CN219758026U - Blood cell analyzer - Google Patents
Blood cell analyzer Download PDFInfo
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- CN219758026U CN219758026U CN202321187449.4U CN202321187449U CN219758026U CN 219758026 U CN219758026 U CN 219758026U CN 202321187449 U CN202321187449 U CN 202321187449U CN 219758026 U CN219758026 U CN 219758026U
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- storage tank
- valve
- blood cell
- sheath
- liquid
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- 210000000601 blood cell Anatomy 0.000 title claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 61
- 230000009471 action Effects 0.000 claims abstract description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 8
- 238000003860 storage Methods 0.000 claims description 74
- 239000012530 fluid Substances 0.000 claims description 42
- 238000001514 detection method Methods 0.000 claims description 39
- 230000003287 optical effect Effects 0.000 claims description 35
- 210000004027 cell Anatomy 0.000 claims description 14
- 239000002699 waste material Substances 0.000 claims description 14
- 238000004820 blood count Methods 0.000 claims description 7
- 210000003743 erythrocyte Anatomy 0.000 claims description 6
- 210000000265 leukocyte Anatomy 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 3
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 239000012898 sample dilution Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 30
- 238000000034 method Methods 0.000 description 8
- 239000006285 cell suspension Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000012488 sample solution Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000010437 gem Substances 0.000 description 2
- 229910001751 gemstone Inorganic materials 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present utility model relates to a blood cell analyzer comprising: a sample preparation device for preparing a first sample liquid and a second sample liquid containing a sample and a reagent; the control system is used for controlling the blood cell analyzer to complete each appointed action; the air source is used for generating positive and negative pressure; the air source is respectively connected with the positive pressure switching valve and the negative pressure switching valve. The blood cell analyzer provided by the utility model can not only provide stable sheath liquid, but also reduce the volume of the analyzer and the manufacturing cost of the analyzer, and has higher cost performance.
Description
Technical Field
The utility model relates to the field of blood cell analyzers, in particular to a blood cell analyzer.
Background
Five-class blood cell analyzers are used to analyze the presence of constituents in human blood, such as the counting of red blood cells in blood and the class detection of white blood cells.
The sheath flow technology can realize the differential detection of the white blood cells, and the specific method is that a capillary is used for aligning an optical flow chamber, and the cell suspension is ejected from the capillary. Simultaneously, the cell suspension and sheath fluid flowing out from the periphery flow through the sensitive area together, so that a single cell flow is formed in the middle of the cell suspension, and the periphery is surrounded by the sheath fluid. The sheath flow impedance technique also allows for red blood cell count detection by aligning a capillary with the gemstone aperture and ejecting the cell suspension from the capillary. Simultaneously, the cell suspension and sheath fluid flowing out from the periphery flow through the jewel hole together, so that the cell suspension forms single-arrangement cell flow in the middle, and the periphery is surrounded by the sheath fluid.
Both red cell count and white cell sorting generally require a stable sheath fluid to ensure reliable detection results. In the prior art, the following solutions for providing a stable sheath fluid are generally available:
(1) The air source is matched with a plurality of air storage tanks to form a plurality of stable positive pressures, and the stable positive pressures are used for driving the diluted liquid in different air storage tanks to form stable sheath liquid respectively. However, in this solution, the gas reservoir is relatively large, increasing the volume of the instrument.
(2) The diluent is pushed into the optical flow chamber or the sheath flow impedance cell at a constant speed by driving the injector to form stable sheath liquid. However, this solution is costly and is not suitable for low cost instruments.
Therefore, the above-described stable sheath fluid implementation is not suitable for low-cost and miniaturized five-class blood cell analyzers. .
Disclosure of Invention
In order to solve the above technical problems or at least partially solve the above technical problems, the present utility model provides a blood cell analyzer.
