CN104226384B - Liquid-discharging means of bottle - Google Patents

Liquid-discharging means of bottle Download PDF

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Publication number
CN104226384B
CN104226384B CN201310222633.2A CN201310222633A CN104226384B CN 104226384 B CN104226384 B CN 104226384B CN 201310222633 A CN201310222633 A CN 201310222633A CN 104226384 B CN104226384 B CN 104226384B
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liquid
adsorption layer
guiding gutter
bottle
diversion column
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CN104226384A (en
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张冠斌
赵凯军
盛涛
毛吉扬
陈铖
邢婉丽
程京
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Tsinghua University
CapitalBio Corp
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Tsinghua University
CapitalBio Corp
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Abstract

The invention discloses a kind of liquid-discharging means of bottle.It comprises at least 1 device A and at least 1 device B; Each described device A is following 1) or 2) device: 1) by 1 diversion column a(2a) and at least 1 capillary (1) form; Described diversion column a is cone body, and described capillary is axially across described diversion column a and be fixed on described diversion column a, and one end of described capillary protrudes from the top of described diversion column a; 2) be 1 diversion column b(2b); Described diversion column b is the pointed cone body in top, and its side surface is provided with some grooves (9) extending longitudinally the end of to from top; The liquid-adsorption layer (4) that each described device B comprises 1 guiding gutter (3) and is layed in this guiding gutter; The bottom of each described device A is located in the described guiding gutter in 1 described device B, and contacts with the described liquid-adsorption layer in this device B or mutually close.Liquid-discharging means of bottle of the present invention have use simple, convenient, detect advantage fast.

Description

Liquid-discharging means of bottle
Technical field
The present invention relates to a kind of liquid-discharging means of bottle, be applicable to transfer and the detection of micro liquid.
Background technology
In experiment in the field such as biology, chemistry, medical science, molecular diagnosis and detection often need the liquid in EP pipe, PCR pipe or centrifuge tube take out and shift, the method of conventional transfer operation is a lot, as drawn transfer etc. by pipettor or syringe, minority also can be realized by the absorption of tunica fibrosa or test strips.Because the inwall of EP pipe, PCR pipe and centrifuge tube has suction-operated, when the liquid of the inside is less, be difficult to flow out, even if pipe is inverted, liquid in pipe also can not flow out automatically.If directly use tunica fibrosa or test paper directly to dip liquid in pipe, then need to extend in pipe, and some experimental liquids are as amplification in nucleic acid or the nonamplifie solution containing specific gene fragment, human serum solution or containing antibodies buffer in protide, its physical environment needs are closed and are not easy to directly open, and this makes the liquid directly utilizing EP pipe, PCR pipe, centrifuge tube to encapsulate receive restriction for portable detection or diagnosis.
Summary of the invention
The object of this invention is to provide a kind of liquid-discharging means of bottle, this device not only solves the problem that reaction liquid in pipe not easily draws transfer, also the liquid of transfer can be detected fast.
Liquid-discharging means of bottle provided by the present invention comprises at least 1 device A and at least 1 device B;
Each described device A is following 1) or 2) device:
1) be made up of 1 diversion column a and at least 1 capillary; Described diversion column a is cone body, and described capillary is axially across described diversion column a and be fixed on described diversion column a, and one end of described capillary protrudes from the top of described diversion column a;
2) be 1 diversion column b; Described diversion column b is the pointed cone body in top, and its side surface is provided with some grooves extending longitudinally end of to from top;
Each described device B comprises 1 guiding gutter and is layed in the liquid-adsorption layer in this guiding gutter; Described laying specifically can be dismountable, is convenient to change liquid-adsorption layer at any time;
The bottom of each described device A is located in the described guiding gutter in 1 described device B, and contacts with the described liquid-adsorption layer in this device B or mutually close; So that the liquid got off by water conservancy diversion is shifted by described liquid-adsorption layer.
