CN112834464A

CN112834464A - Sample introduction system suitable for multi-channel terminal reflection type optical fiber SPR sensor and use method thereof

Info

Publication number: CN112834464A
Application number: CN202110199470.5A
Authority: CN
Inventors: 丁利; 清江; 李明安; 王季诚
Original assignee: NINGBO HEIGER ELECTRICAL APPLIANCES CO Ltd
Current assignee: NINGBO HEIGER ELECTRICAL APPLIANCES CO Ltd
Priority date: 2021-02-22
Filing date: 2021-02-22
Publication date: 2021-05-25

Abstract

The invention discloses a sample introduction system suitable for a multi-channel terminal reflection type optical fiber SPR sensor, which comprises: the device comprises a shell, a sample injection mechanism, an optical fiber SPR detection mechanism and a liquid discharge mechanism, wherein the sample injection mechanism is arranged in the shell and used for collecting and conveying a sample to be detected, the optical fiber SPR detection mechanism is used for detecting the sample to be detected and is positioned at a terminal reflection type optical fiber SPR detection end, the liquid discharge mechanism is used for self-cleaning and discharging detection waste liquid, the sample injection mechanism is arranged at the front side sample injection end of the optical fiber SPR detection mechanism, and the liquid discharge mechanism is arranged at the rear side sample outlet end of. The optical fiber channels connected in parallel are distributed on the same plane along the circumference at equal angles, the lengths of sample flow paths of each internal liquid path are equal, the dead volume is small, at least two optical fiber sensors are arranged in each optical fiber channel, the same sample can be sampled to be detected simultaneously or multiple samples can be sampled to be detected, the detection data of multiple groups of optical fiber sensors are detected, each internal liquid path is relatively independent and has parallelism, the consumption of the sample is reduced, and the detection efficiency is improved.

Description

Sample introduction system suitable for multi-channel terminal reflection type optical fiber SPR sensor and use method thereof

Technical Field

The invention belongs to the technical field of SPR (surface plasmon resonance) biosensing detectors, and particularly relates to a sample feeding system suitable for a multi-channel terminal reflection type optical fiber SPR sensor.

Background

Fiber Surface Plasmon Resonance (SPR) sensors are the product of an organic combination of high sensitivity surface plasmon resonance sensor technology with low energy consumption fiber transmission technology. The optical fiber has the advantages of being flexible, electrically insulating, corrosion resistant, non-heating and non-radiation when in use, and capable of working under complex environmental conditions of strong electromagnetic interference, inflammability, explosion and toxic gases. When light enters the light sparse medium from the light dense medium, the light is totally reflected at the interface of the two media, and the electromagnetic field intensity of the light wave is not immediately reduced to zero at the interface, but is exponentially attenuated along with the incident depth to form evanescent waves. The effective depth of the evanescent wave is generally 100-200nm, and the evanescent wave still acts at the interface between the metal film and the solution or air because the thickness of the metal film of the light SPR sensor is smaller than the depth of the evanescent wave. Meanwhile, at the interface of the metal film and the sample, free electrons on the surface of the metal are excited to form a surface plasma wave. When the wave vector of the evanescent wave is equal to the wave vector of the surface plasma wave, the reflectivity is minimum. The device can make sensitive response to the tiny change of the composition of the medium to be measured on the surface of the sensor, is suitable for researching the biological and chemical reactions between the substances in the sensitive layer of the surface of the sensor and the medium solution, further quantitatively measuring the trace biological and chemical active substances in the medium solution, has strong anti-electromagnetic interference capability, and is widely applied to the fields of biology, medicine, environmental protection, food safety, analytical chemistry and the like.

Fiber SPR replaces the traditional prism structure with specially treated fiber, usually using wavelength modulation principles. The terminal reflection type SPR is similar to a probe structure, and the resonance of two sides of a side metal film area is realized by plating a reflection film on the end face of the optical fiber and plating a metal film (generally a gold film) on the side face of the optical fiber, so that the sensitivity is further improved. Compared with the traditional prism structure SPR detection system, the optical fiber SPR detection system has the advantages of simple structure, small volume and low cost, and is more suitable for field detection.

In actual detection, direct requirements are often placed on the number of detection samples and items, and many interference factors affect the detection result, so that repeated tests are needed, and the average value is obtained after the noise background is subtracted, so that a result with higher accuracy is obtained. At present, the traditional spectrum modulation type SPR sensor system only can realize a single detection channel, is difficult to meet the requirement of detecting a plurality of samples at one time, and has low detection efficiency.

