CN115462389B - Soybean seed dressing agent and preparation method thereof - Google Patents

Soybean seed dressing agent and preparation method thereof Download PDF

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CN115462389B
CN115462389B CN202211322270.5A CN202211322270A CN115462389B CN 115462389 B CN115462389 B CN 115462389B CN 202211322270 A CN202211322270 A CN 202211322270A CN 115462389 B CN115462389 B CN 115462389B
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sodium alginate
seed dressing
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rhizobia
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CN115462389A (en
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张晓艳
董博
陈光荣
张芮
王立明
陈晓莉
杨如萍
张恺东
孙鹏洲
马文军
王晓菲
田建霞
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Gansu Modern Agricultural Development Co ltd
DRYLAND AGRICULTURE INSTITUTE GANSU ACADEMY OF AGRICULTURAL SCIENCES
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Gansu Modern Agricultural Development Co ltd
DRYLAND AGRICULTURE INSTITUTE GANSU ACADEMY OF AGRICULTURAL SCIENCES
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N51/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00Disinfectants; Antimicrobial compounds or mixtures thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00Arthropodicides
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Insects & Arthropods (AREA)
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Abstract

The application relates to a soybean seed dressing agent and a preparation method thereof, and the soybean seed dressing agent is characterized by comprising an insecticide, a bactericide and coated microspheres; the coated microsphere contains rhizobium, and the rhizobium is slow-growing rhizobium. The application can effectively protect soybean seeds and improve the emergence rate, yield and soybean quality.