When less for the liquid of water conservancy diversion and when being contained in the less reaction tube in bottom surface, described device A can be described device 1); When more for the liquid of water conservancy diversion and when being contained in the larger reaction tube in bottom surface, described device A can be described device 2); Described reaction tube can be EP pipe, PCR pipe or centrifuge tube;
Described device A is for puncturing the pipe lid of described reaction tube mouth of pipe place sealing and the liquid in reaction tube being imported the liquid-adsorption layer of described device B; Material such as the aluminium-foil paper that the pipe Gai Youyi of described reaction tube punctures is made;
Described guiding gutter is used for placing and fixing described liquid-adsorption layer; Described liquid-adsorption layer Absorbable rod, release and transfer liquid.
In aforesaid liquid guiding device, in the guiding gutter in each described device B, also can be provided with at least 1 reaction zone; Described reaction zone is the region that will react for conducting fluid;
Described liquid-adsorption layer in described reaction zone can be Test paper, and for detecting the liquid for water conservancy diversion, the described liquid-adsorption layer in other region can be blotting paper, for transfer liquid, specifically can arrange according to actual needs;
And/or, the guiding gutter bottom surface below the described liquid-adsorption layer in described reaction zone can be provided with air-dry detection thing;
And/or, the guiding gutter bottom surface below the described liquid-adsorption layer in described reaction zone is provided with several boss; Described boss also can be provided with air-dry detection thing; Described boss can make the liquid of the detection thing on it preferentially and in liquid-adsorption layer come in contact and react.
And/or, the sidewall of the guiding gutter in described reaction zone is provided with that some and described guiding gutter communicates, that opening is positioned at described liquid-adsorption layer lower surface place cavity; Air-dry detection thing also can be provided with in described cavity; Described cavity is positioned at the side of described liquid-adsorption layer, observing response result at any time of being more convenient for;
Described detection thing is the material reacted with the liquid for water conservancy diversion.
In aforesaid liquid guiding device, above the described guiding gutter in described reaction zone, be provided with the lid that can extrude described liquid-adsorption layer, it can be squeezed out from liquid-adsorption layer when the liquid that goes out of water conservancy diversion is less; Described lid can be fixed on described guiding gutter, also can be set to detachable, can be made up of hard material, also can be made up of elastomeric material.
In aforesaid liquid guiding device, the axis bearing of trend along each described diversion column a is provided with a draw-in groove, and described capillary is fixedly stuck in described draw-in groove; Each described capillary is straight tube.
In aforesaid liquid guiding device, each described guiding gutter and/or each described cavity are made up of transparent material, directly to observe the flow locations of liquid or reaction, diagnosis and detection result; Each described guiding gutter can be arranged according to actual needs, can be circle, rectangle or other shape; The shape of each described cavity also can be arranged according to actual needs, can be spheroid, cuboid or other shape;
In aforesaid liquid guiding device, each described guiding gutter bottom surface extends respectively to two orthogonal planes or extends in same plane, also can extend in other any direction in space, so that observation experiment result, also can arrange according to other actual conditions.
In aforesaid liquid guiding device, when being provided with 2 the above device A in the guiding gutter of each described device B, described liquid-adsorption layer can extend in branch-like, and different from the described liquid-adsorption layer respectively described branch in the bottom of device A described in two or more contacts or mutually close.
In aforesaid liquid guiding device, the height of each described diversion column a and each described diversion column b can be 10-50mm, and the length of each described capillary can be 10-60mm, specifically can the degree of depth residing for reaction liquid in pipe determine.
In aforesaid liquid guiding device, the external diameter of each described capillary can be 0.5mm-2.5mm, and internal diameter can be 0.45mm-2mm.
