CN115096855A - Multi-channel surface plasma resonance pesticide residue detection device and detection method - Google Patents
Multi-channel surface plasma resonance pesticide residue detection device and detection method Download PDFInfo
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- CN115096855A CN115096855A CN202210824250.1A CN202210824250A CN115096855A CN 115096855 A CN115096855 A CN 115096855A CN 202210824250 A CN202210824250 A CN 202210824250A CN 115096855 A CN115096855 A CN 115096855A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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Abstract
The invention discloses a multichannel surface plasma resonance pesticide residue detection device and a multichannel surface plasma resonance pesticide residue detection method, wherein in the detection device, a wall breaking assembly is arranged at the top of an exhaust assembly so as to extract all substances inside and outside the surface of fruit and vegetable skins, the exhaust assembly is in transmission connection with a driving device, and the exhaust assembly is used for floating fine bubbles mixed in a sample out of a liquid level in a mode of forming negative pressure by utilizing exhausted air, so that the problem that the SPR detection result is distorted due to the bubbles mixed in the sample is avoided. The driving device is in transmission connection with the output device, the driving device simultaneously drives the exhaust assembly and the output device to alternately operate, and the exhaust assembly is communicated with the wall breaking assembly due to the fact that the output device is communicated with the exhaust assembly, so that the sample solution is injected into the SPR detector while air bubbles are exhausted. The invention has the advantages of compact structure, stable operation, high system integration level and good portability, reduces the detection cost and is beneficial to the field detection of sources such as field ground and the like.
Description
Technical Field
The invention relates to the technical field of crop drug residue detection, in particular to a multichannel surface plasma resonance pesticide residue detection device and a multichannel surface plasma resonance pesticide residue detection method.
Background
Pesticide residues in vegetables and fruits can cause irreversible damage to human bodies, but the pesticide residues are inevitably used in agricultural product planting. The existing detection method of pesticide residues is a Surface Plasmon Resonance (SPR) detection method. The method has the characteristics of high speed, high sensitivity, no need of labeling, low cost and the like, is simple to operate, has the advantages of reusable chips and the like, but the current detection means needs to firstly pre-treat the sample, enrich, extract and concentrate the sample, and then can detect the sample, and is complex to operate. The invention designs a portable multi-channel SPR pesticide residue detection device which comprises an SPR detection unit, a pretreatment unit and a cleaning unit, so that agricultural products such as vegetables and fruits can be directly detected, the portable multi-channel SPR pesticide residue detection device can be used for field detection of sources such as field areas and the like, the process of collecting and conveying samples to a laboratory is avoided, pesticide residues can be analyzed and monitored in real time, and the pesticide dosage and the pesticide application time are guided.
The invention can directly detect agricultural products such as vegetables and fruits, can be used for field detection of sources such as field and the like, avoids the processes of sample collection and transportation to a laboratory, reduces the detection cost, and can analyze and monitor pesticide residues in real time without special technical personnel to guide the dosage and the application time.
Disclosure of Invention
Therefore, the invention provides a multi-channel surface plasma resonance pesticide residue detection device and a multi-channel surface plasma resonance pesticide residue detection method, and aims to solve the problem that the detection efficiency of pesticide residues is low due to the fact that pretreatment operation of samples is complex in the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
according to a first aspect of the present invention,
the invention provides a multichannel surface plasma resonance pesticide residue detection device which comprises a wall breaking assembly, an exhaust assembly, a driving device, an output device and an SPR detector, wherein the wall breaking assembly is arranged at the top of the exhaust assembly, the exhaust assembly is in transmission connection with the driving device, the driving device is in transmission connection with the output device, the output device is connected with the SPR detector, the output device is communicated with the exhaust assembly, and the exhaust assembly is communicated with the wall breaking assembly.
Further, the broken wall subassembly includes washing unit, broken wall mechanism, solenoid valve and power device, exhaust subassembly links to each other with the solenoid valve, the solenoid valve sets up in broken wall mechanism, the top and the washing unit of broken wall mechanism are articulated, washing unit is connected with the power device transmission, power device sets up in the bottom of broken wall mechanism and is connected with broken wall mechanism transmission.
