CN109985679A - A kind of SPR detector multichannel micro-fluidic chip grasping system - Google Patents
A kind of SPR detector multichannel micro-fluidic chip grasping system Download PDFInfo
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- CN109985679A CN109985679A CN201910335974.8A CN201910335974A CN109985679A CN 109985679 A CN109985679 A CN 109985679A CN 201910335974 A CN201910335974 A CN 201910335974A CN 109985679 A CN109985679 A CN 109985679A
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- adjusting bracket
- slot
- chip
- transition interface
- fluidic chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/55—Specular reflectivity
- G01N21/552—Attenuated total reflection
- G01N21/553—Attenuated total reflection and using surface plasmons
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measurement Of Levels Of Liquids Or Fluent Solid Materials (AREA)
Abstract
The invention discloses a kind of SPR detector multichannel micro-fluidic chip grasping systems, including adjustable chip set and lid pressing plate；Adjustable chip set includes upper and lower horizontally disposed two threaded rods, left adjusting bracket and right adjusting bracket, and the left side of the right side of left adjusting bracket and right adjusting bracket offers chip placing slot；Lid pressing plate includes integrally formed interconnecting piece and transition interface mounting portion, and being horizontally disposed with from top to bottom on transition interface mounting portion has several groups transition interface to install through slot, and sliding is provided with several transition interfaces in transition interface installation through slot；The lower end of transition interface is provided with sealing ring.
Description
Technical field
The present invention relates to a kind of chip grasping systems more particularly to a kind of SPR detector to be pressed from both sides with multichannel micro-fluidic chip
Hold system.
Background technique
Surface plasma resonance (SPR) is a kind of physical optics phenomenon.In two kinds of different refractivity (refractive
Index on transparent medium interface) (such as glass and water), it is situated between when Ray Of Light is incident on low-refraction from high refractive index medium
Matter, light will occur to reflect and reflect.When incidence angle increases to a certain particular value, refraction angle be equal to 90 °, this time along with
The tangent direction in interface is projected, and incidence angle at this time is known as critical angle.If incidence angle is more than critical angle, incident ray will not
Another transfer can be entered, and be all reflected back toward in incident medium, experiences total internal reflection.
In fact, although whole incident light is reflected, a kind of electromagnetic field being evanescent wave can pass through contacting permeation to low folding
It penetrates in rate medium, energy is exponentially decayed.If plating one layer of metallic film (general gold-plated film or silverskin), metal in interface
The free electron of film surface is excited by incident light and generates charge oscillation, and then forms surface plasma.Adjustment light enters
When firing angle or wavelength are to a certain appropriate value, surface plasma is equal with the frequency of evanescent wave and wave number, and energy just occurs for the two
Coupling forms surface plasma resonance.The total reflection condition of interface will be destroyed when resonance, and incident laser energy is transferred to table
In surface plasma wave, sharply decline in the air so as to cause intensity of reflected light, decaying total reflection phenomenon is presented.Wherein make anti-
It penetrates the incident light angle that light completely disappears and is known as resonance angle (SPR angle).Resonance angle can be with the medium of metal film surfaces
The change of refractive index and change, and refractive index variation it is directly proportional to the quality for the molecule for being incorporated in metal surface.Therefore pass through
Analyze resonance angle, so that it may obtain the information of intermolecular interaction.
SPR sensorgram technology, which has without label, to surface characteristic and substance, changes sensitivity, in real time, quickly and easily realizes
The features such as automation.The technology has been widely used in life science, clinical diagnosis, drug screening, food safety, environmental monitoring
Equal fields, test object includes albumen, nucleic acid, hormone, toxin, pesticide, cell, microorganism etc..
Micro-fluidic chip is the detection chip used in SPR detection device, currently, micro-fluidic chip is being installed and used
When, it needs for micro-fluidic chip and pipeline to be attached by modes such as contact pin, gluings.But the above method belongs to irreversible company
It connects, micro-fluidic chip can not continue to use after dismantling with fluid conduit systems, and colloid also easily pollutes sample.
Summary of the invention
The object of the present invention is to provide a kind of SPR detector multichannel micro-fluidic chip grasping systems, convenient for micro-fluidic
Chip is installed and used, while can also carry out multichannel micro-fluidic chip normally sample injection testing.
The present invention adopts the following technical solutions:
A kind of SPR detector multichannel micro-fluidic chip grasping system, including adjustable chip set and lid pressing plate；
The adjustable chip set includes upper and lower horizontally disposed two threaded rods.Be arranged on threaded rod structure it is identical and
The left side of symmetrically arranged left adjusting bracket and right adjusting bracket, the right side of left adjusting bracket and right adjusting bracket offers core
Locking device is additionally provided on threaded rod on the outside of piece resettlement groove, left adjusting bracket and right adjusting bracket；
The lid pressing plate includes integrally formed interconnecting piece and transition interface mounting portion, the upper and lower ends of transition interface mounting portion
It is provided with interconnecting piece, the end of interconnecting piece is provided with threaded rod perforation and is set between left adjusting bracket and right adjusting bracket
Threaded rod on, on transition interface mounting portion from top to bottom be horizontally disposed with have several groups transition interface install through slot, transition interface
Sliding is provided with several transition interfaces in installation through slot；The lower end of transition interface is provided with sealing ring.
