CN102466581B - A kind of device - Google Patents
A kind of device Download PDFInfo
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- CN102466581B CN102466581B CN201010538076.1A CN201010538076A CN102466581B CN 102466581 B CN102466581 B CN 102466581B CN 201010538076 A CN201010538076 A CN 201010538076A CN 102466581 B CN102466581 B CN 102466581B
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Abstract
The invention provides a kind of device, comprise sample chamber, control the device of fluid circulation and increase atmospheric device; Wherein, the device that sample chamber and control fluid circulate is connected; Increase atmospheric device for increasing the air pressure in sample chamber.Because the pressure ratio ambient pressure in sample chamber is large, the fluid in chamber is made to flow out to outside chamber smoothly; When special sample size is less, because the air pressure in sample chamber exists, ensure flow and the flow velocity of fluid, and then ensure that next step detects the sample size needed, guarantee the accuracy detected; In addition, the air pressure of increase can allow sample flow out outside sample chamber fast, provides the efficiency of process sample, has saved the time completing whole detection generally.The present invention also can with gathering-device, pick-up unit is bonded together, formed sample collection, process and detect one.This device is light and handy, conveniently transports or carries, and is particularly suitable for family and uses.
Description
Technical field
The present invention relates to the device of process sample, especially sample needs pretreated device before detection;
And use the method for this device process sample.
Background technology
The various sample collection of Clinical practice or family expenses and the proving installation of extraction, have in document and described by having.These proving installations can utilize the one in various collection kit to obtain sample and to be transferred to storage.Sample can be extracted from gathering-device, then sample is sent to testing element whether to determine the existence of certain material, such as, analyze quality testing and survey.These devices may be used for target classification, comprise detection of drugs and biologic artifact, such as glucose or hormone, antibody or cause of disease.Usually, need the sample existence form of detection various, as solid, semi-solid, liquid or gas etc., they cannot detect under original state, or testing result is undesirable, usually need the physics or the chemical characteristic that are changed them by pre-service, such as, help dilution by buffer solution or cushion these samples, then detect then.
Summary of the invention
The problem to be solved in the present invention is to provide a kind of device, when using this device, can increase and process flow that is rear or storage fluid sample outflow sample chamber in a device, flow when particularly sample size is less, and the time of bleeder; Ensure the accuracy of next step detection and improve the efficiency of whole detection, shortening the time.This device can to the process before pattern detection or storing fluid sample.
On the one hand, the invention provides a kind of device, comprising: for collecting, processing or the sample chamber of storing sample; Control the device of fluid circulation; With the device increasing sample chamber internal gas pressure; Wherein, the device that sample chamber and control fluid circulate is connected.In a preferred mode, the air pressure increased in sample chamber forces the fluid being positioned at sample chamber to be flowed out to outside sample chamber by the device controlling fluid circulation.This sample chamber both can be used for collecting fluid sample, also can fluid sample process, and can also be used to collect and store fluid sample, or the set of any two functions of three functions.These functions are described later in detail below.
In another preferred mode, the device controlling fluid circulation has primary importance and the second place, when it is in primary importance time, fluid in sample chamber does not flow out by this fluid control device, in time being in the second place, the fluid in sample chamber can be flowed out to outside sample chamber by this fluid control device.Preferred, when the device controlling fluid circulation is in primary importance time, the air pressure in sample chamber increases; When the device controlling fluid circulation is in the second place time, the air pressure of increase forces part sample to flow out to outside sample chamber.
In another preferred mode, when sample enters after in sample chamber, increase atmospheric device and make pressure increase in sample chamber, after opening the device controlling fluid circulation, the air pressure of increase forces part sample to flow to outside sample chamber.
In some embodiments, sample chamber comprises the first and second openings, and wherein the first opening is used for receiving sample; The device that second opening circulates with control fluid is connected.More excellent, sample chamber first opening and the atmospheric means for engaging of increasing, be used for increasing the air pressure in sample chamber.
In another preferred mode, the device controlling fluid circulation has to be allowed sample chamber and the external world be in seal or non-tight two states.Sample enters in sample chamber; Increase atmospheric device to increase the air pressure in sample chamber; When after completing sample process or when needing to detect, the device controlling fluid circulation makes sample chamber be in communication with the outside (non-tight state), now, because the pressure ratio external atmospheric pressure in sample chamber is large, the fluid in chamber is impelled automatically to flow out to outside chamber.Even if when sample size is less, because of the air pressure in sample chamber, sample also can guaranteed flow and flow velocity when flowing out, and also can ensure to allow most sample be rejected to outside sample chamber because gas pressure forces simultaneously.The present invention, by the device of control flow check through-current capacity and the cooperation increasing atmospheric device, makes sample chamber be sealed and air pressure in chamber increases, thus when sample chamber is in communication with the outside, the fluid in chamber flows to outside sample chamber automatically.Like this, the flow that fluid sample flows out and speed are that the flow that flows out under normal barometric pressure and speed are large than sample chamber, further ensure the quantity detecting sample and need, guarantee the accuracy detected, shorten the time of whole detection simultaneously, improve detection efficiency.