Therefore, in order to solve the above technical problems, a multi-channel sample feeding system is needed to be designed to realize simultaneous and parallel detection of multiple targets or simultaneous and repeated detection of the same target substance.

Disclosure of Invention

The invention aims to provide a sample feeding system which is simple in structure and operation, reduces the sample consumption of a reflective terminal type optical fiber SPR and improves the detection efficiency, and is suitable for a multi-channel terminal reflective optical fiber SPR sensor.

The technical scheme of the invention is as follows:

a sample introduction system suitable for a multi-channel terminal reflection type optical fiber SPR sensor comprises a shell, a sample introduction mechanism, an optical fiber SPR detection mechanism and a liquid discharge mechanism, wherein the sample introduction mechanism is arranged in the shell and used for collecting and conveying a sample to be detected, the optical fiber SPR detection mechanism is used for detecting the sample to be detected and is positioned at a terminal reflection type optical fiber SPR detection end, the liquid discharge mechanism is used for self-cleaning and discharging detection waste liquid, the sample introduction mechanism is arranged at the front side sample introduction end of the optical fiber SPR detection mechanism, the liquid discharge mechanism is arranged at the rear side sample discharge end of the optical fiber SPR detection mechanism, and the sample introduction mechanism, the optical fiber SPR detection mechanism;

the sampling mechanism comprises a sample tube, a sampling needle, a peristaltic pump and a liquid discharge station, wherein the sample tube is used for containing a sample to be detected, the sampling needle is used for collecting the sample to be detected in the sample tube, the peristaltic pump is used for pumping the sample to be detected and pumping out detection waste liquid, the liquid discharge station is used for receiving the detection waste liquid, the sample tube is arranged at the front side sample injection end of the optical fiber SPR detection mechanism, the sampling needle is positioned above the sample tube, the sampling needle corresponds to the sample tube to collect the sample to be detected, the liquid discharge station is arranged at the rear side sample discharge end of the optical fiber SPR detection mechanism, the sampling needle collects the sample to be detected and pumps the sample to be detected into the optical fiber SPR detection mechanism under the action of the peristaltic pump, and after detection, the detection waste liquid generated by the;

the optical fiber SPR detection mechanism is composed of at least four optical fiber channels connected in parallel, a liquid inlet and a liquid outlet are formed at two ends of each optical fiber channel respectively, an internal liquid path is formed in each optical fiber channel, each internal liquid path is relatively independent and connected in parallel, a sample to be detected in each sampling needle is input into the optical fiber channel from the liquid inlet and is output from the liquid outlet through the internal liquid path through a capillary tube, so that a plurality of optical fiber channels simultaneously and parallelly collect a plurality of samples to be detected, at least two optical fiber sensors which are sequentially connected in series are installed in the internal liquid path of each optical fiber channel to form multi-channel and multi-path detection, wherein a bottom plate is installed in each optical fiber channel, a connecting seat is fixedly installed on the bottom plate, a liquid inlet interface and a liquid outlet interface are formed in the top of the connecting seat, and the capillary tube is input through the liquid, the optical fiber sensor is characterized in that an optical fiber is externally connected with the connecting seat, a sensor inserting opening is formed in one side of the connecting seat, each optical fiber sensor is installed in the sensor inserting opening through an installation connector, and the optical fiber is inserted into the optical fiber sensor to be used for detecting a sample to be detected in an internal liquid path;

the draining mechanism comprises a buffer bottle communicated through a capillary tube and used for storing buffer liquid, a valve station used for switching the flow direction of liquid in the capillary tube, a diaphragm pump used for generating positive pressure or negative pressure, a plunger pump used for pumping quantitative samples to be detected or buffer liquid into a sampling needle, a vacuum cup used for providing pressure in cooperation with the diaphragm pump, a waste liquid cup used for receiving cleaning waste liquid and a waste liquid bottle used for collecting the cleaning waste liquid and detecting the waste liquid, the buffer liquid in the buffer bottle is pumped into the sampling needle under the action of the plunger pump for cleaning, the cleaning waste liquid after cleaning is discharged into the waste liquid cup under the action of the valve station, the diaphragm pump and the vacuum cup and is finally discharged into the waste liquid bottle for collection.