Further, the flushing device comprises a turnover cover and peristaltic pumps, the top of the wall breaking mechanism is hinged with the turnover cover, and a plurality of peristaltic pumps are arranged in the turnover cover;
the peristaltic pump includes pump head, pump line, lower pump head and driver, power device is connected with the driver transmission, the driver rotates to set up under on the pump head, just the driver extrusion pump line warp, the pump line sets up under between pump head and the last pump head, pump head and last pump head are fixed to be set up in flip down.
Further, the wall breaking mechanism comprises a centrifugal filter barrel, a rotary knife rest rod, a wall breaking inner cavity and a feeding member, the driver is in coaxial transmission connection with the rotary knife rest rod, the rotary knife rest rod is located at the axis of the centrifugal filter barrel, the centrifugal filter barrel is located in the wall breaking inner cavity, and the wall breaking inner cavity is arranged in the feeding member;
the rotary cutter rest rod comprises a spline shaft, a cutter rest and a gear shaft, the spline shaft is inserted in the driver, the spline shaft is connected with one end of the cutter rest, the other end of the cutter rest is connected with the gear shaft, and the gear shaft is in transmission connection with the power device.
Furthermore, the power device comprises a stepping motor, a coaxial reversal mechanism and a gear box, wherein the gear shaft is inserted on the coaxial reversal mechanism, the coaxial reversal mechanism is in transmission connection with the centrifugal filter barrel, the coaxial reversal mechanism is arranged in the gear box, and the gear box is internally provided with the stepping motor which is in power connection with the coaxial reversal mechanism.
Further, the exhaust assembly comprises a liquid discharge pipe, a negative pressure pipe, an exhaust device and a piston rod, the electromagnetic valve is communicated with the top of the negative pressure pipe, the bottom of the negative pressure pipe is communicated with one end of the liquid discharge pipe, the side face of the negative pressure pipe is communicated with the exhaust device, and the piston rod is arranged in the exhaust device in a sliding mode.
Further, exhaust apparatus is including arranging cylinder, gas vent, air inlet, inner chamber and closing cap even, piston rod activity is inserted and is established at the inner chamber, the inner chamber sets up in arranging cylinder even and inner chamber quantity is a plurality of, arbitrary one the inner chamber all communicates with a set of gas vent and air inlet, the gas vent all sets up the check valve.
Furthermore, the output device comprises a filter, a liquid inlet, a continuous-discharge pump body and plunger rods, the other end of the liquid discharge pipe is communicated with the liquid inlet, the liquid inlet is communicated with the continuous-discharge pump body, the plunger rods are movably arranged on one side of the continuous-discharge pump body, and the other side of the continuous-discharge pump body is connected with the filter in parallel.
Furthermore, the driving device comprises a servo motor, a crankshaft mechanism and a shell, the sealing cover is screwed and fixed on the top of the shell, the servo motor is arranged on the side surface of the shell, and the servo motor is in transmission connection with the crankshaft mechanism;
the crankshaft mechanism comprises a plunger connecting rod, a crankshaft and a piston connecting rod, the servo motor is in coaxial transmission connection with the crankshaft, the plunger connecting rod and the piston connecting rod are sleeved on the crankshaft, the plunger connecting rod is hinged with the plunger rod, and the piston connecting rod is hinged with the piston rod.
According to a second aspect of the present invention,
the invention provides a multi-channel surface plasma resonance pesticide residue detection device, a multi-channel surface plasma resonance pesticide residue detection method, which applies the multi-channel surface plasma resonance pesticide residue detection device and comprises the following steps;
s1, putting a sample into a wall breaking mechanism, closing a flip cover, starting a stepping motor, driving a rotary knife rest rod and a centrifugal filter barrel to coaxially rotate reversely, and simultaneously driving a peristaltic pump to inject distilled water to dilute the sample;
s2, communicating the filter with an interface cover of the SPR detector through a conduit, and then tensioning and clamping the shell and the interface cover by utilizing a lock catch;
s3, starting a servo motor, wherein the servo motor drives a crankshaft mechanism to move, so that a plunger rod is driven to inject a sample into a test slot of the SPR detector for detection;
and S4, connecting the peristaltic pump into distilled water, cleaning residues of the original sample by using idle equipment, and then introducing a detergent to elute pesticide residues in the test slot.