" controlling the device of fluid circulation " of the present invention has the effect whether control sample chamber and sample chamber inner fluid and the external world are communicated with.This device can have various ways to realize such effect.Such as can for the baffle plate that can extract, it is positioned at the Single port place of sample chamber, sample chamber and interior fluid thereof can be in communication with the outside by extracting baffle plate out.Or the film that can puncture, one end port of its sealing sample chamber, when needs circulate, punctures this film, and sample fluid is flowed out by this film punctured.In one embodiment, the device of this control fluid circulation can be the valve be connected with sample chamber, controls sample chamber and whether the external world circulates by the opening and closing of valve.In a concrete embodiment, valve has primary importance and the second place in valve cavity, and when valve is positioned at primary importance, namely during valve closing, sample chamber is by valve seal; When valve is positioned at the second place, namely during valve opening, sample chamber is communicated with valve, and the fluid sample in sample chamber is discharged.More excellent, when valve is positioned at primary importance, increases atmospheric device and the air pressure in sample chamber is raised; When valve is positioned at the second place, the air pressure of rising makes the fluid sample in sample chamber discharge outside sample chamber.In one preferred embodiment, sample chamber comprises both ends open, and the device controlling fluid circulation can seal or open the opening of wherein one end, and other end opening is used for receiving fluid sample.Such as, the first opening accepts sample, the second opening connects valve and valve cavity, controls the outflow of the fluid sample in sample chamber.
In a preferred mode, the opening of sample chamber one end and a valve cavity communicate, and this valve cavity is used for collecting one valve, and valve exterior surface and valve cavity inwall are line contact sealing.Bossed lines on the outside surface of i.e. valve, these lines and valve cavity inwall form linear contact lay.Can certainly be, bossed lines on the outside surface of valve cavity, the outer surface wall of these lines and valve forms linear contact lay.Like this, the sealing property between valve and valve cavity is better, ensure that the sealing of sample chamber, also ensure that the sample in sample chamber is not leaked simultaneously, especially, when the air pressure in sample chamber increases time, the fluid sample in sample chamber also can not leak out.Better can ensure the controllability to the fluid in sample chamber like this.The present invention because there is certain air pressure in sample chamber, so sealing is very important; Meanwhile, when sample size is less, in order to ensure Detection results and do not make sample leak accurately, sealing is also extremely important.
In addition, " increasing atmospheric device " of the present invention has the function that the air pressure in the sample chamber of sealing is increased.Specifically, a piston structure can be used to realize this function, or, the air pressure in chamber can be increased in the mode of the chemical reaction generation gas of sample intracavity liquid.One preferred embodiment in, use seal element sealing sample chamber also to form hermetic seal with the madial wall of sample chamber, and by the motion of seal element in sample chamber, sample chamber internal gas pressure increased.In preferred mode, seal one end open sample chamber receiving fluid sample with seal element, and the device controlling fluid circulation can seal or open the opening of the other end in sample chamber.Wherein, when other end opening is sealed time, allow the air pressure in sample chamber increase, when the other end is opened time, the air pressure of increase forces segment fluid flow to flow out to outside sample chamber.
In embodiments all above, this device can also comprise test chamber and testing element, and the device that test chamber circulates with control fluid is connected, and testing element is positioned at test chamber.In a preferred mode, test chamber is communicated with valve cavity, and whether the fluid in Valve controlling sample chamber flows in test chamber.In preferred mode, the air pressure increased in sample chamber forces segment fluid flow to flow out to outer rear directly the entering in test chamber of sample chamber and contacts with testing element.In another mode, the chamber of valve cavity also with one stored samples is connected; The air pressure increased in sample chamber can also force segment fluid flow to flow out to sample chamber and directly enter in sample storage chamber outward.Certainly, forced the fluid flowed out to outside sample chamber can directly enter test chamber and storage chamber by the air pressure increased in sample chamber simultaneously.
In other embodiments, before sample enters sample chamber, the reagent for the treatment of sample can be deposited in advance in sample chamber.This reagent can be the solution investing the sample in sampler for diluted sample or wash-out; Certainly, these reagent also can enter after in sample chamber at sample and adding and fully mixing with sample.These reagent can be reagent or the solution of conventional buffer solution or other purposes.
Certainly, after collection sample to sample chamber, sample can be allowed to be stored in sample chamber, when waiting needs to detect or need segment fluid flow sample, open fluid control device and flow out to outside sample chamber by fluid sample.Before opening fluid control device, can first allow the air pressure in sample chamber increase, the air pressure of increase forces segment fluid flow automatically to flow to and directly to test outside sample chamber or to chemically examine.
In other preferred modes, this device can also comprise a lid, and lid is positioned in sample chamber, is used for receiving the opening of sample for sealing sample cavity one end.The juncture of lid and sample chamber is various, as snap engagement, and forced joint etc.In an embodiment, there is inside lid screw-in screw thread, and sample chamber has the reception screw thread of supporting joint with it.In another embodiment, before use, have the reagent of process sample, such as buffer solution is stored in sample chamber in advance, and lid is positioned on sample receptive cavity, and the buffer in guarantee chamber and the sample collected subsequently are not leaked.
In a more excellent embodiment, lid with increase atmospheric device with the use of, when lid lid is to sample chamber opening time, the air pressure in sample chamber can be increased simultaneously.Increasing atmospheric device can be seal element, and seal element carrys out individualism to lid, and can arrange a jut on the inwall of lid.When sample enters in sample chamber, now, cover lid, protuberance contacts in lid is to seal element, and along with lid engages with the opening of sample chamber, the projection of cover inside promotes seal element and moves bottom sample chamber, thus reach compression intracavity gas, increase the effect of chamber internal gas pressure.
In another embodiment, sealing element is attached on lid.Seal element can be connected with lid, and equally, when lid engages with sample chamber opening, seal element also seals sample chamber opening and the air compressed in it thereupon.In a more excellent embodiment, seal element is positioned on the inwall of cap top, and now cap top has certain thickness, to ensure, when pressurized air, can reach certain force of compression.