In a first aspect, the present utility model provides a blood cell analyzer comprising:
a sample preparation device for preparing a first sample liquid and a second sample liquid containing a sample and a reagent;
the control system is used for controlling the blood cell analyzer to complete each appointed action;
the air source is used for generating positive and negative pressure; the air source is respectively connected with the positive pressure switching valve and the negative pressure switching valve;
a first gas reservoir for storing an unstable positive pressure; the first gas storage tank is respectively connected with a one-way valve and a positive pressure switching valve;
a second gas reservoir for storing a stable positive pressure; the second gas storage tank is respectively connected with the one-way valve and the liquid storage tank;
the third gas storage tank is used for storing negative pressure; the third gas storage tank is respectively connected with a second pressure sensor and a negative pressure switching valve;
a check valve for preventing compressed air in the second air storage tank from reversely flowing back to the first air storage tank; the one-way valve is connected with the first gas storage tank and the second gas storage tank;
an optical detection device for performing white blood cell count detection; the optical detection device is respectively connected with the optical sheath liquid valve and the waste liquid collecting and treating device;
the sheath flow impedance detection device is used for performing erythrocyte counting detection; the sheath flow impedance detection device is respectively connected with the waste liquid collection and treatment device and the first sample flow driving device;
a reservoir for providing a stabilizing sheath fluid for the optical flow cell and the sheath flow impedance cell; the liquid storage tank is connected with the second gas storage tank;
a premix tank for diluting a sample to form a first sample solution; the premixing pool is connected with the waste liquid collecting and treating device;
a reaction cell for sample incubation to form a second sample fluid; the reaction tank is connected with a waste liquid collecting and treating device;
the first pressure sensor is used for detecting the pressure in the first gas storage tank; the first pressure sensor is connected with the first gas storage tank,
the second pressure sensor is used for detecting the pressure in the third gas storage tank; the second pressure sensor is connected with the third gas storage tank,
the second gas storage tank is connected with the liquid storage tank; the liquid storage tank is connected with the optical sheath liquid valve; the optical sheath liquid valve is connected with the optical detection device; the liquid storage tank is connected with a sheath flow resistance sheath liquid valve; the sheath flow resistance sheath liquid valve is connected with the sheath flow resistance detection device; the air source is connected with the positive pressure switching valve, the positive pressure switching valve is connected with the first air storage tank through a first pipeline, the first air storage tank is connected with the one-way valve through a second pipeline, and the second air storage tank is connected with the one-way valve; the air source is connected with a negative pressure switching valve, and the negative pressure switching valve is connected with a third air storage tank.
Preferably, the second gas reservoir is connected to the one-way valve by a third line.
Preferably, the air source is connected to the negative pressure switching valve through a fourth pipeline.
Preferably, the second gas reservoir is connected to the liquid reservoir by a fifth line.
Preferably, the reservoir is connected to the optical sheath fluid valve by a sixth conduit.
Preferably, the optical sheath fluid valve is connected to the optical detection device through a seventh pipeline.
Preferably, the reservoir is connected to the sheath flow impedance sheath fluid valve by an eighth conduit.
Preferably, the sheath fluid impedance sheath fluid valve is connected to the sheath fluid impedance detection device through a ninth pipeline.
Preferably, the air source is connected to the positive pressure switching valve through a tenth line.
Preferably, the negative pressure switching valve is connected to the third gas storage tank through an eleventh pipeline.
Compared with the prior art, the technical scheme provided by the embodiment of the utility model has the following advantages:
the blood cell analyzer provided by the utility model can not only provide stable sheath liquid, but also reduce the volume of the analyzer and the manufacturing cost of the analyzer, and has higher cost performance.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the utility model and together with the description, serve to explain the principles of the utility model.
In order to more clearly illustrate the embodiments of the utility model or the technical solutions of the prior art, the drawings which are used in the description of the embodiments or the prior art will be briefly described, and it will be obvious to a person skilled in the art that other drawings can be obtained from these drawings without inventive effort.
FIG. 1 is a schematic diagram of a five-class blood cell analyzer;
FIG. 2 is a schematic diagram of a blood cell analyzer according to the present utility model;
fig. 3 is a schematic diagram of sample sucking and distributing of a blood cell analyzer according to the present utility model.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Fig. 1 is a schematic diagram of a blood cell analyzer according to an embodiment of the present utility model.
