CN110746980A - Mycorrhizal fungi microcapsule seed coating agent for improving saline-alkali soil microbial community structure and preparation method thereof - Google Patents
Mycorrhizal fungi microcapsule seed coating agent for improving saline-alkali soil microbial community structure and preparation method thereof Download PDFInfo
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- CN110746980A CN110746980A CN201911191727.1A CN201911191727A CN110746980A CN 110746980 A CN110746980 A CN 110746980A CN 201911191727 A CN201911191727 A CN 201911191727A CN 110746980 A CN110746980 A CN 110746980A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
Abstract
The invention belongs to the technical field of bacterial manure, and particularly relates to a mycorrhizal fungi microcapsule seed coating agent for improving a soil microbial community structure of saline-alkali soil and a preparation method thereof. The seed coating agent comprises the following specific components: 1-1.5 parts of arbuscular mycorrhizal fungi preparation, 0.1-0.2 part of trichoderma harzianum preparation, 3-5g of xanthan gum, 1-1.2g of calcium carbonate, 0.1-0.2g of citric acid aqueous solution, 0.2-0.3g of ethyl lactate and 80-100mg of polylactic acid. According to the invention, polylactic acid, xanthan gum and the like are adopted to form double emulsion to prepare the microcapsule, the prepared seed coating agent has good film forming property and high embedding rate, the stability of the formed capsule wall is good, the activity of the microbial inoculum can be effectively protected, the seed can be directly coated, and the seed coating agent has good slow release effect, the inoculation success rate is high through the mutual synergistic effect of the arbuscular mycorrhizal fungi and the trichoderma harzianum, the crop yield is obviously improved after inoculation, and the crop morbidity is reduced.
Description
Technical Field
The invention belongs to the technical field of bacterial manure, and particularly relates to a mycorrhizal fungi microcapsule seed coating agent for improving a soil microbial community structure of saline-alkali soil and a preparation method thereof.
Background
Mycorrhiza is a common plant symbiosis in nature, and particularly a symbiont formed by mycorrhizal fungi in soil and a root system of a higher plant. The Arbuscular Mycorrhizal Fungi (AMF) are heterotrophic microorganisms widely distributed in the ecosystem of farmlands, and account for 5-50% of the biomass of the microbial system in the soil. The arbuscular mycorrhizal fungi can form a symbiont with eighty percent of plants on land, are very important functional microorganisms in soil, and have important influence on the growth and development of the plants and the structural function of plant communities. And the formation of plant mycorrhiza can obviously improve the nutrient absorption and the drought and high salt stress resistance of the host plant under the adverse conditions such as low phosphorus, drought, high salt and the like. Researches show that the AMF inoculated under certain conditions can promote the absorption and utilization of plants on phosphorus, zinc, copper and the like in soil, and has certain effect on the absorption of nitrogen, potassium, magnesium, sulfur and the like.
At present, the effective utilization rate of fertilizers such as nitrogen, phosphorus and potassium in China is low, so that not only is the resource waste caused, but also the soil quality is poor and the fertility is reduced due to the long-term application of a large amount of fertilizers, so that the quality of agricultural products is reduced and the environmental pollution is serious. The salinization of soil becomes a worldwide problem, the area of saline-alkali soil in China is large, the distribution is wide, and the salinization problem of cultivated land is increasingly serious. How to improve the utilization rate of the fertilizer, improve the soil fertility and improve the microbial community of the saline-alkali soil constitutes a problem to be solved urgently. How to reasonably improve and utilize the saline-alkali soil also has important significance on the quality and the yield of agricultural products in China.
Disclosure of Invention
Aiming at the problems of difficult improvement, unbalanced microbial flora, poor fertilizer utilization rate and the like of the prior saline-alkali soil, the invention provides a dry powder seed coating agent of mycorrhizal fungi for improving the microbial community structure of the saline-alkali soil.
The invention also provides a preparation method of the mycorrhizal fungi microcapsule seed coating agent for improving the microbial community structure of the saline-alkali soil.
The technical scheme adopted by the invention for realizing the purpose is as follows:
the invention provides a mycorrhizal fungi microcapsule seed coating agent for improving the microbial community structure of saline-alkali soil, which consists of the following raw materials in parts by weight: 1-1.5 parts of arbuscular mycorrhizal fungi preparation, 0.1-0.2 part of trichoderma harzianum preparation, 3-5g of xanthan gum, 1-1.2g of calcium carbonate, 0.1-0.2g of citric acid aqueous solution, 0.2-0.3g of ethyl lactate and 80-100mg of polylactic acid.
The molecular weight of the polylactic acid used in the invention is 2000-3000.
