CN106925359A - Micro grade is than loading micro-fluidic chip system and upper quadrat method - Google Patents
Micro grade is than loading micro-fluidic chip system and upper quadrat method Download PDFInfo
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- CN106925359A CN106925359A CN201710264363.XA CN201710264363A CN106925359A CN 106925359 A CN106925359 A CN 106925359A CN 201710264363 A CN201710264363 A CN 201710264363A CN 106925359 A CN106925359 A CN 106925359A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/18—Transport of container or devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0609—Holders integrated in container to position an object
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/14—Means for pressure control
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Abstract
This application discloses a kind of micro grade than loading micro-fluidic chip system and upper quadrat method, including：Micro-fluidic chip, including microchannel, multiple injection ports and at least one outlet, the injection port and outlet are respectively communicated with the two ends of the microchannel；Power set, are connected between the injection port and power source, and the power source is driven using air pressure, Piezoelectric Driving or ultrasonic wave drive.Present invention also offers a kind of upper quadrat method.The invention provides a set of simple system, the decorum can be equipped with different micro fluidic chip units according to application demand, realize the processes such as loading, mixing, reaction, separation, the detection of liquid, disclosure satisfy that micro-fluidic chip is commercialized demand, and the checking requirement of laboratory level, with application prospect higher.
Description
Technical field
The application belongs to microfluidic art, and more particularly to a kind of micro grade is than loading micro-fluidic chip system and upper
Quadrat method.
Background technology
Microfluidic chip technology (Microfluidics) is biology, chemistry, the sample preparation of medical analysis process, anti-
Should, separate, detection etc. basic operation unit be integrated on one piece of chip of micro-meter scale, be automatically performed analysis overall process.Due to
It in the great potential in the field such as biology, chemistry, medical science, have been developed as a biology, chemistry, medical science, fluid, electronics,
The brand-new research field of the subject crossings such as material, machinery.
The features such as due to its small volume, low reagent consumption and Highgrade integration, increasing researcher starts to close
Note micro-fluidic chip., it is necessary to inject liquid on chip, there is related biochemical reaction in use in micro-fluidic chip,
Detected, so as to be applied to different fields.The equipment of micro-fluidic chip loading is coordinated to include syringe pump, wriggle at present
The means such as pump, compression pump and manipulator.The traditional means such as syringe pump, peristaltic pump and compression pump, liquid is continuous operation, even if micro-
Liquid volume is less needed for fluidic chip, if using above-mentioned loading means, because liquid is continuously promoted, actual consumption amount is still
It is very big, it is impossible to play the advantage of micro-fluidic chip.Manipulator can carry out loading according to actual demand amount, then still need
Specified location in liquid driven to micro-fluidic chip could be completed corresponding reaction and detected, in micro-fluidic chip by power
On, the action can be driven using malleation, negative pressure or centrifugal force.
Various samples etc. are generally involved during biochemistry detection to mix than (non-equivalent), could complete target determinand value
Detection.Using manipulator (on the premise of Manual quantitative loading), in addition it is also necessary to provide the grade of stabilization than power source, could realize
The ratio that waits of loading liquid mixes or reaction, and in the market or in laboratory, shortage can quickly realize the whole of the function
System is united.
The content of the invention
It is existing to overcome it is an object of the invention to provide a kind of micro grade than loading micro-fluidic chip system and upper quadrat method
There is the deficiency in technology.
To achieve the above object, the present invention provides following technical scheme：
The embodiment of the present application discloses a kind of micro grade than loading micro-fluidic chip system, including：
Micro-fluidic chip, including microchannel, multiple injection ports and at least one outlet, the injection port and outlet point
The two ends of the microchannel are not communicated in；
Power set, are connected between the injection port and power source, and the power source is driven using air pressure, Piezoelectric Driving
Or ultrasonic wave drives.
Preferably, in above-mentioned micro grade is than loading micro-fluidic chip system, shape between the injection port and microchannel
Into there is liquid storage unit.
Preferably, in above-mentioned micro grade is than loading micro-fluidic chip system, the power set include driving fixture to
And jointing, the jointing is connected to the power source, described to drive fixture to offer at least one through hole, each
The through hole corresponds to be communicated in an injection port respectively, and the jointing is inserted in one end of the through hole.
Preferably, in above-mentioned micro grade is than loading micro-fluidic chip system, the gear of annular is protruded out in the through hole
Circle, is extruded with packing ring, the opening of the external diameter more than the injection port of the packing ring between the back-up ring and micro-fluidic chip surface
Internal diameter.
Preferably, in above-mentioned micro grade is than loading micro-fluidic chip system, the internal diameter of the injection port is less than described
The internal diameter of liquid storage unit, the liquid storage unit is communicated in the lower section of the injection port.
