CN109985680A - Modular multichannel sample detection chip assembly suitable for hand-held SPR detector - Google Patents
Modular multichannel sample detection chip assembly suitable for hand-held SPR detector Download PDFInfo
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- CN109985680A CN109985680A CN201910335975.2A CN201910335975A CN109985680A CN 109985680 A CN109985680 A CN 109985680A CN 201910335975 A CN201910335975 A CN 201910335975A CN 109985680 A CN109985680 A CN 109985680A
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
Abstract
The invention discloses a kind of modular multichannel sample detection chip assemblies suitable for hand-held SPR detector, including Multi-channel liquid sample collection storage device, multichannel sensing chip and hand-held SPR detector shell attachment device, liquid sample storage device includes storage device shell and multiple acquisition device storage chambers, multichannel sensing chip includes substrate and gold-plated sheet glass, and hand-held SPR detector shell attachment device includes the sensing chip installation shell of the rectangle of lower end opening setting.The present invention can be rapidly completed to be matched with the installation of hand-held SPR detector, and is completed liquid sample collecting, reagent mixing and the output of Multi-channel liquid sample, cooperation hand-held SPR detector and completed detection.
Description
Technical field
The present invention relates to a kind of micro-control stream chip more particularly to a kind of modularization suitable for hand-held SPR detector are more
Channel sample detection chip assembly.
Background technique
Surface plasma resonance (SPR) is a kind of physical optics phenomenon.In two kinds of different refractivity (refractive
Index on transparent medium interface) (such as glass and water), it is situated between when Ray Of Light is incident on low-refraction from high refractive index medium
Matter, light will occur to reflect and reflect.When incidence angle increases to a certain particular value, refraction angle be equal to 90 °, this time along with
The tangent direction in interface is projected, and incidence angle at this time is known as critical angle.If incidence angle is more than critical angle, incident ray will not
Another transfer can be entered, and be all reflected back toward in incident medium, experiences total internal reflection.
In fact, although whole incident light is reflected, a kind of electromagnetic field being evanescent wave can pass through contacting permeation to low folding
It penetrates in rate medium, energy is exponentially decayed.If plating one layer of metallic film (general gold-plated film or silverskin), metal in interface
The free electron of film surface is excited by incident light and generates charge oscillation, and then forms surface plasma.Adjustment light enters
When firing angle or wavelength are to a certain appropriate value, surface plasma is equal with the frequency of evanescent wave and wave number, and energy just occurs for the two
Coupling forms surface plasma resonance.The total reflection condition of interface will be destroyed when resonance, and incident laser energy is transferred to table
In surface plasma wave, sharply decline in the air so as to cause intensity of reflected light, decaying total reflection phenomenon is presented.Wherein make anti-
It penetrates the incident light angle that light completely disappears and is known as resonance angle (SPR angle).Resonance angle can be with the medium of metal film surfaces
The change of refractive index and change, and refractive index variation it is directly proportional to the quality for the molecule for being incorporated in metal surface.Therefore pass through
Analyze resonance angle, so that it may obtain the information of intermolecular interaction.
SPR sensorgram technology, which has without label, to surface characteristic and substance, changes sensitivity, in real time, quickly and easily realizes
The features such as automation.The technology has been widely used in life science, clinical diagnosis, drug screening, food safety, environmental monitoring
Equal fields, test object includes albumen, nucleic acid, hormone, toxin, pesticide, cell, microorganism etc..
Application No. is the patents of CN2016213963168 to disclose a kind of hand-held SPR bio-detector, including shell,
It equipped with light incidence component, light-receiving component and light for receiving the sending of light incidence component and is reflexed to light and is connect in shell
The light reflection subassembly of component is received, light reflection subassembly includes reflecting prism, the multichannel sensing on the reflecting surface of reflecting prism
Chip, multichannel sensing chip include substrate and gold-plated sheet glass, and the substrate is equipped with loading slot, negative pressure chamber and is used for
The sample runner that loading slot is connected with negative pressure chamber.The present invention is by light incidence component, light-receiving component and light reflection subassembly
It is fixed in shell, wavelength, angle and the intensity of incident light can be fixed, avoid the SPP instrument of large scale business in the market
Turntable design, overall structure is more simple, easily operated, so that product of the present invention is small in size.
In above-mentioned patent, on the one hand, when testing liquid sample is added, need to take out multichannel sensing chip, and
Testing liquid sample is instilled in loading slot under exposed environments, it is inconvenient, it also will cause testing liquid sample contamination.Meanwhile
Due to generally requiring to store in actual work to liquid sample to be measured, the subsequent extracting solution from liquid sample storage device again
Body sample carries out SPR detection.On the other hand, existing hand-held SPR detector is only capable of once carrying out SPR for a sample
Detection, detection efficiency are lower.
Therefore be badly in need of it is a kind of can install easy to operate, directly disposably extracted from multiple sample storage devices a variety of
Liquid sample, and the device of liquid sample collecting, reagent mixing and Multi-channel liquid sample output services is completed, without sudden and violent
Liquid sample is injected one by one in dew environment.
Summary of the invention
The object of the present invention is to provide a kind of modular multichannel sample detection chips suitable for hand-held SPR detector
Component can be rapidly completed and match with the installation of hand-held SPR detector, and complete liquid sample collecting, reagent mixing with
The output of Multi-channel liquid sample, cooperation hand-held SPR detector complete detection.
