CN212410610U

CN212410610U - Specific protein detection module and blood detection instrument

Info

Publication number: CN212410610U
Application number: CN201922129287.9U
Authority: CN
Inventors: 汤诚鹏; 许焕樟; 汪明月
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2019-11-30
Filing date: 2019-11-30
Publication date: 2021-01-26
Anticipated expiration: 2029-11-30

Abstract

The utility model provides a blood detection instrument and a specific protein detection module thereof, wherein the specific protein detection module comprises a liquid power device, a heating device and a plurality of detection liquid paths, and each detection liquid path comprises a mixing pool, a detection assembly, a first pipeline, a second pipeline, a third pipeline and a fourth pipeline; the liquid power device is used for carrying out reagent providing operation and sucking, spitting and mixing operation; the heating device is used for heating at least one of the blending pool, the detection component, the first pipeline, the second pipeline and the third pipeline; the first pipeline is connected with the mixing tank and used for discharging waste liquid; the second pipeline is connected with the blending pool and used for providing a hemolysis reagent to the blending pool through a liquid power device; the third pipeline is connected with the blending pool and used for providing an antibody reagent for the blending pool through a liquid power device; and the fourth pipeline connects the blending pool with the liquid power device and is used for carrying out suction, discharge and blending operation through the liquid power device.

Description

Specific protein detection module and blood detection instrument

Technical Field

The utility model relates to a medical treatment detection and analysis technical field, concretely relates to specific protein detection module, blood detecting instrument and detection method.

Background

At present, the clinical examination in hospitals generally needs to obtain the blood routine parameters and various specific protein parameters of patients, such as C-reactive protein (CRP), Serum Amyloid A (SAA), transferrin and the like, and the condition of the patients can be accurately judged after various detection results are integrated.

Doctors generally need to obtain conventional parameters of blood in a blood cell analyzer and specific protein parameters in a biochemical analyzer or a specific protein analyzer; doctors need to test on different instruments to obtain two results, and the operation is troublesome.

Moreover, most of the existing specific protein analyzers are single-channel detection, and the detection efficiency is low.

SUMMERY OF THE UTILITY MODEL

The utility model provides a specific protein detection module, blood detecting instrument and detection method to solve among the prior art current specific protein analyzer and mostly be the single channel detection, the slower technical problem of detection efficiency.

In order to solve the technical problem, the utility model discloses a technical scheme be: the specific protein detection module is characterized by comprising a liquid power device, a heating device and a plurality of detection liquid paths, wherein each detection liquid path comprises a mixing pool, a detection assembly, a first pipeline, a second pipeline, a third pipeline and a fourth pipeline;

the liquid power device is used for carrying out reagent providing operation and sucking, spitting and uniformly mixing operation;

the heating device is used for heating at least one of the blending pool, the detection assembly, the second pipeline, the third pipeline and the fourth pipeline;

the first pipeline is connected with the blending pool and is used for discharging waste liquid;

the second pipeline is connected with the blending pool and is used for providing a hemolysis reagent to the blending pool through the liquid power device;

the third pipeline is connected with the blending pool and is used for providing an antibody reagent for the blending pool through the liquid power device;

the fourth pipeline will the mixing pond with the fluid power device is connected for through the fluid power device inhales and tells the mixing operation detection assembly is used for right the reagent behind the mixing pond detects.

The utility model has the advantages that: be different from prior art's condition, the utility model provides a specific protein detection module makes up the conventional measuring module of blood after, can adopt a sample, detects the parameter of the conventional and multiple specific protein project of blood simultaneously in same blood detecting instrument, revises specific protein parameter through the HCT parameter, realizes the whole blood test, and convenient operation improves the holistic detection efficiency of hospital.

The utility model provides a blood detecting instrument can make up specific protein project at will, makes things convenient for the project extension of specific protein, can realize that the multichannel detects same specific protein project simultaneously for whole detection speed.

The utility model provides a specific protein detection module can save the cost with the mixing syringe of multichannel and the sharing of washing pipeline, convenient overall arrangement.

The utility model provides a specific protein detection module has increased mixing pipeline heat preservation device, is favorable to the mixing in-process to keep warm to reaction liquid, is favorable to guaranteeing the result accuracy under different ambient temperature.

The utility model provides a specific protein detection module has set up solitary washing liquid, and is good to the determine module cleaning performance, still can guarantee the accuracy of sample result behind a plurality of samples of continuous test.

The utility model provides a specific protein detection module increases the diluent heating bath, has reduced the temperature influence, keeps apart diluent heating bath and mixing pipeline simultaneously, prevents to stew the bubble of back heating production and influences the mixing precision, is favorable to improving overall stability.

Drawings

Fig. 1 is a schematic view of a partial planar structure inside an analyzer according to an embodiment of the present invention;

FIG. 2 is a schematic perspective view of a detection device of the analyzer shown in FIG. 1;

fig. 3 is a schematic view of a partial three-dimensional structure inside an analyzer according to an embodiment of the present invention;

FIG. 4 is a schematic perspective view of a heating assembly of the detection apparatus shown in FIG. 2;

FIG. 5 is an exploded view of the heating assembly shown in FIG. 4;

FIG. 6 is a schematic perspective view of a heating block of the heating assembly shown in FIG. 5;

FIG. 7 is a schematic perspective view of a detection assembly of the detection apparatus shown in FIG. 2;

FIG. 8 is a schematic diagram of an exploded view of the detection assembly shown in FIG. 7;

FIG. 9A is a schematic perspective view of an optical detection cell of the detection apparatus shown in FIG. 2;

FIG. 9B is a schematic diagram of an exploded structure of the optical detection cell shown in FIG. 9A;

FIG. 10A is a schematic side view of another embodiment of an optical detection cell;

FIG. 10B is a schematic cross-sectional view of the optical detection cell shown in FIG. 10A;

FIG. 10C is a schematic diagram of a side view of another embodiment of an optical detection cell;

FIG. 10D is a schematic view of the optical detection cell shown in FIG. 10C on the other side;

FIG. 11 is a schematic perspective view of a mounting cup of the analyzer shown in FIG. 1;

FIG. 12 is an exploded view of the mounting cup shown in FIG. 11;

FIG. 13 is a side view of the mounting socket shown in FIG. 11;

FIG. 14 is a schematic perspective view of the mixing tank shown in FIG. 12;

FIG. 15 is a schematic cross-sectional view of the mixing tank shown in FIG. 14;

FIG. 16 is an exploded schematic view of the pre-heat tank shown in FIG. 12;

fig. 17 is a schematic perspective view of a bobbin according to an embodiment of the present invention;

fig. 18 is a side view of the bobbin cartridge shown in fig. 17;

fig. 19 is a schematic perspective view of a pipeline heating assembly according to an embodiment of the present invention;

FIG. 20 is a cross-sectional structural schematic view of the pipe heating assembly shown in FIG. 19;

FIG. 21 is an exploded view of the pipe heating assembly shown in FIG. 19;

fig. 22-26 are schematic structural views of a detection assembly provided in an embodiment of the present invention;

fig. 27-30 are simplified structural schematic diagrams of a detection apparatus according to an embodiment of the present invention;

FIG. 30A is a graph showing a comparison of reaction temperatures with and without diluent line heating;

fig. 31-33 are schematic diagrams of the liquid path of a specific protein detection module provided in the embodiments of the present invention.

Detailed Description

The technical solutions between the following embodiments can be combined with each other.

In a first embodiment, this embodiment provides a detection apparatus, as shown in fig. 1 to 6, the detection apparatus includes a stand 100, a heating component, a detection component, and an optical detection cell 120.

As shown in fig. 2, the vertical frame 100 may be a metal support or a plastic support, and is fixedly assembled in the housing of the analyzer, the heating assembly may be obliquely fixed on the vertical frame 100, the heating assembly includes a heating block 110, the heating block 110 may be made of aluminum material or copper material, the heating block 110 is provided with a plurality of assembling cavities 113 for receiving the optical detection cell 120, the volume of the assembling cavities 113 matches with the volume of the optical detection cell 120, the assembling cavities 113 fully surround the optical detection cell 120, only the bottom end surface of the optical detection cell 120 is exposed, the contact area between the heating block 110 and the optical detection cell 120 is large, the heat conduction effect is good, and the liquid to be detected in the optical detection cell 120 can be well temperature controlled. The detection component is fixed with the heating block 110, the detection component comprises a base body 130, the base body 130 is provided with a notch 134 matched with the heating block 110 in a clamping mode, and the optical detection cell 120 is arranged in the notch 134 and is loaded into the assembly cavity 113 through the bearing of the base body 130.

As shown in fig. 2, 7, and 8, the heating block 110 further has a light through hole 114 penetrating through the assembling cavity 113, the substrate 130 further has a light incident channel 133, a first light emitting channel 131, and a second light emitting channel 132, the gap 134 is disposed between the light incident channel 133 and the first light emitting channel 131 and the second light emitting channel 132, the light incident channel 133 is aligned with the first light emitting channel 131, an axis of the second light emitting channel 132 is inclined with respect to an axis of the first light emitting channel 131, and the light through hole 114 is aligned with the light incident channel 133 when the detecting assembly is fixed to the heating block 110.

In order to fix the detecting element and the heating block 110, the base 130 has an assembling plate 136 integrally extending from one side of the notch 134 close to the light inlet channel 133, the assembling plate 136 has a first assembling hole 137, a first connecting hole 111 is formed in a side wall of the heating block 110 corresponding to the first assembling hole 137, the detecting element can be locked with respect to the heating block 110 by passing a screw through the first assembling hole 137 and screwing into the first connecting hole 111, and the first connecting hole 111 is located at a side edge of the light through hole 114.

Further, the base 130 is further provided with a second assembling hole 138 penetrating to the bottom of the notch 134, a second connecting hole 112 is formed at the bottom end of the heating block 110 corresponding to the second assembling hole 138, the detection assembly can be locked with respect to the heating block 110 by passing a screw through the second assembling hole 138 and screwing the screw into the second connecting hole 112, and the second connecting hole 112 is located at the side of the assembling cavity 113.

The embodiment of the utility model provides an in, assembly chamber 113 is a plurality of that parallel interval set up, for example 6 shown in fig. 2, and detection subassembly, optical detection pond 120 are a plurality ofly, and it is provided with multiunit guide rib 115 so that breach portion 134 and assembly plate 136 of base member 130 and heating block 110 counterpoint centre gripping cooperation to go back parallel interval on the lateral wall of heating block 110, and guide rib 115's extending direction is parallel with assembly chamber 113's extending direction.

As shown in fig. 2 and fig. 4 to fig. 10D, the detection device further includes a waterproof sealing ring 101, the optical detection cell 120 includes a cell body 121 and a liquid injection tube 125, a through hole 116 is formed at the top end of the heating block 110 and is communicated with the assembly cavity 113 to allow the liquid injection tube 125 to extend out, the waterproof sealing ring 101 is located in the assembly cavity 113, is sleeved on the periphery of the liquid injection tube 125, and is sandwiched between the optical detection cell 120 and the heating block 110, so that external liquid can be prevented from entering the optical detection cell 120 to affect the detection result.

As shown in fig. 1 and 2, the heating block 110 is obliquely fixed on the vertical frame 100 so that the assembly chamber 113 is obliquely arranged, and the optical detection cell 120 and the detection assembly are obliquely arranged relative to the horizontal plane.

The detection device further comprises a pipeline heating assembly 300, the pipeline heating assembly 300 is used for winding a pipeline and heating the pipeline, the vertical frame 100 comprises a first support arm 102 and a second support arm 104, the heating block 110 is fixed through the first support arm 102, the pipeline heating assembly 300 is arranged on the side edge of the heating block 110 through the second support arm 104, and the pipeline heating assembly 300 is lower than the liquid injection pipe 125, so that the pipeline wound on the pipeline heating assembly 300 can be bent and butted with the liquid injection pipe 125 with smaller curvature when being led out, the bubble discharge can be facilitated, the bubble problem can be reduced, if the pipeline heating assembly 300 is higher than the liquid injection pipe 125, the pipeline wound on the pipeline heating assembly 300 is bent with larger curvature and butted with the liquid injection pipe 125 when being led out, the bubbles are not easy to discharge, and the relatively serious bubble problem can be generated.

As shown in fig. 4 to 6, the heating assembly further includes a heating film 117, a temperature sensor 118 and an over-temperature protection switch 119, the heating block 110 may be made of a material with good thermal conductivity, such as aluminum or copper, the heating film 117 is attached to the surface of the heating block 110, and the temperature sensor 118 and the over-temperature protection switch 119 are embedded in the heating block 110. The two ends of the heating block 110 can be provided with mounting holes 103 to be fixed with the first support arm 102 of the stand 100, the surface of the heating block 110 for attaching the heating film 117 can be provided with a protrusion 106, the protrusion 106 enables the cross section of the heating block 110 to be L-shaped, and the over-temperature protection switch 119 can be embedded in the protrusion 106, so that the structure of the heating block 110 can be compact.