Preferably, in above-mentioned micro grade is than loading micro-fluidic chip system, the power set can be taken out with one
The hollow cavity of liquid is taken, one end of the hollow cavity is connected to the power source, and the other end is communicated in the injection port, described
Power source is pneumatic supply, and the bottom of the hollow cavity protrudes out the Liquid extracting and release structure of back taper, the Liquid extracting
There is the through hole for being connected with the injection port and hollow cavity respectively with release structure.
Preferably, in above-mentioned micro grade than in loading micro-fluidic chip system, power set connect with micro-fluidic chip
Packing ring is provided between contacting surface.
Preferably, in above-mentioned micro grade is than loading micro-fluidic chip system, the micro-fluidic chip include up and down according to
First chip of secondary superposition, the second chip and the 3rd chip, the injection port and outlet are opened on first chip, institute
Microchannel is stated to be formed between second chip and the first chip, run through institute between the second chip and the 3rd chip or up and down
State the second chip.
Accordingly, the embodiment of the present application also discloses a kind of upper quadrat method of micro grade than loading micro-fluidic chip system,
Including：
Quantitative target liq is extracted and delayed into hollow cavity by s1, power set under pneumatic supply suction function
Deposit；
S2, the injection port that the Liquid extracting of hollow cavity bottom and release structure are aligned micro-fluidic chip；
S3, in the presence of pneumatic supply malleation, the target liq in hollow cavity is pushed into injection port；
S4, continuation pressure barotropic gas, are moved by the quantitative target liq of gas push in microchannel.
Compared with prior art, the advantage of the invention is that：The invention provides a set of simple system, the decorum can
Different micro fluidic chip units are equipped with according to application demand, realize that the loading of liquid, mixing, reaction, separation, detection were waited
Journey, disclosure satisfy that micro-fluidic chip is commercialized demand, and the checking of laboratory level is required, with application prospect higher.
Brief description of the drawings
In order to illustrate more clearly of the embodiment of the present application or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments described in application, for those of ordinary skill in the art, on the premise of not paying creative work,
Other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 show the structural representation of micro-fluidic chip in the specific embodiment of the invention；
Fig. 2 show the first chip and the connection diagram for driving fixture in first embodiment of the invention；
Fig. 3 show the structural representation of jointing in first embodiment of the invention；
Fig. 4 show the sectional view of power set in second embodiment of the invention；
Fig. 5 show the side view of power set in second embodiment of the invention.
Specific embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation
Example is a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
In the description of the invention, it is necessary to explanation, term " " center ", " on ", D score, "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " interior ", " outward " be based on orientation shown in the drawings or position relationship, merely to
Be easy to the description present invention and simplify describe, rather than indicate imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.Additionally, term " first ", " second ",
" the 3rd " is only used for describing purpose, and it is not intended that indicating or implying relative importance.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Company ", " connection " should be interpreted broadly, for example, it may be being fixedly connected, or being detachably connected, or be integrally connected；Can
Being to mechanically connect, or electrically connect；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, Ke Yishi
Two connections of element internal.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this
Concrete meaning in invention.
With reference to shown in Fig. 1, micro grade includes micro-fluidic chip and power set than loading micro-fluidic chip system.
Micro-fluidic chip includes the first chip 1, the second chip 2 and the 3rd chip 3 that are sequentially overlapped up and down, the first chip 1
On offer the injection port 101 and outlet 102 for communicating up and down, offer microchannel 201 on the second chip 2.The He of injection port 101
Outlet 102 is respectively communicated with the two ends of microchannel 201.
In a preferred embodiment, injection port is provided with multiple, more preferably 3, designs different according to application demand
The injection port of number；Second chip is run through in microchannel up and down, and the 3rd chip is blank plate.
In other embodiments, microchannel recessed can also be formed at the lower surface of the first chip, the following table of the second chip
Face or the upper surface of the 3rd chip.
When microchannel is formed at the upper surface of the first chip lower surface or the second chip surface, the 3rd chip can not set
Put, or the 3rd chip is integrally formed with the second chip.
What be will be readily apparent is, it is also possible to set four layers of chip or more according to actual requirement, can be real in technical conditions
In the case of existing, it is also possible to which one layer of chip is only set.
Microchannel 201 includes mixed cell, can also according to the actual requirements be equipped with the functions such as sample-adding, reaction, filtering, detection
Unit.
Micro-fluidic chip material is not limited, and can be the materials such as glass, polymer, metal, silicon, and processing mode can be light
The methods such as quarter, numerical control, hot pressing, injection, are adjusted according to practical application request.
With reference to shown in Fig. 2, liquid storage unit 103 is formed between injection port 101 and microchannel 201.Injection port and liquid storage list
Unit 103 is opened on the first chip 1, and injection port 101 and liquid storage unit are communicated about 103.
