CN103245659A - Suction tube type quick protein detector and application method thereof - Google Patents
Suction tube type quick protein detector and application method thereof Download PDFInfo
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- CN103245659A CN103245659A CN201310148798XA CN201310148798A CN103245659A CN 103245659 A CN103245659 A CN 103245659A CN 201310148798X A CN201310148798X A CN 201310148798XA CN 201310148798 A CN201310148798 A CN 201310148798A CN 103245659 A CN103245659 A CN 103245659A
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Abstract
The invention relates to a suction tube type quick protein detector and an application method of the suction tube type quick protein detector, and belongs to the technical field of life-science experiments. The suction tube type quick protein detector comprises a rubber suction ball, a tube body, a liquid storage tank, a reaction tank, a suction head and a rubber seal, which are connected in sequence, wherein the rubber suction ball is closely sleeved on the tube body, the liquid storage tank is filled with coomassie brilliant blue G-250 staining solution, and the rubber seal is sealed at the top end of the suction head. When the suction tube type quick protein detector is used, the rubber suction ball is extruded, the coomassie brilliant blue G-250 staining solution is squeezed out until to be squeezed to the top end of the suction head, then the suction head is moved to the sample solution to the tested, and the rubber suction ball is slowly relaxed to suck the sample solution to the tested; and when the reaction tank is full of the coomassie brilliant blue G-250 staining solution and the sample solution to be tested, whether the sample solution to be tested contains protein or not is preliminarily judged according to the color change of the mixed solution, and if the color of the mixed solution becomes blue from gray brown, the sample solution to be tested contains protein, otherwise, the sample solution to be tested does not contain protein.
Description
Technical field
The present invention relates to a kind of hydrocone type protein high speed detector and using method, belong to the Life Science Experiment technical field.
Background technology
In Life Science Experiment, need to carry out quick Preliminary detection to whether containing protein in the solution under a lot of situations.As carry out protein chromatography purifying when experiment, need the instant chromatographic column that detects to flow out in the liquid whether contain protein, to determine whether to collect outflow liquid.Usually use various analytical instrument or react to finish this detection by particular chemical, its shortcoming is that instrument cost is higher, there is hysteresis quality in testing result, the operation is comparatively loaded down with trivial details.
Summary of the invention
The purpose of this invention is to provide a kind of hydrocone type protein high speed detector and using method, by this hydrocone type protein high speed detector, can be convenient, in real time the protein in the solution is carried out quick Preliminary detection.
A kind of hydrocone type protein high speed detector provided by the present invention comprises rubber bulb (1), body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6), rubber envelope (7) composition successively; Rubber bulb (1) closely is enclosed within on the body (2), Coomassie brilliant blue G-250 dye liquor (4) is filled with in liquid storage tank (3), body (2), liquid storage tank (3), reaction tank (5) and suction nozzle (6) are connected as a single entity and communicate, rubber envelope (7) closely seals the top of suction nozzle (6), the volume of liquid storage tank (3) is less than the volume of reaction tank (5), and general liquid storage tank (3) volume is the 1/3-2/3 of reaction tank (5) volume.
Described a kind of hydrocone type protein high speed detector, body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6) are by transparent and seal gastight glass or rigid plastic is made.
Described a kind of hydrocone type protein high speed detector, preferred liquid storage tank (3) is spherical, and inside radius is 1.5mm, and reaction tank (5) is the ellipsoid shape, and major radius is 3mm, and short radius is 1.5mm, and suction nozzle (6) aperture is 0.5mm.
When using described a kind of prepackage type agarose gel electrophoresis sample-loading buffer, operate according to following steps:
1. pull out rubber envelope (7); 2. push rubber bulb (1), Coomassie brilliant blue G-250 dye liquor (4) is extruded liquid storage tank (3), be pushed to suction nozzle (6) top until Coomassie brilliant blue G-250 dye liquor (4), suction nozzle (6) moved into also slowly loosen rubber bulb (1) in the testing sample solution to suck testing sample solution; When treating that 3. coomassie light blue G-250 dye liquor (4) and testing sample solution are full of reaction tank (5), stop to suck testing sample solution; 4. tentatively judge whether containing protein in the testing sample solution according to Coomassie brilliant blue G-250 dye liquor (4) and the change color of the mixed liquor of testing sample solution, if the color of mixed liquor becomes blueness by taupe brown, then contain protein in the testing sample solution, otherwise do not contain protein.
