CN116256528A - Sample analyzer - Google Patents
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- CN116256528A CN116256528A CN202310035505.0A CN202310035505A CN116256528A CN 116256528 A CN116256528 A CN 116256528A CN 202310035505 A CN202310035505 A CN 202310035505A CN 116256528 A CN116256528 A CN 116256528A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
- G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
- G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1004—Cleaning sample transfer devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a sample analyzer, which comprises a liquid storage mechanism, a detection mechanism and a distribution mechanism, wherein the liquid storage mechanism comprises a first reaction tank and a liquid storage tank which are communicated with each other, the detection mechanism comprises a first detection component which is communicated with the first reaction tank and detects liquid to be detected in the first reaction tank, the distribution mechanism comprises a positive pressure component, a first driving component, a sampling component, a second driving component, a switching component and a waste discharge driving component, the positive pressure component is communicated with the liquid storage tank and drives diluent in the liquid storage tank into the first reaction tank, the first driving component drives the sampling component to take samples into the first reaction tank, the second driving component is matched with the switching component to drive the liquid to be detected in the first reaction tank into the first detection component, and the second driving component drives diluent in the liquid storage tank into the first detection component which is communicated with the waste discharge driving component. The sample analyzer of the invention does not need a plurality of injectors, and has high liquid adding efficiency and low cost.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a sample analyzer.
Background
In the existing analyzers, whether the detection of WBC/BASO/DIFF is realized by a chemical method or the detection of RBC/PLT is realized by an impedance method, a sample is required to be added into a reaction tank for optical detection. After the optical detection is completed, the waste liquid outlet of the optical detection is usually required to be communicated with the reaction tank or the negative pressure chamber, a liquid path system of the optical detection is usually used for injecting a cleaning reagent into the reaction tank for cleaning, and in one test period, before the reagent needs to be used, the injector needs to have a back suction time, the liquid adding efficiency is low, and in the prior art, a plurality of injectors are often required to be respectively arranged for improving the liquid adding efficiency, but the cost is too high.
In view of the above-described drawbacks, it is necessary to provide a new sample analyzer.
Disclosure of Invention
The invention mainly aims to provide a sample analyzer, which aims to solve the problem that the cost is too high due to the fact that a plurality of injectors are added to improve the liquid adding efficiency in the existing analyzer.
To achieve the above object, the present invention provides a sample analyzer comprising:
the liquid storage mechanism comprises a first reaction tank and a liquid storage tank for containing diluent, and the first reaction tank is communicated with the liquid storage tank through a pipeline;
the detection mechanism comprises a first detection component communicated with the first reaction tank through a buffer pipeline, and the first detection component is used for detecting the liquid to be detected in the first reaction tank;
the distribution mechanism comprises a positive pressure component, a first driving component, a sampling component, a second driving component, a switching component and a waste discharge driving component, wherein the positive pressure component is communicated with the liquid storage tank and used for driving diluent in the liquid storage tank to the first reaction tank, the first driving component is used for driving the sampling component to take samples into the first reaction tank, the second driving component is matched with the switching component to drive liquid to be detected in the first reaction tank to the first detection component, the second driving component is used for driving diluent in the liquid storage tank to the first detection component, a liquid outlet of the first detection component, a waste discharge outlet of the first reaction tank are communicated with the waste discharge driving component, and the waste discharge driving component is used for driving waste liquid in the first detection component to be discharged from the liquid outlet and used for driving waste liquid in the first reaction tank to be discharged from the waste discharge outlet.
Preferably, the positive pressure assembly comprises a positive pressure tank in communication with the reservoir and a gas drive in communication with the positive pressure tank.
Preferably, the distribution mechanism further comprises an atmospheric pressure component, and the liquid outlet of the first detection component is communicated with the waste discharge driving piece through the atmospheric pressure component.
