CN115381934A - Zika病毒疫苗 - Google Patents

Zika病毒疫苗 Download PDF

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CN115381934A
CN115381934A CN202210678575.3A CN202210678575A CN115381934A CN 115381934 A CN115381934 A CN 115381934A CN 202210678575 A CN202210678575 A CN 202210678575A CN 115381934 A CN115381934 A CN 115381934A
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J·巴尔贝罗卡尔扎多
M·内本菲尔
R·施勒格
M·韦伯
J·费鲁施
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Abstract

本发明涉及Zika病毒疫苗。具体地，本文描述了Zika病毒疫苗和组合物以及生产所述疫苗和将其施用于有需要的受试者的方法。

Description

Zika病毒疫苗
本申请是申请号为201680074939.X，申请日为2016年12月23日，发明名称 为“Zika病毒疫苗”的中国专利申请的分案申请。
技术领域
本公开涉及用于纯化用于疫苗的Zika病毒的方法，具体涉及改进的蔗糖梯度 处理步骤，其允许分离诸如硫酸鱼精蛋白的杂质。本公开还涉及Zika病毒疫苗和 组合物以及用于生产所述疫苗和将疫苗施用于受试者以产生抗Zika病毒免疫应答 的方法。
背景技术
硫酸鱼精蛋白的不良反应已经已知多年。先前暴露于鱼精蛋白能够诱发体液 免疫反应，并使易感个体易于发生随后使用该药物的不良反应。通过使用含鱼精 蛋白的胰岛素或在肝素中和过程中暴露于鱼精蛋白的患者在静脉内接受大剂量鱼 精蛋白后可能会发生危及生命的反应和致命的过敏反应。在没有皮下注射含鱼精 蛋白的胰岛素的局部或全身性过敏反应的情况下，能够发生对静脉内鱼精蛋白的 严重反应。虽然没有明确的证据表明与接种疫苗有关的硫酸鱼精蛋白过敏反应， 但含有鱼精蛋白杂质的疫苗在其标签中有一项预防和禁忌警告，表明在先前剂量 的含鱼精蛋白疫苗后的严重过敏反应(如
Figure BDA0003695595590000011
参见疾病预防控制中心网站 http://www.cdc.gov/japaneseencephalitis/vaccine/)是进一步剂量的禁忌症。因此消 除所述杂质是提高安全性的医学要求。另一方面，硫酸鱼精蛋白是一种极好的工 具(通常比其它工具如benzonase更好)以纯化在细胞基质上生长的病毒的粗收获 物。
自从1947年在乌干达发现于猕猴以来，在2007年第一次在亚洲和非洲的流 行地区以外发现了Zika病毒。从那时起，该病毒在法属波利尼西亚引起了大规模 流行，到2015年在太平洋岛屿和南美和中美洲传播(世界卫生组织“Zika情况报 告”，2016年2月5日)。有证据表明，除由伊蚊种类蚊子传播外，还可能存在其 它载体，并且可通过输血、经胎盘传播和通过性传播来传播病毒(世卫组织Zika病毒情况说明，2016年2月)。尽管Zika病毒感染的症状包括轻度发热、皮疹和 结膜炎，但感染与神经系统疾病之间可能存在相关性，包括妊娠期感染后的胎儿/ 新生儿中的吉兰-巴雷综合征和小头畸形。目前没有针对Zika病毒的特定治疗或疫 苗，唯一预防措施是控制蚊子载体。由于伊蚊的广泛流通，多种传播途径以及潜 在的严重的感染神经系统影响，Zika病毒对公共卫生构成严重威胁。
针对Zika病毒的预防性疫苗是流行地区和传播媒介地理区域的迫切医疗需 求。此外，由于Zika病毒感染对胚胎和胎儿发育具有严重后果，因此需要一种安 全有效的疫苗用于生育潜能女性或怀孕妇女。怀孕期间的施用疫苗对母亲和发育 中的胎儿都必须非常安全。尽管减毒活病毒疫苗非常有效，但通常认为它们施用 于怀孕妇女不足够安全。在这方面，在接种疫苗的受试者中缺乏繁殖能力的灭活 病毒疫苗被认为更安全。开发灭活的Zika病毒疫苗以施用于有风险的患者可以满 足这种需求。
发明内容
在病毒纯化过程中，观察到如所预期的那样，在细胞基质上产生的病毒收获 物中加入硫酸鱼精蛋白不仅去除了源自宿主细胞的DNA，而且还实际上从制剂中 消除了未成熟的和非感染性的病毒颗粒。这一发现为获得高纯度的感染性病毒颗 粒以用于诸如疫苗制剂的应用提供了简化、温和、可重复和广泛适用的过程。另 外，令人惊讶地发现，所述硫酸鱼精蛋白能够非常有效地从病毒级分中分离，允 许以高产率生产更安全的疫苗。
本文公开了包含灭活的Zika病毒的病毒疫苗和组合物，以及生产所述疫苗和 组合物的相关方法。还提供了施用Zika病毒疫苗以在受试者中预防Zika病毒感染 和/或产生抗病毒免疫应答的方法，例如有风险暴露于Zika病毒的受试者。特别地， 本发明涉及包含最佳灭活的Zika病毒颗粒的病毒疫苗，其中适当剂量的Zika病毒 颗粒能够以至少70％的可能性，优选80％的可能性使施用病毒疫苗的受试者血清 转化。本发明的另一个优点是生产所述疫苗和组合物的相关方法是非常有效的并 提供很大程度上不含杂质特别是硫酸鱼精蛋白的纯组合物，从而允许大量生产疫 苗。针对Zika病毒提供了上述的详细实验示例。
本文公开的关于本发明的灭活Zika病毒疫苗的免疫原性的体内数据表明，该 病毒具有惊人的有效免疫原性并且还具有高度的交叉保护性(在非洲和亚洲菌株 中非常相似的免疫原性)。数据表明，免疫原性出乎意料地高于最近报道的灭活Zika病毒候选疫苗(Larocca等，2016，Nature doi：10.1038/nature18952.)。灭活 病毒是最安全的疫苗之一，尤其优选用于向安全性特别关注的人群(例如孕妇、 儿童和免疫受损个体)递送，这使得本文公开的灭活Zika病毒特别合适。获得高 滴度的灭活病毒是该领域的挑战。本文公开的用于纯化灭活的Zika病毒的方法不 仅产生高产量，而且产生非常纯的药物物质。
本发明的每个限制能够包含本发明的各种实施方式。因此，预计涉及任何一 个要素或要素组合的本发明的每个限制能够包括在本发明的每个方面中。本发明 的应用不限于以下说明书中阐述的或附图中所示的组件的构造和布置的细节。本 发明能够具有其它实施方式并且能够以各种方式实践或执行。
附图说明
不意在按比例绘制附图。这些图仅是说明性的，并不是实现本公开所必需的。 为了清楚起见，并非每个组件都可以在每个图中标记，使用多重比对包Jalview (Waterhouse等，2009，Bioinformatics 25(9)1189-1191)进行比对。在附图中：
图1：平均距离树(以％同一性，nt)，完整的基因组。
图2：相邻连接树(以％同一性，nt)，完整的基因组。
图3：成对比对-Jalview(％同一性，nt)，完整的基因组。
图4：平均距离树(以％同一性，aa)，E蛋白。
图5：相邻连接树(以％同一性，aa)，E蛋白。
图6：成对比对-Jalview(％同一性，aa)，E蛋白。
图7：比对(阴影：％同一性，aa)，E蛋白。
图8：宿主细胞中病毒颗粒成熟的示例。如在黄病毒中所观察到的，颗粒的完 全成熟需要通过宿主蛋白酶弗林蛋白酶对前体膜糖蛋白(prM)进行蛋白水解切割。 并非所有的prM分子都被切割，导致从细胞中释放成熟的、嵌合的或未成熟样的 构象。嵌合和未成熟形式通常不具感染性，只有成熟病毒颗粒具有感染性并具有 血凝素(HA)/TCID50活性。(图改编自Plevka等，黄病毒的成熟起始于一个或 多个二十面体独立的成核中心，EMBO reports(2011)12,602-606)。
图9A至9B：从粗收获物到药物物质(疫苗)制剂的示例性下游病毒纯化方 法，即本发明方法(图9A)的优选实施方式。(图9B)中示出了示例性病毒灭活 过程的流程图。
图10：选择性除去Zika病毒聚集体以及Vero HCP和LMW杂质(30×浓缩 Zika病毒收获第5天的SEC-HPLC)的PS处理结果。
图11：在不同时间点的PS处理之前的单独的30×浓缩Zika收获物的 SEC-HPLC。
图12：在不同时间点的PS处理之后的单独的30×浓缩Zika收获物的 SEC-HPLC。较小的图表显示随时间观察到的细胞病变效应(CPE)。
图13：制备蔗糖梯度。
图14：显示了来自Zika纯化的蔗糖梯度收获物的代表性SDS-PAGE。
图15：JEV和ZikaV收获物时间表/收益的比较。
图16：ZikaV NIV的SEC-HPLC洗脱曲线。数据在Dionex Ultimate 3000/ Superose6Increase柱上处理。两个图都来自相同的色谱图。上图是完整的洗脱图； 下图是ZIKAV洗脱峰的放大图。
图17：灭活ZikaV的SEC-MALLS分析。
图18：Zika NIV的累积粒度分布。
图19：用汇集的小鼠血清中和Zika病毒H/PF/2013的图示。无血清斑块的数 量设定为100％。使用3-参数法计算EC50。
图20：用汇集的小鼠血清中和Zika病毒MR766的图示。无血清斑块的数量 设定为100％。使用3-参数法计算EC50。
图21：通过ELISA和SEC-HPLC(Dionex Ultimate 3000，Superose 6柱)分 析的中和灭活病毒(NIV)中的JEV抗原含量之间的相关性。
具体实施方式
本文公开了包含灭活的Zika病毒的Zika病毒疫苗和组合物，以及生产所述疫 苗和组合物的相关方法。还提供了施用所述病毒疫苗以在受试者中预防病毒感染 和/或产生抗病毒免疫应答的方法，例如有风险暴露于病毒的受试者。具体而言， 本发明涉及包含最佳灭活的病毒颗粒的病毒疫苗，其中适当剂量的病毒颗粒能够 以至少70％的可能性，优选80％的可能性使施用病毒疫苗的受试者血清转化。本 发明的另一个优点是生产所述疫苗和组合物的相关方法是非常有效的，并提供很 大程度上不含杂质特别是硫酸鱼精蛋白的纯组合物，从而允许大量生产疫苗。针 对Zika病毒提供了上述示例。
本文公开了纯化来自粗制剂的Zika病毒颗粒的下游方法。下游方法能够应用 于未适应在特定细胞底物上繁殖的病毒或应用于部分/完全细胞底物适应的Zika病 毒颗粒。
本发明的方面提供用于纯化感染性Zika病毒颗粒的方法，其包括以下步骤： (a)提供包含病毒颗粒和杂质的粗收获物(a)，其中所述杂质通过在细胞基质上 培养所述病毒颗粒而产生；(b)通过用包含鱼精蛋白盐，优选硫酸鱼精蛋白的试 剂沉淀来减少粗收获物(a)中的杂质以获得病毒制剂(b)；以及通过优化蔗糖密 度梯度离心进一步纯化病毒制剂(b)以获得包含感染性病毒颗粒的病毒制剂(c)。
在一些实施方式中，步骤(b)中的硫酸鱼精蛋白的浓度为约1至10mg/ml， 更优选约1至5mg/ml，更优选约1至2mg/ml。在一个实施方式中，步骤(b)中 的硫酸鱼精蛋白的浓度为约2mg/mL。在一个实施方式中，硫酸鱼精蛋白的浓度为 1.2至1.8mg/ml，更优选1.4至1.6mg/ml。在一个优选实施方式中，步骤(b)中 的硫酸鱼精蛋白的浓度为约2mg/ml。
在一些实施方式中，病毒制剂(c)的残余宿主细胞DNA小于1μg/mL，特别 是小于900、800、700、600、500、400、300或200ng/mL，优选小于100ng/mL。 在一个优选实施方式中，病毒制剂(c)的残余宿主细胞DNA小于10ng/mL。在 一些实施方式中，最终病毒制剂(c)的残余宿主细胞蛋白小于10μg/mL，特别是 小于9、8、7、6、5、4、3或2μg/mL，优选小于1μg/mL。在一个优选实施方式 中，病毒制剂(c)的残余宿主细胞蛋白小于100ng/mL。在一些实施方式中，最终 病毒制剂(c)的残余非感染性病毒颗粒小于10μg/mL，特别是小于9、8、7、6、 5、4、3或2μg/mL，优选地小于1μg/mL。在一个优选实施方式中，病毒制剂(c) 的残余非感染性病毒颗粒的含量小于100ng/mL。
在一些实施方式中，残余鱼精蛋白小于1μg/mL，特别是小于900、800、700、 600、500、400、300或200ng/mL，优选小于100ng/mL，更优选小于HPLC的检 测限，特别是低于最终药物的检测限。在一些实施方式中，通过HPLC或尺寸排 阻色谱法(SEC)测试PS含量。例如，HPLC在JEV蔗糖梯度池样品中作为常规 释放测定进行PS测定是有效的并且非常灵敏(即，LOQ 3μg/mL；LOD 1μg/mL)。 在本发明中，Zika病毒DS样品中的PS含量<LOD。在一个实施方式中，PS含量 的HPLC评估可以使用30％乙腈、0.1％三氟乙酸作为溶剂，以0.6ml/min的流速在 25℃下并在214nm处检测，而在Superdex Peptide 10/300GL柱(GE：17-5176-01) 上进行。在纯化病毒制剂中测量残余鱼精蛋白的更灵敏方法是质谱法(MS)。在 一些实施方式中，Zika病毒制剂中的残余PS水平通过MS或其它此类高灵敏度的 方法例如核磁共振(NMR)来测试。用这种方法，残余PS以及PS的片段和/或分 解产物可以以痕量进行检测，例如水平低至例如106、107或108个分子/典型样品 负载。在一些实施方式中，在蔗糖梯度池中测试PS水平。在一些实施方式中，在 药物产品中测试PS水平。在一些实施方式中，在药物物质中测试PS水平。
在一些实施方式中，包含病毒颗粒和杂质的粗收获物(a)在步骤(b)之前 进行一个或多个预纯化步骤。在一些实施方式中，一个或多个预纯化步骤包括通 过酶处理在包含病毒颗粒和杂质的粗收获物(a)中消化宿主细胞基因组DNA。在 一些实施方式中，一个或多个预纯化步骤包括过滤、超滤、浓缩、缓冲液交换和/ 或渗滤。在一些实施方式中，一个或多个预纯化步骤是使用孔径等于或小于1μm 的过滤器进行过滤。在一些实施方式中，过滤器具有等于或小于0.2μm的孔径。 在一个优选实施方式中，过滤器具有0.2μm的孔径。在一些实施方式中，通过切 向流过滤(TFF)进行浓缩和/或超滤/渗滤和/或缓冲液交换。在一些实施方式中， 使用截留值等于或小于300kDa的中空纤维膜对包含病毒颗粒和杂质的粗收获物 (a)进行超滤/渗滤。在一个优选实施方式中，中空纤维膜具有约100kDa的截留 值。
根据本发明的方法还可以包括使用蔗糖梯度，优选优化的蔗糖梯度。蔗糖梯 度优选优化用于去除硫酸鱼精蛋白，也用于去除未成熟的病毒颗粒或未感染的病 毒颗粒或宿主细胞蛋白或核酸(DNA、RNA、mRNA等)或其它宿主细胞碎片。 在本发明中，优化蔗糖梯度包含至少两个、至少三个、至少四个具有不同密度的 蔗糖溶液层。在一个实施方式中，待纯化的病毒制剂提供于具有约8％、约9％、 约10％、约11％、约12％蔗糖密度(w/w)的蔗糖溶液中，优选约10％。在一个 实施方式中，一种梯度的蔗糖溶液具有约45％、约46％、约47％、约48％、约49％、 约50％、约51％、约52％、约53％、约54％、约55％蔗糖密度(w/w)，优选约50％。在一个实施方式中，一种梯度的蔗糖溶液具有约30％、约31％、约32％、 约33％、约34％、约35％、约36％、约37％、约38％、约39％、约40％蔗糖密度 (w/w)，优选约35％。在一个实施方式中，一种梯度的蔗糖溶液具有约10％、约11％、约12％、约13％、约14％、约15％、约16％、约17％、约18％、约19％、 约20％蔗糖密度(w/w)，优选约15％。在一个优选的实施方式中，蔗糖梯度包含 约50％、约35％和约15％(w/w)蔗糖的三个蔗糖溶液层，待纯化的病毒组合物 包含在约10％(w/w)蔗糖中。因为本发明提供了不仅测试宿主细胞DNA而且测 试未成熟病毒颗粒的手段，本领域技术人员能够更精确地优化蔗糖梯度以进行最 有效的纯化，并且包括另外的工具，例如PRNT测定以监测纯化成功。
在一些实施方式中，病毒颗粒是活病毒、嵌合病毒、减毒活病毒、修饰的活 病毒或重组活病毒。在进一步的步骤中，本发明的病毒颗粒可以任选地灭活。在 一些实施方式中，病毒颗粒是病毒颗粒的减毒形式。例如，与野生型病毒相比， 病毒可能具有降低的宿主感染性、毒力和/或复制。在一些实施方式中，病毒是突 变或修饰的病毒，例如病毒的核酸可以含有相对于野生型病毒至少一个突变。在 一些实施方式中，病毒是重组活病毒，意指重组产生的病毒，并且可含有来自不 同来源的核酸。
在一些实施方式中，Zika病毒颗粒是活病毒、减毒活病毒、修饰的活病毒或 重组活病毒。在优选的实施方式中，Zika病毒是来自亚洲谱系的Zika病毒。
在一些实施方式中，最终病毒制剂的杂质的相对减少量相对于包含病毒颗粒 和杂质的液体培养基(a)在60至95％的范围内。在一些实施方式中，最终病毒 制剂的残余杂质小于1％。
在一些实施方式中，Zika病毒在选自以下的细胞系中增殖：EB66细胞系、Vero 细胞系、Vero-αHis细胞系、HeLa细胞系、HeLa-S3细胞系、293细胞系、PC12 细胞系、CHO细胞系、3T3细胞系、PerC6细胞系、MDSK细胞系、鸡胚胎成纤 维细胞系、鸭细胞系和二倍体禽细胞系。在一些实施方式中，所述细胞系是鸭细 胞系。在一些实施方式中，所述细胞系是二倍体禽类细胞系。在一些实施方式中， 所述细胞系是EB66细胞系。在一个优选的实施方式中，所述细胞系是Vero细胞 系。
本发明的各方面提供了本文所述的任何方法在制造抗病毒感染免疫的组合物 中的用途。在一个优选实施方式中，组合物或疫苗针对Zika病毒，例如亚洲谱系 的Zika病毒。
其它方面提供了包含通过本文所述的任何方法可获得的用于治疗和/或预防 (即保护)病毒感染的Zika病毒颗粒的组合物。在一个优选实施方式中，病毒感 染是由Zika病毒引起的，例如亚洲谱系的Zika病毒。
此外，本文公开了包含灭活的Zika病毒的Zika病毒疫苗和组合物，以及生产 所述疫苗和组合物的相关方法。还提供了施用Zika病毒疫苗以在受试者中预防 Zika病毒感染和/或产生抗Zika病毒免疫应答的方法，例如有风险暴露于Zika病 毒的受试者。
Zika病毒是一种与登革热病毒密切相关的黄病毒，并且类似地被伊蚊种类蚊 子传播，尽管Zika病毒的其它节肢动物载体也是可能的。自从它于1947年首次从 乌干达Zika森林的猕猴中分离出来以后，很少有人报道过人类感染的事件，特别 是在非洲和亚洲流行地区之外，直到2007年法属波利尼西亚发生大规模爆发 (Haddow等，PLoS NeglectedTropical Diseases(2012)6(2)，Malone等，PLoS Neglected Tropical Diseases(2016)10(3))。此后，该病毒在太平洋岛屿(包括大洋 洲)和南美和中美洲中传播(世界卫生组织“Zika情况报告”，2016年2月5日)。
除了被感染的蚊子叮咬传播之外，证据还表明个体之间可能会发生传播，例 如受感染个体的血液，从感染的母体到胎儿的经子宫/胎盘传播，性伴侣之间的性 传播，并可能通过其它局部传输路线。孕期Zika病毒感染与胎儿/新生儿小头畸形 之间可能存在关联。小头畸形是一种罕见病症，婴儿的头围显着低于基于年龄、 性别和种族的平均值所预期的。这是大脑未能进行适当的胚胎发育的结果，并且 90％的病例与智力低下有关(Rocha等(2016)Bull World Health Organ，2016年2 月8日)。
已经患有Zika病毒感染的个体与吉兰-巴雷综合征的发病率之间可能存在关 联，吉兰-巴雷综合征是一种神经疾病，其中个体的免疫系统破坏周围神经系统轴 突周围的髓鞘(世界卫生组织“Zika情况报告”，2016年2月5日)。
目前还没有针对Zika病毒的特定治疗或疫苗，目前唯一的防止感染的措施是 通过病媒控制并避免前往发生爆发的地区。
本文描述的是包含灭活的Zika病毒的Zika病毒疫苗和组合物，其提供了可以 帮助预防Zika病毒感染的安全方法，用于产生对Zika病毒的免疫应答，包括病毒 中和抗体。
任何Zika病毒株均可用于本文所述的方法和组合物中。在一些实施方式中， Zika病毒是在Zika病毒爆发期间来自受感染受试者的分离物。在一些实施方式中， Zika病毒是从非洲或非洲病毒谱系分离的菌株。在一些实施方式中，Zika病毒是 从亚洲或亚洲谱系分离的菌株(还包括来自法属波利尼西亚的菌株)。在一些实施 方式中，Zika病毒是从美洲(南美洲、中美洲或北美)分离的菌株，例如苏里南 Zika病毒株。在一些实施方式中，Zika病毒具有相对应于(但不限于)由GenBank 登录号AY632535.2、KU321639.1、KU497555.1、KU501215.1、KU509998.1、 KU527068.1、KU681081.3、KU681082.3、KU707826.1，KU744693.1或LC002520.1 提供的DNA序列的RNA基因组，或本文部分或完全披露的RNA基因组(SEQ IDNO：2至69)。
在一些实施方式中，本发明的方法导致来自包含感染性Zika病毒颗粒和非感 染性病毒颗粒和其它病毒产物的粗收获物的感染性Zika病毒颗粒的富集，使得感 染性病毒颗粒的富集相对于包含病毒颗粒和杂质的粗收获物(a)的总病毒颗粒含 量为至少50％、至少60％、至少70％、至少80％、至少90％、至少95％、优选至 少80％、尤其至少85％。
在一些实施方式中，相对于粗收获物中的所有杂质，最终病毒制剂的残余杂 质小于10％、9％、8％、7％、6％、5％、4％、3％、2％、1％，优选小于5％，如通 过SEC-HPLC(尺寸排除色谱-HPLC)测定的。
本发明的一个独特方面是认识到，可以采用用于日本脑炎疫苗(JEV)的疫苗 设计和纯化方法有关的技术知识(参见Srivastava AK等人，2001，Vaccine 19,4557-4565，WO99/11762)并加以改进以加速开发Zika病毒疫苗并尽快将其提 供给有需要的受试者。针对
Figure BDA0003695595590000081
公开的工业过程提供了针对JEV的非常有效 的疫苗，其补充了本文公开的进一步显著改进以提供相比于可用的JEV疫苗更有 效(更高产量)和更安全(具有过敏潜力的较少或不具有硫酸鱼精蛋白)的Zika 疫苗。本文公开的疫苗的特别创新是它们通过发展改善的蔗糖梯度而促进的最终 药物物质中大大降低的鱼精蛋白盐(SEQ ID NO：1)含量。所述蔗糖梯度不仅允 许分离硫酸鱼精蛋白，而且还允许通过甲醛进行非常有效的灭活，并且导致Zika 具有超过90％的产率(采用本文公开的改进方法)，与公开的JEV方法的产率约为35％的情况，参见实验部分进行比较。
本公开的方面涉及在Vero组织培养细胞中产生病毒的方法。Vero细胞是源 自非洲绿猴肾脏的常用组织培养细胞系。本文所述方法中使用的Vero细胞是Vero (WHO)细胞系，其从健康保护机构总细胞集合获得，目录号为88020401。
Vero细胞可以生长至融合单层，例如在组织培养瓶中；在悬浮液中(在微载 体上)，例如在滚瓶中；或用于病毒生产的任何其它细胞培养系统中。在一些实施 方式中，Vero细胞在生物反应器中生长以用于病毒生产。对于斑块测定或斑块减 少中和测试(PRNT)，Vero细胞在组织培养瓶、培养皿或板的孔中以单层生长。 为了用病毒感染Vero细胞，用病毒接种培养基并将细胞与病毒一起温育一段时间。 接种后可以洗涤细胞以去除在给定的时间内没有吸附到细胞上的任何病毒。
本文提供的方法涉及在Vero细胞中传代病毒。如本文所用，术语“传代”是 指用病毒感染Vero细胞群体，随后用感染第一Vero细胞群体产生的病毒接种第二 Vero细胞群体。在一些实施方式中，将来自感染的Vero细胞的培养基的一部分(含 有从感染细胞释放的病毒)用于接种第二Vero细胞群体。这被称为一次传代或一 轮传代。传代可以连续进行，例如，将来自感染的第二Vero细胞群体的培养基的 一部分用于接种第三Vero细胞群体，等等。在一些实施方式中，将从单个斑块获 得的病毒用于接种另一细胞群体。
在一些实施方式中，病毒在Vero细胞中传代若干次，例如至少4、5、6、7、 8、9、10、11、12、13、14、15、16、17、18、19、20、21、22、23、24、25、 26、27、28、29、30、31、32、33、34、35、36、37、38、39或40次。在一些实 施方式中，病毒在Vero细胞中传代至少4次或5次。在一些实施方式中，病毒在 Vero细胞中传代至少30次。重要的是病毒群体(即病毒序列)在所述传代中尽可 能保持恒定。如果发生病毒适应(即在原始病毒群中出现突变病毒)，则优选所述传代不用于制造所述病毒的情况下，例如对于Zika，发现直到第3代和培养到第7 天都可以使用，没有病毒种群的重大变化，即引入具有突变的病毒种群。然而， 这种观察需要针对每种病毒株进行并且可能不同。
在一些实施方式中，Vero细胞在接种例如通常0.01MOI(感染复数)的病毒 后温育至少2天，以允许在传代之前进行病毒生产。在一些实施方式中，将Vero 细胞在传代前的接种病毒后温育至少3、4、5、6、7、8、9、10或超过10天，例 如至少7天。Vero细胞在病毒接种后培养的天数取决于诸如用于接种细胞的感染 多样性和培养基中所需的病毒滴度的因素。病毒在Vero细胞中的连续传代可能导 致产生适应Vero细胞的病毒株。
可以收获(收集)来自感染的Vero细胞的培养基以获得病毒。在一些实施方 式中，从感染的Vero细胞收获培养基并用新鲜培养基代替，然后在另一段时间后 收获。在一些实施方式中，从感染的Vero细胞收获的培养基汇集自独立的Vero细 胞培养物和/或独立的天数。例如，感染后7或9天可以重复收获4次，并且每单 位细胞培养物产生高的病毒产量。为了最小化Zika病毒株对Vero细胞的适应性， 发现Vero细胞可以在传代前温育至少7天，更优选5天，随后可以在第2、3、5 和7天或第2、3和5天收获上清液(另见实验部分)。收获的培养基可以保存在 +4℃，然后从培养基中纯化病毒长达2周。
在一些实施方式中，来自感染和裂解的Vero细胞的碎片可以从收获的培养基 中去除，称为培养基的“澄清”。收获的培养基可以通过本领域已知的常规方法澄 清，例如低速离心，例如在1500g下10分钟，和/或通过孔径0.45μm的过滤器过 滤。收获的培养基可以在浓缩前储存在+4℃。
为了将Zika病毒的滴度浓缩在收获的培养基中，可以通过本领域已知的任何 方法对其进行浓缩。例如，可以通过包括但不限于超滤、超速离心、离心浓缩器、 真空离心和冻干的方法来浓缩收获的培养基。在一些实施方式中，通过超滤浓缩 收获的培养基并收集含有Zika病毒的保留物。在一些实施方式中，通过沉淀浓缩 收获的培养基，其中将聚乙二醇(PEG)8000溶解在培养基中(至多10％)，并将 沉淀物溶解在缓冲液例如磷酸盐缓冲盐水(PBS，pH 7.0)中。
可以将收获的培养基沉淀以产生病毒上清液。在一些实施方式中，将收获的 培养基沉淀以从收获的培养基中去除Vero细胞DNA和其它不需要的物质，例如 Vero细胞碎片。在一些实施方式中，收获的培养基在沉淀之前浓缩。在一些实施 方式中，通过将硫酸鱼精蛋白(例如SEQ ID NO：1)加入到收获的培养基中并且 例如在+4℃或在冰上温育混合物来沉淀收获的培养基。在一些实施方式中，用 benzonase处理收获的培养基以从收获的培养基中去除Vero细胞DNA和其它不需 要的物质，例如Vero细胞碎片。然而，发现硫酸鱼精蛋白处理是优选的(参见实 验部分)。在一些实施方式中，将沉淀的培养基离心以收集沉淀的物质，并收集称 为“病毒上清液”的含有病毒的上清液。
沉淀后可以进一步纯化病毒上清液，例如密度梯度超速离心。在一些实施方 式中，通过蔗糖梯度进一步纯化病毒上清液。可以从蔗糖梯度收集级分并分析病 毒的存在。分析病毒阳性级分的方法包括斑块测定、血凝测定、聚丙烯酰胺凝胶 电泳和抗原测定(如Western印迹和ELISA)。根据病毒的滴度和其它杂质的含量， 可以汇集含有病毒的级分。存在于病毒上清液中的杂质水平或量可以通过测试 Vero细胞DNA、病毒聚集体和/或Vero细胞蛋白来估计(参见实验部分)。本发明 的具体实施方式是改进的蔗糖梯度，其允许如实验部分所示的有效的鱼精蛋白分 离。令人惊讶地发现，将具有10％(w/w)蔗糖的含病毒级分添加到简单三层的蔗 糖密度梯度(例如包含15％(w/w)蔗糖溶液，35％(w/w)蔗糖溶液和50％(w/w) 蔗糖溶液)导致有效分离硫酸鱼精蛋白，而没有很多病毒损失。因此，本发明的一个特别优选的实施方式是使用能够有效地分离硫酸鱼精蛋白的蔗糖密度梯度， 其中所述蔗糖密度梯度用于纯化病毒，例如本文所述的病毒，即Zika病毒。
为了获得用于对受试者施用的安全疫苗或组合物，病毒上清液可以是灭活的 (参见Zika病毒的实验部分)。如本文所用，术语“灭活的”和“最佳灭活的”可 以互换使用，指的是使病毒不能感染宿主细胞(非感染性)的过程(或其结果)， 但不影响或基本上不影响病毒的抗原性，例如暴露于病毒表面的免疫原性抗原能 够刺激受试者的免疫应答(例如抗原特异性抗体)。所谓“不影响或基本上不影响 病毒的抗原性”是指灭活的病毒保留至少50％、至少55％、至少60％、至少65％、 至少70％、至少75％、至少80％、至少85％、至少90％、至少95％、至少96％、 至少97％、至少98％、至少99％、或甚至基本上100％的未进行灭活的病毒的抗原 性。
本领域已知多种用于灭活病毒的方法。在一些实施方式中，通过化学灭活、 热灭活、pH灭活或UV灭活使病毒灭活。
在一些实施方式中，灭活是通过化学灭活并且包括使病毒与一种或多种化学 灭活剂在使病毒灭活但抗原性表位基本上完整的条件下接触一段时间。在一些实 施方式中，病毒灭活的时间长于完全灭活病毒所需的时间。在一些实施方式中， 将病毒上清液灭活病毒灭活所需的天数加上至少一天的额外天数。病毒上清液的 样品可以在整个灭活过程中进行一次或多次，并通过本领域已知的任何方法，例 如通过感染单层宿主细胞(即斑块试验)来评估病毒活力(感染力)。使用这样的 程序，可以确定完全灭活病毒所需的时间段，并且选择较长的时间段以确保完全 灭活。
在一些实施方式中，使病毒与化学灭活剂接触1天至50天、2天至40天、2 天至30天、2天至20天、2天至10天、3天至9天、4天至8天、5天至7天、2 天至5天、或5天至10天。在一些实施方式中，病毒与一种或多种化学灭活剂接 触至少1天、2天、3天、4天、5天、6天、7天、8天、9天、10天、11天、12 天、13天、14天、15天、16天、17天、18天、19天、20天、21天、22天、23天、24天、25天、26天、27天、28天、29天、30天、31天、32天、33天、34 天、35天、36天、37天、38天、39天、40天、41天、42天、43天、44天、45 天、46天、47天、48天、49天或至少50天。
在一些实施方式中，化学灭活在约+5℃、+10℃、+15℃、+20℃、+25℃、+30℃、 +35℃、+40℃或约+45℃下进行。在一些实施方式中，化学灭活在约+4℃下进行。 在一些实施方式中，化学灭活在约+22℃下进行。
本领域已知的任何化学灭活剂可适用于在本文所述的方法中灭活病毒。本领 域技术人员将会理解，诸如化学灭活剂和进行灭活的温度的因素可能影响使病毒 完全灭活所需的时间长度(天数)。化学灭活剂的实例包括但不限于甲醛、酶、β- 丙内酯、乙醇、三氟乙酸、乙腈、漂白剂、脲、盐酸胍、磷酸三正丁酯、亚乙基 亚胺或其衍生物、以及有机溶剂如吐温、Triton、脱氧胆酸钠和磺基甜菜碱。优选 的灭活是用甲醛在22℃±2℃下灭活约10天。
在一些实施方式中，灭活剂在病毒化学灭活后被中和。在一些实施方式中， 灭活剂是甲醛并且在使用硫代硫酸钠或偏亚硫酸氢钠进行化学灭活后被中和。
在一些实施方式中，通过热灭活使病毒灭活。在一些实施方式中，热灭活包 括将病毒暴露于加热(例如干燥加热或蒸气加热)一段时间。在一些实施方式中， 热灭活涉及将病毒暴露于约+40℃、+45℃、+50℃、+55℃、+60℃、+65℃、+70℃、 +75℃、+80℃、+85℃、+90℃、+95℃或约+100℃。在一些实施方式中，将病毒暴 露于加热至少5小时、6小时、7小时、8小时、9小时、10小时、11小时、12小 时，13小时、14小时、15小时、16小时、17小时、18小时、19小时、20小时， 21小时、22小时、23小时、24小时、36小时、48小时、60小时、72小时、84 小时、96小时或更长。优选的热灭活包括将病毒暴露于约+56℃的温度60分钟。
在一些实施方式中，通过将病毒暴露于酸性或碱性条件一段时间以使病毒完 全灭活来使病毒灭活。可以将病毒制剂的pH调节至所需的pH，例如通过向病毒 制剂中加入酸、碱或具有特定pH的缓冲剂。在一些实施方式中，病毒在约2、2.5、 3、3.5、4、4.5、5或约5.5的酸性pH下灭活。在其它实施方式中，病毒在约8、 8.5、9、9.5、10或约10.5的碱性pH下灭活。
在一些实施方式中，使用UV灭活使病毒灭活。UV灭活包括将病毒暴露于高 能辐射(例如UV-A、UV-B或UV-C)一段时间。应该理解的是，任何两种或更多 种灭活方法可以被组合并且同时或连续地执行。
可以随后透析灭活的病毒以除去任何不需要的物质，包括灭活剂和任何中和 剂，和/或用药学上可接受的缓冲液代替缓冲剂以施用于受试者。在一些实施方式 中，灭活的病毒用PBS透析。另外或可选地，灭活的病毒可以通过0.22μm过滤器 过滤，例如无菌过滤。
本文所述的任何方法或用途可以用于预防受试者中的Zika病毒感染。如本文 所用，术语“预防”、“防止”和“保护”包括向受试者施用病毒疫苗或组合物以 减少或延迟临床或亚临床症状表现、并发症、病理的发作或者疾病或感染的生化 标志，或减少或抑制Zika病毒的扩散/传播。如本文所用，可预防性地(例如在感 染之前)施用于受试者的抗原(例如灭活Zika病毒)可以被称为疫苗。
Zika疫苗
如本文所述，Zika病毒在感染受试者后可引起多种症状中的任一种，并且通 常特征在于轻度发热；面部、颈部、躯干、上臂的皮疹(皮疹)；头痛；对光线敏 感；非炎性关节痛；结膜炎；食欲不振；腹泻；腹痛；和/或头晕。孕期Zika病毒 感染与胎儿/新生儿小头畸形相关。吉兰-巴雷综合征的发作或其症状之间也有可能 存在关联。诊断暴露于Zika病毒或疑似暴露于Zika病毒的受试者的Zika病毒感 染包括检测病毒特异性抗体的存在和/或分子检测，例如PCR或Zika病毒的实时 PCR检测。
本文提供用于向有需要的受试者施用治疗有效量的剂量的Zika病毒疫苗的方法。在一些实施方式中，所述受试者是哺乳动物受试者，例如人、非人灵长类动 物、啮齿动物、兔子、绵羊、狗、猫、马或牛。在一些实施方式中，所述受试者 是小鼠。在一些实施方式中，受试者是人受试者，例如儿童、成人或老年人。在 一些实施方式中，受试者是女性受试者。在一些实施方式中，受试者是孕妇或计 划成为怀孕。在一些实施方式中，受试者有风险暴露于Zika病毒。在一些实施方 式中，受试者住在或前往Zika病毒存在或被认为存在的区域。在一些实施方式中， 受试者先前已经感染过或接种过登革热病毒疫苗；即具有抗体依赖性疾病增强的 风险。在一些实施方式中，受试者住在或前往正在经历Zika病毒感染暴发的区域。 在一些实施方式中，受试者住在或前往能够传播Zika病毒载体的节肢动物载体存在或被认为存在的区域。
本文所述的任何Zika病毒疫苗或组合物可以以治疗有效量或治疗有效量的剂 量施用于受试者。如本文所用，疫苗的“治疗有效量”是导致受试者(例如本文 所述的那些)的期望应答或结果的任何量，包括但不限于预防感染、对Zika病毒 的免疫应答或增强的免疫应答，或预防或减少与Zika病相关的症状。
在一些实施方式中，本文所述的Zika病毒疫苗或组合物的治疗有效量是足以 产生抗原特异性抗体(例如抗Zika病毒抗体)的量。在一些实施方式中，治疗有 效量足以在受试者中提供血清保护；即产生足够的抗原特异性抗体以预防/防止感 染。在一些实施方式中，在接种疫苗的受试者中至少75％、80％、90％、95％、96％、 97％、98％或至少99％赋予血清保护。在一些实施方式中，血清保护定义为在斑块 减少中和试验(PRNT)中通过1：10以上稀释来自接种疫苗的受试者的血清而使 Zika病毒斑块的量降低50％以上。在一些实施方式中，有效量的Zika疫苗足以以 至少70％的可能性将受试者血清转化。在一些实施方式中，治疗有效量足以以至 少75％、80％、85％、90％、95％、96％、97％、98％或至少99％的可能性将受试 者血清转化。可以通过本领域已知的任何方法评估受试者是否已经血清转化，例 如从受试者获得血清样品并进行测定以检测抗Zika病毒抗体。在一些实施方式中， 如果来自受试者的血清样品含有超过阈值或预定基线的量的抗Zika病毒抗体，则 受试者被血清转化。如果受试者血清样品中的抗Zika病毒抗体(即抗Zika E蛋白 IgG抗体)与先前取自相同受试者的血清样品相比至少增加4倍，则受试者通常被 认为是血清转化的。
在一些实施方式中，通过进行斑块减少中和测试(PRNT)来评估受试者的血 清转化。简而言之，与对照血清/抗体相比，PRNT用于确定将Zika病毒斑块的量 减少50％所需的血清滴度(PRNT50)。PRNT50可以使用单层的Vero细胞或可以 感染Zika病毒的任何其它细胞类型/品系来进行。将受试者的血清稀释并与活的非 灭活的Zika病毒一起温育。血清/病毒混合物可以应用于Vero细胞并温育一段时 间。对单层Vero细胞上形成的斑块进行计数，并与不存在血清或对照抗体时Zika 病毒形成的斑块数量进行比较。PRNT50中1：10稀释血清的中和抗体的阈值通常 被认为是保护的证据(Hombach等，Vaccine(2005)23：5205-5211)。
在一些实施方式中，可以将Zika病毒配制成以组合物例如药物组合物施用。 如本文所用，术语“药物组合物”是指由至少一种活性成分(例如灭活Zika病毒) 与一种或多种非活性成分(其可包括一种或多种药学上可接受的赋形剂)的混合 或组合而得到的产品。
本发明的药物组合物包括疫苗，可以根据本领域熟知和常规实施的方法制备 (参见例如Remington：The Science and Practice of Pharmacy，Mack Publishing Co. 20nded.2000；和Ingredients of Vaccines-来自疾病控制和预防中心的情况说明书， 例如帮助疫苗改进其作用的佐剂和增强剂、帮助疫苗保持不变的防腐剂和稳定剂 (例如白蛋白、酚类、甘氨酸))。药物组合物优选在GMP条件下生产。通常在本 发明的药物组合物中使用治疗有效剂量的灭活Zika病毒制剂。通过本领域技术人 员已知的常规方法将灭活Zika病毒配制成药学上可接受的剂型。调整剂量方案以 提供最佳的期望应答(例如预防应答)。
本发明药物组合物中活性成分的剂量可以变化，以获得对特定受试者、组合 物和施用模式有效实现期望药物应答的活性成分的量，而对受试者没有毒性。所 选剂量水平取决于多种药代动力学因素，包括所用本发明特定组合物的活性、施 用途径、施用时间、所用具体化合物的排泄速率、治疗持续时间、与所用特定组 合物组合使用的其它药物、化合物和/或材料、所治疗的受试者的年龄、性别、体 重、状况、一般健康状况和既往病史等因素。
医师、兽医或其它受过训练的医生可以以低于实现期望治疗效果所需水平的 药物组合物中使用的灭活Zika病毒疫苗的剂量开始，并逐渐增加剂量，直到达到 期望效果(例如，产生抗-Zika病毒抗体)。通常，本文所述用于预防性治疗人群的 本发明组合物的有效剂量取决于许多不同因素，包括施用方式、靶位点、患者的 生理状态、患者是否是人或动物、所施用的其它药物以及所需的抗Zika病毒抗体 的效价。需要滴定剂量以优化安全性和有效性。在一些实施方式中，给药方案需 要皮下或肌肉内施用两次剂量的灭活Zika病毒，一次在第0天和一次在约第7天。 在一些实施方式中，给药方案需要在皮下施用两次剂量的灭活Zika病毒，一次在 第0天和一次在第14天。在一些实施方式中，给药方案需要在皮下施用两次剂量 的灭活Zika病毒，一次在第0天和一次在第28天。在一些实施方式中，向受试者施用一次灭活Zika病毒。
本文所述的任何Zika病毒疫苗或组合物可以在施用一种或多种佐剂的同时、 之前或之后施用于受试者。佐剂是增强受试者中对抗原或其它分子的应答(例如 免疫应答)的分子。在一些实施方式中，佐剂可稳定抗原或其它分子。确定Zika 病毒疫苗或其组合物是否与佐剂一起施用取决于各种因素(例如期望响应的类型 和程度)，并且对于本领域技术人员而言是显而易见的。在一些实施方式中，在施 用佐剂的同时、之前或之后施用本文所述的任何Zika病毒疫苗或组合物可以增强 病毒中和(抗Zika病毒)抗体的产生。在一些实施方式中，在施用佐剂的同时、 之前或之后施用本文所述的任何Zika病毒疫苗或组合物的受试者可能仅需要单次 施用待血清转化的Zika病毒疫苗或组合物(产生抗Zika病毒抗体的水平)。佐剂 的实例可以包括但不限于铝盐(氢氧化铝或磷酸铝)、磷酸氢钙、石蜡油、杀死的 细菌、细菌毒素、类毒素、细菌亚基、角鲨烯、硫柳汞、去垢剂、IL-1、IL-2、IL-12、 双组分佐剂(例如含有抗菌肽和TLR9激动剂(例如
Figure BDA0003695595590000141
)的双组分佐剂)、以 及诸如弗氏完全佐剂和弗氏不完全佐剂的组合。在一些实施方式中，Zika病毒疫 苗或组合物与氢氧化铝一起施用。在一些实施方式中，灭活Zika病毒疫苗或组合 物与磷酸铝盐一起施用。优选的铝盐是Cu含量降低的氢氧化铝，例如基于Zika 组合物的重量低于1.25ppb，是在WO2013/083726或Schlegl等，Vaccine 33(2015) 5989-5996中详细描述的佐剂。
在一些实施方式中，佐剂由两种组分组成。在一些实施方式中，所述双组分 佐剂包含抗菌肽和TLR9激动剂。在一些实施方式中，抗菌肽由氨基酸序列 KLKL5KLK(SEQ IDNO：71)提供。在一些实施方式中，TLR9激动剂是含脱氧 肌苷的免疫刺激性寡脱氧核酸分子(I-ODN)。在一些实施方式中，I-ODN包含核 酸序列(dIdC)13(SEQ ID NO：70)。在一些实施方式中，佐剂是
Figure BDA0003695595590000143
在一些实 施方式中，佐剂呈纳米颗粒形式(参见例如美国专利号8,765,148B2，其通过引用 整体并入)。在一些实施方式中，佐剂是与铝盐例如氢氧化铝组合的
Figure BDA0003695595590000142
即 KLKL5KLK(SEQ ID NO：71)和核酸序列(dIdC)13(SEQ ID NO：70)。
本文所述的Zika病毒疫苗或组合物可以与一种或多种疫苗同时施用于另一种 传染性病原体，例如与Zika病毒存在或被认为存在于相同地理区域中的另一种传 染性病原体。在一些实施方式中，另一种传染性病原体是受试者也处于接触风险 中的传染性病原体。在一些实施方式中，另一种传染性病原体由与Zika病毒相同 的节肢动物载体传播。在一些实施方式中，另一种传染性病原体是日本脑炎病毒、 黄热病病毒、登革病毒和/或基孔肯雅病毒。
同样在本公开内容的范围内的是用于预防性施用于受试者以例如预防或降低Zika病毒感染严重性的试剂盒。这样的试剂盒可以包括一个或多个包含含有灭活 Zika病毒的组合物的容器，例如灭活Zika病毒疫苗。在一些实施方式中，试剂盒 可以进一步包含一种或多种另外的含有第二组合物的附加物，例如第二疫苗。在 一些实施方式中，第二疫苗是另一种虫媒病毒的疫苗。在一些实施方式中，第二 疫苗是登革热病毒疫苗和/或基孔肯雅病毒疫苗。
在一些实施方式中，试剂盒可以包含根据本文所述的任何方法使用的说明书。 所包含的说明书可以包含对施用含有灭活Zika病毒的组合物的描述，以预防Zika 病毒感染、延缓其发作或降低其严重性。试剂盒可进一步包括基于鉴定该受试者 是否有风险暴露于Zika病毒或接触Zika病毒感染而选择适于施用的受试者的描 述。在其它实施方式中，说明书包括向有风险暴露于Zika病毒或接触Zika病毒的 受试者施用含有灭活Zika病毒的组合物的描述。
涉及使用含有灭活的Zika病毒的组合物的说明书通常包括关于预期治疗的剂量、施用方案和施用途径的信息。容器可以是单位剂量、散装包装(例如多剂量 包装)或亚单位剂量。在本发明的试剂盒中提供的说明书通常是标签或包装插入 物(例如包括在试剂盒中的纸张)上的书面说明书，但机器可读说明书也是可接 受的。
本公开的试剂盒处于合适的包装中。合适的包装包括但不限于小瓶、瓶子、 罐子和软包装等。还考虑到与特定装置(例如注射器或输注装置)组合使用的包 装。该容器可以具有无菌入口，例如该容器可以是具有可被皮下注射针头刺穿的 塞子的小瓶。如本文所述，组合物中的至少一种活性剂是灭活Zika病毒。
本发明的应用不限于以下描述中阐述的或附图中所示的部件的构造和布置的 细节。本发明能够具有其它实施方式并且能够以各种方式实践或执行。而且，这 里使用的措辞和术语是为了描述的目的，而不应该被认为是限制性的。本文中“包 括”、“包含”或“具有”、“含有”、“涉及”及其变体的使用意在涵盖其后列出的 项目及其等同物以及附加项目。
除非在此另外定义，否则与本公开相关使用的科学和技术术语应具有本领域 普通技术人员通常理解的含义。此外，除非上下文另有要求，单数形式的术语应 包括复数形式，复数形式的术语应包括单数形式。本公开的方法和技术通常根据 本领域众所周知的常规方法进行。通常，与本文所述的生物化学、酶学、分子和 细胞生物学、微生物学、病毒学、细胞或组织培养、遗传学和蛋白质以及核酸化 学有关的命名法和技术是本领域中众所周知和常用的。除非另有说明，否则本公 开的方法和技术通常根据本领域众所周知的常规方法进行，并如在本说明书全文 中引用和讨论的各种通常和更具体的参考文献中所述。
通过以下实施例进一步说明本发明，这些实施例决不应被解释为进一步的限 制。本申请通篇引用的所有参考文献(包括参考文献、授权专利、公开的专利申 请和共同未决的专利申请)的全部内容明确地通过引用并入本文，特别是对于上 文提到的教导。然而，任何参考文献的引用不打算承认该参考文献是现有技术。
表1.处理缓冲液和储液的概述。
Figure BDA0003695595590000161
表2.缩写。
Figure BDA0003695595590000162
Figure BDA0003695595590000171
*白利糖度(°Bx)是水溶液的糖含量。一白利糖度是100克溶液中的1克蔗 糖，代表溶液的质量百分比强度。°Bx对应于百分比(w/w)的蔗糖含量，例如45°Bx 等于45％(w/w)蔗糖。
表A.Zika病毒测序的引物：小写字母表示碱基不包含在ZIKA中，但在需要 时含有用于以后克隆的限制性位点(因此提供两个Tms)。
Figure BDA0003695595590000181
Figure BDA0003695595590000191
Figure BDA0003695595590000201
序列
SEQ ID NO：1
鱼精蛋白的典型形式
PRRRRSSSRP VRRRRRPRVS RRRRRRGGRR RR
下文提供的是可用于本文所述的方法、组合物和/或疫苗中的Zika病毒基因组 的核酸序列的实例。
SEQ ID NO：2
KU321639.1Zika病毒株ZikaSPH2015，巴西，完整的基因组
GTTGTTACTGTTGCTGACTCAGACTGCGACAGTTCGAGTTTGAAGCGAA AGCTAGCAACAGTATCAACAGGTTTTATTTGGATTTGGAAACGAGAGTTTCTG GTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGC TAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGC CAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCAATTCTA GCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATG GGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAAGAA AGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAAGAG ACGGGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCT ATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGACA GAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAA GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTA TGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGG TGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAA GGTGAAGCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTA GGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAA AGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTA GCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAGTC ATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATA GGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTG ATATTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAACC GACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGT AAGATCCTACTGCTATGAGGCATCAATATCAGACATGGCTTCGGACAGCCGCT GCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGT CTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTT TGGCAAAGGGAGTCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATG ACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAG TTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAAC TGATGAGAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCCGA AGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGAC AGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGT TGGTTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGGGC AGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAA GGACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAAGG AGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGC AAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAA CTTAGATTGAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCACATTCAC CAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTA CGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACAT GCAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCACT GAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGG ACTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCA CAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGC CAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGG AGGCGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGCT TTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAAC GTTGCTGATGTGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGT GCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCTGCTGAT GTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGG GTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCATC CTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATG GTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCA GTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACG GTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGC CCGTGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGC ACTTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACAC ACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAG GATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAG ATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAG GAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACA CATGGAGGCTGAAGAGGGCCCATCTGATCGAGATGAAAACATGTGAATGGCC AAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATA CCCAAGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACA GGACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTG AGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAG GACCATCTCTGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGT GCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCTG TTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGT AAGGTCAATGGTGACTGCAGGATCAACTGATCACATGGATCACTTCTCCCTT GGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATG ACCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCT GGGAGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCC ACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAG CGGCATTCAAAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAAT TGGACACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAA CTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCT TTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGATAACATCAC CTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTG GCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGA AGGGAAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGAC TAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGGCTGCTGTTGCT CACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGT TGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATG GCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAG GAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAA AAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATG AGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGAT CATACTCAAGGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATAC CCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCA CAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGT TGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACA AAAGGATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGA GATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCG CCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGA GAGCGAGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGG ACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAAT CCTAGACAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATC AAAAATGGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAG ACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTG TCTTAGACTTGCATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAAT AGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTT ATATGACAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTT AATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCA ACTATAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAG CAGCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCA TCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAA CTCACCAATTATGGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTC AGGCTTTGATTGGGTGACGGATTATTCTGGAAAAACAGTTTGGTTTGTTCCAA GCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAAC GGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACAA AACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCGC CAACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTC ATACTTGATGGCGAGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATGC CAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGG AGATGAGTATCTGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCA CACTGGCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGCCT CATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGA GAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGAAA AGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATAAC CTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATG GAAGACAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGAGT GCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTG AAGTCATTCAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGGAA GCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTT ACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTT GGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGAGGAAC AAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCAT GGCTCATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCCTCATT GTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGAT CTCCCCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTG GGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAGTGAC CTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATGGGATTCTCA ATGGACATTGACCTGCGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGAC AACTTTCATTACCCCAGCCGTCCAACATGCAGTGACCACTTCATACAACAACT ACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAA AGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTT GCTACTCACAATTAACGCCCCTGACCCTAATAGTGGCCATCATTTTGCTCGTG GCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTG CCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGATGGAAT AGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAA GATGGGACAGGTGCTACTCATGGCAGTAGCCGTCTCCAGCGCCATACTGTCG CGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCCGCAACT TCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCA CTTCACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCTAATC TACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACA GGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCC CTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGAA GAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTG TCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAG CCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTACT ACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAG GCCCTGGTCATGAAGAACCCGTGTTGGTGCAAAGCTATGGGTGGAACATAGT CCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGTGAC ACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAG CACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGAC CAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAA ACCCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCA CTCTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCA ACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGA CGGGCCTAGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGC ACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTA ACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACG AGAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCAC ACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAA CCCTGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACAC CGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAG ACCCCCAAGAAGGTACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTG GAAAGAGCTAGGCAAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTT CATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAA AAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCT CTAGTGGACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAA GGCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAG AGTTCGAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGA ACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCT AGAAGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCT GGCTGGGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCA CCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTA CACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAA AACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGACAAGT TGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGA ATATGGAGGCTGAGGAAGTCCTAGAGATGCAAGACTTGTGGCTGCTGCGGAG GTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAA ACGAATGGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACA CACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGT TTTGCTCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCATTGT GGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCA GGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGC AAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGGCCAA TGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACCT GGTCAATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGT GTGGAACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAGACCCC AGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGG TGTGGATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGAGAACATTA AAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACAT GGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCT GGAGTGCTGTAAGCACCAATCTTAATGTTGTCAGGCCTGCTAGTCAGCCACA GCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAAAC CAAGCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGC CTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGG CGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGC CTGAACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAG GAGA
SEQ ID NO：3
KU497555.1Zika病毒分离株巴西-ZKV2015，巴西，完整的基因组
CCAATCTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAG CAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCAT GAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAA CGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCC GGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTT TTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTT CAGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATC TGGCTGCCATGCTGAGAATAATCAATGCCAGGAAGGAGAAGAAGAGACGAG GCGCAGATACTAGTGTCGGAATCGTTGGCCTCCTGCTGACCACAGCTATGGC AGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGACAGAAAC GATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGTTA TATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAAT GCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAA CACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGA AGCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAG CTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAAAGCAC TTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGC AGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAGTCATATAC TTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT CAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTT GTCTTGGAACATGGGGGTTGTGTCACCGTAATGGCACAGGACAAACCGACTG TCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATC CTACTGCTATGAGGCATCAATATCAGACATGGCTTCGGACAGCCGCTGCCCAA CACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGTCTGCAA AAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAA AGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGG AAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAGTTCATG GCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGA GAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCCGAAGCCAC CCTGGGGGGTTTTGGAAGCTTAGGACTTGATTGTGAACCGAGGACAGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCA CAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGGGCAGACACC GGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCA CATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGACTCAAGAAGGAGCAGTT CACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGCAAAGGGA AGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATT GAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCACATTCACCAAGATC CCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGCAGGG ACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTC TGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCACTGAAAGCAC TGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACA TTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTG GCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCT CAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGCTTTCAAATCAT TGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTGCTGATG TGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTT AGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCTGCTGATGTGGGGTGCT CGGTGGACTTCTCAAAGAAGGAGACGAGATGTGGTACAGGGGTGTTCGTCTA TAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCTCCC CGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATGGTATCTGCGGGA TCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGA GCTTAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCCCGTGCCTGTGA ACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAG CAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACACACTGAAGGAAT GCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGTT CGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAG AGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAGGAGGCTGTACA CAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTG AAGAGGGCCCATCTGATCGAGATGAAAACATGTGAATGGCCAAAGTCCCAC ACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATACCCAAGTCTTT AGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAAT GAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTGAGGAATGCCC AGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCATCTCT GAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCTGTTGGTATGGA ATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATG GTGACTGCAGGATCAACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGT GATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATGACCACAAAGAT CATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTT CAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCCACCTTCGCGGA AATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGCATTCAAA GTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCG TGAAAGCATGCTGCTGGCCTTGGCCTCGTGTTTTTTGCAAACTGCGATCTCCG CCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGACAACATCACCTTGGCAATCC TGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTGGCGTGGAGAG CAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGG CAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGACTAACCGCTGTG AGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCACAAGGAGT GGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATAT GCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATGGCTGGGCCCAT GGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTG GACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAA GTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATGAGAGTGGTGACT TCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGT GGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATACCCTTTGCAGCTG GAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGA TGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTA CAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGTTGGAGTGGGAGTT ATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCGCGC TGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGG ATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCGCCTGGGACGGGCA CAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACAT CCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTT GCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAATCCTAGACAAGTGTG GGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATAAAAAATGGGAGTTA TGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAGACTCCTGTTGAGTG CTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGACTTGCAT CCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCC ATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTG CTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGC AGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCC ACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCAACTATAATCTGTAT ATTATGGATGAGGCCCACTTCACAGATCCCTCAAGCATAGCAGCAAGAGGAT ACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCATCTTCATGACCGC CACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCACCAATTATG GACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGG GTGACGGATCATTCTGGAAAAACAGTTTGGTTTGTTCCAAGCGTGAGGAACG GCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCT CAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCGCCAACTTTAAAGCT GACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCG AGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATGCCAGCGCTGCCCA GAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGATGAGTACCT GTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGA AGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGCCTCATAGCCTCGC TCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCT TAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCT TCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATAACCTACACAGATA GAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGTGT GCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGAGTGCTCAAACCGA GGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGAAGTCATTCAA GGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGGAAGCCATTGACAA CCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGC GGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTG GGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGAGGAACAAGGGCATAG GGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTG GCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCC TATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGATCTCCCCAGGA CAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTA CCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAGTGACCTAAGCCATCT AATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACATTGA CCTGCGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTA CCCCAGCCGTCCAACATGCAGTGACCACTTCATACAACAACTACTCCTTAATG GCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCAT TCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAA TTAACACCCCTGACCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTACAT GTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAG AACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGATGGAATAGTGGTGACT GACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATGGGACAG GTGCTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCT GGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCCGCAACTTCCACTTTGT GGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTG TAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAA CAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACAGGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTC TACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGAAGAGGCCCGC CGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCGAGGA AGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGA AAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTACTACGCCGCCA CCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTC ATGAAGAACCCGTGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAA GAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGTGACACGTTGCTG TGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGC TCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGACCAGGAGCCTT TTGCATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAAACCCTGGAG CGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGC AACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCAACACCATAA AAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTA GGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGG CTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTAACCGCAT TGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAAAACCA CCCATATAGGACATGGGCTTACCATGGAAGCTATGTGGCCCCCACACAAGGG TCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAACCCTGGG ATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATGG TCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAGACCCCCA AGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAG CTAGGCAAACACAAACGACCACGAGTCTGTACCAAAGAAGAGTTCATCAAC AAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAAAAAGAGT GGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGG ACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTAC AACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAAGGCCAA GGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCG AAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAGA GATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGG GACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCACCAACC AAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACATA CCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAAACAGT TATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGACAAGTTGTCACT TACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGA GGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTGCGGAGGTCAGA GAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACGAAT GGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCA CATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACACACAAG AGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCT CCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCAGGGGCG GGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGT GGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGGCCAATGCCATT TGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACCTGGTCAA TCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGA ACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAGACCCCAGTTA CGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGG ATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAAT ACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGACT ACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGT GCTGTGAGCACCAATCTTAATGTTGTCAGGCCTGCTAGTCAGCCACAGCTTG GGGAAAGCTGTGCAGCCTGTGACCCCTCCAGGAGAAGCTGGGTAACCAAGC CTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGA GCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGGCGCAGG ATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACT GGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGACCC CCCGGAAAACGCAAAACAGCATATTGACGCTGGGAAAGACCAGAGACTCCA TGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAATAGCGGCGGCC GGTGTGGGGAAATCCA
SEQ ID NO：4
KU501215.1 Zika病毒株PRVABC59，波多黎各，完整的基因组
GTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGAA AGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCT GGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATG CTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTG CCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCT AGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGAT GGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAACAATAAAGAAGTTCAAGA AAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAAGA GACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGC TATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGACA GAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAA GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTA TGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGG TGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAA GGTGAAGCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACCA GGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAA AGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTA GCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAGTC ATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATA GGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTG ATGTTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAACC GACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGT AAGATCCTACTGCTATGAGGCATCAATATCAGACATGGCTTCTGACAGCCGCT GCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGT CTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTT TGGCAAAGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATG ACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAG TTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAAC TGATGAGAATAGAGCGAAAGTTGAGATAACGCCCAATTCACCGAGAGCCGA AGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGAC AGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGT TGGTTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGGGC AGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAA GGACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAAGG AGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGC AAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAA CTTAGATTGAAGGGCGTGTCATACTCCTTGTGTACTGCAGCGTTCACATTCAC CAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTA CGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACAT GCAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCACT GAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGG ACTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCA CAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGG AGGCGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGCT TTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAAC GTTGCTGATGTGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGT GCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCTGCTGAT GTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGG GTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCATC CTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATG GTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCA GTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACG GTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGC CCGTGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGT ATTTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACAC ACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAG GATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAG ATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAG GAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACA CATGGAGGCTGAAGAGGGCCCATCTGATCGAGATGAAAACATGTGAATGGCC AAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATA CCCAAGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACA GGACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTG AGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAG GACCATCTCTGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCTG TTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGT AAGGTCAATGGTGACTGCAGGATCAACTGATCACATGGACCACTTCTCCCTT GGAGTGCTTGTGATCCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATG ACCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCT GGGAGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCC ACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAG CGGCATTCAAAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAAT TGGACACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAA CTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCT TTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGATAACATCAC CTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTG GCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGA AGGGAAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGAC TAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCT CACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGT TGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATG GCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAG GAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAA AAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATG AGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGAT CATACTCAAGGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATAC CCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCA CAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGT TGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACA AAAGGATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGA GATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCG CCTGGGATGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGA GAGCGAGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGG ACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAAT CCTAGACAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATC AAAAACGGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAG ACTCCTGTTGAGTGCTTCGAGCCCTCGATGCTGAAGAAGAAGCAGCTAACTG TCTTAGACTTGCATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAAT AGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAGGCCCTTAGAGGGCTTCCAGTGCGTT ATATGACAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTT AATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCA ACTATAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAG CAGCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCA TCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAA CTCACCAATTATGGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTC AGGCTTTGATTGGGTGACGGATCATTCTGGAAAAACAGTTTGGTTTGTTCCA AGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAA CGGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACA AAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCG CCAACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGT CATACTTGATGGCGAGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATG CCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTG GAGATGAGTATCTGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGC ACACTGGCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGC CTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGG GAGAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGA AAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATA ACCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAA TGGAAGACAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGA GTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCC TGAAGTCATTCAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAG TGATGGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGG AAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGC CTTACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATAATGCT TTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTCTTCGTCTTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGC GCATGGCTCATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCC TCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA AGATCTCCCCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTC TTCTGGGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAG TGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATT CTCAATGGACATTGACCTGCGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCT TGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGACCACCTCATACAA CAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGCATGGGCAAAGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGAT AGGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCCATCATTTTGC TCGTGGCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCG TGCTGCCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGA TGGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAG AAAAAGATGGGACAGGTGCTACTCATAGCAGTAGCCGTCTCCAGCGCCATAC TGTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCTCTGATCACAGCCG CAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTAC AGCCACTTCACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTC TAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTG GAACAGGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGT CGGCCCTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAG AGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGC TGTGTCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCT GCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGT TACTACGTCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAA GGAGGCCCTGGTCATGAAGAACCCGTGTTGGTGCAAAGCTATGGGTGGAAC ATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGT GTGACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGA AGAAGCACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAA AAGACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGA TGGAAACCCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAG TGCCACTCTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAA AAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCG CATGGACGGGCCTAGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGC TCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCA TTGGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTT TGACGAGAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCC CCCACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGT CAAAACCCTGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACA CCACACCGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGG TGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTG GTTGTGGAAAGAGCTAGGCAAACACAAACGGCCACGAGTCTGCACCAAAGA AGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAA GAGGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTT CTGGGCTCTAGTGGACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCC AGAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAAT TTGGAAAGGCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAG ATTTCTAGAGTTCGAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGA TATGTCCTAGAAGAGATGAGTCGTATACCAGGAGGAAGGATGTATGCAGATG ACACTGCTGGCTGGGACACCCGCATTAGCAGGTTTGATCTGGAGAATGAAGC TCTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATA ATCAAGTACACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAA AAGGGAAAACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCG GACAAGTTGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTC ATTCGGAATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGC TGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATA GGCTCAAACGAATGGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGA TGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGA AGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAA GTTCCGTTTTGCTCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGT CTCTCCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCA TATGCGCAAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGAT GGCCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGA ACTACCTGGTCAATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGC TTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACA AGACCCCAGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAG ACTTGTGGTGTGGATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGA GAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAA AAGTACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGT CTACACCTGGAGTGCTGTAAGCACCAATCTTAATGTTGTCAGGCCTGCTAGTC AGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCT GGGAAACCAAGCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCC ATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGC TTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAAT CTGGGGCCTGAACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTG GTTAGAGGA