Sample chamber of the present invention, in order to ensure fully mixing of sample and buffer solution, structurally can also carry out particular design.As, extruding baffle plate is set in chamber, or this sample chamber uses soft extruded material to make etc.In an embodiment, be funnel-shaped structure in the middle part of this sample chamber, namely this sample chamber top is larger than lower diameter, and when the sample collection rod being adsorbed with sample is placed in after in sample chamber, the absorption piece collected on rod is positioned at the Lower Half of sample chamber.This lower chamber structure ensures that the buffer solution in sample chamber floods absorption piece enough completely, and the sample on absorption piece can fully be contacted with buffer solution, thus reaches the object of fully process sample.
The present invention also provides a kind of box simultaneously, for the treatment of sample, comprises the present invention's sample device described above, and box body and sample collection rod.Box body by sample chamber, with control fluid circulation means for engaging together with.Further, the box of this process sample also comprises test chamber and testing element, and test chamber and testing element are positioned at box body too.When in use, sample collection rod is for collecting the sample of testee, and this sample can be solid, semi-solid, liquid or gas sample.Sample collection rod after sampling is put into sample chamber, then buffer solution is added, re-use and increase atmospheric device to increase sample chamber internal gas pressure, such as, seal element is put into sample chamber, sealing air in compression chamber or the lid that directly covers with seal element.Certainly, buffer solution also can add in advance in sample chamber before this device uses.Then, open the device controlling fluid circulation, as valve, sample chamber is in communication with the outside.In an embodiment, sample chamber is connected by valve cavity with test chamber, and the sample after process flows out sample chamber because of the gas pressure in sample chamber and enters into test chamber, contacts, complete detection with testing element.
In some embodiments, the height of sample chamber is greater than the length of sample collection rod.Whole sample collection rod can be held.And sample chamber lower chamber height is more than or equal to the absorber element of sample collection rod, ensure that absorber element is arranged in lower chamber completely.
In addition, the present invention also provides a kind of and processes sample and the method that sample is flowed out, and it comprises: the reagent of sample and/or process sample is added sample chamber; Increase the air pressure in sample chamber; Sample chamber is in communication with the outside; The air pressure increased forces sample and/or process sample reagent, or the mixed liquor of the two flows out to outside sample chamber.
In a preferred mode, whether the fluid controlled in sample chamber by the device controlling fluid is flow to outside sample chamber.In another preferred mode, sample chamber is both ends open, controls the device of fluid and is connected with an opening, and increases atmospheric device and increase air pressure by another opening.
In some embodiments, control sample chamber by the device controlling fluid circulation and be communicated with extraneous, such as, the baffle plate that can extract, the film that can puncture.In an embodiment, by Valve controlling sample chamber with being communicated with of the external world.
In other embodiments, by increasing atmospheric device, sample chamber internal gas pressure is increased, such as: piston apparatus, or, gas in the cavity of sealing is made to increase thus increase air pressure etc. by the aqueous chemical reaction in sample chamber, one preferred embodiment in, seal sample chamber and gas in compression chamber by seal element, thus increase air pressure; This increase air pressure makes the outflow of sample mixed liquor.
In some embodiments, Buffer solution can enter before or after sample chamber at sample and join in sample chamber.
In some embodiments, sample can be collected with the cotton swab of sample collection rod, then, the sample collection rod with sample be put into sample chamber.
In other embodiments, make the fluid contact testing element flowed out in sample chamber, detect sample or its potpourri.Beneficial effect
The present invention is large because of the pressure ratio ambient pressure in sample chamber, makes the fluid in chamber to flow out to outside chamber smoothly.When special sample size is less, because of the air pressure in sample chamber, also can guaranteed flow and flow velocity.And then ensure that next step detects the sample size needed, guarantee the accuracy detected.Meanwhile, the air pressure of increase can allow sample flow out outside sample chamber fast, provides the efficiency of process sample, has saved the time completing whole detection generally.The present invention also can with gathering-device, pick-up unit is bonded together, formed sample collection, process and detect one.This device is light and handy, conveniently transports or carries, and is particularly suitable for family and uses.
Accompanying drawing explanation
Fig. 1 is the schematic perspective view of apparatus of the present invention
Fig. 2 is valve mechanism schematic diagram in device
Fig. 3 is another angle valve mechanism schematic diagram
Fig. 4 is profile valve figure
Fig. 5 is the sectional view of valve other direction
Fig. 6 is this device sample chamber cap structure exploded view
Fig. 7 is device sample chamber cap structure decomposing section
Fig. 8 to Figure 10 is the process schematic engaged with sample chamber with the lid of seal element
Figure 11 is the schematic perspective view (valve is in closed condition, the primary importance of valve in valve cavity) of the box of the present invention one process sample
Figure 12 is that box of the present invention is in the schematic diagram (second place of valve in valve cavity) of opening at valve
Figure 13 is the STRUCTURE DECOMPOSITION figure of box of the present invention
Figure 14 to Figure 17 is box operating process schematic diagram of the present invention
The explanation of accompanying drawing mark
Sample chamber 100; Sample chamber accent (the first opening) 101; At the bottom of sample chamber chamber 102, sample chamber upper cavity 103; Sample chamber lower chamber 104; Sample chamber madial wall 106; Bottom, sample chamber chamber opening (the second opening) 107; Sample chamber first opening part raised structures 109; Sample chamber window 108; Valve 200; Valve switch 201; Valve exterior surface lines 203a, 203b, 203c, 203d, 203e; Valve cavity 206; Valve cavity and test chamber passage 205; Valve passage 800; Valve hole 801,802; Valve exterior surface 207,2071,2072,2073; Valve cavity inwall 208; Test sample chamber 300; Testing element 301; Testing element uptake zone 3011; Filtration unit 302; Test chamber window 307; Seal element 400; Lid 500; Cap top 501; Lid 502; Box body 600; Box body upper cover 601; Box body lower cover 602; Sample collection rod 700; Collect excellent hand part 701; Absorption piece 702;
Below the technical term that structure or these of liquid-sample collection device use is described further.