Compare with traditional detection method, use the present invention to carry out quick Preliminary detection to whether containing protein in the solution, need not by any instrument, simple operation can realize real-time detection.
Description of drawings
Fig. 1 is the synoptic diagram of hydrocone type protein high speed detector.
Among the figure 1, rubber bulb, 2, body, 3, liquid storage tank, 4, Coomassie brilliant blue G-250 dye liquor, 5, reaction tank, 6, suction nozzle, 7, the rubber envelope.
Embodiment
The invention will be further described below in conjunction with the drawings and specific embodiments:
As shown in Figure 1, hydrocone type protein high speed detector is made up of rubber bulb 1, body 2, liquid storage tank 3, reaction tank 5, suction nozzle 6, rubber envelope 7; Rubber bulb 1 closely is enclosed within on the body 2, and Coomassie brilliant blue G-250 dye liquor 4 is filled with in liquid storage tank 3, and body 2, liquid storage tank 3, reaction tank 5 and suction nozzle 6 are connected as a single entity and communicate, and rubber envelope 7 closely seals the top of suction nozzle 6.Body 2, liquid storage tank 3, reaction tank 5, suction nozzle 6 are by transparent and seal gastight glass or plastics are made.Liquid storage tank 3 is spherical, and inside radius is 1.5mm, and reaction tank 5 is the ellipsoid shape, and major radius is 3mm, and short radius is 1.5mm, and suction nozzle 6 apertures are 0.5mm.
When using this hydrocone type protein high speed detector, operate according to following steps: 1. pull out rubber envelope 7; 2. push rubber bulb 1, Coomassie brilliant blue G-250 dye liquor 4 is extruded liquid storage tank 3, be pushed to suction nozzle 6 tops until Coomassie brilliant blue G-250 dye liquor 4, suction nozzle 6 is moved into also slowly loosen rubber bulb 1 in the testing sample solution to suck testing sample solution; When treating that 3. coomassie light blue G-250 dye liquor 4 and testing sample solution are full of reaction tank 5, stop to suck testing sample solution; 4. tentatively judge whether containing protein in the testing sample solution according to Coomassie brilliant blue G-250 dye liquor 4 and the change color of the mixed liquor of testing sample solution, if the color of mixed liquor becomes blueness by taupe brown, then contain protein in the testing sample solution, otherwise do not contain protein.
Compare with traditional detection method, use this hydrocone type protein high speed detector to carry out quick Preliminary detection to whether containing protein in the solution, need not by any instrument, simple operation can realize real-time detection.
Claims (5)
1. a hydrocone type protein high speed detector is characterized in that, comprises rubber bulb (1), body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6), rubber envelope (7) composition successively; Rubber bulb (1) closely is enclosed within on the body (2), Coomassie brilliant blue G-250 dye liquor (4) is filled with in liquid storage tank (3), body (2), liquid storage tank (3), reaction tank (5) and suction nozzle (6) are connected as a single entity and communicate, rubber envelope (7) closely seals the top of suction nozzle (6), and the volume of liquid storage tank (3) is less than the volume of reaction tank (5).
2. according to a kind of hydrocone type protein high speed detector of claim 1, it is characterized in that liquid storage tank (3) volume is the 1/3-2/3 of reaction tank (5) volume.
3. according to a kind of hydrocone type protein high speed detector of claim 1, it is characterized in that body (2), liquid storage tank (3), reaction tank (5), suction nozzle (6) are by transparent and seal gastight glass or rigid plastic is made.
4. according to a kind of hydrocone type protein high speed detector of claim 1, it is characterized in that liquid storage tank (3) is spherical, inside radius is 1.5mm, and reaction tank is ellipsoid shape (5), and major radius is 3mm, and short radius is 1.5mm, and suction nozzle (6) aperture is 0.5mm.