The invention has the following advantages:
the technical scheme provided by the invention mainly integrates the detection process of pesticide residues, so that the detection process can be used together with an SPR detector, and the detection device comprises an SPR detection unit, a pretreatment unit and a cleaning unit, so that the complex process of sample collection and transportation to a laboratory is simplified, the detection cost is reduced, the design can be used for directly detecting agricultural products such as vegetables and fruits, can be used for field site detection of sources such as fields, can be used for analyzing and monitoring pesticide residues in real time without special technical personnel, and can guide the pesticide dosage and the pesticide application time, and the problem of low pesticide detection efficiency caused by complex pretreatment operation of samples in the prior art is solved.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so that those skilled in the art can understand and read the present invention, and do not limit the conditions under which the present invention can be implemented, so that the present invention has no technical significance, and any modifications of the structures, changes of the ratio relationships, or adjustments of the sizes, without affecting the efficacy and the purpose of the present invention, should still fall within the scope of the technical contents disclosed in the present invention.
FIG. 1 is a perspective view of a multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 2 is a perspective view of an SPR detector of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 3 is a perspective view of an output device of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 4 is a perspective view of a crankshaft mechanism of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 5 is a perspective view of a driving device of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 6 is a perspective view of an exhaust device of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 7 is a perspective view of an exhaust assembly of the multi-channel surface plasmon resonance pesticide residue detection apparatus provided by the invention;
FIG. 8 is a perspective view of a power device of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 9 is a perspective view of a wall breaking mechanism of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 10 is a perspective view of a flushing device of the multi-channel surface plasmon resonance pesticide residue detection device provided by the invention;
FIG. 11 is a perspective view of a wall breaking assembly of the multi-channel surface plasmon resonance pesticide residue detection apparatus provided by the present invention;
in the figure: 1, breaking a wall of a component; 11 a flushing device; 111 a flip cover; 112, an upper pump head; 113 a pump tube; 114 a lower pump head; 115 drivers; 12 a wall breaking mechanism; 121 centrifugal filter vat; 122 rotating the cutter bar; 1221 a spline shaft; 1222 a tool holder; 1223 a gear shaft; 123 breaking the inner cavity; 124 a feeding member; 13 electromagnetic valve; 14 a power plant; 141 a stepping motor; 142 coaxial inversion mechanism; 143 a gear case; 2, an exhaust component; 21 a drain pipe; 22 a negative pressure tube; 23 an exhaust device; 231 a tandem cylinder; 232 an exhaust port; 233 air inlet; 234 lumen; 235 sealing the cover; 24 a piston rod; 3 a driving device; 31 a servo motor; a 32-crankshaft mechanism; 321 plunger connecting rods; 322 a crankshaft; 323 a piston rod; 33 a housing; 4 an output device; 41 a filter; 42 liquid inlet; 43 continuous-row pump body; 44 a plunger rod; 5 SPR detector; 51 a housing; 52 locking and buckling; 53 test slots; 54 access the cover.