In the above technical scheme, capillary connectors are installed on the liquid inlet interface and the liquid outlet interface of the connecting seat so as to be connected with the capillary.

In the above technical scheme, the sampling needle passes through actuating mechanism and installs on the casing, actuating mechanism includes horizontal migration step motor and vertical movement step motor and removes in order to be used for controlling the position of sampling needle, makes the sampling needle remove to required sample cell and goes out the sample that gathers to be measured.

In the above technical scheme, advance kind mechanism and include sampling platform and sample holder, sample holder horizontal installation is on the sampling platform, be formed with the parallel accommodation hole that sets up side by side of multirow on the sample holder, and the quantity of every row of accommodation hole with fiber SPR detection mechanism's fibre channel quantity is the same, every place in the accommodation hole the sample cell.

In the technical scheme, the sampling needles are arranged in parallel, and the number of the sampling needles is the same as that of the accommodating holes in each row.

In the above technical scheme, the casing separates through a baffle and forms into kind chamber and detection chamber, the sampling platform of advance kind mechanism is installed at the appearance intracavity of advancing, waste liquid bottle and buffer bottle are placed in the inboard bottom of appearance chamber, optical fiber SPR detection mechanism installs detect the intracavity.

In the above technical scheme, a vertically arranged partition board is installed in the detection cavity, a plurality of bottom plates are installed on the partition board, and a connecting seat is correspondingly installed on each bottom plate to fix the optical fiber SPR sensor.

In the technical scheme, a reversing valve is arranged above the plunger pump and used for switching liquid in the capillary tube to enter the sample feeding mechanism or the liquid discharging mechanism.

In the technical scheme, ten valves in total are arranged on the valve station, wherein the valves are No. 1-10 valves; wherein, the No. 1-4 valve is communicated with the external atmosphere through a pipeline; one end of the No. 5 valve is communicated with the liquid drainage station, and the other end of the No. 5 valve is communicated with the waste liquid bottle; one end of the No. 6 valve, the No. 7 valve, the No. 9 valve and the No. 10 valve is communicated with the sampling needle, and the other end of the No. 6 valve, the No. 7 valve, the No. 9 valve and the No. 10 valve is communicated with the waste liquid cup; one end of the No. 8 valve is communicated with the liquid outlet of the waste liquid cup, and the other end is communicated with the liquid inlet of the waste liquid bottle.

In the above technical solution, the types of the optical fiber sensors connected in series in the internal fluid path may be the same or different.

In the technical scheme, a deep hole is formed in the connecting seat, the deep hole is horizontally formed, a sleeve is inserted in the deep hole, and the optical fiber is embedded in the sleeve.

In the technical scheme, the inner diameter of the sleeve is adjusted according to the outer diameter of the optical fiber so as to match the optical fibers with various different outer diameter specifications.

In the above technical solution, the volume of the internal fluid path of the optical fiber channel is 5 to 400 μ L.

In the above technical solution, the installation joint is an SMA905 joint.

The invention has the advantages and positive effects that:

1. optical fiber SPR detection system includes a plurality of parallelly connected fibre channel, and a plurality of fibre channel along angular distribution such as circumference on the coplanar, the sample flow path length that has guaranteed every fibre channel's inside liquid way equals and the dead volume is little, and set up a plurality of optical fiber sensor in every fibre channel, can sample same kind of sample and detect simultaneously or sample multiple sample and detect, thereby the detection of once sample can obtain multiunit optical fiber sensor's detected data, every inside liquid way is relatively independent and has the parallelism, the consumption of sample has effectively been reduced, reduce and detect the cost, and the detection efficiency is improved.

2. The sampling cleaning mechanism can clean the sampling needle in time after the sampling needle is subjected to sampling, and the problem that detection data is abnormal due to sample pollution in the next sampling process is avoided.

Drawings

FIG. 1 is a schematic diagram of a sample injection system according to the present invention;

FIG. 2 is a schematic diagram of the sample injection system of the present invention (only one capillary tube is shown with the remaining capillary tube omitted);

FIG. 3 is an enlarged view of a portion of the fiber channel of the present invention;

FIG. 4 is a schematic view of the structure of a sampling needle according to the present invention;

FIG. 5 is a schematic view of the construction of a sampling station according to the present invention;

FIG. 6 is a schematic view of the construction of the plunger pump of the present invention;

FIG. 7 is a schematic structural view of an optical fiber sensor according to the present invention;

FIG. 8 is a schematic diagram of a sample injection mechanism according to the present invention;

FIG. 9 is a schematic diagram of the drainage mechanism of the present invention;

FIG. 10 is a schematic view of the connection between the sample injection mechanism and the liquid discharge mechanism in the present invention.

