CN113711049A

CN113711049A - Sample detection device and method for operating sample detection device

Info

Publication number: CN113711049A
Application number: CN201980095492.8A
Authority: CN
Inventors: 刘隐明; 滕锦; 石汇林; 向凤光
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2019-05-10
Filing date: 2019-05-10
Publication date: 2021-11-26
Also published as: WO2020227855A1

Abstract

A sample testing device (100) and a method of operating a sample testing device (100), the sample testing device (100) comprising a first reagent assembly (1), the first reagent assembly (1) comprising a first reagent container (11) for containing a first reagent and a first tubing (12); a second reagent assembly (2), the second reagent assembly (2) comprising a second reagent container (21) for containing a second reagent and a second conduit (22), the second reagent being distinct from the first reagent; the reaction tank (3) is used for processing the biological sample to form a sample to be reacted, and the reaction tank (3) is communicated with the first pipeline (12) and the second pipeline (22); and a partition (4), the partition (4) being arranged at least on the first conduit (12), the partition (4) being adapted to reduce contact of the first reagent with the reagent and/or the second reagent in the reaction cell (3). The sample detection device (100) can reduce the contact of the first reagent with the reagent and/or the second reagent in the reaction cell (3), reduce the risk of contamination of the first reagent with the reagent and/or the second reagent in the reaction cell (3), and reduce the consumption of the first reagent by arranging the partition (4) at least on the first pipeline (12) connected with the first reagent container (11).

Description

Sample detection device and method for operating sample detection device

Technical Field

The invention relates to the technical field of medical instruments, in particular to a sample detection device and an operation method of the sample detection device.

Background

The fluorescent reagent is used in middle and high-end hematology instrument products, the fluorescent dye is inserted into a DNA groove to change the structural freedom degree and the electronic state of dye molecules, so that the fluorescence characteristic of the molecules is changed to enhance the fluorescence, the cells are irradiated by laser, different cell structures emit different lights, signals are received by a receiver, and different cell classifications are obtained after processing.

Many current scenarios require consumption of fluorescent reagents, resulting in too rapid consumption of fluorescent reagents, where nearly half of the consumed fluorescent reagents are consumed in non-measured scenarios.

Disclosure of Invention

The present application provides a sample testing device and a method of operating a sample testing device.

In one aspect, an embodiment of the present application provides a sample detection device, including:

a first reagent assembly comprising a first reagent container for containing a first reagent and a first conduit;

a second reagent assembly comprising a second reagent container for holding a second reagent and a second conduit, the second reagent being distinct from the first reagent;

the reaction tank is used for processing a biological sample to form a sample to be detected, and the reaction tank is communicated with the first pipeline and the second pipeline; and

a separator disposed at least on the first conduit, the separator to reduce contact of the first reagent with a reagent within the reaction cell and/or the second reagent

On the other hand, an embodiment of the present application further provides an operation method of a sample detection device, which is applied to the sample detection device, where the sample detection device includes a first pipeline, a second pipeline, and a reaction cell, and the first pipeline and the second pipeline are communicated to the reaction cell, and the method includes:

cleaning and emptying the reaction tank;

causing the first conduit and/or the second conduit to draw back air to form a first column of air and causing the first column of air to be at least partially located in the first conduit;

and adding a base solution into the reaction tank.

According to the sample detection device provided by the embodiment of the application, the isolating piece is at least arranged on the first pipeline connected with the first reagent container, so that the contact between the first reagent and the reagent in the reaction tank and/or the second reagent can be reduced, the risk that the first reagent is polluted by the reagent in the reaction tank and/or the second reagent is reduced, and the consumption of the first reagent is reduced.

Drawings

FIG. 1 is a schematic view of a sample testing device provided in an embodiment of the present application;

FIG. 2 is a schematic flow chart of a sample testing method of the sample testing device shown in FIG. 1;

FIG. 3 is a schematic view of a reaction block in one embodiment of the sample testing device shown in FIG. 1;

FIG. 4 is a schematic view of a spacer in the reaction block shown in FIG. 3;

FIG. 5 is a schematic view of another angle of the spacer in the reaction block shown in FIG. 3;

FIG. 6 is a schematic view of a reaction assembly in another embodiment of the sample testing device shown in FIG. 1;

FIG. 7 is a schematic flow diagram of a sample detection method of the reaction assembly of FIG. 6;

FIG. 8 is a schematic flow diagram of another sample testing method of the sample testing device shown in FIG. 6;

FIG. 9 is a schematic view of a reaction block in another embodiment of the sample testing device shown in FIG. 6;

FIG. 10 is a schematic flow diagram of a sample testing method of the reaction assembly shown in FIG. 9;

FIG. 11 is a schematic view of a reaction block in another embodiment of the sample testing device shown in FIG. 6;

FIG. 12 is a schematic view of a reaction assembly in another embodiment of the sample testing device shown in FIG. 1;

FIG. 13 is a schematic flow diagram of a sample detection method of the reaction assembly of FIG. 12;

FIG. 14 is a schematic view of a reaction assembly in another embodiment of the sample testing device shown in FIG. 12;

FIG. 15 is a schematic view of a reaction assembly in another embodiment of the sample testing device shown in FIG. 12;

FIG. 16 is a schematic view of a reaction assembly in another embodiment of the sample testing device shown in FIG. 12.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, a sample testing device 100 is provided for testing a sample according to an embodiment of the present application, in which the sample testing device 100 is used for testing a sample. The reaction cell 3 in the sample testing device 100 is used for processing a sample to form a sample to be tested, and the sample to be tested can be transported to the testing component 5 for measurement. It should be noted that the specific type of the sample is not limited. In some embodiments, the sample comprises a fixed sample or a liquid sample. Further liquid samples include, but are not limited to, blood samples. It can be understood that the way in which the reaction cell 3 processes the sample to form the sample to be tested includes, but is not limited to, the following ways: the sample and various required reagents are subjected to incubation reaction to form a sample to be detected.