Preferably, the switching component comprises an on-off piece arranged on a pipeline between the first reaction tank and the first detection component, the second driving component comprises a second driving piece and a third driving piece, and the on-off piece is used for communicating the buffer pipeline with the first reaction tank when being in a communicating state or is used for disconnecting the buffer pipeline with the first reaction tank when being in a disconnecting state; the second driving piece and the third driving piece are used for driving the liquid to be detected in the first reaction tank into the buffer pipeline together when the on-off piece is in a communication state, driving the liquid to be detected in the buffer pipeline into the first detection assembly together when the on-off piece is in an off state, and the second driving piece is also used for driving the diluent in the liquid storage tank into the first detection assembly.
Preferably, the second drive member and the third drive member are both syringes and the second drive assembly is a duplex syringe assembly.
Preferably, the first detection component is an optical detection module, which is used for performing DIFF detection and BASO detection on the liquid to be detected in the first detection component.
Preferably, the first drive assembly comprises a fourth drive for driving the sampling assembly to take a sample into the first reaction cell.
Preferably, the liquid storage mechanism further comprises a second reaction tank, the second reaction tank is communicated with the liquid storage tank through a pipeline, the first driving component further comprises a first driving piece, the first driving piece is used for driving the diluent in the liquid storage tank into the second reaction tank, and the positive pressure component is further used for driving the diluent in the liquid storage tank into the second reaction tank; the detection mechanism further comprises a second detection component communicated with the second reaction tank through a pipeline, the second detection component is used for detecting liquid to be detected in the second reaction tank, and the distribution mechanism further comprises a negative pressure component communicated with a liquid outlet of the second detection component.
Preferably, the first driving member and the fourth driving member are both syringes.
Preferably, the second detection component is an impedance detection module, and is configured to perform RBC detection on the liquid to be detected in the second reaction tank.
Preferably, the waste outlet of the first reaction tank and the waste outlet of the second reaction tank are both communicated with the positive pressure assembly, and the positive pressure assembly is further used for injecting gas into the first reaction tank through the waste outlet of the first reaction tank and injecting gas into the second reaction tank through the waste outlet of the second reaction tank.
Preferably, the sample analyzer further comprises a reagent quantifying mechanism for adding a reagent and a sample to the first reaction cell and the second reaction cell.
According to the technical scheme, the first driving component and the second driving component both adopt the syringe component, the positive pressure component is used as a positive pressure source to provide positive pressure power, the syringe with a constant current source is used for providing reagents under the scene of high quantitative precision requirement by adopting a liquid supply scheme of the positive pressure source and the syringe, the positive pressure component with the constant voltage source is used for providing reagents in the cleaning link with low quantitative precision requirement, namely the positive pressure component is used for providing a cleaning liquid power source for the sample analyzer, in a word, the positive pressure source and the syringe are used in a matched manner, and different actions such as cleaning, sample liquid pushing and the like are completed, the syringe and the positive pressure component can be distributed through a liquid path design, and the parallelism of the two actions is realized without waiting or adding a new syringe or power source. The sample analyzer of the invention does not need a plurality of injectors, can save injector resources and has high liquid adding efficiency, thereby reducing the cost on the whole design, improving the detection speed and shortening the measurement period.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to the structures shown in these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a sample analyzer according to an embodiment of the invention.
Reference numerals illustrate:
the achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present invention are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.
Furthermore, descriptions such as those referred to as "first," "second," and the like, are provided for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implying an order of magnitude of the indicated technical features in the present disclosure. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.
In the present invention, unless explicitly specified and limited otherwise, the terms "connected," "fixed," and the like are to be construed broadly, and for example, "connected" may be either a fixed connection or a removable connection or integrated; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
In addition, the technical solutions of the embodiments of the present invention may be combined with each other, but it is necessary to be based on the fact that those skilled in the art can implement the technical solutions, and when the technical solutions are contradictory or cannot be implemented, the combination of the technical solutions should be considered as not existing, and not falling within the scope of protection claimed by the present invention.