Description

Soybean seed dressing agent and preparation method thereof
Technical Field
The application relates to a soybean seed dressing agent and a preparation method thereof.
Background
At present, a bactericide and an insecticide are often added into the soybean seed dressing agent, so that damage of fungus and pests to seeds is prevented, soybean seeds are protected, and the seeds grow smoothly. Rhizobia can symbiotic with soybean root system, and can convert nitrogen in air into nitrogen which can be absorbed by soybean, and can provide nutrients for soybean, and because some soil contains no rhizobia symbiotic with leguminous plants, and at the same time, different leguminous plants need to symbiotic with different types of rhizobia, when leguminous plants are sown in agriculture, the rhizobia can be stirred with rhizobia preparation so as to create conditions for forming rhizobia for leguminous crops.
However, since the bactericide has no effect on rhizobia, the bactericide and the rhizobia can not coexist, and thus the soybean seed dressing agent can only be selected from the bactericide and the rhizobia, and even the effect of the bactericide and the rhizobia is not satisfactory, the current market lacks products capable of simultaneously adding the bactericide and the rhizobia, and the bactericide and the rhizobia can respectively exert effects, are not influenced by each other, and further the yield of soybeans is improved.
Disclosure of Invention
The application provides a soybean seed dressing agent and a preparation method thereof, which solve the technical problems that 1) bactericide and rhizobia are added simultaneously, and the effect cannot be satisfied; 2) Further improving the yield of soybean.
In order to solve the technical problems, the application adopts the following technical scheme:
a soybean seed dressing agent comprises pesticide, bactericide and coated microsphere; the coated microsphere at least comprises sodium alginate, calcium chloride, gelatin and rhizobium, wherein the rhizobium is of the genus Rhizobium.
The pesticide is one or more of carbofuran, thiamethoxam and imidacloprid; the bactericide is one or more of metalaxyl-M, fludioxonil, chlorothalonil, kasugamycin, zhongshengmycin and carbendazim.
The coated microsphere is prepared according to the following steps:
dissolving sodium alginate in a medium 1 to prepare sodium alginate solution;
grinding gelatin to 120-200 meshes to obtain gelatin particles;
dissolving calcium chloride in the medium 2 to obtain a calcium chloride solution;
adding gelatin particles into a medium 3, dispersing for 2-5 min at the temperature of 3-5 ℃ and the rotating speed of 1000-5000 rpm, and fishing out the gelatin particles to obtain dispersed gelatin particles;
mixing the dispersed gelatin particles, rhizobia and sodium alginate solution to obtain a mixed solution;
adding the calcium chloride solution into the mixed solution, stirring, and drying to obtain solidified calcium alginate;
grinding the solidified calcium alginate to obtain the coated microsphere.
The medium 1 is one or more of water, an aqueous salt solution and an aqueous alkali solution; the medium 2 is one or more of water, an aqueous salt solution and an aqueous alkali solution; the medium 3 is one or more of water, an aqueous salt solution and an aqueous alkali solution;
the mass ratio of the sodium alginate to the medium 1 is 1-2:98-99;
the mass ratio of the calcium chloride to the medium 2 is 1-2:98-99;
the mass ratio of the gelatin to the medium 3 is 1-2:98-99;
the mass ratio of the dispersed gelatin particles, rhizobia, sodium alginate solution and calcium chloride solution is 9-15:0.1-2:30-45:30-45.
The dispersed gelatin particles, rhizobia and sodium alginate solution are mixed by firstly mixing rhizobia with the dispersed gelatin particles uniformly and then mixing with the sodium alginate solution uniformly.
The grinding is to grind to 120-200 meshes.
The preparation method of the soybean seed dressing agent is characterized in that the soybean seed dressing agent is obtained by uniformly mixing insecticide, bactericide and coated microspheres;
the mass ratio of the insecticide to the bactericide to the coated microsphere is 2-98:1-97:1-97.
The application has the following beneficial technical effects:
1. the application can better play the role of rhizobia on the premise of protecting soybean seeds from being infected by bacteria.
2. The application can obviously improve the yield of soybean when being used for soybean seed dressing.
3. The application selects gelatin as pore-forming agent, provides wide living space for rhizobia, when the temperature of gelatin is 3-5 ℃,
the medium is a swelled microsphere, has larger volume, can be dissolved along with the temperature rise after being added into sodium alginate solution, is changed from solid into liquid, and becomes smaller in volume, and sodium alginate reacts with calcium chloride to generate calcium alginate gel at the moment, so that a small space is formed in the gel for rhizobia to grow due to the change of the volume difference of gelatin, and meanwhile, the dissolved gelatin is contacted with the rhizobia, so that nutrient substances are provided for the rhizobia, and the rhizobia forms population effect in the small space.
4. According to the application, sodium alginate and calcium chloride are selected to prepare calcium alginate gel, and slow rhizobium is further selected, because the slow rhizobium decomposes carbohydrates to generate alkali, and the rhizobium decomposes carbohydrates to generate acid, calcium alginate is dissolved in alkali and is insoluble in acid, if sodium alginate is only used as an envelope, the sodium alginate is dissolved in water, after soybean sowing, the rhizobium is quickly dissolved out due to the dissolution of the sodium alginate, the envelope does not have a good wrapping effect, the rhizobium is quickly contacted with a bactericide, so that a large amount of rhizobium dies, the rhizobium cannot be protected, and the calcium alginate generated by the method is insoluble in water, so that the rhizobium can be better protected, for example, the rhizobium is used for decomposing the acid generated by the carbohydrates, the calcium alginate is insoluble in the acid, the rhizobium cannot break the gel of the calcium alginate, and the rhizobium is difficult to be utilized by the rhizobium, and the calcium alginate can not be changed to the sodium alginate.