The present invention also provides a kind of liquid guide flow method, comprises the steps:
By downward for the reaction tube mouth of pipe that the sealing of liquid to be transferred is housed, puncture the reaction tube mouth of pipe of described sealing with the described device A in above-mentioned arbitrary described liquid-discharging means of bottle; Described capillary in described device A or diversion column b are stretched into the liquid place to be transferred in described reaction tube, liquid to be transferred is imported in the liquid-adsorption layer in above-mentioned arbitrary described liquid-discharging means of bottle;
The described reaction tube mouth of pipe specifically can be sealed by aluminium-foil paper.
The present invention also provides a kind of liquid detecting method, comprises the steps:
The detection liquid of liquid to be measured and the described liquid to be measured of detection is reacted according in the liquid-adsorption layer comprised in the above-mentioned arbitrary described liquid-discharging means of bottle of described liquid guide flow method importing, obtains testing result.
The present invention also provides another kind of liquid detecting method, comprises the steps:
By liquid to be measured according in the liquid-adsorption layer comprised in the above-mentioned arbitrary described liquid-discharging means of bottle of described liquid guide flow method importing, react with the detection thing be provided with in described reaction zone, obtain testing result;
When the liquid to be measured in described liquid-adsorption layer is not enough to react with the detection thing in the reaction zone in above-mentioned arbitrary described liquid-discharging means of bottle, also comprise the step of the liquid-adsorption layer of extruding containing described liquid to be measured;
Described extruding specifically realizes by pressing described lid directly to the liquid-adsorption layer direction containing described liquid to be measured.
Liquid-discharging means of bottle provided by the present invention have use simple, convenient, detect advantage fast, can be widely used in chemistry and life science portable inspectiont and in diagnosing.
Accompanying drawing explanation
Fig. 1 is the side view of embodiment 1 liquid-discharging means of bottle.
Fig. 2 is the use schematic diagram of embodiment 1 liquid-discharging means of bottle.
Fig. 3 is the side view of embodiment 2 liquid-discharging means of bottle and uses schematic diagram.
Fig. 4 is the side view of embodiment 3 liquid-discharging means of bottle and uses schematic diagram.
Fig. 5 is the side view of embodiment 4 liquid-discharging means of bottle and uses schematic diagram.
Fig. 6 be embodiment 5 liquid-discharging means of bottle side view, use the sectional view at schematic diagram and A-A line place.
Fig. 7 be embodiment 6 liquid-discharging means of bottle side view, use the sectional view at schematic diagram and B-B line place.
Fig. 8 is the side view of cavity in liquid-discharging means of bottle.
In above-mentioned Fig. 1-8,1 is capillary, and 2a is diversion column a, 2b is or diversion column b that 3 is guiding gutter, and 4 is liquid-adsorption layer, and 5 and 6 is reaction tube, and 7 is cavity, and 8 is boss, and 9 is groove, and 10 is draw-in groove, and 11 is lid; Double-head arrow straight down represents direction firmly.
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1, liquid-discharging means of bottle
As shown in Figure 1, the liquid-discharging means of bottle that the present embodiment provides comprises 1 device A and 1 device B;
Described device A is made up of 1 diversion column 2a and 1 capillary 1; Described diversion column 2a is cone body, and described capillary 1 is axially across described diversion column 2a and be fixed on described diversion column 2a, and one end of described capillary 1 protrudes from the top of described diversion column 2a;
Described device B is made up of guiding gutter 3 and the liquid-adsorption layer 4 be layed in this guiding gutter 3; Described be laid in dismountable; The bottom surface of described guiding gutter 3 extends respectively to orthogonal two planes;
1 reaction zone is provided with perpendicular in the guiding gutter 3 of horizontal plane in described device B; Described liquid-adsorption layer in described reaction zone is Test paper, so that observed result.