sample
" sample " that the present invention refers to refers to any material whether needs chemical examination exists and (or) analyze analyte concentration, or need to determine whether one or more samples exist and (or) the material of analyte of quantity, or need the material carrying out qualitative evaluation.Sample can be fluid sample, such as liquid sample.Liquid sample comprises body fluid, such as blood, serum, blood plasma, saliva, urine, tears, seminal fluid and marrow; Liquid sample also can be water sample, such as seawater, river, river etc., or from domestic water, municipal water use or service water resource, runoff water or sewage; Sample can be food sample, such as milk and wine.Mucus, semisolid or solid sample can be used for making liquid, eluate, suspending liquid or leachate equal samples.Such as, throat or genitals sample may be dipped in liquid and make sample.Sample can comprise the potpourri of liquid, solid and gas or any relevant potpourri, the cell suspending liquid in such as dilution or solution.Sample comprises biological substance, such as cell, microorganism, organelle and biochemical complexes.Liquid sample can be produced from solid, semisolid or high-viscosity materials such as such as soil, ight soil, tissue, organ, biological fluid or other occurring in nature non-liquid samples.Such as, these solids or semi-solid samples can mix by the solution suitable with dilution one class.Sample can be macerated, freezing and thaw, or other extracting method form liquid sample.Remaining particulate material can use the method that filtration or precipitation etc. are traditional to remove.
testing element
" testing element " is any element performing test.In one embodiment, testing element is test-strips.This test-strips can comprise thereon for the material pair with specific binding of immunoassay.Test-strips can be a kind of change by color upon completion of the assays or the test chemical bar of other signal intensity judged results.Be suitable for the sample that the present invention carries out detecting and include but not limited to body fluid, the sample be separated from biological tissue or body fluid.Such as, sample can be saliva, blood, serum, blood plasma, urine, excreta, spinal fluid, vaginal secretion, mucus and tissue.Testing element is not limited to one, can be that two or more testing element is arranged in pick-up unit simultaneously, respectively different component in test sample book.
sample collection rod
The present invention also provides sample collection rod 700.In one embodiment, sample collection rod 700 has an absorber element 702 and is affectedly bashful portion 701.Other sponge of medical grade that absorber element 702 is commonly used by this area usually or plastic foam material are made.But many other materials also can be made into absorber element, such as cotton or paper, or other any materials with water absorbing properties.Applicator can soaked containing stimulating in the salivary solution of tester in advance.Thus make more easily to collect saliva when tester's mouth put into by gatherer.Be affectedly bashful portion's normally rigidity, be conducive to the operation to absorber element.The material that the portion of being affectedly bashful can be commonly used by this area is made, such as plastics, timber, metal or cardboard.In a preferred mode, sample collection rod is European patent, number EP1893740B1, the flocking cotton swab described in EP1608268B1 (flocked swab).
Embodiment
In the following detailed description, accompanying drawing and corresponding explanatory note are only illustrate that the present invention can the mode of actable specific concrete scheme by way of example.We do not get rid of the present invention can also without prejudice to any other embodiment within the scope of the claims in the present invention.
The invention provides a kind of device processing sample, the device that this device is circulated by sample chamber 100 and control fluid and the device increasing sample chamber internal gas pressure are formed.Wherein, sample chamber 100 is connected with the device controlling fluid circulation.
In some embodiments, the device controlling fluid circulation is valve 200 structure.The valve mechanism that this valve and industry generally use is identical with function, namely when closing, connected parts is not communicated with, and after opening, is then communicated with between coupled all or part of parts.Valve wide variety, all can be used for device of the present invention, as: revolving valve, stopcock, ball valve, needle-valve, reverses valve or piston valve, gate valve, reversal valve etc.
In a specific embodiment, as Fig. 1 and in conjunction with shown in Figure 13, sample chamber 100 by the accent 101 of one end, at the bottom of chamber 102 and chamber sidewall formed, accent 101 be used for receive sample.Valve cavity 206 102 to be connected with at the bottom of chamber, has an opening 107 to communicate with valve cavity at the bottom of chamber.Valve 200 is arranged in valve cavity 206, and valve 200 has opening and closing position, is controlled by the switch 201 on valve.When valve 200 is in closed condition, when namely valve 200 is positioned at the primary importance of valve cavity 206, as shown in Figure 14 to Figure 16, then the opening 107 at the bottom of sample chamber chamber is sealed by valve 200, is not communicated with the external world; After valve 200 is opened, when namely valve 200 is positioned at the second place of valve cavity 206, as shown in figure 17, sample chamber 100 is circulated with extraneous formation by the valve 200 opened.Concretely, valve comprises a valve passage 800, after valve 200 is opened, the fluid in sample chamber enters into valve passage 800 by opening 107 and is discharged to outside sample chamber 100.