The invention provides a sample analyzer, and aims to solve the problem of excessive cost caused by adding a plurality of syringes for improving the liquid adding efficiency in the existing analyzer.
Referring to fig. 1, the sample analyzer includes a liquid storage mechanism, a detection mechanism and a distribution mechanism, the liquid storage mechanism includes a first reaction tank 11 and a liquid storage tank 13 for containing a diluent, the first reaction tank 11 is communicated with the liquid storage tank 13 through a pipeline, the detection mechanism includes a first detection component 21 communicated with the first reaction tank 11 through a buffer pipeline, the first detection component 21 is used for detecting a liquid to be detected in the first reaction tank 11, the distribution mechanism includes a positive pressure component 31, a first driving component 32, a sampling component 33, a second driving component 34, a switching component 35 and a waste liquid discharge driving member 36, the positive pressure component 31 is communicated with the liquid storage tank 13 and is used for driving the diluent in the liquid storage tank 13 into the first reaction tank 11, the first driving component 32 is used for driving the sampling component 33 to take the sample into the first reaction tank 11, the second driving component 34 is matched with the switching component 35 to drive the liquid to be detected in the first detection component 21, the second driving component 34 is used for driving the diluent in the first reaction tank 11 into the first detection component 21, the first driving component 36 is communicated with the waste liquid discharge member 36 and the waste liquid from the first reaction tank 11, and the waste liquid discharge member is communicated with the waste liquid discharge member 11.
In the sample analyzer of the invention, the first driving component 32 and the second driving component 34 both adopt the injector component, the positive pressure component 31 is used as the positive pressure source to provide positive pressure power, the solution supply scheme of the positive pressure source and the injector is adopted, the injector of the constant current source is used for providing reagent under the scene of high quantitative precision requirement, the positive pressure component 31 of the constant voltage source is used for providing reagent in the cleaning link of low quantitative precision requirement, namely the positive pressure component 31 provides the cleaning solution power source for the sample analyzer, in a word, the positive pressure source and the injector are used in a matched way, and then different actions such as cleaning, sample pushing and the like are completed, the injector and the positive pressure component 31 can be distributed through the design of a solution path, and the parallelism of the two actions is realized without waiting or adding a new injector or power source. The sample analyzer of the invention does not need a plurality of injectors, can save injector resources and has high liquid adding efficiency, thereby reducing the cost on the whole design, improving the detection speed and shortening the measurement period.
Referring specifically to fig. 1, V01 to V19 are switching elements in the switching element 35. Before starting the test, the blood sample is collected and distributed, and the reagent immersed in the first reaction tank 11 after the last detection is completed is removed by the waste discharge driving member 36 (waste liquid pump waste discharge module in the figure) and the switching member V13. The measurement procedure is started, the sampling needle is driven to suck the sample by controlling the first driving component 32, the sampling needle is moved and the sample is distributed to the first reaction tank 11, and the hemolytic agent is quantitatively distributed to the first reaction tank 11 through the hemolytic agent quantitative device.
After sample distribution is completed, the positive pressure pushing diluent formed by the positive pressure component 31 is switched between V04 and V07 to complete cleaning of the inner wall of the sample needle, and normal pressure cleaning is realized. If desired, such as when whole blood is to be washed from the remaining sample needle after sample dispensing, the first drive member 322 of the first drive assembly 32 may be used to provide power to effect high pressure washing. After the addition of the sample and the reagent is completed, the V18 is switched, and a PC positive pressure source (Bubble-1) can be used for pushing gas to the first reaction tank 11, so that the first reaction tank 11 is uniformly mixed with bubbles, and the sample preparation is completed.
It should be noted that, the cleaning of the inner wall of the sample needle is provided with two power sources, which can realize the conventional cleaning through the positive pressure assembly 31, and can also realize the high pressure cleaning through the first driving assembly 32 and the positive pressure assembly 31. In addition, the liquid storage tank 13 is always connected with positive pressure, so that the precipitation of dissolved gas in the diluent can be effectively avoided, and the DIL entering optics does not need to be filtered independently.