The bottom of described device A is located in the described guiding gutter 3 in described device B, and contacts with the described liquid-adsorption layer 4 in this device B; The degree of depth of described guiding gutter 3 is 1.5mm;
The axis bearing of trend of described diversion column 2a is provided with a draw-in groove 10, and described capillary 1 is fixedly stuck in described draw-in groove 10; The height of described diversion column 2a is 10mm;
Described capillary 1 is straight tube, its bearing of trend and described liquid-adsorption layer 4 perpendicular; The external diameter of described capillary 1 is 0.5mm, internal diameter is 0.45mm, length is 10mm; And capillary tip exceeds diversion column top 2mm;
Described capillary 1 is made up of glass; Described diversion column 2a is made of plastics; Described guiding gutter 3 is made up of transparent rigid plastics so that observing response result; Described liquid-adsorption layer 4 in described reaction zone makes by Test paper the liquid detecting water conservancy diversion.
The using method of the described liquid-discharging means of bottle that the present embodiment provides is as follows:
As shown in Figure 2, reaction tube 5 mouth of pipe aluminium-foil paper that liquid to be transferred or to be detected is housed is closed, the reaction tube 5 of sealing is inverted, aluminium-foil paper is punctured with device A, and the liquid place to be transferred or to be detected capillary 1 stretched into bottom reaction tube 5, liquid flows into liquid-adsorption layer 4 along capillary, and liquid-adsorption layer 4 adsorptive liquid also makes liquid flow to reaction zone (liquid-adsorption layer be made up of Test paper namely in reaction zone) along guiding gutter 3, observing response result.
Embodiment 2, liquid-discharging means of bottle
As shown in Figure 3, the liquid-discharging means of bottle that the present embodiment provides comprises following 2 device A and 1 device B:
Wherein, a described device A is made up of 1 diversion column 2a and 1 capillary 1, and described diversion column 2a is cone body, and described capillary 1 is axially across described diversion column 2a and be fixed on described diversion column 2a; And one end of described capillary 1 protrudes from the top of described diversion column 2a;
Device A described in another is 1 diversion column 2b; Described diversion column 2b is the pointed cone body in top, and its side surface is evenly provided with 4 grooves 9 extending longitudinally end of to from top;
The liquid-adsorption layer 4 that described device B comprises 1 guiding gutter 3 and is layed in this guiding gutter 3; Described be laid in dismountable; The place plane of described guiding gutter 3 bottom land extends respectively to two orthogonal planes; Described guiding gutter 3 comprises a sprue and 2 branches in horizontal plane;
The bottom of a described device A is located in a branch of described guiding gutter 3, and contacts with the liquid-adsorption layer 4 in sprue; The bottom of device A described in another is located in another branch of described guiding gutter 3, and mutually close with the described liquid-adsorption layer 4 in this branch; The degree of depth of described guiding gutter 3 is 1.5mm;
Axis bearing of trend along described diversion column 2a is provided with a draw-in groove 10, and described capillary 1 is fixedly stuck in described draw-in groove 10; The height of each described diversion column 2a and 2b is 50mm;
Described capillary 1 is straight tube, its bearing of trend and described liquid-adsorption layer 4 perpendicular; The external diameter of described capillary 1 is 2.5mm, internal diameter is 2.0mm, length is 60.0mm; Higher than diversion column 2a10mm;
Described capillary 1 is made up of glass; Described diversion column 2a and 2b is all made of plastics; Described guiding gutter 3 is made up of transparent rigid plastics; Described liquid-adsorption layer 4 is made up of blotting paper.
The using method of the described liquid-discharging means of bottle that the present embodiment provides is as follows:
As shown in Figure 3, the mouth of pipe of two reaction tubes 5 and 6 that liquid is housed is sealed with aluminium-foil paper respectively, one of them reaction tube is built with liquid to be detected, another is equipped with detection liquid, two reaction tubes sealed are inverted, aluminium-foil paper is punctured respectively with two device A, and capillary 1 and diversion column 2b are stretched into the liquid place bottom reaction tube 5 and 6 respectively, liquid flows into liquid-adsorption layer 4 along capillary, liquid to be detected and detection liquid flow into guiding gutter sprue along the branch of guiding gutter respectively, and there is chromogenic reaction, observing response result.