The invention also provides a preparation method of the mycorrhizal fungi microcapsule seed coating agent, which comprises the following steps:
(1) firstly, dissolving polylactic acid in ethyl acetate to obtain a polylactic acid-ethyl acetate mixed solution, uniformly stirring, adding a arbuscular mycorrhizal fungi preparation and a trichoderma harzianum preparation into the mixed solution, and uniformly stirring to form a primary emulsion;
(2) adding xanthan gum, calcium carbonate, citric acid water solution and ethyl lactate into the primary emulsion, stirring at 35-40 deg.C for 24 hr to obtain composite emulsion, adding sodium chloride water solution, and stirring at room temperature for 2 min;
(3) and drying the stirred product in vacuum to obtain the arbuscular mycorrhiza microcapsule seed coating agent.
Further, the feed-liquid ratio of the polylactic acid to the ethyl acetate is 1 mg: 1 mL.
Further, the volume ratio of the composite emulsion to the sodium chloride aqueous solution is 1: 5.
further, the concentration of the sodium chloride aqueous solution is 4.5 g/L.
Further, in the step (1), the stirring rate is 7000-7500 r/min.
Further, in the step (2), the stirring speed is 5000-
The cluster mycorrhizal fungi used in the invention areGlomusmosseae，Is commercially available; the Trichoderma harzianum strain used in the invention is purchased from China general microbiological culture collection center.
The invention has the beneficial effects that:
(1) according to the invention, polylactic acid, xanthan gum and the like are adopted to form a double emulsion to prepare the microcapsule, the prepared seed coating agent has good film forming property and high embedding rate, and the formed capsule wall has good stability and can effectively protect the activity of the microbial inoculum;
(2) the coating agent prepared by the invention can directly coat seeds, has a good slow-release effect, has high inoculation success rate through the mutual synergistic effect of the arbuscular mycorrhizal fungi and the trichoderma harzianum, obviously improves the crop yield after inoculation, and reduces the crop morbidity.
Detailed Description
The technical solution of the present invention is further explained and illustrated by the following specific examples.
Example 1
A mycorrhizal fungi microcapsule seed coating agent for improving the soil microbial community structure of saline-alkali soil is prepared by the following formula: 1 part of arbuscular mycorrhizal fungi preparation, 0.1 part of trichoderma harzianum preparation, 3g of xanthan gum, 1.2g of calcium carbonate, 0.1g of citric acid aqueous solution, 0.2g of ethyl lactate and 80mg of polylactic acid (the weight is 2000).
The preparation method comprises the following steps:
(1) firstly, dissolving polylactic acid in 80mL of ethyl acetate to obtain a polylactic acid-ethyl acetate mixed solution, stirring at 7000r/min for 2min, adding the arbuscular mycorrhizal fungi preparation and the Trichoderma harzianum preparation into the mixed solution, and stirring at 7000r/min for 2min to form a primary emulsion;
(2) adding xanthan gum, calcium carbonate, citric acid aqueous solution and ethyl lactate into the primary emulsion, stirring for 24h at 35-40 ℃ to obtain composite emulsion, adding 4.5g/L sodium chloride aqueous solution, and stirring for 2min at room temperature at 5000 r/min;
(3) and drying the stirred product in vacuum to obtain the arbuscular mycorrhiza microcapsule seed coating agent.
Example 2
A mycorrhizal fungi microcapsule seed coating agent for improving the soil microbial community structure of saline-alkali soil is prepared by the following formula: 1.2 parts of arbuscular mycorrhizal fungi preparation, 0.2 parts of trichoderma harzianum preparation, 4g of xanthan gum, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate and 100mg of polylactic acid (the weight is 3000).
The preparation method is the same as example 1.
Example 3
A mycorrhizal fungi microcapsule seed coating agent for improving the soil microbial community structure of saline-alkali soil is prepared by the following formula: 1.5 parts of arbuscular mycorrhizal fungi preparation, 0.2 parts of trichoderma harzianum preparation, 5g of xanthan gum, 1.0g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate and 90mg of polylactic acid (the weight is 2500).
The preparation method is the same as example 1.
Comparative example 1
A mycorrhizal fungi microcapsule seed coating agent for improving the soil microbial community structure of saline-alkali soil is prepared by the following formula: 1.2 parts of arbuscular mycorrhizal fungi preparation, 0.2 parts of trichoderma harzianum preparation, 4g of xanthan gum, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution and 0.3g of ethyl lactate.
The preparation method comprises the following steps:
(1) firstly, adding the arbuscular mycorrhizal fungi preparation and the trichoderma harzianum preparation into ethyl acetate, and stirring at 7000r/min for 2min to form a suspension;
(2) adding xanthan gum, calcium carbonate, citric acid aqueous solution and ethyl lactate into the suspension, stirring for 24h at 35-40 ℃ to obtain emulsion, adding sodium chloride aqueous solution with the concentration of 4.5g/L, and stirring for 2min at room temperature at 5000 r/min;
(3) and drying the stirred product in vacuum to obtain the arbuscular mycorrhiza microcapsule seed coating agent.