Detailed Description
The application will be further illustrated with reference to specific examples.
Example 1
A soybean seed dressing agent comprises pesticide, bactericide and coated microsphere; the coated microsphere consists of sodium alginate, calcium chloride, gelatin and rhizobium, wherein the rhizobium is soybean slow rooting rhizobium, and is purchased from a Biobw strain library and is numbered bio-16555.
The pesticide is thiamethoxam; the bactericide is kasugamycin.
The coated microsphere is prepared according to the following steps:
dissolving sodium alginate in water to obtain sodium alginate solution;
grinding gelatin to 140-160 meshes to obtain gelatin particles;
dissolving calcium chloride in water to obtain a calcium chloride solution;
adding gelatin particles into water, dispersing for 3min at the temperature of 3-5 ℃ and the rotating speed of 1200rpm, and fishing out the gelatin particles to obtain dispersed gelatin particles;
mixing the dispersed gelatin particles, rhizobia and sodium alginate solution to obtain a mixed solution;
adding the calcium chloride solution into the mixed solution, stirring, and drying to obtain solidified calcium alginate;
grinding the solidified calcium alginate to 120 meshes to obtain the coated microspheres.
The mass ratio of the sodium alginate to the water is 1.2:98.8;
the mass ratio of the calcium chloride to the water is 1.5:98.5;
the mass ratio of the gelatin to the water is 1.2:98.8;
the mass ratio of the dispersed gelatin particles, rhizobia, sodium alginate solution and calcium chloride solution is 14:0.3:45:40.7.
The preparation method of the soybean seed dressing agent comprises the steps of uniformly mixing the insecticide, the bactericide and the coated microspheres according to the mass ratio of 60:15:25, and obtaining the soybean seed dressing agent.
The thiamethoxam is 25% thiamethoxam water dispersible granule.
Example 2
A soybean seed dressing agent comprises pesticide, bactericide and coated microsphere; the coated microsphere consists of sodium alginate, calcium chloride, gelatin and rhizobium, wherein the rhizobium is soybean slow rooting rhizobium, and is purchased from a Biobw strain library and is numbered bio-16555.
The pesticide is thiamethoxam; the bactericide is kasugamycin.
The coated microsphere is prepared according to the following steps:
dissolving sodium alginate in water to obtain sodium alginate solution;
grinding gelatin to 140-160 meshes to obtain gelatin particles;
dissolving calcium chloride in water to obtain a calcium chloride solution;
adding gelatin particles into water, dispersing for 3min at the temperature of 3-5 ℃ and the rotating speed of 1200rpm, and fishing out the gelatin particles to obtain dispersed gelatin particles;
uniformly mixing rhizobia with dispersed gelatin particles, and uniformly mixing with sodium alginate solution to obtain a mixed solution;
adding the calcium chloride solution into the mixed solution, stirring, and drying to obtain solidified calcium alginate;
grinding the solidified calcium alginate to 120 meshes to obtain the coated microspheres.
The mass ratio of the sodium alginate to the water is 1.2:98.8;
the mass ratio of the calcium chloride to the water is 1.5:98.5;
the mass ratio of the gelatin to the water is 1.2:98.8;
the mass ratio of the dispersed gelatin particles, rhizobia, sodium alginate solution and calcium chloride solution is 14:0.3:45:40.7.
The preparation method of the soybean seed dressing agent comprises the steps of uniformly mixing the insecticide, the bactericide and the coated microspheres according to the mass ratio of 60:15:25, and obtaining the soybean seed dressing agent.
The thiamethoxam is 25% thiamethoxam water dispersible granule.
Example 3
A soybean seed dressing agent comprises pesticide, bactericide, diethyl aminoethyl hexanoate and coated microsphere; the coated microsphere consists of sodium alginate, calcium chloride, gelatin, glucose and rhizobium, wherein the rhizobium is soybean slow rhizobium, and is purchased from a Biobw strain library and is numbered bio-52823.
The pesticide is a composition of thiamethoxam and imidacloprid according to a mass ratio of 1:1; the bactericide is a composition of kasugamycin and fludioxonil according to a mass ratio of 1:2.
The coated microsphere is prepared according to the following steps:
dissolving sodium alginate and glucose in water to obtain sodium alginate solution;
grinding gelatin to 140-160 meshes to obtain gelatin particles;
dissolving calcium chloride in water to obtain a calcium chloride solution;
adding gelatin particles into water, dispersing for 4min at the temperature of 3-5 ℃ and the rotating speed of 1500rpm, and fishing out the gelatin particles to obtain dispersed gelatin particles;
uniformly mixing rhizobia with dispersed gelatin particles, and uniformly mixing with sodium alginate solution to obtain a mixed solution;
adding the calcium chloride solution into the mixed solution, stirring, and drying to obtain solidified calcium alginate;
grinding the solidified calcium alginate to 140 meshes to obtain the coated microspheres.
The mass ratio of the sodium alginate to the glucose to the water is 1.4:6:92.6;
the mass ratio of the calcium chloride to the water is 1.6:98.4;
the mass ratio of the gelatin to the water is 1.2:98.8;
the mass ratio of the dispersed gelatin particles, rhizobia, sodium alginate solution and calcium chloride solution is 12:0.4:46:41.6.
The preparation method of the soybean seed dressing agent comprises the step of uniformly mixing the insecticide, the bactericide, the diethyl aminoethyl hexanoate and the coated microspheres according to the mass ratio of 5:3:1:91, thus obtaining the soybean seed dressing agent.
The thiamethoxam is 25% thiamethoxam water dispersible granule.
Example 4
A soybean seed dressing agent comprises pesticide, bactericide, potassium fulvate and coated microsphere; the coated microsphere consists of sodium alginate, calcium chloride, gelatin and rhizobium, wherein the rhizobium is soybean slow rooting rhizobium, and is purchased from a Biobw strain library and is numbered bio-52823.