Embodiment 3, liquid-discharging means of bottle
As shown in Figure 4, the liquid-discharging means of bottle that the present embodiment provides comprises 1 device A and 1 device B;
Described device A is made up of 1 diversion column 2a and 1 capillary 1, and described diversion column 2a is cone body, and described capillary 1 is axially across described diversion column 2a and be fixed on described diversion column 2a, and one end of described capillary 1 protrudes from the top of described diversion column 2a;
The liquid-adsorption layer 4 that described device B comprises 1 guiding gutter 3 and is layed in this guiding gutter 3; Described be laid in dismountable; The bottom surface of described guiding gutter 3 extends in same plane; Described liquid-adsorption layer 4 part covers guiding gutter 3; In described guiding gutter 3, reaction zone is set, is namely provided with air-dry detection thing in the below of the liquid-adsorption layer 4 of reaction zone;
The bottom of described device A is located in the region that described guiding gutter 3 do not cover by liquid-adsorption layer 4, and contacts with the described liquid-adsorption layer 4 in this device B; The degree of depth of described guiding gutter 3 is 1.5mm;
The axis bearing of trend of described diversion column 2a is provided with a draw-in groove 10, and described capillary 1 is fixedly stuck in described draw-in groove 10; The height of described diversion column 2a is 15.5mm;
Described capillary 1 is straight tube, its bearing of trend and described liquid-adsorption layer 4 perpendicular; The external diameter of described capillary 1 is 1.0mm, internal diameter is 0.7mm, length is 20.0mm;
Described capillary 1 is made up of glass; Described diversion column 2a is made of plastics; Described guiding gutter 3 is made up of transparent rigid plastics; Described liquid-adsorption layer 4 is being made up of blotting paper.
The using method of the described liquid-discharging means of bottle that the present embodiment provides is as follows:
As shown in Figure 4, reaction tube 5 mouth of pipe aluminium-foil paper that liquid to be transferred or to be detected is housed is closed, the reaction tube 5 of sealing is inverted, aluminium-foil paper is punctured with device A, and the liquid place to be transferred or to be detected capillary 1 stretched into bottom reaction tube 5, liquid flows into liquid-adsorption layer 4 along capillary, and liquid-adsorption layer 4 adsorptive liquid also makes liquid flow to reaction zone (being provided with air-dry detection thing below the liquid-adsorption layer of reaction zone) along guiding gutter 3, observing response result.
Embodiment 4, liquid-discharging means of bottle
As shown in Figure 5, the liquid-discharging means of bottle that the present embodiment provides comprises following 2 device A and 1 device B:
Each described device A is made up of 1 diversion column 2a and 1 capillary 1, described diversion column 2a is a cone body, described capillary 1 is axially across described diversion column 2a and be fixed on described diversion column 2a, and one end of described capillary 1 protrudes from the top of described diversion column 2a;
The liquid-adsorption layer 4 that described device B comprises 1 guiding gutter 3 and is layed in this guiding gutter 3; Described be laid in dismountable; The bottom surface of described guiding gutter 3 extends in same plane; Described liquid-adsorption layer 4 part covers guiding gutter 3;
The bottom of the diversion column 2a in each described device A is located in the region that described guiding gutter 3 do not cover by liquid-adsorption layer 4, and contacts with the described liquid-adsorption layer 4 in this device B; The degree of depth of described guiding gutter 3 is 1.5mm;
The axis bearing of trend of each described diversion column 2a is equipped with a draw-in groove 10, and each described capillary 1 is fixedly stuck in each described draw-in groove 10; The height of described diversion column 2a is 24.5mm;
Each described capillary 1 is straight tube, its bearing of trend and described liquid-adsorption layer 4 perpendicular; The external diameter of each described capillary 1 is 1.2mm, internal diameter is 1.0mm, length is 25.0mm;
Described capillary 1 is made up of glass; Each described diversion column 2a is made of plastics; Described guiding gutter 3 is made up of transparent rigid plastics; Described liquid-adsorption layer 4 is made up of blotting paper.