For the convenience of understanding the names of the stages of the samples, the names of the stages of the samples are detailed here; the sample is defined as a sample to be reacted before the incubation reaction of the sample in the reaction cell 3, and a reactant formed after the incubation reaction of the sample to be reacted and various reagents in the reaction cell 3 is defined as a sample to be detected.

It can be understood that, in order to prevent crystallization, the reaction cell 3 contains a diluent base solution, before a sample to be reacted reacts in the reaction cell 3, the diluent base solution in the reaction cell 3 needs to be emptied, so as to prevent the diluent base solution from diluting the sample to be reacted or a reagent reacting with the sample to be reacted, and in the prior art, the diluent base solution in the reaction cell 3 generally contacts with the reagent to be incubated with the sample to be reacted for a long time, so that the reagent is diluted, in other words, the reagent is contaminated by the diluent base solution, and the contaminated reagent needs to be drained to avoid abnormality in a test result of the sample to be tested, so that the reagent consumption is relatively large.

In order to solve the problem that reagents are easily polluted and the consumption amount is large, the embodiment of the application provides a sample detection device 100, the sample detection device 100 comprises a first reagent assembly 1, a second reagent assembly 2, a reaction pool 3 and a partition 4, the first reagent assembly 1 comprises a first reagent container 11 for containing a first reagent and a first pipeline 12; the second reagent assembly 2 comprises a second reagent container 21 for containing a second reagent, different from the first reagent, and a second conduit 22; the reaction tank 3 is used for processing a biological sample to form a sample to be detected, and the reaction tank 3 is communicated with the first pipeline 12 and the second pipeline 22; the spacer 4 is arranged at least on the first conduit 12, the spacer 4 being adapted to reduce contact of the first reagent with the reagent and/or the second reagent in the reaction cell 3.

Wherein, the first reagent in the first reagent component 1 and the second reagent in the second reagent component 2 can be incubated and reacted with the sample to be reacted in the reaction cell 3 to form the sample to be detected. Further, the first reagent includes, but is not limited to, a fluorescent liquid. The second reagent includes, but is not limited to, a hemolysis solution or other reagent solution. The reaction cell 3 is connected to the first pipeline 12 and the second pipeline 22, which means that the first reagent in the first pipeline 12 can flow into the reaction cell 3, and the second reagent in the second pipeline 22 can flow into the reaction cell 3. Wherein the spacer 4 is used for reducing the contact of the first reagent with the reagent in the reaction cell 3 and/or the second reagent may be understood as that the spacer 4 may be used for reducing the contact of the first reagent with the reagent in the reaction cell 3, the spacer 4 may be used for reducing the contact of the first reagent with the second reagent, and the spacer 4 may also be used for reducing the contact of the first reagent with the reagent in the reaction cell 3 and the second reagent.

By providing the spacer 4 at least on the first conduit 12, the contact of the first reagent, e.g. the fluorescent reagent, of the first conduit 12 assembly with the diluent of the reaction cell 3, or the contact of the first reagent, e.g. the fluorescent reagent, of the first conduit 12 assembly with the second reagent of the second conduit 22 assembly, or the contact of the fluorescent reagent with the diluent of the reaction cell 3 and the reagents of the second conduit 22 assembly is reduced, thereby reducing the consumption of the first reagent of the first conduit 12 assembly.

Referring to fig. 1 and fig. 3, in a sample testing device 100 according to an embodiment of the present disclosure, a first pipeline 12 and a second pipeline 22 in the sample testing device 100 are communicated with a reaction cell 3 through a partition 4. Specifically, the first tube 12 has a first access point S1, the second tube 22 has a second access point S2, the sample detection apparatus 100 further includes a third tube 31 connected to the reaction chamber 3, the third tube 31 has a third access point S3, and the first access point S1 and the second access point S2 are connected to the third access point S3 via a partition 4. In other words, the partition 4 is provided on both the first pipe 12 and the second pipe 22, and the first pipe 12 and the second pipe 22 communicate with the reaction tank 3 via the partition 4. In other words, the first reagent in the first pipeline 12 and the second reagent in the second pipeline 22 are in reduced contact with the diluted base liquid in the reaction tank 3 through the partition 4, so that the consumption of the first reagent in the first pipeline 12 assembly is reduced. The first reagent kit 1, the second reagent kit 2, the reaction cell 3, and the spacer 4 are mainly used for performing incubation reaction, and therefore, for convenience of description, the above components are collectively referred to as a reaction kit 10.

In some embodiments, as shown in fig. 1 and 3, the first reagent in the first reagent assembly 1 and the first reagent in the second reagent assembly 2 are completely isolated from the reagent in the third conduit 31 by the isolator 4.

In one embodiment, as shown in fig. 1, the spacer 4 comprises a first valve body and a first valve core located in the first valve body, the first valve body has two first inlets and one first outlet, the first access point S1 and the second access point S2 are respectively connected to one first inlet of the spacer 4, and the third access point S3 is connected to the first outlet of the spacer 4; the first valve spool moves relative to the first valve body to place the first access point S1 or the second access point S2 in communication with the third access point S3. Wherein the third access point S3 may be communicated with the first access point S1 or the third access point S3 may be communicated with the second access point S2 by controlling the energization of the first valve body and the first valve spool. Optionally, when the first valve body and the first valve core are in the power-off state, the third access point S3 is communicated with the second access point S2, in other words, when the first valve body and the first valve core are in the power-off state, the second reagent in the second pipeline 22 can flow into the reaction cell 3, and the first reagent in the first pipeline 12 cannot flow into the reaction cell 3 because of being isolated by the isolating piece 4, in other words, the reagent in the reaction cell 3 cannot diffuse into the first pipeline 12 to contaminate the first reagent in the first pipeline 12, so as to completely isolate the first reagent in the first pipeline 12 from the reaction cell 3; when the first valve body and the first valve core are in the energized state, the second reagent in the first pipeline 12 can flow into the reaction cell 3, and the second reagent in the second pipeline 22 cannot flow into the reaction cell 3 because of being isolated by the isolating piece 4.

