind of suction tube type quick protein detector and using method - Google Patents
A kind of suction tube type quick protein detector and using method Download PDFInfo
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- CN103245659B CN103245659B CN201310148798.XA CN201310148798A CN103245659B CN 103245659 B CN103245659 B CN 103245659B CN 201310148798 A CN201310148798 A CN 201310148798A CN 103245659 B CN103245659 B CN 103245659B
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Abstract
A kind of suction tube type quick protein detector and using method, belong to Life Science Experiment technical field.Sealed formed by the rubber bulb connected successively, body, liquid storage tank, reaction tank, suction nozzle, rubber; Wherein, rubber bulb is closely enclosed within body, and Coomassie brilliant G-250 dye liquor fills in liquid storage tank, and rubber is tamping the top sealing up suction nozzle.During use, pull out rubber envelope, extruding rubber bulb, extrudes liquid storage tank until Coomassie brilliant G-250 dye liquor is pushed to suction nozzle top by Coomassie brilliant G-250 dye liquor, is moved into by suction nozzle in testing sample solution and also slowly loosens rubber bulb to suck testing sample solution; When Coomassie brilliant G-250 dye liquor and testing sample solution are full of reaction tank, whether the color change according to mixed liquor tentatively judges containing protein in testing sample solution, if the color of mixed liquor becomes blueness from taupe brown, then contain protein in testing sample solution, otherwise not containing protein.
Description
Technical field
The present invention relates to a kind of suction tube type quick protein detector and using method, belong to Life Science Experiment technical field.
Background technology
In Life Science Experiment, in a lot of situation, need whether carry out rapid preliminary detection containing protein in solution.During as carried out protein chromatographic purification experiment, need whether containing protein in instant detection chromatographic column effluent liquid, to determine whether collect efflux.The various analytical instrument of usual use or reacted this detection by specified chemical, its shortcoming is that instrument cost is higher, testing result exists hysteresis quality, operation is comparatively loaded down with trivial details.
Summary of the invention
The object of this invention is to provide a kind of suction tube type quick protein detector and using method, by this suction tube type quick protein detector, can convenient, in real time rapid preliminary detection is carried out to the protein in solution.
A kind of suction tube type quick protein detector provided by the present invention, comprises rubber bulb (1), body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6), rubber envelope (7) composition successively; Rubber bulb (1) is closely enclosed within body (2), Coomassie brilliant G-250 dye liquor (4) fills in liquid storage tank (3), body (2), liquid storage tank (3), reaction tank (5) and suction nozzle (6) are connected as a single entity and communicate, rubber envelope (7) closely seals the top of suction nozzle (6), the volume of liquid storage tank (3) is less than the volume of reaction tank (5), and general liquid storage tank (3) volume is the 1/3-2/3 of reaction tank (5) volume.
Described a kind of suction tube type quick protein detector, body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6) are by transparent and seal gastight glass or rigid plastic is made.
Described a kind of suction tube type quick protein detector, preferred liquid storage tank (3) is in spherical, and inside radius is 1.5mm, and reaction tank (5) is ellipsoid shape, and major radius is 3mm, and short radius is 1.5mm, and suction nozzle (6) aperture is 0.5mm.
When using described a kind of prepackage type agarose gel electrophoresis sample-loading buffer, operate in accordance with the following steps:
1. rubber envelope (7) is pulled out; 2. rubber bulb (1) is extruded, Coomassie brilliant G-250 dye liquor (4) is extruded liquid storage tank (3), until Coomassie brilliant G-250 dye liquor (4) is pushed to suction nozzle (6) top, suction nozzle (6) is moved in testing sample solution and also slowly loosens rubber bulb (1) to suck testing sample solution; 3., when Coomassie brilliant G-250 dye liquor (4) is full of reaction tank (5) with testing sample solution, stop sucking testing sample solution; 4. change according to Coomassie brilliant G-250 dye liquor (4) and the color of the mixed liquor of testing sample solution and whether tentatively judge containing protein in testing sample solution, if the color of mixed liquor becomes blueness from taupe brown, then contain protein in testing sample solution, otherwise not containing protein.
Compared with traditional detection method, use the present invention whether to carry out rapid preliminary detection containing protein in solution, without the need to by any instrument, simple operation, can realize real-time detection.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of suction tube type quick protein detector.
In figure 1, rubber bulb, 2, body, 3, liquid storage tank, 4, Coomassie brilliant G-250 dye liquor, 5, reaction tank, 6, suction nozzle, 7, rubber envelope.
Embodiment
Below in conjunction with the drawings and specific embodiments, the invention will be further described:
As shown in Figure 1, suction tube type quick protein detector is sealed 7 formed by rubber bulb 1, body 2, liquid storage tank 3, reaction tank 5, suction nozzle 6, rubber; Rubber bulb 1 is closely enclosed within body 2, and Coomassie brilliant G-250 dye liquor 4 fills in liquid storage tank 3, and body 2, liquid storage tank 3, reaction tank 5 and suction nozzle 6 are connected as a single entity and communicate, and rubber envelope 7 closely seals the top of suction nozzle 6.Body 2, liquid storage tank 3, reaction tank 5, suction nozzle 6 are by transparent and seal gastight glass or plastics are made.Liquid storage tank 3 is in spherical, and inside radius is 1.5mm, and reaction tank 5 is ellipsoid shape, and major radius is 3mm, and short radius is 1.5mm, and suction nozzle 6 aperture is 0.5mm.