The present invention adopts the following technical solutions:
A kind of modular multichannel sample detection chip assembly suitable for hand-held SPR detector, including Multi-channel liquid sample
This acquisition and storage device, multichannel sensing chip and hand-held SPR detector shell attachment device；
Multi-channel liquid sample collection storage device, including multiple liquid sample collectors and liquid sample storage device；
The upper end opening of liquid sample collector is collector sample tap, and liquid sample collector is internally provided with storage chamber,
The lower end of liquid sample collector is provided with the collector outlet be connected with storage chamber, is provided with acquisition on collector sample tap
Device sample tap plugging device is provided with collector outlet plugging device on collector outlet；
Liquid sample storage device, including storage device shell, storage device enclosure interior are provided with multiple acquisition device storages
Chamber, each liquid sample collecting device are all made of removably and are correspondingly arranged in each acquisition device storage chamber, each adopt
Acquisition means storage chamber lower end is provided with collector outlet connecting tube, and each collector outlet connecting tube passes through setting and exists
The U-shaped channel of storage device enclosure interior is connected with the interface arrangement for connecting with SPR inlet sealing and connecting device, corresponding
Collector outlet connecting tube, U-shaped channel and interface arrangement conducting, be provided with interface plugging device in interface arrangement；Each
It is provided with connecting pipe head accommodation groove below interface arrangement, the two sides of connecting pipe head accommodation groove offer sliding groove, connecting tube
The sliding shoe that head is arranged by connecting pipe head two sides is slidably connected with connecting pipe head accommodation groove, and SPR note is provided in connecting pipe head
The connecting cavity that inlet sealing and connecting device shape matches；
Multichannel sensing chip, including substrate and gold-plated sheet glass, being horizontally disposed on the substrate has multiple groups sample channel, often
Group sample is by including sample reserve tank, sample circulation groove, contact chamber and the sample accumulator tank sequentially turned on, sample storage
The opening of slot and sample accumulator tank is respectively positioned on upper surface of base plate, and sample circulation groove and contact chamber are located inside substrate, on substrate also
Equipped with negative pressure chamber, negative pressure chamber is connected with sample accumulator tank, and is provided in the communicating passage between negative pressure chamber and sample accumulator tank
Obstruct mechanism；Contact chamber lower part is provided with opening and the interior sealed set that is open has gold-plated sheet glass；
Hand-held SPR detector shell attachment device, the sensing chip of the rectangle including the setting of lower end opening install shell, sensing
The front and rear sides panel lower end of chip installation shell is provided with sliding block, the sliding slot of sliding block and hand-held SPR detector shell upper end
Matching；Sensing chip is installed housing upper surface and is offered respectively with multiple sample reserve tanks and sample accumulator tank corresponding position
Sample injection port and sample outlet port are provided with inlet sealing and connecting device in sample injection port, are arranged in sample outlet port
There is outlet port sealing and connecting device, is set between inlet sealing and connecting device and outlet port sealing and connecting device and housing upper surface
It is equipped with the spring in compressive state, in its natural state, sample injection port passes through inlet sealing and connecting device and sample to spring
The sealing connection of this reserve tank, sample outlet port are connected to by outlet port sealing and connecting device with the sealing of sample accumulator tank.
The liquid sample collector is tubular, and collector sample tap inner surface is provided with internal screw thread, collector sampling
Mouth plugging device is cylindrical sealing-plug, is provided with and the matched outer spiral shell of collector sample tap internal screw thread on the outside of sealing-plug
Line, sealing-plug are threadedly coupled with collector sample tap；The middle part of sealing-plug is axially disposed threaded hole, is provided in threaded hole
Push rod, the lower part of push rod are provided with thread segment, and the diameter of thread segment is greater than rod diameter, and the lower end of thread segment is connected with and stores
The rubber stopper that chamber matches, storage chamber are slidably connected with storage chamber.
Filter device is provided in the storage chamber, filter device includes filter holder and the first filter screen, filtering branch
Frame includes the mounting ring to match with storage chamber, the first filter screen is provided in mounting ring, there are two branch for the setting of mounting ring lower part
The outer surface of leg, mounting ring is provided with rubber layer.
The outlet plugging device and interface plugging device is all made of rubber film；Collector outlet connecting tube is perpendicular
Straight setting and end be provided with sharp portion, and acquisition device storage chamber bottom is additionally provided with to match with collector outlet outer diameter
The shape of limiting slot, limiting slot and collector outlet matches.
The acquisition device storage inner cavity surface is provided with internal screw thread, the outer surface of upper setting of liquid sample collector
There is external screw thread, liquid sample collector is threadedly coupled with liquid sample storage device, when liquid sample collector is revolved by screw thread
When going to least significant end, collector outlet is located in limiting slot and sharp portion punctures rubber film, collector outlet connecting tube
It is connected with storage chamber.
The opening of the sample reserve tank is circle, and inlet sealing and connecting device includes being arranged in sample injection port
Injection connecting tube, the lower seal for injecting connecting tube is arranged with rubber ring, and the internal diameter of rubber ring is greater than opening for sample reserve tank
The diameter of mouth, in its natural state, rubber ring contacts to form sealing spring with the substrates into intimate of sample reserve tank surrounding；Sample returns
The opening for receiving slot is circle, and outlet port sealing and connecting device includes the extraction connecting tube in sample outlet port, extracts connecting tube out
Lower seal be arranged with rubber ring, the internal diameter of rubber ring is greater than the diameter of the opening of sample accumulator tank, and spring is in nature
Under, rubber ring contacts to form sealing with the substrates into intimate of sample accumulator tank surrounding.