In the figure:

1. sample introduction cavity 2, detection cavity 3 and first optical fiber channel

4. A second optical fiber channel 5, a third optical fiber channel 6 and a fourth optical fiber channel

7. Sampling platform 8, first sampling needle 9, second sampling needle

10. Third sampling needle 11, fourth sampling needle 12 and sample tube

13. Liquid discharge mechanism 14, waste liquid bottle 15 and buffer bottle

16. Plunger pump 17, peristaltic pump 18, valve station

19. Diaphragm pump 20, vacuum cup 21, capillary tube

22. Liquid discharge station 23, reversing valve 24 and capillary connector

25. Connecting seat 26, mounting connector 27 and optical fiber sensor

28. Optical fiber 29, sleeve 30, deep hole

31. Bottom plate 32, waste liquid cup

Detailed Description

The present invention will be described in further detail with reference to specific examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the scope of the invention in any way.

Example 1

As shown in the figure, the sample injection system suitable for the multi-channel terminal reflection type optical fiber SPR sensor comprises: the device comprises a shell, a sample introduction mechanism, an optical fiber SPR detection mechanism and a liquid discharge mechanism 13, wherein the sample introduction mechanism is arranged in the shell and used for collecting and conveying a sample to be detected, the optical fiber SPR detection mechanism is used for detecting the sample to be detected and is positioned at a terminal reflection type optical fiber SPR detection end, the liquid discharge mechanism 13 is used for self-cleaning and discharging detection waste liquid, the sample introduction mechanism is arranged at the front side sample introduction end of the optical fiber SPR detection mechanism, the liquid discharge mechanism 13 is arranged at the rear side sample discharge end of the optical fiber SPR detection mechanism, and the sample introduction mechanism, the optical fiber SPR detection mechanism and;

the sample introduction mechanism comprises a sample tube 12 for containing a sample to be detected, a sampling needle for collecting the sample to be detected in the sample tube 12, a peristaltic pump 17 for pumping the sample to be detected and pumping out detection waste liquid and a liquid discharge station 22 for receiving the detection waste liquid, which are sequentially communicated through a capillary tube 21, the sample tube 12 is arranged at the front sample inlet end of the optical fiber SPR detection mechanism, the sampling needle is positioned above the sample tube 12, the number of the sample tubes 12 is four, the number of the sampling needles is four, the sampling needles correspond to the sample tubes 12 respectively to collect samples to be detected, the liquid discharge station 22 is arranged at the rear sample outlet end of the optical fiber SPR detection mechanism, a sampling needle collects a sample to be detected and pumps the sample into the optical fiber SPR detection mechanism under the action of the peristaltic pump 17, after detection, detection waste liquid generated by the optical fiber SPR detection mechanism is conveyed to a liquid discharge station 22 through a capillary 21;

the optical fiber SPR detection mechanism consists of four optical fiber channels connected in parallel, a liquid inlet and a liquid outlet are formed at two ends of each optical fiber channel respectively, an internal liquid path is formed in each optical fiber channel, each internal liquid path is relatively independent and connected in parallel, a sample to be detected in each sampling needle is input into the optical fiber channel from the liquid inlet through a capillary tube 21 and is output from the liquid outlet through the internal liquid path, so that the internal liquid paths of the four optical fiber channels are connected in parallel and simultaneously acquire and detect the four samples, three optical fiber sensors 27 (adopting optical fiber SPR sensors) which are sequentially connected in series are installed in the internal liquid path of each optical fiber channel to form four-channel twelve-path detection, wherein a bottom plate 31 is installed in each optical fiber sensor 27, a connecting seat 25 is fixedly installed on the bottom plate 31, and a liquid inlet interface and a liquid outlet interface are formed at the top of the connecting, the capillary 21 is input through the liquid inlet interface and output through the liquid outlet interface, an optical fiber 28 is externally connected to the connecting seat 25, a sensor insertion opening is formed in one side of the connecting seat 25, each optical fiber sensor 27 is installed in the sensor insertion opening through an installation connector 26 (adopting an SMA905 connector), and the optical fiber is inserted into the optical fiber sensor 27 to be used for detecting a sample to be detected in the internal liquid path;