Detailed Description
The embodiments of the present invention are described below in terms of specific embodiments, and other advantages and effects of the invention will be readily apparent to those skilled in the art from the disclosure of the present specification. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
As shown in fig. 1-11, the invention provides a multi-channel surface plasmon resonance pesticide residue detection device, which comprises a wall breaking component 1, an exhaust component 2, a driving device 3, an output device 4 and an SPR detector 5, wherein the wall breaking component 1 is arranged at the top of the exhaust component 2 and is used for crushing fruits and vegetables serving as samples so as to extract all substances inside and outside the epidermis. As shown in fig. 1, the exhaust assembly 2 is in transmission connection with the driving device 3, and the exhaust assembly 2 is used for floating fine bubbles mixed in the sample out of the liquid level by using a mode of forming negative pressure by using exhaust air, so that the problem of distortion of a test result of the SPR detector 5 caused by bubbles mixed in the sample is avoided. Drive arrangement 3 is connected with output device 4 transmission, and drive arrangement 3 drives exhaust subassembly 2 and output device 4 alternate operation simultaneously, because output device 4 and exhaust subassembly 2 intercommunication, exhaust subassembly 2 and broken wall subassembly 1 intercommunication, inject sample solution into SPR detector 5 when discharging the bubble from this.
According to one embodiment of the present disclosure, the wall-breaking assembly 1 includes a washing device 11, a wall-breaking mechanism 12, an electromagnetic valve 13 and a power device 14, as shown in fig. 11, the exhaust assembly 2 is connected to the electromagnetic valve 13, the electromagnetic valve 13 is automatically opened at intervals, and the electromagnetic valve 13 is disposed on the wall-breaking mechanism 12, so as to control the amount of the sample solution entering the exhaust assembly 2. The top of broken wall mechanism 12 is articulated with washing unit 11, and washing unit 11 is connected with power device 14 transmission, and power device 14 sets up in the bottom of broken wall mechanism 12 and is connected with broken wall mechanism 12 transmission. The power device 14 drives the flushing device 11 and the wall breaking mechanism 12 to operate simultaneously, so that the flushing device 11 is used for injecting distilled water to dilute the sample while the fruit and vegetable sample is crushed, and the residual sample is effectively and completely eluted from the device.
According to one embodiment of the present disclosure, the flushing device 11 includes a flip cover 111 and a peristaltic pump, the top of the wall breaking mechanism 12 is hinged to the flip cover 111, and the flip cover 111 is provided with a plurality of peristaltic pumps therein. As shown in fig. 11 and 10, when in use, the flip cover 111 is closed, so that the power device 14 drives the peristaltic pump to operate and inject distilled water into the wall-breaking inner cavity 123, and further, in order to clean pesticide residues in the SPR detector 5, after the sample is cleaned with distilled water, the reagent solution is injected by the peristaltic pump to elute, so that the SPR detector 5 is reused. The peristaltic pump comprises an upper pump head 112, a pump tube 113, a lower pump head 114 and a driver 115, wherein the power device 14 is in transmission connection with the driver 115, as shown in fig. 10, the driver 115 is rotatably arranged on the lower pump head 114, actually, the driver 115 is a turntable inserted in the lower pump head 114, when the driver 115 rotates, the pump tube 113 can be continuously extruded to repeatedly deform, the pump tube 113 is a rubber hose and is arranged between the lower pump head 114 and the upper pump head 112, the upper pump head 112 inputs distilled water, and the lower pump head 114 outputs the distilled water to the centrifugal filter barrel 121. The lower pump head 114 and the upper pump head 112 are fixedly disposed in the flip cover 111, and when the flip cover 111 is closed, the rotary knife rack bar 122 can be inserted into the driver 115, so as to drive the peristaltic pump to operate.
According to an embodiment of the present disclosure, the wall breaking mechanism 12 includes a centrifugal filter drum 121, a rotary knife rack bar 122, a wall breaking cavity 123 and a feeding member 124, and the driver 115 is coaxially and drivingly connected to the rotary knife rack bar 122. As shown in fig. 9, the wall-breaking inner cavity 123 is disposed in the feeding member 124, the feeding member 124 is a four-connected-row cylinder, the rotary knife-bar support 122 is located at the axis of the centrifugal filter vat 121, the centrifugal filter vat 121 is located in the wall-breaking inner cavity 123, and the rotary knife-bar support 122 and the centrifugal filter vat 121 are both in transmission connection with the coaxial inversion mechanism 142, so as to drive the rotary knife-bar support 122 and the centrifugal filter vat 121 to rotate in the opposite direction, thereby improving the crushing effect of the knife rest 1222. The rotary cutter support rod 122 is rotated to crush the fruits and vegetables to extract juice, and the centrifugal filter bucket 121 is rotated to primarily filter the residue formed by crushing the fruits and vegetables. Simultaneously, further centrifugal filter vat 121 washs through the centrifugal force that forms and combine the distilled water, can draw out most of the material in the fruit vegetables, improves the precision of test from heat.