The detection device provided by the embodiment has a novel structure, the optical detection cell 120 is arranged in the notch part 134 and is loaded into the assembly cavity through the support of the base body 130, the assembly and disassembly are convenient, the maintenance is facilitated, the influence of water leakage on the detection device can be reduced, and the problem of bubbles can be reduced. The embodiment also provides a sample analyzer, which comprises the detection device.

In a second embodiment, this embodiment provides a detection assembly, as shown in fig. 7 to 8, the detection assembly includes a base 130, a light receiver, and a fixing assembly. In one embodiment, the securing assembly includes a securing member 139 and a sealing ring 140.

The base 130 may be made of plastic or metal, the fixing element 139 is fixed to the base 130 to assemble the light receiver in the base 130, and the sealing ring 140 is sandwiched between the fixing element 139 and the light receiver.

Specifically, as shown in fig. 8, the base 130 is provided with a first light-emitting channel 131 and a second light-emitting channel 132, an axis of the second light-emitting channel 132 is inclined with respect to an axis of the first light-emitting channel 131, side edges of the first light-emitting channel 131 and the second light-emitting channel 132 are provided with a first fixing hole 143, and the fixing member 139 is a fixing plate fixed to the base 130 by a screw.

The light receiver comprises a first photodiode 141 and a second photodiode 142, the first photodiode 141 is assembled in the first light-emitting channel 131, the second photodiode 142 is assembled in the second light-emitting channel 132, the detection assembly further comprises a transmission light filtering block 144, a lens fixing block 145 and a lens 146, the transmission light filtering block 144 is arranged in the first light-emitting channel 131 and located on the light-in side of the first photodiode 141, the lens fixing block 145 and the lens 146 are arranged in the second light-emitting channel 132, and the lens 146 is fixed by the lens fixing block 145 and located on the light-in side of the second photodiode 142.

The base 130 is further provided with a notch 134, the notch 134 is communicated with the first light-emitting channel 131 and the second light-emitting channel 132, the notch 134 is used for accommodating the optical detection cell 120, one side of the notch 134, which is close to the first light-emitting channel 131 and the second light-emitting channel 132, is provided with an arc-shaped groove 135, the side of the arc-shaped groove 135 is provided with a second fixing hole 147, the second fixing hole 147 is used for fixing a light barrier (not shown), and the light barrier is used for blocking light at two ends of the arc-shaped groove 135.

The detecting assembly further includes a first light barrier 148, the first light barrier 148 is fixed to the substrate 130 and extends into the gap 134 near one side of the first light-emitting channel 131 and the second light-emitting channel 132, the first light barrier 148 is provided with a light-passing groove 149, and the light-passing groove 149 is opposite to the first light-emitting channel 131 and the second light-emitting channel 132.

Base member 130 has assembly plate 136 in the integrative extension of one side that first light-emitting channel 131 and second light-emitting channel 132 were kept away from to breach portion 134, is equipped with first pilot hole 137 on assembly plate 136, and first pilot hole 137 is used for supporting base member 130 fixed mounting, and it is easy to know to combine fig. 2, and assembly plate 136 can with the spout sliding fit that two guide ribs 115 constitute, passes first pilot hole 137 and screw in first connecting hole 111 through the screw and can lock the relatively heating piece 110 of detecting component.

As shown in fig. 8, the detecting assembly further includes a laser 156 and a second diaphragm 150, the substrate 130 is further provided with a light inlet channel 133, the light inlet channel 133 is communicated with the notch 134 and aligned with the first light outlet channel 131, and the laser 156 and the second diaphragm 150 are installed in the light inlet channel 133. The number of the second light barriers 150 may be one, two, or three, and the first light barrier 148 and the second light barrier 150 function such that the light-transmitting portions function as light-transmitting portions and the light-blocking portions function as light-blocking portions.

As shown in fig. 2, 7 and 8, the base 130 is further provided with a first slot 151 and a second slot 152, the first slot 151 is disposed near the outer end of the light-entering channel 133 so that one end of the base 130 constitutes a clamping block 153, the second slot 152 is disposed on the clamping block 153 and communicates with the light-entering channel 133, both sides of the clamping block 153 are provided with through connection holes 154, and the connection holes 154 are used for setting screws so that the clamping block 153 clamps or releases the laser 156.

As shown in fig. 7 and 8, the fixing plate is provided with a slot 155 to allow lead wires of the first photodiode 141 and the second photodiode 142 to be drawn out.

The detection assembly provided by the embodiment can improve the assembly precision by arranging the sealing ring 140 between the fixing member 139 and the light receiver, so that the receiving plane of the light receiver is relatively flat.

In another embodiment, the fixing component is a small circuit board, the optical receiver is soldered on one surface of the circuit board, the circuit board is fixed with the base 130 by a screw or an adhesive, the other surface of the circuit board is provided with a terminal, and a signal of the optical receiver can be led out through the terminal and is connected to the main control circuit board of the analyzer for processing through a wire. The present embodiment also provides a sample analyzer, which includes the aforementioned detection assembly.

Third embodiment, as shown in fig. 9A and 9B, the present embodiment provides an optical detection cell 120, where the optical detection cell 120 includes a cell body 121 and a light-transmitting plate 124.

The cell body 121 is provided with a straight through groove 122 penetrating two opposite surfaces of the cell body 121 and a liquid injection passage 123 communicating with the through groove 122, and the light-transmitting plate 124 is fixed with the cell body 121 and closes the outer end of the through groove 122. The optical detection tank 120 is designed in a split mode, so that the problem that the difficulty is high in glass integrated design and manufacturing can be solved, specifically, the through groove 122 of the tank body 121 can be formed directly in a numerical control drilling or numerical control groove milling mode, and the liquid injection channel 123 can be formed in a numerical control drilling mode.

Specifically, the tank body 121 may be a metal tank body, a ceramic tank body, a glass tank body or a plastic tank body. The transparent plate 124 may be a glass plate or a plastic plate. The light-transmitting plate 124 and the cell body 121 can be fixed by adhesive bonding, laser welding or screws.

Cell body 121 may be provided with a raised post, a rib 126, a surrounding edge or a recessed area to position and assemble light-transmitting plate 124, and rib 126 may be a straight strip or an L-shape as shown in fig. 9B.

In an embodiment, the optical detection cell 120 may have a rectangular shape, the optical detection cell 120 has a pair of first side surfaces, a pair of second side surfaces and a pair of third side surfaces, the area of the first side surfaces is gradually reduced, the through slot 122 penetrates through the pair of first side surfaces, and the outer opening end of the liquid injection channel 123 is located on one of the third side surfaces.

Cell body 121 is equipped with two notes liquid passageway 123, and two notes liquid passageway 123 are vertical setting and are close to the relative end angle setting of third surface respectively in order to reduce the product volume. The two liquid injection channels 123 are respectively connected with a liquid injection pipe 125.

The cross section of the through groove 122 may be circular, oval, racetrack-shaped, rectangular, or regular polygon, and the racetrack-shaped is a shape surrounded by two parallel sides and two arc sides.

The optical detection cell 120 provided by the embodiment is assembled after being manufactured by adopting split type machining, so that the machining difficulty is greatly simplified, the machining efficiency is improved, the production cost is reduced, and the problem of high difficulty in design and manufacture by adopting a glass integrated mode can be avoided.

As shown in fig. 10A and 10D, the present embodiment further provides an optical detection cell, which includes a cell body 121, a light-transmitting plate (not shown, refer to the light-transmitting plate 124) and a liquid injection tube 125.

The cell body 121 is provided with a straight through groove 122 penetrating two opposite surfaces of the cell body 121, a liquid injection channel (not shown, refer to the liquid injection channel 123) communicating with the through groove 122, an open end of the liquid injection channel being located on an adjacent surface between the two opposite surfaces, and a liquid guide groove 127. The through groove 122 of the cell body 121 can be directly formed in a numerical control drilling or numerical control groove milling mode, the liquid injection channel 123 can be formed in a numerical control drilling mode, the liquid guide groove 127 can be formed in a numerical control groove milling mode, and the cell body is convenient to machine, regular in shape and high in precision.

The tank body 121 may be a metal tank body 121, a ceramic tank body 121, a glass tank body 121 or a plastic tank body 121. Cell body 121 may be rectangular in shape, or may be stepped as shown in fig. 10B and 10C, i.e., including a relatively thin lower portion and a relatively thick upper portion. The cross section of the through-groove 122 may be in a racetrack shape as shown in fig. 10A, the areas of both ends of the through-groove 122 may be the same or different, the bottom side of the through-groove 122 in the longitudinal section of the through-groove 122 is disposed horizontally or obliquely as shown in fig. 10B, in other embodiments, the cross section of the through-groove 122 may be in a circular hole shape (as shown in fig. 10D) or a square hole shape, when the areas of both ends of the through-groove 122 are different, for example, when the longitudinal section of the through-groove 122 shown in fig. 10A and 10B is in a right trapezoid shape, the internal volume of the optical detection cell may be reduced to prevent bubbles from being generated, the angle of the oblique side of the through-groove 122 may be the same as the angle of the scattered light, the scattered light may be emitted through the second light-emitting channel 132, the angle may effectively block the stray light, and the range of the included angle of the bottom side of the, 23 degrees, 23.5 degrees, 24 degrees, 26 degrees, 28 degrees, etc. The through groove 122 may be disposed at a thinner lower portion of the cell body 121, and an area of the corresponding light transmitting plate may be reduced to form flush assembly with the cell body 121 in a stepped shape.

The liquid guide groove 127 connects the through groove 122 with the liquid injection passage, the liquid guide groove 127 is connected with the liquid injection passage in an inclined manner, the liquid guide groove 127 is connected with the top end of the through groove 122 so as to facilitate the discharge of bubbles in the tank body 121, and the angle range of an acute angle formed by the liquid guide groove 127 relative to the vertical direction is less than or equal to 30 degrees, such as 23 degrees, 24 degrees, 25 degrees, 26 degrees, 27 degrees and the like. Liquid guide groove 127 sinks on two opposite surfaces of cell body 121, one end of liquid guide groove 127 is communicated with the end of through groove 122, the other end of liquid guide groove 127 is communicated with liquid injection channel, liquid injection channel is connected with liquid injection pipe 125, liquid injection pipe 125 and through groove 122 can be arranged in a staggered manner, so that liquid guide groove 127 communicated with liquid injection channel through groove 122 can be arranged in an inclined manner, the obliquely arranged liquid guide groove can enable bubbles to be difficult to accumulate in cell body 121, and liquid injection channel and liquid injection pipe 125 are two in number and arranged diagonally so as to enable the structure of the product to be compact.

The light-transmitting plate is fixed with the cell body 121 and closes the outer end of the through groove 122. The light-transmitting plate is a glass plate or a plastic plate. The light-transmitting plate and the cell body 121 are fixed by adhesive bonding, laser welding or screws. The cell body 121 may be provided with a convex pillar, a rib, a surrounding edge or a concave area for positioning and assembling the light transmission plate.

The optical detection pool 120 of the embodiment adopts a split design, so that the problem of high difficulty in design and manufacture of a glass integrated type can be avoided, the accurate control of the volume and the shape of the inner cavity of the optical detection pool 120 is facilitated, the requirements of the shape rule and the small volume of the inner cavity and the requirements of the local inclined plane of the inner cavity are easily met, the generation difficulty and the cost are reduced, the bubble influence is reduced by the optimized structural design, the detection condition is optimized, and the detection precision is improved.

This embodiment still provides a sample analyzer, and this sample analyzer includes aforementioned optical detection pond 120, mixing pond and determine module, and mixing pond and optical detection pond intercommunication, determine module are equipped with the breach portion of holding optical detection pond, and determine module still includes laser instrument and the photoreceiver of locating breach portion both sides respectively. The mixing pool and the detection component refer to other embodiments.

A fourth embodiment, this embodiment provides an assembly seat, as shown in fig. 11 to 16, the assembly seat includes a first assembly body 210, two surfaces of the first assembly body 210 facing away from each other are respectively provided with a first assembly groove 211 and a second assembly groove 212, the first assembly groove 211 and the second assembly groove 212 are respectively used for arranging a blending pool 240 and a preheating pool 250, the number of the first assembly groove 211 and the second assembly groove 212 may be single or laterally expanded into a plurality (for example, 6 as shown in fig. 12), and the position of the first assembly groove 211 is higher than that of the second assembly groove 212 so that the blending pool 240 is arranged higher than the preheating pool 250. The liquid in the preheating tank 250 needs to be input into the blending tank 240 for reaction, and when bubbles are generated by heating the liquid in the preheating tank 250, the bubbles in the preheating tank 250 are easily discharged out of the preheating tank 250 along with the output of the liquid under the condition that the blending tank 250 is higher than the preheating tank 250 due to the lower density of the bubbles.