The top of the connecting pipe head matches with cylindrical cavity, and the upper surface of connecting pipe head is inclined-plane, connecting tube
Head is internally provided with truncated cone-shaped connecting cavity；The upper end of injection connecting tube in sample injection port is protruded and housing upper surface, and
The upper end for injecting connecting tube is truncated cone-shaped, and the upper end of the extraction connecting tube in sample outlet port is protruded and housing upper surface, and
The upper end for extracting connecting tube out is truncated cone-shaped.
The front and rear sides panel inner surface lower end of the sensing chip installation shell is provided with multichannel sensing chip and leads
Rail, sliding is provided with multichannel sensing chip bracket on multichannel sensing chip guide rail, and multichannel sensing chip is arranged in multi-pass
In road sensing chip bracket, gold-plated sheet glass lower surface is contacted with reflecting prism upper surface in multichannel sensing chip；Multichannel
Sensing chip guide rail includes the rail brackets of rectangle, and the side of rail brackets offers the guide-track groove of right low left high, guide-track groove
Left upper part and external conductor of the left end by rail brackets, the left end lower part of guide-track groove are provided with vertical limiting slot, limit
The height of slot is consistent with the height of guide-track groove right end；Multichannel sensing chip bracket includes that there are four the rectangle branch of side plate composition
Frame, the lower end of four side plates, which extends internally, is provided with horizontal supporting portion, side plate and rear side on front side of multichannel sensing chip bracket
The left and right ends of side plate are provided with sliding axle, and the width of the diameter and guide-track groove and limiting slot of sliding axle matches, guide-track groove
Width be sequentially reduced from left to right, and the width of guide-track groove right end and limiting slot is of same size.
The outlet port interconnecting piece is connected with negative pressure source by hose, and negative pressure source uses peristaltic pump.
The two sides of the storage device shell are also vertically provided with elastic extension item, the inner surface setting of elastic extension item
There is arc convex；The front and rear sides panel outer surface of sensing chip installation shell is additionally provided with vertical liquid sample storage device
Locating slot, the lower end of liquid sample storage device locating slot are provided with liquid sample storage device location hole.
The present invention first uses liquid sample collector to acquire liquid sample, and liquid sample is then stored in liquid by above-mentioned method
In body sample storage device；Then multichannel sensing chip is mounted in hand-held SPR detector shell attachment device, and will
Hand-held SPR detector shell attachment device is connect with existing hand-held SPR detector shell, is finally stored liquid sample and is filled
It sets and is connect with hand-held SPR detector shell attachment device, whole process forms modularization installation, has merged fluid sample and has adopted
Collection, reagent mixing and Multi-channel liquid sample output services, being capable of the completion detection of quick-fit hand-held SPR detector.This hair
It is bright that there is clever structure, simple advantage is assembled, is overcome.
Detailed description of the invention
Fig. 1 is the structure chart of Multi-channel liquid sample collection storage device in the present invention；
Fig. 2 is the structure chart of liquid sample collecting device in the present invention；
Fig. 3 is the structure chart of liquid sample storage device in the present invention；
Fig. 4 is the structural schematic diagram of existing hand-held SPR detector shell in the present invention；
Fig. 5 is the structural schematic diagram of multichannel sensing chip in the present invention；
Fig. 6 is the side structure schematic view for the multichannel sensing chip being arranged in sensing chip bracket；
Fig. 7 is the present invention looks up structural representation for the multichannel sensing chip being arranged in sensing chip bracket；
Fig. 8 is the structural schematic diagram of multichannel sensing chip guide rail；
Fig. 9 is that the positional relationship of the existing hand-held SPR detector shell on the outside of multichannel sensing chip and reflecting prism is illustrated
Figure.
Specific embodiment
The present invention is made with detailed description below in conjunction with drawings and examples:
As shown in Figures 1 to 9, the modular multichannel sample detection core of the present invention suitable for hand-held SPR detector
Piece component, including Multi-channel liquid sample collection storage device, multichannel sensing chip are connected with hand-held SPR detector shell
Device；
Multi-channel liquid sample collection storage device, including multiple liquid sample collectors and liquid sample storage device；For
Liquid sample is carried out to sample collector to sample, and obtained liquid sample is stored, while being examined with multichannel sample
It surveys chip and realizes fast and reliable connection；
The liquid sample collecting device is sampled for carrying out liquid sample to sample collector, and to obtained liquid
Sample is stored.Liquid sample collecting device includes multiple liquid sample collectors 1, and the upper end of liquid sample collector 1 is opened
Mouth is collector sample tap, and liquid sample collector 1 is internally provided with storage chamber 2, the lower end setting of liquid sample collector 1
There is the collector outlet 3 be connected with storage chamber 2, be provided with collector sample tap plugging device 5 on collector sample tap, acquires
Collector outlet plugging device 4 is provided on device outlet 3.Sample collector can be led liquid sample by collector sample tap
Enter in storage chamber 2, and be sealed collector sample tap using collector sample tap plugging device 5, cooperation collector goes out sample
Mouth plugging device 4, can effectively seal against storage liquid sample.
In the present embodiment, liquid sample collector 1 is tubular, and collector sample tap inner surface is provided with internal screw thread, is acquired
Device sample tap plugging device 5 is cylindrical sealing-plug, is provided on the outside of sealing-plug and matches with collector sample tap internal screw thread
External screw thread, sealing-plug is threadedly coupled with collector sample tap, can effectively improve sealing performance.The middle part of sealing-plug is along axial direction
It is provided with threaded hole, push rod 6 is provided in threaded hole, the lower part of push rod 6 is provided with thread segment 7, and the diameter of thread segment 7, which is greater than, to be pushed away
6 diameter of bar, the lower end of thread segment 7 are connected with the rubber stopper 8 to match with storage chamber 2, and storage chamber 2 is slidably connected with storage chamber 2.