Wherein, in an embodiment, positive pressure assembly 31 comprises a positive pressure tank 311 and a gas drive 312, positive pressure tank 311 is in communication with reservoir 13, and gas drive 312 is in communication with positive pressure tank 311. The liquid inlet of the liquid storage tank 13 is communicated with a power source of the liquid pump of the instrument, the air inlet is communicated with the positive pressure tank 311 in a normal state, and the liquid outlet is communicated with the cleaning component or the injector through a switching piece. The air inlet of the positive pressure tank 311 is communicated with the air pump through a switching piece to establish positive pressure, and the air outlet of the positive pressure tank 311 is communicated with the normal pressure tank through the liquid storage tank 13 and a throttling piece to realize pressure release and adjustment. The positive pressure source is established in the sample analyzer, the positive pressure establishing process is to start the air pumps LP and V01, the pressure value in the air chamber reaches a pressure value slightly larger than a preset value through the pressure monitoring component, and when the pressure value exceeds the preset value, the pressure value is released through V17 switching to reach the preset value. And the positive pressure source air pump has small flow requirement and low cost.
Further, the distribution mechanism comprises an atmospheric pressure assembly 37, and the liquid outlet of the first detection assembly 21 is communicated with the waste discharge driving piece 36 through the atmospheric pressure assembly 37. The normal pressure chamber is introduced as a liquid storage device for temporarily collecting optical detection waste liquid, an optical detection waste liquid outlet is connected with the normal pressure tank NC but not connected with the reaction tank, the first reaction tank 11 does not need to wait for the optical detection to finish, the starting time is completely determined by the reaction condition, the sample liquid to be tested for the next sample test can be prepared in advance in an iterative manner, the design is simplified, and the total flow time is saved. And moreover, the liquid outlet of the optical detection module is communicated with the normal pressure tank, other resources or action influences are not considered, the stability of the outlet pressure is favorable for the stability of the optical sheath flow, and the accuracy of the detection result is improved.
In addition, in an embodiment, the switching assembly 35 includes an on-off member 351 provided on the pipe between the first reaction tank 11 and the first detection assembly 21, the second driving assembly 34 includes a second driving member 341 and a third driving member 342, and the on-off member 351 is used to connect the buffer pipe and the first reaction tank 11 when in the connected state, or to disconnect the buffer pipe and the first reaction tank 11 when in the disconnected state; the second driving piece 341 and the third driving piece 342 are used for driving the liquid to be tested in the first reaction tank 11 into the buffer pipeline together when the on-off piece 351 is in a connected state, and driving the liquid to be tested in the buffer pipeline into the first detection assembly 21 together when the on-off piece 351 is in an disconnected state, and the second driving piece 341 is also used for driving the diluent in the liquid storage tank 13 into the first detection assembly 21. In the preparation and detection of the optical detection sample, the switching V08 and the on-off member 351 are simultaneously operated by the second driving member 341 and the third driving member 342 of the second driving assembly 34, and the prepared sample liquid after being uniformly mixed is transferred from the first reaction tank 11 to the pipeline between the on-off member 351 and the flow chamber of the first detection assembly 21 and the third driving member 342, so as to complete the preparation of the optical detection sample. Then, the on-off member 351 is closed, the V11 is switched, the diluent is pushed by the second driving member 341 to form a sheath liquid, the sample liquid is pushed by the third driving member 342 to enter the flow chamber of the first detection assembly 21 through the sample needle, the sheath liquid wraps the sample liquid to flow through the optical detection device of the first detection assembly 21 for detection, and during the period, the V10 is switched to collect the waste liquid generated by detection into the normal pressure tank NC. The step is to prepare DIFF and BASO sample solutions in two reaction tanks respectively, and then to convey the sample solutions to an optical detection position. Wherein, valve V16 one end is being connected liquid storage pot 13, and V08 is being connected to one end, V08 and first reaction tank 11 intercommunication, open V16, V08 can be through the liquid release of positive pressure drive liquid storage pot 13 entering first reaction tank 11, in the DIFF testing process, can wash first reaction tank 11 through switching V16 and V08 to through the positive pressure drive diluent that positive pressure subassembly 31 produced, need not to wait for second driving piece 341 to accomplish the sample testing process and push away the appearance, can carry out the reaction tank in advance and wash, realize parallel improvement speed. After DIFF optical detection is completed, V16 and on-off member 351 are switched, and the diluent is driven by positive pressure generated by positive pressure assembly 31 to cooperate with second driving member 341 to complete cleaning of the optical detection device and the pipeline. Wherein the on-off member 351 may be a pressure break valve.