According to an embodiment of the present disclosure, the rotary cutter holder bar 122 includes a spline shaft 1221, a cutter holder 1222, and a gear shaft 1223, the spline shaft 1221 is integrally connected to one end of the cutter holder 1222, and the other end of the cutter holder 1222 is integrally connected to the gear shaft 1223, thereby enabling the spline shaft 1221 to coaxially rotate with the cutter holder 1222 and the gear shaft 1223. The gear shaft 1223 is in transmission connection with the coaxial reversing mechanism 142, so that the coaxial reversing mechanism 142 can drive the spline shaft 1221 and the tool rest 1222 to rotate synchronously, and the spline shaft 1221 is inserted into the driver 115, so that the peristaltic pump is driven to operate together, and distilled water is continuously injected into the wall-broken inner cavity 123 to perform the functions of cleaning, diluting and promoting dissociation of antigen and antibody.
According to an embodiment of the present disclosure, the power device 14 includes a stepping motor 141, a coaxial reversing mechanism 142, and a gear box 143, the gear shaft 1223 is inserted on the coaxial reversing mechanism 142, the coaxial reversing mechanism 142 is in transmission connection with the centrifugal filter drum 121, the coaxial reversing mechanism 142 is disposed in the gear box 143, and the stepping motor 141 in power connection with the coaxial reversing mechanism 142 is disposed in the gear box 143. As shown in fig. 8, the coaxial reversing mechanism 142 is composed of four bevel gears symmetrically arranged two by two, the bevel gears arranged up and down have the same structure and the same rotation speed, but the rotation directions are opposite, the centrifugal filter vat 121 is coaxially and drivingly connected with the bevel gear arranged above, the gear shaft 1223 is coaxially and drivingly connected with the bevel gear arranged below, and the bevel gear arranged on the side is coaxially and drivingly connected with the stepping motor 141 for outputting a power torque, so that the technical effect of synchronously rotating the centrifugal filter vat 121 and the tool rest 1222 can be achieved.
According to one embodiment of the present disclosure, the exhaust assembly 2 includes a liquid discharge pipe 21, a negative pressure pipe 22, an exhaust device 23, and a piston rod 24, the electromagnetic valve 13 is communicated with the top of the negative pressure pipe 22, the electromagnetic valve 13 is opened at a fixed time, a small amount of sample solution is input into the negative pressure pipe 22, and then the piston rod 24 drives the exhaust device 23 to exhaust air in the negative pressure pipe 22, so as to form a negative pressure in the negative pressure pipe 22, and further to enable micro bubbles in the sample to float out of the liquid surface and break, thereby achieving the purpose of discharging bubbles in the sample solution. As shown in fig. 7, the bottom of the negative pressure tube 22 communicates with one end of the drain tube 21, the side surface of the negative pressure tube 22 communicates with the exhaust unit 23, and the piston rod 24 is slidably provided in the exhaust unit 23. Because the piston rod 24 and the plunger rod 44 move alternately, after the negative pressure tube 22 is subjected to negative pressure vacuum treatment, the sample solution in the negative pressure tube 22 enters the output device 4, and the sample solution can continuously enter the SPR detector 5 along with the continuous operation of the driving device 3, so that the detection work is completed.