As shown in fig. 2, fig. 2 is a flowchart illustrating an operation method of the sample detection device 100 shown in fig. 1, and the operation method of the sample detection device 100 includes: 101: and (4) emptying the diluent base solution in the reaction tank 3. Wherein the diluent base is emptied to prevent the diluent base from affecting the subsequent incubation reaction.

103: the second reagent in the second reagent container 21 flows into the reaction chamber 3 through the second channel formed by the second pipeline 22, the second access point S2 and the third access point S3, and the third pipeline 31 for the first time, and the second reagent is discharged.

This step is to prevent the diluent bottom liquid or the contaminants still remaining in the reaction chamber 3 from affecting the subsequent incubation reaction. The first valve core is in the power-off state, in other words, the second access point S2 and the third access point S3 are in the conducting state to form a second channel.

105: the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second channel formed by the second pipeline 22, the second access point S2 and the third access point S3, and the third pipeline 31.

Specifically, the second reagent is used for performing incubation reaction with the subsequent sample to be reacted and the first reagent to form the sample to be detected.

107: the partition 4 is in an electrified state, and the position of the first valve core relative to the first valve body is changed, so that the first access point S1 and the third access point S3 are communicated to form a first channel; and the first reagent in the first reagent container 11 flows into the reaction cell 3 through the first pipe 12, the first channel and the third pipe 31.

Specifically, the first reagent is used for performing an incubation reaction with the second reagent contained in the reaction cell 3 and a subsequent sample to be reacted to form a sample to be detected.

109: the partition 4 is in the power-off state, the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipeline 22, the second channel and the third pipeline 31 for the third time, and the sample to be reacted is added into the reaction cell 3.

Specifically, the sample to be reacted, the first reagent and the second reagent are subjected to incubation reaction to form the sample to be detected.

111: the sample to be measured flows into the detection assembly 5 for measurement;

113: and cleaning liquid is added into the reaction tank 3 for cleaning, and base liquid is added after the reaction tank 3 is cleaned.

It is understood that the second reagent may be added only once in the operation method of the sample detection apparatus 100 under the test condition that the accuracy is not high, in other words,

steps

103 and 105 may be omitted. It is understood that the sequence of the first reagent, the second reagent and the sample to be reacted flowing into the reaction chamber in the operation method of the sample detection apparatus 100 shown in fig. 2 is an alternative embodiment, and the sequence of the first reagent, the second reagent and the sample to be reacted can be changed. For example, the first reagent, the second reagent, and the sample to be reacted may be added simultaneously, sequentially, or the like.

It is understood that the sample to be reacted is a blood sample. Further, the base solution may be a diluent.

The sample detection device 100 provided by the embodiment of the present application sets the partition member 4 to a structure including the first valve body and the first valve core, so that the first pipeline 12 and the reaction cell 3 are conducted only when the first reagent is added to the reaction cell 3, and the diluent in the reaction cell 3 and the second reagent in the second pipeline 22 are completely isolated by the partition member 4 and cannot diffuse into the first pipeline 12, so that the first reagent in the first reagent container 11 cannot be polluted by the diluent in the reaction cell 3 and the second reagent in the second pipeline 22, and thus the consumption of the first reagent is reduced.

In another embodiment, as shown in fig. 3, fig. 3 is a schematic view of the reaction assembly 10 of the sample testing assembly 5 provided in fig. 1, and the partition 4 can also be configured to completely separate the first reagent in the first reagent assembly 1 and the second reagent in the second reagent assembly 2 from the reagent in the third pipeline 31 through the partition 4. Specifically, referring to fig. 4 and 5 together, fig. 4 and 5 are schematic views of the spacer 4 in the reaction module 10 shown in fig. 3. The partition 4 includes a second valve body 43 and a second valve spool 44 located in the second valve body 43, the second valve body 43 has two second inlets 43a and one second outlet 43b, the first access point S1 and the second access point S2 are respectively accessed to the one second inlet 43a of the partition 4, and the third access point S3 is accessed to the second outlet 43b of the partition 4; when the second spool 44 is in the first position of the second valve body 43, the first access point S1 is disconnected from the third access point S3, and the second access point S2 is communicated with the third access point S3; when the second spool 44 is in the second position of the second valve body 43, the first access point S1 is in communication with the third access point S3, and the second access point S2 is in communication with the third access point S3.

Alternatively, the two second inlets 43a and the one second outlet 43b are located on the same side of the second valve body 43, so as to facilitate the pipeline access to the second valve body 43. Further, the second inlet 43a for connecting to the first pipe 12 is located between the second inlet 43a for connecting to the second pipe 22 and the second outlet 43b for connecting to the third pipe 31. When the partition 4 is in the power-off state, the second valve body 43 blocks the second inlet 43a for accessing the first pipeline 12, and correspondingly, the second inlet 43a for accessing the second pipeline 22 is communicated with the second port, so that the second reagent in the second pipeline 22 can flow into the reaction tank 3; when partition 4 is in the energized state, second valve body 43 moves in a direction away from second inlet 43a for accessing first conduit 12 to communicate second inlet 43a with second outlet 43b for accessing first conduit 12, thereby enabling the first reagent in first conduit 12 to flow into reaction cell 3.