Comparative example 2
A mycorrhizal fungi microcapsule seed coating agent for improving the soil microbial community structure of saline-alkali soil is prepared by the following formula: 1.4 parts of arbuscular mycorrhizal fungi preparation, 4g of xanthan gum, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate and 100mg of polylactic acid (the weight is 3000).
The preparation method is the same as example 1.
Comparative example 3
A mycorrhizal fungi microcapsule seed coating agent for improving the microbial community structure of saline-alkali soil, and the formula is the same as that in the embodiment 1.
The preparation method comprises the following steps
(1) Firstly, dissolving polylactic acid in 80mL of ethyl acetate to obtain a polylactic acid-ethyl acetate mixed solution, stirring at 7000r/min for 2min, adding the arbuscular mycorrhizal fungi preparation and the Trichoderma harzianum preparation into the mixed solution, and stirring at 7000r/min for 2min to form a primary emulsion;
(2) adding xanthan gum, calcium carbonate, citric acid aqueous solution and ethyl lactate into the primary emulsion, stirring at 35-40 deg.C for 24h to obtain composite emulsion, and stirring at room temperature at 5000r/min for 2 min;
(3) and drying the stirred product in vacuum to obtain the arbuscular mycorrhiza microcapsule seed coating agent.
Comparative example 4
A mycorrhizal fungi microcapsule seed coating agent for improving the microbial community structure of saline-alkali soil, and the formula is the same as that in the embodiment 1.
The preparation method comprises the following steps
(1) Firstly, dissolving polylactic acid in 80mL of ethyl acetate to obtain polylactic acid-ethyl acetate mixed solution, then adding xanthan gum, calcium carbonate, citric acid aqueous solution and ethyl lactate, stirring for 2min at 7000r/min, then adding the arbuscular mycorrhizal fungi preparation and the Trichoderma harzianum preparation, stirring for 24min at 35-40 ℃ at 5000r/min, then adding sodium chloride aqueous solution with the concentration of 4.5g/L, and stirring for 2min at 5000r/min at room temperature;
(3) and drying the stirred product in vacuum to obtain the arbuscular mycorrhiza microcapsule seed coating agent.
Comparative example 5
A mycorrhizal fungi microcapsule seed coating agent for improving the soil microbial community structure of saline-alkali soil is prepared by the following formula: 1.2 parts of arbuscular mycorrhizal fungi preparation, 0.2 parts of trichoderma harzianum preparation, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate and 100mg of polylactic acid (the weight is 3000).
The preparation method comprises the following steps:
(1) firstly, dissolving polylactic acid in 80mL of ethyl acetate to obtain a polylactic acid-ethyl acetate mixed solution, stirring at 7000r/min for 2min, adding the arbuscular mycorrhizal fungi preparation and the Trichoderma harzianum preparation into the mixed solution, and stirring at 7000r/min for 2min to form a primary emulsion;
(2) then adding calcium carbonate, citric acid aqueous solution and ethyl lactate into the primary emulsion, stirring for 24h at 35-40 ℃ to obtain composite emulsion, then adding sodium chloride aqueous solution with the concentration of 4.5g/L, and stirring for 2min at room temperature at 5000 r/min;
(3) and drying the stirred product in vacuum to obtain the arbuscular mycorrhiza microcapsule seed coating agent.
Effects of the embodiment
Firstly, detecting the embedding rate and the particle size of the microcapsules prepared in examples 1-3 and comparative examples 1-5, wherein the embedding rate is measured by an indirect method; detecting the particle size by using a particle size detector; specific results are shown in table 1.
TABLE 1
Secondly, the microcapsules prepared in examples 1 to 3 and comparative examples 1 to 4 are used for seed dressing, the peanut seeds are subjected to seed dressing by a direct coating method, and after the peanut seeds are wetted, the weight ratio of the mixture is determined according to the following formula 1: 300, turning over the seeds until the seeds are uniformly coated on the surfaces of the seeds, then sowing under the same condition, wherein the sowing time of each experimental group is more than 500, harvesting and measuring 35d and 70d after peanut emergence, and measuring mycorrhizal infection frequency, mycorrhizal infection strength and cluster planting abundance, wherein the specific results are shown in table 2.
TABLE 2
And (III) carrying out peanut tests in the Dongying rural high-rise area, wherein the peanut variety is 25, the test field is a light and medium saline-alkali soil with the salt content of 0.3%, the experimental area of each embodiment and comparative example is 1 mu, dressing the prepared seed coating agent, setting a blank control group, carrying out no treatment, counting the peanut incidence rate and the final peanut yield, mainly counting the peanut leaf spot, stem rot, root rot and scab, carrying out other field management and the like according to a common method, and obtaining specific results shown in Table 3.