According to one embodiment of the disclosure, the exhaust device 23 includes a continuous air cylinder 231, an exhaust port 232, an air inlet 233, an inner cavity 234 and a sealing cover 235, the piston rod 24 is movably inserted in the inner cavity 234, the inner cavity 234 is disposed in the continuous air cylinder 231, the number of the inner cavities 234 is several, any one of the inner cavities 234 is communicated with a group of the exhaust ports 232 and the air inlet 233, and the exhaust ports 232 are all provided with one-way valves. As shown in fig. 6, the inner cavity 234 communicates with the negative pressure tube 22 through the air inlet 233, and as the piston rod 24 continuously fluctuates, the air pressure in the inner cavity 234 and the negative pressure tube 22 repeatedly increases and decreases, so that the micro-bubbles in the sample solution are forced to float above the liquid level and burst, and the air generated by the burst bubbles is discharged from the air outlet 232 along with the one-way valve.
According to a specific embodiment disclosed by the invention, the output device 4 comprises a filter 41, a liquid inlet 42, a continuous-discharge pump body 43 and plunger rods 44, the other end of the liquid discharge pipe 21 is communicated with the liquid inlet 42, the liquid inlet 42 is communicated with the continuous-discharge pump body 43, a plurality of plunger rods 44 are movably arranged on one side of the continuous-discharge pump body 43, and the other side of the continuous-discharge pump body 43 is connected with the plurality of filters 41 in parallel. The filter 41 removes impurities from the sample solution for the second time. As shown in FIG. 3, the plunger rod 44 moves telescopically in the continuous pump body 43, and the sample solution is continuously output for detection by changing the volume.
According to an embodiment of the present disclosure, the driving device 3 includes a servo motor 31, a crank mechanism 32 and a housing 33, the sealing cover 235 is screwed on the top of the housing 33, the servo motor 31 is disposed on the side of the housing 33, the servo motor 31 is in transmission connection with the crank mechanism 32 to provide power for the crank mechanism 32, as shown in fig. 4 and 5, the tandem pump body 43 is disposed orthogonally to the tandem cylinder 231. The crank mechanism 32 comprises a plunger connecting rod 321, a crank shaft 322 and a piston connecting rod 323, the servo motor 31 is in coaxial transmission connection with the crank shaft 322, the plunger connecting rod 321 and the piston connecting rod 323 are sleeved on the crank shaft 322, the plunger connecting rod 321 is hinged with the plunger rod 44, and the piston connecting rod 323 is hinged with the piston rod 24, so that the plunger connecting rod 321 and the piston connecting rod 323 move alternately when the crank shaft 322 rotates, the exhaust and output processes of the sample solution are independent and do not interfere with each other, the device can operate stably, and the reliability of the device in operation is improved.
Example 2
The invention discloses a method for detecting multi-channel surface plasma resonance pesticide residues, which applies the device for detecting multi-channel surface plasma resonance pesticide residues and comprises the following steps;
s1, putting a sample into a wall breaking mechanism 12, closing a turnover cover 111, starting a stepping motor 141, driving a rotary knife rest rod 122 and a centrifugal filter barrel 121 to coaxially rotate reversely, and simultaneously driving a peristaltic pump to inject distilled water to dilute the sample;
s2, the filter 41 is communicated with an interface cover 54 of the SPR detector 5 through a conduit, and then the shell 51 and the interface cover 54 are tightly fastened and clamped by using the lock catch 52;
s3, starting the servo motor 31, wherein the servo motor 31 drives the crankshaft mechanism 32 to move, so that the plunger rod 44 is driven to inject the sample into the test slot 53 of the SPR detector 5 for detection;
s4, enabling the peristaltic pump to be connected into distilled water, enabling the idling equipment to clean the original sample, and then enabling the detergent to be introduced to elute pesticide residues in the test groove.
According to an embodiment of the present disclosure, the SPR detector 5 includes a housing 51, a latch 52, a test slot 53 and a mouthpiece 54, a biochip is disposed in the test slot 53, the biochip contains an antibody, a compound forming a covalent bond through an antigen and an antibody reaction is used, the pesticide residue content can be detected by applying surface plasmon resonance, and the antigen and antibody combination is reversible after forming a complex, so that the antigen and antibody can be dissociated after a detergent is introduced for reuse of the biochip, as shown in fig. 2, finally, the detected sample solution passes through the test slot 53 and enters a recovery tank.