Due to first is that needing to drive liquid sample to SPR inlet sealing and connecting device 26, depending merely in subsequent liquid detection process
Gravity realizes that the outflow of liquid sample is ineffective, second is that subsequent need to mix liquid sample with detection reagent, depends merely on
It shakes and a large amount of bubbles is also easy to produce by liquid sample collector 1, mixed effect is bad.Therefore, the present invention is by rubber stopper 8 and push rod
6 are effectively combined with sealing-plug, use convenient for follow-up work personnel.In view of portability of the invention, in sample phase
Entire push rod 6 can be arranged in sealing-plug by thread segment 7, when needing using push rod 6, push rod 6 can be rotated down, make spiral shell
Line section 7 and sealing-plug are detached from, since 6 diameter of push rod of 7 top of thread segment is less than threaded hole, can according to actual use demand according to
Certain pressure derives downwards push rod 6, and liquid sample is extruded storage chamber 2 using rubber stopper 8.In the present embodiment, under storage chamber 2
Portion is inversed taper platform shape 9, is converged downwards under the effect of gravity convenient for liquid sample.
The liquid sample storage device, including storage device shell 10, the setting of 10 interior parallel of storage device shell
There are multiple acquisition device storage chambers 11, liquid sample collecting device is all made of removably and is correspondingly arranged in acquisition device storage
In chamber 11, each 11 lower end of acquisition device storage chamber is provided with collector outlet connecting tube 12, each collector outlet
Connecting tube 12 passes through the U-shaped channel 13 being arranged in inside storage device shell 10 and is connected with for being tightly connected with SPR inlet
The interface arrangement 14 that device 26 connects, corresponding collector outlet connecting tube, U-shaped channel 13 and interface arrangement conducting, each
14 lower end of interface arrangement is provided with connecting pipe head accommodation groove 23, and the two sides of connecting pipe head accommodation groove 23 offer sliding groove, even
Pipe joint 24 is slidably connected by the sliding shoe 25 that 24 two sides of connecting pipe head are arranged with connecting pipe head accommodation groove 23, connecting pipe head 24
Inside it is provided with the connecting cavity that 26 shape of SPR inlet sealing and connecting device matches.
Liquid sample storage device is convenient for for storing to multiple liquid sample collectors 1 equipped with liquid sample
Carry conveying, while the transmission channel as SPR inlet sealing and connecting device 26 and liquid sample storage device, be convenient for
SPR detector is used cooperatively.In the present embodiment, outlet plugging device 4 and interface plugging device 15 are all made of rubber film.It adopts
Storage outlet connecting tube 12 is vertically arranged and end is provided with sharp portion, and acquisition device storage chamber bottom is additionally provided with and acquires
The shape of the limiting slot that device outlet outer diameter matches, limiting slot and collector outlet matches.Acquisition device storage chamber 11
Inner surface is provided with internal screw thread, and the outer surface of upper of liquid sample collector 1 is provided with external screw thread, liquid sample collector 1 with
Liquid sample storage device is threadedly coupled.The liquid sample equipped with liquid sample is adopted when liquid sample storage device is only used only
When storage 1 is stored, it is only necessary to be connected the two by screw thread, without rotating liquid sample collector 1 to bottom.
It, will when needing that SPR inlet sealing and connecting device 26 is connected with liquid sample memory using liquid sample storage device
When liquid sample collector 1 passes through screw thread rotation to least significant end, collector outlet 3 is positioned in limiting slot and sharp portion punctures
Rubber film, collector outlet connecting tube 12 and storage chamber 2 are connected at this time.
Since there may be certain impurity in fluid sample, in order to improve Detection accuracy, the present invention is in storage chamber 2
It is inside provided with filter device 14, filter device 14 includes filter holder and the first filter screen, and filter holder includes and 2 phase of storage chamber
Matched mounting ring is provided with the first filter screen in mounting ring, and there are two supporting leg 18, the appearances of mounting ring for the setting of mounting ring lower part
Face is provided with rubber layer.Mounting ring and its rubber layer of outer surface setting, can effectively improve filter effect, prevent unfiltered
Liquid sample is flowed out from the gap between 2 inner wall of mounting ring and storage chamber.Meanwhile the design of supporting leg 18 may make the first filter screen energy
Access effective limit.In the present invention, filter device 14 is changeable type, can be replaced not according to liquid sample filtration needs
With the filter screen in filter hole aperture.
Interface arrangement is internally provided with the cylindrical cavity 21 that upper surface is inclined-plane, and interface plugging device 15 is arranged in cylinder
21 lower end surface of shape cavity.The top of connecting pipe head 24 matches with cylindrical cavity 21, and the upper surface of connecting pipe head 24 is inclined-plane,
Or it is provided with sharp portion, connecting pipe head 24 is internally provided with truncated cone-shaped connecting cavity 22, the shape and SPR of truncated cone-shaped connecting cavity 22
26 shape of inlet sealing and connecting device matches.The settable flexible rubber layer of the inner surface of truncated cone-shaped connecting cavity 22, so that SPR
The protruding portion of inlet sealing and connecting device 26 is fitted closely with truncated cone-shaped connecting cavity 22, guarantees certain airtightness, cooperates SPR
Detector internal negative pressure mechanism sucks liquid sample in the sample detection unit inside micro-fluidic chip.The lesser U-shaped of internal diameter
The setting in channel 13 can effectively control the injection rate of liquid sample, and liquid sample is prevented to be tightly connected by SPR inlet
Injection rate is excessive when device 26 injects.