SEQ ID NO：5
KU509998.1Zika病毒株海地/1225/2014，海地，完整的基因组
GTTGTTACTGTTGCTGACTCAGACTGCGACAGTTCGAGTTTGAAGCGAA AGCTAGCAACAGTATCAACAGGTTTTATTTGGATTTGGAAACGAGAGTTTCTG GTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGC TAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGC CAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCAATTCTA GCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATG GGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAAGAA AGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAAGAG ACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCT ATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGACA GAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAA GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTA TGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGG TGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAA GGTGAAGCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTA GGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAA AGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTA GCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAGTC ATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATA GGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTG ATGTTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAACC GACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGT AAGATCCTACTGCTATGAGGCATCAATATCAGACATGGCTTCGGACAGCCGCT GCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGT CTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTT TGGCAAAGGGAGTCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATG ACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAG TTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAAC TGATGAGAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCCGA AGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGAC AGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGT TGGTTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGGGC AGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAA GGACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAAGG AGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGC AAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAA CTTAGATTGAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCACATTCAC CAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTA CGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACAT GCAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCACT GAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGG ACTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCA CAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGC CAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGG AGGCGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGCT TTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAAC GTTGCTGATGTGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGT GCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCTGCTGAT GTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGG GTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCATC CTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATG GTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCA GTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACG GTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGC CCGTGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGC ACTTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACAC ACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAG GATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAG ATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAG GAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACA CATGGAGGCTGAAGAGGGCCCATCTGATCGAGATGAAAACATGTGAATGGCC AAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATA CCCAAGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACA GGACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTG AGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAG GACCATCTCTGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGT GCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCTG TTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGT AAGGTCAATGGTGACTGCAGGATCAACTGATCACATGGATCACTTCTCCCTT GGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATG ACCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCT GGGAGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCC ACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAG CGGCATTCAAAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAAT TGGACACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAA CTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCT TTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGATAACATCAC CTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTG GCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGA AGGGAAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGAC TAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGGCTGCTGTTGCT CACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGT TGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATG GCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAG GAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAA AAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATG AGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGAT CATACTCAAGGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATAC CCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCA CAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGT TGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACA AAAGGATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGA GATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCG CCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGA GAGCGAGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGG ACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAAT CCTAGACAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATC AAAAATGGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAG ACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTG TCTTAGACTTGCATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAAT AGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTT ATATGACAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTT AATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCA ACTATAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAG CAGCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCA TCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAA CTCACCAATTATGGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTC AGGCTTTGATTGGGTGACGGATTATTCTGGAAAAACAGTTTGGTTTGTTCCAA GCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAAC GGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACAA AACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCGC CAACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTC ATACTTGATGGCGAGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATGC CAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGG AGATGAGTATCTGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCA CACTGGCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGCCT CATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGA GAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGAAA AGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATAAC CTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATG GAAGACAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGAGT GCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTG AAGTCATTCAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGGAA GCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTT ACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTT GGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGAGGAAC AAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCAT GGCTCATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCCTCATT GTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGAT CTCCCCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTG GGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAGTGAC CTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATGGGATTCTCA ATGGACATTGACCTGCGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGAC AACTTTCATTACCCCAGCCGTCCAACATGCAGTGACCACTTCATACAACAACT ACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAA AGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTT GCTACTCACAATTAACGCCCCTGACCCTAATAGTGGCCATCATTTTGCTCGTG GCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTG CCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGATGGAAT AGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAA GATGGGACAGGTGCTACTCATGGCAGTAGCCGTCTCCAGCGCCATACTGTCG CGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCCGCAACT TCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCA CTTCACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCTAATC TACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACA GGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCC CTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGAA GAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTG TCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAG CCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTACT ACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAG GCCCTGGTCATGAAGAACCCGTGTTGGTGCAAAGCTATGGGTGGAACATAGT CCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGTGAC ACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAG CACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGAC CAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAA ACCCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCA CTCTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCA ACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGA CGGGCCTAGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGC ACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTA ACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACG AGAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCAC ACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAA CCCTGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACAC CGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAG ACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTG GAAAGAGCTAGGCAAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTT CATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAA AAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCT CTAGTGGACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAA GGCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAG AGTTCGAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGA ACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCT AGAAGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCT GGCTGGGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCA CCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTA CACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAA GACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGACAAGT TGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGA ATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTGCGGAG GTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAA ACGAATGGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACA CACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGT TTTGCTCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCATTGT GGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCA GGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGC AAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGGCCAA TGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACCT GGTCAATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGT GTGGAACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAGACCCC AGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGG TGTGGATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGAGAACATTA AAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACAT GGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCT GGAGTGCTGTAAGCACCAATCTTAATGTTGTCAGGCCTGCTAGTCAGCCACA GCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAAAC CAAGCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGC CTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGG CGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGC CTGAACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAG GAGA
SEQ ID NO：6
KU527068.1 Zika病毒株Natal RGN，巴西：Rio Grande do Norte，Natal，完整 的基因组
AGTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGA AAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTC TGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATAT GCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCT GCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCAATT CTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAG ATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAA GAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAA GAGACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACA GCTATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGA CAGAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAAT AAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAG CTATGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTT GGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAA AAGGTGAAGCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCAC TAGGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACAC AAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCG TTAGCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAG TCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCA TAGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGT TGATGTTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAA CCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAG GTAAGATCCTACTGCTATGAGGCATCAATATCAGACATGGCTTCGGACAGCCG CTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATAT GTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTT TTTGGCAAAGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAA TGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTC AGTTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAA ACTGATGAGAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCC GAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGG ACAGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTG GTTGGTCCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGG GCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTC AAGGACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAA GGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGT GCAAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATA AACTTAGATTGAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCACATTC ACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAG TACGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGAC ATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCA CTGAAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGG GGACTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGG CACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGT GCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTT GGAGGCGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAG CTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATCCTCATTGGA ACGTTGCTGATGTGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTAT GTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCTGCTG ATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAG GGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCA TCCTGACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGAT GGTATCTGCGGGATCTCCTCTGTTTCAAGAATGGAGAACATCATGTGGAGATC AGTAGAAGGGGAGCTCAACGCAATCTTGGAAGAGAATGGAGTTCAACTGAC GGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTG CCCGTGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCG TACTTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACA CACTGAAGGAATGCCCACTCGAACATAGAGCATGGAACAGCTTTCTTGTGGA GGATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAA GATTATTCATTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGGAA GGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGAC ACATGGAGGCTGAAGAGGGCCCATCTAATCGAGATGAAAACATGTGAATGGC CAAAGTCCCACACATTGTGGGCAGATGGAATAGAAGAGAGTGATCTGATCAT TCCCAAGTCTTTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTAC AGGACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTT GAGGAATGCCCGGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGA GGACCATCTCTGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCT GTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAG TAAGGTCAGTGGTGACTGCAGGATCAACTGATCACATGGATCACTTCTCCCTT GGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATG ACCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCT GGGAGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGCGCC ACCTTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAG CGGCATTCAAAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAAT TGGACACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAA CTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGATAACATCAC CTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTG GCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGA AGGGAAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGAC TAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCT CACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGT TGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATG GCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAG GAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAA AAGATGCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATG AGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGAT CATACTCAAGGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATAC CCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCA CAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGT TGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACA AAAGGATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGA GATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCG CCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGA GAGCGAGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGG ACATTGGAGCGGTTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAAT CCTAGACAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATC AAAAATGGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAG ACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTG TCTTAGACTTGCATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAAT AGTCCGTGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTT ATATGACAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTT AATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCA ACTATAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAG CAGCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCA TCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAA CTCACCAATTATGGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTC AGGCTTTGATTGGGTGACGGATCATTCTGGAAAAACAGTTTGGTTTGTTCCA AGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAA CGGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACA AAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCG CCAACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGT CATACTTGATGGCGAGAGAGTCATTTTGGCTGGACCCATGCCTGTCACACATG CCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTG GAGATGAGTATCTGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGC ACACTGGCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGC CTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGG GAGAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGA AAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATA ACCTACACAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAA TGGAAGACAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGA GTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCC TGAAGTCATTCAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAG TGATGGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGG AAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGC CTTACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCT TTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGAGG AACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGC GCATGGCTCATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCC TCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAA AGATCTCCCCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTC TTCTGGGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAG TGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGAGCAACCATAGGATT CTCAATGGACATTGACCTGCGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCT TGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGACCACTTCATACAAC AACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGG GCAAAGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATA GGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCCATCATTTTGCT CGTGGCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGT GCTGCCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGAT GGAATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGA AAAAGATGGGACAGGTGCTACTCATAGCAGTAGCAGTCTCCAGCGCCATACT GTCGCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCCGC AACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACA GCCACTTCACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCT AATCTACATAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACAGGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTC GGCCCTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGA GAAGAGGCCCGCCGCGCCCTCAAGGATGGTGTGGCAACGGGAGGCCATGCT GTGTCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTG CAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTT ACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAG GAGGCCCTGGTCATGAAGAACCCGTGTTGGTGCAAAGCTATGGGTGGAACAT AGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGT GACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAG AAGCACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAA GACCAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATG GAAACCCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTG CCACTCTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCAT GGACGGGCCTAGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCT GGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTG GTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGA CGAGAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCC ACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAA AACCCTGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCA CACCGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGC CAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTT GTGGAAAGAGCTAGGCAAACACAAACGGCCACGAGTCTGTACCAAAGAAG AGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGA GGAAAAAGAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTG GGCTCTAGTGGACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGA GTTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTG GAAAGGCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATT TCTAGAGTTCGAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGA GAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATAT GTCCTAGAAGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACA CTGCTGGCTGGGACACCCGCATCAGCAGGTTCGATCTGGAGAATGAAGCTCT AATCACCAACCAAATGGAGAAAGGGCATAGGGCCTTGGCATTGGCCATAATC AAGTACACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAA GGGAAAACAGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGA CAAGTTGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCAT TCGGAATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTG CGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGG CTCAAACGAATGGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATG ATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAG GACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTT CCGTTTTGCTCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCA TTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTC TCCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATAT GCGCAAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGG CCAATGCCATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACT ACCTGGTCAATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTG TGGTGTGGAACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAGA CCCCAGTTACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTT GTGGTGTGGATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGAGAAC ATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGT ACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTAC ACCTGGAGTGCTGTAAGCACCAATCTTAATGTTGTCAGGCCTGCTAGTCAGC CACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGG AAACCAAGCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATG CTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCATGCGCTTG GAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTG GGGCCTGAACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTT AGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGCTGGGAAAGAC CAGAGACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAA TAGCGGCGGCCGGTGTGGGGAAATCCATGGGTCTT