In a more excellent embodiment, in order to ensure the sealing effectiveness of valve 200, valve 200 with at the bottom of valve cavity 206 sample chamber chamber 102 inwall be line contact sealing.In some more specifically mode, the outside surface 207 that the surface of contact place of valve 200 contacts is provided with linear protrusion 203, as shown in Figures 2 and 3, Fig. 2 is the schematic diagram in valve 200 1 directions, and Fig. 3 is the schematic diagram Fig. 2 valve location rotation about 180 °.As Fig. 2, linear protrusion has 5, wherein has 2 loop wire 203d and 203e along outside surface circumference as shown in Figure 2, with 3 lines 203a that loop wire is perpendicular, and 203b, and 203c (as shown in Figure 3).Valve 200 is contacted with the inwall 208 of valve cavity 206 by these 5 wire projections, is formed and linearly contacts.Like this, the direct contact area formed that contacts with the inside surface of valve cavity 206 of the outside surface of its contact area rate valve 200 significantly reduces, and therefore, the possibility that fluid is leaked by surface of contact significantly reduces; In addition owing to being linear contact, face and face rub and become line and face must rub, and friction force reduces a lot, thus need to allow valve rotate power used and also reduce, and the control of valve is become and more easily operates.
In addition, valve 200 outside surface is divided into 3 parts by these 5 linear protrusion, is face, A face 2071, B 2072 and C face 2073 respectively.B face 2072 and C face 2073 there are respectively aperture B hole 802 and hole, C hole 801, B 802 and C hole 801 are connected in valve 200 inside and form valve passage 800.In use, because A face 2071 atresia is connected, therefore, fluid can not enter into this region and remain in this region, and therefore, effectively can ensure fluid outflow as much as possible, especially when sample size is less, effect is more obvious.
When in use, when valve 200 is positioned at primary importance time, the one end open 107 of sample chamber is by valve seal, when valve 200 is positioned at the second place time, the one end open 107 of sample chamber is opened, and is connected with valve passage, and it is external that fluid sample flows to sample chamber by valve passage 800.
The fluid sample method flow to outside sample chamber is exactly allow the air pressure in sample chamber increase, and the air pressure of increase forces fluid sample to flow out to outside sample chamber.Such as, when valve 200 is positioned at primary importance time, the air pressure in sample chamber increases, and when valve 200 is positioned at the second place time, the air pressure of increase forces fluid sample to be flowed out to outside sample chamber 100 by valve passage 800.Concrete how increasing in stressed mode embodiment below has more detailed description.
In some embodiments, increasing atmospheric device can for having the seal element 400 of sealing function, and sealing element 400, after sample enters sample chamber 100, seals sample chamber 100 from accent 101, now, seal element 400 contacts with sample chamber 100 madial wall 106 and forms hermetic seal.Then, seal element 400 102 to move and air in compression chamber at the bottom of chamber along accent 101, forms certain air pressure.
In the present invention, part sample, as solid or semi-solid samples, needs to use buffer solution dilute or neutralize, even, part sample adhesion, on sample collecting device, needs buffer solution to carry out wash-out etc., therefore, the Buffer solution of above-mentioned effect can have been added in sample chamber 100, as PH buffer solution, anti-oxidant solution, or anti-adsorbent solution and protein solution etc.These buffer solution can add before sample enters sample chamber 100, also can add after sample enters sample chamber 100.In a specific embodiment, Buffer solution is stored in sample chamber 100 in advance.In order to ensure testing result more accurately, the consumption of Buffer solution can have certain requirement, and these needs are determined according to sample properties and testing conditions.Generally, Buffer and sample size keep certain ratio.In one embodiment, the volume ratio of Buffer solution and sample size is 1: 1.In another more specifically embodiment, the volume of Buffer solution is 1 milliliter.
In some embodiments, this device also comprises lid 500, as shown in Fig. 1 and Figure 13.The opening 101 that lid 500 and one end of sample chamber 100 are used for receiving sample is formed and seals, and like this, is more conducive to Buffer solution and is pre-stored in sample chamber 100 interior (in time including solution).Sample chamber 100 is various with the juncture of lid 500, as plunger type, or buckle-type, or laminated type etc., as long as after the two combination, the Buffer solution in chamber can not leak.In an embodiment, lid 500 inside has screw-in screw thread, and the opening 101 of sample chamber 100 has reception screw thread supporting with it, and the two is for being threadedly engaged.
In other embodiments, seal element 400 can be combined with lid 500.In an embodiment, jut is designed with in lid 500 inside top, during use, after seal element 400 is positioned at sample chamber 100, cover lid 500, lid inner process part contact is to seal element 400, when lid 500 engages with sample chamber 100, along with the motion of lid 500, jut promotes seal element 400 and 102 to move at the bottom of sample chamber chamber, thus reaches the effect of compression chamber air.In another embodiment, seal element 400 is connected with lid 500, and e.g., the two is connected by thin rod, when lid 500 seals sample chamber 100, and the air while of seal element 400 in compression chamber 100.
In a preferred embodiment, seal element 400 is connected with cap top 501 is supporting, and its STRUCTURE DECOMPOSITION as shown in Figure 6 and Figure 7.Lid is made up of top 501 and lid 502.After being surrounded by seal element 400 inside cap top 501, then be combined with lid 502, form an entirety.This cap top 501 has certain height, to ensure after lid 500 covers sample chamber 100, can compress a certain amount of air, thus obtain certain air pressure.