In the technical scheme, an injection port back taper mouthful similar with the structure that liquid storage unit is formed, using machinery during loading
Arm or liquid-transfering gun add the target liq of designated volume, are stored in the liquid storage unit of bottom, and liquid storage unit volume can be with root
Factually border applied sample amount is adjusted.
The bottom of liquid storage unit is corresponding with the inlet of microchannel 201 together, so as in the presence of pressure, store up
Liquid in liquid unit can be entered into the MCA of the second chip.Microchannel inlet quantity is according to the individual of injection port
Number adjustment, each injection port corresponds to be communicated in an inlet respectively.Multiple liquid inlets are converged at one, and set a mixing
Unit, meets multiple liquid reactions and detection demand.Second chip can arbitrarily set the functions such as sample-adding, reaction, filtering, detection
Unit, meets reaction and the detection demand of different field.
Power set, are connected between injection port and power source, and major function is after to chip loading, there is provided corresponding
Power, mainly air pressure, it is also possible to which selection such as Piezoelectric Driving or ultrasonic wave drive other modes.
Power set are different according to type of drive and set, if pressure loading, dynamical system can be constant pressure pump or
The devices such as person's air compressor machine, and it is equipped with corresponding control system.If piezoelectricity loading or ultrasonic wave loading, dynamical system is then
Piezoelectric membrane or supersonic generator.
Embodiment 1
By taking air pressure as an example, there is provided one kind simply drives fixture to, realize that the micro liquid to micro-fluidic chip etc. compares loading.
With reference to shown in Fig. 2 and Fig. 3, power set include driving fixture to 4 and jointing 5, drive fixture to be located at the first core
Piece top, and face contacts between the first chip, jointing 4 is connected to power source, drives fixture to offer at least one on 4
Through hole 401, each through hole 401 corresponds to be communicated in an injection port 101 respectively, and jointing 4 is inserted in one end of through hole 401.
Further, the back-up ring 402 of annular is protruded out in through hole 401, is extruded between back-up ring 402 and micro-fluidic chip surface
There are packing ring 6, the opening inside diameter of the external diameter more than injection port 101 of packing ring 6.
Can realize driving fixture to the sealing between chip by packing ring 6.
In the technical scheme, because the first chip 1 needs and drives fixture to 4 sealings, so between there is seal washer 6,
It is larger in view of liquid measure on partially liq, so liquid storage unit 103 is relatively large in diameter, prevent packing ring 6 from dropping in liquid storage unit 103
In, so injection port and liquid storage unit be arranged into back taper mouth structure, but this case and back taper mouth structure is not limited to, as long as protecting
Card packing ring does not drop, amount of liquid storage is enough, can as needed change loading and liquid storage unit structure.
Drive fixture to be connected by screw, fixture, buckle, magnet or other pressuring methods with micro-fluidic chip,
So as to realize driving fixture to the sealing with chip.The top purpose of through hole 401 is and jointing is combined, jointing and source of the gas
Connection, so as to provide air pressure to injection port sealing, provides different air pressure, so as to realize difference by source of the gas to each injection port
Liquid enters microfluidic chip structure with different flow proportionals in injection port, so as to complete different experiment purposes.
Work is, during target liq added into chip loading and liquid storage unit by liquid-transfering gun or other instruments first,
Then will drive fixture to be fitted together with chip, be driven fixture to according to different, screw, fixture, buckle, magnet can be used
Or the connection of other pressuring methods, realize sealing.Now start power source, according to target loading ratio, to different liquid storage lists
Unit applies different pressure, integrally realizes the grade of micro liquid than loading, by the mixed structure on chip, realizes various
Waiting for micro liquid is carried out than loading and mixed synchronization simultaneously, and set on micro-fluidic chip different functional unit (sample-adding,
Reaction, filtering, detection), realize different application purposes.
Embodiment 2
In actual application, power set could be arranged to entirety, and agent structure is driven by mechanical arm, so as to realize
Change position.
With reference to shown in Fig. 4 and Fig. 5, the main structure body is hollow structure 601, and there is a projection 602 front end, is Liquid extracting
The pneumatic supply control for passing through upper end with release structure, the structure, realizes the extraction of target liq and is released in chip injection port
Put, then by pneumatic supply compressed gas, realize the loading of micro liquid and enter in chip object construction.
Structure leading portion is machined with gasket groove 603, and is provided with packing ring, when manipulator moves the structure, to structure
Apply pressure, realize the sealing of the structure and chip, realize the loading of liquid.The Liquid extracting and release structure of the structure front end
Can be clear, it is to avoid cross pollution, with practical value higher.