SEQ ID NO：7
KU681081.3 Zika病毒分离株Zika病毒/H.sapiens-tc/THA/2014/SV0127-14，泰国，完整的基因组
AGTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGA AAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTC TGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATAT GCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCT GCCAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATT CTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAG ATGGGGTTCAGTGGGAAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAA GAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAA GAGACGAGGCACAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACA GCTATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGA CAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACCACACTGGGGATGAAT AAGTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAG CTATGAATGCCCTATGCTGGATGAGGGGGTAGAACCAGATGACGTCGATTGTT GGTGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAA AAGGTGAAGCACGGAGATCCAGAAGAGCTGTGACGCTCCCCTCCCATTCCA CTAGGAAGCTGCAAACGCGGTCGCAGACCTGGTTGGAATCAAGAGAATACA CAAAGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGC GTTAGCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAA GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTG CATAGGAGTCAGTAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACA AACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGG AGGTAAGATCCTACTGCTATGAGGCATCAATATCGGACATGGCTTCGGACAGC CGCTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAAT ATGTCTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGAC TTTTTGGCAAAGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAA AATGACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTG TCAGTTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATG AAACTGATGAGAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAG CCGAAGCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGA GGACAGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAACAACAAGCAC TGGTTGGTTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACACTG GGGCAGACACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAA GAAGGAGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGAT GGTGCAAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATG GATAAACTTAGATTGAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCAC ATTCACCAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGT ACAGTACGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGT GGACATGCAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTA ATCACTGAAGGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCAT TTGGGGACTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCA CTGGCACAGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAG AGGTGCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATC AGTTGGAGGCGTTCTTAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGA GCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTGCTGATGTGGTTGGGTCTGAATACAAAGAATGGATCTATTTCCC TTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCC GCTGATGTGGGGTGCTCGGTGGACTTCTCAAAGAAGGAAACGAGATGCGGT ACAGGGGTGTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGT ACCATCCTGACTCCCCTCGTAGATTGGCAGCAGTAGTCAAGCAAGCCTGGGA AGATGGTATCTGTGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGA GATCAGTAGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAAC TGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGA GATTGCCCGTGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGA AATCGTACTTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGG TGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTT GTGGAGGATCATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTA GAGAAGATTATTCACTAGAGTGTGATCCAGCCGTCATTGGAACAGCTGTTAA GGGAAAGGAGGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAA GAACGACACATGGAGGCTGAGGAGGGCCCACCTGATCGAGATGAAAACATG TGAATGGCCAAAGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGAT CTGATCATACCCAAGTCTTTAGCTGGGCCACTCAGCCATCACAACACCAGAG AGGGCTACAGGACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAA TTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTG GAACAAGAGGACCATCTCTGAGATCAACCACTGCAAGCGGAAGGGTGATCG AGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCACTGTCGTTCCGGGCTAA AGATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAG TAACTTAGTAAGGTCAATGGTGACTGCAGGATCAACTGATCACATGGATCACT TTTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAA GAGAATGACCACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCT ATGATCCTGGGAGGATTTTCAATGAGTGATCTGGCTAAGCTTGCAATTTTGAT GGGTGCCACCTTTGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCG CTGGTAGCGGCATTCAAAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAG AGCTAATTGGACACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTT TTGCAAACTGCGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATG GTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGAC AATATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACAC TGCTTGTGGCGTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTCATGCTCCT CTCTCTGAAGGGGAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCC CTGGGACTAACCGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGC TGTTGCTCACAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCA CAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATA GAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGG TCTCAGGAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATG GGAAAAAGATGCGGAAGTTACTGGAAACAGTCCCCGGCTCGATGTGGCACT AGATGAGAGTGGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGA GAGATCATACTCAAAGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGC CATACCCTTTGCAGCTGGAGCGTGGTACGTATACGTGAAAACTGGAAAAAGG AGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAG ACCACAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACAC AAGTTGGAGTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCATGTCACAAAAGGATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTG GGGAGATGTCAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGAT GCCGCCTGGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGA GAGAGAGCGAGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGGAT GGGGACATTGGAGCGGTTGCGCTGGACTATCCAGCAGGAACTTCAGGATCTC CAATCCTAGACAAGTGTGGGAGAGTGATAGGACTCTATGGCAATGGGGTCGT GATCAAGAATGGGAGTTATGTCAGTGCCATCACCCAAGGGAGGAGGGAGGA AGAGACTCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTA ACTGTCTTAGACTTGCATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTG AAATAGTCCGTGAAGCCATAAAAACGAGACTCCGTACTGTGATCTTAGCTCC AACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGT GCGTTATATGACAACAGCAGTCAATGTCACCCATTCTGGGACAGAAATCGTT GACTTAATGTGCCATGCCACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCAACTATAATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAG TATAGCAGCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCAGCT GCCATCTTCATGACCGCCACGCCACCAGGAACCCGTGACGCATTCCCGGACT CCAACTCACCAATTATGGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGA GCTCAGGCTTTGATTGGGTGACGGATCATTCTGGAAAAACAGTTTGGTTTGT CCCAAGCGTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGG AAAACGGGTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAA AACAAAACATCAAGAGTGGGACTTCGTCGTGACAACTGACATTTCAGAGAT GGGCGCCAACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAG CCGGTCATACTTGATGGCGAGAGAGTCATTCTGGCTGGACCCATGCCTGTCA CACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACA AACCTGGAGATGAGTATCTGTATGGAGGTGGGTGCGCAGAGACTGATGAAGA CCATGCACACTGGCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAG ATGGCCTCATAGCCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATT GAGGGAGAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTC ATGAAAAGAGGAGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCG GAATAACCTACACAGATAGAAGATGGTGCTTTGATGGCATGACCAACAACAC CATAATGGAAGACAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAA AAGAGTGCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGC GGCCCTGAAGTCATTCAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTT TGGAGTGATGGAAGCCCTGGGAACACTGCCAGGACACATGACGGAGAGATT CCAGGAAGCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAG CAGGCCTTACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATT ATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGAT GCGGAACAAGGGCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGC CAGCGCATGGCTCATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGC GTCCTCATTGTTGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAA GCAAAGATCCCCCCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTA GGTCTTCTGGGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAA AGAGTGACCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATAG GATTCTCAATGGACATTGACCTGCGGCCAGCCTCGGCCTGGGCCATCTATGCT GCCCTGACAACTTTCATTACCCCAGCCGTCCAACATGCAGTGACCACTTCATA CAACAACTACTCCTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGT ATGGGCAAAGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAA TGATAGGTTGCTACTCACAATTAACACCCCTGACCCTAATAGTGGCTATCATTT TGCTCGTGGCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGC GCGTGCTGCCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGT GGATGGAATAGTGGTGACTGACATTGACACAATGACTATTGACCCCCAAGTG GAGAAAAAGATGGGACAGGTGCTACTCATAGCAGTAGCCGTCTCCAGCGCCA TACTGTCGCGGACCGCCTGGGGGTGGGGGGAAGCTGGGGCCCTGATCACAG CTGCAACTTCCACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTC TACAGCCACTTCACTGTGCAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTT CTCTAATCTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGG GTGGAACAGGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAG ATGTCGGCCCTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGT GCAGAGAAGAGGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGC CATGCTGTGTCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGA TACCTGCAGCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCT GGAGTTACTACGCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACAC AAAAGGAGGCCCTGGTCATGAAGAACCCATGTTGGTGCAAAGCTATGGGTG GAACATAGTCCGTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAG CCGTGTGACACGTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGT GGAAGAAGCACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGA AAAAAGACCAGGAGCCTTTTGTGTAAAAGTGTTGTGCCCATACACCAGCACT ATGATGGAAACCCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTC AGAGTGCCACTCTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAG CGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGG GGCGCATGGACGGGCCCAGGAGGCCAGTGAAATATGAGGAGGATGTGAATC TCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAA GATCATTGGTAACCGCATTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGG TTCTTTGACGAGAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGA GGCCCCTACACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTC CTGTCAAAACCCTGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCG ACACCACACCGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACCA GGGTGCCAGACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTC CTGGTTGTGGAAAGAGCTAGGCAAACACAAACGGCCACGAGTCTGTACCAA AGAAGAGTTCATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTT GAAGAGGAAAAAGAGTGGAAGACCGCAGTGGAAGCTGTGAACGATCCAAG GTTCTGGGCTCTAGTGGACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTG CCAGAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGG AATTTGGAAAGGCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGC TAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTAAATGAGGATCACTGGATGG GGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCG GATATGTCCTAGAAGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGA TGACACTGCTGGCTGGGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAA GCTTTAATCACCAACCAAATGGAGAAAGGGCACAGGGCCTTAGCATTGGCCA TAATCAAGTACACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGA AAAAGGGAAGACAGTTATGGACATTATTTCAAGACAAGACCAAAGGGGGAG CGGACAAGTTGTCACTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAA CTCATTCGGAATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGC TGCTGCGGAGGTCAGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGG ATAGGCTCAAACGAATGGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAAT TGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTA GGAAGGACACACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAA GAAGTTCCGTTTTGTTCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGA GGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCG TGTCTCTCCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAG TCATATGCGCAAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACT GATGGCCAATGCCATCTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGG AGAACTACCTGGTCAATCCATGGAAAGGGAGAATGGATGACCACTGAAGAC ATGCTTGTGGTGTGGAACAGAGTGTGGATTGAGGAGAACGACCACATGGAA GACAAGACCCCAGTTACGAAATGGACAGACATTCCCTATCTGGGAAAAAGG GAAGACTTGTGGTGTGGATCTCTCATAGGGCACAGACCGCGCACCACCTGGG CTGAGAACATTAAAAACACAGTCAACATGGTGCGCAGGATCATAGGTGATGA AGAAAAGTACATGGACTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAA GGGTCTACACCTGGAGTGCTATAAGCACCAATCTTAGTGTTGTCAGGCCTGCT AGTCAGCCACAGCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGA GGCTGGGAAACCAAGCCCATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCAC GCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTT CAATCTGGGGCCTGAACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTA GTGGTTAGAGGAGACCCCCCGGAAAACGCAAAACAGCATATTGACGCTGGG AAAGACCAGAGACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGAT CGCCGAATAGCGGCGGCCGGTGTGGGGAAATCCATGGGTCT
SEQ ID NO：8
KU681082.3Zika病毒分离株Zika病毒/H.sapiens-tc/PHL/2012/CPC-0740，菲律宾，完整的基因组
AGTTGTTGATCTGTGTGAATCAGACTGCGACAGTTCGAGTTTGAAGCGA AAGCTAGCAACAGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTC TGGTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATAT GCTAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCT GCCAGCCGGACTTCTGCTGGGCCATGGGCCCATCAGGATGGTCTTGGCGATA CTAGCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAG ATGGGGTTCAGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAA GAAAGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAA GAGACGAGGCGCAGATACTAGCGTCGGAATTGTTGGCCTCCTCCTGACCACA GCCATGGCAGTAGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGA CAGAAGCGATGCTGGGGAGGCCATATCTTTTCCAACCACACTGGGGATGAAT AAGTGTTACATACAAATCATGGATCTTGGACACATGTGTGATGCCACCATGAG CTATGAATGCCCTATGTTGGATGAGGGGGTAGAACCAGATGACGTCGATTGCT GGTGCAACACGACATCAACTTGGGTTGTGTATGGAACCTGCCACCACAAAAA AGGTGAAGCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACT AGGAAGCTGCAAACGCGGTCGCAGACCTGGTTGGAATCAAGAGAATACACA AAGCACCTGATTAGAGTTGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTT AGCAGCAGCTGTCATCGCTTGGCTTTTGGGAAGTTCAACGAGCCAAAAAGT CATATATCTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCAT AGGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTT GATGTTGTCTTGGAACATGGAGGTTGTGTTACCGTAATGGCACAGGACAAAC CGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGT AAGATCCTACTGCTATGAGGCATCAATATCGGATATGGCTTCGGACAGCCGCT GCCCAACACAAGGTGAGGCCTACCTTGACAAGCAGTCAGACACTCAATATGT CTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTT TGGCAAAGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATG ACCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAG TTCATGGCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAAC TGATGAGAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCCGA AGCCACCCTGGGGGGTTTTGGGAGCCTAGGACTTGATTGTGAACCGAGGACAGGCCTTGACTTTTCAGATTTGTATTACCTGACTATGAATAACAAGCACTGGT TGGTTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCATGCTGGGGC AGACACTGGAACTCCACATTGGAACAACAAAGAAGCACTGGTAGAGTTCAA GGACGCACATGCAAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAAGG AGCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGAGC CAAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAA CTTAGATTGAAGGGCGTGTCATACTCCTTGTGCACTGCAGCGTTCACATTCAC CAAGATCCCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTA CGCAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGATATG CAAACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCTGTAATCACTG AAAGCACCGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGA CTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCATCACTGGCAC AGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGG GGTGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGCTTT CAAATCATTGTTCGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGT TGCTGGTGTGGTTGGGTCTGAATACAAAGAATGGATCTATTTCCCTTACGTGC TTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTTTCTGCTGATGT GGGGTGCTCGGTGGACTTCTCAAAGAAGGAAACGAGATGCGGTACAGGGGT GTTCGTCTATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCATCCT GACTCCCCTCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATGGG ATCTGTGGGATCTCCTCTGTCTCAAGAATGGAAAACATCATGTGGAGATCAGT AGAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACGGT CGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCCC GTGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTAC TTCGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACACAC TGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGA TCATGGGTTTGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATT ATTCATTAGAGTGTGATCCAGCCGTCATTGGAACAGCTGCTAAGGGAAAGGA GGCTGTGCACAGCGATCTAGGCTACTGGATTGAGAGTGAGAAGAACGACAC ATGGAGGCTGAAGAGGGCCCACCTGATCGAGATGAAAACATGTGAATGGCC AAAGTCCCACACATTGTGGACAGATGGAGTAGAAGAAAGTGATCTGATCATA CCCAAGTCTTTAGCTGGGCCACTCAGCCATCACAACACCAGAGAGGGCTACA GGACTCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTG AGGAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGGACAAGAG GACCATCCCTGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGT GCTGCAGGGAATGCACAATGCCCCCACTGTCGTTCCGAGCTAAAGATGGCTG TTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGTAACTTAGTA AGGTCAATGGTGACTGCAGGATCAACTGATCACATGGATCACTTCTCTCTTGG AGTGCTTGTGATTTTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATGACC ACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCCATGATCCTGG GAGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCCACC TTCGCGGAAATGAACACTGGAGGAGATGTAGCTCATTTGGCGCTGATAGCGG CATTCAAAGTCAGACCTGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGG ACACCCCGTGAGAGCATGCTGCTGGCCTTGGCCTCGTGTCTTCTGCAAACTG CGATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGACAACATCACCT TGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTGGC GTGGAGAGCAGGCCTTGCTACTTGCGGGGGGTTCATGCTCCTCTCTCTGAAG GGGAAAGGCAGTGTGAAGAAGAACCTACCATTTGTCATGGCCTTGGGACTA ACTGCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCA CAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGTGAAGTACTCACAGCTGTTG GCCTGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCGGATATAGAGATGGC TGGGCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGA AAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAA GATGCGGAAATCACTGGAAACAGTCCCCGGCTCGATGTGGCACTAGATGAGA GTGGTGATTTCTCCCTAGTGGAGGATGATGGTCCACCCATGAGAGAGATCATA CTCAAAGTGGTCCTGATGACCATCTGCGGCATGAACCCAATAGCCATACCCTT TGCAGCTGGAGCGTGGTACGTGTATGTGAAGACTGGAAAAAGGAGTGGTGC TCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGA TGGAGTGTACAGAGTAATGACTCGTAGACTGCTTGGTTCAACACAAGTTGGA GTGGGAGTCATGCAAGAGGGGGTCTTCCACACTATGTGGCACGTCACAAAA GGATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGAT GTCAAGCAGGATCTGGTGTCATACTGTGGTCCGTGGAAGCTAGACGCCGCCT GGGACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGA GCGAGGAACATCCAGACTCTGCCCGGAACATTTAAGACAAAGGATGGGGAC ATTGGAGCAGTTGCGCTGGACTACCCAGCAGGAACTTCAGGATCTCCAATCC TAGACAAGTGTGGGAGAGTGATAGGACTCTATGGTAATGGGGTCGTGATAAA AAATGGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAGAC TCCTGTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTC TTAGACCTGCATCCTGGAGCCGGGAAAACCAGGAGAGTTCTTCCTGAAATAG TCCGTGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTCGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTTCGTTAT ATGACAACAGCAGTCAATGTCACCCATTCTGGGACAGAAATCGTTGACTTAA TGTGCCATGCTACCTTCACTTCACGCCTACTACAACCAATCAGAGTCCCCAAC TATAATTTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCA GCAAGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCATCT TCATGACCGCCACGCCACCAGGAACCCGTGACGCATTCCCGGACTCCAACTC ACCAATTATGGACACCGAGGTGGAAGTCCCAGAGAGAGCCTGGAGCACAGG CTTTGATTGGGTGACGGATCATTCTGGGAAAACAGTCTGGTTTGTTCCAAGC GTGAGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGG GTCATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACGAAA AATCAAGAGTGGGACTTCGTCGTGACAACCGACATTTCAGAGATGGGCGCC AACTTTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCTTAAAGCCGGTCAT ACTTGATGGCGAGAGAGTCATTTTGGCTGGACCCATGCCTGTCACACATGCC AGCGCTGCTCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGA GATGAGTATCTGTATGGAGGTGGGTGCGCAGAGACTGATGAAGATCACGCAC ACTGGCTTGAAGCAAGAATGCTTCTTGACAACATTTACCTCCAAGATGGCCT CATAGCTTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCTATTGAGGGA GAGTTCAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGAAA AGAGGAGATCTTCCGGTTTGGTTGGCCTATCAGGTTGCATCTGCCGGAATAAC CTACACAGATAGAAGATGGTGCTTTGATGGCATGACCAACAACACCATAATG GAAGACAGTGTGCCGGCAGAGGTGTGGACCAGATACGGAGAGAAAAGAGT GCTCAAACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTG AAGTCATTCAAAGAGTTTGCCGCTGGGAAAAGAGGAGCGGCCTTTGGAGTG ATAGAAGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGGAA GCCATTGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTT ACAAAGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTT GGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGCGGAAC AAGGGCATGGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCA TGGCTTATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCCTCAT TGTCGTGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGA TCTCCTCAGGACAACCAAATGGCAATCATCATCATGGTAGCAGTGGGTCTTCT GGGCTTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAAAGTGA CCTAAGCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCACAGGATTCTC AATGGACATTGACCTGCGGCCAGCCTCAGCTTGGGCTATCTATGCTGCTCTGA CAACTTTCATCACCCCAGCCGTCCAACATGCGGTGACCACTTCATACAACAA CTACTCCTTAATGGCGATGGCCACGCAAGCTGGGGTGTTGTTTGGTATGGGCA AAGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATGGGT TGCTACTCACAATTAACACCTCTGACCCTAATAGTGGCCATCATTTTGCTCGT GGCGCACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGGGCT GCCCAGAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGATGGA ATAGTGGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAAAAAA AGATGGGGCAGGTGCTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTC GCGGACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCTGCAAC TTCCACCTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCCACAGCC ACTTCACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCTAAT CTACACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACGGGAGAGACCCTGGGAGAGAAATGGAAGGCCCGCCTGAACCAGATGTCGGC CCTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGA AGAGGCCCGCCGTGCCCTCAAGGACGGTGTGGCAACAGGAGGCCATGCTGT GTCCCGAGGAAGTGCAAAGCTTAGATGGCTGGTGGAGAGAGGATACCTGCA GCCCTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTAC TATGCCGCCACCATCCGCAAAGTTCAGGAAGTGAAAGGATACACAAAAGGA GGCCCTGGTCATGAAGAACCCATGTTGGTGCAAAGCTATGGGTGGAACATAG TCCGTCTTAAGAGTGGGGTGGACGTCTTTCACATGGCGGCTGAGCCGTGTGA CACTTTGCTGTGTGATATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAG CACGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGAC CAGGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAA ACCCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGGGTGCCA CTCTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGA CGGGCCCAGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGC ACGCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTA ACCGCATTGAGAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACG AGAACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCTAC ACAAGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAA CCCTGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACTGACACCACAC CGTATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAG ACCCCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTATG GAAGGAGCTAGGCAAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTT CATCAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAA AAAGAGTGGAAGACTGCAGTGGAAGCTGTGAATGATCCAAGGTTCTGGGCT CTAGTGGACAAGGAAAGAGAGCATCACCTGAGAGGAGAGTGTCAGAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAA GGCCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTCCTA GAGTTCGAAGCCCTTGGATTCTTGAATGAGGATCATTGGATGGGGAGAGAGA ATTCAGGAGGTGGTGTTGAAGGACTGGGATTACAAAGACTCGGATATGTCCT AGAAGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGATACTGCT GGCTGGGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCA CCAACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTA CACATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAA GACAGTTATGGACATTATTTCAAGACAAGACCAAAGGGGGAGCGGACAAGT TGTCACTTACGCTCTTAATACATTCACCAACCTGGTGGTGCAGCTCATTCGGA ATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTGCGGAG GCCAGAGAAAGTGACCAACTGGTTGCAAAGCAACGGATGGGATAGGCTCAA AAGAATGGCAGTCAGTGGAGATGATTGCGTTGTGAAACCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACA CACAAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGT TTTGCTCCCACCACTTCAACAAACTCCATCTTAAGGACGGGAGGTCCATTGT GGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGAGCCCGCGTATCACCA GGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGC AAATGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGGCCAA TGCCATTTGTTCATCTGTGCCAGTTGATTGGGTTCCAACTGGGAGAACTACCT GGTCAATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGT ATGGAACAGAGTGTGGATTGAGGAAAACGACCACATGGAAGACAAGACCCC AGTTACAAAATGGACAGACATTCCCTATTTGGGAAAAAGAGAAGACTTGTGG TGTGGATCTCTCATAGGGCACAGACCGCGTACTACCTGGGCTGAGAACATCA AAAATACAGTCAACATGATGCGCAGGATCATAGGTGATGAAGAAAAGTACAT GGACTACCTATCCACCCAGGTTCGCTACTTGGGTGAAGAAGGGTCCACACCT GGAGTGCTGTAAGCACCAATCTTAGTGTTGTCAGGCCTGCTAGTCAGCCACA GCTTGGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAAAC CAAGCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGC CTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGG CGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGC CTGAACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAG GAGACCCCCCGGAAAACGCAAAACAGCATATTGACGCTGGGAAAGACCAGA GACTCCATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAATAGC GGCGGCCGGTGTGGGGAAATCCATGGGTCT
SEQ ID NO：9
KU707826.1Zika病毒分离株SSABR1，巴西，完整的基因组
GACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAACAGGTTTTA TTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAAAAAAGAAATC CGGAGGATTCCGGATTGTCAATATGCTAAAACGCGGAGTAGCCCGTGTGAGC CCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTGGGTCATGGGC CCATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCACGGCAATCAAG CCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAAAAAGAGGCTA TGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGCTGAGAATAAT CAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTAGTGTCGGAAT TGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCACTAGACGTGGG AGTGCATACTATATGTACTTGGACAGAAACGATGCTGGGGAGGCCATATCTTT TCCAACCACATTGGGGATGAATAAGTGTTATATACAGATCATGGATCTTGGAC ACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGATGAGGGGGTG GAACCAGATGACGTCGATTGTTGGTGCAACACGACGTCAACTTGGGTTGTGT ACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCTAGAAGAGCTG TGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGTCGCAAACCTG GTTGGAATCAAGAGAATACACAAAGCACTTGATTAGAGTCGAAAATTGGATA TTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTTGGCTTTTGG GAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACTGCTGATTGCC CCGGCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGACTTTGTGGAAG GTATGTCAGGTGGGACCTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTGT CACCGTAATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACA ACAGTCAGCAACATGGCGGAGGTAAGATCCTACTGCTATGAGGCATCAATATC AGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAGCCTACCTTGA CAAGCAATCAGACACTCAATATGTCTGCAAAAGAACGTTAGTGGACAGAGG CTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGTGACATGCGCT AAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAGCCAGAGAAT CTGGAGTACCGGATAATGCTGTCAGTTCATGGCTCCCAGCACAGTGGGATGA TTGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAAAGTTGAGAT AACGCCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTTGGAAGCCT AGGACTTGATTGTGAACCGAGGACAGGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTCCACGACAT TCCATTACCTTGGCACGCTGGGGCAGACACCGGAACTCCACACTGGAACAA CAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAAAAGGCAAACTGT CGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTTGCTGGAGC TCTGGAGGCTGAGATGGATGGTGCAAAGGGAAGGCTGTCCTCTGGCCACTT GAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGTCATACTCCT TGTGTACTGCAGCGTTCACATTCACCAAGATCCCGGCTGAAACACTGCACGG GACAGTCACAGTGGAGGTACAGTACGCAGGGACAGATGGACCTTGCAAGGT TCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTTGGGAGGTTG ATAACCGCTAACCCCGTAATCACTGAAAGCACTGAGAACTCTAAGATGATGC TGGAACTTGATCCACCATTTGGGGACTCTTACATTGTCATAGGAGTCGGGGA GAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATTGGAAAAGC ATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGGAGACAC AGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGGGCAAGGGC ATCCATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGGAATGTCCTG GTTCTCACAAATTCTCATTGGAACGTTGCTGATGTGGTTGGGTCTGAACACA AAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTGTTGATCTT CTTATCCACAGCCGTCTCTGCTGATGTGGGGTGCTCGGTGGACTTCTCAAAG AAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATAACGACGTTGAAGCCT GGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGATTGGCAGCAGC AGTCAAGCAAGCCTGGGAAGATGGTATCTGCGGGATCTCCTCTGTTTCAAGA ATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGCAATCCTGG AAGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCAT GTGGAGAGGTCCACAGAGATTGCCCGTGCCTGTGAACGAGCTGCCCCACGG CTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAGACAAATAA CAGCTTTGTCGTGGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGA GCATGGAACAGCTTTCTTGTGGAGGATCATGGGTTCGGGGTATTTCACACTAG TGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCCAGCCGTTA TTGGAACAGCTGTTAAGGGAAAGGAGGCTGTACACAGTGATCTAGGCTACTG GATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGCCCATCTGAT CGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGACAGATGG AATAGAAGAGAGTGATCTGATCATACCCAAGTCTTTAGCTGGGCCACTCAGC CATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCCATGGCAC AGTGAAGAGCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAGGTCCAC GTGGAGGAAACATGTGGAACAAGAGGACCATCTCTGAGATCAACCACTGCA AGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATGCCCCCA CTGTCGTTCCGGGCTAAAGATGGCTGTTGGTATGGAATGGAGATAAGGCCCA GGAAAGAACCAGAAAGCAACTTAGTAAGGTCAATGGTGACTGCAGGATCAA CTGATCACATGGACCACTTCTCCCTTGGAGTGCTTGTGATTCTGCTCATGGTG CAGGAAGGGCTGAAGAAGAGAATGACCACAAAGATCATCATAAGCACATCA ATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGACCTGGC TAAGCTTGCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACTGGAGGA GATGTAGCTCATCTGGCGCTGATAGCGGCATTCAAAGTCAGACCAGCGTTGC TGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATGCTGCTG GCCTTGGCCTCGTGTCTTTTGCAAACTGCGATCTCCGCCTTGGAAGGCGACC TGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATG GTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGCTCTGACACC ACTGGCCCGGGGCACACTGCTTGTGGCGTGGAGAGCAGGCCTTGCTACTTGC GGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAGAAGAAC TTACCATTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCGACCCCAT CAACGTGGTGGGACTGCTGTTGCTCACAAGGAGTGGGAAGCGGAGCTGGCC CCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCTGGAGGG TTCGCCAAGGCAGATATAGAGATGGCTGGGCCCATGGCCGCGGTCGGTCTGC TAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGACATGTACATTGAAAG AGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAACAGTCC CCGGCTCGATGTGGCGCTAGATGAGAGTGGTGATTTCTCCCTGGTGGAGGAT GACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGACCATCT GTGGCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACGTATAC GTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGT AGACTGCTAGGTTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGGGGTC TTTCACACTATGTGGCACGTCACAAAAGGATCCGCGCTGAGAAGCGGTGAA GGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCATACT GTGGTCCATGGAAGCTAGATGCCGCCTGGGACGGGCACAGCGAGGTGCAGC TCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACTCTGCCCG GAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATTACCC AGCAGGAACTTCAGGATCTCCAATCCTAGACAAGTGTGGGAGAGTGATAGG ACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCATCA CCCAAGGGAGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTCGA TGCTGAAGAAGAAGCAGCTAACTGTCTTAGACTTGCATCCTGGAGCTGGGAA AACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGACTC CGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAGG CCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGTCAATGTCACCCA CTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCACGTC TACTACAGCCAATCAGAGTCCCCAACTATAATCTGTATATTATGGATGAGGCCC ACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAAGGGT TGAGATGGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGGAAC CCGTGACGCATTTCCGGACTCCAACTCACCAATTATGGACACCGAAGTGGAA GTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATCATTCTG GAAAAACAGTTTGGTTTGTTCCAAGCGTGAGGAACGGCAATGAGATCGCAG CTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCAGCAGAAAGACTT TTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGA CAACTGACATTTCAGAGATGGGCGCCAACTTTAAAGCTGACCGTGTCATAGA TTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATTCTG GCTGGACCCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGATGAGTATCTGTATGGAGGTGGGT GCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGCTCC TTGACAATATTTACCTCCAAGATGGCCTCATAGCCTCGCTCTATCGACCTGAG GCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTTAGGACGGAGCAA AGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCTGG CCTATCAGGTTGCATCTGCCGGAATAACCTACACAGATAGAAGATGGTGCTTT GATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAGGTG TGGACCAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGACGC CAGAGTTTGTTCAGATCATGCGGCCCTGAAGTCATTCAAGGAGTTTGCCGCT GGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGCCA GGACACATGACAGAGAGATTCCAGGAAGCCATTGACAACCTCGCTGTGCTCA TGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGCGGCGGCCCAATTGC CGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTCGCTGGGAATCTTCTTCGTCTTGATGAGGAACAAGGGCATAGGGAAGATGGGCTTT GGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGAAATTG AGCCAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTTCTATTGCTGGTGGTG CTCATACCTGAGCCAGAAAAGCAAAGATCTCCCCAGGACAACCAAATGGCA ATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGAACT CGGATGGTTGGAGAGAACAAAGAGTGACCTAAGCCATCTAATGGGAAGGAG AGAGGAGGGGGCAACCATAGGATTCTCAATGGACATTGACCTGCGGCCAGCC TCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCGTCCA ACATGCAGTGACCACTTCATACAACAACTACTCCTTAATGGCGATGGCCACGC AAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACACCCCTGA CCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTACATGTACTTGATCCCA GGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCTGG CATCATGAAGAACCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACACA ATGACAATTGACCCCCAAGTGGAGAAAAAGATGGGACAGGTGCTACTCATAG CAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGGGG AGGCTGGGGCCCTGATCACAGCCGCAACTTCCACTTTGTGGGAAGGCTCTCC GAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAACATTTTTAGG GGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGCTG GCTTGGTCAAGAGACGTGGGGGTGGAACAGGAGAGACCCTGGGAGAGAAA TGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAA AGTCAGGCATCACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAAGG ACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGATCT TGGATGTGGCAGAGGGGGCTGGAGTTACTACGCCGCCACCATCCGCAAAGTT CAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATGAAGAACCCGTG TTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGACG TCTTTCATATGGCGGCTGAGCCGTGTGACACGTTGCTGTGTGACATAGGTGA GTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTCTCC ATGGTGGGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAGGTGT TGTGCCCATACACCAGCACTATGATGGAAACCCTGGAGCGACTGCAGCGTAG GTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGAG ATGTATTGGGTCTCTGGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACCA CGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCTAGGAGGCCAGTGAAATA TGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCGCT GAAGCTCCCAACATGAAGATCATTGGTAACCGCATTGAAAGGATCCGCAGTG AGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGGACATGGGC TTACCATGGAAGCTATGAGGCCCCCACACAAGGGTCAGCGTCCTCTCTAATA AACGGGGTTGTCAGGCTCCTGTCAAAACCCTGGGATGTGGTGACTGGAGTC ACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTCA AGGAAAAAGTGGACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCAG GTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAGCTAGGCAAACACAAAC GGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATGC AGCATTAGGGGCAATATTTGAAGAGGAAAAAGAGTGGAAGACTGCAGTGGA AGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGATAAGGAAAGAGAGCA CCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAAG AGAAAAGAAACAAGGGGAATTTGGAAAGGCCAAGGGCAGCCGCGCCATCT GGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTTG AACGAGGATCACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAGAGATGAGTCGTATACCA GGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAGC AGGTTTGATCTGGAGAATGAAGCTCTAATCACCAACCAAATGGAAAAAGGGC ACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAAACAAAGTGGT AAAGGTCCTTAGACCAGCTGAAAAAGGGAAAACAGTTATGGACATTATTTCG AGACAAGACCAAAGGGGGAGCGGACAAGTTGTCACTTACGCTCTTAACACA TTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTTCT AGAGATGCAAGACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAACTG GTTGCAGAGCAACGGATGGGATAGGCTCAAACGAATGGCAGTCAGTGGAGA TGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTTCT TGAATGATATGGGAAAAGTTAGGAAGGACACACAAGAGTGGAAACCCTCAA CTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCCACCACTTCAACAA GCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCAGGGGCGGGATGGAGCATCCGG GAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTATTT CCACAGAAGGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCCA GTTGACTGGGTTCCAACTGGGAGAACTACCTGGTCAATCCATGGAAAGGGA GAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATTG AGGAGAACGACCACATGGAAGACAAGACCCCAGTTACGAAATGGACAGACA TCCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCTCTCATAGGGCA CAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGGT GCGCAGGATCATAGGTGATGAAGAAAAGTACATGGACTACCTATCCACCCAA GTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCACCAG TCTTAATGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGCA GCCTGTGACCCCCCCAGGAGAAGCTGGGAAACCAAGCCTATAGTCAGGCCG AGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAA GGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGGAGATCAGCTG TGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAG
SEQ ID NO：10
KU744693.1Zika病毒分离株VE_Ganxian，中国，完整的基因组
GTTGTTACTGTTGCTGACTCAGACTGCGACAGTTCGAGTTTGAAGCGAA AGCTAGCAACAGTATCAACAGGTTTTATTTGGATTTGGAAACGAGAGTTTCTG GTCATGAAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGC TAAAACGCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGC CAGCCGGACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCAATTCTA GCCTTTTTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATG GGGTTCAGTGGGGAAAAAAGATGCTATGGAAATAATAAAGAAGTTCAAGAA AGATCTGGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAAGAG ACGAGGCGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCT ATGGCAGCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGACA GAAACGATGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAA GTGTTATATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTA TGAATGCCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGG TGCAACACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAA GGTGAAGCACGGAGATCTAGAAGAGCTGTGACGCTCCCTTCCCATTCCACTA GGAAGCTGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAA AGCACTTGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTA GCAGCAGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAGTC ATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATA GGAGTCAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTG ATGTTGTCTTGGAACATGGAGGTTGTGTCACCGCAATGGCACAGGACAAACC GACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGT AAGATCCTACTGCTATGAGGCATCAATATCAGACATGGCTTCGGACAGCCGCT GCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGT TTGCAAAAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTT GGCAAAGGGAGTCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATGA CCGGGAAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAGT TCATGGCTCCCAGCACAGTGGGATGCTCGTTAATGACACAGGACATGAAACT GATGAGAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCCGAA GCCACCCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACA GGCCTTGACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTT GGCTCACAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGGGCA GCCACCGGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAG GACGCACATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAAGGA GCAGTTCACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGCA AAGGGAAGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAAC TTAGATTGAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCACATTCACC AAGATCCCGGCTGAAACAGTGGACGGGACAGTCACAGTGGAGGGACAGTAC GGAGGGACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATG CAGACTCTGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCACTG AAAGCACTGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGA CTCTTACATTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCAC AGGAGTGGCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGA GGCGCTCTCAACTCATTGGGCAAGGGCATCCATCAAATTATTGGAGCAGCTTT CAAATCATTGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGGACGT TGCTGATGTGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGC TTGGCCTTAGGGGGAGTGTTGATCTTCTTATCCACAGCCGTCTCAGGTGGTGT GGGGTGCTCGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGT GTTCGTCTATAACGATGTTGAAGCCTGGAGGGACAGGTACAAGTACCATCCT GACTCCCCCCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATGGTA TCTGCGGGATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTA GAAGGGGAGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCCCG TGCCTGTGAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTT CGTCAGAGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACACACT GAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGAT CATGGGTTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGACT ATTGGTTAGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAGGA GGCTGTACACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACA TGGTGGCTGAAGAGGGCCCATCTGATCGAGATGAAAACATGTGAATGGCCAA AGTCCCACACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATACC CAAGTCTTTAGCTGGGCCACTCAGCCATCACAATGCCAGAGAGGGCTACAGG ACCCAAATGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTGAG GAATGCCCAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGA CCATCTCTGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTCCAGGGAGTGCACAATGCCCCCACTGTCCTTCCAGGCTAAAGATGGCTGTTG GTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGCAACTTAGTAAG GTCAATGGTGACTGCAGGATCAACTGATCACATGGATCACTTCTCCCTTGGA GTGCTTGTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATGACC ACAAAGATCATCATAAGCACATCAATGGCAGTGCTGGTAGCTATGATCCTGGG AGGATTTTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCCACCT TCGCGGAAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGC ATTCAAAGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGA CACCCCGTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAACTGC GATCTCCGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGAGCGATGGTTGTTCCACGCACTGATAACATCACCTTA GCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTGGCGT GGAGAGCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGG AAAAGGCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGACTAACC GCTGTGAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCACAA GGAGTGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCC TGATATGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATGGCTGG GCCCATGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAG AGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAAGAT GCGGAAGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATGAGAGT GGTGATTTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATAC TCAAGGTGGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATACCCTTT GCAGCTGGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCT CTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGAT GGAGTGTACAGAGTAATGACTCGCAGACTGCTAGGTTCAACACAAGTTGGA GTGGGAGTTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAG GATCCGCGCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGT CAAGCAGGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCGCCTGG GACGGGCACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCG AGGAACATCCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGGACATTG GAGCGGTTGCACTGGATTACCCAGCAGGAACTTCAGGATCTCCAATCCTAGA CAAGTGTGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAAAAAT GGGAGTTATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAGACTCCT GTTGAGTGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAG ACTTGCATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCG TGAAGCCATAAAAACAAGACTCCGTACTGTGATCTTGGCTCCAACCAGGGTT GTCGCTGCTGAAATGGAGGAGGCCCTTAGAGGGCTTCCAGTGCGTTATATGA CAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTTAATGTG CCATGCCACCTTCACTTCACGTCTACTACAGCCAATTAGAGTCCCCAACTATA ATCTGTATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCA AGAGGATACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCATCTTCA TGACCGCCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCACC AATTATGGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTT TGATTGGGTGACGGAGTATTCTGGAAAAACAGTTTGGTTTGTTCCACGCGTG AGGAACGGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTC ATACAGCTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCGCCAACT TTAAAGCTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTT GGTGGCGAGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATGCCAGCG CTGCCCAGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGATG AGTATCTGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTG GCTTGAAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGCCTCATAG CCTCGCTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTT CAAGCTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGG AGATCTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATAACCTACA CAGATAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGA CAGTGTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGAGTGCTCA AACCGAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGAAGTC ATTCAAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGA AGCCCTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGGAAGCCAT TGACAACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAA AGCCGCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGG TTGCTGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGAGGAACAAGG GCATAGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCT CATGTGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCCTCATTGTTG TGTTCCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGATCTCC CCAGGACAACCAAATGGCCATCATCATCATGGTAGCAGTAGGTCTTCTGGGC TTGATTACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAGTGACCTAA GCCATCTAATGGGAAGGAGAGAGGAGGGGGCAACCATGGGATTCTCAATGG ACATTGACCTGCGGCCAGCCTCAGCTTGGGCCATCTATCCTGCCTTGACATCT TTCATTACCCCAGCCGTCCAACATGCAGTGACCACTTCATACAACAACTACTC CTTAATGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGG ATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTTGCTA CTCACAATTAACGCCCCTGACCCTAATAGTGGCCATCATTTTGCTCGTGGCGC ACTACATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCA GAAGAGAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGAGGGAATAGT GGTGACTGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGAT GGGACAGGTGCTACTCATGGCAGTAGCCGTCTCCAGCGCCATACTGTCGAGG ACCGCCTGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCCGCAACTTCC ACTTTGTGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACCT CACTGTGTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCTAATCTAC ACAGTAACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACAGG AGAGACCCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCT GGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGAAGA GGCCCGCCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTC CCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCC CTATGGAAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTACTAC GCCGCCACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGC CCTGGTCATGAAGAACCCGTGTTGGTGCAAAGCTATGGGTGGAACATAGTCC GTCTTAAGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGTGACAC GTTGCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCA CGGACGCTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGACCA GGAGCCTTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAAAC CCTGGAGCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACT CTCCCGCAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCAAC ACCATAAAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACG GGCCTAGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCAC GCGGGCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTAAC CGCATTGAAAGGATCCGCGCTGAGAAAGCGGAAACGTGGTTCTTTGACGAG AACCACCCATATAGGACATGGGCTTACCATGGAAGCTATGATGCCGCCACACA AGGGTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAACCC TGGGATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGT ATGGTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAGACC CCCAAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAA AGAGCTAGGCAAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCAT CAACAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAAAAA GAGTGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTA GTGGACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGAGTTGTGT GTACATCACAATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAAGG CCAAGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAG TTCGAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGAACT CAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGA AGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGC TGGGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCACCA ACCAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACAC ATACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGAC AGTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGACAAGTTGTC ACTTACGCTCTCAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTGCGGAGGTC AGAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACG AATGGCGGTCAGTGGAGATGATTGCGTTGTGAAACCAATTGATGATAGGTTT GCACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACACAC AAGAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCCTTCT GCTCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGT TCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCAGGG GCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAA TGTGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGGCCAATGC CATTTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACCTGGT CAATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGCGTG GAACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAGACCCCAGT CACGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAA ACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGA CTACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGA GTGCTGTAAGCACCAATCTTAATGTTGTCAGGCCTGCTAGTCAGCCACAGCTT GGGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAAACCAA GCCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGT GAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGGCGCA GGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGA ACTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGA SEQ ID NO：11
LC002520.1Zika病毒基因组RNA，菌株：MR766-NIID，乌干达，完整的基 因组
AGTTGTTGATCTGTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGA GAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTC TGGTCATGAAAAACCCAAAGAAGAAATCCGGAGGATTCCGGATTGTCAATAT GCTAAAACGCGGAGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAGGTT GCCAGCCGGACTTCTGCTGGGTCATGGACCCATCAGAATGGTTTTGGCGATA CTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCCTTATCAACAG ATGGGGTTCCGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAA GAAAGATCTTGCTGCCATGTTGAGAATAATCAATGCTAGGAAAGAGAGGAAG AGACGTGGCGCAGACACCAGCATCGGAATCATTGGCCTCCTGCTGACTACAG CCATGGCAGCAGAGATCACTAGACGCGGGAGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAAC AAGTGCCACGTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGA GTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTG CTGGTGCAACACGACATCAACTTGGGTTGTGTACGGAACCTGTCATCACAAA AAAGGTGAGGCACGGCGATCTAGAAGAGCCGTGACGCTCCCTTCTCACTCTA CAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCAAGAGAATACA CGAAGCACTTGATCAAGGTTGAAAACTGGATATTCAGGAACCCCGGGTTTGC GCTAGTGGCCGTTGCCATTGCCTGGCTTTTGGGAAGCTCGACGAGCCAAAAA GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGTATCAGGTGC ATTGGAGTCAGCAATAGAGACTTCGTGGAGGGCATGTCAGGTGGGACCTGG GTTGATGTTGTCTTGGAACATGGAGGCTGCGTTACCGTGATGGCACAGGACA AGCCAACAGTTGACATAGAGTTGGTCACGACGACGGTTAGTAACATGGCCGA GGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTTCGGACAGTC GTTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATA TGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACT TTTTGGCAAAGGGAGCTTGGTGACATGTGCCAAGTTTACGTGTTCTAAGAAG ATGACCGGGAAGAGCATTCAACCGGAAAATCTGGAGTATCGGATAATGCTAT CAGTGCATGGCTCCCAGCATAGCGGGATGACTGTCAATGATATAGGATATGAA ACTGACGAAAATAGAGCGAAAGTCGAGGTTACGCCTAATTCACCAAGAGCG GAAGCAACCTTGGGAGGCTTTGGAAGCTTAGGACTTGACTGTGAACCAAGG ACAGGCCTTGACTTTTCAGATCTGTATTACCTGACCATGAACAATAAGCATTG GTTGGTGCACAAAGAGTGGTTTCATGACATCCCATTGCCTTGGCATGCTGGG GCAGACACTGGAACTCCACACTGGAACAACAAAGAGGCATTGGTAGAATTCAAGGATGCCCACGCCAAGAGGCAAACCGTCGTCGTTCTGGGGAGCCAGGAA GGAGCCGTTCACACGGCTCTCGCTGGAGCTCTAGAGGCTGAGATGGATGGTG CAAAGGGAAAGCTGTTCTCTGGCCATTTGAAATGCCGCCTAAAAATGGACAA GCTTAGATTGAAGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACATTCA CCAAGGTCCCAGCTGAAACACTGCATGGAACAGTCACAGTGGAGGTGCAGT ATGCAGGGACAGATGGACCCTGCAAGATCCCAGTCCAGATGGCGGTGGACAT GCAGACCCTGACCCCAGTTGGAAGGCTGATAACCGCCAACCCCGTGATTACT GAAAGCACTGAGAACTCAAAGATGATGTTGGAGCTTGACCCACCATTTGGG GATTCTTACATTGTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGC ATAGGAGTGGTAGCACCATCGGAAAGGCATTTGAGGCCACTGTGAGAGGCG CCAAGAGAATGGCAGTCCTGGGGGATACAGCCTGGGACTTCGGATCAGTCG GGGGTGTGTTCAACTCACTGGGTAAGGGCATTCACCAGATTTTTGGAGCAGC CTTCAAATCACTGTTTGGAGGAATGTCCTGGTTCTCACAGATCCTCATAGGCACGCTGCTAGTGTGGTTAGGTTTGAACACAAAGAATGGATCTATCTCCCTCACA TGCTTGGCCCTGGGGGGAGTGATGATCTTCCTCTCCACGGCTGTTTCTGCTGA CGTGGGGTGCTCAGTGGACTTCTCAAAAAAGGAAACGAGATGTGGCACGGG GGTATTCATCTATAATGATGTTGAAGCCTGGAGGGACCGGTACAAGTACCATC CTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGCAGGCCTGGGAAGAGG GGATCTGTGGGATCTCATCCGTTTCAAGAATGGAAAACATCATGTGGAAATC AGTAGAAGGGGAGCTCAATGCTATCCTAGAGGAGAATGGAGTTCAACTGACA GTTGTTGTGGGATCTGTAAAAAACCCCATGTGGAGAGGTCCACAAAGATTGC CAGTGCCTGTGAATGAGCTGCCCCATGGCTGGAAAGCCTGGGGGAAATCGTA TTTTGTTAGGGCGGCAAAGACCAACAACAGTTTTGTTGTCGACGGTGACACA CTGAAGGAATGTCCGCTTGAGCACAGAGCATGGAATAGTTTTCTTGTGGAGG ATCACGGGTTTGGAGTCTTCCACACCAGTGTCTGGCTTAAGGTCAGAGAAGA TTACTCATTAGAATGTGACCCAGCCGTCATAGGAACAGCTGTTAAGGGAAGG GAGGCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGAC ACATGGAGGCTGAAGAGGGCCCACCTGATTGAGATGAAAACATGTGAATGG CCAAAGTCTCACACATTGTGGACAGATGGAGTAGAAGAAAGTGATCTTATCA TACCCAAGTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTA CAGAACCCAAGTGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATCCGGTT TGAGGAATGTCCAGGCACCAAGGTTTACGTGGAGGAGACATGCGGAACTAG AGGACCATCTCTGAGATCAACTACTGCAAGTGGAAGGGTCATTGAGGAATGG TGCTGTAGGGAATGCACAATGCCCCCACTATCGTTTCGAGCAAAAGACGGCT GCTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAG TGAGGTCAATGGTGACAGCGGGGTCAACCGATCATATGGACCACTTCTCTCT TGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAGAGAATG ACCACAAAGATCATCATGAGCACATCAATGGCAGTGCTGGTAGTCATGATCTT GGGAGGATTTTCAATGAGTGACCTGGCCAAGCTTGTGATCCTGATGGGTGCT ACTTTCGCAGAAATGAACACTGGAGGAGATGTAGCTCACTTGGCATTGGTAG CGGCATTTAAAGTCAGACCAGCCTTGCTGGTCTCCTTCATTTTCAGAGCCAAT TGGACACCCCGTGAGAGCATGCTGCTAGCCCTGGCTTCGTGTCTTCTGCAAA CTGCGATCTCTGCTCTTGAAGGTGACTTGATGGTCCTCATTAATGGATTTGCTT TGGCCTGGTTGGCAATTCGAGCAATGGCCGTGCCACGCACTGACAACATCGC TCTACCAATCTTGGCTGCTCTAACACCACTAGCTCGAGGCACACTGCTCGTG GCATGGAGAGCGGGCCTGGCTACTTGTGGAGGGATCATGCTCCTCTCCCTGAAAGGGAAAGGTAGTGTGAAGAAGAACCTGCCATTTGTCATGGCCCTGGGATT GACAGCTGTGAGGGTAGTAGACCCTATTAATGTGGTAGGACTACTGTTACTCA CAAGGAGTGGGAAGCGGAGCTGGCCCCCTAGTGAAGTTCTCACAGCCGTTG GCCTGATATGTGCACTGGCCGGAGGGTTTGCCAAGGCAGACATTGAGATGGC TGGACCCATGGCTGCAGTAGGCTTGCTAATTGTCAGCTATGTGGTCTCGGGAA AGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAGG ACGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA GTGGTGATTTCTCCTTGGTAGAGGAAGATGGTCCACCCATGAGAGAGATCAT ACTTAAGGTGGTCCTGATGGCCATCTGTGGCATGAACCCAATAGCTATACCTT TTGCTGCAGGAGCGTGGTATGTGTATGTGAAGACTGGGAAAAGGAGTGGCG CCCTCTGGGACGTGCCTGCTCCCAAAGAAGTGAAGAAAGGAGAGACCACAG ATGGAGTGTACAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAGGTTGG AGTGGGAGTCATGCAAGAGGGAGTCTTCCACACCATGTGGCACGTTACAAAAGGAGCCGCACTGAGGAGCGGTGAGGGAAGACTTGATCCATACTGGGGGGA TGTCAAGCAGGACTTGGTGTCATACTGTGGGCCTTGGAAGTTGGATGCAGCT TGGGATGGACTCAGCGAGGTACAGCTTTTGGCCGTACCTCCCGGAGAGAGG GCCAGAAACATTCAGACCCTGCCTGGAATATTCAAGACAAAGGACGGGGAC ATCGGAGCAGTTGCTCTGGACTACCCTGCAGGGACCTCAGGATCTCCGATCC TAGACAAATGTGGAAGAGTGATAGGACTCTATGGCAATGGGGTTGTGATCAA GAATGGAAGCTATGTTAGTGCTATAACCCAGGGAAAGAGGGAGGAGGAGAC TCCGGTTGAATGTTTCGAACCCTCGATGCTGAAGAAGAAGCAGCTAACTGTC TTGGATCTGCATCCAGGAGCCGGAAAAACCAGGAGAGTTCTTCCTGAAATAG TCCGTGAAGCCATAAAAAAGAGACTCCGGACAGTGATCTTGGCACCAACTA GGGTTGTCGCTGCTGAGATGGAGGAGGCCTTGAGAGGACTTCCGGTGCGTTA CATGACAACAGCAGTCAACGTCACCCATTCTGGGACAGAAATCGTTGATTTG ATGTGCCATGCCACTTTCACTTCACGCTTACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAAGCCCACTTCACAGACCCCTCAAGTATAG CTGCAAGAGGATATATATCAACAAGGGTTGAAATGGGCGAGGCGGCTGCCAT TTTTATGACTGCCACACCACCAGGAACCCGTGATGCGTTTCCTGACTCTAACT CACCAATCATGGACACAGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAG GCTTTGATTGGGTGACAGACCATTCTGGGAAAACAGTTTGGTTCGTTCCAAG CGTGAGAAACGGAAATGAAATCGCAGCCTGTCTGACAAAGGCTGGAAAGCG GGTCATACAGCTCAGCAGGAAGACTTTTGAGACAGAATTTCAGAAAACAAA AAATCAAGAGTGGGACTTTGTCATAACAACTGACATCTCAGAGATGGGCGCC AACTTCAAGGCTGACCGGGTCATAGACTCTAGGAGATGCCTAAAACCAGTCA TACTTGATGGTGAGAGAGTCATCTTGGCTGGGCCCATGCCTGTCACGCATGCT AGTGCTGCTCAGAGGAGAGGACGTATAGGCAGGAACCCTAACAAACCTGGA GATGAGTACATGTATGGAGGTGGGTGTGCAGAGACTGATGAAGGCCATGCAC ACTGGCTTGAAGCAAGAATGCTTCTTGACAACATCTACCTCCAGGATGGCCT CATAGCCTCGCTCTATCGGCCTGAGGCCGATAAGGTAGCCGCCATTGAGGGA GAGTTTAAGCTGAGGACAGAGCAAAGGAAGACCTTCGTGGAACTCATGAAG AGAGGAGACCTTCCCGTCTGGCTAGCCTATCAGGTTGCATCTGCCGGAATAA CTTACACAGACAGAAGATGGTGCTTTGATGGCACAACCAACAACACCATAAT GGAAGACAGCGTACCAGCAGAGGTGTGGACAAAGTATGGAGAGAAGAGAG TGCTCAAACCGAGATGGATGGATGCTAGGGTCTGTTCAGACCATGCGGCCCT GAAGTCGTTCAAAGAATTCGCCGCTGGAAAAAGAGGAGCGGCTTTGGGAGT AATGGAGGCCCTGGGAACACTGCCAGGACACATGACAGAGAGGTTTCAGGA AGCCATTGACAACCTCGCCGTGCTCATGCGAGCAGAGACTGGAAGCAGGCC TTATAAGGCAGCGGCAGCCCAACTGCCGGAGACCCTAGAGACCATTATGCTC TTAGGTTTGCTGGGAACAGTTTCACTGGGGATCTTCTTCGTCTTGATGCGGAA TAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCA TGGCTCATGTGGCTTTCGGAAATTGAACCAGCCAGAATTGCATGTGTCCTCAT TGTTGTGTTTTTATTACTGGTGGTGCTCATACCCGAGCCAGAGAAGCAAAGAT CTCCCCAAGATAACCAGATGGCAATTATCATCATGGTGGCAGTGGGCCTTCTA GGTTTGATAACTGCAAACGAACTTGGATGGCTGGAAAGAACAAAAAATGAC ATAGCTCATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAA TGGACATTGATCTGCGGCCAGCCTCCGCCTGGGCTATCTATGCCGCATTGACA ACTCTCATCACCCCAGCTGTCCAACATGCGGTAACCACTTCATACAACAACTA CTCCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAA GGGATGCCATTTTATGCATGGGACCTTGGAGTCCCGCTGCTAATGATGGGTTG CTATTCACAATTAACACCCCTGACTCTGATAGTAGCTATCATTCTGCTTGTGGC GCACTACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCC CAGAAAAGGACAGCAGCTGGCATCATGAAGAATCCCGTTGTGGATGGAATAG TGGTAACTGACATTGACACAATGACAATAGACCCCCAGGTGGAGAAGAAGAT GGGACAAGTGTTACTCATAGCAGTAGCCATCTCCAGTGCTGTGCTGCTGCGG ACCGCCTGGGGATGGGGGGAGGCTGGAGCTCTGATCACAGCAGCGACCTCC ACCTTGTGGGAAGGCTCTCCAAACAAATACTGGAACTCCTCTACAGCCACCT CACTGTGCAACATCTTCAGAGGAAGCTATCTGGCAGGAGCTTCCCTTATCTAT ACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTGGGACGGG AGAGACTCTGGGAGAGAAGTGGAAAGCTCGTCTGAATCAGATGTCGGCCCT GGAGTTCTACTCTTATAAAAAGTCAGGTATCACTGAAGTGTGTAGAGAGGAG GCTCGCCGTGCCCTCAAGGATGGAGTGGCCACAGGAGGACATGCCGTATCCC GGGGAAGTGCAAAGCTCAGATGGTTGGTGGAGAGAGGATATCTGCAGCCCTA TGGGAAGGTTGTTGACCTCGGATGTGGCAGAGGGGGCTGGAGCTATTATGCC GCCACCATCCGCAAAGTGCAGGAGGTGAGAGGATACACAAAGGGAGGTCCC GGTCATGAAGAACCCATGCTGGTGCAAAGCTATGGGTGGAACATAGTTCGTC TCAAGAGTGGAGTGGACGTCTTCCACATGGCGGCTGAGCCGTGTGACACTCT GCTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAGACACGA ACACTCAGAGTGCTCTCTATGGTGGGGGACTGGCTTGAAAAAAGACCAGGG GCCTTCTGTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCAT GGAGCGACTGCAACGTAGGCATGGGGGAGGATTAGTCAGAGTGCCATTGTCT CGCAACTCCACACATGAGATGTACTGGGTCTCTGGGGCAAAGAGCAACATCATAAAAAGTGTGTCCACCACAAGTCAGCTCCTCCTGGGACGCATGGATGGCCC CAGGAGGCCAGTGAAATATGAGGAGGATGTGAACCTCGGCTCGGGTACACG AGCTGTGGCAAGCTGTGCTGAGGCTCCTAACATGAAAATCATCGGCAGGCGC ATTGAGAGAATCCGCAATGAACATGCAGAAACATGGTTTCTTGATGAAAACC ACCCATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGG ATCAGCGTCTTCCCTCGTGAACGGGGTTGTTAGACTCCTGTCAAAGCCTTGG GACGTGGTGACTGGAGTTACAGGAATAGCCATGACTGACACCACACCATACG GCCAACAAAGAGTCTTCAAAGAAAAAGTGGACACCAGGGTGCCAGATCCCC AAGAAGGCACTCGCCAGGTAATGAACATAGTCTCTTCCTGGCTGTGGAAGGA GCTGGGGAAACGCAAGCGGCCACGCGTCTGCACCAAAGAAGAGTTTATCAA CAAGGTGCGCAGCAATGCAGCACTGGGAGCAATATTTGAAGAGGAAAAAGA ATGGAAGACGGCTGTGGAAGCTGTGAATGATCCAAGGTTTTGGGCCCTAGTG GATAGGGAGAGAGAACACCACCTGAGAGGAGAGTGTCACAGCTGTGTGTACAACATGATGGGAAAAAGAGAAAAGAAGCAAGGAGAGTTCGGGAAAGCAAA AGGTAGCCGCGCCATCTGGTACATGTGGTTGGGAGCCAGATTCTTGGAGTTT GAAGCCCTTGGATTCTTGAACGAGGACCATTGGATGGGAAGAGAAAACTCA GGAGGTGGAGTCGAAGGGTTAGGATTGCAAAGACTTGGATACATTCTAGAAG AAATGAATCGGGCACCAGGAGGAAAGATGTACGCAGATGACACTGCTGGCT GGGACACCCGCATTAGTAAGTTTGATCTGGAGAATGAAGCTCTGATTACCAA CCAAATGGAGGAAGGGCACAGAACTCTGGCGTTGGCCGTGATTAAATACACA TACCAAAACAAAGTGGTGAAGGTTCTCAGACCAGCTGAAGGAGGAAAAAC AGTTATGGACATCATTTCAAGACAAGACCAGAGAGGGAGTGGACAAGTTGT CACTTATGCTCTCAACACATTCACCAACTTGGTGGTGCAGCTTATCCGGAACA TGGAAGCTGAGGAAGTGTTAGAGATGCAAGACTTATGGTTGTTGAGGAAGC CAGAGAAAGTGACCAGATGGTTGCAGAGCAATGGATGGGATAGACTCAAAC GAATGGCGGTCAGTGGAGATGACTGCGTTGTGAAGCCAATCGATGATAGGTT TGCACATGCCCTCAGGTTCTTGAATGACATGGGAAAAGTTAGGAAAGACACA CAGGAGTGGAAACCCTCGACTGGATGGAGCAATTGGGAAGAAGTCCCGTTC TGCTCCCACCACTTCAACAAGCTGTACCTCAAGGATGGGAGATCCATTGTGG TCCCTTGCCGCCACCAAGATGAACTGATTGGCCGAGCTCGCGTCTCACCAGG GGCAGGATGGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCAG ATGTGGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGC CATTTGCTCGGCTGTGCCAGTTGACTGGGTACCAACTGGGAGAACCACCTGG TCAATCCATGGAAAGGGAGAATGGATGACCACTGAGGACATGCTCATGGTGT GGAATAGAGTGTGGATTGAGGAGAACGACCATATGGAGGACAAGACTCCTGT AACAAAATGGACAGACATTCCCTATCTAGGAAAAAGGGAGGACTTATGGTGT GGATCCCTTATAGGGCACAGACCCCGCACCACTTGGGCTGAAAACATCAAAG ACACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGA CTATCTATCCACCCAAGTCCGCTACTTGGGTGAGGAAGGGTCCACACCCGGAGTGTTGTAAGCACCAATTTTAGTGTTGTCAGGCCTGCTAGTCAGCCACAGTTT GGGGAAAGCTGTGCAGCCTGTAACCCCCCCAGGAGAAGCTGGGAAACCAAG CTCATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTG AGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAAGCGCAG GATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAA CTGGAGACTAGCTGTGAATCTCCAGCAGAGGGACTAGTGGTTAGAGGAGAC CCCCCGGAAAACGCAAAACAGCATATTGACGCTGGGAAAGACCAGAGACTC CATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAACAGCGGCG GCCGGTGTGGGGAAATCCATGGTTTCT
SEQ ID NO：12
AY632535.2NC_012532.1Zika病毒株MR 766，乌干达，完整的基因组
AGTTGTTGATCTGTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGA GAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTC TGGTCATGAAAAACCCCAAAGAAGAAATCCGGAGGATCCGGATTGTCAATAT GCTAAAACGCGGAGTAGCCCGTGTAAACCCCTTGGGAGGTTTGAAGAGGTT GCCAGCCGGACTTCTGCTGGGTCATGGACCCATCAGAATGGTTTTGGCGATA CTAGCCTTTTTGAGATTTACAGCAATCAAGCCATCACTGGGCCTTATCAACAG ATGGGGTTCCGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAA GAAAGATCTTGCTGCCATGTTGAGAATAATCAATGCTAGGAAAGAGAGGAAG AGACGTGGCGCAGACACCAGCATCGGAATCATTGGCCTCCTGCTGACTACAG CCATGGCAGCAGAGATCACTAGACGCGGGAGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAAC AAGTGCCACGTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGA GTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTG CTGGTGCAACACGACATCAACTTGGGTTGTGTACGGAACCTGTCATCACAAA AAAGGTGAGGCACGGCGATCTAGAAGAGCCGTGACGCTCCCTTCTCACTCTA CAAGGAAGTTGCAAACGCGGTCGCAGACCTGGTTAGAATCAAGAGAATACA CGAAGCACTTGATCAAGGTTGAAAACTGGATATTCAGGAACCCCGGGTTTGC GCTAGTGGCCGTTGCCATTGCCTGGCTTTTGGGAAGCTCGACGAGCCAAAAA GTCATATACTTGGTCATGATACTGCTGATTGCCCCGGCATACAGTATCAGGTGC ATTGGAGTCAGCAATAGAGACTTCGTGGAGGGCATGTCAGGTGGGACCTGG GTTGATGTTGTCTTGGAACATGGAGGCTGCGTTACCGTGATGGCACAGGACA AGCCAACAGTCGACATAGAGTTGGTCACGACGACGGTTAGTAACATGGCCGA GGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTTCGGACAGTC GTTGCCCAACACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATA TGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACT TTTTGGCAAAGGGAGCTTGGTGACATGTGCCAAGTTTACGTGTTCTAAGAAG ATGACCGGGAAGAGCATTCAACCGGAAAATCTGGAGTATCGGATAATGCTAT CAGTGCATGGCTCCCAGCATAGCGGGATGATTGGATATGAAACTGACGAAGA TAGAGCGAAAGTCGAGGTTACGCCTAATTCACCAAGAGCGGAAGCAACCTT GGGAGGCTTTGGAAGCTTAGGACTTGACTGTGAACCAAGGACAGGCCTTGA CTTTTCAGATCTGTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACA AAGAGTGGTTTCATGACATCCCATTGCCTTGGCATGCTGGGGCAGACACCGG AACTCCACACTGGAACAACAAAGAGGCATTGGTAGAATTCAAGGATGCCCACGCCAAGAGGCAAACCGTCGTCGTTCTGGGGAGCCAGGAAGGAGCCGTTCA CACGGCTCTCGCTGGAGCTCTAGAGGCTGAGATGGATGGTGCAAAGGGAAG GCTGTTCTCTGGCCATTTGAAATGCCGCCTAAAAATGGACAAGCTTAGATTGA AGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACATTCACCAAGGTCCCA GCTGAAACACTGCATGGAACAGTCACAGTGGAGGTGCAGTATGCAGGGACA GATGGACCCTGCAAGATCCCAGTCCAGATGGCGGTGGACATGCAGACCCTGA CCCCAGTTGGAAGGCTGATAACCGCCAACCCCGTGATTACTGAAAGCACTGA GAACTCAAAGATGATGTTGGAGCTTGACCCACCATTTGGGGATTCTTACATTG TCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGGTAG CACCATCGGAAAGGCATTTGAGGCCACTGTGAGAGGCGCCAAGAGAATGGC AGTCCTGGGGGATACAGCCTGGGACTTCGGATCAGTCGGGGGTGTGTTCAAC TCACTGGGTAAGGGCATTCACCAGATTTTTGGAGCAGCCTTCAAATCACTGTT TGGAGGAATGTCCTGGTTCTCACAGATCCTCATAGGCACGCTGCTAGTGTGG TTAGGTTTGAACACAAAGAATGGATCTATCTCCCTCACATGCTTGGCCCTGGG GGGAGTGATGATCTTCCTCTCCACGGCTGTTTCTGCTGACGTGGGGTGCTCA GTGGACTTCTCAAAAAAGGAAACGAGATGTGGCACGGGGGTATTCATCTATA ATGATGTTGAAGCCTGGAGGGACCGGTACAAGTACCATCCTGACTCCCCCCG CAGATTGGCAGCAGCAGTCAAGCAGGCCTGGGAAGAGGGGATCTGTGGGAT CTCATCCGTTTCAAGAATGGAAAACATCATGTGGAAATCAGTAGAAGGGGAG CTCAATGCTATCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGAT CTGTAAAAAACCCCATGTGGAGAGGTCCACAAAGATTGCCAGTGCCTGTGAA TGAGCTGCCCCATGGCTGGAAAGCCTGGGGGAAATCGTATTTTGTTAGGGCG GCAAAGACCAACAACAGTTTTGTTGTCGACGGTGACACACTGAAGGAATGTCCGCTTGAGCACAGAGCATGGAATAGTTTTCTTGTGGAGGATCACGGGTTTG GAGTCTTCCACACCAGTGTCTGGCTTAAGGTCAGAGAAGATTACTCATTAGA ATGTGACCCAGCCGTCATAGGAACAGCTGTTAAGGGAAGGGAGGCCGCGCA CAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACATGGAGGCTG AAGAGGGCCCACCTGATTGAGATGAAAACATGTGAATGGCCAAAGTCTCAC ACATTGTGGACAGATGGAGTAGAAGAAAGTGATCTTATCATACCCAAGTCTTT AGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAACCCAAGT GAAAGGGCCATGGCACAGTGAAGAGCTTGAAATCCGGTTTGAGGAATGTCC AGGCACCAAGGTTTACGTGGAGGAGACATGCGGAACTAGAGGACCATCTCT GAGATCAACTACTGCAAGTGGAAGGGTCATTGAGGAATGGTGCTGTAGGGAA TGCACAATGCCCCCACTATCGTTTCGAGCAAAAGACGGCTGCTGGTATGGAA TGGAGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGG TGACAGCGGGGTCAACCGATCATATGGACCACTTCTCTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAGAGAATGACCACAAAGAT CATCATGAGCACATCAATGGCAGTGCTGGTAGTCATGATCTTGGGAGGATTTT CAATGAGTGACCTGGCCAAGCTTGTGATCCTGATGGGTGCTACTTTCGCAGA AATGAACACTGGAGGAGATGTAGCTCACTTGGCATTGGTAGCGGCATTTAAA GTCAGACCAGCCTTGCTGGTCTCCTTCATTTTCAGAGCCAATTGGACACCCC GTGAGAGCATGCTGCTAGCCCTGGCTTCGTGTCTTCTGCAAACTGCGATCTCT GCTCTTGAAGGTGACTTGATGGTCCTCATTAATGGATTTGCTTTGGCCTGGTT GGCAATTCGAGCAATGGCCGTGCCACGCACTGACAACATCGCTCTACCAATC TTGGCTGCTCTAACACCACTAGCTCGAGGCACACTGCTCGTGGCATGGAGAG CGGGCCTGGCTACTTGTGGAGGGATCATGCTCCTCTCCCTGAAAGGGAAAGGTAGTGTGAAGAAGAACCTGCCATTTGTCATGGCCCTGGGATTGACAGCTGTG AGGGTAGTAGACCCTATTAATGTGGTAGGACTACTGTTACTCACAAGGAGTG GGAAGCGGAGCTGGCCCCCTAGTGAAGTTCTCACAGCCGTTGGCCTGATATG TGCACTGGCCGGAGGGTTTGCCAAGGCAGACATTGAGATGGCTGGACCCAT GGCTGCAGTAGGCTTGCTAATTGTCAGCTATGTGGTCTCGGGAAAGAGTGTG GACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAGGACGCGGAA GTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAGTGGTGAC TTCTCCTTGGTAGAGGAAGATGGTCCACCCATGAGAGAGATCATACTCAAGG TGGTCCTGATGGCCATCTGTGGCATGAACCCAATAGCTATACCTTTTGCTGCA GGAGCGTGGTATGTGTATGTGAAGACTGGGAAAAGGAGTGGCGCCCTCTGG GACGTGCCTGCTCCCAAAGAAGTGAAGAAAGGAGAGACCACAGATGGAGT GTACAGAGTGATGACTCGCAGACTGCTAGGTTCAACACAGGTTGGAGTGGG AGTCATGCAAGAGGGAGTCTTCCACACCATGTGGCACGTTACAAAAGGAGC CGCACTGAGGAGCGGTGAGGGAAGACTTGATCCATACTGGGGGGATGTCAA GCAGGACTTGGTGTCATACTGTGGGCCTTGGAAGTTGGATGCAGCTTGGGAT GGACTCAGCGAGGTACAGCTTTTGGCCGTACCTCCCGGAGAGAGGGCCAGA AACATTCAGACCCTGCCTGGAATATTCAAGACAAAGGACGGGGACATCGGA GCAGTTGCTCTGGACTACCCTGCAGGGACCTCAGGATCTCCGATCCTAGACA AATGTGGAAGAGTGATAGGACTCTATGGCAATGGGGTTGTGATCAAGAATGG AAGCTATGTTAGTGCTATAACCCAGGGAAAGAGGGAGGAGGAGACTCCGGT TGAATGTTTCGAACCCTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTGGAT CTGCATCCAGGAGCCGGAAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTG AAGCCATAAAAAAGAGACTCCGGACAGTGATCTTGGCACCAACTAGGGTTG TCGCTGCTGAGATGGAGGAGGCCTTGAGAGGACTTCCGGTGCGTTACATGAC AACAGCAGTCAACGTCACCCATTCTGGGACAGAAATCGTTGATTTGATGTGC CATGCCACTTTCACTTCACGCTTACTACAACCCATCAGAGTCCCTAATTACAA TCTCAACATCATGGATGAAGCCCACTTCACAGACCCCTCAAGTATAGCTGCA AGAGGATACATATCAACAAGGGTTGAAATGGGCGAGGCGGCTGCCATTTTTA TGACTGCCACACCACCAGGAACCCGTGATGCGTTTCCTGACTCTAACTCACC AATCATGGACACAGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTT TGATTGGGTGACAGACCATTCTGGGAAAACAGTTTGGTTCGTTCCAAGCGTG AGAAACGGAAATGAAATCGCAGCCTGTCTGACAAAGGCTGGAAAGCGGGTC ATACAGCTCAGCAGGAAGACTTTTGAGACAGAATTTCAGAAAACAAAAAAT CAAGAGTGGGACTTTGTCATAACAACTGACATCTCAGAGATGGGCGCCAACT TCAAGGCTGACCGGGTCATAGACTCTAGGAGATGCCTAAAACCAGTCATACT TGATGGTGAGAGAGTCATCTTGGCTGGGCCCATGCCTGTCACGCATGCTAGT GCTGCTCAGAGGAGAGGACGTATAGGCAGGAACCCTAACAAACCTGGAGAT GAGTACATGTATGGAGGTGGGTGTGCAGAGACTGATGAAGGCCATGCACACT GGCTTGAAGCAAGAATGCTTCTTGACAACATCTACCTCCAGGATGGCCTCATAGCCTCGCTCTATCGGCCTGAGGCCGATAAGGTAGCCGCCATTGAGGGAGAG TTTAAGCTGAGGACAGAGCAAAGGAAGACCTTCGTGGAACTCATGAAGAGA GGAGACCTTCCCGTCTGGCTAGCCTATCAGGTTGCATCTGCCGGAATAACTTA CACAGACAGAAGATGGTGCTTTGATGGCACAACCAACAACACCATAATGGA AGACAGTGTACCAGCAGAGGTTTGGACAAAGTATGGAGAGAAGAGAGTGCT CAAACCGAGATGGATGGATGCTAGGGTCTGTTCAGACCATGCGGCCCTGAAG TCGTTCAAAGAATTCGCCGCTGGAAAAAGAGGAGCGGCTTTGGGAGTAATG GAGGCCCTGGGAACACTGCCAGGACACATGACAGAGAGGTTTCAGGAAGCC ATTGACAACCTCGCCGTGCTCATGCGAGCAGAGACTGGAAGCAGGCCTTATA AGGCAGCGGCAGCCCAACTGCCGGAGACCCTAGAGACCATTATGCTCTTAGG TTTGCTGGGAACAGTTTCACTGGGGATCTTCTTCGTCTTGATGCGGAATAAGG GCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCT CATGTGGCTTTCGGAAATTGAACCAGCCAGAATTGCATGTGTCCTCATTGTTG TGTTTTTATTACTGGTGGTGCTCATACCCGAGCCAGAGAAGCAAAGATCTCCC CAAGATAACCAGATGGCAATTATCATCATGGTGGCAGTGGGCCTTCTAGGTTT GATAACTGCAAACGAACTTGGATGGCTGGAAAGAACAAAAAATGACATAGC TCATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGA CATTGATCTGCGGCCAGCCTCCGCCTGGGCTATCTATGCCGCATTGACAACTC TCATCACCCCAGCTGTCCAACATGCGGTAACCACTTCATACAACAACTACTCC TTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAAGGGA TGCCATTTATGCATGGGGACCTTGGAGTCCCGCTGCTAATGATGGGTTGCTAT TCACAATTAACACCCCTGACTCTGATAGTAGCTATCATTCTGCTTGTGGCGCA CTACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG AAAAGGACAGCAGCTGGCATCATGAAGAATCCCGTTGTGGATGGAATAGTGG TAACTGACATTGACACAATGACAATAGACCCCCAGGTGGAGAAGAAGATGG GACAAGTGTTACTCATAGCAGTAGCCATCTCCAGTGCTGTGCTGCTGCGGAC CGCCTGGGGATGGGGGGAGGCTGGAGCTCTGATCACAGCAGCGACCTCCAC CTTGTGGGAAGGCTCTCCAAACAAATACTGGAACTCCTCTACAGCCACCTCA CTGTGCAACATCTTCAGAGGAAGCTATCTGGCAGGAGCTTCCCTTATCTATAC AGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTGGGACGGGAG AGACTCTGGGAGAGAAGTGGAAAGCTCGTCTGAATCAGATGTCGGCCCTGG AGTTCTACTCTTATAAAAAGTCAGGTATCACTGAAGTGTGTAGAGAGGAGGC TCGCCGTGCCCTCAAGGATGGAGTGGCCACAGGAGGACATGCCGTATCCCGG GGAAGTGCAAAGATCAGATGGTTGGAGGAGAGAGGATATCTGCAGCCCTATG GGAAGGTTGTTGACCTCGGATGTGGCAGAGGGGGCTGGAGCTATTATGCCGC CACCATCCGCAAAGTGCAGGAGGTGAGAGGATACACAAAGGGAGGTCCCGG TCATGAAGAACCCATGCTGGTGCAAAGCTATGGGTGGAACATAGTTCGTCTC AAGAGTGGAGTGGACGTCTTCCACATGGCGGCTGAGCCGTGTGACACTCTG CTGTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAGACACGAA CACTCAGAGTGCTCTCTATGGTGGGGGACTGGCTTGAAAAAAGACCAGGGG CCTTCTGTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATG GAGCGACTGCAACGTAGGCATGGGGGAGGATTAGTCAGAGTGCCATTGTGTC GCAACTCCACACATGAGATGTACTGGGTCTCTGGGGCAAAGAGCAACATCAT AAAAAGTGTGTCCACCACAAGTCAGCTCCTCCTGGGACGCATGGATGGCCCC AGGAGGCCAGTGAAATATGAGGAGGATGTGAACCTCGGCTCGGGTACACGA GCTGTGGCAAGCTGTGCTGAGGCTCCTAACATGAAAATCATCGGCAGGCGCATTGAGAGAATCCGCAATGAACATGCAGAAACATGGTTTCTTGATGAAAACCA CCCATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGGA TCAGCGTCTTCCCTCGTGAACGGGGTTGTTAGACTCCTGTCAAAGCCTTGGG ACGTGGTGACTGGAGTTACAGGAATAGCCATGACTGACACCACACCATACGG CCAACAAAGAGTCTTCAAAGAAAAAGTGGACACCAGGGTGCCAGATCCCCA AGAAGGCACTCGCCAGGTAATGAACATAGTCTCTTCCTGGCTGTGGAAGGAG CTGGGGAAACGCAAGCGGCCACGCGTCTGCACCAAAGAAGAGTTTATCAAC AAGGTGCGCAGCAATGCAGCACTGGGAGCAATATTTGAAGAGGAAAAAGAA TGGAAGACGGCTGTGGAAGCTGTGAATGATCCAAGGTTTTGGGCCCTAGTGG ATAGGGAGAGAGAACACCACCTGAGAGGAGAGTGTCACAGCTGTGTGTACA ACATGATGGGAAAAAGAGAAAAGAAGCAAGGAGAGTTCGGGAAAGCAAAA GGTAGCCGCGCCATCTGGTACATGTGGTTGGGAGCCAGATTCTTGGAGTTTG AAGCCCTTGGATTCTTGAACGAGGACCATTGGATGGGAAGAGAAAACTCAG GAGGTGGAGTCGAAGGGTTAGGATTGCAAAGACTTGGATACATTCTAGAAGA AATGAATCGGGCACCAGGAGGAAAGATGTACGCAGATGACACTGCTGGCTG GGACACCCGCATTAGTAAGTTTGATCTGGAGAATGAAGCTCTGATTACCAAC CAAATGGAGGAAGGGCACAGAACTCTGGCGTTGGCCGTGATTAAATACACAT ACCAAAACAAAGTGGTGAAGGTTCTCAGACCAGCTGAAGGAGGAAAAACA GTTATGGACATCATTTCAAGACAAGACCAGAGAGGGAGTGGACAAGTTGTC ACTTATGCTCTCAACACATTCACCAACTTGGTGGTGCAGCTTATCCGGAACAT GGAAGCTGAGGAAGTGTTAGAGATGCAAGACTTATGGTTGTTGAGGAAGCC AGAGAAAGTGACCAGATGGTTGCAGAGCAATGGATGGGATAGACTCAAACG AATGGCGGTCAGTGGAGATGACTGCGTTGTGAAGCCAATCGATGATAGGTTT GCACATGCCCTCAGGTTCTTGAATGACATGGGAAAAGTTAGGAAAGACACAC AGGAGTGGAAACCCTCGACTGGATGGAGCAATTGGGAAGAAGTCCCGTTCT GCTCCCACCACTTCAACAAGCTGTACCTCAAGGATGGGAGATCCATTGTGGT CCCTTGCCGCCACCAAGATGAACTGATTGGCCGAGCTCGCGTCTCACCAGGG GCAGGATGGAGCATCCGGGAGACTGCCTGTCTTGCAAAATCATATGCGCAGA TGTGGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC ATTTGCTCGGCTGTGCCAGTTGACTGGGTACCAACTGGGAGAACCACCTGGT CAATCCATGGAAAGGGAGAATGGATGACCACTGAGGACATGCTCATGGTGTG GAATAGAGTGTGGATTGAGGAGAACGACCATATGGAGGACAAGACTCCTGTA ACAAAATGGACAGACATTCCCTATCTAGGAAAAAGGGAGGACTTATGGTGTG GATCCCTTATAGGGCACAGACCCCGCACCACTTGGGCTGAAAACATCAAAGA CACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGAC TATCTATCCACCCAAGTCCGCTACTTGGGTGAGGAAGGGTCCACACCCGGAG TGTTGTAAGCACCAATTTTAGTGTTGTCAGGCCTGCTAGTCAGCCACAGTTTG GGGAAAGCTGTGCAGCCTGTAACCCCCCCAGGAGAAGCTGGGAAACCAAGC TCATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAAGCGCAG GATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAA CTGGAGACTAGCTGTGAATCTCCAGCAGAGGGACTAGTGGTTAGAGGAGAC CCCCCGGAAAACGCAAAACAGCATATTGACGTGGGAAAGACCAGAGACTCC ATGAGTTTCCACCACGCTGGCCGCCAGGCACAGATCGCCGAACTTCGGCGGC CGGTGTGGGGAAATCCATGGTTTCT
SEQ ID NO：13
KJ776791.1，Zika病毒株H/PF/2013多聚蛋白基因，完整的cds
AGTATCAACAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATG AAAAACCCAAAAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAAC GCGGAGTAGCCCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCG GACTTCTGCTGGGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTT TTGAGATTCACGGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTC AGTGGGGAAAAAAGAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCT GGCTGCCATGCTGAGAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGG CGCAGATACTAGTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCA GCGGAGGTCACTAGACGTGGGAGTGCATACTATATGTACTTGGACAGAAACG ACGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGTTAT ATACAGATCATGGATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATG CCCTATGCTGGATGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAAC ACGACGTCAACTTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAA GCACGGAGATCTAGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGC TGCAAACGCGGTCGCAAACCTGGTTGGAATCAAGAGAATACACAAAGCACT TGATTAGAGTCGAAAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGC AGCTGCCATCGCTTGGCTTTTGGGAAGCTCAACGAGCCAAAAAGTCATATAC TTGGTCATGATACTGCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGT CAGCAATAGGGACTTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTT GTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAACCGACTGTCGACATAGAGCTGGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATC CTACTGCTATGAGGCATCAATATCGGACATGGCTTCGGACAGCCGCTGCCCAA CACAAGGTGAAGCCTACCTTGACAAGCAATCAGACACTCAATATGTCTGCAA AAGAACGTTAGTGGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAA AGGGAGCCTGGTGACATGCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGG AAGAGCATCCAGCCAGAGAATCTGGAGTACCGGATAATGCTGTCAGTTCATG GCTCCCAGCACAGTGGGATGATCGTTAATGACACAGGACATGAAACTGATGA GAATAGAGCGAAGGTTGAGATAACGCCCAATTCACCAAGAGCCGAAGCCAC CCTGGGGGGTTTTGGAAGCCTAGGACTTGATTGTGAACCGAGGACAGGCCTT GACTTTTCAGATTTGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCA CAAGGAGTGGTTCCACGACATTCCATTACCTTGGCACGCTGGGGCAGACACC GGAACTCCACACTGGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCA CATGCCAAAAGGCAAACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTT CACACGGCCCTTGCTGGAGCTCTGGAGGCTGAGATGGATGGTGCAAAGGGA AGGCTGTCCTCTGGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATT GAAGGGCGTGTCATACTCCTTGTGTACCGCAGCGTTCACATTCACCAAGATC CCGGCTGAAACACTGCACGGGACAGTCACAGTGGAGGTACAGTACGCAGGG ACAGATGGACCTTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTC TGACCCCAGTTGGGAGGTTGATAACCGCTAACCCCGTAATCACTGAAAGCAC TGAGAACTCTAAGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACA TTGTCATAGGAGTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTG GCAGCACCATTGGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAA TGGCAGTCTTGGGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCT CAACTCATTGGGCAAGGGCATCCATCAAATTTTTGGAGCAGCTTTCAAATCAT TGTTTGGAGGAATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTGCTGATG TGGTTGGGTCTGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTT AGGGGGAGTGTTGATCTTCTTATCCACAGCTGTCTCTGCTGATGTGGGGTGCT CGGTGGACTTCTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCT ATAACGACGTTGAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCC CCGTAGATTGGCAGCAGCAGTCAAGCAAGCCTGGGAAGATGGTATCTGTGGG ATCTCCTCTGTTTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGG AGCTCAACGCAATCCTGGAAGAGAATGGAGTTCAACTGACGGTCGTTGTGG GATCTGTAAAAAACCCCATGTGGAGAGGTCCACAGAGATTGCCCGTGCCTGT GAACGAGCTGCCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAG AGCAGCAAAGACAAATAACAGCTTTGTCGTGGATGGTGACACACTGAAGGA ATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGG TTCGGGGTATTTCACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATT AGAGTGTGATCCAGCCGTTATTGGAACAGCTGTTAAGGGAAAGGAGGCTGTA CACAGTGATCTAGGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGC TGAAGAGGGCCCATCTGATCGAGATGAAAACATGTGAATGGCCAAAGTCCCA CACATTGTGGACAGATGGAATAGAAGAGAGTGATCTGATCATACCCAAGTCT TTAGCTGGGCCACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAA TGAAAGGGCCATGGCACAGTGAAGAGCTTGAAATTCGGTTTGAGGAATGCC CAGGCACTAAGGTCCACGTGGAGGAAACATGTGGAACAAGAGGACCATCTC TGAGATCAACCACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGG AGTGCACAATGCCCCCACTGTCGTTCCGGGCTAAAGATGGCTGTTGGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAAAGTAACTTAGTAAGGTCAAT GGTGACTGCAGGATCAACTGATCACATGGATCACTTCTCCCTTGGAGTGCTT GTGATTCTGCTCATGGTGCAGGAAGGGCTGAAGAAGAGAATGACCACAAAG ATCATCATAAGCACATCGATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATT TTCAATGAGTGACCTGGCTAAGCTTGCAATTTTGATGGGTGCCACCTTCGCGG AAATGAACACTGGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGCATTCAA AGTCAGACCAGCGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCC GTGAAAGCATGCTGCTGGCCTTGGCCTCGTGTCTTTTGCAAACTGCGATCTC CGCCTTGGAAGGCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGT TGGCAATACGAGCGATGGTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGCTCTGACACCACTGGCCCGGGGCACACTGCTTGTGGCGTGGAGA GCAGGCCTTGCTACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAG GCAGTGTGAAGAAGAACTTACCATTTGTCATGGCCCTGGGACTAACCGCTGT GAGGCTGGTCGACCCCATCAACGTGGTGGGACTGCTGTTGCTCACAAGGAG TGGGAAGCGGAGCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATA TGCGCATTGGCTGGAGGGTTCGCCAAGGCAGATATAGAGATGGCTGGGCCCA TGGCCGCGGTCGGTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGT GGACATGTACATTGAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGA AGTCACTGGAAACAGTCCCCGGCTCGATGTGGCGCTAGATGAGAGTGGTGAT TTCTCCCTGGTGGAGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGG TGGTCCTGATGACCATCTGTGGCATGAACCCAATAGCCATACCCTTTGCAGCT GGAGCGTGGTACGTATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGG GATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGACTCGTAGACTGCTAGGTTCAACACAAGTTGGAGTGGGAG TTATGCAAGAGGGGGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCGC GCTGAGAAGCGGTGAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCA GGATCTGGTGTCATACTGTGGTCCATGGAAGCTAGATGCCGCCTGGGACGGG CACAGCGAGGTGCAGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAAC ATCCAGACTCTGCCCGGAATATTTAAGACAAAGGATGGGGACATTGGAGCGG TTGCGCTGGATTACCCAGCAGGAACTTCAGGATCTCCAATCCTAGACAAGTG TGGGAGAGTGATAGGACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGT TATGTTAGTGCCATCACCCAAGGGAGGAGGGAGGAAGAGACTCCTGTTGAG TGCTTCGAGCCTTCGATGCTGAAGAAGAAGCAGCTAACTGTCTTAGACTTGC ATCCTGGAGCTGGGAAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGC CATAAAAACAAGACTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCT GCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGTCAATGTCACCCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGC CACCTTCACTTCACGTCTACTACAGCCAATCAGAGTCCCCAACTATAATCTGT ATATTATGGATGAGGCCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGA TACATTTCAACAAGGGTTGAGATGGGCGAGGCGGCTGCCATCTTCATGACCG CCACGCCACCAGGAACCCGTGACGCATTTCCGGACTCCAACTCACCAATTAT GGACACCGAAGTGGAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTG GGTGACGGATCATTCTGGAAAAACAGTTTGGTTTGTTCCAAGCGTGAGGAAC GGCAATGAGATCGCAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAG CTCAGCAGAAAGACTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAG TGGGACTTTGTCGTGACAACTGACATTTCAGAGATGGGCGCCAACTTTAAAG CTGACCGTGTCATAGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGC GAGAGAGTCATTCTGGCTGGACCCATGCCTGTCACACATGCCAGCGCTGCCC AGAGGAGGGGGCGCATAGGCAGGAATCCCAACAAACCTGGAGATGAGTATC TGTATGGAGGTGGGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTG AAGCAAGAATGCTCCTTGACAATATTTACCTCCAAGATGGCCTCATAGCCTCG CTCTATCGACCTGAGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAG CTTAGGACGGAGCAAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGAT CTTCCTGTTTGGCTGGCCTATCAGGTTGCATCTGCCGGAATAACCTACACAGA TAGAAGATGGTGCTTTGATGGCACGACCAACAACACCATAATGGAAGACAGT GTGCCGGCAGAGGTGTGGACCAGACACGGAGAGAAAAGAGTGCTCAAACC GAGGTGGATGGACGCCAGAGTTTGTTCAGATCATGCGGCCCTGAAGTCATTC AAGGAGTTTGCCGCTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCC CTGGGAACACTGCCAGGACACATGACAGAGAGATTCCAGGAAGCCATTGAC AACCTCGCTGTGCTCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCC GCGGCGGCCCAATTGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGC TGGGAACAGTCTCGCTGGGAATCTTTTTCGTCTTGATGAGGAACAAGGGCAT AGGGAAGATGGGCTTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATG TGGCTCTCGGAAATTGAGCCAGCCAGAATTGCATGTGTCCTCATTGTTGTGTT CCTATTGCTGGTGGTGCTCATACCTGAGCCAGAAAAGCAAAGATCTCCCCAG GACAACCAAATGGCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGAT TACCGCCAATGAACTCGGATGGTTGGAGAGAACAAAGAGTGACCTAAGCCA TCTAATGGGAAGGAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACATT GACCTGCGGCCAGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCAT TACCCCAGCCGTCCAACATGCAGTGACCACTTCATACAACAACTACTCCTTAA TGGCGATGGCCACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCC ATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCAC AATTAACACCCCTGACCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTAC ATGTACTTGATCCCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGA GAACGGCAGCTGGCATCATGAAGAACCCTGTTGTGGATGGAATAGTGGTGAC TGACATTGACACAATGACAATTGACCCCCAAGTGGAGAAAAAGATGGGACA GGTGCTACTCATAGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCC TGGGGGTGGGGGGAGGCTGGGGCCCTGATCACAGCGGCAACTTCCACTTTG TGGGAAGGCTCTCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGT GTAACATTTTTAGGGGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTA ACAAGAAACGCTGGCTTGGTCAAGAGACGTGGGGGTGGAACAGGAGAGAC CCTGGGAGAGAAATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAAAAAGTCAGGCATCACCGAGGTGTGCAGAGAAGAGGCCCG CCGCGCCCTCAAGGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCGAGG AAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGG AAAGGTCATTGATCTTGGATGTGGCAGAGGGGGCTGGAGTTACTACGCCGCC ACCATCCGCAAAGTTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGT CATGAAGAACCCATGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTA AGAGTGGGGTGGACGTCTTTCATATGGCGGCTGAGCCGTGTGACACGTTGCT GTGTGACATAGGTGAGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACG CTCAGAGTCCTCTCCATGGTGGGGGATTGGCTTGAAAAAAGACCAGGAGCC TTTTGTATAAAAGTGTTGTGCCCATACACCAGCACTATGATGGAAACCCTGGA GCGACTGCAGCGTAGGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCG CAACTCTACACATGAGATGTACTGGGTCTCTGGAGCGAAAAGCAACACCATA AAAAGTGTGTCCACCACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCC AGGAGGCCAGTGAAATATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGG GCTGTGGTAAGCTGCGCTGAAGCTCCCAACATGAAGATCATTGGTAACCGCA TTGAAAGGATCCGCAGTGAGCACGCGGAAACGTGGTTCTTTGACGAGAACC ACCCATATAGGACATGGGCTTACCATGGAAGCTATGAGGCCCCCACACAAGG GTCAGCGTCCTCTCTAATAAACGGGGTTGTCAGGCTCCTGTCAAAACCCTGG GATGTGGTGACTGGAGTCACAGGAATAGCCATGACCGACACCACACCGTATG GTCAGCAAAGAGTTTTCAAGGAAAAAGTGGACACTAGGGTGCCAGACCCCC AAGAAGGCACTCGTCAGGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGA GCTAGGCAAACACAAACGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAA CAAGGTTCGTAGCAATGCAGCATTAGGGGCAATATTTGAAGAGGAAAAAGAG TGGAAGACTGCAGTGGAAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTG GACAAGGAAAGAGAGCACCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTA CAACATGATGGGAAAAAGAGAAAAGAAACAAGGGGAATTTGGAAAGGCCA AGGGCAGCCGCGCCATCTGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTC GAAGCCCTTGGATTCTTGAACGAGGATCACTGGATGGGGAGAGAGAACTCA GGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAGAGATGAGTCGCATACCAGGAGGAAGGATGTATGCAGATGACACTGCTGGCTG GGACACCCGCATCAGCAGGTTTGATCTGGAGAATGAAGCTCTAATCACCAAC CAAATGGAGAAAGGGCACAGGGCCTTGGCATTGGCCATAATCAAGTACACAT ACCAAAACAAAGTGGTAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGACA GTTATGGACATTATTTCGAGACAAGACCAAAGGGGGAGCGGACAAGTTGTCA CTTACGCTCTTAACACATTTACCAACCTAGTGGTGCAACTCATTCGGAATATG GAGGCTGAGGAAGTTCTAGAGATGCAAGACTTGTGGCTGCTGCGGAGGTCA GAGAAAGTGACCAACTGGTTGCAGAGCAACGGATGGGATAGGCTCAAACGA ATGGCAGTCAGTGGAGATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGC ACATGCCCTCAGGTTCTTGAATGATATGGGAAAAGTTAGGAAGGACACACAA GAGTGGAAACCCTCAACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGC TCCCACCACTTCAACAAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTC CCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCAGGGGCGGGATGGAGCATCCGGGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATG TGGCAGCTCCTTTATTTCCACAGAAGGGACCTCCGACTGATGGCCAATGCCAT TTGTTCATCTGTGCCAGTTGACTGGGTTCCAACTGGGAGAACTACCTGGTCA ATCCATGGAAAGGGAGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGA ACAGAGTGTGGATTGAGGAGAACGACCACATGGAAGACAAGACCCCAGTTA CGAAATGGACAGACATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGG ATCTCTCATAGGGCACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAAC ACAGTCAACATGGTGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGACT ACCTATCCACCCAAGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGT GCTGTAAGCACCAATCTTAGTGTTGTCAGGCCTGCTAGTCAGCCACAGCTTG GGGAAAGCTGTGCAGCCTGTGACCCCCCCAGGAGAAGCTGGGAAACCAAG CCTATAGTCAGGCCGAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTG AGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGAAGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAA CTGGAGATCAGCTGTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAG
在一些实施方式中，Zika病毒具有对应于由SEQ ID NO：2-13或72中的任一 个核酸序列提供的DNA序列的RNA基因组。在一些实施方式中，Zika病毒具有 与SEQ ID NO：2-13或72中的任一个具有至少80％、81％、82％、83％、84％、 85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、 98％、99％、99.5％、99.6％、99.7％、99.8％或99.9％同一性的变异基因组。
下文提供了可用于本文所述的方法、组合物和/或疫苗中的Zika菌株的E蛋白 的氨基酸序列。
SEQ ID NO：14
isol-ARB15076.AHF49784.1.Central_African_Republic/291-788黄病毒包膜糖蛋 白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDE NRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHK EWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHT ALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPA ETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGD TAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTK NGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：15
isol-IbH_30656.AEN75265.1.尼日利亚/291-788黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDE NRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHK EWFHDIPLPWHSGADTETPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHT ALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPA ETLHGTVTVEVQYAGRDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSIIGKAFEATVRGAKRMAVLGDT AWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTKN GSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：16
ArB1362.AHL43500.1.-/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND XXXXXXXNRAEVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNN KHWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLG SQEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAA FTFTKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAK RMAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLV WLGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：17
ArD128000.AHL43502.1.-/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMXXXX XGHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNN KHRLVRKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWLKKGSSIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGVHQIFGAAFKSLFGGMSWFSQILIGTLLV WLGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：18
ArD158095.AHL43505.1.-/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDI GHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：19
ArD158084.AHL43504.1.-/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDI GHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：20
isol-ARB13565.AHF49783.1.Central_African_Republic/291-794黄病毒包膜糖蛋 白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDI GHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGVHQIFGAAFKSLFGGMSWFSQILIGTLLV WLGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：21
isol-ARB7701.AHF49785.1.Central_African_Republic/291-794黄病毒包膜糖蛋 白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDI GHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGVHQIFGAAFKSLFGGMSWFSQILIGTLLV WLGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：22
isol-ArD_41519.AEN75266.1.赛内加尔/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDT GHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：23
MR766-NIID.BAP47441.1.乌干达/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMTVNDI GYETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGKLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKIPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：24
Zika病毒基因组RNA，菌株：MR766-NIID，乌干达，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMTVNDI GYETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGKLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKIPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：25
isol-MR_766.AEN75263.1.乌干达/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDT GYETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGKLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQYAGTDGPCKIPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：26
ArD7117.AHL43501.1.-/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDI GHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAVC TAAKVPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：27
AY632535.2/326-825NC_012532.1Zika病毒株MR 766，乌干达，黄病毒包膜 糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIGYET DEDRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLV HKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGA VHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTK VPAETLHGTVTVEVQYAGTDGPCKIPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKRMAV LGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGL NTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：28
MR_766.AAV34151.1.乌干达/291-790黄病毒包膜糖蛋白E.|Q32ZE1| Q32ZE1_9FL
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIGYET DEDRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLV HKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGA VHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTK VPAETLHGTVTVEVQYAGTDGPCKIPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKRMAV LGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGL NTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：29
MR_766.YP_009227198.1.乌干达/1-500包膜蛋白E[Zika病毒]
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIGYET DEDRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLV HKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGA VHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTK VPAETLHGTVTVEVQYAGTDGPCKIPVQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKRMAV LGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGL NTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：30
KU681081.3/308-811Zika病毒分离株Zika病毒/H.sapiens-tc/THA/2014/SV0127-14，泰国，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHTGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITEGTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVLNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLM WLGLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：31
isol-Zika_病毒％H.sapiens-tc％THA％2014％SV0127-_14.AMD61710.1.泰国/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHTGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITEGTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVLNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLM WLGLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：32
CK-ISL_2014.AIC06934.1.Cook_Islands/1-504黄病毒包膜糖蛋白E.(片段) OS＝Zika病毒GN＝E PE＝4SV＝1
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：33
Natal_RGN.AMB18850.1.巴西：_Rio_Grande_do_Norte，_Natal/291-794黄病 毒包膜糖蛋白E.]
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：34
isol-Si323.AMC37200.1.哥伦比亚/1-504黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：35
KU707826.1/317-820Zika病毒分离株SSABR1，巴西，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：36
KU509998.1/326-829Zika病毒株海地/1225/2014，海地，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：37
isol-GDZ16001.AML82110.1.中国/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：38
BeH819015.AMA12085.1.巴西/291-794黄病毒包膜糖蛋白E.]
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：39
MRS_OPY_Martinique_PaRi_2015.AMC33116.1.Martinique/291-794黄病毒包 膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：40
KU501215.1/308-811Zika病毒株PRVABC59，波多黎各，黄病毒包膜糖蛋白 E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：41
海地％1225％2014.AMB37295.1.海地/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：42
KU527068.1/308-811Zika病毒株Natal RGN，巴西：Rio Grande do Norte，Natal，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：43
isol-Z1106027.ALX35662.1.苏里南/5-508黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：44
isol-FLR.AMM39804.1.哥伦比亚：_Barranquilla/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：45
PLCal_ZV_isol-From_Vero_E6_cells.AHL37808.1.加拿大/254-757黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：46
BeH818995.AMA12084.1.巴西/291-794黄病毒包膜糖蛋白E.[Zika病毒]。
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：47
H/PF/2013.AHZ13508.1.法属_波利尼西亚/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：48
PRVABC59.AMC13911.1.波多黎各/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：49
KU321639.1/326-829Zika病毒株ZikaSPH2015，巴西，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDIVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：50
ZikaSPH2015.ALU33341.1.巴西/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDIVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：51
103344.AMC13912.1.瓜地马拉/291-794多蛋白[Zika病毒]。103344.AMC13912.1.危地马拉黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEIRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGN GCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDT GHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKH WLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQ EGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFT FTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITES TENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRMA VLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：52
isol-Brazil-ZKV2015.AMD16557.1.巴西/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGT QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：53
KU497555.1/308-811Zika病毒分离株巴西-ZKV2015，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGT QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：54
isol-ZJ03.AMM39806.1.中国/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGARRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：55
isol-FSS13025.AFD30972.1.柬埔寨/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：56
isol-Z1106032.ALX35660.1.苏里南/291-794黄病毒包膜糖蛋白E.[Zika病毒]
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNAKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：57
isol-Z1106033.ALX35659.1.苏里南/291-794黄病毒包膜糖蛋白E.[Zika病毒]
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNAKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：58
isol-BeH828305.AMK49165.1.巴西/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDTQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：59
isol-GD01.AMK79468.1.中国/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNG TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWL GLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：60
isol-Z1106031.ALX35661.1.苏里南/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVLAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLM WLGLNTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：61
ACD75819.1.密克罗尼西亚/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPAVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWL GLNTKNGSISLTCLALGGVLIFLSTAVSA
SEQ ID NO：62
KU681082.3/308-811Zika病毒分离株Zika病毒/H.sapiens-tc/PHL/2012/ CPC-0740，菲律宾，黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWL GLNTKNGSISLTCLALGGVLIFLSTAVSA
SEQ ID NO：63
isol-Zika_病毒％H.sapiens-tc％PHL％2012％CPC-0740.AMD61711.1.菲律宾 /291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWL GLNTKNGSISLTCLALGGVLIFLSTAVSA
SEQ ID NO：64
isol-BeH823339.AMK49164.2.巴西/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVSTTV SNMAEVRSYCYEATISDIASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWGN GCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDT GHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKH WLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTAVVLGSQE GAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAFTF TKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITEST ENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKRMAV LGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLMWLGL NTKNGSISLMCLALGGVLIFLSTAVSA
SEQ ID NO：65
isol-P6-740.AEN75264.1.马来西亚/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVND XGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWXRSGSTIGKAFEATVRGAKRM AVLGDTAWDFGSVGGALNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWL GLNTKNGSISLTCLALGGVLIFLSTAVSA
SEQ ID NO：66
KU744693.1/326-829Zika病毒分离株VE_Ganxian，中国，黄病毒包膜糖蛋白 E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTAMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMLVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLAHKEWFHDIPLPWHAGAATGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETVDGTVTVEGQYGGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGALNSLGKGIHQIIGAAFKSLFGGMSWFSQILIGTLLMW LGLNTKNGSISLMCLALGGVLIFLSTAVSG
SEQ ID NO：67
isol-VE_Ganxian.AMK79469.1.中国/291-794黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTAMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFACSKKMTGKSIQPENLEYRIMLSVHGSQHSGMLVND TGHETDENRAKVEITPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLAHKEWFHDIPLPWHAGAATGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLSSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKIPAETVDGTVTVEGQYGGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGALNSLGKGIHQIIGAAFKSLFGGMSWFSQILIGTLLMW LGLNTKNGSISLMCLALGGVLIFLSTAVSG
SEQ ID NO：68
ArD157995.AHL43503.1.-/291-794黄病毒包膜糖蛋白E.
ISCIGVSNRDLVEGMSGGTWVDVVLEHGGCVTEMAQDKPTVDIELVTMTV SNMAEVRSYCYEASLSDMASASRCPTQGEPSLDKQSDTQSVCKRTLGDRGWG NGCGIFGKGSLVTCSKFTCCKKMPGKSIQPENLEYRIMLPVHGSQHSGMIVNDI GHETDENRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNK HWLVHKEWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGS QEGAVHTALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAF TFTKVPAETLHGTVTVEVQSAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKR MAVLGDTAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVW LGLNTKNGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：69
MR_766.ABI54475.1.乌干达/291-788黄病毒包膜糖蛋白E.
IRCIGVSNRDFVEGMSGGTWVDVVLEHGGCVTVMAQDKPTVDIELVTTTV SNMAEVRSYCYEASISDMASDSRCPTQGEAYLDKQSDTQYVCKRTLVDRGWG NGCGLFGKGSLVTCAKFTCSKKMTGKSIQPENLEYRIMLSVHGSQHSGMIVNDE NRAKVEVTPNSPRAEATLGGFGSLGLDCEPRTGLDFSDLYYLTMNNKHWLVHK EWFHDIPLPWHAGADTGTPHWNNKEALVEFKDAHAKRQTVVVLGSQEGAVHT ALAGALEAEMDGAKGRLFSGHLKCRLKMDKLRLKGVSYSLCTAAFTFTKVPA ETLHGTVTVEVQYAGTDGPCKVPAQMAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGDKKITHHWHRSGSTIGKAFEATVRGAKRMAVLGD TAWDFGSVGGVFNSLGKGIHQIFGAAFKSLFGGMSWFSQILIGTLLVWLGLNTK NGSISLTCLALGGVMIFLSTAVSA
SEQ ID NO：70
5’-(dIdC)13-3’
dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC dIdC
SEQ ID NO:71
KLK肽
KLKLLLLLKLK
SEQ ID NO：72
测序的ZIKV序列H/PF/2013
CAGACTGCGACAGTTCGAGTTTGAAGCGAAAGCTAGCAACAGTATCAA CAGGTTTTATTTTGGATTTGGAAACGAGAGTTTCTGGTCATGAAAAACCCAA AAAAGAAATCCGGAGGATTCCGGATTGTCAATATGCTAAAACGCGGAGTAGC CCGTGTGAGCCCCTTTGGGGGCTTGAAGAGGCTGCCAGCCGGACTTCTGCTG GGTCATGGGCCCATCAGGATGGTCTTGGCGATTCTAGCCTTTTTGAGATTCAC GGCAATCAAGCCATCACTGGGTCTCATCAATAGATGGGGTTCAGTGGGGAAA AAAGAGGCTATGGAAATAATAAAGAAGTTCAAGAAAGATCTGGCTGCCATGC TGAGAATAATCAATGCTAGGAAGGAGAAGAAGAGACGAGGCGCAGATACTA GTGTCGGAATTGTTGGCCTCCTGCTGACCACAGCTATGGCAGCGGAGGTCAC TAGACGTGGGAGTGCATACTATATGTACTTGGACAGAAACGACGCTGGGGAGGCCATATCTTTTCCAACCACATTGGGGATGAATAAGTGTTATATACAGATCATG GATCTTGGACACATGTGTGATGCCACCATGAGCTATGAATGCCCTATGCTGGA TGAGGGGGTGGAACCAGATGACGTCGATTGTTGGTGCAACACGACGTCAAC TTGGGTTGTGTACGGAACCTGCCATCACAAAAAAGGTGAAGCACGGAGATCT AGAAGAGCTGTGACGCTCCCCTCCCATTCCACTAGGAAGCTGCAAACGCGGT CGCAAACCTGGTTGGAATCAAGAGAATACACAAAGCACTTGATTAGAGTCGA AAATTGGATATTCAGGAACCCTGGCTTCGCGTTAGCAGCAGCTGCCATCGCTT GGCTTTTGGGAAGCTCAACGAGCCAAAAAGTCATATACTTGGTCATGATACT GCTGATTGCCCCGGCATACAGCATCAGGTGCATAGGAGTCAGCAATAGGGAC TTTGTGGAAGGTATGTCAGGTGGGACTTGGGTTGATGTTGTCTTGGAACATGGAGGTTGTGTCACCGTAATGGCACAGGACAAACCGACTGTCGACATAGAGCT GGTTACAACAACAGTCAGCAACATGGCGGAGGTAAGATCCTACTGCTATGAG GCATCAATATCGGACATGGCTTCGGACAGCCGCTGCCCAACACAAGGTGAAG CCTACCTTGACAAGCAATCAGACACTCAATATGTCTGCAAAAGAACGTTAGT GGACAGAGGCTGGGGAAATGGATGTGGACTTTTTGGCAAAGGGAGCCTGGT GACATGCGCTAAGTTTGCATGCTCCAAGAAAATGACCGGGAAGAGCATCCAG CCAGAGAATCTGGAGTACCGGATAATGCTGTCAGTTCATGGCTCCCAGCACA GTGGGATGATCGTTAATGACACAGGACATGAAACTGATGAGAATAGAGCGAA GGTTGAGATAACGCCCAATTCACCAAGAGCCGAAGCCACCCTGGGGGGTTTT GGAAGCCTAGGACTTGATTGTGAACCGAGGACAGGCCTTGACTTTTCAGATT TGTATTACTTGACTATGAATAACAAGCACTGGTTGGTTCACAAGGAGTGGTTC CACGACATTCCATTACCTTGGCACGCTGGGGCAGACACCGGAACTCCACACT GGAACAACAAAGAAGCACTGGTAGAGTTCAAGGACGCACATGCCAAAAGG CAAACTGTCGTGGTTCTAGGGAGTCAAGAAGGAGCAGTTCACACGGCCCTT GCTGGAGCTCTGGAGGCTGAGATGGATGGTGCAAAGGGAAGGCTGTCCTCT GGCCACTTGAAATGTCGCCTGAAAATGGATAAACTTAGATTGAAGGGCGTGT CATACTCCTTGTGTACCGCAGCGTTCACATTCACCAAGATCCCGGCTGAAAC ACTGCACGGGACAGTCACAGTGGAGGTACAGTACGCAGGGACAGATGGACC TTGCAAGGTTCCAGCTCAGATGGCGGTGGACATGCAAACTCTGACCCCAGTT GGGAGGTTGATAACCGCTAACCCCGTAATCACTGAAAGCACTGAGAACTCTA AGATGATGCTGGAACTTGATCCACCATTTGGGGACTCTTACATTGTCATAGGA GTCGGGGAGAAGAAGATCACCCACCACTGGCACAGGAGTGGCAGCACCATT GGAAAAGCATTTGAAGCCACTGTGAGAGGTGCCAAGAGAATGGCAGTCTTG GGAGACACAGCCTGGGACTTTGGATCAGTTGGAGGCGCTCTCAACTCATTGG GCAAGGGCATCCATCAAATTTTTGGAGCAGCTTTCAAATCATTGTTTGGAGG AATGTCCTGGTTCTCACAAATTCTCATTGGAACGTTGCTGATGTGGTTGGGTC TGAACACAAAGAATGGATCTATTTCCCTTATGTGCTTGGCCTTAGGGGGAGTG TTGATCTTCTTATCCACAGCTGTCTCTGCTGATGTGGGGTGCTCGGTGGACTT CTCAAAGAAGGAGACGAGATGCGGTACAGGGGTGTTCGTCTATAACGACGTT GAAGCCTGGAGGGACAGGTACAAGTACCATCCTGACTCCCCCCGTAGATTGG CAGCAGCAGTCAAGCAAGCCTGGGAAGATGGTATCTGTGGGATCTCCTCTGT TTCAAGAATGGAAAACATCATGTGGAGATCAGTAGAAGGGGAGCTCAACGC AATCCTGGAAGAGAATGGAGTTCAACTGACGGTCGTTGTGGGATCTGTAAAA AACCCCATGTGGAGAGGTCCACAGAGATTGCCCGTGCCTGTGAACGAGCTG CCCCACGGCTGGAAGGCTTGGGGGAAATCGTACTTCGTCAGAGCAGCAAAG ACAAATAACAGCTTTGTCGTGGATGGTGACACACTGAAGGAATGCCCACTCAAACATAGAGCATGGAACAGCTTTCTTGTGGAGGATCATGGGTTCGGGGTATTT CACACTAGTGTCTGGCTCAAGGTTAGAGAAGATTATTCATTAGAGTGTGATCC AGCCGTTATTGGAACAGCTGTTAAGGGAAAGGAGGCTGTACACAGTGATCTA GGCTACTGGATTGAGAGTGAGAAGAATGACACATGGAGGCTGAAGAGGGCC CATCTGATCGAGATGAAAACATGTGAATGGCCAAAGTCCCACACATTGTGGA CAGATGGAATAGAAGAGAGTGATCTGATCATACCCAAGTCTTTAGCTGGGCC ACTCAGCCATCACAATACCAGAGAGGGCTACAGGACCCAAATGAAAGGGCC ATGGCACAGTGAAGAGCTTGAAATTCGGTTTGAGGAATGCCCAGGCACTAAG GTCCACGTGGAGGAAACATGTGGAACAAGAGGACCATCTCTGAGATCAACC ACTGCAAGCGGAAGGGTGATCGAGGAATGGTGCTGCAGGGAGTGCACAATG CCCCCACTGTCGTTCCGGGCTAAAGATGGCTGTTGGTATGGAATGGAGATAA GGCCCAGGAAAGAACCAGAAAGTAACTTAGTAAGGTCAATGGTGACTGCAG GATCAACTGATCACATGGATCACTTCTCCCTTGGAGTGCTTGTGATTCTGCTC ATGGTGCAGGAAGGGCTGAAGAAGAGAATGACCACAAAGATCATCATAAGC ACATCGATGGCAGTGCTGGTAGCTATGATCCTGGGAGGATTTTCAATGAGTGA CCTGGCTAAGCTTGCAATTTTGATGGGTGCCACCTTCGCGGAAATGAACACT GGAGGAGATGTAGCTCATCTGGCGCTGATAGCGGCATTCAAAGTCAGACCAG CGTTGCTGGTATCTTTCATCTTCAGAGCTAATTGGACACCCCGTGAAAGCATG CTGCTGGCCTTGGCCTCGTGTCTTTTGCAAACTGCGATCTCCGCCTTGGAAG GCGACCTGATGGTTCTCATCAATGGTTTTGCTTTGGCCTGGTTGGCAATACGA GCGATGGTTGTTCCACGCACTGATAACATCACCTTGGCAATCCTGGCTGCTCT GACACCACTGGCCCGGGGCACACTGCTTGTGGCGTGGAGAGCAGGCCTTGC TACTTGCGGGGGGTTTATGCTCCTCTCTCTGAAGGGAAAAGGCAGTGTGAAG AAGAACTTACCATTTGTCATGGCCCTGGGACTAACCGCTGTGAGGCTGGTCG ACCCCATCAACGTGGTGGGACTGCTGTTGCTCACAAGGAGTGGGAAGCGGA GCTGGCCCCCTAGCGAAGTACTCACAGCTGTTGGCCTGATATGCGCATTGGCT GGAGGGTTCGCCAAGGCAGATATAGAGATGGCTGGGCCCATGGCCGCGGTCG GTCTGCTAATTGTCAGTTACGTGGTCTCAGGAAAGAGTGTGGACATGTACATT GAAAGAGCAGGTGACATCACATGGGAAAAAGATGCGGAAGTCACTGGAAAC AGTCCCCGGCTCGATGTGGCGCTAGATGAGAGTGGTGATTTCTCCCTGGTGG AGGATGACGGTCCCCCCATGAGAGAGATCATACTCAAGGTGGTCCTGATGAC CATCTGTGGCATGAACCCAATAGCCATACCCTTTGCAGCTGGAGCGTGGTACG TATACGTGAAGACTGGAAAAAGGAGTGGTGCTCTATGGGATGTGCCTGCTCCCAAGGAAGTAAAAAAGGGGGAGACCACAGATGGAGTGTACAGAGTAATGAC TCGTAGACTGCTAGGTTCAACACAAGTTGGAGTGGGAGTTATGCAAGAGGG GGTCTTTCACACTATGTGGCACGTCACAAAAGGATCCGCGCTGAGAAGCGGT GAAGGGAGACTTGATCCATACTGGGGAGATGTCAAGCAGGATCTGGTGTCAT ACTGTGGTCCATGGAAGCTAGATGCCGCCTGGGACGGGCACAGCGAGGTGC AGCTCTTGGCCGTGCCCCCCGGAGAGAGAGCGAGGAACATCCAGACTCTGC CCGGAATATTTAAGACAAAGGATGGGGACATTGGAGCGGTTGCGCTGGATTA CCCAGCAGGAACTTCAGGATCTCCAATCCTAGACAAGTGTGGGAGAGTGATA GGACTTTATGGCAATGGGGTCGTGATCAAAAATGGGAGTTATGTTAGTGCCAT CACCCAAGGGAGGAGGGAGGAAGAGACTCCTGTTGAGTGCTTCGAGCCTTC GATGCTGAAGAAGAAGCAGCTAACTGTCTTAGACTTGCATCCTGGAGCTGGG AAAACCAGGAGAGTTCTTCCTGAAATAGTCCGTGAAGCCATAAAAACAAGA CTCCGTACTGTGATCTTAGCTCCAACCAGGGTTGTCGCTGCTGAAATGGAGGAAGCCCTTAGAGGGCTTCCAGTGCGTTATATGACAACAGCAGTCAATGTCAC CCACTCTGGAACAGAAATCGTCGACTTAATGTGCCATGCCACCTTCACTTCAC GTCTACTACAGCCAATCAGAGTCCCCAACTATAATCTGTATATTATGGATGAGG CCCACTTCACAGATCCCTCAAGTATAGCAGCAAGAGGATACATTTCAACAAG GGTTGAGATGGGCGAGGCGGCTGCCATCTTCATGACCGCCACGCCACCAGG AACCCGTGACGCATTTCCGGACTCCAACTCACCAATTATGGACACCGAAGTG GAAGTCCCAGAGAGAGCCTGGAGCTCAGGCTTTGATTGGGTGACGGATCATT CTGGAAAAACAGTTTGGTTTGTTCCAAGCGTGAGGAACGGCAATGAGATCG CAGCTTGTCTGACAAAGGCTGGAAAACGGGTCATACAGCTCAGCAGAAAGA CTTTTGAGACAGAGTTCCAGAAAACAAAACATCAAGAGTGGGACTTTGTCG TGACAACTGACATTTCAGAGATGGGCGCCAACTTTAAAGCTGACCGTGTCAT AGATTCCAGGAGATGCCTAAAGCCGGTCATACTTGATGGCGAGAGAGTCATT CTGGCTGGACCCATGCCTGTCACACATGCCAGCGCTGCCCAGAGGAGGGGG CGCATAGGCAGGAATCCCAACAAACCTGGAGATGAGTATCTGTATGGAGGTG GGTGCGCAGAGACTGACGAAGACCATGCACACTGGCTTGAAGCAAGAATGC TCCTTGACAATATTTACCTCCAAGATGGCCTCATAGCCTCGCTCTATCGACCTG AGGCCGACAAAGTAGCAGCCATTGAGGGAGAGTTCAAGCTTAGGACGGAGC AAAGGAAGACCTTTGTGGAACTCATGAAAAGAGGAGATCTTCCTGTTTGGCT GGCCTATCAGGTTGCATCTGCCGGAATAACCTACACAGATAGAAGATGGTGCT TTGATGGCACGACCAACAACACCATAATGGAAGACAGTGTGCCGGCAGAGG TGTGGACCAGACACGGAGAGAAAAGAGTGCTCAAACCGAGGTGGATGGAC GCCAGAGTTTGTTCAGATCATGCGGCCCTGAAGTCATTCAAGGAGTTTGCCG CTGGGAAAAGAGGAGCGGCTTTTGGAGTGATGGAAGCCCTGGGAACACTGC CAGGACACATGACAGAGAGATTCCAGGAAGCCATTGACAACCTCGCTGTGC TCATGCGGGCAGAGACTGGAAGCAGGCCTTACAAAGCCGCGGCGGCCCAAT TGCCGGAGACCCTAGAGACCATTATGCTTTTGGGGTTGCTGGGAACAGTCTC GCTGGGAATCTTTTTCGTCTTGATGAGGAACAAGGGCATAGGGAAGATGGGC TTTGGAATGGTGACTCTTGGGGCCAGCGCATGGCTCATGTGGCTCTCGGAAA TTGAGCCAGCCAGAATTGCATGTGTCCTCATTGTTGTGTTCCTATTGCTGGTG GTGCTCATACCTGAGCCAGAAAAGCAAAGATCTCCCCAGGACAACCAAATG GCAATCATCATCATGGTAGCAGTAGGTCTTCTGGGCTTGATTACCGCCAATGA ACTCGGATGGTTGGAGAGAACAAAGAGTGACCTAAGCCATCTAATGGGAAG GAGAGAGGAGGGGGCAACCATAGGATTCTCAATGGACATTGACCTGCGGCC AGCCTCAGCTTGGGCCATCTATGCTGCCTTGACAACTTTCATTACCCCAGCCG TCCAACATGCAGTGACCACTTCATACAACAACTACTCCTTAATGGCGATGGCC ACGCAAGCTGGAGTGTTGTTTGGTATGGGCAAAGGGATGCCATTCTACGCATGGGACTTTGGAGTCCCGCTGCTAATGATAGGTTGCTACTCACAATTAACACCC CTGACCCTAATAGTGGCCATCATTTTGCTCGTGGCGCACTACATGTACTTGATC CCAGGGCTGCAGGCAGCAGCTGCGCGTGCTGCCCAGAAGAGAACGGCAGCT GGCATCATGAAGAACCCTGTTGTGGATGGAATAGTGGTGACTGACATTGACA CAATGACAATTGACCCCCAAGTGGAGAAAAAGATGGGACAGGTGCTACTCAT AGCAGTAGCCGTCTCCAGCGCCATACTGTCGCGGACCGCCTGGGGGTGGGG GGAGGCTGGGGCCCTGATCACAGCGGCAACTTCCACTTTGTGGGAAGGCTC TCCGAACAAGTACTGGAACTCCTCTACAGCCACTTCACTGTGTAACATTTTTA GGGGAAGTTACTTGGCTGGAGCTTCTCTAATCTACACAGTAACAAGAAACGC TGGCTTGGTCAAGAGACGTGGGGGTGGAACAGGAGAGACCCTGGGAGAGA AATGGAAGGCCCGCTTGAACCAGATGTCGGCCCTGGAGTTCTACTCCTACAA AAAGTCAGGCATCACCGAGGTGTGCAGAGAAGAGGCCCGCCGCGCCCTCAA GGACGGTGTGGCAACGGGAGGCCATGCTGTGTCCCGAGGAAGTGCAAAGCTGAGATGGTTGGTGGAGCGGGGATACCTGCAGCCCTATGGAAAGGTCATTGAT CTTGGATGTGGCAGAGGGGGCTGGAGTTACTACGCCGCCACCATCCGCAAAG TTCAAGAAGTGAAAGGATACACAAAAGGAGGCCCTGGTCATGAAGAACCCA TGTTGGTGCAAAGCTATGGGTGGAACATAGTCCGTCTTAAGAGTGGGGTGGA CGTCTTTCATATGGCGGCTGAGCCGTGTGACACGTTGCTGTGTGACATAGGTG AGTCATCATCTAGTCCTGAAGTGGAAGAAGCACGGACGCTCAGAGTCCTCTC CATGGTGGGGGATTGGCTTGAAAAAAGACCAGGAGCCTTTTGTATAAAAGTG TTGTGCCCATACACCAGCACTATGATGGAAACCCTGGAGCGACTGCAGCGTA GGTATGGGGGAGGACTGGTCAGAGTGCCACTCTCCCGCAACTCTACACATGA GATGTACTGGGTCTCTGGAGCGAAAAGCAACACCATAAAAAGTGTGTCCACC ACGAGCCAGCTCCTCTTGGGGCGCATGGACGGGCCCAGGAGGCCAGTGAAA TATGAGGAGGATGTGAATCTCGGCTCTGGCACGCGGGCTGTGGTAAGCTGCG CTGAAGCTCCCAACATGAAGATCATTGGTAACCGCATTGAAAGGATCCGCAG TGAGCACGCGGAAACGTGGTTCTTTGACGAGAACCACCCATATAGGACATGG GCTTACCATGGAAGCTATGAGGCCCCCACACAAGGGTCAGCGTCCTCTCTAA TAAACGGGGTTGTCAGGCTCCTGTCAAAACCCTGGGATGTGGTGACTGGAGT CACAGGAATAGCCATGACCGACACCACACCGTATGGTCAGCAAAGAGTTTTC AAGGAAAAAGTGGACACTAGGGTGCCAGACCCCCAAGAAGGCACTCGTCA GGTTATGAGCATGGTCTCTTCCTGGTTGTGGAAAGAGCTAGGCAAACACAAA CGGCCACGAGTCTGTACCAAAGAAGAGTTCATCAACAAGGTTCGTAGCAATG CAGCATTAGGGGCAATATTTGAAGAGGAAAAAGAGTGGAAGACTGCAGTGG AAGCTGTGAACGATCCAAGGTTCTGGGCTCTAGTGGACAAGGAAAGAGAGC ACCACCTGAGAGGAGAGTGCCAGAGTTGTGTGTACAACATGATGGGAAAAA GAGAAAAGAAACAAGGGGAATTTGGAAAGGCCAAGGGCAGCCGCGCCATC TGGTATATGTGGCTAGGGGCTAGATTTCTAGAGTTCGAAGCCCTTGGATTCTT GAACGAGGATCACTGGATGGGGAGAGAGAACTCAGGAGGTGGTGTTGAAGGGCTGGGATTACAAAGACTCGGATATGTCCTAGAAGAGATGAGTCGCATACC AGGAGGAAGGATGTATGCAGATGACACTGCTGGCTGGGACACCCGCATCAG CAGGTTTGATCTGGAGAATGAAGCTCTAATCACCAACCAAATGGAGAAAGGG CACAGGGCCTTGGCATTGGCCATAATCAAGTACACATACCAAAACAAAGTGG TAAAGGTCCTTAGACCAGCTGAAAAAGGGAAGACAGTTATGGACATTATTTC GAGACAAGACCAAAGGGGGAGCGGACAAGTTGTCACTTACGCTCTTAACAC ATTTACCAACCTAGTGGTGCAACTCATTCGGAATATGGAGGCTGAGGAAGTT CTAGAGATGCAAGACTTGTGGCTGCTGCGGAGGTCAGAGAAAGTGACCAAC TGGTTGCAGAGCAACGGATGGGATAGGCTCAAACGAATGGCAGTCAGTGGA GATGATTGCGTTGTGAAGCCAATTGATGATAGGTTTGCACATGCCCTCAGGTT CTTGAATGATATGGGAAAAGTTAGGAAGGACACACAAGAGTGGAAACCCTC AACTGGATGGGACAACTGGGAAGAAGTTCCGTTTTGCTCCCACCACTTCAAC AAGCTCCATCTCAAGGACGGGAGGTCCATTGTGGTTCCCTGCCGCCACCAAGATGAACTGATTGGCCGGGCCCGCGTCTCTCCAGGGGCGGGATGGAGCATCCG GGAGACTGCTTGCCTAGCAAAATCATATGCGCAAATGTGGCAGCTCCTTTATT TCCACAGAAGGGACCTCCGACTGATGGCCAATGCCATTTGTTCATCTGTGCC AGTTGACTGGGTTCCAACTGGGAGAACTACCTGGTCAATCCATGGAAAGGG AGAATGGATGACCACTGAAGACATGCTTGTGGTGTGGAACAGAGTGTGGATT GAGGAGAACGACCACATGGAAGACAAGACCCCAGTTACGAAATGGACAGAC ATTCCCTATTTGGGAAAAAGGGAAGACTTGTGGTGTGGATCTCTCATAGGGC ACAGACCGCGCACCACCTGGGCTGAGAACATTAAAAACACAGTCAACATGG TGCGCAGGATCATAGGTGATGAAGAAAAGTACATGGACTACCTATCCACCCA AGTTCGCTACTTGGGTGAAGAAGGGTCTACACCTGGAGTGCTGTAAGCACCA ATCTTAGTGTTGTCAGGCCTGCTAGTCAGCCACAGCTTGGGGAAAGCTGTGC AGCCTGTGACCCCCCCAGGAGAAGCTGGGAAACCAAGCCTATAGTCAGGCC GAGAACGCCATGGCACGGAAGAAGCCATGCTGCCTGTGAGCCCCTCAGAGGACACTGAGTCAAAAAACCCCACGCGCTTGGAGGCGCAGGATGGGAAAAGA AGGTGGCGACCTTCCCCACCCTTCAATCTGGGGCCTGAACTGGAGATCAGCT GTGGATCTCCAGAAGAGGGACTAGTGGTTAGAGGAGACCCCCCGGAAAACG CAAAACAGCATATTGACGCTGGGAAAGACCAGAGACTCCATGAGTTTCCACC ACGCTGGCCGCCAGGCACAGATCGCCGAATAGCGGCGGCCGGTGTGGGG