The pesticide is a composition of thiamethoxam and imidacloprid according to a mass ratio of 3:1; the bactericide is a composition of kasugamycin and fludioxonil according to a mass ratio of 3:2.
The coated microsphere is prepared according to the following steps:
dissolving sodium alginate in 0.9% saline water to obtain sodium alginate solution;
grinding gelatin to 140-160 meshes to obtain gelatin particles;
dissolving calcium chloride in water to obtain a calcium chloride solution;
adding gelatin particles into water, dispersing for 4min at the temperature of 3-5 ℃ and the rotating speed of 1500rpm, and fishing out the gelatin particles to obtain dispersed gelatin particles;
uniformly mixing rhizobia with dispersed gelatin particles, and uniformly mixing with sodium alginate solution to obtain a mixed solution;
adding the calcium chloride solution into the mixed solution, stirring, and drying to obtain solidified calcium alginate;
grinding the solidified calcium alginate to 160 meshes to obtain the coated microspheres.
The mass ratio of the sodium alginate to the water is 1.6:98.4;
the mass ratio of the calcium chloride to the water is 1.6:98.4;
the mass ratio of the gelatin to the water is 1.3:98.7;
the mass ratio of the dispersed gelatin particles, rhizobia, sodium alginate solution and calcium chloride solution is 15:2:44:39.
The preparation method of the soybean seed dressing agent comprises the steps of uniformly mixing an insecticide, a bactericide, potassium fulvate and coated microspheres according to a mass ratio of 60:20:18:2, and obtaining the soybean seed dressing agent.
The thiamethoxam is 25% thiamethoxam water dispersible granule.
The beneficial effects of the application are further illustrated below in conjunction with experimental data:
1.1 test sites: gao county, lanzhou, dan Dong, zhenxing village.
1.2 experimental detection: root nodule number (number), root nodule dry weight (g/10 strain), chlorophyll (SPAD), underground part dry weight (g/strain), overground part dry weight (g/strain), hundred grain weight (g) and yield (kg/mu), wherein 10 strains are counted for each cell, 30 strains are counted for each treatment, and an average value is obtained; hundred weight and yield were averaged.
1.3 test materials: comparative example 1 (except that rhizobium was rhizobium sojae, purchased from a Biobw strain pool, no. bio-53503, and No. bio-53503 was rhizobium with a good effect, which is known to be applied to fertilizers), comparative example 2 (except that the dispersed gelatin microparticles were not prepared, but gelatin was dissolved in sodium alginate solution, and other preparation methods were consistent with example 1), the seed dressing prepared in example 1, and the seed dressing prepared in example 2; soybean species in long zhong.
1.4 experimental implementation: seed dressing is carried out on soybean seeds by each test treatment (seed dressing agent prepared in comparative examples 1, 2, 1 and 2), seed dressing is carried out on soybean seeds according to the mass ratio of the seed dressing agent to the soybean seeds of 1:100, a cell comparison method is adopted for carrying out experiments, each treatment is divided into 3 cells which are parallel, the area of each cell is 33 square meters, the line length is 10 meters, 5 lines and the line spacing is 65cm, total 12 cells are arranged, compound fertilizers of 12-17-14 are applied to the cell base, the fertilizing amount of each cell is 2kg, the sowing time is 2022 years and 5 months and 10 days, the harvesting time is 9 months and 26 days, wherein the root nodule number (number), the root nodule dry weight (g/10 plant), the dry weight (g/plant) of the underground part and the dry weight (g/plant) of the overground part are 2022 years and 7 months and 15 days; the statistical time of hundred grain weight (g) and yield (kg/mu) was 2022, 9 months and 27 days.
The application is consistent with other implementations except for different treatments.
2 results and analysis
Root nodule count (number), root nodule dry weight (g/10 strain), chlorophyll (SPAD), underground part dry weight (g/strain), above ground part dry weight (g/strain), hundred grain weight (g) and yield (kg/mu), see table 1
As can be seen from Table 1, comparison 1 (except that rhizobia is soybean rhizobia, purchased from a Biobw strain library, and numbered bio-53503), which is the same as that of example 1, is that the number bio-53503 is the rhizobia with better effect which is known to be applied to fertilizer, and comparison of the data of example 1 (the soybean slow-growing rhizobia is selected), the application can obviously play the role of the rhizobia by selecting the soybean slow-growing rhizobia, increase the number of the rhizobia in the soybean root system, increase the chlorophyll content (the more chlorophyll content is, the more nitrogen is converted and absorbed by the soybean, namely the effect of the rhizobia is better), increase the conversion rate of nitrogen, and finally achieve the purpose of increasing the yield.
As can be seen from comparison of comparative example 2 (except that the dispersed gelatin particles are not prepared, but gelatin is dissolved in sodium alginate solution, and other preparation methods are the same as those of example 1), the gelatin is adopted as a pore-forming agent, so that rhizobia can form population advantages, the effect of rhizobia can be better exerted, the number of rhizobia in soybean root systems is increased, chlorophyll content is increased, nitrogen conversion rate is increased, and finally yield is increased.
As can be seen from the comparison of the data of examples 1 and 2, in example 2, in which rhizobium is uniformly mixed with dispersed gelatin particles and then uniformly mixed with sodium alginate solution, the number of rhizobium, chlorophyll content, weight of straw, hundred weight and yield are all higher than those of example 1 in which dispersed gelatin particles, rhizobium and sodium alginate solution are directly mixed, it is seen that rhizobium and dispersed gelatin particles are uniformly mixed first, and after gelatin particles are dissolved, enough space is provided for rhizobium to grow to form population effect, and meanwhile, dissolved gelatin can provide nutrients for rhizobium, so that the use has better effect.