Wherein, the second driving member 341 and the third driving member 342 are both syringes, and the second driving assembly 34 is a duplex syringe assembly. The syringe can not only push a sample, a reagent, etc., but also more accurately meter the sample, the reagent, etc.
Further, to achieve multiple functions, the first detection component 21 is an optical detection module, which is used for performing DIFF detection and BASO detection on the liquid to be detected in the first detection component 21. The quantitative operations such as the transfer of the liquid to be measured are completed by the second driving member 341 and the third driving member 342, and the detection of the liquid to be measured by the first detecting assembly 21 includes, but is not limited to, DIFF detection and BASO detection. In the prior art, since the outlet end of the optical detection needs to be communicated with the waste liquid tank or the reaction tank, and the like, the pressure of the negative pressure chamber used for the connection of the outlet end with the waste liquid tank or the impedance detection is unstable in the pressure establishing process, and the optical detection can be affected, so that the outlet end of the first detection component 21 is not connected with the negative pressure chamber, and the problem can be effectively avoided. The application links to each other with optical detection waste liquid export through ordinary pressure subassembly 37, ordinary pressure room NC for optical detection waste liquid export does not communicate with the negative pressure room, so the negative pressure room builds the pressure time and need not consider the optical outlet state, can make to build the pressure time nimble, simplifies the design.
In addition, in the above-described embodiment, the first driving assembly 32 includes the fourth driving member 321, and the fourth driving member 321 is used to drive the sampling assembly 33 to take the sample into the first reaction cell 11. The measurement flow is started, the fourth driving piece 321 is controlled to drive the sampling needle to suck the sample, the sampling needle is moved and the sample is distributed to the first reaction tank 11, and the hemolytic agent is quantitatively distributed to the first reaction tank 11 through the hemolytic agent quantitative device.
In an embodiment, the liquid storage mechanism further comprises a second reaction tank 12, the second reaction tank 12 is communicated with the liquid storage tank 13 through a pipeline, the first driving component 32 further comprises a first driving component 322, the first driving component 322 is used for driving the diluent in the liquid storage tank 13 into the second reaction tank 12, and the positive pressure component 31 is further used for driving the diluent in the liquid storage tank 13 into the second reaction tank 12; the detection mechanism further comprises a second detection component 22 communicated with the second reaction tank 12 through a pipeline, the second detection component 22 is used for detecting liquid to be detected in the second reaction tank 12, and the distribution mechanism further comprises a negative pressure component 38 communicated with a liquid outlet of the second detection component 22. The impedance detection sample preparation and detection process is that a fourth driving piece 321 in the first driving component 32 is controlled to drive a sampling needle to absorb a sample, the sampling needle is moved and the sample is distributed to the second reaction tank 12, a hemolysis agent quantitative device is used for distributing a quantitative hemolysis agent to the second reaction tank 12, a first driving piece 322 of the first driving component 32 is used for distributing a quantitative diluent to the second reaction tank 12, and after the sample and the reagent are added, a V19 is switched, so that a PC positive pressure source (buffer-2) can be used for pushing gas to the second reaction tank 12, and Bubble mixing of the second reaction tank 12 is completed, thereby completing sample preparation. In the preparation process, the pressure is built in the negative pressure chamber through the liquid pump, and after the sample preparation is completed in the reaction tank of the impedance detection module, the impedance detection is completed through the shutdown pressure of the negative pressure chamber.