5. the using method of the arbitrary a kind of hydrocone type protein high speed detector of claim 1-4 is characterized in that, operates according to following steps:
1. pull out rubber envelope (7); 2. push rubber bulb (1), Coomassie brilliant blue G-250 dye liquor (4) is extruded liquid storage tank (3), be pushed to suction nozzle (6) top until Coomassie brilliant blue G-250 dye liquor (4), suction nozzle (6) moved into also slowly loosen rubber bulb (1) in the testing sample solution to suck testing sample solution; When treating that 3. coomassie light blue G-250 dye liquor (4) and testing sample solution are full of reaction tank (5), stop to suck testing sample solution; 4. tentatively judge whether containing protein in the testing sample solution according to Coomassie brilliant blue G-250 dye liquor (4) and the change color of the mixed liquor of testing sample solution, if the color of mixed liquor becomes blueness by taupe brown, then contain protein in the testing sample solution, otherwise do not contain protein.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310148798.XA CN103245659B (en) | 2013-04-25 | 2013-04-25 | A kind of suction tube type quick protein detector and using method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310148798.XA CN103245659B (en) | 2013-04-25 | 2013-04-25 | A kind of suction tube type quick protein detector and using method |
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| Publication Number | Publication Date |
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| CN103245659A true CN103245659A (en) | 2013-08-14 |
| CN103245659B CN103245659B (en) | 2016-01-13 |
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| CN201310148798.XA Expired - Fee Related CN103245659B (en) | 2013-04-25 | 2013-04-25 | A kind of suction tube type quick protein detector and using method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111426681A (en) * | 2019-03-22 | 2020-07-17 | 山西瑞亚力生物技术有限公司 | Method for rapidly judging protein elution degree in simple protein purification process |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19708398A1 (en) * | 1997-03-01 | 1998-09-03 | Roland Birke | Pipette with rubber bulb aspirating microscopic organisms, for fixing or reaction |
| DE20214971U1 (en) * | 2002-09-27 | 2002-11-28 | Klocke Verpackungs Service | Device for determining and displaying at least one physical, chemical or biological property of a test liquid |
| DE202004014908U1 (en) * | 2004-07-15 | 2005-03-17 | Levin Felix | Rapid analysis system, to detect and identify substances in a fluid, e.g. urine, comprises a suction vessel combined with one or more reagents which dissolve rapidly to give specific visual reactions |
| CN101067604A (en) * | 2007-06-08 | 2007-11-07 | 上海新波生物技术有限公司 | Multi-element determinand detecting method based on narrow-slit device and detecting device thereof |
| CN101750413A (en) * | 2009-11-09 | 2010-06-23 | 河南工业大学 | Rapid detection method for protein content of foods |
-
2013
- 2013-04-25 CN CN201310148798.XA patent/CN103245659B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19708398A1 (en) * | 1997-03-01 | 1998-09-03 | Roland Birke | Pipette with rubber bulb aspirating microscopic organisms, for fixing or reaction |
| DE20214971U1 (en) * | 2002-09-27 | 2002-11-28 | Klocke Verpackungs Service | Device for determining and displaying at least one physical, chemical or biological property of a test liquid |
| DE202004014908U1 (en) * | 2004-07-15 | 2005-03-17 | Levin Felix | Rapid analysis system, to detect and identify substances in a fluid, e.g. urine, comprises a suction vessel combined with one or more reagents which dissolve rapidly to give specific visual reactions |
| CN101067604A (en) * | 2007-06-08 | 2007-11-07 | 上海新波生物技术有限公司 | Multi-element determinand detecting method based on narrow-slit device and detecting device thereof |
| CN101750413A (en) * | 2009-11-09 | 2010-06-23 | 河南工业大学 | Rapid detection method for protein content of foods |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111426681A (en) * | 2019-03-22 | 2020-07-17 | 山西瑞亚力生物技术有限公司 | Method for rapidly judging protein elution degree in simple protein purification process |
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