Limiting device up and down is additionally provided in the right side of the left adjusting bracket and the chip placing slot of right adjusting bracket.
The limiting device up and down includes the limit through slot up and down of the slot bottom setting up and down in each chip placing slot, on
There are two gag lever post, the diameter of two gag lever posts is greater than the width of upper and lower limiting slot, two gag lever posts for setting in lower limit through slot
The left and right sides on top offers and the gag lever post sliding groove that limit through slot matches up and down, and the upper end of two gag lever posts is located at
In chip placing slot and it is respectively and fixedly provided with limit plate, the lower end of two gag lever posts is respectively positioned on the lower section of chip placing slot, two limits
The lower part of bar has also been connected through a screw thread limit rod locking device.
The surface of the limit plate and the surface of chip placing slot are provided with flexible rubber layer.
The transition interface includes sliding shoe and the transitional connection pipe that the fast inside of sliding is arranged in, sliding shoe on both sides of the middle
It is provided with the sliding shoe sliding groove to match with transition interface installation through slot, sliding shoe center vertical is provided with screw thread installation
Hole, transitional connection pipe pass through transitional connection pipe and are threadedly coupled with sliding shoe, and the upper and lower ends of transitional connection pipe are respectively positioned on sliding shoe
The lower end of outside, transitional connection pipe is fixedly connected with sealing ring.
The threaded upper ends of the transitional connection pipe are connected with locking cap.
The left or right side of the transition interface installation through slot and extraneous conducting.
The locking device uses locking nut.
The lower end surface of the left adjusting bracket and right adjusting bracket is each provided with two supporting legs.
Clamping device is provided on two supporting legs of the lower end surface setting of the left adjusting bracket and right adjusting bracket.
The present invention is the chip for adapting to different size specification, is adapted to the chip of different length, using left adjusting bracket and
Right adjusting bracket is realized to the clamping of chip left and right directions and the support of vertical direction, and by being respectively provided in chip placing slot
Limiting device up and down realization clamp limit in the front-back direction to chip.Multiple groups transition interface installs the multiple mistakes being arranged in through slot
Cross interface, it can be ensured that in chip it is each enter liquid bath and liquid outlet groove can use and arrive corresponding transition interface, and connect by transition
The close contact of the sealing ring and chip surface of the setting of mouth lower end realizes transition interface and enters the connection of liquid bath and liquid outlet groove.It keeps away
Exempt from existing micro-fluidic chip and pipeline and carries out unidirectional type connection by modes such as contact pin, gluings, it is repeatable to continue to use, together
When effectively prevent colloid pollution caused by sample.
Detailed description of the invention
Fig. 1 is overlooking structure diagram of the invention；
Fig. 2 is side structure schematic view of the invention；
Fig. 3 is the attachment structure schematic diagram of gag lever post and limit plate in the present invention；
Fig. 4 is the structural schematic diagram of transition interface in the present invention.
Specific embodiment
The present invention is made with detailed description below in conjunction with drawings and examples:
As shown in Figures 1 to 4, SPR detector multichannel micro-fluidic chip grasping system of the present invention, including it is adjustable
Chip set and lid pressing plate；
The adjustable chip set, for realizing the positioning to chip, adjustable chip set includes horizontally disposed up and down
Two threaded rods 1.Identical structure is arranged on threaded rod 1 and symmetrically arranged left adjusting bracket 2 and right adjusting bracket 3, it is left
Adjusting bracket 2 and right adjusting bracket 3 can be moved by two threaded rods 1, and the chip of different in width is cooperated to use；Left adjusting branch
It is additionally provided with locking device 4 on the threaded rod 1 in 3 outside of frame 2 and right adjusting bracket, locking nut can be used in locking device 4, can
After left adjusting bracket 2 and right adjusting bracket 3 clamp chip in left and right directions, to left adjusting bracket 2 and right adjusting bracket 3 into
Line position sets locking, prevents the shaking of chip.The right side of left adjusting bracket 2 and the left side of right adjusting bracket 3 offer chip peace
Set slot 5, the bottom surfaces of two chip placing slots 5 can the bottom surface respectively to the left part of chip and right part be supported, guarantee chip
It is in a horizontal position in the vertical direction, the side that the side of two chip placing slots 5 can respectively to the left part of chip and right part
Face is supported, and realizes limit of the chip on horizontal left and right directions.On the other hand, due to being suitable for SPR detector multi-pass
Road micro-fluidic chip is different from general micro-fluidic chip, is provided with gold-plated glass plate below, and gold-plated glass plate needs and light
The surface contact of reflecting prism in linear system system, because using the two chip placing slots 5 in left and right respectively to chip in the application
The bottom surface of left part and right part is supported, and reserves intermediate space so as to the table of the reflecting prism in gold-plated glass plate and light system
Face contact.