Fig. 8 to Figure 10 shows in the process that this lid 500 engages with sample chamber 100 and increases atmospheric process.As shown in Figure 8, lid 500 engages front state with sample chamber 100, after seal element 400 engages with cap top 501, there is certain gap with lid madial wall, and this gap is for engaging the accent 101 of sample chamber.Fig. 9 is the sectional view of lid when covering sample chamber, and now, sample chamber 100 is covered by lid 500 and screw-in part downwards, and now, lid is positioned at sample chamber A position, and seal element 400 contacts with accent 101 and seals up the opening 101 of sample chamber 100 simultaneously.Figure 10 is the sectional view after lid is combined completely with sample chamber, and lid 500 is positioned at sample chamber B position.Now, seal element 400 is by air compressing in sample chamber 100, and in sample chamber, A position is compressed to the gas of B position, forms certain gaseous tension.
Sample processing apparatus provided by the invention can also comprise test sample chamber 300, and test chamber 300 is connected with sample chamber 100 by the device controlling fluid circulation, and in an embodiment, valve cavity 206 communicates with test chamber 300.Testing element 301 is comprised in test chamber 300.In one embodiment, testing element 301 is test strips.Testing element 301 in test chamber can be one or more.
In an embodiment, this valve 200 is " two-way " structure, and one end of valve passage 800 to be led at the bottom of sample chamber chamber 102 and can be communicated with opening 107, and the opening of the other end communicates with passage 205 and leads in test sample chamber 300.In a mode, after sample is positioned at sample chamber 100, now valve is in primary importance, and at the bottom of chamber, the opening 107 of 102 is sealed by valve 200; After sample chamber internal gas pressure is increased, open the switch 201 be positioned on valve, such as, switch on changeover valve, now, B hole 802 on valve and sample chamber 100 are connected by opening 107 and communicate, as shown in Figure 5, C hole 801 is connected with test chamber 300, as shown in Figure 4, then sample chamber 100 is communicated with test chamber 300 with valve passage 800 by the hole, B hole 802, C 801 on valve 200, and the sample in chamber and Buffer mixed liquor are forced by the air pressure that the passage that this is communicated with is increased and flow to test chamber 300, contact with testing element 301, complete detection.
In another embodiment, this valve can for three links: link of trade, travel and post structure, and one end to lead at the bottom of sample chamber chamber 102, and the other end leads to test sample chamber 300, with a sample, last one end confirms that chamber or storage chamber are connected.
After sample is collected in sample chamber, the direction of valve open has two, and an opening direction ensures that sample chamber 100 is connected with test chamber 300, and the sample opening 107 of 102 and passage 800 of valve 200 at the bottom of sample chamber flow into test chamber 300, contact with testing element 301, complete detection; Another opening direction ensure that sample chamber 100 and confirms being communicated with of chamber, and the fluid in sample chamber 100 can be allowed to flow into for confirming in the storage chamber that detects.Certainly, also can be, the direction that valve 200 is opened only has one, once valve open, the fluid flowed out from cavity 100 enters in valve passage 800, and valve passage is divided into again two subchannels, a subchannel leads to test chamber 300, transmits fluid and contacts with testing element 301 to test chamber; Another subchannel leads in storage chamber, transmits in fluid to storage chamber and uses with for confirming to detect.
The present invention also provides a kind of box simultaneously, for the treatment of the sample before detection, comprises sample processing apparatus and sample collection rod 700.Sample collection rod 700 is made up of hand part 701 and absorber element 702.Absorber element 702 can be made up of the material with absorption property, and as cotton swab, sponge etc., this absorber element 702 can adsorbent solid, and semi-solid, liquid is gas sample even.In a preferred embodiment, the absorber element 702 on this sample collection rod is cotton swab, and the sample of collection is saliva.
In some preferred embodiments, in order to better, sample in sample collection to sample chamber 100 and in sample chamber can better be mixed with Buffer solution, particularly when sample collection amount is less, the sample chamber 100 of special structure can be designed.Such as, in sample chamber 100, arrange dividing plate with holes, the absorption piece 702 of sample collection rod 700 can be extruded by dividing plate, fully discharges sample wherein; Or, sample chamber 100 arranges a bottleneck portion, absorption piece 702 is extruded in bottleneck, discharge sample, again or, change the material of sample chamber 100 into resiliency compressible material, after absorption piece 702 enters sample chamber 100, extruding sample chamber 100, thus discharge sample and sample is fully mixed with Buffer solution.In a preferred embodiment, sample chamber 100 upper cavity 103 is larger than lower chamber 104 diameter, and middle part is funnel-shaped structure, as shown in Figure 1.In more preferred mode, lower chamber 104 highly for can with the cotton swab 702 collecting rod contour and ensure Buffer solution in sample chamber 100 enough completely foam cross cotton swab 702, the sample on cotton swab 702 can be made so fully to contact with Buffer solution, and Buffer solution can abundant eluted sample mutually mixing with sample.
In one embodiment, seal element 400 is positioned on sample collection rod 700, after sample collection rod 700 is collected sample, when putting into sample chamber 100, the seal element 400 be located thereon enters sample chamber 100 simultaneously and is sealed, along with collection rod 700 at the bottom of sample chamber chamber 102 move time, seal element 400 102 to move thus compression samples chamber air thereupon at the bottom of chamber.Sealing element 400 can be "O"-ring, and this "O"-ring is positioned on sample collection rod 700, and certain distance is arranged at distance absorber element 702 and the excellent top of collection.