Please refer to step 101-113 for a process of the operation method of the sample detection apparatus 100, which is not described herein.

In some embodiments, as shown in fig. 6, 9 and 11, the separator 4 may also reduce consumption of the first reagent in the first conduit 12 by reducing contact of the first reagent with other reagents by not completely separating the first reagent from the other reagents. Specifically, as shown in fig. 6, the partition 4 has a connector 45, the connector 45 has an inner cavity and three ports communicating with the inner cavity, and the first access point S1, the second access point S2 and the third access point S3 are respectively connected to one port of the connector 45. Optionally, the inner diameter of the interface of the connector 45 entering the first access point S1 is 0.1mm to 0.35 mm. Specifically, the joint 45 may be a three-way joint 45, and the inner diameter of the joint 45 connected to the first access point S1 is changed, so that the second reagent in the second pipeline 22 or the reagent in the reaction tank 3 is difficult to diffuse into the first pipeline 12, thereby reducing the contact between the first reagent in the first pipeline 12 and other reagents, and reducing the consumption of the first reagent in the first pipeline 12.

As shown in fig. 7, fig. 7 is a flowchart illustrating an operation method of the sample detection device 100 shown in fig. 6, and the operation method of the sample detection device 100 includes: :

201: and (4) emptying the diluent base solution in the reaction tank 3. Wherein the diluent base is emptied to prevent the diluent base from affecting the subsequent incubation reaction.

203: the second reagent in the second reagent container 21 flows into the reaction tank 3 through the second pipeline 22, the joint 45 and the third pipeline 31 for the first time in sequence and is discharged, so that the diluent base solution still remaining in the reaction tank 3 is prevented from affecting the subsequent incubation reaction.

205: the second reagent in the second reagent container 21 is introduced into the reaction cell 3 through the second pipe 22, the joint 45, and the third pipe 31 in this order for the second time. The second reagent is used for carrying out incubation reaction with the subsequent sample to be reacted and the first reagent to form a sample to be detected.

207: the first reagent in the first reagent container 11 is introduced into the reaction cell 3 through the first pipe 12, the joint 45, and the third pipe 31. The first reagent is used for carrying out incubation reaction with the second reagent contained in the reaction tank 3 and a subsequent sample to be reacted to form a sample to be detected.

209: the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipeline 22, the joint 45 and the third pipeline 31 for the third time, and the sample to be reacted is added into the reaction cell 3 at the same time. The sample to be reacted, the first reagent and the second reagent are subjected to incubation reaction to form a sample to be detected.

211: the sample to be measured flows into the detection assembly 5 for measurement;

213: and cleaning liquid is added into the reaction tank 3 for cleaning, and base liquid is added after the reaction tank 3 is cleaned.

It is understood that the second reagent may be added only once during the operation of the sample detection apparatus 100 under the test condition that the accuracy is not high, in other words,

steps

203 and 205 may be omitted. It is understood that the order of adding the first reagent, the second reagent and the sample to be reacted in the operation method of the sample detection apparatus 100 shown in fig. 7 is an alternative embodiment, and the order of adding the first reagent, the second reagent and the sample to be reacted may be changed. For example, the first reagent, the second reagent, and the sample to be reacted may be added simultaneously, or sequentially, etc.

In the sample detection device 100 provided by the embodiment of the present application, the partition 4 is configured as the joint 45, the joint 45 is respectively communicated with the first pipeline 12, the second pipeline 22 and the third pipeline 31, and the inner diameter of the joint 45 connected with the first pipeline 12 is smaller, so that the diffusion of the reagent in the second pipeline 22 or the third pipeline 31 into the first pipeline 12 can be preferably reduced, and the consumption of the first reagent in the first pipeline 12 can be reduced.

In one embodiment, referring to fig. 6, the isolation member 4 further includes a first air column located in the first pipeline 12 and near the first reagent container 11. The first air column is formed by air, and when the first air column is positioned in the first pipeline 12, other reagents can be effectively prevented from being diffused into the first pipeline 12, so that the reagents in the first pipeline 12 are prevented from being polluted, and the consumption of the reagents in the first pipeline 12 is reduced.

Referring to fig. 8, fig. 8 is a flowchart illustrating an operation method of the sample testing device 100 shown in fig. 6, the operation method of the sample testing device 100 is performed after the step of cleaning and emptying the reaction cell 3 after the reaction cell 3 forms a sample to be tested, in other words, the method includes:

301: cleaning and emptying the reaction tank;

303: causing the first conduit to draw back air to form a first column of air and causing the first column of air to be at least partially located in the first conduit;

305: adding a base solution into the reaction tank.

Optionally, when the sample detection apparatus 100 needs to form a next sample to be tested in the reaction cell 3, i.e. perform a sample test, please refer to steps 201 to 213, which are not described herein again. Wherein the first gas column is eliminated during the inflow. In other words, the first tube 12, which is the tube in which the first gas column is formed, is broken when the first reagent passes through the first tube. Of course, in other embodiments, the first column of gas may also enter the second conduit 22, in other words, the first column of gas may also be located in the second conduit 22. If the first air column is located in the second pipeline 22, step 301 may correspond to the first air column being formed by the first pipeline 12 and the second pipeline 22 sucking back air, and the first air column may be broken when the first air column is first passed through the second pipeline 22. Of course, in other embodiments, the first gas column may also be located only in the second conduit 22.