the four optical fiber channels comprise a first optical fiber channel 3, a second optical fiber channel 4, a third optical fiber channel 5 and a fourth optical fiber channel 6, the first optical fiber channel 3, the second optical fiber channel 4, the third optical fiber channel 5 and the fourth optical fiber channel 6 are distributed on the same plane along the circumference at equal angles and are connected in parallel through capillaries 21 and are used for simultaneously detecting the same sample for multiple times or simultaneously detecting multiple samples, each optical fiber channel is correspondingly communicated with one sampling needle through the capillary 21, under the action of the peristaltic pump 17, a sample to be detected collected by the sampling needle is pumped into an internal liquid path of the optical fiber channel and is detected by three optical fiber sensors 27 which are connected in series in sequence, and after detection, detection waste liquid is discharged into the liquid discharge mechanism 13 through a liquid outlet of the optical fiber channel;

the liquid discharge mechanism 13 comprises a buffer bottle 15 which is communicated through a capillary 21 and used for storing buffer liquid, a valve station 18 which is used for switching the flow direction of liquid in the capillary 21, a diaphragm pump 19 which is used for generating positive pressure or negative pressure, a vacuum cup 20 which is used for being matched with the diaphragm pump 19 to provide pressure and a waste liquid cup 32 which is used for receiving cleaning waste liquid, the buffer liquid in the buffer bottle is pumped into a sampling needle to be cleaned under the action of a plunger pump, the cleaning waste liquid after cleaning is discharged into the waste liquid cup under the action of the valve station, the diaphragm pump and the vacuum cup and is finally discharged into a waste liquid bottle to be collected.

Further, capillary connectors 24 are mounted on the liquid inlet and outlet of the connecting seat 25 for connecting with the capillary 21.

Further say, advance kind mechanism includes sampling platform 7 and sample holder, sample holder horizontal installation is on sampling platform 7, be formed with the parallel accommodation hole that sets up side by side of multirow on the sample holder, and the quantity of every row of accommodation hole is four, and the correspondence is placed in every accommodation hole sample tube 12, wherein: four the parallel arrangement of sampling needle, the sampling needle includes first sampling needle 8, second sampling needle 9, third sampling needle 10 and fourth sampling needle 11, first sampling needle 8 passes through

capillary

21 and 3 intercommunications of first fiber channel, second sampling needle 9 passes through

capillary

21 and 4 intercommunications of second fiber channel, third sampling needle 10 passes through

capillary

21 and 5 intercommunications of third fiber channel, fourth sampling needle 11 passes through

capillary

21 and 6 intercommunications of fourth fiber channel.

Further, the casing separates through a baffle and forms into kind chamber 1 and detection chamber 2, sampling platform 7 of sampling mechanism installs in kind chamber 1, waste liquid bottle 14 and buffer bottle 15 are placed in the inboard bottom of kind chamber 1, optic fibre SPR detection mechanism installs detect in the chamber 2.

Furthermore, a vertically arranged partition board is installed in the detection cavity 2, twelve bottom plates 31 are installed on the partition board, and a connecting seat 25 is correspondingly installed on each bottom plate 31 for fixing the optical fiber SPR sensor.

Further, a reversing valve 23 is installed above the plunger pump for switching the liquid in the capillary tube to enter the sample feeding mechanism or the liquid discharging mechanism 13.

The valve station comprises a No. 1-10 valve, wherein the No. 1-4 valve is communicated with the outside atmosphere through a pipeline, the No. 1 valve and the No. 3 valve are positive pressure air inlets, the No. 2 valve and the No. 4 valve are negative pressure air outlets, one end of the No. 5 valve is communicated with the liquid discharge station 22 through a pipeline, the other end of the No. 5 valve is communicated with the waste liquid bottle 14 through a pipeline to discharge the detection waste liquid in the liquid discharge station 22 into the waste liquid bottle 14, liquid inlet ends of the No. 6 valve, the No. 7 valve, the No. 9 valve and the No. 10 valve are respectively communicated with four sampling needles, the liquid outlet ends are respectively communicated with a liquid inlet of the waste cup 32 through a pipeline, the No. 8 valve is connected with a liquid outlet of the waste cup 32 to discharge the waste liquid bottle 14, a buffer solution in the buffer bottle 15 is pumped into the sampling needles through a plunger pump to be cleaned, and the cleaned cleaning waste liquid passes through the No. 6, The No. 9 valve and the No. 10 valve are conveyed into the waste liquid cup through the liquid inlet of the waste liquid cup 32, and then the cleaning waste liquid is discharged into the waste liquid bottle 14 through the No. 8 valve to be collected.