As is illustrated by figs. 11 and 12, in a specific embodiment, box body 600 is by sample chamber 100, and valve 200 and test chamber 300 and testing element 301 engage and be integrated, and Figure 13 is its STRUCTURE DECOMPOSITION figure.Box body is made up of upper cartridge body 601 and lower box body 602, and sample chamber 100 and test chamber 300 are positioned at the box body 600 that upper and lower box body is formed, and testing element 301 is positioned at test chamber 300.Box body 600 has a window 307 in the corresponding position, territory, results display area of testing element 301, and this window can be transparent, for observing testing result.In a specific embodiments, testing element 301 is test strips, and the sample absorbance district 3011 of this test strips is connected with valve 200.Specifically, as Figure 14, provide a passage 205, this passage end connecting test chamber 300, the other end connects valve cavity 206; Valve cavity 206 102 to be communicated with sample chamber by opening 107 with at the bottom of sample chamber chamber again, and whether the fluid that the valve 200 simultaneously in valve cavity 206 controls between test chamber 300 with sample chamber 100 is communicated with.Valve 200 has switchgear 201, for the opening and closing of by-pass valve control 200, namely control the circulation of sample chamber 100 and test chamber 300.In sample chamber 100, the opening 101 of one end has lid 500 to seal sample chamber, and this cap top 501 is connected with seal element 400.The box of this sample process before use, valve 200 is in closed condition, valve switch position as shown in figure 11, now, the B hole 802 of valve 200 internal valve passage 800 one end is positioned at test chamber 300 junction and communicates with test chamber, and the inwall 208 of the C hole 801 of valve passage 800 other end then valve cavity 206 engages; Valve passage 800 is not communicated with sample chamber 100 and test chamber 300, and the other end opening 107 of sample chamber 100 is sealed by the surface 207 of valve 200.For cushion to be pre-existing in the Buffer solution of eluted sample in sample chamber 100 and seal by lid 500.
When in use, sample collection rod 700 contact person under inspection is sampled, such as put in mouth and get saliva sample, then, as shown in figure 14, open the lid 500 of sample chamber 100, more as shown in figure 15, be inserted into the sample collection rod 700 of sample in sample chamber 100, make the cotton swab 702 of collection rod arrive at the bottom of sample chamber chamber 102 to ensure that Buffer solution foam crosses the cotton swab 702 on sample collection rod.Entering the state of sample chamber 100 in order to observe sample collection rod 700, on box body 600, transparent watch window 108 can be set in sample chamber lower chamber 104 corresponding position.Then, as shown in figure 16, cover lid 500 also makes lid 500 and the supporting joint of screw thread in sample chamber 100, and now, the seal element 400 being positioned at lid seals and the air in compression samples chamber 100, forms certain air pressure.Fully to mix with Buffer solution to make sample and react, after box body 600 is carried out rocking 10 seconds, then box body 600 being left standstill 2 minutes.Then, as shown in figure 17, open valve 200, switch 201 position now on valve as shown in figure 12.Now, through the rotation of switch, the one end open of the passage in valve 200 or hole 802 to go at the bottom of sample chamber chamber 102 and export 107 places, be communicated with sample chamber, 801 holes then go to 802 original hole site places, namely communicate with the entrance of test chamber 300 or passage 205, like this, C hole 801 is communicated with test sample chamber 300.After then sample chamber 100 is communicated with by the passage 800 of valve 200 with test chamber 300, sample mixed liquor in sample chamber 100 is because of the gas pressure in chamber, force segment fluid flow flow into test chamber 300 along valve 200 and contact with the uptake zone 3011 of test strips 301, test strips is tested.
Identical in order to ensure the air pressure produced in sample cavity 100, the distance of lid 500 movement on the opening 101 of cavity 100 can be allowed identical, allow the air pressure that produces in the sample chamber in each different device identical like this, thus the volume forcing the fluid exhaust chamber in cavity external is also identical, the performance of assurance device is homogeneous and stable.Such as can arrange in lid with the number of screw thread on opening 101 and the degree of depth, at accent place 101 arranged outside raised structures 109 of cavity 100, the displacement of lid 500 at sample chamber accent 101 place can also be controlled.
When sample be solid or semisolid time, sample, after mixing with Buffer solution, has suspension and is present in mixed liquor.In order to ensure the accuracy of pattern detection, can a filtration unit 302 being set between valve 200 and test chamber 300, being used for the suspension filtered out in mixed liquor, as filter paper, filter etc.The sample such as allowing the fluid from passage 205 flow to testing element through filter pad 302 applies on position 3011.
Claims (17)
1. collect a device for fluid sample, comprising:
Sample chamber;
Control the device of fluid circulation;
The device that sample chamber circulates with control fluid is connected;
Increase atmospheric device, for increasing the air pressure in sample chamber; The device controlling fluid circulation comprises valve and holds the valve cavity of valve, and wherein the second opening of sample chamber communicates with valve cavity; Valve has the primary importance of sealing sample chamber second opening and opens the second place of sample chamber second opening in valve cavity;
When valve is positioned at primary importance time, the air pressure in sample chamber increases; When valve is positioned at the second place time, the air pressure of increase forces fluid sample to be flowed out by the second opening.
2. collect the device of fluid sample as claimed in claim 1, it is characterized in that, the air pressure of the increase in sample chamber forces fluid sample to flow out to outside sample chamber.
3. collect the device of fluid sample as claimed in claim 1, it is characterized in that, sample chamber comprises the first and second openings, and wherein the first opening is used for receiving sample; The device that second opening circulates with control fluid is connected.