The sample detection device 100 provided by the embodiment of the present application sets the partition 4 as the joint 45 and the first gas column located in the first pipeline 12 and/or the second pipeline 22, and when the first gas column is located in the pipeline, other reagents can be effectively prevented from diffusing into the pipeline, so that the reagents in the pipeline are prevented from being polluted, and the consumption of the reagents in the pipeline is reduced.

In another embodiment, as shown in fig. 9, the isolating member 4 further has a two-way valve 46, and the two-way valve 46 is disposed on the first pipeline 12 and located between the first reagent container 11 and the first access point S1. Alternatively, the internal volume of the two-way valve 46 is small, and the two-way valve 46 is close to the joint 45. The two-way valve 46 can effectively prevent other reagents from flowing into the first reagent vessel 11 through the joint 45, thereby reducing the consumption of the first reagent.

Referring to fig. 10, fig. 10 is a flowchart illustrating an operation method of the sample detection device 100 shown in fig. 9, the operation method of the sample detection device 100 includes: 401: and (4) emptying the diluent base solution in the reaction tank 3. Wherein the diluent base is emptied to prevent the diluent base from affecting the subsequent incubation reaction.

403: the second reagent in the second reagent container 21 flows into the reaction tank 3 through the second pipeline 22, the joint 45 and the third pipeline 31 for the first time in sequence and is discharged, so that the diluent base solution still remaining in the reaction tank 3 is prevented from affecting the subsequent incubation reaction.

405: the second reagent in the second reagent container 21 is introduced into the reaction cell 3 through the second pipe 22, the joint 45, and the third pipe 31 in this order for the second time. The second reagent is used for carrying out incubation reaction with the subsequent sample to be reacted and the first reagent to form a sample to be detected.

407: the two-way valve 46 is opened, the first reagent in the first reagent container 11 flows into the reaction tank 3 through the first pipeline 12, the two-way valve 46, the joint 45 and the third pipeline 31, and then the two-way valve 46 is closed. The first reagent is used for carrying out incubation reaction with the second reagent contained in the reaction tank 3 and a subsequent sample to be reacted to form a sample to be detected.

409: the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipeline 22, the joint 45 and the third pipeline 31 for the third time, and the sample to be reacted is added into the reaction cell 3 at the same time. The sample to be reacted, the first reagent and the second reagent are subjected to incubation reaction to form a sample to be detected.

411: the sample to be measured flows into the detection assembly 5 for measurement;

413: and cleaning liquid is added into the reaction tank 3 for cleaning, and base liquid is added after the reaction tank 3 is cleaned.

In yet another embodiment, as shown in fig. 11, the partition 4 further has a check valve 47, and the check valve 47 is disposed on the first pipeline 12 and located between the first reagent container 11 and the first access point S1.

Optionally, the interface of the connector 45 connected to the first access point S1 is connected to the outlet of the one-way valve 47. In other words, the joint 45 and the check valve 47 are integrated. In other words, no hose connection is required between the connector 45 and the one-way valve 47. The partition 4 of this structure enables the first reagent in the first pipeline 12 to flow into the reaction tank 3 from the partition 4, and the corresponding second reagent in the second pipeline 22 and the reagent in the reaction tank 3 cannot flow through the first reagent container 11 through the check valve 47 due to the existence of the check valve 47, so that the first reagent is prevented from being polluted by other reagents, and the consumption of the first reagent is reduced.

The reaction process of the sample detection device 100 is substantially the same as that of steps 401 to 413, except that: the first reagent in the first reagent container 11 is introduced into the reaction cell 3 through the first line 12, the check valve 47, the joint 45, and the third line 31. The first reagent is used for carrying out incubation reaction with the second reagent contained in the reaction tank 3 and a subsequent sample to be reacted to form a sample to be detected.

As shown in fig. 12 to 17, in the reaction module 10 provided in the embodiment of the present application, the first pipeline 12 and the second pipeline 22 are respectively connected to the reaction cell 3, so that the mutual contamination between the first reagent in the first pipeline 12 and the second reagent in the second pipeline 22 is completely isolated, and the partition 4 is only disposed on the first pipeline 12 to reduce the contact between the first reagent in the first pipeline 12 and the reagent in the reaction cell 3, thereby reducing the consumption of the first reagent in the first pipeline 12. Specifically, the first pipeline 12 and the second pipeline 22 are respectively connected with the reaction tank 3, and the partition 4 is arranged on the first pipeline 12.

In one embodiment, as shown in fig. 12, a partition 4 is formed at a section of the first pipe 12 connected to the reaction tank 3, and the inner diameter of the partition 4 is the minimum inner diameter of the first pipe 12, and ranges from 0.1mm to 0.35 mm. Specifically, the isolation member 4 may be a portion of the first pipeline 12, and the isolation member 4 may also be a steel pipe sleeved on an end portion of the first pipeline 12, and an inner diameter of the steel pipe is 0.1mm to 0.35 mm. By making the inner diameter of the section of the first pipeline 12 connecting the reaction tank 3 be the minimum inner diameter of the first pipeline 12, the diffusion speed of the reagent in the reaction tank 3 to the first pipeline 12 is made to reduce the amount of the first reagent in the first pipeline 12 polluted by the reagent in the reaction tank 3, thereby reducing the consumption of the first reagent in the first pipeline 12.

Referring to fig. 13, fig. 13 is a flowchart illustrating an operation method of the sample detection device 100 shown in fig. 12, the operation method of the sample detection device 100 includes:

501: and (4) emptying the diluent base solution in the reaction tank 3. Wherein the diluent base is emptied to prevent the diluent base from affecting the subsequent incubation reaction.