In the present embodiment, the middle part of storage chamber 2 is connected with reagent adsorption layer 20, reagent adsorption layer 20 can be adsorbed in advance with
The corresponding detection reagent of fluid sample.The porous elastic material that sponge etc. has adsorption function can be used in reagent adsorption layer 20.When
After liquid sample collects storage chamber 2, liquid sample can be sufficiently with the detection reagent in reagent adsorption layer 20 in reagent adsorption layer
Mixing in 20, the fluid sample for being mixed with detection reagent flow out after rubber stopper 8 and filter device 14 squeeze, can be precipitated compared with
For the saliva sample of clarification, bubble-free and impurity, corresponding SPR inlet sealing and connecting device finally is flowed into through filter device 14
26, it has been efficiently completed the acquisition of multiple fluid samples and the mixing with detection reagent, has improved sample treatment efficiency, it can one
Multiple liquid templates are directly injected into different SPR inlet sealing and connecting devices 26 and detected by secondary property.
It, respectively will be in liquid sample storage device when the present invention is attached with SPR inlet sealing and connecting device 26
Several connecting pipe head accommodation grooves 23 alignment SPR inlet sealing and connecting device 26 is simultaneously pressed, SPR inlet sealing and connecting device
26 protruding portion fits closely the truncated cone-shaped connecting cavity 22 being arranged by the inside of flexible rubber layer and connecting pipe head 24, together
When, connecting pipe head 24 will move upwards, and pass through the inclined-plane or sharp portion bursting interface plugging device 15 of end, finally to connect
Testing liquid in mouth device 14 flows into corresponding SPR inlet sealing and connecting device 26 and is detected.For the ease of liquid-like
This storage device is effectively connect with SPR inlet sealing and connecting device 26, and the two sides of storage device shell 10 are also vertically provided with
Elastic extension item 26, the inner surface of elastic extension item are provided with arc convex 27.Elastic extension item 26 and arc convex 27 can
By the frictional force and position-limiting action generated at elastic clamping and arc convex 27, guarantee that liquid sample storage device and SPR are infused
Inlet sealing and connecting device 26 is reliably connected.
Multichannel sensing chip, including substrate 313 and gold-plated sheet glass 312, the substrate 313 is horizontally disposed multiple groups
Sample channel, every group of sample by include the sample reserve tank 314, sample circulation groove 315, contact chamber 316 that sequentially turn on
And sample accumulator tank 317, the opening of sample reserve tank 314 and sample accumulator tank 317 are respectively positioned on 313 upper surface of substrate, sample flow
Through slot 315 and contact chamber 316 are located inside substrate 313, and 316 lower part of contact chamber is provided with opening and the interior sealed set that is open has plating
Golden sheet glass 312.Negative pressure chamber 318 is additionally provided on substrate 313, negative pressure chamber 318 and sample accumulator tank 317 are connected, and negative pressure chamber 318
Barrier mechanism 319 is provided in communicating passage between sample accumulator tank 317.
The sensing chip of hand-held SPR detector shell attachment device, the rectangle including the setting of lower end opening installs shell
301, the front and rear sides panel lower end of sensing chip installation shell 301 is provided with sliding block, sliding block and existing hand-held SPR detector
The sliding slot of shell upper end matches；After sliding slot grafting by sliding block and existing hand-held SPR detector shell upper end, multichannel is passed
Gold-plated 312 lower surface of sheet glass and intracorporal 307 upper surface of reflecting prism of shell of existing hand-held SPR detector connect in sense chip
Touching.It offers sensing chip bracket in sensing chip installation 301 left-hand face of shell to import and export, in sensing chip bracket inlet and outlet
It is provided with removable cover 325 to be opened/closed,
In order to avoid existing hand-held SPR detector needs to take out multichannel sensing chip 8 when detecting, and in exposed environments
Lower that testing liquid sample is instilled the defects of loading slot, sensing chip installs 301 front and rear sides inner surface of shell in the present invention
It is symmetrically arranged with multichannel sensing chip guide rail 320, sliding is provided with multichannel sensing core on multichannel sensing chip guide rail 320
Plate rack 321, multichannel sensing chip are arranged in multichannel sensing chip bracket 321；Multichannel sensing chip can pass through
Multichannel sensing chip bracket 321 slides on multichannel sensing chip guide rail 320, passes through multichannel sensing chip bracket 321
Inlet and outlet are put into or take out, after multichannel sensing chip is installed in place, gold-plated 312 following table of sheet glass in multichannel sensing chip
Face is contacted with 307 upper surface of reflecting prism in existing hand-held SPR detector.
In the present embodiment, rectangle of the multichannel sensing chip guide rail 320 including being symmetrically arranged at front and rear sides is led
Rail bracket, the side of rail brackets offer the guide-track groove 322 of right low left high, and the left end of guide-track groove 322 passes through rail brackets
Left upper part and external conductor, the left end lower part of guide-track groove 322 are provided with vertical limiting slot 323, and the bottom end of limiting slot 323 is high
It spends consistent with the height of 322 right end of guide-track groove；Multichannel sensing chip bracket 321 includes that there are four the rectangle branch of side plate composition
Frame, the lower end of four side plates, which extends internally, is provided with horizontal supporting portion, and 321 front side side plate of multichannel sensing chip bracket is with after
The left and right ends of side side plate are provided with sliding axle 324, the diameter of sliding axle 324 and the width of guide-track groove 322 and limiting slot 323
Match.The width of guide-track groove 322 is sequentially reduced from left to right, and the width of the width of 322 right end of guide-track groove and limiting slot 323
It spends identical.