As shown in fig. 13, the first assembly body 210 is stepped and includes a connection portion 214, a first fitting portion 216 extending upward from one side of the connection portion 214, and a second fitting portion 218 extending downward from the other side of the connection portion 214, the first fitting groove 211 is recessed in the first fitting portion 216 and the connection portion 214, and the second fitting groove 212 is recessed in the second fitting groove 212 and the connection portion 214.

As shown in fig. 12, the assembly base further includes a second assembly body 220 and a third assembly body 230, the second assembly body 220 is provided with a third assembly groove 221 and is fastened with the first assembly groove 211 to set up a blending tank 240, and the third assembly body 230 is provided with a fourth assembly groove 231 and is fastened with the second assembly groove 212 to set up a preheating tank 250. The first assembly 210, the second assembly 220, and the third assembly 230 are made of aluminum material or copper material with good thermal conductivity.

Of course, in other embodiments, the mounting seat may be a single component, and the first mounting groove 211 and the second mounting groove 212 are cavities disposed inside the first assembly 210 and matched with the blending pool 240 and the preheating pool 250, so as to reduce material cost.

As shown in fig. 12, this embodiment further provides a heating assembly, which includes a blending pool 240, a preheating pool 250, a first heating body 261, a second heating body 262 and the aforementioned assembly seat, where the blending pool 240 and the preheating pool 250 are made of corrosion-resistant materials, the blending pool 240, the preheating pool 250 and the assembly seat are filled with heat-conducting materials, and the first heating body 261 and the second heating body 262 are respectively used for heating the blending pool 240 and the preheating pool 250.

As shown in fig. 14 and 15, a first liquid outlet pipe 241 and a waste discharge pipe 242 are disposed at the bottom of the mixing tank 240, a first liquid inlet pipe 243 is disposed at a position of the mixing tank 240 close to the top, and a first reinforcing block 244 and a second reinforcing block 245 are further disposed at the first liquid outlet pipe 241 and the first liquid inlet pipe 243 of the mixing tank 240, respectively. When the blending tank 240 is not used, the blending tank needs to be soaked with liquid, and if the position of the first liquid inlet pipe 243 is low, the liquid in the blending tank 240 is easy to contact with the reagent in the first liquid inlet pipe 243 to pollute the reagent, so the installation position of the first liquid inlet pipe 243 is higher than the height of the soaking liquid.

The first liquid inlet pipe 243 is obliquely arranged relative to the blending pool 240, and the included angle of the first liquid inlet pipe 243 relative to the axis of the blending pool 240 can be 30 +/-15 degrees.

The bottom of the preheating tank 250 is provided with a second liquid inlet pipe 251, the top of the preheating tank 250 is provided with a second liquid outlet pipe 252, and the position of the second liquid outlet pipe 252 is lower than that of the first liquid inlet pipe 243. The top of the preheating tank 250 is also provided with a plug body 253.

The heating element still includes excess temperature protection switch 263, temperature sensor 264, first installing support, second installing support and top cap 276, excess temperature protection switch 263, temperature sensor 264 is installed in first assembly body 210, first installing support is including the first connecting plate 271 that connects gradually, second connecting plate 272 and third connecting plate 273, first connecting plate 271 is connected with first assembly body 210 and is parallel with third connecting plate 273, the second installing support includes fourth connecting plate 274 and fifth connecting plate 275 that the cross-section is L connection, fourth connecting plate 274 with be used for the bearing and connect the bottom of first assembly body 210, fifth connecting plate 275 and third connecting plate 273 are located the coplanar, conveniently carry out fixed mounting, be equipped with on the top cap 276 with fixed orifices 213, the fixed orifices 278 that 232 corresponds and the perforation 277 that corresponds with the second drain pipe 252 that preheats the pond.

The heating element that this embodiment provided compact structure, convenient assembling, heat conduction efficiency is better, the first feed liquor pipe 243 of mixing pond 240 sets up the position relatively higher and be the slope setting, reagent is difficult for receiving the pollution, the reagent of entering is difficult for spattering on the pool wall of mixing pond 240, through first heating member 261, second heating member 262 is respectively to mixing pond 240, preheat pond 250 and heat, can control different heating power, reach different heating effects, and then improve the accuracy that detects. The present embodiments also provide a sample analyzer that includes the aforementioned heating assembly.

In a fifth embodiment, the present embodiment provides a pipeline heating assembly 300, as shown in fig. 17 to 21, the pipeline heating assembly 300 includes a heating cylinder 301, a pipe penetrating channel 302 penetrating through an arc-shaped wall of the heating cylinder 301 is provided on an outer circumferential surface of the heating cylinder 301, the outer circumferential surface of the heating cylinder 301 is used for winding a pipeline, and the pipe penetrating channel 302 is used for allowing the pipeline to pass through and forming an anti-drop limit for the pipeline.

The tube-passing channel 302 may be a straight strip or a curved strip. The tube passing channel 302 is away from the diameter of the heating cartridge 301 and close to the outer circumferential surface of the heating cartridge 301. If the tube-through channel 302 is directly opened on the diameter of the heating cylinder 301, the tube-through channel may be bent at a larger angle, which is not favorable for the transmission of the internal reagent.

The opening size of poling passageway 302 one end is greater than the opening size of the other end so that carry out the poling operation, penetrates convenient operation from the great one end of opening to it is better to wear out spacing effect from the less one end of opening.

The heating cylinder 301 is provided with a plurality of flanges 303, for example, three flanges are shown in fig. 17, the pipe penetrating channels 302 are arranged close to the flanges 303, two pipe penetrating channels 302 are arranged between two adjacent flanges to wind a coil of pipeline, two leading-out ends of the pipeline penetrate through the two pipe penetrating channels 302 respectively, and the pipeline can be well prevented from loosening.

As shown in fig. 21, the pipe heating assembly 300 further includes a fixing clip 310, the fixing clip 310 is disposed on the outer periphery of the heating cylinder 301 and is provided with an opening 312 corresponding to the pipe passing passage 302 to allow the pipes (304, 305) to be led out. The stationary clamping plate 310 may be two, and each stationary clamping plate 310 may include an arc-shaped portion and a flat plate portion, and the flat plate portion may be provided with a locking hole for locking a screw. The pipeline heating assembly 300 further comprises heat insulation cotton 330, the heat insulation cotton 330 is sleeved on the periphery of the fixed clamping plate 310 and provided with a cut part 332 corresponding to the opening 312 to allow the pipelines (304, 305) to be led out, one end of the heat insulation cotton 330 is further provided with extension cotton 334, the extension cotton 334 at least partially covers the end part of the heating cylinder 301 to improve the heat insulation effect, and meanwhile, the extension cotton 334 can play a role in limiting assembly, so that the heat insulation cotton 330 and the fixed clamping plate 310 are not easy to relatively displace.

The pipe heating assembly 300 further includes a temperature protection switch 342, a temperature sensor 344, and a heating rod 346, and an end of the heating cartridge 301 is provided with a fitting groove 341, and the fitting groove 341 is used to fit the temperature protection switch 342, the temperature sensor 344, and the heating rod 346. The pipe heating assembly 300 further includes a mounting plate 350, and the mounting plate 350 is fixed to one end of the heating cartridge 301 for fixedly assembling the heating cartridge 301.

The pipeline heating assembly 300 that this embodiment provided can carry out accurate control by temperature change on reagent delivery path, has avoided reagent heat loss in transportation process, has improved heating efficiency and detection precision. The present embodiment also provides a sample analyzer comprising the aforementioned tube heating assembly 300.

In a sixth embodiment, this embodiment provides a detection apparatus, as shown in fig. 1 to 12, the detection apparatus includes a blending tank 240, a preheating tank 250, a diluent tank 270, an optical detection tank 120, a first heating body 261, a second heating body 262, a third heating body 263, and a heating component.

Wherein, the first heating body 261 is attached to the outer side of the blending pool 240 and used for heating the blending pool 240, the preheating pool 250 is arranged at the side edge of the blending pool 240 and communicated with the blending pool 240 through a pipeline, the second heating body 262 is attached to the outer side of the preheating pool 250 and used for heating the preheating pool 250, the diluent pool 270 is arranged at the side edge of the blending pool 240 and communicated with the blending pool 240 through a pipeline, the third heating body 263 is attached to the outer side of the diluent pool 270 and used for heating the diluent pool 270,

the optical detection cell 120 is arranged on the side of the blending cell 240 and is communicated with the blending cell 240 through a pipeline, the heating component comprises a heating block 110, and the heating block 110 coats the outer side of the optical detection cell 120 and is used for heating the optical detection cell 120.

As shown in fig. 17 to 21, the detection assembly further includes a pipeline heating assembly 300, and the pipeline heating assembly 300 includes a heating cylinder 301, and an outer circumferential surface of the heating cylinder 301 is used for winding the pipeline to heat the pipeline. The outer peripheral surface of the heating cylinder 301 is provided with a pipe penetrating channel 302 penetrating through the arc-shaped wall of the heating cylinder 301, and the pipe penetrating channel 302 is used for allowing a pipeline to penetrate and forming anti-falling limit for the pipeline. The heating cylinder 301 is provided with a plurality of flanges 303, and the pipe penetrating channel 302 is arranged close to the flanges 303.

As shown in fig. 11 to 16, the detection assembly further includes an assembly seat, the preheating tank 250 and the blending tank 240 are assembled through the assembly seat, the assembly seat includes a first assembly body 210, two surfaces of the first assembly body 210 facing away from each other are respectively provided with a first assembly groove 211 and a second assembly groove 212, the first assembly groove 211 and the second assembly groove 212 are respectively used for setting the blending tank 240 and the preheating tank 250, and the position of the first assembly groove 211 is higher than that of the second assembly groove 212 so that the blending tank 240 is higher than that of the preheating tank 250.

The first assembly body 210 is assembled in a ladder manner and includes a connecting portion 214, a first assembling portion 216 extending upward from one side of the connecting portion 214, and a second assembling portion 218 extending downward from the other side of the connecting portion 214, the first assembling groove 211 is recessed in the first assembling portion 216 and the connecting portion 214, and the second assembling groove 212 is recessed in the second assembling groove 212 and the connecting portion 214.

The assembly base further comprises a second assembly body 220 and a third assembly body 230, the second assembly body 220 is provided with a third assembly groove 221 and is buckled with the first assembly groove 211 to form a blending pool 240, and the third assembly body 230 is provided with a fourth assembly groove 231 and is buckled with the second assembly groove 212 to form a preheating pool 250.

As shown in fig. 5, the heating assembly includes a heating block 110 and a heating film 117 attached to a side of the heating block 110, and the heating block 110 is provided with a mounting cavity 113 for receiving the optical detection cell 120.

As shown in fig. 2 and 8, the detecting device further includes a stand 100 and a detecting component.

Wherein, the stand 100 comprises a first support arm 102 and a second support arm 104, the heating component is fixed by the first support arm 102, the pipeline heating component 300 is arranged at the side of the heating block 110 by the second support arm 104,

the detection assembly further includes a substrate 130, a laser 156, and a light receiver.

The substrate 130 is fixed to the heating block 110, the substrate 130 is provided with a notch 134 clamped and matched with the heating block 110, the light inlet channel 133, the first light outlet channel 131 and the second light outlet channel 132, the notch 134 is arranged between the light inlet channel 133 and the first light outlet channel 131 and the second light outlet channel 132, the light inlet channel 133 is aligned to the first light outlet channel 131, the axis of the second light outlet channel 132 is inclined relative to the axis of the first light outlet channel 131, the laser 156 is arranged in the light inlet channel 133, the light receiver comprises a first photodiode 141 and a second photodiode 142, the first photodiode 141 is assembled in the first light outlet channel 131, and the second photodiode 142 is assembled in the second light outlet channel 132.

The detection device that this embodiment provided heats respectively a plurality of ponds through a plurality of heating members, and the temperature of a plurality of heating members can set up the difference in order to provide the temperature demand that corresponds, can realize the rapid heating, and accurate heat preservation improves and detects the precision. The embodiment also provides a sample analyzer, which comprises the detection device.