503: the second reagent in the second reagent container 21 flows into the reaction tank 3 through the second pipeline 22 for the first time and is drained, so as to prevent the diluent bottom liquid still remaining in the reaction tank 3 from affecting the subsequent incubation reaction.

505: the second reagent in the second reagent container 21 is introduced into the reaction cell 3 through the second pipe 22 for the second time. The second reagent is used for carrying out incubation reaction with the subsequent sample to be reacted and the first reagent to form a sample to be detected.

507: the first reagent in the first reagent container 11 is introduced into the reaction cell 3 through the first pipe 12. The first reagent is used for carrying out incubation reaction with the second reagent contained in the reaction tank 3 and a subsequent sample to be reacted to form a sample to be detected.

509: the second reagent in the second reagent container 21 flows into the reaction cell 3 through the second pipeline 22 for the third time, and the sample to be reacted is added into the reaction cell 3 at the same time. The sample to be reacted, the first reagent and the second reagent are subjected to incubation reaction to form a sample to be detected.

511: the sample to be measured flows into the detection assembly 5 for measurement;

513: and cleaning liquid is added into the reaction tank 3 for cleaning, and base liquid is added after the reaction tank 3 is cleaned.

In another embodiment, as shown in fig. 12, the isolation member 4 is a second gas column located in the first pipeline 12 for blocking the communication between the first reagent and the reaction chamber 3. In other words, the first pipe 12 and the second pipe 12 are respectively connected to the reaction chamber 3, and the first pipe 12 sucks air back to form a second air column. Specifically, the second air column is formed by air, and when the second air column is located in the first pipeline 12, other reagents can be effectively prevented from being diffused into the first pipeline 12, so that the reagents in the first pipeline 12 are prevented from being polluted, and the consumption of the reagents in the first pipeline 12 is reduced. It will be appreciated that the second gas column herein may be the same as the first gas column described previously.

After the operation of the operation method of the sample detection apparatus 100 is performed in the step of cleaning and emptying the reaction cell 3 after the reaction cell 3 forms a sample to be detected once, please refer to steps 301 to 305 for details, which are not described herein again.

Optionally, when the sample detection apparatus 100 needs to form a next sample to be detected in the reaction cell 3, please refer to steps 501 to 513, and details are not repeated herein. Wherein the first tube 12, which is a tube in which the first gas column is formed, is broken when the first reagent passes through the first tube.

In yet another embodiment, as shown in fig. 14 and 15, the isolator 4 is a two-way or one-way valve in series with the first conduit 12.

Alternatively, as shown in fig. 14, the partition 4 may be a two-way valve connected in series in the first pipe 12, the two-way valve having a small inner volume and being located near the joint 45. The two-way valve can effectively prevent the reagent in the reaction cell 3 from flowing into the first reagent container 11, thereby reducing the consumption of the first reagent.

Alternatively, as shown in fig. 15, the partition 4 may be a check valve connected in series in the first pipeline 12, the partition 4 of this structure enables the first reagent in the first pipeline 12 to flow into the reaction tank 3 from the partition 4, and correspondingly, due to the existence of the check valve, the reagent in the reaction tank 3 cannot flow through the first reagent container 11 through the check valve, so as to avoid the first reagent being polluted by other reagents, thereby reducing the consumption of the first reagent.

The reaction flow of the sample detection device 100 is substantially the same as that of steps 501 to 513, except that the first reagent in the first reagent container 11 is flowed into the reaction cell 3 through the first pipeline 12, the two-way valve or the one-way valve.

In still another embodiment, as shown in fig. 16, the second pipeline 22 is connected to the reaction chamber 3, the first pipeline 12 is connected to the reaction chamber 3 through a spacer 4, and the spacer 4 is a sleeve pipe and is suspended above the reaction chamber 3. Specifically, the first pipeline 12 is suspended above the reaction tank 3, so that the mutual diffusion speed between the first pipeline 12 and the reaction tank 3 is reduced, and the consumption of the first reagent in the first pipeline 12 is reduced.

Please refer to steps 501 to 513, and the detailed description thereof is omitted.

Further, as shown in fig. 1, the sample testing device 100 further includes a main pipeline 6 and a testing component 5, the main pipeline 6 has a fourth access point S4, and the reaction cell 3 is connected to the fourth access point S4 of the main pipeline 6; the detecting assembly 5 includes a sample needle 51, the sample needle 51 has a fifth access point S5, and the sample to be tested in the reaction cell 3 flows to the fifth access point S5 of the sample needle 51 through the fourth access point S4 of the main pipeline 6, so that the sample to be tested in the reaction cell 3 flows into the sample in the detecting assembly 5 for detection.

Optionally, the sample testing device 100 further includes a fourth pipeline 32 connected between the reaction cell 3 and the main pipeline 6, and a first switch 33 disposed on the fourth pipeline 32, wherein the first switch 33 is located between the fourth access point S4 and the main pipeline 6. If the first switching member 33 is opened, that is, the first switching member 33 realizes communication between the reaction cell 3 and the main pipeline 6, the sample to be tested after the reaction in the reaction cell 3 can flow to the fifth access point S5 of the sample needle 51 through the fourth pipeline 32 and the fourth access point S4 in sequence, so as to enter the detection assembly 5 for detection. When the first switch 33 is closed, that is, when the first switch 33 disconnects the inner fourth line 32, the liquid to be measured in the fourth line 32 cannot flow into the main line 6.

Optionally, the sample detection apparatus 100 further includes a fifth pipeline 61 connected to the fourth access point S4 and a first diluent pool 62 connected to the fifth pipeline 61. The diluent in the first diluent pool 62 can flow into the reaction pool 3 through the fifth pipe 61, the fourth access point S4 and the fourth pipe 32.