The present utility model provides a blood cell analyzer, comprising:
a sample preparing section 101 for preparing a first sample solution and a second sample solution containing a sample and a reagent;
the control system is used for controlling the blood cell analyzer to complete each appointed action;
a gas source 102 for generating positive and negative pressure; the air source 102 is respectively connected with a positive pressure switching valve 115 and a negative pressure switching valve 116;
a first gas reservoir 103 for storing an unstable positive pressure; the first gas storage tank 103 is respectively connected with the one-way valve 105 and the positive pressure switching valve 115;
a second gas reservoir 104 for storing a stable positive pressure; the second gas storage tank 104 is respectively connected with the one-way valve 105 and the liquid storage tank 112;
a third air reservoir 111 for storing negative pressure; the third air reservoir 111 is connected to the second pressure sensor 110 and the negative pressure switching valve 116, respectively;
a check valve 105 for preventing compressed air in the second air storage tank 104 from flowing back to the first air storage tank 103; the check valve 105 connects the first gas storage tank 103 with the second gas storage tank 104;
an optical detection device 106 for performing white blood cell count detection; the optical detection device 106 is respectively connected with the optical sheath fluid valve 120 and the waste liquid collecting and processing device 123;
sheath flow impedance detection means 107 for performing red blood cell count detection; the sheath flow impedance detecting means 107 is connected to the waste liquid collecting and treating means 123 and the first sample flow driving means 124, respectively;
a reservoir 112 for providing a stabilizing sheath fluid for the optical flow cell and the sheath flow impedance cell; reservoir 112 is connected to second reservoir 104;
a premix tank 113 for sample dilution to form a first sample solution; the premixing pool 113 is connected with a waste liquid collecting and treating device 123;
a reaction cell 114 for sample incubation reaction to form a second sample liquid; the reaction tank 114 is connected with a waste liquid collecting and treating device 123;
a first pressure sensor 109 for detecting the pressure in the first gas storage 103; the first pressure sensor 109 is connected to the first gas reservoir 103,
a second pressure sensor 110 for detecting the pressure in the third gas storage tank 111; the second pressure sensor 110 is connected to the third gas storage tank 111,
the second gas reservoir 104 is connected to the liquid reservoir 112; reservoir 112 is connected to optical sheath valve 120; the optical sheath fluid valve 120 is connected with the optical detection device 106; reservoir 112 is connected to sheath fluid valve 118; the sheath fluid valve 118 is connected with the sheath fluid impedance detecting device 107; the air source 102 is connected with the positive pressure switching valve 115, the positive pressure switching valve 115 is connected with the first air storage tank 103 through a first pipeline 201, the first air storage tank 103 is connected with the one-way valve 105 through a second pipeline 202, and the second air storage tank 104 is connected with the one-way valve 105; the air source 102 is connected to a negative pressure switching valve 116, and the negative pressure switching valve 116 is connected to the third air reservoir 111.
In the embodiment of the present utility model, the second gas storage 104 is connected to the check valve 105 through the third pipeline 203.
In an embodiment of the present utility model, the air source 102 is connected to the negative pressure switching valve 116 through a fourth line 204.
In an embodiment of the present utility model, the second gas storage 104 is connected to the liquid storage 112 through a fifth pipeline 205.
In an embodiment of the present utility model, reservoir 112 is connected to sheath valve 120 via sixth conduit 206.
In the embodiment of the present utility model, the optical sheath fluid valve 120 is connected to the optical detection device 106 through a seventh pipe 207.
In an embodiment of the present utility model, reservoir 112 is connected to sheath fluid valve 118 via eighth conduit 208.
In an embodiment of the present utility model, the sheath fluid valve 118 is connected to the sheath fluid impedance detecting means 107 through a ninth conduit 209.
In an embodiment of the present utility model, gas source 102 is connected to positive pressure switch valve 115 via tenth conduit 210.
In the embodiment of the present utility model, the negative pressure switching valve 116 is connected to the third air reservoir 111 through the eleventh pipe 211.
In a specific embodiment, the detection flow of the blood cell analyzer provided by the utility model is as follows: under the action of the sample preparation device 101, the sample to be tested in the test tube is sucked. The sample to be measured in the sample preparation device 101 is injected into the premix tank 113, and the first reagent in the reagent supply device 121 is injected into the premix tank 113 to be mixed with the sample to be measured for reaction to generate a first sample solution.