The application process of the embodiment of the invention is as follows:
the invention relates to a multichannel surface plasma resonance pesticide residue detection device and a multichannel surface plasma resonance pesticide residue detection method, when the multichannel surface plasma resonance pesticide residue detection device is used, firstly, fruit and vegetable products with unit weight are put into a centrifugal filter barrel 121, a turning cover 111 is closed, then an upper pump head 112 is communicated with distilled water, a power device 14 is started to enable a stepping motor 141 to operate, after a period of time, a servo motor 31 is started, after detection is finished, residues in the centrifugal filter barrel 121 and a filter 41 are taken out, finally, equipment is operated in an idling mode until the distilled water washes the original sample solution clean, and then a cleaning agent is introduced to enable antigen antibodies of a biochip to be completely dissociated.
Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Accordingly, it is intended that all such modifications and alterations be included within the scope of this invention as defined in the appended claims.
Claims (10)
1. The utility model provides a incomplete detection device of multichannel surface plasma resonance farming, its characterized in that, includes broken wall subassembly (1), exhaust subassembly (2), drive arrangement (3), output device (4) and SPR detector (5), broken wall subassembly (1) sets up the top at exhaust subassembly (2), exhaust subassembly (2) are connected with drive arrangement (3) transmission, drive arrangement (3) are connected with output device (4) transmission, output device (4) link to each other with SPR detector (5), output device (4) and exhaust subassembly (2) intercommunication, exhaust subassembly (2) and broken wall subassembly (1) intercommunication.
2. The multi-channel surface plasmon resonance pesticide residue detection device as claimed in claim 1, wherein the wall breaking assembly (1) comprises a flushing device (11), a wall breaking mechanism (12), an electromagnetic valve (13) and a power device (14), the exhaust assembly (2) is connected with the electromagnetic valve (13), the electromagnetic valve (13) is arranged on the wall breaking mechanism (12), the top of the wall breaking mechanism (12) is hinged with the flushing device (11), the flushing device (11) is in transmission connection with the power device (14), and the power device (14) is arranged at the bottom of the wall breaking mechanism (12) and is in transmission connection with the wall breaking mechanism (12).
3. The multi-channel surface plasmon resonance pesticide residue detection device as claimed in claim 2, wherein the flushing device (11) comprises a flip cover (111) and a peristaltic pump, the top of the wall breaking mechanism (12) is hinged with the flip cover (111), and a plurality of peristaltic pumps are arranged in the flip cover (111);
the peristaltic pump includes pump head (112), pump line (113), lower pump head (114) and driver (115), power device (14) are connected with driver (115) transmission, driver (115) rotate to be set up under on pump head (114), just driver (115) extrusion pump line (113) warp, pump line (113) set up under between pump head (114) and last pump head (112), pump head (114) and last pump head (112) are fixed to be set up in flip (111) down.
4. The multi-channel surface plasmon resonance pesticide residue detection device as claimed in claim 3, wherein the wall breaking mechanism (12) comprises a centrifugal filter barrel (121), a rotating knife rest rod (122), a wall breaking inner cavity (123) and a feeding member (124), the driver (115) is in coaxial transmission connection with the rotating knife rest rod (122), the rotating knife rest rod (122) is located at the axis of the centrifugal filter barrel (121), the centrifugal filter barrel (121) is located in the wall breaking inner cavity (123), and the wall breaking inner cavity (123) is arranged in the feeding member (124);
the rotary cutter rest rod (122) comprises a spline shaft (1221), a cutter rest (1222) and a gear shaft (1223), the spline shaft (1221) is inserted into the driver (115), the spline shaft (1221) is connected with one end of the cutter rest (1222), the other end of the cutter rest (1222) is connected with the gear shaft (1223), and the gear shaft (1223) is in transmission connection with the power device (14).