Optionally, the sample detection apparatus 100 further comprises a drain 34 connected to the reaction well 3. The drain tank 34 can be used to store the liquid discharged from the reaction tank 3.

Optionally, the detection assembly 5 further comprises a flow chamber 52, a sheath fluid reservoir 53 and a first waste fluid reservoir 54. The flow chamber 52 has a sample inlet to be measured connected to the fifth access point S5 of the sample needle 51, a sheath liquid inlet connected to the sheath liquid pool 53, the sheath liquid pool 53 for storing sheath liquid, and an outlet for connecting to the first waste liquid pool 54.

Optionally, the sheath liquid pool 53 is connected to a first air storage tank, and when the two are communicated, the first positive pressure provided by the first air storage tank pushes the sheath liquid to flow into the flow chamber 52.

When the detection assembly 5 performs detection, a sample to be detected enters the flow chamber 52 from a sample inlet to be detected, sheath liquid in the sheath liquid pool 53 can flow into the flow chamber 52 through the sheath liquid inlet, the sample to be detected is wrapped by the sheath liquid to form a sample flow to be detected for detection, and the detected sample flow to be detected enters the first waste liquid pool 54 from an outlet.

Further, the sample testing device 100 further comprises a sample pushing assembly 7, and the sample to be tested of the sample preparation section to be tested is pushed into the sample needle 51 of the testing assembly 5 by arranging the sample pushing assembly 7.

The sample pushing assembly 7 is respectively connected to a sixth access point S6 of the main pipeline 6, the sixth access point S6 is located between the fourth access point S4 and the fifth access point S5, and a channel between the sixth access point S6 and the fifth access point S5 is a sample preparation section to be tested; the sample to be tested in the reaction cell 3 flows to the sixth access point S6 of the main pipeline 6 through the fourth access point S4 of the main pipeline 6, and flows into the sample preparation section to be tested through the sixth access point S6 of the main pipeline 6; the sample pushing assembly 7 is used for pushing the sample to be tested in the sample preparation section to be tested into the sample needle 51 of the detecting assembly 5.

Optionally, the sample pushing assembly 7 includes a syringe 71, a sixth pipeline 72, a second switching member 73 disposed on the sixth pipeline 72, and a second diluent pool 74. When the second switch 73 is opened, the diluent in the second diluent pool 74 can flow into the syringe 71; the liquid in the syringe 71 is pushed from the sixth access point S6 into the fifth access point S5, so that the sample to be tested in the sample segment to be tested is pushed into the fifth access point S5 for being tested by the testing assembly 5. The second switching member 73 is turned off to cut off the sixth pipe 72.

Optionally, the sample detection apparatus 100 further includes a third switch 63 disposed on the main pipeline 6, and the third switch 63 is located between the sixth access point S6 and the fourth access point S4. When the third switch 63 is turned on, the fourth access point S4 is connected to the sample preparation segment to be tested. When the third switch 63 is closed, the main line 6 is shut off.

Further, the sample testing device 100 further comprises a sampling assembly 8, the sampling assembly 8 is connected to the end of the main pipeline 6, and the sampling assembly 8 is used for forming negative pressure to extract the sample to be tested in the reaction tank 3 into the sample preparation section to be tested.

Optionally, the sampling assembly 8 includes a second waste liquid pool 81, and a negative pressure is formed in the second waste liquid pool 81, and the negative pressure extracts the sample to be detected in the reaction pool 3 into the sample preparation section to be detected.

When the sample detection device 100 needs to perform detection, an incubation reaction is performed in the reaction cell 3 (please refer to the reaction process mentioned in the previous embodiment for the specific reaction process of the reaction component 10, which is not described herein again), and a sample to be detected after the incubation reaction flows into the detection component 5 for detection. Wherein, the flow path of the sample to be measured is: the sampling assembly 8 is made to form negative pressure, the first switching piece 33 and the third switching piece 63 are opened, so that the sample to be detected flows through the fourth pipeline 32, the fourth access point S4 and the main pipeline 4 from the reaction tank 3 in sequence until the sample to be detected flows into the sample preparation section, the second switching piece 73 is opened, and the diluent in the second diluent tank 74 flows into the injector 71; the liquid in the syringe 71 is pushed into the fifth access point S5 from the sixth access point S6, so that the sample to be tested in the sample segment to be tested is pushed into the fifth access point S5 for the testing module 5 to test; when the detection assembly 5 performs detection, a sample to be detected enters the flow chamber 52 from a sample inlet to be detected, sheath liquid in the sheath liquid pool 53 can flow into the flow chamber 52 through the sheath liquid inlet, the sample to be detected is wrapped by the sheath liquid to form a sample flow to be detected for detection, and the detected sample flow to be detected enters the first waste liquid pool 54 from an outlet.

The sample testing device 100 provided by the embodiment of the present application reduces the contact between the first reagent, such as the fluorescent reagent, of the first pipeline 12 assembly and the diluent of the reaction chamber 3, or reduces the contact between the first reagent, such as the fluorescent reagent, of the first pipeline 12 assembly and the second reagent of the second pipeline 22 assembly, or reduces the contact between the fluorescent reagent and the diluent of the reaction chamber 3 and the reagent of the second pipeline 22 assembly, by disposing the spacer 4 at least on the first pipeline 12, thereby reducing the consumption of the first reagent of the first pipeline 12 assembly.