The using method of the described liquid-discharging means of bottle that the present embodiment provides is as follows:
As shown in Figure 5, the mouth of pipe of two reaction tubes 5 that liquid is housed is sealed with aluminium-foil paper respectively, one of them reaction tube is built with liquid to be detected, two reaction tubes sealed, built with detection liquid, are inverted, are punctured aluminium-foil paper respectively with two device A by another reaction tube, and two capillaries 1 are stretched into respectively the liquid place of two reaction tubes 5, liquid to be detected and detection liquid flow in liquid-adsorption layer respectively, and chromogenic reaction occur, observing response result.
Embodiment 5, liquid-discharging means of bottle
As shown in Figure 6, the liquid-discharging means of bottle that the present embodiment provides is close with embodiment 3, and difference is: guiding gutter 3 bottom surface below the described liquid-adsorption layer 4 in described reaction zone is provided with several boss 8; Described boss 8 is provided with detection thing; Described detection thing is the material reacted with the liquid for water conservancy diversion.
The using method of the described liquid-discharging means of bottle that the present embodiment provides is as follows:
Reaction tube 5 mouth of pipe aluminium-foil paper that liquid to be transferred or to be detected is housed is closed, the reaction tube of sealing is inverted, aluminium-foil paper is punctured with device A, and the liquid place to be transferred or to be detected capillary 1 stretched into bottom reaction tube 5, liquid flows into liquid-adsorption layer 4 along capillary, liquid-adsorption layer 4 adsorptive liquid also makes liquid flow to reaction zone along guiding gutter 3, reacts with the detection thing on reaction zone inner convex platform 8, observing response result.
Embodiment 6, liquid-discharging means of bottle
As shown in Figure 7, the liquid-discharging means of bottle that the present embodiment provides is close with embodiment 3, and difference is: the sidewall both sides of the guiding gutter 3 in described reaction zone are respectively equipped with the cavity 7(that 2 openings communicated with described guiding gutter 3 are arranged in described liquid-adsorption layer 4 lower surface place and also can be provided with several cavitys 7 in the sidewall side of the guiding gutter 3 of described reaction zone as shown in Figure 8); And be provided with dismountable lid 11 extruding described liquid-adsorption layer above described guiding gutter 3 in described reaction zone; Detection thing is provided with in described cavity 7; Described detection thing is the material reacted with the liquid for water conservancy diversion.Cavity 7 and lid 11 are made up of transparent rigid plastics.
The using method of the described liquid-discharging means of bottle that the present embodiment provides is as follows:
Reaction tube 5 mouth of pipe aluminium-foil paper that liquid to be transferred or to be detected is housed is closed, the reaction tube of sealing is inverted, aluminium-foil paper is punctured with device A, and the liquid place to be transferred or to be detected capillary 1 stretched into bottom reaction tube 5, liquid flows into liquid-adsorption layer 4 along capillary, liquid-adsorption layer 4 adsorptive liquid also makes liquid flow to reaction zone along guiding gutter 3, described lid 11 is used to extrude liquid-adsorption layer 4, the liquid in it is made to flow into described cavity 7, and react with the detection thing in described cavity 7, observing response result.