In order to further be adapted to the chip of different length, realize that good clamping position is made with the chip to different size
With, in the present embodiment, be provided in the right side of left adjusting bracket 2 and the chip placing slot 5 of right adjusting bracket 3 up and down limit dress
It sets.
Upper and lower limiting device includes the through slot of limit up and down 6 of the slot bottom setting up and down in each chip placing slot 5, bound
There are two gag lever post 7, the diameter of two gag lever posts 7 is greater than the width of upper and lower limiting slot 6, two gag lever posts 7 for setting in the through slot 6 of position
The left and right sides on top offers and the gag lever post sliding groove 8 that limit through slot 6 matches up and down, the upper end of two gag lever posts 7
In chip placing slot 5 and it is respectively and fixedly provided with limit plate 8, each two limit plates 8 in left adjusting bracket 2 and right adjusting bracket 3, energy
Enough two end faces up and down to chip carry out good clamping.Position of the chip in chip placing slot 5 can also be realized simultaneously
Adjustment.The lower end of two gag lever posts 7 is respectively positioned on the lower section of chip placing slot 5, and the lower part of two gag lever posts 7 is also connected through a screw thread
There is limit rod locking device 11, convenient for carrying out position locking to the chip behind adjustment position.Limit rod locking device 11 can be used
Truncated cone-shaped thread cap.
In order to avoid chip is impaired, the surface of limit plate 8 and the surface of chip placing slot 5 are provided with flexible rubber layer
10。
Lid pressing plate enables transition interface 12 to be located at and enters liquid bath in chip for fixing and adjusting transition interface 12
Transitional function is played to realize that chip enters the connection of liquid bath and liquid outlet groove and pipeline, without adopting in position corresponding with liquid outlet groove
The connection of contact pin, gluing is carried out with traditional approach.
Lid pressing plate includes integrally formed interconnecting piece 13 and transition interface mounting portion 14, transition interface mounting portion 14 up and down
Both ends are provided with interconnecting piece 13, and the end of interconnecting piece 13 is provided with threaded rod perforation and is set in left adjusting bracket 2 and right tune
It saves on the threaded rod 1 between bracket 3, lid pressing plate realizes position adjustment by two threaded rods 1.On transition interface mounting portion 14 from
Up to lower level be provided with several groups transition interface installation through slot 15, transition interface installation through slot 15 in sliding be provided with it is several
A transition interface 12；Multiple groups transition interface install through slot 15 in multiple transition interfaces 12, it can be ensured that in chip it is each enter liquid
Slot and liquid outlet groove, which can use, arrives corresponding transition interface 12.The lower end of transition interface 12 is provided with sealing ring 16, can pass through
With the close contact of chip surface, realizes transition interface 12 and enter the connection of liquid bath and liquid outlet groove.
In the present embodiment, transition interface 12 includes sliding shoe 17 and the transitional connection pipe 18 being arranged in inside sliding shoe 17,
Sealing ring 16 is fixedly connected with the lower end of transitional connection pipe 18.17 on both sides of the middle of sliding shoe is provided with logical with transition interface installation
The sliding shoe sliding groove 19 that slot 15 matches, transitional connection pipe 18 are realized by sliding shoe 17 and are installed in through slot 15 in transition interface
The movement of left and right directions.17 center vertical of sliding shoe is provided with threaded mounting hole, and transitional connection pipe 18 passes through transitional connection pipe 18
It is threadedly coupled with sliding shoe 17, the upper and lower ends of transitional connection pipe 18 are respectively positioned on outside sliding shoe 17, when transitional connection pipe 18 moves
Move to it is corresponding enter liquid bath and liquid outlet groove above when, can be moved downward by screw thread, it is ensured that closely pressing is entering sealing ring 16
The chip surface of liquid bath and liquid outlet groove periphery.
The upper end of transitional connection pipe 18 is also threaded with truncated cone-shaped locking cap 20, further ensures that transitional connection pipe 18
Stablize position, it is ensured that the stabilization of lower pressure；The locking cap 20 of truncated cone-shaped is also convenient for effectively connecting with peripheral hardware pipeline simultaneously.Transition connects
The left or right side of mouth installation through slot 15 and extraneous conducting, use different number according to the demand of actual use convenient for staff
Transition interface 12.