TABLE 3
And (IV) detecting the volume weight and the alkalization degree of the soil after the flowers are harvested, wherein the initial average alkalization degree of the soil is 34.2%, the volume weight of the soil is measured by adopting a cutting ring method, and specific results are shown in table 4.
TABLE 4
Claims (8)
1. The mycorrhizal fungi microcapsule seed coating agent for improving the soil microbial community structure of saline-alkali soil is characterized by comprising the following raw materials in parts by weight: 1-1.5 parts of arbuscular mycorrhizal fungi preparation, 0.1-0.2 part of trichoderma harzianum preparation, 3-5g of xanthan gum, 1-1.2g of calcium carbonate, 0.1-0.2g of citric acid aqueous solution, 0.2-0.3g of ethyl lactate and 80-100mg of polylactic acid.
2. The mycorrhizal fungi microcapsule seed coating agent as claimed in claim 1, wherein the molecular weight of the polylactic acid is 2000-3000.
3. A process for the preparation of a mycorrhizal fungi microcapsule seed coating according to claim 1 or 2, comprising the steps of:
(1) firstly, dissolving polylactic acid in ethyl acetate to obtain a polylactic acid-ethyl acetate mixed solution, uniformly stirring, adding a arbuscular mycorrhizal fungi preparation and a trichoderma harzianum preparation into the mixed solution, and uniformly stirring to form a primary emulsion;
(2) adding xanthan gum, calcium carbonate, citric acid water solution and ethyl lactate into the primary emulsion, stirring at 35-40 deg.C for 24 hr to obtain composite emulsion, adding sodium chloride water solution, and stirring at room temperature for 2 min;
(3) and drying the stirred product in vacuum to obtain the arbuscular mycorrhiza microcapsule seed coating agent.
4. The preparation method according to claim 3, wherein the feed-liquid ratio of the polylactic acid to the ethyl acetate is 1 mg: 1 mL.
5. The preparation method according to claim 3, wherein the volume ratio of the composite emulsion to the aqueous sodium chloride solution is 1: 5.
6. the method according to claim 5, wherein the concentration of the aqueous sodium chloride solution is 4.5 g/L.
7. The method as claimed in any one of claims 3 to 6, wherein the stirring rate in step (1) is 7000-7500 r/min.
8. The method according to claim 7, wherein in step (2), the stirring rate is 5000-5500 r/min.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911191727.1A CN110746980A (en) | 2019-11-28 | 2019-11-28 | Mycorrhizal fungi microcapsule seed coating agent for improving saline-alkali soil microbial community structure and preparation method thereof |
| NL2025603A NL2025603B1 (en) | 2019-11-28 | 2020-05-18 | Mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil and preparation method thereof |
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| CN201911191727.1A CN110746980A (en) | 2019-11-28 | 2019-11-28 | Mycorrhizal fungi microcapsule seed coating agent for improving saline-alkali soil microbial community structure and preparation method thereof |
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| CN201911191727.1A Pending CN110746980A (en) | 2019-11-28 | 2019-11-28 | Mycorrhizal fungi microcapsule seed coating agent for improving saline-alkali soil microbial community structure and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111826291A (en) * | 2020-07-30 | 2020-10-27 | 左政� | Biological bacterium solution for improving survival rate of trees in arid and saline-alkali areas as well as preparation and application thereof |
| CN112315605A (en) * | 2020-11-03 | 2021-02-05 | 吉林大学 | Implantable bone cyst windowing drainage system |
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| CN1478389A (en) * | 2003-07-14 | 2004-03-03 | 辽宁凤凰蚕药厂 | Application of microcapsule slow-releasing agent in suspensioned seed coating agent |
| CN101341818A (en) * | 2008-08-26 | 2009-01-14 | 新疆农业科学院微生物应用研究所 | Arbuscular mycorrhizal fungi dry powder seed-dressing agent and preparation method thereof |
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2019
- 2019-11-28 CN CN201911191727.1A patent/CN110746980A/en active Pending
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2020
- 2020-05-18 NL NL2025603A patent/NL2025603B1/en not_active IP Right Cessation
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111826291A (en) * | 2020-07-30 | 2020-10-27 | 左政� | Biological bacterium solution for improving survival rate of trees in arid and saline-alkali areas as well as preparation and application thereof |
| CN112315605A (en) * | 2020-11-03 | 2021-02-05 | 吉林大学 | Implantable bone cyst windowing drainage system |
Also Published As
| Publication number | Publication date |
|---|---|
| NL2025603B1 (en) | 2022-12-19 |
| NL2025603A (en) | 2021-07-28 |
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