The embodiment also provides a detection device, which comprises an optical detection cell, a first heater and a second heater, wherein the first heater is used for heating the optical detection cell; the second heater is used for heating the reagent input into the optical detection cell; wherein, the heating temperature of first heater and second heater is the differentiation setting, for example the heating temperature of second heater is higher than the heating temperature of first heater 1-7 degrees, and of course, in other test operating modes, the heating temperature of second heater also can be less than the heating temperature of first heater, and real reagent detects needs and decides. In one embodiment, the heating temperature of the optical detection cell can be set to 34-36 degrees, the heating temperature of the preheating cell and the mixing cell can be set to 40-42 degrees, the heating temperature of the diluent cell can be set to 36-38 degrees, and the heating temperature of the pipeline heating assembly can be set to 34-36 degrees.

The utility model provides a detection device heats respectively and the temperature of two at least heating members is the differentiation setting through two at least heaters to the reagent of optical detection pond and input optical detection pond, can realize that reagent rapid heating, and the optical detection pond is accurate keeps warm, has improved detection efficiency and detection precision.

The second heater includes an antibody reagent heater for heating the antibody reagent input into the optical detection cell, and since the antibody reagent is usually dispensed from a refrigerated state, heating the antibody reagent by the second heater with a relatively high heating temperature can shorten the heating time and improve the heating efficiency, wherein the antibody reagent can be heated in a pipeline (for example, by using the pipeline heating assembly 300 described below) or in the cell body (for example, the blending cell 240 described above). The second heater may comprise a hemolysis reagent heater for heating the hemolysis reagent input to the optical detection cell, and the hemolysis reagent may be heated in a tube (e.g., using the tube heating assembly 300 described below) or in a cell body (e.g., the mixing cell 240 described above). The second heater may also include a diluent heater for heating the diluent input to the optical detection cell, the diluent may be heated in a conduit (e.g., using conduit heating assembly 300 described below) or in the cell body, and the diluent heater may refer to third heater 263 in fig. 3.

The detection device further comprises a pipeline and a pool body which are directly or indirectly communicated with the optical detection pool, the pipeline is bent naturally or coiled, and the pipeline can be heated by the pipeline heating assembly 300 when coiled.

The cell body includes the mixing pond, and mixing pond and optical detection pond intercommunication, mixing pond are used for the input antibody reagent, and the second heater heats the mixing pond and then heats the antibody reagent. Or, the cell body includes the mixing pond, and mixing pond and optical detection pond are mixing and detect integrative pond, in other words, optical detection pond is used for receiving the reagent after the mixing or is used for receiving the reagent and carries out the reagent mixing in optical detection pond, and optical detection pond undertakes detection function and mixing function. The cell body still can include preheating the pond, preheats the pond and is used for inputing hemolysis reagent, preheats pond and mixing pond intercommunication, perhaps preheats pond and the integrative pond intercommunication of mixing detection.

The first heater and the second heater are heat-conducting substrates provided with heating rods or heating films, and the heat-conducting substrates are block-shaped, step-shaped (such as an assembly seat described in the fourth embodiment), cylindrical (such as a pipe heating assembly 300 described below), or box-shaped.

As shown in fig. 22, the present embodiment also includes a detection device, which includes a preheating tank 411, a blending tank 412, an optical detection tank 413, a blending pipe 414, a diluent pipe 415, a first heater 511, a second heater 512, a third heater 513, and a fourth heater 514.

Wherein, the mixing tank 412 is communicated with the preheating tank 411; the optical detection cell 413 is communicated with the blending cell 412; the preheating tank 411 and the blending tank 412 are arranged in a second heater 512, and the second heater 512 is used for heating the preheating tank 411 and the blending tank 412. The optical detection cell 413 is disposed in the first heater 511, and the first heater 511 is used for heating the optical detection cell 413, wherein the temperature of the second heater 512 can be 1-3 degrees higher than the temperature of the first heater 511 to achieve the effect of rapid heating. The blending pipeline 414 is communicated with the optical detection cell 413 and is arranged in the third heater 513, the third heater 513 is used for heating the blending pipeline 414, and the temperature of the third heater 513 can be 1-2 ℃ higher than that of the first heater 511, so that a good heat preservation effect is achieved. The diluent pipeline 415 is communicated with the blending pipeline 414, the fourth heater 514 is used for heating the diluent pipeline 415, and the temperature of the fourth heater 514 can be 1-3 ℃ higher than that of the first heater 511 so as to achieve the effect of rapid heating.

As shown in fig. 23, the present embodiment is also a detection apparatus including a preheating well 421, a kneading well 422, an optical detection well 423, a buffer well 424, a first heater 521, a second heater 522, and an antibody reagent storage 426.

The antibody reagent storage device 426 is communicated with the blending pool 422 through a pipeline and is used for inputting an antibody reagent into the blending pool 422, and the blending pool 422 is communicated with the preheating pool 421; the optical detection cell 423 is communicated with the blending cell 422; the preheating tank 421 and the blending tank 422 are arranged in the second heater 522, and the second heater 522 is used for heating the preheating tank 421 and the blending tank 422. The buffer cell 424 is communicated with the optical detection cell 423 and is jointly arranged in the first heater 521, and the temperature of the second heater 522 can be 1-3 degrees higher than that of the first heater 521 to achieve the effect of rapid heating.

As shown in fig. 24, the present embodiment also provides a detection apparatus including a preheating tank 431, a kneading detection integrated tank 430, a kneading line 434, a first heater 531, a second heater 532, and an antibody reagent tank 436.

Wherein the optical detection tank and the blending tank share the first tank body 430; the antibody reagent storage device 436 is communicated with the first tank body 430 through a pipeline and is used for inputting an antibody reagent into the first tank body 430, the preheating tank 431 is communicated with the first tank body 430 and is arranged in the first heater 531, and the first heater 531 is used for heating the preheating tank 431 and the first tank body 430. The blending pipeline 434 is communicated with the first tank body 430 and is arranged in the second heater 532, and the temperature of the second heater 532 can be 1-2 ℃ higher than that of the first heater 531 so as to achieve a better heat preservation effect.

As shown in fig. 25, the present embodiment is also a detection apparatus including a preheating bath 441, a kneading bath 442, an optical detection bath 443, a kneading line 444 (or buffer bath), a diluent line 445, a first heater 541, and a second heater 542.

Wherein, the blending tank 442 is communicated with the preheating tank 441; the optical detection cell 443 is communicated with the blending cell 442, and the blending pipeline 444 is communicated with the optical detection cell 443; the preheating tank 441, the blending tank 442, the optical detection tank 443, and the blending pipe 444 are disposed in the first heater 541, and the first heater 541 is used for heating the preheating tank 441, the blending tank 442, the optical detection tank 443, and the blending pipe 444. The diluent pipeline 445 is communicated with the blending pipeline 444, the second heater 542 is used for heating the diluent pipeline 445, and the temperature of the second heater 542 can be 1-3 degrees higher than that of the first heater 541, so that the effect of rapid heating is achieved.

As shown in fig. 26, the present embodiment is also a detection apparatus including a preheating chamber 451, a kneading chamber, an optical detection chamber, a kneading line 454, a first heater 551, and an antibody reagent chamber 456.

The optical detection pool and the blending pool share the first pool body 450, the antibody reagent pool 456 is communicated with the first pool body 450 through a pipeline and is used for inputting an antibody reagent into the first pool body 450, and the preheating pool 451, the first pool body 450 and the blending pipeline 454 (or a buffer pool) are jointly arranged in the first heater 551.

In the above embodiments, the first heater is a heat conductive substrate provided with a heating rod or a heating film, and conducts heat by direct contact or by air. The utility model also provides a sample analyzer, sample analyzer include preceding arbitrary embodiment detection device.

A seventh embodiment, the present embodiment provides a detection apparatus, as shown in fig. 1 to fig. 21, the detection apparatus includes a preheating tank 250, a blending tank 240, a diluent tank 270, an optical detection tank 120, and a pipeline heating assembly 300, the preheating tank 250, the blending tank 240, the diluent tank 270, and the optical detection tank 120 are connected through a pipeline, the pipeline heating assembly 300 includes a heating barrel 301, and an outer peripheral surface of the heating barrel 301 is used for winding a pipeline to heat the pipeline.

As shown in fig. 17 to 21, a pipe penetrating channel 302 penetrating through the arc-shaped wall of the heating cylinder 301 is provided on the outer circumferential surface of the heating cylinder 301, and the pipe penetrating channel 302 is used for allowing the pipe to pass through and forming an anti-drop limit for the pipe. The tube passing channel 302 is in a straight strip shape or a bent strip shape. The tube passing passage 302 is away from the diameter of the heating cartridge 301 and close to the outer circumferential surface of the heating cartridge 301. The heating cylinder 301 is provided with a plurality of flanges 303, and the pipe penetrating channel 302 is arranged close to the flanges 303.

The detection device further comprises an assembly seat, the preheating pool 250 and the blending pool 240 are assembled through the assembly seat, the assembly seat comprises a first assembly body 210, two surfaces, away from each other, of the first assembly body 210 are respectively provided with a first assembly groove 211 and a second assembly groove 212, the first assembly groove 211 and the second assembly groove 212 are respectively used for setting the blending pool 240 and the preheating pool 250, and the position of the first assembly groove 211 is higher than that of the second assembly groove 212 so that the blending pool 240 is higher than that of the preheating pool 250.

As shown in fig. 12, the first assembly body 210 is formed in a stepped manner, and includes a connection portion 214, a first assembly portion 216 extending upward from one side of the connection portion 214, and a second assembly portion 218 extending downward from the other side of the connection portion 214, the first assembly groove 211 is recessed in the first assembly portion 216 and the connection portion 214, and the second assembly groove 212 is recessed in the second assembly groove 212 and the connection portion 214.

The detection device comprises a first heating body 261, a second heating body 262 and a third heating body 263, wherein the first heating body 261 is attached to the outer surface of the second assembling seat and used for heating the blending pool 240, the second heating body 262 is attached to the outer surface of the third assembling seat and used for heating the preheating pool 250, and the third heating body 263 is attached to the outer surface of the diluent pool 270 and used for heating the diluent pool 270. The detection device that this embodiment provided can heat and keep warm the reagent in the pipeline through adding pipeline heating element 300, can improve and detect the precision, and this embodiment still provides a sample analyzer, and this sample analyzer includes aforementioned detection device.

In an eighth embodiment, this embodiment provides a detection apparatus, as shown in fig. 27 and 28, the detection apparatus includes a heating element 410, a container 420, a fluid dynamic device 430, and a reagent pipeline 440, the reagent pipeline 440 is connected between the container 420 and the fluid dynamic device 430, at least a portion of the reagent pipeline 440 is densely wound and disposed on the heating element 410, the reagent pipeline 440 is used for conveying a reagent to the container 420 through the fluid dynamic device 430, or the fluid dynamic device 430 is used for sucking and discharging a blending reagent, and the reagent is heated by the heating element 410 during the conveying or sucking, discharging and blending process of the fluid dynamic device 430. Of course, the detection device further comprises other components such as a sampling needle and a reversing valve, and the components are not described in detail in the application because the components do not relate to the utility model.

The hydrokinetic device 430 may be a syringe, a quantitative pump or an air pump in the analyzer, and the heating element 410 and the container 420 may be the same device or different devices.

Specifically, when the heating element 410 and the container 420 are different devices, reference may be made to fig. 27, that is, the heating element 410 is an independent heating device, and may be specially used for heating the reagent pipeline 440, and particularly, the reagent may be heated by the independent heating element 410 during the process of sucking, spitting and mixing the reagent by the hydrodynamic device 430. As shown in fig. 28, when the heat-generating body 410 and the container 420 are the same component, that is, the container 420 has a heat-conducting function due to the heat-generating body 410, and the reagent tube 440 is densely wound around the outer circumference of the container 420, thereby obtaining a heating effect.

In one embodiment, the heating element 410 is a separate heating cylinder, and the separate heating cylinder includes a winding tube base (refer to the heating cylinder 301) for winding the reagent conduit 440 and a heating element (refer to the heating rod 346) disposed in the winding tube base or attached to the outside of the reagent conduit 440. The heating element can be a heating rod inserted in the winding pipe base body, or the heating element is a heating film attached to the outer side of the pipeline.

The outer surface of the winding tube substrate is provided with a tube bundle structure, the tube bundle structure can be a tube penetrating channel (refer to the tube penetrating channel 302) or a tube clamping groove which is arranged on the surface of the winding tube substrate, the tube clamping groove is in a closed groove and is sunken on the surface of the winding tube substrate, and the tube bundle structure can be pressed and kept in the tube clamping groove through the deformation of the reagent pipeline 440.

The reagent tube 440 may be spirally wound in a cylindrical shape, or the reagent tube 440 may be spirally wound in a disc shape, or the reagent tube 440 may be wound in an S-shape, and when the reagent tube 440 is spirally wound in a disc shape or wound in an S-shape, the heating element 410 may be in a planar shape.