The sample to be measured in the sample preparation device 101 is injected into the reaction tank 114, and the second reagent in the reagent supply device 121 is injected into the reaction tank 114 to be mixed with the sample to be measured for reaction to generate a second sample liquid.
The first sample liquid in the premixing pool 113 is sent into the twelfth pipeline 212 through the waste liquid collecting and processing device 123, and the first sample liquid in the twelfth pipeline 212 is pushed by the 124; the sheath flow impedance sheath fluid valve 118 is opened, the diluent in the liquid storage tank 112 enters the sheath flow impedance detection device 107 to form stable sheath fluid, and the stable sheath fluid wraps the first sample fluid to perform red blood cell counting detection in the sheath flow impedance detection device 107.
The second sample liquid in the reaction tank 114 is sent into the thirteenth pipeline 213 through the waste liquid collecting and processing device 123, and the second sample liquid in the thirteenth pipeline 213 is pushed by the second sample flow driving device 125; the optical sheath fluid valve 120 is opened, the diluent in the liquid storage tank 112 enters the optical detection device 106 to form stable sheath fluid, and the stable sheath fluid wraps the second sample fluid to perform leukocyte classification detection in the optical detection device 106.
After the detection, the liquid in the cleaning device 122 is driven to clean the premixing tank 113/the reaction tank 114.
In a specific embodiment, the pressure building flow of the blood cell analyzer provided by the utility model is as follows:
in the TO time, controlling the air source 102 and the positive pressure switching valve 115, and starting the air source 102 and the positive pressure switching valve 115 when the first pressure sensor 109 detects that the pressure in the first air storage tank 103 is smaller than a first set pressure; when the first pressure sensor 109 detects that the pressure in the first gas storage tank 103 reaches the second set pressure, the positive pressure switching valve 115 is closed;
in the time T1, controlling the air source 102 and the positive pressure switching valve 115, and starting the air source 102 and the positive pressure switching valve 115 when the first pressure sensor 109 detects that the pressure in the first air storage tank 103 is smaller than the third set pressure; when the first pressure sensor 109 detects that the pressure in the first gas storage tank 103 reaches the fourth set pressure, the positive pressure switching valve 115 is closed;
and the controller is used for controlling the flow.
During time T0, the sheath fluid impedance sheath fluid valve 118 and the optical sheath fluid valve 120 are all in a closed state.
Wherein the first set pressure is greater than the fourth set pressure.
In the time T1, the pressure in the second gas storage tank 104 is larger than the pressure in the first gas storage tank 103, and the pressure in the second gas storage tank 104 is larger than the fourth set pressure.
In the time T1, the pressure change in the second gas storage tank 104 is less than or equal to 10kPa; the volume of the first gas storage tank 103 is less than or equal to 300mL, and the volume of the second gas storage tank 104 is less than or equal to 300mL; the air source 102 has an empty flow rate of > 3L/min.
The blood cell analyzer provided by the utility model can not only provide stable sheath liquid, but also reduce the volume of the analyzer and the manufacturing cost of the analyzer, and has higher cost performance.