Claims

A sample testing device, comprising:

a first reagent assembly comprising a first reagent container for containing a first reagent and a first conduit;

a second reagent assembly comprising a second reagent container for holding a second reagent and a second conduit, the second reagent being distinct from the first reagent;

the reaction tank is used for processing a biological sample to form a sample to be detected, and the reaction tank is communicated with the first pipeline and the second pipeline; and

a separator disposed at least on the first conduit, the separator to reduce contact of the first reagent with a reagent within the reaction cell and/or the second reagent.
The sample testing device of claim 1, wherein said first conduit has a first access point, said second conduit has a second access point, said sample testing device further comprising a third conduit connecting said reaction cell, said third conduit has a third access point, and said first access point and said second access point communicate with said third access point via said partition.
The sample testing device of claim 2, wherein the first reagent in the first reagent assembly and the second reagent in the second reagent assembly are completely isolated from the reagent in the third conduit by the isolator.
The sample testing device of claim 3, wherein the spacer comprises a first valve body and a first valve spool located within the first valve body, the first valve body having two first access ports and a first access port, the first access point and the second access point each accessing one of the first access ports of the spacer, and the third access point accessing the first access port of the spacer; the first valve spool moves relative to the first valve body to communicate the first access point or the second access point with the third access point.
The sample testing device of claim 3, wherein the barrier member comprises a second valve body and a second valve spool located within the second valve body, the second valve body having two second access ports and a second access port, the first access point and the second access point each accessing one of the second access ports of the barrier member, and the third access point accessing the second access port of the barrier member; when the second valve body is located at the first position of the second valve body, the first access point is disconnected from the third access point, and the second access point is communicated with the third access point; when the second valve spool is in the second position of the second valve body, the first access point is in communication with the third access point, and the second access point is in communication with the third access point.
The sample testing device of claim 2, wherein said spacer has a connector having a lumen and three ports communicating with said lumen, said first access point, said second access point and said third access point each accessing one port of said connector.
The sample testing device of claim 6, wherein the interface of said adapter into said first access point has an inner diameter of 0.1mm to 0.35 mm.
The sample testing device of claim 6 or 7, wherein the separator further comprises a first gas column located at least partially in the first and/or second conduit and proximate to the second reagent container.
The sample testing device of claim 1 or 7, wherein the barrier further comprises a two-way valve disposed on the first conduit between the first reagent container and the first access point.
The sample testing device of claim 1 or 7, wherein the spacer further comprises a one-way valve disposed on the first conduit between the first reagent container and the first access point.
The sample testing device of claim 10, wherein the interface of said adapter into said first access point is into the outlet of said one-way valve.
The apparatus according to claim 1, wherein the first and second pipes are connected to the reaction cells, respectively, and the separator is disposed on the first pipe.
The apparatus according to claim 12, wherein the partition is formed at a section of the first pipe connecting the reaction cell, and has an inner diameter of a minimum inner diameter of the first pipe, and the inner diameter ranges from 0.1mm to 0.35 mm.
The sample testing device of claim 12, wherein said barrier is a two-way or one-way valve in series with said first conduit.
The sample testing device of claim 12, wherein the separator is a second gas column disposed in the first conduit for blocking communication between the first reagent and the reaction cell.
The apparatus according to claim 1, wherein the second tube is connected to the reaction chamber, the first tube is connected to the reaction chamber via the partition, and the partition is a sleeve and is suspended above the reaction chamber.
The sample testing device of any of claims 1 to 16, wherein the first reagent assembly is configured to receive a fluorescent reagent.
The sample testing device of claim 17, wherein the second reagent assembly is adapted to contain at least one of a hemolytic agent and a diluent.
The sample testing device of any one of claims 1 to 18, further comprising:

the main pipeline is provided with a fourth access point, and the reaction pool is connected to the fourth access point of the main pipeline;

a detection assembly including a sample needle having a fifth access point, the sample to be detected of the first reaction cell flowing through the fourth access point of the main pipeline to the fifth access point of the sample needle, so that the sample to be detected in the first reaction cell flows into the sample in the detection assembly for detection.
The apparatus according to claim 19, further comprising sample pushing components respectively connected to sixth access points of the main pipeline, the sixth access points being located between the fourth access point and the fifth access point, and a channel between the sixth access point and the fifth access point being a section for preparing samples to be tested;

the sample to be detected in the reaction pool flows to a sixth access point of the main pipeline through the fourth access point of the main pipeline and flows into the sample preparation section to be detected through the sixth access point of the main pipeline;

the sample pushing assembly is used for pushing the sample to be detected in the sample preparation section to be detected into the sample needle of the detection assembly.
The sample testing device of claim 20, further comprising a sampling assembly coupled to an end of said main conduit, said sampling assembly configured to create a negative pressure to draw a sample to be tested from said reaction cell into said sample preparation section.
An operation method of a sample detection device is applied to the sample detection device, the sample detection device comprises a first pipeline, a second pipeline and a reaction pool, the first pipeline and the second pipeline are communicated with the reaction pool, and the method is characterized by comprising the following steps:

cleaning and emptying the reaction tank;

causing the first conduit and/or the second conduit to draw back air to form a first column of air and causing the first column of air to be at least partially located in the first conduit;

and adding a base solution into the reaction tank.
The method of claim 22, wherein the first and second conduits are each in communication with the reaction cell, and wherein "causing the first and/or second conduits to suck back air to form a first column of air" comprises:

and enabling the first pipeline to suck back air to form a first air column.
The method of claim 22, wherein the sample assembly further comprises a third conduit, the first conduit and the second conduit collectively communicating with the reaction cell via the third conduit, wherein "causing the first conduit and/or the second conduit to draw air back into the first air column" comprises:

and enabling the first pipeline or the second pipeline to suck back air to form a first air column.
The method of claim 22, wherein when performing a sample test, the method further comprises:

emptying the bottom liquid in the reaction tank;

flowing the reagent in the first line and/or the second line into the reaction cell, wherein the first gas column is eliminated during the flowing.