The container 420 may include an optical detection cell 120 and the reagent conduit 440 includes a diluent conduit for transporting diluent, the diluent conduit being disposed between the optical detection cell 120 and the hydrodynamic device 430. The container 420 may further include a blending tank, and the reagent conduit 440 includes an antibody reagent conduit for transporting an antibody reagent, the antibody reagent conduit being disposed between the blending tank and the hydrodynamic device 430. The container 420 may further comprise a pre-heat reservoir, and the reagent conduit 440 comprises a hemolysis reagent conduit for transporting a hemolysis reagent, the hemolysis reagent conduit being disposed between the pre-heat reservoir and the hydrodynamic device 430.

Referring to fig. 29, the detecting device may include a heating element 410, a container 420, a hydrodynamic device 430, a reagent tube 440, a reversing valve 450, a temperature control system 460, a waste liquid treating device 470, a reagent bottle 480, and the like.

The reagent pipeline 440 is connected between the container 420 and the fluid dynamic device 430, at least a part of the reagent pipeline 440 is wound and disposed on the heating element 410, for example, the pipeline C, D is wound and disposed on the heating element 410, the reagent pipeline 440 is used for conveying a reagent to the container 420 through the fluid dynamic device 430, the fluid dynamic device 430 is also used for sucking and spitting a blending reagent, the reagent is heated by the heating element 410 in the sucking and spitting blending process of the fluid dynamic device 430, the reagent is heated by the heating element 410 in the conveying process of the fluid dynamic device 430 to the container 420, the heating element 410 may be a heating barrel, and the temperature control system 460 may include a temperature sensor, a heating rod, a temperature control switch, and the like.

The reagent may be hemolysis reagent, antibody reagent, diluent, or mixture of two or three of them. Correspondingly, the number of the reagent bottles 480 is plural, and the reagent bottles 480 are respectively used for holding hemolysis reagent, antibody reagent, diluent and the like, the reagent bottles 480 are connected with the container 420 and the liquid power device 430 through the reversing valve 450, the number of the reversing valve 450 and the liquid power device 430 can be one or more, depending on the actual liquid path requirement, the hemolysis reagent and the antibody reagent can be heated by the heating element 410 when being transported to the container 420 in the reagent pipeline 440, the heating time is relatively short, the influence on the detection caused by the poor activity of the reagent due to long-time heating can be avoided, and simplified the structure, need not to set up corresponding preheating tank alone, the diluent can avoid relatively microthermal diluent to enter into the container 420 of relative high temperature and can reduce the temperature of container 420 through heat-generating body 410 heating when being carried to container 420 in reagent pipeline 440, can be convenient for next sample testing's quick the going on from this.

As shown in fig. 30A, point a is the starting temperature, point Bx (B1, B2, B3) is the CRP reaction temperature, and point Cx (C1, C2, C3) is the dilution wash finishing temperature. Starting from the normal temperature at point A in the absence of diluent line heating, it can be seen from B1- > B2- > B3 in the continuous measurement that the temperature becomes lower and lower in the continuous reaction and does not return to the state of the initial reaction temperature at the Cx point.

However, after the diluent pipeline is added for heating, the temperature of Cx can be restored to be close to the temperature range A every time, so that the reaction temperature Bx is also approximately maintained at 30 ℃ and the fluctuation range is within 0.5 ℃ under each continuous reaction, and the temperature control effect is remarkable.

Referring to fig. 30, the detecting apparatus includes a heating element 410, a container 420, a hydrodynamic device, at least one reversing valve, and a plurality of reagent pipes, wherein at least a portion of the reagent pipes are connected between the container 420 and the hydrodynamic device and wound around the heating element 410 to form a winding section; at least part of the reagent pipeline is used for sucking the reagent through the liquid power device and the reversing valve, and the reagent enters the winding section and is heated through the heating body 410 in the reagent sucking process of the liquid power device; or at least part of the reagent pipeline is used for pushing and sucking the reagent to the container 420 through the liquid power device and the reversing valve, and the reagent enters the winding section to be heated through the heating body 410 in the process of pushing and sucking the reagent by the liquid power device.

Wherein, heat-generating body 410 is an independent heating cylinder, an independent heating cylinder is including twining a tub base and heating member, twine a tub base and be used for supplying reagent pipeline winding, the heating member sets up in twining a tub base or pastes and establishes the reagent pipeline outside, the heating member is for inserting the heating rod who locates in twining a tub base, perhaps the heating member pastes the heating film of locating the section of convoluteing periphery for the cladding formula, the surface of twining a tub base is equipped with the bundle tubular construction, the bundle tubular construction is for locating the poling passageway or the card pipe groove of twining a tub base surface, the reagent pipeline can be the teflon pipe.

In one embodiment, the reagent pipeline comprises a first reagent suction pipeline G, a first reagent pushing pipeline BF and a first public pipeline I; the reversing valve comprises a first reversing valve 1; the hydrodynamic device comprises a first hydrodynamic device 431; a first common conduit I connects the common port of the first reversing valve 1 with the first hydrodynamic device 431; one end of the first reagent suction pipeline G is connected with one branch reversing port of the first reversing valve 1, the other end of the first reagent suction pipeline G is used for connecting a first reagent bottle, such as a reagent bottle containing diluent, and the first hydrodynamic device 431 sucks reagent through the first common pipeline I, the first reversing valve 1 and the first reagent suction pipeline G; first reagent propelling movement pipeline BF is around establishing on heat-generating body 410 and dividing another switching-over mouth with first switching-over valve 1 and being connected with container 420, first liquid power device 431 is through first public pipeline I, first switching-over valve 1 and first reagent propelling movement pipeline BF push away reagent or push away inhale reagent and reach and inhale and tell the mixing effect, this reagent is heated through heat-generating body 410 after getting into the winding section, inhale reagent or push away inhale the reagent operation in the first reagent bottle next time before, there is the reagent in the operation messenger first reagent propelling movement pipeline BF of previous time, this reagent has been heated in the void time before the next operation.

In one embodiment, the reagent pipeline comprises a second reagent suction pipeline H, a second reagent pushing pipeline and a second public pipeline E; the reversing valve comprises a second reversing valve 2; the hydrokinetic device comprises a second hydrokinetic device 432; a second common conduit E connects the common port of the second reversing valve 2 with a second hydraulic power means 432; one end of the second reagent suction pipeline H is connected with one branch reversing port of the second reversing valve 2, the other end of the second reagent suction pipeline H is used for connecting a second reagent bottle, such as a reagent bottle containing a hemolytic agent, and the second liquid power device 432 sucks the reagent through the second common pipeline E, the second reversing valve 2 and the second reagent suction pipeline H; the second reagent pushing pipeline is wound on the heating body 410 and connects the other branch reversing port of the second reversing valve 2 with the container 420, the second liquid power device 432 pushes or pushes the reagent through the second common pipeline E, the second reversing valve 2 and the second reagent pushing pipeline to achieve the effect of sucking, spitting and uniformly mixing, and the reagent is heated through the heating body 410 after entering the winding section. Wherein, the second reagent pushing pipeline can be a single pipe or a plurality of pipes in the following embodiments.

In one embodiment, the reagent lines include a second reagent extraction line H, a third reagent line extraction line J, a second common line E, a third common line CD, and a fourth common line a; the reversing valves comprise a second reversing valve 2 and a third reversing valve 3; the hydrokinetic device comprises a second hydrokinetic device 432; one end of the second reagent suction pipeline H is connected with one branch reversing port of the second reversing valve 2, the other end of the second reagent suction pipeline H is used for connecting a second reagent bottle, such as a reagent bottle containing a hemolytic agent, and the second liquid power device 432 sucks the reagent through the second common pipeline E, the second reversing valve 2 and the second reagent suction pipeline H; one end of a third reagent tube suction pipeline J is connected with one branch reversing port of the third reversing valve 3, and the other end of the third reagent tube suction pipeline J is used for connecting a third reagent bottle, such as a reagent bottle containing antibody liquid; a second common conduit E connects the common port of the second reversing valve 2 with a second hydraulic power means 432; the third common pipeline CD is wound on the heating body 410 and connects the other branch reversing port of the second reversing valve 2 with the common port of the third reversing valve 3; a third common conduit CD connects the other branch port of the third diverter valve 3 with the container 420.

The second liquid power device 432 pushes or pushes the reagent through the second common pipe E, the second reversing valve 2, the third common pipe CD, the third reversing valve 3 and the fourth common pipe a to achieve the effect of sucking, spitting and mixing the reagent, the reagent is heated by the heating element 410 after entering the winding section,

the second liquid power device 432 sucks reagent through the second common pipeline E, the second reversing valve 2, the third common pipeline CD, the third reversing valve 3 and the third reagent sucking pipeline J, at this time, the reagent enters the winding section and is heated by the heating body 410, since the operation of sucking or pushing the reagent in the second reagent bottle has been previously performed, the second common line E, the third common line CD, and the fourth common line a are filled with the reagent before the next operation, wherein the conduit relatively near the end of the second hydrodynamic device 432 is primarily filled with reagent from the second reagent bottle, therefore, when the reagent in the third reagent bottle is sucked, the reagent in the second reagent bottle near the end of the second hydrodynamic device 432 and the reagent in the third reagent bottle near the end of the third reversing valve 3 are contained in the third common pipeline CD, and the two reagents can be simultaneously heated in the winding section at the gap time before the next operation. The embodiment reduces the number of structural members through pipeline multiplexing, reversing valve multiplexing and heating body multiplexing, optimizes the pipeline layout, improves the temperature control effect of the reagent, can reduce the temperature fluctuation of the container 410, and improves the detection stability. The present embodiment also provides a sample analyzer, which includes the aforementioned detection device. The utility model provides an in the scheme, reagent is heated through heat-generating body 410 in the mixing in-process is told in inhaling of hydrodynamic device 430, can heat and keep warm to the reagent in reagent pipeline 440, has improved the detection precision.

The ninth embodiment, the embodiment of the utility model provides a specific protein detection module, this specific protein detection module includes hydraulic power device, heating device and multichannel detection liquid way, every detection liquid way all includes preheating the pond, mixing pond (X1, X2, X3), determine module, first pipeline T182, second pipeline T99, third pipeline T102, fourth pipeline T101, fifth pipeline T39 all the way, can also add sixth pipeline T98 in every way detection liquid according to actual need. The plurality of detection liquid paths include at least two detection liquid paths, and the following description will be made with reference to three detection liquid paths shown in fig. 31 to 33.

The liquid power device is used for carrying out reagent providing operation and sucking, spitting and mixing operation; the heating device is used for heating at least one of the blending pool (X1, X2 and X3), the detection assembly, the second pipeline T99, the third pipeline T102, the fourth pipeline T101, the fifth pipeline T39 and the sixth pipeline T98; a first pipe T182 connects the waste liquid collecting device WC1 with the kneading tank (X1, X2, X3) for discharging waste liquid to the waste liquid collecting device WC; the second pipeline T99 is connected with the blending pool (X1, X2 and X3) and is used for providing a hemolysis reagent to the blending pool (X1, X2 and X3) through a liquid power device; the third pipeline T102 is connected with the blending pool (X1, X2, X3) and is used for providing antibody reagent to the blending pool (X1, X2, X3) through a liquid power device; the fourth pipeline T101 connects the blending pool (X1, X2 and X3) with the liquid power device and is used for carrying out suction and discharge blending operation through the liquid power device; the detection assembly is used for detecting the uniformly mixed reagent, the uniformly mixing pool (X1, X2 and X3) and the detection pool in the detection assembly are arranged integrally or separately (the integrally arrangement means that the uniformly mixing pool is cancelled and the detection pool in the detection assembly is directly used for carrying out the uniformly mixing function), and the preheating pool is connected to the second pipeline and is used for preheating the hemolytic reagent; the fifth pipeline T39 is connected with the fourth pipeline T101 and is used for providing diluent for the blending pool (X1, X2 and X3) and the detection assembly through a hydraulic power device; the sixth pipeline T98 is connected with the fourth pipeline T101 and is used for providing cleaning liquid to the blending pool (X1, X2 and X3) and the detection assembly through a hydraulic power device.