5. The multi-channel surface plasmon resonance pesticide residue detection device as claimed in claim 4, wherein the power device (14) comprises a stepping motor (141), a coaxial reversing mechanism (142) and a gear box (143), the gear shaft (1223) is inserted on the coaxial reversing mechanism (142), the coaxial reversing mechanism (142) is in transmission connection with the centrifugal filter barrel (121), the coaxial reversing mechanism (142) is arranged in the gear box (143), and the stepping motor (141) in power connection with the coaxial reversing mechanism (142) is arranged in the gear box (143).
6. The multichannel surface plasmon resonance pesticide residue detection device as claimed in claim 5, wherein the exhaust assembly (2) comprises a liquid discharge pipe (21), a negative pressure pipe (22), an exhaust device (23) and a piston rod (24), the electromagnetic valve (13) is communicated with the top of the negative pressure pipe (22), the bottom of the negative pressure pipe (22) is communicated with one end of the liquid discharge pipe (21), the side surface of the negative pressure pipe (22) is communicated with the exhaust device (23), and the piston rod (24) is slidably arranged in the exhaust device (23).
7. The multi-channel surface plasma resonance pesticide residue detection device as recited in claim 6, wherein the exhaust device (23) comprises a continuous exhaust cylinder (231), exhaust ports (232), air inlets (233), inner cavities (234) and a sealing cover (235), the piston rods (24) are movably inserted into the inner cavities (234), the inner cavities (234) are arranged in the continuous exhaust cylinder (231) and the number of the inner cavities (234) is several, any one of the inner cavities (234) is communicated with one group of the exhaust ports (232) and the air inlets (233), and the exhaust ports (232) are provided with one-way valves.
8. The multi-channel surface plasmon resonance pesticide residue detection device as claimed in claim 7, wherein the output device (4) comprises a filter (41), a liquid inlet (42), a continuous-discharge pump body (43) and plunger rods (44), the other end of the liquid discharge pipe (21) is communicated with the liquid inlet (42), the liquid inlet (42) is communicated with the continuous-discharge pump body (43), one side of the continuous-discharge pump body (43) is movably provided with a plurality of plunger rods (44), and the other side of the continuous-discharge pump body (43) is connected with a plurality of filters (41) in parallel.
9. The multi-channel surface plasma resonance pesticide residue detection device as claimed in claim 8, wherein the driving device (3) comprises a servo motor (31), a crankshaft mechanism (32) and a housing (33), the sealing cover (235) is screwed and fixed on the top of the housing (33), the servo motor (31) is arranged on the side surface of the housing (33), and the servo motor (31) is in transmission connection with the crankshaft mechanism (32);
the crank mechanism (32) comprises a plunger connecting rod (321), a crankshaft (322) and a piston connecting rod (323), the servo motor (31) is in coaxial transmission connection with the crankshaft (322), the crankshaft (322) is sleeved with the plunger connecting rod (321) and the piston connecting rod (323), the plunger connecting rod (321) is hinged with the plunger rod (44), and the piston connecting rod (323) is hinged with the piston rod (24).
10. A method for detecting multi-channel surface plasma resonance pesticide residues, which is characterized in that the multi-channel surface plasma resonance pesticide residue detection device provided by claim 9 is applied, and comprises the following steps;
s1, putting a sample into a wall breaking mechanism (12), closing a flip cover (111), starting a stepping motor (141), driving a rotary cutter bar (122) and a centrifugal filter barrel (121) to coaxially rotate reversely, and simultaneously driving a peristaltic pump to inject distilled water to dilute the sample;
s2, the filter (41) is communicated with an interface cover (54) of the SPR detector (5) through a conduit, and then the shell (51) and the interface cover (54) are tensioned and clamped by a lock catch (52);
s3, starting the servo motor (31), wherein the servo motor (31) drives the crankshaft mechanism (32) to move, so that the plunger rod (44) is driven to inject a sample into a test slot (53) of the SPR detector (5) for detection;
s4, enabling the peristaltic pump to be connected into distilled water, enabling the idling equipment to clean residues of the original sample, and then enabling the detergent to be introduced to elute pesticide residues in the test groove.
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