The vacuum cup comprises a vacuum cup 20, a valve 1, a valve 2, a valve 3 and a valve 4, wherein a pipeline communicated with the valve 1, the valve 2, the valve 3 and the valve 4 is an air channel, the valve 1 and the valve 3 are positive pressure air inlets, the valve 1 and the valve 3 are communicated through an air inlet pipeline, the valve 2 and the valve 4 are negative pressure air outlets, the valve 2 and the valve 4 are communicated through an air outlet pipeline, and an air outlet end of the air inlet pipeline and an air outlet end of the air outlet pipeline are communicated with the top end of the vacuum cup 20 through a tee joint to supply air or exhaust air to the vacuum cup so as to form positive pressure or negative pressure in the vacuum cup; the air is discharged back to the atmosphere environment from the waste liquid cup 32 through the vacuum cup 20, the valve No. 2, the peristaltic pump and the valve No. 4 to form negative pressure, and the formed negative pressure guides the cleaning waste liquid in the sampling needle into the waste liquid cup; the external air enters the vacuum cup 20 through the No. 1 valve, the No. 3 valve and the diaphragm pump 19 to form a positive pressure inside, and the formed positive pressure discharges the waste liquid in the waste liquid cup 32 to the waste liquid bottle 14.

Further, the principle of the liquid inlet mechanism is as follows (as shown in fig. 8):

the plunger pump 16 controls the sampling needle to extract a sample to be detected with a given volume in the sample tube 12, the sample to be detected is conveyed into the optical fiber channel through the capillary tube 21 under the action of the peristaltic pump 17, each sample to be detected enters the corresponding optical fiber channel and is detected by the three optical fiber sensors 27 connected in series in sequence, after the detection is finished, the detected sample is discharged into the liquid discharge station 22 and then enters the waste liquid bottle 14, and the waste liquid bottle 14 is taken out for emptying treatment after the bottle is visually filled.

Further, the principle of the liquid discharge mechanism 13 is as follows (as shown in fig. 9), wherein the liquid path and the gas path in fig. 9 are marked as follows: either the buffer liquid path or the sample liquid path,

is an exhaust pipeline, and is characterized by that it includes a gas-discharging pipeline,

is an air inlet pipeline.

When the sampling needle needs to be cleaned after the optical fiber SPR sensor is detected, the direction of the plunger pump is switched through the reversing valve 23, the plunger pump 16 extracts buffer liquid with a given volume in the buffer bottle 15, the diaphragm pump 19 is matched with the vacuum cup 20 to generate negative pressure so that the buffer liquid enters the sampling needle to be cleaned, the negative pressure is generated to guide the cleaning waste liquid into the waste liquid cup 32, the positive pressure is generated to discharge the cleaning waste liquid in the waste liquid cup into the waste liquid bottle 14, the sample is prevented from being polluted when the sampling needle is sampled next time, and the detection data are abnormal.

Further, the three optical fiber sensors 27 arranged in series in the internal fluid path may be the same type or different types.

Further, the volume of the internal liquid path of the optical fiber channel is 5-400 μ L.

The working principle of the sample injection system is as follows:

during sampling, the sample is quantitatively sampled through a sampling needle from a sample tube of the sampling platform under the action of a plunger pump, then a sample to be detected enters a corresponding optical fiber channel, the sample to be detected is detected in each optical fiber channel sequentially through three optical fiber sensors 27 connected in series, detection waste liquid generated after detection is discharged into a liquid discharge station, and the detection waste liquid in the liquid discharge station 22 is finally discharged into a waste liquid bottle for centralized collection.

After sampling is finished, the sampling needle needs to be cleaned, and the reversing valve 23 arranged above the plunger pump switches the direction and is switched to a cleaning mode for conveying buffer solution to the sampling needle. Under the action of the plunger pump, buffer solution in the buffer bottle is pumped into the sampling needles to clean the four sampling needles, the cleaned cleaning waste liquid enters the No. 6 valve through a pipeline, enters the No. 7 valve through a pipeline, enters the No. 9 valve through a pipeline, enters the No. 10 valve through the last pipeline, and the four cleaned cleaning waste liquids are discharged into the waste liquid cup 32 through pipelines.