In use, first multichannel sensing chip is put into multichannel sensing chip bracket 321, multichannel sensing chip branch
Four side plates of frame 321 can carry out good limit, the lower end setting of four side plates to four sides of multichannel sensing chip
Horizontal supporting portion can to four of multichannel sensing chip bottom surface it is external carry out excellent supports, prevent multichannel sensing chip
It is fallen from multichannel sensing chip bracket 321.Then by the right end of multichannel sensing chip bracket 321 from guide-track groove 322
Left end opening portion is put into guide-track groove 322, and makes two sliding axles 324 difference on 321 right side of multichannel sensing chip bracket
In the guide-track groove 322 of the rail brackets of front and rear sides, multichannel sensing chip bracket 321, multi-pass are then elapsed to the right
Road sensing chip bracket 321 will gradually develop to horizontal direction, when the right end of multichannel sensing chip bracket 321 reaches guide rail
After the right end of slot 322, two sliding axles 324 in 321 left side of multichannel sensing chip bracket are placed individually into vertical limit
In the slot 323 of position, multichannel sensing chip bracket 321 reaches horizontality at this time and position is fixed, it is ensured that multichannel sensing chip
In gold-plated 312 lower surface of sheet glass contacted with 307 upper surface of reflecting prism.
Sensing chip install 301 upper surface of shell and with 317 corresponding position of multiple sample reserve tanks 314 and sample accumulator tank
Place offers sample injection port and sample outlet port respectively, and inlet sealing and connecting device, sample are provided in sample injection port
Outlet port sealing and connecting device, inlet sealing and connecting device and outlet port sealing and connecting device and shell are provided in outlet port
The spring in compressive state is provided between upper surface, in its natural state, sample injection port is sealed spring by inlet
Attachment device is connected to the sealing of sample reserve tank 314, and sample outlet port passes through outlet port sealing and connecting device and sample accumulator tank
Sealing connection.
Sensing chip install 301 upper surface of shell and with 317 corresponding position of multiple sample reserve tanks 314 and sample accumulator tank
Place offers sample injection port and sample outlet port respectively, and inlet sealing and connecting device 326, sample are provided in sample injection port
Outlet port sealing and connecting device 327 is provided in product outlet port, inlet sealing and connecting device 326 and outlet port are tightly connected dress
It sets and is provided with the spring 328 in compressive state between 327 and 1 upper surface of shell, spring 328 in its natural state, infuse by sample
Entrance is connected to by inlet sealing and connecting device 326 with the sealing of sample reserve tank 314, and sample outlet port is sealed by outlet port
Attachment device 327 is connected to the sealing of sample accumulator tank 317.
The opening of sample reserve tank 314 is circle, and inlet sealing and connecting device 326 includes being arranged in sample injection port
Injection connecting tube 326-1, injection connecting tube 326-1 lower seal be arranged with rubber ring 326-2, rubber ring 326-2's is interior
Diameter is greater than the diameter of the opening of sample reserve tank 314, spring 328 in its natural state, rubber ring 326-2 and sample reserve tank
The substrates into intimate of 314 surroundings contacts to form sealing, can smoothly enter into sample with the liquid sample guaranteed in sample storage device
In reserve tank 314, and entered in sample accumulator tank 317 under the suction function that negative pressure chamber 318 generates.Further, work as sample
Several liquid sample output ends are set on corresponding injection connecting tube 326-1 and are downwardly applied to pressure in this storage device
When, downward pressure will cooperate the elastic force of spring 328, it is ensured that rubber ring 326-2 and the substrate of 314 surrounding of sample reserve tank are tight
Contiguity touching forms sealing.
The opening of sample accumulator tank 317 is circle, and outlet port sealing and connecting device 327 includes being arranged in sample outlet port
Extraction connecting tube 327-1, outlet port sealing and connecting device 327 includes the extraction connecting tube 327-1 in sample outlet port, take out
The lower seal of connecting tube 327-1 is arranged with rubber ring 327-2 out, and the internal diameter of rubber ring 326-2 is greater than sample accumulator tank 317
The diameter of opening, in its natural state, the substrates into intimate of rubber ring 326-2 and 317 surrounding of sample accumulator tank contacts shape to spring 328
At sealing, in order to which the negative pressure of negative pressure chamber 318 can drive liquid sample to flow.
Meanwhile for avoid in special circumstances 318 negative pressure of negative pressure chamber correctly liquid sample can not be driven to flow, extract out connecting tube
327-1 is connected with negative pressure source by hose, and negative pressure source is the setting of spare negative pressure, such as uses peristaltic pump.
Fluid sample is being injected by user by injection connecting tube 326-1 by inlet sealing and connecting device 326
In corresponding sample reserve tank 314, fluid sample circulates out of sample reserve tank 314 through sample under the action of negative pressure chamber 318
Slot 315, contact chamber 316 enter in sample accumulator tank 317, when liquid sample flows to contact chamber 316, if with gold-plated glass
Fixed substance interaction on piece 312, then the molecular mass for combining gold-plated 312 surface of sheet glass changes, and draws
Spr signal variation is played, if spr signal will not be caused to change there is no when interaction, carried out by the signal of SPR
Differentiate.