The negative pressure assembly 38 includes a negative pressure tank that is directly pressurized by the liquid pump and communicates with the second reaction cell 12 for the impedance channel of the second detection assembly 22 to provide negative pressure for the counting process. The negative pressure source is established in the sample analyzer, and the negative pressure establishing process is to start the waste liquid pump assembly and the V03, and the pressure of the air chamber reaches a preset value through the pressure monitoring assembly. The negative pressure chamber pressure building or impedance counting stage and the optical detection stage can be arranged at will without constraint. Furthermore, the reagent immersed in the second reaction tank 12 after the end of the previous detection is removed by the waste discharge driving member 36 (waste pump waste discharge module in fig. 1) and V14 before the impedance detection.
Further, the first driving member 322 and the fourth driving member 321 are both syringes. The syringe can not only push a sample, a reagent, etc., but also more accurately meter the sample, the reagent, etc.
In addition, the second detection component 22 is an impedance detection module, and is configured to perform RBC detection on the liquid to be detected in the second reaction tank 12. The second reaction tank 12 is used for preparing a sample liquid to be detected for RBC detection, and the sample liquid passes through the second detection assembly 22 after being prepared, and negative pressure is provided through a negative pressure chamber to realize detection.
Further, the waste outlet of the first reaction tank 11 and the waste outlet of the second reaction tank 12 are both communicated with the positive pressure component 31, and the positive pressure component 31 is further used for injecting gas into the first reaction tank 11 through the waste outlet of the first reaction tank 11 and injecting gas into the second reaction tank 12 through the waste outlet of the second reaction tank 12. The first reaction tank 11 and the second reaction tank 12 are cleaned, the cleaning is realized by driving the liquid in the liquid storage tank 13 to the first reaction tank 11 and the second reaction tank 12 by the positive pressure component 31, the mixing of the sample and the reagent is realized by providing positive pressure for bubbling through the positive pressure chamber, namely, the positive pressure component 31 is a bubble mixing generation source, so that the positive pressure component 31 can be used for cleaning the first reaction tank 11 and the second reaction tank 12, and the positive pressure component 31 is used for bubbling and mixing the sample in the first reaction tank 11 and the second reaction tank 12, and the positive pressure component 31 is fully utilized, so that the resource utilization rate is improved.
The sample analyzer further includes a reagent quantitative mechanism for adding a reagent and a sample to the first reaction cell 11 and the second reaction cell 12. The reagent quantifying mechanism comprises a syringe, a sample needle and a valve, and is used for sucking and distributing a sample, adding a reagent and the like.
The foregoing description is only of the preferred embodiments of the present invention and is not intended to limit the scope of the invention, and all equivalent structural changes made by the description of the present invention and the accompanying drawings or direct/indirect application in other related technical fields are included in the scope of the invention.
Claims (12)
1. A sample analyzer, comprising:
the liquid storage mechanism comprises a first reaction tank and a liquid storage tank for containing diluent, and the first reaction tank is communicated with the liquid storage tank through a pipeline;
the detection mechanism comprises a first detection component communicated with the first reaction tank through a buffer pipeline, and the first detection component is used for detecting the liquid to be detected in the first reaction tank;
the distribution mechanism comprises a positive pressure component, a first driving component, a sampling component, a second driving component, a switching component and a waste discharge driving component, wherein the positive pressure component is communicated with the liquid storage tank and used for driving diluent in the liquid storage tank to the first reaction tank, the first driving component is used for driving the sampling component to take samples into the first reaction tank, the second driving component is matched with the switching component to drive liquid to be detected in the first reaction tank to the first detection component, the second driving component is used for driving diluent in the liquid storage tank to the first detection component, a liquid outlet of the first detection component and a waste discharge outlet of the first reaction tank are communicated with the waste discharge driving component, and the waste discharge driving component is used for driving waste liquid in the first detection component to be discharged from the liquid outlet and used for driving waste liquid in the first reaction tank to be discharged from the waste outlet.