Specifically, the hydrodynamic device includes a plurality of types, and the types of the hydrodynamic device may be a fixed displacement pump, a syringe, and/or an air source, etc., and is connected to the detection liquid path through an electromagnetic valve, for example, the fixed displacement pumps DP04, DP05, DP06 for the hemolysis reagent supplying operation in fig. 31 and 32, the fixed displacement pumps DP01, DP02, DP03 for the antibody reagent supplying operation, a pressure source for connecting the dilution liquid tank DIL-MR for the dilution liquid supply, the fixed displacement pump DP07 for the cleaning liquid supply, a kneading syringe for the suction and discharge kneading operation, the hemolysis reagent syringe 1, the hemolysis reagent syringe 2, and the hemolysis reagent syringe 3 for the hemolysis reagent supplying operation in fig. 33, the anti-body fluid syringe 1, the anti-body fluid syringe 2, and the anti-body fluid syringe 3 for the antibody reagent supplying operation, the kneading syringe IR for the dilution liquid supply, and the cleaning liquid supply syringe for the cleaning liquid supply, in the embodiment shown in fig. 33, the mixing injector IR is reused for the diluent supply operation and the sucking, spitting and mixing operation, and the SV16 valve is added in the liquid path, so that the use of the injector can be reduced, the volume of the product can be reduced, and the product cost can be reduced. It will be readily understood by those skilled in the art that the type and use of the liquid power apparatus are not limited to those shown in FIGS. 31 to 33, and those skilled in the art may arbitrarily combine the use of the metering pumps DP04, DP05, DP06 for the hemolysis reagent supplying operation in FIG. 31 with a syringe, the use of the metering pumps DP01, DP02, DP03 for the antibody reagent supplying operation in FIG. 32 with a syringe, the use of the cleaning solution syringe for the cleaning solution supplying operation in FIG. 33 with a metering pump, and the like.

The number of the liquid power devices for carrying out suction and discharge blending operation is N, and the number of the multi-path detection liquid paths is N. When n is 1, the mixing operation of the multi-channel detection liquid path is multiplexed with the same hydrodynamic device, for example, as shown in fig. 31, and the mixing operation of the 3-channel detection liquid path is multiplexed with the mixing syringe IR. When N is equal to N, one hydrodynamic device is used for each of the sucking, discharging and kneading operations of the detection liquid paths, and as shown in fig. 32, the kneading syringe IR1, the kneading syringe IR2 and the kneading syringe IR3 are used for each of the sucking, discharging and kneading operations of the 3-path detection liquid path. When 1< N, the suction, discharge, and mixing operations of at least a part of the detection liquid paths are performed by multiplexing the same liquid power device, for example, when N is 6 and N is 3, the liquid power device for performing the suction, discharge, and mixing operations may be divided into two detection liquid paths. Or the first liquid power device for absorbing, spitting and mixing operation detects the liquid path in one way, the second liquid power device for absorbing, spitting and mixing operation detects the liquid path in one way, and the third liquid power device for absorbing, spitting and mixing operation detects the liquid path in four ways. Or the first liquid power device for absorbing, spitting and mixing operation is used for detecting one liquid path, the second liquid power device for absorbing, spitting and mixing operation is used for detecting two liquid paths, and the third liquid power device for absorbing, spitting and mixing operation is used for detecting three liquid paths.

The multi-path detection liquid path can be used for detecting the same specific protein, and the specific protein can be C-reactive protein, serum amyloid A or transferrin; or multiple detection paths for detecting different specific proteins, e.g., in one embodiment, at least one detection path for detecting C-reactive protein (CRP) and at least one other detection path for detecting Serum Amyloid A (SAA).

The embodiment of the utility model provides an in, heating device is a plurality of heating element that the differentiation set up including the temperature, and a plurality of heating element are used for carrying out independent control by temperature change or combination control by temperature change to preheating pond, mixing pond (X1, X2, X3), determine module, second pipeline T99, third pipeline T102, fourth pipeline T101 and/or fifth pipeline T39. The independent temperature control can be understood as how many objects to be heated have the same number of heating assemblies, and the objects to be heated are independently heated in a one-to-one correspondence manner, and the combined temperature control can be understood as that at least two heating objects share one heating assembly, for example, the temperatures of a preheating pool and a blending pool (X1, X2 and X3) are relatively close to each other, and the same heating assemblies can be reused.

The heating assembly is a heating film or a heating rod, the specific protein detection module further comprises a plurality of heat-conducting substrates for conducting heat to the preheating pool, the uniformly mixing pool (X1, X2 and X3), the detection assembly, a second pipeline T99, a third pipeline T102, a fourth pipeline T101 and/or a fifth pipeline T39, the heat-conducting substrates are in a block shape or a cylinder shape, the heating film is attached to the heat-conducting substrates, or the heating rod is inserted in the heat-conducting substrates, and the second pipeline T99, the third pipeline T102, the fourth pipeline T101 and/or the fifth pipeline T39 are wound on the cylindrical heat-conducting substrates.

In an embodiment, wherein, heating device includes the diluent heating bath, and the diluent heating bath passes through pipeline T120, pipeline T83 and connects on fifth pipeline T39 and be parallelly connected the setting with the relative fourth pipeline T101 of hydrodynamic device who is used for carrying on inhaling to spit the mixing operation, and this setting mode can make the bubble that the diluent heating bath heating produced can not influence the detection precision, is favorable to improving the overall stability who detects.

The specific protein detection module comprises a substrate, a detection pool arranged on the substrate, and a laser and a signal receiver which are arranged on the substrate and positioned at two sides of the detection pool, wherein the detection pool is communicated with a mixing pool (X1, X2 and X3).

Or the specific protein detection module comprises a substrate, a laser and a signal receiver, the blending pool (X1, X2 and X3) is arranged on the substrate, and the laser and the signal receiver are arranged on the substrate and positioned at two sides of the blending pool (X1, X2 and X3).

As shown in fig. 31 to 33, the specific protein detection module further includes a plurality of first gate valves (SV10, SV11, SV12), a plurality of second gate valves (SV04, SV05, SV06), a plurality of third gate valves (SV01, SV02, SV03), a plurality of bus joints (J2, J3, J3), and a bus line T199.

The first pipeline T182 connects the blending pools (X1, X2 and X3) with confluence joints (J2, J3 and J3), the first option valves (SV10, SV11 and SV12) are connected to the first pipeline T182, and the confluence pipeline T199 connects a plurality of confluence joints (J2, J3 and J3) in series to a waste liquid collecting device WC 1.

The second pipeline comprises a second common pipeline T106, second liquid pushing pipelines (T97, T99) and second liquid absorbing pipelines (T95, T94), the second common pipeline T106 connects the common end of the second selection valve (SV04, SV05, SV06) with a liquid power device (quantitative pumps DP04, DP05, DP 69528) for providing reagent, the second liquid pushing pipelines (T97, T99) connect one branch port of the second selection valve (SV04, SV05, SV06) with a mixing pool (X1, X637, X3), the second liquid pushing pipelines (T97 ) are connected with a preheating pool, the second liquid absorbing pipelines (T97 ) are connected with the other branch ports of the second selection valve (SV 97, SV 72, SV 97) and connected with a hemolytic reagent group, and the second liquid absorbing pipelines (T97 ) are provided with optical coupling detection paths for detecting no-liquid.

The third pipeline comprises a third common pipeline T106, a third liquid pushing pipeline T102 and a third liquid suction pipeline T103, the third common pipeline T106 connects the common end of the third selective valve (SV01, SV02 and SV03) and a liquid power device (quantitative pumps DP01, DP02 and DP03) for supplying the reagent, the third liquid pushing pipeline T102 connects one branch port of the third selective valve (SV01, SV02 and SV03) with the mixing pool (X1, X2 and X3), the third liquid suction pipeline T103 is connected with the other branch port of the third selective valve (SV01, SV02 and SV03) and is connected with an antibody liquid reagent bottle, and the optical coupling path of the third liquid suction pipeline T103 is provided with a liquid presence or absence detection.

As shown in fig. 31, the specific protein detection module further includes a fourth selective valve SV09, a fifth selective valve SV08, a sixth selective valve SV07, a plurality of seventh selective valves (SV10, SV11, SV12), a first three-way joint J3, a second three-way joint J3, and a plurality of shunt joints (J3, J3).

The sixth pipeline comprises a sixth first pipeline T106, a sixth second pipeline T98 and a sixth third pipeline (T95, T94), the sixth first pipeline T106 connects the common end of the sixth selective valve SV07 with a liquid power device (a quantitative pump DP07) for providing a reagent, the sixth second pipeline T98 is connected between one branch port of the sixth selective valve SV07 and the first port of the first three-way joint J3, the sixth third pipeline (T95, T94) is connected with the other branch port of the sixth selective valve SV07 and connected with a cleaning liquid reagent bottle, and optical couplers are arranged on the sixth third pipeline (T95, T94) for detecting whether the liquid exists or not.

The fifth pipeline comprises a fifth first pipeline T39, fifth second pipelines (T120 and T83) and a fifth third pipeline T12, the fifth first pipeline T39 connects the common end of the fifth selective valve SV08 with the diluent tank DIL-MR, the fifth second pipelines (T120 and T83) are connected between one branch port of the fifth selective valve SV08 and the second port of the first three-way joint J3, the fifth third pipeline T12 is connected between the other branch port of the fifth selective valve SV08 and the first port of the second three-way joint J3, and the second port of the second three-way joint J3 is connected with a liquid power device (a blending injector IR) for absorbing and spitting.

The fourth pipeline comprises a fourth first pipeline T100, a fourth second pipeline T1 and a fourth third pipeline T69, the fourth first pipeline T100 connects the common end of the fourth selective valve SV09 with a plurality of blending pools (X1, X2 and X3) through a plurality of shunt joints (J3 and J3), a plurality of seventh selective valves (SV10, SV11 and SV12) are connected on the pipelines between the blending pools (X1, X2 and X3) and the shunt joints (J3 and J3), the fourth second pipeline T1 is connected between one branch port of the fourth selective valve SV09 and the third port of the first tee joint J3, and the fourth third pipeline T69 is connected between the other branch port of the fourth selective valve SV09 and the third port of the second tee joint J3.

As shown in fig. 32, the specific protein detection module further includes a fourth gate valve (SV10, SV11, SV12), a fifth gate valve SV08, a sixth gate valve SV07, a plurality of seventh gate valves (SV10, SV11, SV12), a first three-way joint J3, a plurality of second three-way joints J3, and a plurality of shunt joints (J3, J3).

The sixth pipeline includes a sixth first pipeline T106, a sixth second pipeline T98 and a sixth third pipeline (T95, T94), the sixth first pipeline T106 connects the common end of the sixth selection valve SV07 with the fluid power device (fixed displacement pump DP07) for providing the reagent, the sixth second pipeline T98 is connected between one branch port of the sixth selection valve SV07 and the first port of the first three-way joint J3, and the sixth third pipeline (T95, T94) is connected with the other branch port of the sixth selection valve SV 07.

The fifth pipeline connects the diluent tank DIL-MR with the second port of the first three-way joint J3, and the fifth option valve SV08 is connected to the fifth pipeline.

Three ports of the second three-way joints J3 are respectively connected with the mixing pool (X1, X2 and X3), a liquid power device (mixing injector IR1, mixing injector IR2 and mixing injector IR3) for absorbing and discharging mixing, and one end of a fourth selective valve (SV10, SV11 and SV12), and the other end of the fourth selective valve (SV10, SV11 and SV12) is connected with the third port of the first three-way joint J3 through a plurality of shunt joints (J3 and J3).

As shown in fig. 33, the specific protein detection module further includes a fourth selective valve SV09, a fifth selective valve SV08, a sixth selective valve SV07, a plurality of seventh selective valves (SV10, SV11, SV12), a first three-way joint J3, and a shunt joint (J3, J3).

The sixth pipeline comprises a sixth first pipeline T106, a sixth second pipeline T98 and a sixth third pipeline (T95, T94), the sixth first pipeline T106 connects the common end of the sixth selective valve SV07 with a liquid power device (a quantitative pump DP07) for providing a reagent, the sixth second pipeline T98 is connected between one branch port of the sixth selective valve SV07 and the first port of the first three-way joint J3, the sixth third pipeline (T95, T94) is connected with the other branch port of the sixth selective valve SV07, and optical couplers are arranged on the sixth third pipeline (T95, T94) to detect whether liquid exists or not.

The fifth pipeline comprises a fifth first pipeline T39, fifth second pipelines (T120 and T83) and a fifth third pipeline T12, the fifth first pipeline T39 connects the common end of the fifth selective valve SV08 with a liquid power device (blending injector IR) for performing suction and discharge blending, the fifth second pipelines (T120 and T83) are connected between one branch port of the fifth selective valve SV08 and the second port of the first three-way joint J3, the fifth third pipeline T12 is connected between the other branch port of the fifth selective valve SV08 and one branch port of the fourth selective valve SV09, and the third port of the first three-way joint J3 is connected with the other branch port of the fourth selective valve SV 09.

The common end of the fourth selective valve SV09 is connected with the blending pools (X1, X2 and X3) through a plurality of shunt joints (J3 and J3), and a plurality of seventh selective valves (SV10, SV11 and SV12) are connected on pipelines between the blending pools (X1, X2 and X3) and the shunt joints (J3 and J3).