Claims (5)

1. The soybean seed dressing agent is characterized by comprising an insecticide, a bactericide and coated microspheres; the coated microsphere at least comprises sodium alginate, calcium chloride, gelatin and rhizobium, wherein the rhizobium is of the genus rhizobium;
the coated microsphere is prepared according to the following steps:
dissolving sodium alginate in a medium 1 to prepare sodium alginate solution;
grinding gelatin to 120-200 meshes to obtain gelatin particles;
dissolving calcium chloride in the medium 2 to obtain a calcium chloride solution;
adding gelatin particles into a medium 3, dispersing for 2-5 min at the temperature of 3-5 ℃ and the rotating speed of 1000-5000 rpm, and fishing out the gelatin particles to obtain dispersed gelatin particles;
mixing the dispersed gelatin particles, rhizobia and sodium alginate solution to obtain a mixed solution;
adding the calcium chloride solution into the mixed solution, stirring, and drying to obtain solidified calcium alginate;
grinding the solidified calcium alginate to obtain coated microspheres;
the medium 1 is one or more of water, an aqueous salt solution and an aqueous alkali solution; the medium 2 is one or more of water, an aqueous salt solution and an aqueous alkali solution; the medium 3 is one or more of water, an aqueous salt solution and an aqueous alkali solution;
the mass ratio of the sodium alginate to the medium 1 is 1-2:98-99;
the mass ratio of the calcium chloride to the medium 2 is 1-2:98-99;
the mass ratio of the gelatin to the medium 3 is 1-2:98-99;
the mass ratio of the dispersed gelatin particles, rhizobia, sodium alginate solution and calcium chloride solution is 9-15:0.1-2:30-45:30-45.
2. The soybean seed dressing agent according to claim 1, wherein the insecticide is one or more of carbofuran, thiamethoxam and imidacloprid; the bactericide is one or more of metalaxyl-M, fludioxonil, chlorothalonil, kasugamycin, zhongshengmycin and carbendazim.
3. Soybean seed dressing according to claim 1 or 2, characterized in that the dispersed gelatin particles, rhizobia and sodium alginate solution are mixed by first mixing rhizobia with the dispersed gelatin particles and then with the sodium alginate solution.
4. The soybean seed dressing agent according to claim 1 or 2, wherein the grinding is to a mesh number of 120 to 200.
5. The method for preparing soybean seed dressing agent according to any one of claims 1 to 4, wherein the soybean seed dressing agent is obtained by uniformly mixing insecticide, bactericide and coated microspheres;
the mass ratio of the insecticide to the bactericide to the coated microsphere is 2-98:1-97:1-97.
CN202211322270.5A 2022-10-27 2022-10-27 Soybean seed dressing agent and preparation method thereof Active CN115462389B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101239846A (en) * 2008-03-07 2008-08-13 山东省农业科学院土壤肥料研究所 Embedded immobilization microbial fertilizer and its preparing method
CN105565979A (en) * 2015-12-22 2016-05-11 水利部牧区水利科学研究所 Leguminous seed pelleting coating

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101239846A (en) * 2008-03-07 2008-08-13 山东省农业科学院土壤肥料研究所 Embedded immobilization microbial fertilizer and its preparing method
CN105565979A (en) * 2015-12-22 2016-05-11 水利部牧区水利科学研究所 Leguminous seed pelleting coating

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