2. The sample analyzer of claim 1, wherein the positive pressure assembly comprises a positive pressure tank in communication with the reservoir and a gas drive in communication with the positive pressure tank.
3. The sample analyzer of claim 1, wherein the dispensing mechanism further comprises an atmospheric pressure assembly, wherein the liquid outlet of the first detection assembly is in communication with the waste drive member via the atmospheric pressure assembly.
4. The sample analyzer of claim 1, wherein the switching assembly comprises an on-off member provided on the conduit between the first reaction cell and the first detection assembly, the second drive assembly comprising a second drive member and a third drive member, the on-off member for communicating the buffer conduit with the first reaction cell when in a connected state or for disconnecting the buffer conduit with the first reaction cell when in a disconnected state; the second driving piece and the third driving piece are used for driving the liquid to be detected in the first reaction tank into the buffer pipeline together when the on-off piece is in a communication state, driving the liquid to be detected in the buffer pipeline into the first detection assembly together when the on-off piece is in an off state, and the second driving piece is also used for driving the diluent in the liquid storage tank into the first detection assembly.
5. The sample analyzer of claim 4, wherein the second drive member and the third drive member are each a syringe and the second drive assembly is a duplex syringe assembly.
6. The sample analyzer of any of claims 1-5, wherein the first detection component is an optical detection module for performing DIFF detection and BASO detection of the fluid under test within the first detection component.
7. The sample analyzer of any of claims 1-5, wherein the first drive assembly comprises a fourth drive for driving the sampling assembly to take a sample into the first reaction cell.
8. The sample analyzer of claim 7, wherein the reservoir mechanism further comprises a second reaction cell in communication with the reservoir via a conduit, the first drive assembly further comprising a first drive for driving diluent from the reservoir into the second reaction cell, the positive pressure assembly further for driving diluent from the reservoir into the second reaction cell; the detection mechanism further comprises a second detection component communicated with the second reaction tank through a pipeline, the second detection component is used for detecting liquid to be detected in the second reaction tank, and the distribution mechanism further comprises a negative pressure component communicated with a liquid outlet of the second detection component.
9. The sample analyzer of claim 8, wherein the first drive member and the fourth drive member are each syringes.
10. The sample analyzer of claim 8, wherein the second detection component is an impedance detection module for performing RBC detection on the fluid under test in the second reaction cell.
11. The sample analyzer of claim 8, wherein the waste outlet of the first reaction cell and the waste outlet of the second reaction cell are both in communication with the positive pressure assembly, the positive pressure assembly further configured to inject gas into the first reaction cell through the waste outlet of the first reaction cell and to inject gas into the second reaction cell through the waste outlet of the second reaction cell.
12. The sample analyzer of claim 8, further comprising a reagent quantification mechanism for adding reagents and samples to the first reaction cell and the second reaction cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310035505.0A CN116256528A (en) | 2023-01-10 | 2023-01-10 | Sample analyzer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310035505.0A CN116256528A (en) | 2023-01-10 | 2023-01-10 | Sample analyzer |
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| CN116256528A true CN116256528A (en) | 2023-06-13 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117169513A (en) * | 2023-09-04 | 2023-12-05 | 中元汇吉生物技术股份有限公司 | Specific protein detection and analysis method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117169513A (en) * | 2023-09-04 | 2023-12-05 | 中元汇吉生物技术股份有限公司 | Specific protein detection and analysis method |
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