In view of the present invention is stably connected with the reflecting prism in light system, left adjusting bracket 2 and right adjusting bracket 3
Lower end surface be each provided with two supporting legs 21, be provided with clamping device 22 on two supporting legs 21.Clamping device 22 can be used existing
Screw rod clamping, the modes such as spring clamping, it is ensured that with the reflecting prism shell in light system it is effective fixation.
Claims (10)
1. a kind of SPR detector multichannel micro-fluidic chip grasping system, it is characterised in that: including adjustable chip set and
Lid pressing plate；
The adjustable chip set includes upper and lower horizontally disposed two threaded rods.Be arranged on threaded rod structure it is identical and
The left side of symmetrically arranged left adjusting bracket and right adjusting bracket, the right side of left adjusting bracket and right adjusting bracket offers core
Locking device is additionally provided on threaded rod on the outside of piece resettlement groove, left adjusting bracket and right adjusting bracket；
The lid pressing plate includes integrally formed interconnecting piece and transition interface mounting portion, the upper and lower ends of transition interface mounting portion
It is provided with interconnecting piece, the end of interconnecting piece is provided with threaded rod perforation and is set between left adjusting bracket and right adjusting bracket
Threaded rod on, on transition interface mounting portion from top to bottom be horizontally disposed with have several groups transition interface install through slot, transition interface
Sliding is provided with several transition interfaces in installation through slot；The lower end of transition interface is provided with sealing ring.
2. SPR detector multichannel micro-fluidic chip grasping system according to claim 1, it is characterised in that: described
The right side of left adjusting bracket and the chip placing slot of right adjusting bracket in be additionally provided with up and down limiting device.
3. SPR detector multichannel micro-fluidic chip grasping system according to claim 2, it is characterised in that: described
Limiting device up and down include the slot bottom setting up and down in each chip placing slot limit through slot up and down, up and down limit through slot in
There are two gag lever post, the diameter of two gag lever posts is greater than the width of upper and lower limiting slot, the left and right two on two gag lever post top for setting
Side offers and the gag lever post sliding groove that limit through slot matches up and down, and the upper end of two gag lever posts is located in chip placing slot
And it is respectively and fixedly provided with limit plate, the lower end of two gag lever posts is respectively positioned on the lower section of chip placing slot, and the lower part of two gag lever posts is also logical
It crosses and is threaded with limit rod locking device.
4. SPR detector multichannel micro-fluidic chip grasping system according to claim 3, it is characterised in that: described
The surface of limit plate and the surface of chip placing slot be provided with flexible rubber layer.
5. SPR detector multichannel micro-fluidic chip grasping system according to claim 1, it is characterised in that: described
Transition interface include sliding shoe and the fast internal transitional connection pipe of sliding be set, sliding shoe on both sides of the middle is provided with and mistake
The sliding shoe sliding groove that interface installation through slot matches is crossed, sliding shoe center vertical is provided with threaded mounting hole, transitional connection pipe
It is threadedly coupled across transitional connection pipe with sliding shoe, the upper and lower ends of transitional connection pipe are respectively positioned on outside sliding shoe, transition connection
The lower end of pipe is fixedly connected with sealing ring.
6. SPR detector multichannel micro-fluidic chip grasping system according to claim 5, it is characterised in that: described
The threaded upper ends of transitional connection pipe be connected with locking cap.
7. SPR detector multichannel micro-fluidic chip grasping system according to claim 6, it is characterised in that: described
Transition interface installation through slot left or right side and extraneous be connected.
8. SPR detector multichannel micro-fluidic chip grasping system according to claim 1, it is characterised in that: described
Locking device use locking nut.
9. SPR detector multichannel micro-fluidic chip grasping system according to claim 1, it is characterised in that: described
Left adjusting bracket and the lower end surface of right adjusting bracket be each provided with two supporting legs.
10. SPR detector multichannel micro-fluidic chip grasping system according to claim 9, it is characterised in that: described
Left adjusting bracket and right adjusting bracket lower end surface setting two supporting legs on be provided with clamping device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910335974.8A CN109985679A (en) | 2019-04-24 | 2019-04-24 | A kind of SPR detector multichannel micro-fluidic chip grasping system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910335974.8A CN109985679A (en) | 2019-04-24 | 2019-04-24 | A kind of SPR detector multichannel micro-fluidic chip grasping system |
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| Publication Number | Publication Date |
|---|---|
| CN109985679A true CN109985679A (en) | 2019-07-09 |
Family
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|---|---|---|---|
| CN201910335974.8A Withdrawn CN109985679A (en) | 2019-04-24 | 2019-04-24 | A kind of SPR detector multichannel micro-fluidic chip grasping system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113019490A (en) * | 2021-03-05 | 2021-06-25 | 苏州工业职业技术学院 | Micro-current control chip fixing clamp capable of being micro-adjusted |
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Application publication date: 20190709 |
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