It should be noted that in this document, relational terms such as "first" and "second" and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The foregoing is only a specific embodiment of the utility model to enable those skilled in the art to understand or practice the utility model. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present utility model is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A blood cell analyzer, comprising:
a sample preparing unit (101) for preparing a first sample liquid and a second sample liquid containing a sample and a reagent;
the control system is used for controlling the blood cell analyzer to complete each appointed action;
a gas source (102) for generating positive and negative pressure; the air source (102) is respectively connected with a positive pressure switching valve (115) and a negative pressure switching valve (116);
a first gas reservoir (103) for storing an unstable positive pressure; the first gas storage tank (103) is respectively connected with the one-way valve (105) and the positive pressure switching valve (115);
a second gas reservoir (104) for storing a stable positive pressure; the second gas storage tank (104) is respectively connected with the one-way valve (105) and the liquid storage tank (112);
a third gas storage tank (111) for storing negative pressure; the third gas storage tank (111) is respectively connected with the second pressure sensor (110) and the negative pressure switching valve (116);
a one-way valve (105) for preventing compressed air in the second air reservoir (104) from flowing back into the first air reservoir (103) in a reverse direction; the one-way valve (105) is connected with the first gas storage tank (103) and the second gas storage tank (104);
an optical detection device (106) for performing white blood cell count detection; the optical detection device (106) is respectively connected with the optical sheath liquid valve (120) and the waste liquid collecting and treating device (123);
sheath flow impedance detection means (107) for performing a red blood cell count detection; the sheath flow impedance detection device (107) is respectively connected with the waste liquid collection and treatment device (123) and the first sample flow driving device (124);
a reservoir (112) for providing a stabilizing sheath fluid to the optical flow cell and the sheath flow impedance cell; the liquid storage tank (112) is connected with the second gas storage tank (104);
a premix tank (113) for sample dilution to form a first sample liquid; the premixing pool (113) is connected with a waste liquid collecting and treating device (123);
a reaction cell (114) for a sample incubation reaction to form a second sample liquid; the reaction tank (114) is connected with a waste liquid collecting and treating device (123);
a first pressure sensor (109) for detecting the pressure in the first gas reservoir (103); the first pressure sensor (109) is connected with the first gas storage tank (103),
a second pressure sensor (110) for detecting the pressure in the third gas storage tank (111); the second pressure sensor (110) is connected with the third gas storage tank (111),
the second gas storage tank (104) is connected with the liquid storage tank (112); the liquid storage tank (112) is connected with the optical sheath liquid valve (120); the optical sheath liquid valve (120) is connected with the optical detection device (106); the liquid storage pool (112) is connected with a sheath flow resistance sheath liquid valve (118); the sheath flow resistance sheath liquid valve (118) is connected with the sheath flow resistance detection device (107); the air source (102) is connected with the positive pressure switching valve (115), the positive pressure switching valve (115) is connected with the first air storage tank (103) through a first pipeline (201), the first air storage tank (103) is connected with the one-way valve (105) through a second pipeline (202), and the second air storage tank (104) is connected with the one-way valve (105); the air source (102) is connected with a negative pressure switching valve (116), and the negative pressure switching valve (116) is connected with a third air storage tank (111).
2. A blood cell analyzer according to claim 1, characterized in that the second gas reservoir (104) is connected to the non-return valve (105) via a third line (203).
3. The blood cell analyzer of claim 1, wherein the gas source (102) is connected to the negative pressure switching valve (116) via a fourth line (204).
4. A blood cell analyzer according to claim 1, wherein the second gas reservoir (104) is connected to the reservoir (112) by a fifth conduit (205).
5. The blood cell analyzer of claim 1, wherein the reservoir (112) is connected to the optical sheath fluid valve (120) by a sixth conduit (206).
6. The blood cell analyzer according to claim 1, characterized in that the optical sheath fluid valve (120) is connected to the optical detection device (106) via a seventh line (207).
7. The blood cell analyzer of claim 1, wherein the reservoir (112) is connected to the sheath flow impedance sheath valve (118) by an eighth conduit (208).
8. A blood cell analyzer according to claim 1, characterized in that the sheath fluid impedance sheath fluid valve (118) is connected to the sheath fluid impedance detection means (107) via a ninth conduit (209).
9. The blood cell analyzer of claim 1, wherein the gas source (102) is connected to the positive pressure switching valve (115) via a tenth conduit (210).
10. The blood cell analyzer according to claim 1, wherein the negative pressure switching valve (116) is connected to the third gas reservoir (111) through an eleventh line (211).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321187449.4U CN219758026U (en) | 2023-05-16 | 2023-05-16 | Blood cell analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321187449.4U CN219758026U (en) | 2023-05-16 | 2023-05-16 | Blood cell analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219758026U true CN219758026U (en) | 2023-09-26 |
Family
ID=88084622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321187449.4U Active CN219758026U (en) | 2023-05-16 | 2023-05-16 | Blood cell analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219758026U (en) |
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2023
- 2023-05-16 CN CN202321187449.4U patent/CN219758026U/en active Active
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