An expansion pipeline T101 is arranged between the seventh selective valve (SV10, SV11 and SV12) and the detection assembly, the expansion pipeline T101 can increase the buffer volume in a way of expanding the pipeline diameter or increasing the pipeline length (such as multi-turn winding) so as to prevent the blending reagent from exceeding the shunt joint (J3 and J3) when absorbing, spitting and blending, preferably not exceeding the seventh selective valve (SV10, SV11 and SV12), and if the blending reagent exceeds the shunt joint (J3 and J3), the blending reagent in the liquid path can enter the pipeline T100 and the pipeline T184 to enter other liquid paths in subsequent liquid pushing so as to influence the detection accuracy of other detection liquid paths.

The embodiment of the utility model also provides a blood detection instrument, which comprises an electrically connected control analysis module, a collection and distribution module, a blood routine measurement module and the specific protein detection module; the collection and distribution module is used for collecting blood samples and distributing the blood samples to the blood routine measurement module and the specific protein detection module; the specific protein detection module is used for testing specific protein parameters of the blood sample, such as CRP, SAA, PCT, D-Dimer, TRF, Hs-CRP, ADPN and the like; the blood routine measurement module is used for testing blood routine parameters of the blood sample. Wherein, gather and the sampling needle of distribution module still can be used to carry out reagent and add, avoids reagent to persist and deteriorate in the pipeline for a long time, practices thrift reagent cost, and perhaps, gather and the distribution module still can include the reagent needle, and the reagent needle can be used to carry out the absorption and the interpolation of reagent.

Referring to fig. 31, the specific flow of detection in the liquid path (hereinafter referred to as channel 1) where the blending pool X1 is located is as follows:

step 1: opening an SV13 valve, emptying the original base solution (the liquid for soaking the blending pool X1, which can be diluent) in the blending pool X1, controlling a quantitative pump DP06 and an SV06 valve, adding a specific protein hemolysis reagent into the blending pool X1 to clean the blending pool X1, opening an SV13 valve again and emptying the blending pool X1;

step 2: controlling a quantitative pump DP06 and an SV06 valve, adding a specific protein hemolysis reagent into a mixing pool X1, and simultaneously controlling a sampling needle of an acquisition and distribution module to add a blood sample;

and step 3: opening an SV08 valve, an SV09 valve and an SV10 valve, controlling an IR (infrared) mixing injector to suck and spit back and forth, fully mixing a hemolytic reagent and a blood sample, and keeping the temperature at the position of a mixing pipeline T101 through a heating device, for example, a cylindrical heat-conducting substrate with a heating rod arranged inside is adopted, and the mixing pipeline T101 is wound on the periphery of the cylindrical heat-conducting substrate, so that unstable reaction temperature caused by heat loss in the mixing process is avoided;

and 4, step 4: after the hemolysis process is finished, controlling a quantitative pump DP01 and an SV01 valve, adding a specific protein antibody reagent into a mixing pool X1, then opening an SV08 valve, an SV09 valve and an SV10 valve, controlling a mixing injector IR to suck and spit back and forth, fully mixing reaction liquid, and similarly, adding a heating device at the position of a mixing pipeline T101 to keep the temperature, so as to avoid unstable reaction temperature caused by heat dissipation in the mixing process;

and 5: after the complete mixing, opening an SV08 valve, an SV09 valve and an SV10 valve, controlling a mixing injector IR to send the reaction liquid into a detection cell of a detection assembly, and starting detection;

step 6: after the detection is finished, opening an SV10 valve, controlling a constant delivery pump DP07 and an SV07 valve, and adding cleaning liquid into pipelines T98, T1, T100, T101 and T104;

and 7: opening an SV08 valve and an SV10 valve, communicating the diluent tank DIL-MR to the reaction channel 1, opening an SV22 valve, enabling positive pressure to enter the diluent tank DIL-MR, feeding diluent liquid into pipelines T39, T120 and T83 to further push cleaning liquids in pipelines T1, T100, T101 and T104 to clean the blending pipeline T101, the detection assembly and the blending pool X1 together, because the diluent pushes the cleaning liquid to enter the detection assembly and the mixing pool X1 in sequence, compared with the cleaning mode that the sampling needle is used for adding the cleaning liquid into the mixing pool X1, the mixing injector IR is used for pumping the cleaning liquid from the mixing pool X1 into the detection pool for cleaning, and finally the liquid in the detection pool is pushed back to the mixing pool X1, the cleaning mode has the advantages of simple operation, short cleaning time and strong cleaning effect, wherein the cleaning strength of the cleaning solution to the specific substance is greater than that of the diluent to the specific substance, and the specific substance may be a combination of immunoreaction or a reaction residue. Opening SV13 to evacuate a mixing pool X1 after cleaning, and finally adding diluent into the mixing pool X1 to form base solution; the diluent of entering needs to pass through the heating pond between SV08 valve and tee bend J3, and the purpose is the heating diluent, makes the liquid temperature who gets into the detection pond keep at a scope, avoids detecting the too big influence reaction stability of pond difference in temperature, when examining in succession, because the diluent has been heated, the liquid temperature who gets into in the detection pond during the washing can not reduce by a wide margin, and convenient next time detects and can begin fast.

The specific flow of counting detection in the liquid paths (which can be called channels 2 and 3) where the mixing pool X2 and the mixing pool X3 are located is the same as the detection flow in the channel 1, when a plurality of channels detect the same specific protein, the reagent in the access pipeline is the same to generate the same chemical reaction, and when a plurality of channels detect different specific proteins, the reagent in the access pipeline is different to generate different chemical reactions.

Referring to fig. 32, the specific flow of counting detection in the liquid path (hereinafter referred to as channel 1) where the blending pool X1 is located is as follows:

and step 3: starting the blending injector IR1 to suck and spit back and forth, fully blending the hemolytic reagent and the blood sample, and keeping the temperature at the position of the blending pipeline T101 through a heating device, for example, a cylindrical heat-conducting substrate with a heating rod arranged inside is adopted, and the blending pipeline T101 is wound around the periphery of the cylindrical heat-conducting substrate, so that unstable reaction temperature caused by heat dissipation in the blending process is avoided;

and 4, step 4: after the hemolysis process is finished, controlling a quantitative pump DP01 and an SV01 valve, adding a specific protein antibody reagent into a mixing pool X1, starting a mixing injector IR1 to suck and spit back and forth, fully mixing reaction liquid, and similarly, adding a heating device at the position T101 of a mixing pipeline to keep the temperature, so as to avoid unstable reaction temperature caused by heat loss in the mixing process;

and 5: after the complete mixing is finished, starting the mixing injector IR1 to suck and spit back and forth to send the reaction liquid into the detection cell of the detection assembly, and starting the detection;

step 6: after the detection is finished, opening an SV10 valve, controlling a constant delivery pump DP07 and an SV07 valve, and adding cleaning liquid into pipelines T98, T100, T101 and T104; opening SV08 valve, SV10 valve and SV17 valve, making the positive pressure enter diluent tank DIL-MR, and putting the diluent liquid into pipelines T39, T120 and T83 to push the cleaning liquid in pipelines T100, T101 and T104 to clean the blending pipeline T101, the detection component and the blending pool X1, because the diluent liquid pushes the cleaning liquid to enter the detection component and the blending pool X1 in sequence, compared with the cleaning method that the sampling needle adds the cleaning liquid into the blending pool X1, the blending syringe pumps the cleaning liquid from the blending pool X1 to the detection pool for cleaning and finally pushes the liquid in the detection pool back to the blending pool X1, the operation of the cleaning method is simple, the cleaning time is shortened, and the cleaning effect is enhanced, wherein the cleaning strength of the cleaning liquid to the specific substance is greater than that of the diluent liquid to the specific substance, and the specific substance can be a combination of immunoreaction or a reaction residue. Opening SV13 to evacuate a mixing pool X1 after cleaning, and finally adding diluent into the mixing pool X1 to form base solution; the entering diluent needs to pass through a heating pool between an SV08 valve and a tee joint J3, the purpose is to heat the diluent, so that the temperature of the liquid entering a detection pool is kept in a range, and the influence of the overlarge temperature difference of the detection pool on the reaction stability is avoided.

Referring to fig. 33, the specific flow of counting detection in the liquid path (hereinafter referred to as channel 1) where the blending pool X1 is located is as follows:

step 1: opening an SV13 valve, emptying the original base solution (the liquid for soaking the blending pool X1, which can be diluent) in the blending pool X1, controlling a hemolytic agent injector 1 and an SV06 valve, adding a specific protein hemolytic reagent into the blending pool X1 to clean the blending pool X1, opening an SV13 valve again and emptying the blending pool X1;

step 2: controlling a hemolytic agent injector 1 and an SV06 valve, adding a specific protein hemolytic reagent into the mixing pool X1, and simultaneously controlling a sampling needle of the acquisition and distribution module to add a blood sample;

and step 3: opening an SV16 valve, an SV09 valve and an SV10 valve, controlling an IR (infrared) mixing injector to suck and spit back and forth, fully mixing a hemolytic reagent and a blood sample, and keeping the temperature at the position of a mixing pipeline T101 through a heating device, for example, a cylindrical heat-conducting substrate with a heating rod arranged inside is adopted, and the mixing pipeline T101 is wound on the periphery of the cylindrical heat-conducting substrate, so that unstable reaction temperature caused by heat loss in the mixing process is avoided;

and 4, step 4: after the hemolysis process is finished, controlling an antibody liquid injector 1 and an SV01 valve, adding a specific protein antibody reagent into a mixing pool X1, then opening an SV16 valve, an SV09 valve and an SV10 valve, controlling an IR (infrared) of a mixing injector to suck and spit back and forth, fully mixing reaction liquid, and similarly, preserving heat through a heating device at the T101 position of a mixing pipeline, so that unstable reaction temperature caused by heat loss in the mixing process is avoided;

and 5: after the complete mixing, opening an SV16 valve, an SV09 valve and an SV10 valve, controlling a mixing injector IR to send the reaction liquid into a detection pool, and starting detection;

step 6: after the detection is finished, opening an SV10 valve, controlling a cleaning liquid injector and an SV07 valve, and adding cleaning liquid into pipelines T98, T1, T100, T101 and T104; opening an SV08 valve, an SV10 valve and an SV16 valve, controlling the blending injector IR to enable diluent in a diluent pool DIL to enter pipelines T39, T120 and T83 so as to push cleaning liquids in pipelines T1, T100, T101 and T104 to clean the blending pipeline T101, the detection assembly and the blending pool X1 together, opening SV13 after cleaning to empty the blending pool X1, because the diluent pushes the cleaning liquid to enter the detection assembly and the mixing pool X1 in sequence, compared with the cleaning mode that the sampling needle is used for adding the cleaning liquid into the mixing pool X1, the mixing injector IR is used for pumping the cleaning liquid from the mixing pool X1 into the detection pool for cleaning, and finally the liquid in the detection pool is pushed back to the mixing pool X1, the cleaning mode has the advantages of simple operation, short cleaning time and strong cleaning effect, wherein the cleaning strength of the cleaning solution to the specific substance is greater than that of the diluent to the specific substance, and the specific substance may be a combination of immunoreaction or a reaction residue. Opening SV13 to evacuate a mixing pool X1 after cleaning, and finally adding diluent into the mixing pool X1 to form base solution; the entering diluent needs to pass through a heating pool between an SV08 valve and a tee joint J3, the purpose is to heat the diluent, so that the temperature of the liquid entering a detection pool is kept in a range, and the influence of the overlarge temperature difference of the detection pool on the reaction stability is avoided.

The utility model also provides a method for blood detection based on the blood detection instrument, the detection liquid path is used for detecting at least two specific protein parameters, and at least one of the detection liquid paths is used for detecting CRP; at least one other detection liquid path is used for detecting SAA; the blood routine measuring module comprises a multi-path measuring liquid path; one measuring liquid path is used for measuring WBC; wherein, one measuring liquid path is used for measuring RBC; one of the measuring liquid paths is used for measuring DIFF.

Specifically, the method for detecting CRP and SAA further comprises the following steps:

emptying the mixing pool and the detection assembly of the CRP detection liquid path and the SAA detection liquid path, and then adding a first hemolytic agent into the mixing pool of the CRP detection liquid path and the mixing pool of the SAA detection liquid path through a liquid power device.

And moving the acquisition and distribution module to the upper part of the mixing pool of the CRP detection liquid path, adding part of the blood sample into the mixing pool of the CRP detection liquid path, and fully mixing the blood sample once through a liquid power device.

And moving the acquisition and distribution module to the upper part of the mixing pool of the SAA detection liquid path, adding part of blood samples into the mixing pool of the SAA detection liquid path, and fully mixing the blood samples once through a liquid power device.