The air is discharged from the waste liquid cup 32 to the external atmosphere through the vacuum cup 20, the valve No. 2, the peristaltic pump 19 and the valve No. 4 to form a negative pressure mode (namely an exhaust mode), and in the negative pressure mode, the cleaned cleaning waste liquid flows into the waste liquid cup 32 from the sampling platform 7 through the valves No. 6, 7, 9 and 10; then, adjusting air to enter the vacuum cup 20 from the peristaltic pump 19, the valve No. 1 and the valve No. 3 to form a positive pressure mode (namely an air inlet mode), and discharging the waste liquid in the waste liquid cup 32 to the waste liquid bottle 14 through pressure in the positive pressure mode; and the detection waste collected in the draining station 22 is drained into the waste bottle 14 through the No. 5 valve.

During sampling, the same sample can be sampled to be detected simultaneously or four samples can be sampled to be detected, the four optical fiber channels are distributed on the same plane along the circumference at equal angles, the lengths of sample flow paths of internal liquid paths of each optical fiber channel are equal, the dead volume is small, three optical fiber sensors 27 are arranged in each optical fiber channel, therefore, a detection path with twelve paths of four channels is formed, and detection data of twelve groups of optical fiber sensors 27 can be obtained through detection of one sample.

The arrangement of four channels and twelve channels can effectively reduce the consumption of the reflective terminal type optical fiber SPR sample, twelve groups of detection data are obtained by one-time detection, each internal liquid path is relatively independent and has parallelism, the lengths of the sample flow paths of the internal liquid paths are equal, the dead volume is reduced, the detection cost is effectively reduced, and the detection efficiency is improved.

In example 1, the layout of four channels and twelve channels is based on the maximum number of arrangements under the physical size conditions of the existing optical fiber SPR sensor, the base plate 31 and the partition plate. However, in the present invention, the arrangement is not limited to the arrangement of four channels and twelve channels, and the arrangement can be adjusted to the arrangement of multi-zone parallel connection and multi-channel by modifying the size.

Example 2

On the basis of embodiment 1, the sampling needle is installed on the casing through a driving mechanism, and the driving mechanism comprises a horizontal movement stepping motor and a vertical movement stepping motor for controlling the position movement of the sampling needle, so that the sampling needle moves to a required sample tube 12 to collect a sample to be measured.

Further, a deep hole 30 is formed in the connecting seat 25, and a sleeve 29 is inserted into the deep hole 30, and the optical fiber is embedded in the sleeve 29.

Further, the inner diameter of the ferrule 29 is adjusted to match the outer diameter of the optical fiber to match a plurality of different outer diameter specifications of optical fibers.

Spatially relative terms, such as "upper," "lower," "left," "right," and the like, may be used in the embodiments for ease of description to describe one element or feature's relationship to another element or feature as illustrated in the figures. It will be understood that the spatial terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "below" other elements or features would then be oriented "above" the other elements or features. Thus, the exemplary term "lower" can encompass both an upper and a lower orientation. The device may be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.

Moreover, relational terms such as "first" and "second," and the like, may be used solely to distinguish one element from another element having the same name, without necessarily requiring or implying any actual such relationship or order between such elements.

The invention has been described in an illustrative manner, and it is to be understood that any simple variations, modifications or other equivalent changes which can be made by one skilled in the art without departing from the spirit of the invention fall within the scope of the invention.

Claims

1. The utility model provides a sampling system suitable for multichannel terminal reflection type optic fibre SPR sensor which characterized in that: the device comprises a shell, a sample introduction mechanism, an optical fiber SPR detection mechanism and a liquid discharge mechanism, wherein the sample introduction mechanism is arranged in the shell and used for collecting and conveying a sample to be detected, the optical fiber SPR detection mechanism is used for detecting the sample to be detected and is positioned at a terminal reflection type optical fiber SPR detection end, the liquid discharge mechanism is used for self-cleaning and discharging detection waste liquid, the sample introduction mechanism is arranged at the front side sample introduction end of the optical fiber SPR detection mechanism, the liquid discharge mechanism is arranged at the rear side sample discharge end of the optical fiber SPR detection mechanism, and the sample introduction mechanism, the optical fiber SPR detection mechanism and;

2. The sample injection system of claim 1, wherein: capillary connectors are arranged on the liquid inlet interface and the liquid outlet interface of the connecting seat and are used for being connected with the capillary tubes.