Claims (9)

1. liquid-discharging means of bottle, is characterized in that: it comprises at least 1 device A and at least 1 device B;
Each described device A is following 1) or 2) device:
1) be made up of 1 diversion column a (2a) and at least 1 capillary (1); Described diversion column a is cone body, and described capillary is axially across described diversion column a and be fixed on described diversion column a, and one end of described capillary protrudes from the top of described diversion column a;
2) be 1 diversion column b (2b); Described diversion column b is the pointed cone body in top, and its side surface is provided with some grooves (9) extending longitudinally the end of to from top;
The liquid-adsorption layer (4) that each described device B comprises 1 guiding gutter (3) and is layed in this guiding gutter;
The bottom of each described device A is located in the described guiding gutter in 1 described device B, and contacts with the described liquid-adsorption layer in this device B or mutually close;
At least 1 reaction zone is provided with in guiding gutter in each described device B;
Described liquid-adsorption layer in described reaction zone is Test paper;
And/or, the guiding gutter bottom surface below the described liquid-adsorption layer in described reaction zone is provided with air-dry detection thing;
And/or, the guiding gutter bottom surface below the described liquid-adsorption layer in described reaction zone is provided with several boss (8);
And/or, the sidewall of the guiding gutter in described reaction zone is provided with that some and described guiding gutter communicates, that opening is positioned at described liquid-adsorption layer lower surface place cavity (7).
2. liquid-discharging means of bottle according to claim 1, is characterized in that: be provided with the lid (11) that can extrude described liquid-adsorption layer above the described guiding gutter in described reaction zone.
3. liquid-discharging means of bottle according to claim 1 and 2, is characterized in that: described boss is provided with detection thing; Detection thing is provided with in described cavity; Described detection thing is the material reacted with the liquid for water conservancy diversion.
4., according to described liquid-discharging means of bottle arbitrary in claim 1 or 2, it is characterized in that: the axis bearing of trend along each described diversion column a is provided with a draw-in groove (10), and described capillary is fixedly stuck in described draw-in groove; Each described capillary is straight tube;
And/or each described guiding gutter and/or each described cavity are made up of transparent material;
And/or each described guiding gutter bottom surface extends respectively to two orthogonal planes or extends in same plane.
5. liquid-discharging means of bottle according to claim 1 and 2, it is characterized in that: when being provided with 2 the above device A in the guiding gutter of each described device B, described liquid-adsorption layer is that branch-like extends, and different from the described liquid-adsorption layer respectively described branch in the bottom of device A described in two or more contacts or mutually close.
6. liquid-discharging means of bottle according to claim 1 and 2, is characterized in that: the external diameter of each described capillary is 0.5mm-2.5mm, and internal diameter is 0.45mm-2mm;
And/or the height of each described diversion column a and each described diversion column b is 10-50mm, the length of each described capillary is 10-60mm.
7. a liquid guide flow method, comprises the steps:
By downward for the reaction tube mouth of pipe that the sealing of liquid to be transferred is housed, puncture the reaction tube mouth of pipe of described sealing with the described device A in described liquid-discharging means of bottle arbitrary in claim 1-6; Described capillary in described device A or diversion column b are stretched into the liquid place to be transferred in described reaction tube, liquid to be transferred is imported in the liquid-adsorption layer in claim 1-6 in arbitrary described liquid-discharging means of bottle;
The described reaction tube mouth of pipe can be sealed by aluminium-foil paper.
8. a liquid detecting method, comprises the steps:
The detection liquid of liquid to be measured and the described liquid to be measured of detection is reacted according in the liquid-adsorption layer comprised in method importing claim 1-6 described in claim 7 in arbitrary described liquid-discharging means of bottle, obtains testing result.
9. a liquid detecting method, comprises the steps:
By liquid to be measured according in the liquid-adsorption layer comprised in method importing claim 1-6 described in claim 7 or 8 in arbitrary described liquid-discharging means of bottle, react with the detection thing be provided with in described reaction zone, obtain testing result;
When the liquid to be measured in described liquid-adsorption layer is not enough to react with the detection thing in the reaction zone in arbitrary described liquid-discharging means of bottle in claim 1-6, also comprise the step of the liquid-adsorption layer of extruding containing described liquid to be measured;
Described being pressed through realizes directly to the lid in liquid-discharging means of bottle described in the liquid-adsorption layer direction pressing claim 2 containing described liquid to be measured.
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