And (2) absorbing a quantitative first antibody reagent from a reagent bottle in the refrigerating chamber through a liquid power device, adding the reagent into a mixing pool of a CRP detection liquid path, fully mixing for the second time through the liquid power device, absorbing the reagent into a detection assembly through the liquid power device after mixing, detecting the CRP, and outputting a measurement result.

Absorb quantitative second antibody reagent from the reagent bottle internal absorption in the freezer through liquid power device, then add in the mixing pond of SAA detection liquid way to carry out the abundant mixing of secondary through liquid power device, inhale through liquid power device after the mixing and carry out SAA detection and output measuring result in the detection subassembly.

The CRP and SAA can be detected by using the same hemolytic agent.

And moving the collecting and distributing die to a cleaning cup position, cleaning by using a cleaning solution and a diluent, and recovering to an instrument preparation state.

And after the CRP detection is finished, emptying the residual sample in the mixing pool of the CRP detection liquid path, and adding a cleaning solution and a diluent into the mixing pool of the CRP detection liquid path through a hydrodynamic device for cleaning so as to restore the CRP detection liquid path to an instrument preparation state.

And after the SAA detection is finished, emptying the residual sample in the mixing pool of the SAA detection liquid path, and adding cleaning liquid and diluent into the mixing pool of the CRP detection liquid path through a hydraulic power device for cleaning so as to restore the SAA detection liquid path to an instrument preparation state.

Further, the blood routine measurement module includes a first measurement fluid circuit including a measurement assembly, a WBC reaction cell, the method further including:

the WBC reaction cell is drained and then a diluent is added to the WBC reaction cell.

And moving the collecting and distributing die to the upper part of the WBC reaction pool, adding part of the blood sample into the WBC reaction pool, and uniformly mixing.

And adding a second hemolytic agent into the WBC reaction tank, uniformly mixing, detecting hemoglobin and leucocyte Baso, and outputting a detection signal.

And after the detection is finished, emptying the waste liquid, and cleaning the WBC reaction tank by using diluent so as to restore the first measurement liquid path to an instrument preparation state.

Further, the blood routine measurement module comprises a second measurement fluid path, the second measurement fluid path comprises a measurement assembly and an RBC reaction cell, and the method further comprises:

the RBC reaction cell is evacuated and a diluent is added to the RBC reaction cell.

And moving the collection and distribution module to the upper part of the RBC reaction tank, adding part of the blood sample into the RBC reaction tank, uniformly mixing, carrying out RBC and PLT detection, and outputting a detection signal.

And after the detection is finished, emptying the waste liquid, and cleaning the RBC reaction tank by using diluent to restore the second measurement liquid path to the instrument preparation state.

Further, the blood routine measurement module comprises a third measurement liquid path, the third measurement liquid path comprises a measurement component and a DIFF reaction pool, and the method further comprises the following steps:

and adding a third hemolytic agent into the DIFF reaction pool.

The collection and distribution die is moved over the DIFF reaction cell and a portion of the blood sample is added to the DIFF reaction cell.

And adding a fourth hemolytic agent into the DIFF reaction pool, uniformly mixing, performing DIFF detection and outputting a detection signal.

And after the detection is finished, emptying the waste liquid, and cleaning the DIFF reaction tank and the third measuring device by using diluent to restore the third measuring liquid path to the instrument preparation state.

The second hemolytic agent, the third hemolytic agent and the fourth hemolytic agent may be the same or different, and are selected according to the corresponding detection items.

It is worth mentioning that the cleaning solution has strong acidity, can thoroughly remove protein attached by reaction, and then removes the cleaning solution through the diluent, so that the background voltage of the detection assembly can be ensured to be clean and close to an ideal state.

The utility model provides a specific protein detection module, after the conventional measuring module of combination blood, can adopt a sample, detect the parameter of conventional and multiple specific protein project of blood simultaneously in same blood detecting instrument, revise specific protein parameter through the HCT parameter, realize the whole blood test, convenient operation improves the holistic detection efficiency of hospital.

Claims

1. A specific protein detection module is characterized by comprising a hydrodynamic device and a plurality of detection liquid paths, wherein each detection liquid path comprises a mixing pool, a detection assembly, a first pipeline, a second pipeline, a third pipeline and a fourth pipeline;

the fourth pipeline connects the blending pool with the liquid power device and is used for carrying out suction, discharge and blending operation through the liquid power device;

the detection assembly is used for detecting the uniformly mixed reagent.

2. The specific protein detection module according to claim 1, wherein: the specific protein detection module further comprises a heating device, and the heating device is used for heating at least one of the blending pool, the detection assembly, the second pipeline, the third pipeline and the fourth pipeline.

3. The specific protein detection module according to claim 2, wherein: the specific protein detection module further comprises:

the preheating tank is connected to the second pipeline and is used for preheating the hemolysis reagent;

and the fifth pipeline is connected with the fourth pipeline and used for providing diluent for the blending pool and the detection assembly through the hydraulic power device.

4. The specific protein detection module according to claim 3, further comprising a sixth pipeline connected to the fourth pipeline for providing a cleaning solution to the blending tank and the detection assembly via the hydrodynamic device.

5. The specific protein detection module according to claim 3, wherein the heating device comprises a plurality of heating components with different temperatures, and the plurality of heating components are used for independently controlling or combining the temperature of the preheating tank, the blending tank, the detection component, the second pipeline, the third pipeline, the fourth pipeline and/or the fifth pipeline.

6. The specific protein detection module according to claim 5, wherein the heating assembly is a heating film or a heating rod, the specific protein detection module further comprises a plurality of heat-conducting substrates, the heat-conducting substrates are in a block shape or a cylinder shape, the heating film is attached to the heat-conducting substrates, or the heating rod is inserted into the heat-conducting substrates, and the second pipeline, the third pipeline, the fourth pipeline and/or the fifth pipeline are wound on the cylindrical heat-conducting substrates.

7. The specific protein detection module according to claim 1, wherein:

the multiple detection liquid paths are used for detecting the same specific protein, and the specific protein is C-reactive protein, serum amyloid A or transferrin; or

And the multiple detection liquid paths are used for detecting different specific proteins, wherein at least one detection liquid path is used for detecting C-reactive protein, and at least another detection liquid path is used for detecting serum amyloid A.

8. The specific protein detection module according to claim 1, wherein the liquid power device is a quantitative pump, a syringe and/or an air source, and is connected to the detection liquid path through an electromagnetic valve, wherein the number of the liquid power devices for performing the sucking, spitting and mixing operation is N, the number of the multiple detection liquid paths is N,

when n is 1, the same liquid power device is multiplexed by the absorbing, discharging and mixing operations of the multiple detection liquid paths;

when N is equal to N, the liquid power device is independently used for the sucking, spitting and mixing operation of each detection liquid path;

and when N is more than 1 and less than N, at least part of the sucking, spitting and mixing operations of the detection liquid path multiplex the same liquid power device.

9. The specific protein detection module according to claim 3, wherein: the heating device comprises a diluent heating pool, the diluent heating pool is connected to the fifth pipeline and is used for sucking, spitting and uniformly mixing the fifth pipeline, and the liquid power device is opposite to the fourth pipeline and is arranged in parallel.

10. The specific protein detection module according to claim 1, wherein:

the specific protein detection module comprises a matrix, a detection pool arranged on the matrix, and a laser and a signal receiver which are arranged on the matrix and positioned at two sides of the detection pool, wherein the detection pool is communicated with the blending pool; or

The specific protein detection module comprises a base body, a laser and a signal receiver, wherein the mixing pool is arranged on the base body, and the laser and the signal receiver are arranged on the base body and positioned on two sides of the mixing pool.

11. The specific protein detection module according to claim 1, further comprising a plurality of first gate valves, a plurality of second gate valves, a plurality of third gate valves, a plurality of confluence joints, and a confluence pipeline;

the first pipeline connects the blending pool with the confluence joint, the first gate valve is connected on the first pipeline, and the confluence pipeline connects a plurality of confluence joints in series;

the second pipeline comprises a second public pipeline, a second liquid pushing pipeline and a second liquid sucking pipeline, the second public pipeline connects the public end of the second option valve with the liquid power device for providing the reagent, the second liquid pushing pipeline connects one branch port of the second option valve with the mixing pool, the second liquid sucking pipeline is connected with the other branch port of the second option valve, and an optical coupler is arranged on the path of the second liquid sucking pipeline;

the third pipeline includes third public pipeline, third liquid pushing pipeline and third imbibition pipeline, the third public pipeline will the public end of third option valve with be used for carrying on reagent and provide the hydrodynamic force device connects, the third liquid pushing pipeline will a branch port of third option valve with the mixing pond is connected, the third imbibition pipeline with another branch port of third option valve is connected, be equipped with the opto-coupler on the route of third imbibition pipeline.

12. The specific protein detection module according to claim 4, further comprising a fourth gate valve, a fifth gate valve, a sixth gate valve, a plurality of seventh gate valves, a first tee joint, a second tee joint, a plurality of tap joints;

the sixth pipeline comprises a sixth first pipeline, a sixth second pipeline and a sixth third pipeline, the sixth first pipeline connects the public end of the sixth option valve with the hydraulic power device for providing the reagent, the sixth second pipeline is connected between one branch port of the sixth option valve and the first port of the first three-way joint, and the sixth third pipeline is connected with the other branch port of the sixth option valve;

the fifth pipeline comprises a fifth first pipeline, a fifth second pipeline and a fifth third pipeline, the fifth first pipeline connects the public end of the fifth selective valve with the diluent tank, the fifth second pipeline is connected between one branch port of the fifth selective valve and the second port of the first three-way joint, the fifth third pipeline is connected between the other branch port of the fifth selective valve and the first port of the second three-way joint, and the second port of the second three-way joint is connected with the liquid power device for absorbing, spitting and mixing;

the fourth pipeline includes first pipeline of fourth, fourth two pipelines and fourth three pipeline, first pipeline of fourth is through a plurality of the tap will the common port of fourth select valve is with a plurality of the mixing pond is connected, and is a plurality of seventh select valve is connected the mixing pond with on the pipeline between the tap, fourth two tube coupling in one of fourth select valve divide the port with between first three way connection's the third port, fourth three tube coupling in another of fourth select valve divide the port with between second three way connection's the third port.

13. The specific protein detection module according to claim 4, further comprising a fourth gate valve, a fifth gate valve, a sixth gate valve, a plurality of seventh gate valves, a first three-way joint, a plurality of second three-way joints, a plurality of shunt joints;

the fifth pipeline connects the diluent tank with a second port of the first three-way joint, and the fifth gate valve is connected to the fifth pipeline;

a plurality of three ports of second three way connection are connected respectively the mixing pond, be used for inhaling and spit the mixing the hydrodynamic device the one end of fourth gate valve, the other end of fourth gate valve is through a plurality of shunt connection with first three way connection's third port is connected.

14. The specific protein detection module according to claim 4, further comprising a fourth gate valve, a fifth gate valve, a sixth gate valve, a plurality of seventh gate valves, a first three-way joint, and a shunt joint;

the fifth pipeline comprises a fifth first pipeline, a fifth second pipeline and a fifth third pipeline, the fifth pipeline connects the public end of the fifth selective valve with the liquid power device for absorbing, spitting and uniformly mixing, the fifth second pipeline is connected between one branch port of the fifth selective valve and the second port of the first three-way joint, the fifth third pipeline is connected between the other branch port of the fifth selective valve and one branch port of the fourth selective valve, and the third port of the first three-way joint is connected with the other branch port of the fourth selective valve;

the public end of the fourth selective valve is connected with the mixing pool through a plurality of shunt joints, and the seventh selective valve is connected on a pipeline between the mixing pool and the shunt joints.

15. The specific protein detection module according to claim 1, further comprising a common pipeline connected to the plurality of fourth pipelines through a shunt joint, wherein a capacity expansion pipeline is arranged between the common pipeline and the detection assembly, and the capacity expansion pipeline increases a buffer volume in a manner of increasing a pipeline diameter or increasing a pipeline length so as to prevent the blending reagent from exceeding the shunt joint when the blending reagent is sucked, spitted and blended.

16. A blood testing apparatus comprising a control analysis module, a collection and distribution module, a blood routine measurement module and a specific protein detection module according to any one of claims 1-15 electrically connected;

the collection and distribution module is used for collecting blood samples and distributing the blood samples to the blood routine measurement module and the specific protein detection module;

the specific protein detection module is used for testing specific protein parameters of the blood sample;

the blood routine measurement module is used for testing blood routine parameters of the blood sample.

17. The blood testing apparatus of claim 16, wherein the specific protein parameters comprise CRP, SAA, PCT, D-Dimer, TRF, Hs-CRP, ADPN.