4. collect the device of fluid sample as claimed in claim 3, it is characterized in that, increase the first opening that atmospheric device is used for engaging sample chamber.
5. collect the device of fluid sample as claimed in claim 1, it is characterized in that, valve exterior surface and valve cavity inner wall surface are line contact sealing.
6. collect the device of fluid sample as claimed in claim 1, the fluid sample flowed out by sample chamber second opening enters into a test chamber.
7. collect the device of fluid sample as claimed in claim 1, this device also comprises a test chamber, and whether the fluid sample that the device controlling fluid circulation controls in sample chamber enters into test chamber.
8. collect the device of fluid sample as claimed in claim 1, this device also comprises a sample storage chamber for exact p-value, and whether the fluid sample that the device controlling fluid circulation controls in sample chamber enters into storage chamber.
9. as described in claim 1 or 4, collect the device of fluid sample, it is characterized in that, increasing atmospheric device is seal element.
10. collect the device of fluid sample as claimed in claim 9, it is characterized in that, seal element and sample chamber madial wall form hermetic seal.
11. devices collecting fluid sample as claimed in claim 10, it is characterized in that, this device also comprises a lid, with sealing element on lid.
12. devices collecting fluid sample as claimed in claim 1, is characterized in that, comprise the reagent of process sample in sample chamber, wherein reagent is buffer solution.
13. devices collecting fluid sample as claimed in claim 1, it is characterized in that, sample chamber is funnel shaped structure.
14. devices collecting fluid sample as claimed in claim 1, it is characterized in that, described sample is saliva.
15. 1 kinds of boxes, comprising: a kind of device collecting fluid sample, and this device comprises: sample chamber; Control the device of fluid circulation; The device that sample chamber circulates with control fluid is connected; Increase atmospheric device, for increasing the air pressure in sample chamber; The device controlling fluid circulation comprises valve and holds the valve cavity of valve, and wherein the second opening of sample chamber communicates with valve cavity; Valve has the primary importance of sealing sample chamber second opening and opens the second place of sample chamber second opening in valve cavity; When valve is positioned at primary importance time, the air pressure in sample chamber increases; When valve is positioned at the second place time, the air pressure of increase forces fluid sample to be flowed out by the second opening, and sample collection rod.
16. boxes as claimed in claim 15, is characterized in that, described sample collection rod comprises the cotton swab for collecting sample.
17. boxes as claimed in claim 15, is characterized in that, increasing atmospheric device is seal element, and sealing element is positioned on sample collection rod.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010538076.1A CN102466581B (en) | 2010-11-09 | 2010-11-09 | A kind of device |
| CN201510511762.2A CN105115798B (en) | 2010-11-09 | 2010-11-09 | A kind of method for handling sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010538076.1A CN102466581B (en) | 2010-11-09 | 2010-11-09 | A kind of device |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510511762.2A Division CN105115798B (en) | 2010-11-09 | 2010-11-09 | A kind of method for handling sample |
Publications (2)
| Publication Number | Publication Date |
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| CN102466581A CN102466581A (en) | 2012-05-23 |
| CN102466581B true CN102466581B (en) | 2015-09-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510511762.2A Expired - Fee Related CN105115798B (en) | 2010-11-09 | 2010-11-09 | A kind of method for handling sample |
| CN201010538076.1A Expired - Fee Related CN102466581B (en) | 2010-11-09 | 2010-11-09 | A kind of device |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201510511762.2A Expired - Fee Related CN105115798B (en) | 2010-11-09 | 2010-11-09 | A kind of method for handling sample |
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| CN (2) | CN105115798B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103575575A (en) * | 2012-07-23 | 2014-02-12 | 艾博生物医药(杭州)有限公司 | Device |
| CN103776660B (en) * | 2012-10-24 | 2016-03-09 | 艾博生物医药(杭州)有限公司 | A kind of device |
| CN104634611B (en) * | 2013-11-14 | 2018-07-13 | 艾博生物医药(杭州)有限公司 | A method of the device of quantitative shunting is carried out to liquid sample and uses the device |
| US9995743B2 (en) | 2015-07-01 | 2018-06-12 | Htc Corporation | Test apparatus and pressurizing assembly thereof |
| CN112697552B (en) * | 2020-12-09 | 2023-02-17 | 青岛市妇女儿童医院 | Smear device for clinical examination |
| CN114151546B (en) * | 2021-11-19 | 2024-01-05 | 奥星制药设备（石家庄）有限公司 | Flour mill |
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| EP0508010A1 (en) * | 1991-04-10 | 1992-10-14 | La Mina Ltd. | Biological fluids testing membrane module and method of conducting same |
| CN1509409A (en) * | 2001-05-18 | 2004-06-30 | ����ʵ�鼼����˾ | In-line test device and methods of use |
| US7341669B2 (en) * | 2002-10-11 | 2008-03-11 | G6 Science Corp. | Filter methods to capture a desired amount of material from a sample suspension for monolayer deposition, analysis or other uses |
| CN201596509U (en) * | 2006-03-29 | 2010-10-06 | 因弗因斯医药瑞士股份有限公司 | Analysis device |
| CN201607358U (en) * | 2009-12-11 | 2010-10-13 | 上海快德行生物技术有限公司 | Mixing device of medical testing sample |
| CN202119661U (en) * | 2010-11-09 | 2012-01-18 | 艾博生物医药(杭州)有限公司 | Device and box for treating samples |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105115798A (en) | 2015-12-02 |
| CN105115798B (en) | 2018-02-02 |
| CN102466581A (en) | 2012-05-23 |
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