It is effectively connect for the ease of liquid sample storage device with SPR inlet sealing and connecting device 26, storage device shell
The two sides of body 10 are also vertically provided with elastic extension item 26, and the inner surface of elastic extension item is provided with arc convex 27.Elasticity is prolonged
Liquid can be guaranteed by the frictional force and position-limiting action generated at elastic clamping and arc convex 27 by stretching item 26 and arc convex 27
Body sample storage device is reliably connected with SPR inlet sealing and connecting device 26.Prolong with elasticity in liquid sample storage device
It stretches item 26 and arc convex 27 is corresponding, be additionally provided with vertical liquid sample storage device on sensing chip installation shell 301
Locating slot 329, the lower end of liquid sample storage device locating slot 329 are provided with liquid sample storage device location hole 330.It is convenient for
Sample storage device is fixed on 1 top of shell, downward pressure is continuously applied to injection connecting tube 326-1.
In the present embodiment, upper end protrusion and 1 upper surface of shell of the injection connecting tube 326-1 in sample injection port, and infuse
The upper end for entering connecting tube 326-1 is truncated cone-shaped, upper end protrusion and the shell 1 of the extraction connecting tube 327-1 in sample outlet port
Upper surface, and the upper end for extracting connecting tube 327-1 out is truncated cone-shaped.Cone match can guarantee to inject connecting tube 326-1 and sample
The effective connection and sealing of liquid sample output end in this storage device.
In the use of the present invention, first acquiring liquid sample with liquid sample collector, then liquid sample is done as above-mentioned
Method is stored in liquid sample storage device；Multichannel sensing chip is then mounted on the connection of hand-held SPR detector shell
In device, and hand-held SPR detector shell attachment device is connect with existing hand-held SPR detector shell, finally by liquid
Body sample storage device is connect with hand-held SPR detector shell attachment device, and whole process forms modularization installation, fusion
Liquid sample collecting, reagent mixing and Multi-channel liquid sample output services, can quick-fit hand-held SPR detector it is complete
At detection.
Claims (10)
1. a kind of modular multichannel sample detection chip assembly suitable for hand-held SPR detector, it is characterised in that: including
Multi-channel liquid sample collection storage device, multichannel sensing chip and hand-held SPR detector shell attachment device；
Multi-channel liquid sample collection storage device, including multiple liquid sample collectors and liquid sample storage device；
The upper end opening of liquid sample collector is collector sample tap, and liquid sample collector is internally provided with storage chamber,
The lower end of liquid sample collector is provided with the collector outlet be connected with storage chamber, is provided with acquisition on collector sample tap
Device sample tap plugging device is provided with collector outlet plugging device on collector outlet；
Liquid sample storage device, including storage device shell, storage device enclosure interior are provided with multiple acquisition device storages
Chamber, each liquid sample collecting device are all made of removably and are correspondingly arranged in each acquisition device storage chamber, each adopt
Acquisition means storage chamber lower end is provided with collector outlet connecting tube, and each collector outlet connecting tube passes through setting and exists
The U-shaped channel of storage device enclosure interior is connected with the interface arrangement for connecting with SPR inlet sealing and connecting device, corresponding
Collector outlet connecting tube, U-shaped channel and interface arrangement conducting, be provided with interface plugging device in interface arrangement；Each
It is provided with connecting pipe head accommodation groove below interface arrangement, the two sides of connecting pipe head accommodation groove offer sliding groove, connecting tube
The sliding shoe that head is arranged by connecting pipe head two sides is slidably connected with connecting pipe head accommodation groove, and SPR note is provided in connecting pipe head
The connecting cavity that inlet sealing and connecting device shape matches；
Multichannel sensing chip, including substrate and gold-plated sheet glass, being horizontally disposed on the substrate has multiple groups sample channel, often
Group sample is by including sample reserve tank, sample circulation groove, contact chamber and the sample accumulator tank sequentially turned on, sample storage
The opening of slot and sample accumulator tank is respectively positioned on upper surface of base plate, and sample circulation groove and contact chamber are located inside substrate, on substrate also
Equipped with negative pressure chamber, negative pressure chamber is connected with sample accumulator tank, and is provided in the communicating passage between negative pressure chamber and sample accumulator tank
Obstruct mechanism；Contact chamber lower part is provided with opening and the interior sealed set that is open has gold-plated sheet glass；
Hand-held SPR detector shell attachment device, the sensing chip of the rectangle including the setting of lower end opening install shell, sensing
The front and rear sides panel lower end of chip installation shell is provided with sliding block, the sliding slot of sliding block and hand-held SPR detector shell upper end
Matching；Sensing chip is installed housing upper surface and is offered respectively with multiple sample reserve tanks and sample accumulator tank corresponding position
Sample injection port and sample outlet port are provided with inlet sealing and connecting device in sample injection port, are arranged in sample outlet port
There is outlet port sealing and connecting device, is set between inlet sealing and connecting device and outlet port sealing and connecting device and housing upper surface
It is equipped with the spring in compressive state, in its natural state, sample injection port passes through inlet sealing and connecting device and sample to spring
The sealing connection of this reserve tank, sample outlet port are connected to by outlet port sealing and connecting device with the sealing of sample accumulator tank.