3. The sample injection system of claim 2, wherein: the sampling needle passes through actuating mechanism and installs on the casing, actuating mechanism includes horizontal migration step motor and vertical migration step motor and removes in order to be used for controlling the position of sampling needle, makes the sampling needle remove to required sample cell play and gathers the sample that awaits measuring.

4. The sample injection system of claim 3, wherein: the sampling mechanism includes sampling platform and sample holder, sample holder horizontal installation is on the sampling platform, be formed with the parallel accommodation hole that sets up side by side of multirow on the sample holder, and the quantity of every row of accommodation hole with fiber SPR detection mechanism's fibre channel quantity is the same, every place in the accommodation hole the sample cell.

5. The sample injection system of claim 4, wherein: the sampling needles are arranged in parallel, and the number of the sampling needles is the same as that of the accommodating holes in each row.

6. The sample injection system of claim 5, wherein: the casing separates through a baffle and forms into kind chamber and detection chamber, the sampling platform of sampling mechanism installs at kind intracavity, waste liquid bottle and buffer bottle are placed in the inboard bottom of kind chamber, optic fibre SPR detection mechanism installs detect the intracavity.

7. The sample injection system of claim 6, wherein: the detection cavity is internally provided with a vertically arranged partition board, the partition board is provided with a plurality of bottom boards, and each bottom board is correspondingly provided with a connecting seat for fixing the optical fiber SPR sensor.

8. The sample injection system of claim 7, wherein: a deep hole is horizontally formed in the connecting seat, a sleeve is inserted in the deep hole, and the optical fiber is embedded in the sleeve.

9. The sample injection system of claim 8, wherein: the valve station is provided with ten valves in total, namely valves 1-10; wherein, the No. 1-4 valve is communicated with the external atmosphere through a pipeline; one end of the No. 5 valve is communicated with the liquid drainage station, and the other end of the No. 5 valve is communicated with the waste liquid bottle; one end of the No. 6 valve, the No. 7 valve, the No. 9 valve and the No. 10 valve is communicated with the sampling needle, and the other end of the No. 6 valve, the No. 7 valve, the No. 9 valve and the No. 10 valve is communicated with the waste liquid cup; one end of the No. 8 valve is communicated with the liquid outlet of the waste liquid cup, and the other end is communicated with the liquid inlet of the waste liquid bottle.

10. A method of using the sample injection system according to claim 9, comprising the steps of:

(1) during sampling, sampling is carried out quantitatively from a sample tube of a sampling platform through a sampling needle under the action of a plunger pump, then a sample to be detected enters a corresponding optical fiber channel, the sample to be detected is detected in each optical fiber channel sequentially through three optical fiber sensors connected in series, detection waste liquid generated after detection is discharged into a liquid discharge station, and the detection waste liquid in the liquid discharge station is finally discharged into a waste liquid bottle for centralized collection;

(2) after sampling is finished, the sampling needle needs to be cleaned, and a reversing valve arranged above the plunger pump switches the direction and is converted into a cleaning mode for conveying buffer solution to the sampling needle;

(3) during cleaning, under the action of a plunger pump, a buffer solution in a buffer bottle is pumped into the sampling needles to clean the four sampling needles, air is discharged from the waste liquid cup to the external atmosphere environment through the vacuum cup, the No. 2 valve, the peristaltic pump and the No. 4 valve to form a negative pressure mode (namely an exhaust mode), and the cleaned cleaning waste liquid flows into the waste liquid cup from the sampling table through the No. 6, 7, 9 and 10 valves under the negative pressure mode; then, adjusting air to enter the vacuum cup from the peristaltic pump, the valve No. 1 and the valve No. 3 to form a positive pressure mode (namely an air inlet mode), and discharging the waste liquid in the waste liquid cup to a waste liquid bottle through pressure in the positive pressure mode; and the detection waste liquid collected in the liquid discharge station is discharged into a waste liquid bottle through a No. 5 valve;

(4) and after the visual inspection of the waste liquid bottle is full, the waste liquid in the waste liquid bottle is led out.