In sum, advantage of the present invention at least includes：
(1), trace sample loading, given full play to micro-fluidic chip reagent consumption it is few the characteristics of, it is to avoid tradition is with liquid
Push away the waste that the sample loading modes such as liquid are caused.
, etc. (2) than the easy structure of loading, liquid of traditional die etc. generally involves injecting pump structure than loading, this
Invention realizes the grade of various samples than loading and mixing by air pressure.
(3), simple to operate, the loading fixture of outfit is simple and practical, low cost, and can repeatedly use, with good
Commercial Prospect.
(4), flexibility is strong, and the structure of fixture and chip system is changeable, can according to the actual requirements change functional unit, real
Existing different detection targets.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
Pipe has been described in detail with reference to foregoing embodiments to the present invention, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, or which part or all technical characteristic are entered
Row equivalent；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (9)
1. a kind of micro grade is than loading micro-fluidic chip system, it is characterised in that including：
Micro-fluidic chip, including microchannel, multiple injection ports and at least one outlet, the injection port and outlet connect respectively
Pass through the two ends of the microchannel；
Power set, are connected between the injection port and power source, and the power source is driven using air pressure, Piezoelectric Driving or super
Sound wave drives.
2. micro grade according to claim 1 is than loading micro-fluidic chip system, it is characterised in that：The injection port and micro-
Liquid storage unit is formed between passage.
3. micro grade according to claim 2 is than loading micro-fluidic chip system, it is characterised in that：The power set bag
Include and drive fixture to and jointing, the jointing is connected to the power source, described to drive fixture to offer at least one
Individual through hole, each described through hole corresponds to be communicated in an injection port respectively, and the jointing is inserted in the through hole
One end.
4. micro grade according to claim 3 is than loading micro-fluidic chip system, it is characterised in that：Protruded out in the through hole
There is the back-up ring of annular, packing ring is extruded between the back-up ring and micro-fluidic chip surface, the external diameter of the packing ring enters more than described in
The opening inside diameter of sample mouthful.
5. micro grade according to claim 1 is than loading micro-fluidic chip system, it is characterised in that：The injection port it is interior
Footpath is less than the internal diameter of the liquid storage unit, and the liquid storage unit is communicated in the lower section of the injection port.
6. micro grade according to claim 1 is than loading micro-fluidic chip system, it is characterised in that：The power set tool
The one end for having a hollow cavity, the hollow cavity is connected to the power source, and the other end is communicated in the injection port, the power
Source is pneumatic supply, and the bottom of the hollow cavity protrudes out the Liquid extracting and release structure of back taper, the Liquid extracting and is released
Putting structure has the through hole for being connected with the injection port and hollow cavity respectively.
7. micro grade according to claim 6 is than loading micro-fluidic chip system, it is characterised in that：Power set and miniflow
Packing ring is provided between the contact surface for controlling chip.
8. micro grade according to claim 1 is than loading micro-fluidic chip system, it is characterised in that：The micro-fluidic chip
Including the first chip, the second chip and the 3rd chip that are sequentially overlapped up and down, the injection port and outlet are opened in described
On one chip, the microchannel is formed between second chip and the first chip, between the second chip and the 3rd chip or
Run through second chip up and down.
9. upper quadrat method of the micro grade than loading micro-fluidic chip system described in claim 6, it is characterised in that including：
Quantitative target liq is extracted and cached into hollow cavity by s1, power set under pneumatic supply suction function；
S2, the injection port that the Liquid extracting of hollow cavity bottom and release structure are aligned micro-fluidic chip；
S3, in the presence of pneumatic supply malleation, the target liq in hollow cavity is pushed into injection port；
S4, continuation pressure barotropic gas, are moved by the quantitative target liq of gas push in microchannel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710264363.XA CN106925359A (en) | 2017-04-21 | 2017-04-21 | Micro grade is than loading micro-fluidic chip system and upper quadrat method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710264363.XA CN106925359A (en) | 2017-04-21 | 2017-04-21 | Micro grade is than loading micro-fluidic chip system and upper quadrat method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109917147A (en) * | 2019-03-20 | 2019-06-21 | 武汉纺织大学 | A kind of automatic sampling apparatus and method |
| CN109985680A (en) * | 2019-04-24 | 2019-07-09 | 浙江警察学院 | Modular multichannel sample detection chip assembly suitable for hand-held SPR detector |
| CN112143784A (en) * | 2020-09-29 | 2020-12-29 | 生物岛实验室 | Space omics sequencing, single-cell apparent transcriptomics sequencing and positioning identification method |
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| CN1996014A (en) * | 2006-12-12 | 2007-07-11 | 中国人民解放军第二军医大学 | Array micro-fluidic chip device for use in drug metabolism screening |
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| CN114085897A (en) * | 2020-09-29 | 2022-02-25 | 生物岛实验室 | Space omics sequencing method |
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