When using this suction tube type quick protein detector, operate in accordance with the following steps: 1. pull out rubber envelope 7; 2. extrude rubber bulb 1, Coomassie brilliant G-250 dye liquor 4 is extruded liquid storage tank 3, until Coomassie brilliant G-250 dye liquor 4 is pushed to suction nozzle 6 top, suction nozzle 6 is moved in testing sample solution and also slowly loosen rubber bulb 1 to suck testing sample solution; 3., when Coomassie brilliant G-250 dye liquor 4 is full of reaction tank 5 with testing sample solution, stop sucking testing sample solution; 4. change according to Coomassie brilliant G-250 dye liquor 4 and the color of the mixed liquor of testing sample solution and whether tentatively judge containing protein in testing sample solution, if the color of mixed liquor becomes blueness from taupe brown, then contain protein in testing sample solution, otherwise not containing protein.
Compared with traditional detection method, use this suction tube type quick protein detector whether to carry out rapid preliminary detection containing protein in solution, without the need to by any instrument, simple operation, can realize real-time detection.
Claims (2)
1. a suction tube type quick protein detector, it is characterized in that, comprise rubber bulb (1), body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6), rubber envelope (7) composition successively; Rubber bulb (1) is closely enclosed within body (2), Coomassie brilliant G-250 dye liquor (4) fills in liquid storage tank (3), body (2), liquid storage tank (3), reaction tank (5) and suction nozzle (6) are connected as a single entity and communicate, rubber envelope (7) closely seals the top of suction nozzle (6), and the volume of liquid storage tank (3) is less than the volume of reaction tank (5);
Liquid storage tank (3) volume is the 1/3-2/3 of reaction tank (5) volume;
Body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6) are by transparent and seal gastight glass or rigid plastic is made;
Liquid storage tank (3) is in spherical, and inside radius is 1.5mm, and reaction tank is ellipsoid shape (5), and major radius is 3mm, and short radius is 1.5mm, and suction nozzle (6) aperture is 0.5mm.
2. the using method of a kind of suction tube type quick protein detector of claim 1, is characterized in that, operates in accordance with the following steps:
1. rubber envelope (7) is pulled out; 2. rubber bulb (1) is extruded, Coomassie brilliant G-250 dye liquor (4) is extruded liquid storage tank (3), until Coomassie brilliant G-250 dye liquor (4) is pushed to suction nozzle (6) top, suction nozzle (6) is moved in testing sample solution and also slowly loosens rubber bulb (1) to suck testing sample solution; 3., when Coomassie brilliant G-250 dye liquor (4) is full of reaction tank (5) with testing sample solution, stop sucking testing sample solution; 4. change according to Coomassie brilliant G-250 dye liquor (4) and the color of the mixed liquor of testing sample solution and whether tentatively judge containing protein in testing sample solution, if the color of mixed liquor becomes blueness from taupe brown, then contain protein in testing sample solution, otherwise not containing protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310148798.XA CN103245659B (en) | 2013-04-25 | 2013-04-25 | A kind of suction tube type quick protein detector and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310148798.XA CN103245659B (en) | 2013-04-25 | 2013-04-25 | A kind of suction tube type quick protein detector and using method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103245659A CN103245659A (en) | 2013-08-14 |
| CN103245659B true CN103245659B (en) | 2016-01-13 |
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| CN201310148798.XA Expired - Fee Related CN103245659B (en) | 2013-04-25 | 2013-04-25 | A kind of suction tube type quick protein detector and using method |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111426681A (en) * | 2019-03-22 | 2020-07-17 | 山西瑞亚力生物技术有限公司 | Method for rapidly judging protein elution degree in simple protein purification process |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19708398A1 (en) * | 1997-03-01 | 1998-09-03 | Roland Birke | Pipette with rubber bulb aspirating microscopic organisms, for fixing or reaction |
| DE20214971U1 (en) * | 2002-09-27 | 2002-11-28 | Klocke Verpackungs Service | Device for determining and displaying at least one physical, chemical or biological property of a test liquid |
| DE202004014908U1 (en) * | 2004-07-15 | 2005-03-17 | Levin Felix | Rapid analysis system, to detect and identify substances in a fluid, e.g. urine, comprises a suction vessel combined with one or more reagents which dissolve rapidly to give specific visual reactions |
| CN101067604A (en) * | 2007-06-08 | 2007-11-07 | 上海新波生物技术有限公司 | Multi-element determinand detecting method based on narrow-slit device and detecting device thereof |
| CN101750413A (en) * | 2009-11-09 | 2010-06-23 | 河南工业大学 | Rapid detection method for protein content of foods |
-
2013
- 2013-04-25 CN CN201310148798.XA patent/CN103245659B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19708398A1 (en) * | 1997-03-01 | 1998-09-03 | Roland Birke | Pipette with rubber bulb aspirating microscopic organisms, for fixing or reaction |
| DE20214971U1 (en) * | 2002-09-27 | 2002-11-28 | Klocke Verpackungs Service | Device for determining and displaying at least one physical, chemical or biological property of a test liquid |
| DE202004014908U1 (en) * | 2004-07-15 | 2005-03-17 | Levin Felix | Rapid analysis system, to detect and identify substances in a fluid, e.g. urine, comprises a suction vessel combined with one or more reagents which dissolve rapidly to give specific visual reactions |
| CN101067604A (en) * | 2007-06-08 | 2007-11-07 | 上海新波生物技术有限公司 | Multi-element determinand detecting method based on narrow-slit device and detecting device thereof |
| CN101750413A (en) * | 2009-11-09 | 2010-06-23 | 河南工业大学 | Rapid detection method for protein content of foods |
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| CN103245659A (en) | 2013-08-14 |
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Granted publication date: 20160113 Termination date: 20190425 |