2. the modular multichannel sample detection chip assembly according to claim 1 suitable for hand-held SPR detector,
It is characterized by: the liquid sample collector is tubular, collector sample tap inner surface is provided with internal screw thread, and collector takes
Sample mouth plugging device is cylindrical sealing-plug, is provided with and the matched outer spiral shell of collector sample tap internal screw thread on the outside of sealing-plug
Line, sealing-plug are threadedly coupled with collector sample tap；The middle part of sealing-plug is axially disposed threaded hole, is provided in threaded hole
Push rod, the lower part of push rod are provided with thread segment, and the diameter of thread segment is greater than rod diameter, and the lower end of thread segment is connected with and stores
The rubber stopper that chamber matches, storage chamber are slidably connected with storage chamber.
3. the modular multichannel sample detection chip assembly according to claim 2 suitable for hand-held SPR detector,
It is characterized by: being provided with filter device in the storage chamber, filter device includes filter holder and the first filter screen, filtering
Bracket includes the mounting ring to match with storage chamber, the first filter screen is provided in mounting ring, there are two the settings of mounting ring lower part
The outer surface of supporting leg, mounting ring is provided with rubber layer.
4. the modular multichannel sample detection chip assembly according to claim 3 suitable for hand-held SPR detector,
It is characterized by: the outlet plugging device and interface plugging device is all made of rubber film；The connection of collector outlet
Pipe is vertically arranged and end is provided with sharp portion, and acquisition device storage chamber bottom is additionally provided with and collector outlet outer diameter phase
The shape of the limiting slot matched, limiting slot and collector outlet matches.
5. the modular multichannel sample detection chip assembly according to claim 4 suitable for hand-held SPR detector,
It is characterized by: the acquisition device storage inner cavity surface is provided with internal screw thread, the outer surface of upper of liquid sample collector
It is provided with external screw thread, liquid sample collector is threadedly coupled with liquid sample storage device, when liquid sample collector passes through spiral shell
When line is rotated to least significant end, collector outlet is located in limiting slot and sharp portion punctures rubber film, and collector outlet connects
Adapter tube is connected with storage chamber.
6. the modular multichannel sample detection chip assembly according to claim 5 suitable for hand-held SPR detector,
It is characterized by: the opening of the sample reserve tank is circle, inlet sealing and connecting device includes that setting is injected in sample
Injection connecting tube in mouthful, the lower seal for injecting connecting tube are arranged with rubber ring, and the internal diameter of rubber ring is greater than sample reserve tank
Opening diameter, in its natural state, rubber ring contacts to form sealing spring with the substrates into intimate of sample reserve tank surrounding；Sample
The opening of this accumulator tank is circle, and outlet port sealing and connecting device includes the extraction connecting tube in sample outlet port, and extraction connects
The lower seal of adapter tube is arranged with rubber ring, and the internal diameter of rubber ring is greater than the diameter of the opening of sample accumulator tank, and spring is in nature
Under state, rubber ring contacts to form sealing with the substrates into intimate of sample accumulator tank surrounding.
7. the modular multichannel sample detection chip assembly according to claim 6 suitable for hand-held SPR detector,
It is characterized by: the top of the connecting pipe head matches with cylindrical cavity, the upper surface of connecting pipe head is inclined-plane, connection
Tube head is internally provided with truncated cone-shaped connecting cavity；The upper end of injection connecting tube in sample injection port is protruded and housing upper surface,
And the upper end for injecting connecting tube is truncated cone-shaped, the upper end of the extraction connecting tube in sample outlet port is protruded and housing upper surface,
And the upper end for extracting connecting tube out is truncated cone-shaped.
8. the modular multichannel sample detection chip assembly according to claim 7 suitable for hand-held SPR detector,
It is characterized by: the front and rear sides panel inner surface lower end of the sensing chip installation shell is provided with multichannel sensing chip
Guide rail, sliding is provided with multichannel sensing chip bracket on multichannel sensing chip guide rail, and multichannel sensing chip is arranged more
In the sensing chip bracket of channel, gold-plated sheet glass lower surface is contacted with reflecting prism upper surface in multichannel sensing chip；Multi-pass
Road sensing chip guide rail includes the rail brackets of rectangle, and the side of rail brackets offers the guide-track groove of right low left high, guide-track groove
Left end by the left upper parts of rail brackets and external conductor, the left end lower part of guide-track groove is provided with vertical limiting slot, limits
The height of position slot is consistent with the height of guide-track groove right end；Multichannel sensing chip bracket includes that there are four the rectangle branch of side plate composition
Frame, the lower end of four side plates, which extends internally, is provided with horizontal supporting portion, side plate and rear side on front side of multichannel sensing chip bracket
The left and right ends of side plate are provided with sliding axle, and the width of the diameter and guide-track groove and limiting slot of sliding axle matches, guide-track groove
Width be sequentially reduced from left to right, and the width of guide-track groove right end and limiting slot is of same size.
9. the modular multichannel sample detection chip assembly according to claim 8 suitable for hand-held SPR detector,
It is characterized by: the outlet port interconnecting piece is connected with negative pressure source by hose, negative pressure source uses peristaltic pump.
10. the modular multichannel sample detection chipset according to claim 9 suitable for hand-held SPR detector
Part, it is characterised in that: the two sides of the storage device shell are also vertically provided with elastic extension item, the interior table of elastic extension item
Face is provided with arc convex；The front and rear sides panel outer surface of sensing chip installation shell is additionally provided with vertical liquid sample and deposits
Storage device locating slot, the lower end of liquid sample storage device locating slot are provided with liquid sample storage device location hole.
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Application publication date: 20190709 |