SEQ ID NO：73
AHZ13508.1，来自波利尼西亚爆发的Zika病毒多聚蛋白(H/PF/2013)
MKNPKKKSGGFRIVNMLKRGVARVSPFGGLKRLPAGLLLGHGPIRMVLAIL AFLRFTAIKPSLGLINRWGSVGKKEAMEIIKKFKKDLAAMLRIINARKEKKRRG ADTSVGIVGLLLTTAMAAEVTRRGSAYYMYLDRNDAGEAISFPTTLGMNKCYI QIMDLGHMCDATMSYECPMLDEGVEPDDVDCWCNTTSTWVVYGTCHHKKGE ARRSRRAVTLPSHSTRKLQTRSQTWLESREYTKHLIRVENWIFRNPGFALAAAAI AWLLGSSTSQKVIYLVMILLIAPAYSIRCIGVSNRDFVEGMSGGTWVDVVLEHG GCVTVMAQDKPTVDIELVTTTVSNMAEVRSYCYEASISDMASDSRCPTQGEAY LDKQSDTQYVCKRTLVDRGWGNGCGLFGKGSLVTCAKFACSKKMTGKSIQPE NLEYRIMLSVHGSQHSGMIVNDTGHETDENRAKVEITPNSPRAEATLGGFGSLG LDCEPRTGLDFSDLYYLTMNNKHWLVHKEWFHDIPLPWHAGADTGTPHWNNK EALVEFKDAHAKRQTVVVLGSQEGAVHTALAGALEAEMDGAKGRLSSGHLKC RLKMDKLRLKGVSYSLCTAAFTFTKIPAETLHGTVTVEVQYAGTDGPCKVPAQ MAVDMQTLTPVGRLITANPVITESTENSKMMLELDPPFGDSYIVIGVGEKKITHH WHRSGSTIGKAFEATVRGAKRMAVLGDTAWDFGSVGGALNSLGKGIHQIFGAA FKSLFGGMSWFSQILIGTLLMWLGLNTKNGSISLMCLALGGVLIFLSTAVSADV GCSVDFSKKETRCGTGVFVYNDVEAWRDRYKYHPDSPRRLAAAVKQAWEDGI CGISSVSRMENIMWRSVEGELNAILEENGVQLTVVVGSVKNPMWRGPQRLPVP VNELPHGWKAWGKSYFVRAAKTNNSFVVDGDTLKECPLKHRAWNSFLVEDH GFGVFHTSVWLKVREDYSLECDPAVIGTAVKGKEAVHSDLGYWIESEKNDTWR LKRAHLIEMKTCEWPKSHTLWTDGIEESDLIIPKSLAGPLSHHNTREGYRTQMK GPWHSEELEIRFEECPGTKVHVEETCGTRGPSLRSTTASGRVIEEWCCRECTMPP LSFRAKDGCWYGMEIRPRKEPESNLVRSMVTAGSTDHMDHFSLGVLVILLMVQ EGLKKRMTTKIIISTSMAVLVAMILGGFSMSDLAKLAILMGATFAEMNTGGDVA HLALIAAFKVRPALLVSFIFRANWTPRESMLLALASCLLQTAISALEGDLMVLIN GFALAWLAIRAMVVPRTDNITLAILAALTPLARGTLLVAWRAGLATCGGFMLLS LKGKGSVKKNLPFVMALGLTAVRLVDPINVVGLLLLTRSGKRSWPPSEVLTAVG LICALAGGFAKADIEMAGPMAAVGLLIVSYVVSGKSVDMYIERAGDITWEKDA EVTGNSPRLDVALDESGDFSLVEDDGPPMREIILKVVLMTICGMNPIAIPFAAGA WYVYVKTGKRSGALWDVPAPKEVKKGETTDGVYRVMTRRLLGSTQVGVGVM QEGVFHTMWHVTKGSALRSGEGRLDPYWGDVKQDLVSYCGPWKLDAAWDG HSEVQLLAVPPGERARNIQTLPGIFKTKDGDIGAVALDYPAGTSGSPILDKCGRVI GLYGNGVVIKNGSYVSAITQGRREEETPVECFEPSMLKKKQLTVLDLHPGAGKT RRVLPEIVREAIKTRLRTVILAPTRVVAAEMEEALRGLPVRYMTTAVNVTHSGTE IVDLMCHATFTSRLLQPIRVPNYNLYIMDEAHFTDPSSIAARGYISTRVEMGEAA AIFMTATPPGTRDAFPDSNSPIMDTEVEVPERAWSSGFDWVTDHSGKTVWFVPS VRNGNEIAACLTKAGKRVIQLSRKTFETEFQKTKHQEWDFVVTTDISEMGANFK ADRVIDSRRCLKPVILDGERVILAGPMPVTHASAAQRRGRIGRNPNKPGDEYLY GGGCAETDEDHAHWLEARMLLDNIYLQDGLIASLYRPEADKVAAIEGEFKLRTE QRKTFVELMKRGDLPVWLAYQVASAGITYTDRRWCFDGTTNNTIMEDSVPAEVWTRHGEKRVLKPRWMDARVCSDHAALKSFKEFAAGKRGAAFGVMEALGTLP GHMTERFQEAIDNLAVLMRAETGSRPYKAAAAQLPETLETIMLLGLLGTVSLGI FFVLMRNKGIGKMGFGMVTLGASAWLMWLSEIEPARIACVLIVVFLLLVVLIPE PEKQRSPQDNQMAIIIMVAVGLLGLITANELGWLERTKSDLSHLMGRREEGATIG FSMDIDLRPASAWAIYAALTTFITPAVQHAVTTSYNNYSLMAMATQAGVLFGMG KGMPFYAWDFGVPLLMIGCYSQLTPLTLIVAIILLVAHYMYLIPGLQAAAARAAQ KRTAAGIMKNPVVDGIVVTDIDTMTIDPQVEKKMGQVLLIAVAVSSAILSRTAWG WGEAGALITAATSTLWEGSPNKYWNSSTATSLCNIFRGSYLAGASLIYTVTRNA GLVKRRGGGTGETLGEKWKARLNQMSALEFYSYKKSGITEVCREEARRALKD GVATGGHAVSRGSAKLRWLVERGYLQPYGKVIDLGCGRGGWSYYAATIRKVQE VKGYTKGGPGHEEPMLVQSYGWNIVRLKSGVDVFHMAAEPCDTLLCDIGESSS SPEVEEARTLRVLSMVGDWLEKRPGAFCIKVLCPYTSTMMETLERLQRRYGGG LVRVPLSRNSTHEMYWVSGAKSNTIKSVSTTSQLLLGRMDGPRRPVKYEEDVN LGSGTRAVVSCAEAPNMKIIGNRIERIRSEHAETWFFDENHPYRTWAYHGSYEA PTQGSASSLINGVVRLLSKPWDVVTGVTGIAMTDTTPYGQQRVFKEKVDTRVP DPQEGTRQVMSMVSSWLWKELGKHKRPRVCTKEEFINKVRSNAALGAIFEEEK EWKTAVEAVNDPRFWALVDKEREHHLRGECQSCVYNMMGKREKKQGEFGKA KGSRAIWYMWLGARFLEFEALGFLNEDHWMGRENSGGGVEGLGLQRLGYVL EEMSRIPGGRMYADDTAGWDTRISRFDLENEALITNQMEKGHRALALAIIKYTY QNKVVKVLRPAEKGKTVMDIISRQDQRGSGQVVTYALNTFTNLVVQLIRNMEA EEVLEMQDLWLLRRSEKVTNWLQSNGWDRLKRMAVSGDDCVVKPIDDRFAH ALRFLNDMGKVRKDTQEWKPSTGWDNWEEVPFCSHHFNKLHLKDGRSIVVPC RHQDELIGRARVSPGAGWSIRETACLAKSYAQMWQLLYFHRRDLRLMANAICS SVPVDWVPTGRTTWSIHGKGEWMTTEDMLVVWNRVWIEENDHMEDKTPVTK WTDIPYLGKREDLWCGSLIGHRPRTTWAENIKNTVNMVRRIIGDEEKYMDYLST QVRYLGEEGSTPGVL
SEQ ID NO：74
9320_Zika_PF_1F
ttaggatccGTTGTTGATCTGTGTGAAT
SEQ ID NO：75
9321_Zika_PF_1R
taactcgagCGTACACAACCCAAGTT
SEQ ID NO：76
9322_Zika_PF_2F
ttaggatccTCACTAGACGTGGGAGTG
SEQ ID NO：77
9323_Zika_PF_2R
taactcgagAAGCCATGTCYGATATTGAT
SEQ ID NO：78
9324_Zika_PF_3F
ttaggatccGCATACAGCATCAGGTG
SEQ ID NO：79
9325_Zika_PF_3R
taactcgagTGTGGAGTTCCGGTGTCT
SEQ ID NO：80
9326_Zika_PF_4F
ttaggatccGAATAGAGCGAARGTTGAGATA
SEQ ID NO：81
9327_Zika_PF_4R
taactcgAGTGGTGGGTGATCTTCTTCT
SEQ ID NO：82
9328_Zika_PF_5F
ttaggatcCAGTCACAGTGGAGGTACAGTAC
SEQ ID NO：83
9329_Zika_PF_5R
taactcgagCRCAGATACCATCTTCCC
SEQ ID NO：84
9330_Zika_PF_6F
ttaggatCCCTTATGTGCTTGGCCTTAG
SEQ ID NO：85
9331_Zika_PF_6R
taactcgagTCTTCAGCCTCCATGTG
SEQ ID NO：86
9332_Zika_PF_7F
ttaggatccAATGCCCACTCAAACATAGA
SEQ ID NO：87
9333_Zika_PF_7R
taactcgagTCATTCTCTTCTTCAGCCCTT
SEQ ID NO：88
9334_Zika_PF_8F
ttaggatccAAGGGTGATCGAGGAAT
SEQ ID NO：89
9335_Zika_PF_8R
taactcgagTTCCCTTCAGAGAGAGGAGC
SEQ ID NO：90
9336_Zika_PF_9F
ttaggatccTCTTTTGCAAACTGCGATC
SEQ ID NO：91
9337_Zika_PF_9R
taactcgagTCCAGCTGCAAAGGGTAT
SEQ ID NO：92
9338_Zika_PF_10F
ttaggatccGTGTGGACATGTACATTGA
SEQ ID NO：93
9339_Zika_PF_10R
taactcgagCCCATTGCCATAAAGTC
SEQ ID NO：94
9340_Zika_PF_11F
ttaggatccTCATACTGTGGTCCATGGA
SEQ ID NO：95
9341_Zika_PF_11R
taactcgagGCCCATCTCAACCCTTG
SEQ ID NO：96
9342_Zika_PF_12F
ttaggatccTAGAGGGCTTCCAGTGC
SEQ ID NO：97
9343_Zika_PF_12R
taactcgAGATACTCATCTCCAGGTTTGTTG
SEQ ID NO：98
9344_Zika_PF_13F
ttaggatccGAAAACAAAACATCAAGAGTG
SEQ ID NO：99
9345_Zika_PF_13R
taactcgagGAATCTCTCTGTCATGTGTCCT
SEQ ID NO：100
9346_Zika_PF_14F
ttaggatccTTGATGGCACGACCAAC
SEQ ID NO：101
9347_Zika_PF_14R
ttaggatccGTTGTTGATCTGTGTGAAT
SEQ ID NO：102
9348_Zika_PF_15F
taactcgagCAGGTCAATGTCCATTG
SEQ ID NO：103
9349_Zika_PF_15R
ttaggatccTGTTGTGTTCCTATTGCTGGT
SEQ ID NO：104
9350_Zika_PF_16F
taactcgaGTGATCAGRGCCCCAGC
SEQ ID NO：105
9351_Zika_PF_16R
ttaggatccTGCTGCCCAGAAGAGAA
SEQ ID NO：106
9352_Zika_PF_17F
taactcgaGCACCAACAYGGGTTCTT
SEQ ID NO：107
9353_Zika_PF_17R
ttaggatcCTCAAGGACGGTGTGGC
SEQ ID NO：108
9354_Zika_PF_18F
taactcgagCAATGATCTTCATGTTGGG
SEQ ID NO：109
9355_Zika_PF_18R
ttaggatccTATGGGGGAGGACTGGT
SEQ ID NO：110
9356_Zika_PF_19F
taactcGAGCCCAGAACCTTGGATC
SEQ ID NO：111
9357_Zika_PF_19R
ttaggatcCAGACCCCCAAGAAGGC
SEQ ID NO：112
9358_Zika_PF_20F
taactcgagCCCCTTTGGTCTTGTCT
SEQ ID NO：113
9359_Zika_PF_20R
ttaggatccAGGAAGGATGTATGCAGATG
SEQ ID NO：114
9360_Zika_PF_21F
taactcgagACATTTGCGCATATGATTTTG
SEQ ID NO：115
9361_Zika_PF_21R
ttaggatccAGGAAGGACACACAAGAGT
SEQ ID NO：116
9362_Zika_PF_22F
taactcgagACAGGCTGCACAGCTTT
SEQ ID NO：117
9363_Zika_PF_22R
ttaggatccTCTCTCATAGGGCACAGAC
在一些实施方式中，Zika病毒具有多蛋白，包括具有由SEQ ID NO：14-69或 72中的任一个提供的氨基酸序列的包膜(E)蛋白。在一些实施方式中，多蛋白或 E蛋白序列与SEQ ID NO：2-69或72中的任一个至少80％、81％、82％、83％、 84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、 97％、98％、99％、99.5％、99.6％、99.7％、99.8％或99.9％相同。
在两个或更多个核酸或氨基酸序列的情况中，术语“相同”或百分比“同一 性”是指两个或更多个相同的序列或子序列。如果两个序列具有的相同氨基酸残 基或核苷酸的特定百分比(例如至少80％、85％、90％、95％、96％、97％、98％ 或99％同一性)在特定区域或整个序列上进行比较和比对以获得比较窗口或指定 区域上的最大对应性，如使用以下序列比较算法之一或通过人工比对和目视检查 所测量的。任选地，同一性存在于长度为至少约50个核苷酸(或10个氨基酸) 的区域上，或更优选地在长度为100至500或1000个以上的核苷酸(或20、50、 200或更多个氨基酸)的区域上。在一些实施方式中，同一性存在于蛋白质的长度 上，例如E蛋白。
对于序列比较，通常一个序列作为参考序列，将测试序列与其进行比较。用 于比较的序列比对方法在本领域中是公知的。参见，例如，Smith和Waterman(1970)Adv.Appl.Math.2:482c的局部同源性算法，通过Needleman和Wunsch J.Mol.Biol. 48:443,1970的同源性比对算法，通过Pearson和Lipman.Proc.Natl.Acad.Sci.USA 85:2444,1988的搜索相似性方法，通过使用这些算法的计算机化实现(GAP， BESTFIT，FASTA，Jalview和TFASTA，在威斯康星遗传学软件包，Genetics Computer Group.575ScienceDr.,Madison.WI)，通过使用例如CLUSTALW(Larkin 等人，(2007).Bioinformatics，23,2947-2948.)或MAFFT(Katoh&Toh2008 Briefings in Bioinformatics 9：286-298)的多序列比对实施，或通过人工比对和目 测检查(参见例如Brent Current Protocols inMolecular Biology，John Wiley&Sons， Inc.(Ringbou ed.，2003))。适用于确定序列同一性百分比和序列相似性的算法的 两个示例是BLAST和BLAST 2.0算法，其分别在Altschul等，Nuc.Natl.Acad.Sci. USA，Acids Res.25：3389-3402,1977和Altschul等，J.Mol.Biol.215：403-410,1990 中描述。
实施例
实施例1：生产适合用作人和动物疫苗的Zika药物
材料和方法：
为了生产ZikaV，使用JEV加工平台(Srivastava等，Vaccine 19(2001) 4557-4565；US 6,309,650B1)作为基础。某些过程步骤的微小变化适用于ZikaV 特性并提高纯度。过程步骤的简要概述如下(参见图9A和图9B)。简而言之，在 Zika病毒的纯化过程中评估了硫酸鱼精蛋白(PS)的意想不到的和新颖的纯化性 质。如图10所示，非感染性病毒颗粒聚集体、HCP和其它LMW杂质通过PS沉 淀除去，如通过在SEC-HPLC中去除聚集肩部所示并且通过PS处理而没有感染性 病毒滴度损失。下面提供了Zika纯化方案的进一步优化。
上游：
·基于滚瓶的Vero细胞扩增(25x850cm2CellBind)：
·5％CO2，35℃，MEM+2mM L-谷氨酰胺+10％FBS
·在MOI 0.01下感染ZikaV研究硕士种子库(rMSB)
·无血清病毒生产
·5％CO2，35℃，MEM+2mM L-谷氨酰胺
·具有重新进料的多次收获(第2、3、5和7天)
·无菌过滤收获物并在2-8℃储存直至进一步加工
下游：
·通过超滤(100kDa)合并收获物和浓缩物
·如果需要，可以稳定浓缩的收获物(Tris/10％蔗糖)用于储存(-80℃)
·通过硫酸鱼精蛋白(2mg/mL)去除hcDNA
·蔗糖梯度纯化(优化三层梯度)
·甲醛灭活(0.02％，22℃，10天)，用焦亚硫酸钠中和
·稀释至DS抗原靶标含量和具有氢氧化铝(0.5mg Al/mL)的制剂
来自法属波利尼西亚的Zika病毒株H/PF/2013最初是从51岁的女性(登录号KJ776791.1，本文中也是SEQ ID NO：13)中分离出来的。样品获自欧洲病毒档 案(EVAg；Ref-SKU：001v-EVA1545)。基于此材料，在Vero细胞上制备研究主种 子库(rMSB)作为细胞底物，并通过测序检查基因组序列。由于Zika病毒株 H/PF/2013的5'和3'侧翼序列的基因组序列是未知的，所以在基于其它Zika病毒株 的那些区域中设计了用于测序的引物，而内部引物根据公开的序列(SEQ ID NO： 74至117，也参见表A)设计。通过使用这些引物由rMSB获得的序列由SEQ ID NO： 72提供。该序列与公布的Zika病毒株H/PF/2013(SEQ ID NO：13)的序列有100％ 重叠。然而，我们测序了SEQ ID NO：72中所示的另外5'区域(另外的40bp)和 3'区域(另外的160bp)。在一个优选的实施方式中，本发明的Zika病毒包含SEQ ID NO：72。基因组RNA比根据SEQ ID NO：72的序列稍长(可能是另外的200bp)。 此外，适用于宿主细胞例如Vero细胞的Zika病毒预期可能含有一个或多个突变。 由于这些原因，本发明的Zika病毒包含SEQ ID NO：72的序列或优选是与SEQ ID NO：72的序列具有至少95％、96％、97％、98％或至少99％序列同一性的序列。 此外，因为病毒基因组可能甚至含有SEQ IDNO：72的更多侧翼区域；在一个实 施方式中，本发明的Zika病毒含有SEQ ID NO：72的序列，并且任选地在5'和/ 或3'末端进一步包含至少10、至少20、至少30、至少40、至少50、至少60、至 少70、至少80、至少90、至少100、至少110、至少120或至少130个核苷酸的 延伸。在一个优选的实施方式中，Zika病毒至少包含本发明的Zika病毒株 H/PF/2013的全部多蛋白质的编码序列，即SEQ ID NO：73的氨基酸序列或多蛋 白质，其与SEQ ID NO：73提供的序列具有至少95％、96％、97％、98％或至少 99％的序列同一性。此外，Zika病毒至少包含本发明SEQ ID NO：47的Zika病毒 H/PF/2013的E-蛋白的编码序列或其与SEQ ID NO：47提供的序列具有至少95％、 96％、97％、98％或至少99％的序列同一性的E蛋白。
在Vero细胞上的病毒生长
Vero细胞在含有10％胎牛血清(FBS)的Eagle最小基本培养基(EMEM)中 生长。Vero细胞单层的滚瓶培养物以每个细胞0.01个斑块形成单位(pfu)的感染 复数(moi)用Zika病毒株H/PF/2013感染。病毒吸附2小时后，将培养物用PBS 洗涤3次并用不含FBS的EMEM进料，并在+35℃和5％CO2下温育。温育感染的 Vero细胞培养物直至病毒滴度达到期望水平。
在第2、3、5和7天收获培养基并从这些收获日汇集，然后在标准离心机中 离心。然后过滤上清液。通过TFF超滤浓缩病毒培养物上清液以除去细胞培养基 成分并减少批量。
收获程序的评估
当前的JEV收获过程在感染后的第3、5、7和9天具有预定收获。为了模拟 JEV过程，在感染培养基(含有2％FBS的MEM+2mM L-谷氨酰胺)中以0.01的 MOI用ZIKV库P4-FBS感染滚瓶2小时。除去接种物后，将细胞用PBS洗涤两 次并加入200mL生产培养基(MEM+2mM L-谷氨酰胺)。
在第2天取样后，在感染后第3天进行第一次病毒收获。此时，与在第2天 除去病毒的细胞相比，可以观察到显著更高的CPE。斑块测定分析显示第2天的 病毒滴度与标准收获计划相同。然而，从第3天收获开始，观察到的滴度显著较 低，与标准收获计划相比观察到的CPE增加。在感染后第5天，根本没有观察到 更多的活细胞，并且实验终止于最后的第5天收获。
表3：每个斑块测定的计算滴度汇总在下表中。
Figure BDA0003695595590001121
Figure BDA0003695595590001131
这一发现导致了优化的收获计划，以更好地控制CPE并允许额外的收获第5 天和第7天，参见图15。对于两个收获日，与修改的方案相比，优化的ZikaV方 案产生显著更高的病毒滴度，显示出第一次收获对于产量是至关重要的。另外在 第3天的第一次收获导致最多2个收获点，而在第2天的第一次收获允许4个收 获点以进一步增加产量获取。
Zika病毒的下游纯化
除非另有说明，纯化过程在室温(18-22℃)下进行。通过使用100kDa截止 TFF超滤模块浓缩过滤的组合收获物而开始病毒纯化，以去除细胞培养基组分并 减少批量。浓缩后，使用两种组分的储液将汇集的过滤收获物质调整至终浓度为 25mM Tris pH 7.5和10％蔗糖(w/w)(参见图11，PS处理前对于不同收获物的 SEC-HPLC)。如果需要，这可以在<-65℃冷冻浓缩的收获物。
通过用硫酸鱼精蛋白(2mg/mL)沉淀，接着进行蔗糖密度离心(2-8℃)作为 最终的抛光步骤，以实现宿主细胞DNA和蛋白质减少以及浓缩材料中非感染性病 毒聚集体的减少(参见图20，PS处理后对于不同收获物的SEC-HPLC)。纯化过 程设计为在2天内完成，SGC从第1天结束开始，随后在第2天进行分馏和 SDS-PAGE分析。蔗糖梯度级分在SDS-PAGE分析期间保存在2-8℃(银染)以鉴 定含有ZikaV的纯级分(参见图21)。汇集相关级分后，用福尔马林稀释并灭活该 汇集物。汇集蔗糖梯度离心的相关级分后，将汇集物在PBS中以1:3稀释并通过 福尔马林(0.02％v/v，200ppm)灭活。通过SDS-PAGE对级分进行分析。
PS处理对病毒恢复的影响
通过SEC-HPLC和斑块测定进行PS处理之前(图11)和之后(图12)分析 第2、3、5和7天的单个30x浓缩收获物的样品。SEC-HPLC用于测定表示为峰 面积的相对总ZikaV含量(活性+非活性)，而相对ZikaV峰值纯度是作为病毒单 体群体相对于总病毒峰值的相对含量给出的。斑块测定表明每个样品中总活性病 毒颗粒的含量。实验结果总结在表4中。通过SEC-HPLC的病毒峰回收率仅在12％ 至36％之间，PS处理后的峰值纯度在>90％范围内(未检测到病毒聚集体)。通过 斑块测定回收的活性病毒颗粒全部>100％(130-700％，在测定变异性范围内)，表 明PS处理过程中没有活性病毒颗粒丢失。这些结果表明，在PS处理期间，仅去 除了非感染性(未成熟和/或聚集的病毒)颗粒。
表4：在PS处理之前和之后通过SEC-HPLC和斑块测定进行的ZikaV回收。
SEC-HPLC
Figure BDA0003695595590001132
Figure BDA0003695595590001141
斑块测定
Figure BDA0003695595590001142
蔗糖梯度离心
将PS处理的收获物分成两部分并装载在两个离心瓶上。蔗糖密度梯度离心 (SGC)用于ZikaV材料的最终浓缩和抛光。将ZikaV PS处理的浓缩收获物装载 在由三层不同密度的蔗糖组成的溶液的顶部。基于初步研究选择三个蔗糖层，如 ChikV所示(数据未显示)，其显示线性蔗糖梯度的形成和病毒颗粒与残余污染物 的完全分离。蔗糖溶液的最佳体积由经验确定。表5中示出了在100mL瓶规模中 离心的单个层的体积。
表5：瓶中离心的单个层/体积。
Figure BDA0003695595590001143
通过分层单个蔗糖层来制备蔗糖梯度瓶。一根塑料管连接在蠕动泵管上。使 用夹具将塑料管安装在实验室支架上，并放入离心瓶中。塑料管的喷嘴接触瓶子 的底部。使用蠕动泵将ZikaV材料和蔗糖溶液泵入圆筒中。量筒用作进料容器。 第一个溶液泵是ZikaV材料，因为它代表了最低密度(10％蔗糖(w/w))的溶液。 在ZikaV材料之后，以升序泵入蔗糖溶液，以15％(w/w)溶液J开始，随后35％ 蔗糖溶液I，并以最高密度蔗糖溶液H(50％(w/w))结尾。所描述的设置在图14 中示出。在转移所有蔗糖溶液之后，小心移除塑料管以免干扰各层。
离心之前，将离心机预冷至4℃。将准备好的SG瓶小心地转移到预冷的转子 中。(注意：为避免干扰蔗糖层，必须避免在转移到转子时突然移动瓶子)。将瓶 子在4℃以～11.000 RCF max离心至少20小时，不启动制动/减速。在使用具有不 同转子的不同离心机系统的情况下，需要基于k系数计算必要的速度和离心时间 以实现可比较的离心效率。
采用蠕动泵手动进行蔗糖梯度的收获。使用连接到蠕动泵管的塑料管来收集 蔗糖梯度。使用夹具将包含梯度的瓶子以倾斜的位置(～12°)安装到实验室支架 上。然后将塑料管放入与瓶子底部边缘接触的瓶子中，并用夹子固定在适当位置。 这导致管道入口和瓶底部之间的1-2mm小间隙(参见图14)。
使用设定为每分钟30mL流速的蠕动泵，收获梯度并手动分成2mL级分。收 获每瓶32个级分的总数(约64mL)，并丢弃剩余的体积。立即通过SDS-PAGE/ 银染检测级分以鉴定含有足够高纯度的含病毒级分。代表性SDS-PAGE显示于图 14中。汇集级分10-14并进一步处理。
通过在22℃控制温度的培养箱中在10天的时间内用0.02％甲醛培养使纯化的 病毒溶液灭活。在第十天加入焦亚硫酸钠以将甲醛中和。
在PETG容器中，用PBS将蔗糖梯度汇集物(取样后～17mL)进一步稀释3 倍至最终体积51mL。向该汇集物中加入相当于预甲醛汇集物最终体积的1/50的 1％甲醛(10000ppm)溶液体积，导致有效浓度为200ppm。甲醛处理的溶液在磁 力搅拌器上混合10分钟。取样后，将甲醛处理的病毒溶液置于22℃±2℃的冷却 培养箱内。在添加甲醛后的第5天，将经甲醛处理的病毒溶液通过0.2μm过滤器 过滤，然后再次置于22℃±2℃的培养箱中。在第10天，在去除10天灭活最终样 品后，将200mM焦亚硫酸钠溶液(2mM终浓度)的1％(最终甲醛处理的病毒溶 液的重量)体积无菌转移到含有经甲醛处理的病毒溶液的PETG容器中。在磁力搅拌器上混合5分钟后，将中和的灭活病毒溶液在室温(20至25℃)下保持至少 30分钟。取样后，将中和的灭活病毒溶液储存在5℃±3℃直至进一步处理。
通过甲醛灭活
该步骤的关键参数是最终福尔马林浓度、温度、混合和转移到新容器中。已 经在稀释汇集物预甲醛处理中设定了最大pfu/mL(通过斑块测定确定)的初步验 收标准。
通过以下参数监测中和的灭活病毒溶液的质量：在第10天的斑块测定， SEC-HPLC，SDS-PAGE/Western印迹。
有趣的是，在灭活期间采取的样品的SEC-HPLC分析随后用亚硫酸氢盐中和， 在整个灭活期间显示或多或少恒定的峰面积。这与使用由Srivastava等人的Vaccine 19(2001)4557-4565中公开的方法观察到病毒颗粒损失高达60％的JEV相反。在 规模缩小的模型中，由于较小规模的表面/面积比和由于非特定吸附导致的高损失， 病毒损失甚至更高。ZikaV灭活实验和JEV灭活的差异被注意到如下：
A)与JEV相比，ZikaV SGP汇集物关于残余PS(<2μg/mL)的纯度更高。 因此，3倍ZikaV灭活样品含有<<1μg/mL残余PS。市售JEV SGP汇集物平均含 有～120μg/mL(可能高达152μg/mL)。对灭活溶液的平均稀释度～14倍导致残余PS 含量高达～11μg/mL。可能是由于与福尔马林交联/反应，较高量的残余PS会引起 病毒沉淀。
B)ZikaV灭活样品含有～10％蔗糖(含～30-35％蔗糖的SGP汇集物的3倍稀释液)。在用福尔马林处理期间，蔗糖可能具有病毒ZikaV颗粒的稳定作用。
稀释到DS和氢氧化铝制剂(DP)
为了制备在小鼠效能测定中使用的ZikaV药物，与Ixiaro相比靶向5倍的抗 原含量(表示为总病毒颗粒或SEC峰面积)。确定抗原含量的基础是SEC-HPLC。 简而言之，以1ml/min和25℃用PBS+250mM NaCl、pH 7.4平衡的Superose 6 10/300 增量柱(GE Healthcare)用于检测收获样品和整个下游过程中214nm检测波长处 的ZikaV。在目前的JEV过程中，NIV中的抗原含量由特定的ELISA确定。在通 过ELISA和SEC-HPLC确定的抗原含量之间观察到良好的相关性。平均而言，市 售NIV样品中的抗原含量在33AU/mL的范围内，对应于～5.2mAU JEV峰面积， 参见图21。
ZikaV NIV第10天(Zika峰～36mAU，在Waters HPLC/Superose6Increase柱 上分析)用PBS稀释至6.3(～5.7x稀释)的目标。加入氢氧化铝至最终浓度为 0.5mg/mL铝(添加1/20v/v明矾2％储液)以制备ZikaV药物产品(DP)。将DP 轻轻混合5分钟。取出等分试样的DP，通过离心沉淀明矾并通过SEC-HPLC分析 澄清的上清液。在上清液中未检测到ZikaV峰，表明在矿物佐剂上完全吸附(估 计>95％)病毒颗粒。配制的ZikaV DP在2-8℃下储存。
灭活的Zika病毒DS的杂质谱与JEV DS的谱相当，但PS含量较低(表6)。
表6：Zika和JEV DS样品中杂质分布的测定：
Figure BDA0003695595590001161
Figure BDA0003695595590001171
*根据Srivastava等人的Vaccine 19(2001)4557-4565中公开的方案生产的JEV样品中的典型PS杂质。
SEC-MALLS结果
ZikaV NIV在214nm检测波长下的代表性SEC-HPLC洗脱曲线如图16所示。 注意的是，BSA(50μg/mL)被添加到样品中，以最大程度减少HPLC玻璃瓶由于 非特异性表面吸附造成的损失。估计ZikaV单体含量约为98％，多聚体含量为～2％。
样品的SEC-MALLS分析(图17)证实了单体ZikaV群体峰1的半径Rz为 21.6nm，多聚体峰2为～49nm。累积粒度分布显示89％的所有病毒颗粒在18到25nm 之间的半径范围内(图18)。
结果证实了ZikaV NIV的纯度和均一性。
通过斑块测定的病毒滴度
表7：在整个过程中通过斑块测定对活性ZikaV pfus进行定量。
样品 Pfu/mL
收获第2天(过滤) 6.4x10<sup>7</sup>
收获第3天(过滤) 1.0x10<sup>8</sup>
收获第5天(过滤) 1.5x10<sup>8</sup>
收获第7天(过滤) 1.1x10<sup>8</sup>
PS处理的收获物300x浓缩物(＝SGP上样) 9.0x10<sup>8</sup>
SGP汇集物 8.9x10<sup>8</sup>
灭活开始(SGP汇集物以1:3稀释) 3.4x10<sup>8</sup>
灭活第5天 <LOD
灭活第10天 <LOD
PS和Benzonase在工艺性能上的比较
对浓缩的ZikaV收获汇集物的DNA去除方法进行了直接比较。一份用PS (2mg/mL，室温15min)处理，另一份用Benzonase(50U/mL，2mM MgCl2，4 小时RT，48h 2-8℃)处理。如本报告中所述，两种样品均通过蔗糖梯度进一步纯 化。有趣的是，在经处理的ZikaV收获物的蔗糖梯度离心后，Benzonase处理的样 品没有产生任何纯级分。在检测到特定病毒条带的那些部分中，在整个收集的级 分中检测到高量的宿主细胞蛋白质。如预期的那样，PS处理的材料产生含纯ZikaV 的级分。这一发现可能表明，PS不仅可以有效去除沉淀中的DNA；另外它通过破 坏DNA(片段)和病毒颗粒的相互作用来提高梯度中病毒颗粒的回收率。Benzonase 处理不会去除DNA，只会导致其碎裂。残余的DNA片段可能仍然与病毒颗粒和 残余的HCP相互作用，导致蔗糖梯度中的交叉污染和共同纯化。汇集的SGP级分 也通过SEC-HPLC分析。虽然检测到很大的峰值，但SDS-PAGE证实该样品被HCP 高度污染。SEC-HPLC分析后，在UV214和280nm处可能检测到较大的峰，这是 由于HCP与大型病毒颗粒之间可能发生相互作用，从而改变了紫外吸光度。
Vero生长的Zika病毒的免疫原性
小鼠免疫
在免疫之前，将10只6周龄雌性CD1小鼠的组通过面部静脉血管放血并制备 免疫前血清。施用200μL的一次腹膜内免疫。用最终浓度为0.7％的氢氧化铝 (Al(OH)3)配制灭活Zika病毒的剂量滴定(12μg、3μg、1μg、0.33μg、0.11μg、 0.037μg和0.012μg，相当于IXIARO中的蛋白量)。免疫后三周，收集血液并制备 免疫血清。所有的动物实验均按照奥地利法律进行(BGB1Nr.501/1989)并经 “Magistratsabteilung 58”批准。
斑块减少中和试验(PRNT)
PRNT使用十二孔板。每孔用含有4×105个Vero细胞的1mL培养基接种，并 在5％CO2中温育35℃过夜。每个剂量组的热灭活血清汇集物一式三份进行测试。 将靶病毒(H/PF/2013(SEQ ID NO：13)或MR766(SEQ ID NO：11))稀释至 100pfu/165μL。等体积的靶病毒和血清稀释液在35℃和5％CO2下温育1小时。从 Vero细胞中吸出细胞培养基，向每个孔中加入330μL混合物靶病毒/血清稀释液， 将平板来回摇动5次，然后在35℃和5％CO2下温育2小时。向每个孔中加入1mL 含有EMEM和营养素的2％甲基纤维素溶液，然后将平板在35℃下用5％CO2温育 5天，然后用结晶紫/5％甲醛染色细胞1小时，随后用去离子水洗涤3次。将板空气干燥并手动计数每个孔中的斑块数量。
结果
对于亚洲(H/PF/2013)和非洲(MR766)谱系(分别为图19和图20)的Zika 病毒，在用灭活Zika病毒疫苗(H/PF/2013)免疫的小鼠的血清库中观察到中和低 至37ng(剂量相当于
Figure BDA0003695595590001181
中蛋白量)。在1:20血清稀释时观察到完全抑制， 免疫剂量低至110ng(剂量相当于
Figure BDA0003695595590001182
中蛋白量)。Zika病毒的亚洲 (H/PF/2013)和非洲(MR766)谱系的中和是相同的，这表明灭活Zika病毒疫苗 (H/PF/2013)的不同Zika病毒株之间的高度交叉中和。
使用Larocca等人(2016，Nature doi：10.1038/nature18952)描述的微量中和 测定进行另一种中和测定。发现本发明的灭活Zika病毒在1μg灭活的纯化病毒中 具有90的MN50(微中和)滴度。
进一步方法：使用Zika感染的小鼠模型评估灭活Zika病毒制剂的免疫原性。 使用500、50或5ng灭活Zika病毒与佐剂(例如含有或不含
Figure BDA0003695595590001183
的氢氧化铝) 或无佐剂皮下(s.c.)免疫成年小鼠组。另一组小鼠接受PBS作为阴性对照。每组 在t＝0时施用指定的接种物，并且在一些情况下也在3至4周后(t＝3/4)时施用。 在最后一次免疫接种后约三周开始，每隔一定时间从每只小鼠获得血清样品。使 用PRNT测试血清样品的中和抗体的存在。
还使用Zika感染的小鼠模型即IFN-α/β受体敲除小鼠(A129)评估灭活Zika 病毒制剂的体内保护效力(参见例如Dowall等，2016年3月4日， http://dx.doi.org/10.1101/042358)，或通过向C57BL/6或BALB/c小鼠施用抗 -IFN-α/β受体单克隆抗体来阻断IFN-α/β受体(参见例如Pinto等2011年12月7 日，DOI：10.1371/journal.ppat.1002407)。对于保护性测定，在t＝0时，将具有佐 剂(氢氧化铝)或不具有佐剂的灭活Zika病毒皮下接种到10只3-8周龄的A129、 C57BL/6或BALB/c小鼠组的后躯中。年龄匹配的对照用在明矾中的PBS或非特 异性抗原接种。任选地在第三至第四周后第二次施用所示接种以加强小鼠。然后 通过接种致命剂量的活Zika病毒在免疫后3至8周将小鼠皮下攻击。在攻击 C57BL/6和BALB/c小鼠前一天，它们被动地(腹膜内)施用抗-IFN-α/β受体单克 隆抗体。每天监测攻击小鼠的发病率和死亡率至多二十一天。另一种替代是攻击 颅内成年接种疫苗/未接种疫苗的成年小鼠并观察保护。
预期本发明方法产生的Zika病毒将在体外、体内和最终人体试验中提供与欧 盟和美国以及其它地方目前许可的JEV疫苗
Figure BDA0003695595590001191
非常相似的功能读数。剂量 可能会改变，但由于非常相似的杂质谱和几乎相同的生产，将预计与目前许可的 JEV疫苗(在欧盟和美国以及其它地区许可)所确定的非常相似的功效和安全性 结果。
讨论与结论
现有的用于生产灭活JEV疫苗
Figure BDA0003695595590001194
的制造平台被用作灭活ZikaV疫苗候 选者(亚洲菌株H/PF/2013)的制造可行性研究的基础。在滚瓶中培养的Vero细 胞上产生病毒。通过PS处理然后用优化蔗糖梯度纯化病毒。通过福尔马林处理进 行灭活(0.02％，22℃，10天)。对于小鼠中的探索性免疫研究，制备了与明矾一 起配制的DP，其与市售JEV疫苗
Figure BDA0003695595590001193
相比具有估计5倍高的病毒颗粒含量。 DS的杂质分布符合
Figure BDA0003695595590001192
(市售JEV疫苗)规范中定义的所有标准。Zika病 毒的亚洲(H/PF/2013)和非洲(MR766)谱系的中和是相同的，这表明灭活Zika 病毒疫苗(H/PF/2013)的不同Zika病毒株之间的高度交叉中和。
关于本发明的灭活Zika病毒疫苗的免疫原性的体内数据表明，该病毒具有惊 人的有效免疫原性，并且还具有高度的交叉保护性(非洲和亚洲菌株非常相似的 免疫原性)。数据表明免疫原性比最近报道的灭活Zika病毒疫苗候选者更高 (Larocca等，2016，同上)。灭活病毒是最安全的疫苗之一，特别优选用于递送 给安全性特别关注的人群，如孕妇、儿童和免疫力低下的个体，这使得本文公开 的灭活Zika病毒特别适合。获得高滴度的灭活病毒是该领域的挑战。本文公开的 用于纯化灭活Zika病毒的方法不仅产生高产量，而且产生非常纯的药物物质。
本发明的进一步更详细的方面：
A1.包含灭活Zika病毒颗粒的Zika病毒疫苗，其中Zika病毒颗粒能够以至 少70％的可能性将施用Zika病毒疫苗的受试者血清转化。
A2.A1的Zika病毒疫苗，其中Zika病毒颗粒能够以至少80％、85％、90％或 95％的可能性将施用Zika病毒疫苗的受试者血清转化。
A3.A1或A2的疫苗，其中Zika病毒颗粒具有对应于由SEQ ID NO：2-13或 72的核酸序列中的任一个提供的DNA序列的RNA基因组，或与SEQ ID NO：2-13 或72中的任一个至少88％相同并且能够包裹毒性Zika病毒的变体核酸序列。
A4.A1-A3中任一项的疫苗，其中Zika病毒颗粒具有选自由SEQ ID NO：14-69 中的任一个提供的氨基酸序列中的E蛋白，或与SEQ ID NO：14-69中的任一个至 少95％相同并且能够包裹毒性Zika病毒的变体氨基酸序列。
A5.A1-A4中任一项的疫苗，其中Zika病毒通过化学灭活、热灭活、pH灭活 或UV灭活来灭活。
A6.A5的疫苗，其中化学灭活包括使Zika病毒与化学灭活剂接触以如通过斑 块测定所测量地、完全灭活Zika病毒。
A7.A6的疫苗，其中化学灭活包括使Zika病毒与甲醛接触。
A8.A7的疫苗，其中甲醛灭活包括使Zika病毒与甲醛接触2-10天。
A9.A5-A8中任一项的疫苗，其中化学活化在约+4℃或约+22℃进行。
A10.A1-A9中任一项的疫苗，其进一步包含佐剂。
A11.A10的疫苗，其中佐剂是铝盐佐剂。
A12.A11的疫苗，其中铝盐佐剂是氢氧化铝或磷酸铝盐。
A13.A10-A12中任一项的疫苗，其中疫苗包含或进一步包含含有肽和含有脱 氧肌苷的免疫刺激性寡聚脱氧核酸分子(I-ODN)的佐剂。
A14.A13的疫苗，其中肽包含序列KLKL5KLK(SEQ ID NO：71)，并且I-ODN 包含寡聚-d(IC)13(SEQ ID NO：70)。
A15.A1-A14中任一项的疫苗，其还包含一种或多种药学上可接受的赋形剂。
A16.A1-A15中任一项的疫苗，其中疫苗含有硫酸鱼精蛋白或PS的片段或分 解产物，其含量太低而不能被HPLC检测，即低于1μg/mL，尤其低于100ng/mL。
A17.A16的疫苗，其中所述硫酸鱼精蛋白或PS的片段或分解产物可以通过质 谱或其它敏感方法检测。
B1.包含A1-A15中任一项的Zika病毒疫苗的试剂盒。
B2.B1的试剂盒，还包含第二疫苗。
B3.B2的试剂盒，其中第二疫苗是西尼罗河病毒疫苗、日本脑炎病毒疫苗、 黄热病病毒疫苗、登革热病毒疫苗或基孔肯雅病毒疫苗。
C1.一种方法，其包括向有需要的受试者施用治疗有效量的第一剂量的 A1-A15中任一项的Zika病毒疫苗。
C2.C1的方法，其进一步包括施用治疗有效量的第二剂量的Zika病毒疫苗。
C3.C1或C2的方法，其中在第一剂量的Zika病毒疫苗后约7天施用第二剂 量的Zika病毒疫苗。
C4.C1或C2的方法，其中在第一剂量的Zika病毒疫苗后约14天施用第二剂 量的Zika病毒疫苗。
C5.C1或C2的方法，其中在第一剂量的Zika病毒疫苗后约28天施用第二剂 量的Zika病毒疫苗。
C6.C1-C5中任一项的方法，其中施用导致产生Zika病毒中和抗体。
D1.一种生产Zika病毒疫苗的方法，包括：
(i)在Vero细胞上传代Zika病毒，由此产生包含Zika病毒的培养物上清液；
(ii)收获(i)的培养基；
(iii)沉淀(ii)的收获培养基，从而产生Zika病毒上清液；以及
(iv)最佳地灭活(iii)的Zika病毒上清液中的Zika病毒，由此产生灭活Zika 病毒。
D2.D1的方法，其进一步包括在步骤(iii)之前浓缩(ii)的培养基。
D3.D1或D2的方法，其中(iii)的沉淀包括使(ii)的培养基与硫酸鱼精蛋 白或苯甲酸盐接触。
D4.D1-D3中任一项的方法，还包括(v)透析(iv)的灭活Zika病毒，由此 产生透析Zika病毒。
D5.D4的方法，其进一步包括(vi)过滤(v)的透析Zika病毒。
D6.D1-D5中任一项的方法，其中灭活是通过化学灭活、热灭活、pH灭活或 UV灭活进行。
D7.D6的方法，其中化学灭活包括使Zika病毒与化学灭活剂接触至少4天。
D8.D6或D7的方法，其中化学灭活剂包含甲醛。
D9.D6-D8中任一项的方法，其中化学活化在约+4℃或约+22℃下进行。
D10.D8或D9的方法，其还包括中和甲醛。
D11.D10的方法，其中用焦亚硫酸钠进行中和。
E1.A1-A15中任一项的最佳灭活Zika病毒疫苗在治疗和/或预防Zika病毒感 染中的用途。
E2.E1的用途，其中灭活Zika病毒疫苗以治疗有效量的第一剂量施用于有需 要的受试者。
E3.E2的用途，其中灭活Zika病毒疫苗以治疗有效量的第二剂量施用于受试 者。
E4.E3的用途，其中在第一剂量的Zika病毒疫苗后约7天施用第二剂量的灭 活Zika病毒疫苗。
E5.E3的用途，其中在第一剂量的Zika病毒疫苗后约14天施用第二剂量的 Zika病毒疫苗。
E6.E3的用途，其中在第一剂量的Zika病毒疫苗后约28天施用第二剂量的 Zika病毒疫苗。
E7.E1-E6中任一项的用途，其中疫苗施用导致产生Zika病毒中和抗体。
F1.用于治疗或预防Zika病毒感染的药物组合物，其中药物组合物包含 A1-A15中任一项的最佳灭活Zika病毒疫苗。
F2.F1的药物组合物，其中灭活Zika病毒疫苗以治疗有效量的第一剂量施用 于有需要的受试者。
F3.F2的用途，其中灭活Zika病毒疫苗以治疗有效量的第二剂量施用于受试 者。
F4.F3的用途，其中在第一剂量的Zika病毒疫苗后约7天施用第二剂量的灭 活Zika病毒疫苗。
F5.F3的用途，其中在第一剂量的Zika病毒疫苗后约14天施用第二剂量的 Zika病毒疫苗。
F6.F3的用途，其中在第一剂量的Zika病毒疫苗后约28天施用第二剂量的 Zika病毒疫苗。
F7.F1-F6中任一项的用途，其中疫苗施用导致产生Zika病毒中和抗体。
G1.优化蔗糖梯度离心在从纯化的感染性Zika病毒颗粒中去除硫酸鱼精蛋白 中的用途。
G2.根据G1的用途，其中所述优化蔗糖梯度离心包含含有10％+/-1％(w/w) 蔗糖溶液级分的病毒和具有不同密度的另外三层蔗糖溶液，即具有15％+/-1％ (w/w)的蔗糖的第一蔗糖溶液，具有35％+/-1％(w/w)蔗糖的第二蔗糖溶液和 具有50％+/-1％(w/w)蔗糖的第三蔗糖溶液。
G3.一种纯化感染性Zika病毒颗粒的方法，其包括以下步骤：
a)提供包含病毒颗粒和杂质的粗收获物(a)，其中所述杂质通过在细胞基质 上培养所述病毒颗粒而产生；
b)通过用包含鱼精蛋白，优选鱼精蛋白盐，更优选硫酸鱼精蛋白，甚至更优 选重组硫酸鱼精蛋白的试剂沉淀来减少粗品收获物(a)中的杂质，以获得病毒制 剂(b)；
c)通过优化蔗糖密度梯度离心进一步纯化病毒制剂(b)，其中提供优化蔗糖 梯度使得鱼精蛋白可以完全或几乎完全从病毒级分分离；并且其中通过该步骤使 鱼精蛋白浓度降低至最终药物中的鱼精蛋白浓度低于1μg/ml，优选低于0.5μg/mL， 更优选低于0.1μg/mL，最优选低于0.05μg/mL的程度。
G4.G3的方法，其中所述优化蔗糖密度梯度离心包含包含含有10％+/-1％ (w/w)蔗糖溶液级分的病毒和具有不同密度的另外三层蔗糖溶液，即具有 15％+/-1％(w/w)的蔗糖的第一蔗糖溶液，具有35％+/-1％(w/w)蔗糖的第二蔗 糖溶液和具有50％+/-1％(w/w)蔗糖的第三蔗糖溶液。
G5.G3至G4中任一项的方法，其另外包含进一步纯化步骤：
(d)填充在包含配体活化核和包含孔的无活性壳的柱中的固相基质，其中孔 的分子量截断排除病毒颗粒进入配体活化核，并且其中小于孔的分子量截断的分 子可以进入配体活化核并收集病毒颗粒。
G6.G3-G5任一项的方法，其中Zika病毒制剂(c)的残余宿主细胞DNA含 量小于10ng/mL，最终病毒制剂(c)的残余宿主细胞蛋白含量小于100ng/mL。
G7.G3-G6中任一项的方法，其中所述包含Zika病毒颗粒和杂质的粗收获物 (a)在步骤(b)之前进行一个或多个预纯化步骤。
G8.G7的方法，其中一个或多个预纯化步骤包括：
a)使用孔径等于或小于0.2μm的过滤器过滤；和/或
b)通过酶处理消化宿主细胞基因组DNA；和/或
c)使用孔径等于或大于300kDa，优选等于或大于100kDa的中空纤维膜进行 超滤/渗滤。
G9.G3-G8中任一项的方法，其中硫酸鱼精蛋白的浓度为0.5-3mg/ml，更优选 为1-2mg/ml，更优选为1.2-1.8mg/ml，更优选为1.4-1.6mg/ml，最优选为1.6mg/ml 或2mg/ml。
G10.G3-G9中任一项的方法，其中相对于粗收获物(a)中的总病毒产物，病 毒制剂(c)或任何最终病毒制剂中的感染性Zika病毒颗粒的富集在至少50％至 95％，优选至少80％的范围内。
G11.G7-G10中任一项的方法，其中G8-G11中任一项的步骤(b)之前的一 个或多个预纯化步骤使用孔径等于或小于1μm，优选0.2μm的过滤器。
G12.G3-G11中任一项的方法，其中Zika病毒制剂(c)的残余杂质小于10％。
G13.G3-G12中任一项的方法，其中Zika病毒在选自以下的细胞系中增殖： EB66细胞系、Vero细胞系、Vero-αHis细胞系、HeLa细胞系、HeLa-S3细胞系、 293细胞系、PC12细胞系、CHO细胞系、3T3细胞系、PerC6细胞系、MDSK细 胞系、鸡胚胎成纤维细胞系、鸭细胞系和二倍体禽细胞系。
G14.G13的方法，其中所述细胞系是Vero细胞系。
G15.G3-G14中任一项的方法，其中感染性Zika病毒颗粒是活病毒、活减毒 病毒、嵌合病毒、修饰的活病毒或重组活病毒的感染性病毒颗粒。
G16.G3-G15中任一项的方法，其中Zika病毒优选为亚洲谱系的菌株。
G17.G3-G16中任一项的方法，其中导致最终病毒制剂(c)或(d)的所述方 法之后是灭活步骤，其中病毒优选通过甲醛灭活。
G18.根据G3-G17中任一项的方法在制造用于免疫病毒感染的组合物中的用 途。
G19.根据G18的用途，其中所述病毒感染是由Zika病毒引起的感染。
Q1.一种纯化感染性Zika病毒颗粒的方法，其包括以下步骤：
a)提供包含病毒颗粒和杂质的粗收获物(a)，其中所述杂质通过在细胞基质 上培养所述病毒颗粒而产生；
b)通过用包含鱼精蛋白，优选鱼精蛋白盐，更优选硫酸鱼精蛋白，甚至更优 选重组硫酸鱼精蛋白的试剂沉淀来减少粗品收获物(a)中的杂质，以获得病毒制 剂(b)；
c)通过优化蔗糖密度梯度离心进一步纯化病毒制剂(b)，其中提供优化蔗糖 梯度使得鱼精蛋白可以完全或几乎完全从病毒级分分离；并且其中通过该步骤使 鱼精蛋白浓度降低至最终药物中的鱼精蛋白浓度低于1μg/ml，优选低于0.5μg/mL， 更优选低于0.1μg/mL，最优选低于0.05μg/mL的程度。
Q2.Q2的方法，其中病毒颗粒来自Zika病毒。
Q3.Q1或Q2的方法，其还包括以下步骤：
(d)填充在包含配体活化核和包含孔的无活性壳的柱中的固相基质，其中孔 的分子量截断排除病毒颗粒进入配体活化核，并且其中小于孔的分子量截断的分 子可以进入配体活化核并收集病毒颗粒。
Q4.Q1-Q3中任一项的方法，其中病毒制剂(c)的残余宿主细胞DNA小于 10ng/mL，最终病毒制剂(c)的残余宿主细胞蛋白小于100ng/mL。
Q5.Q1-Q4中任一项的方法，其中包含病毒颗粒和杂质的粗收获物(a)在步 骤(b)之前进行一个或多个预纯化步骤。
Q6.Q5的方法，其中一个或多个预纯化步骤包括：
a)使用孔径等于或小于0.2μm的过滤器过滤；和/或
b)通过酶处理消化宿主细胞基因组DNA；和/或
c)使用孔径等于或大于300kDa，优选等于或大于100kDa的中空纤维膜进行 超滤/渗滤。
Q7.Q1-Q6中任一项的方法，其中硫酸鱼精蛋白的浓度为0.5-3mg/ml，更优选 为1-2mg/ml，更优选为1.2-1.8mg/ml，更优选为1.4-1.6mg/ml，最优选为1.6mg/ml 或2mg/ml。
Q8.Q1-Q7中任一项的方法，其中相对于粗收获物(a)中的总病毒产物，病 毒制剂(c)或任何最终病毒制剂中的感染性病毒颗粒的富集在至少50％至95％， 优选至少80％的范围内。
Q9.Q5-Q8中任一项的方法，其中Q5-Q8中任一项的步骤(b)之前的一个或 多个预纯化步骤使用孔径等于或小于1μm，优选0.2μm的过滤器。
Q10.Q1-Q9中任一项的方法，其中病毒制剂(c)的残余杂质小于10％。
Q11.Q1-Q10中任一项的方法，其中病毒在选自以下的细胞系中增殖：EB66 细胞系、Vero细胞系、Vero-αHis细胞系、HeLa细胞系、HeLa-S3细胞系、293细 胞系、PC12细胞系、CHO细胞系、3T3细胞系、PerC6细胞系、MDSK细胞系、 鸡胚胎成纤维细胞系、鸭细胞系和二倍体禽细胞系。
Q12.Q11的方法，其中所述细胞系是Vero细胞系。
Q13.Q1-Q12中任一项的方法，其中感染性病毒颗粒是活病毒、减毒活病毒、 嵌合病毒、修饰的活病毒或重组活病毒的感染性Zika病毒颗粒。
Q14.Q1-Q13中任一项的方法，其中Zika病毒是亚洲谱系的Zika病毒株或其 免疫原性变体。
Q15.Q1-Q14中任一项的方法，其中导致最终病毒制剂(c)或(d)的所述方 法之后是灭活步骤，其中病毒优选通过甲醛灭活。
Q16.根据Q1-Q15中任一项的方法在制造用于免疫病毒感染的组合物中的用 途。
Q17.根据Q16的用途，其中用于免疫病毒感染的组合物是由Zika病毒引起 的感染。
Q18.包含通过Q1-Q17中任一项的方法可获得或获得的病毒颗粒的组合物， 用于治疗和/或预防感染例如Zika病毒感染。
Q19.包含在vero细胞上生长的灭活Zika病毒颗粒的Zika病毒疫苗，其中Zika 病毒颗粒能够以至少70％的可能性将施用Zika病毒疫苗的受试者血清转化并且包 含少量硫酸鱼精蛋白，优选低于检测限。
Q20.Q19的Zika病毒疫苗，其中Zika病毒颗粒能够以至少80％、85％、90％ 或95％的可能性，优选80％的可能性将施用Zika病毒疫苗的受试者血清转化。
Q21.Q19或Q20的疫苗，其中Zika病毒颗粒具有对应于由SEQ ID NO：2-13 的核酸序列中的任一个提供的DNA序列的RNA基因组，或与SEQ ID NO：2-13 中的任一个至少88％相同并且能够包裹毒性Zika病毒的变体核酸序列。
Q22.Q19、Q20和Q21中任一项的疫苗，其中Zika病毒颗粒具有选自由SEQ ID NO：14-69或72中的任一个提供的氨基酸序列的E蛋白，或与SEQ ID NO： 14-69或72中的任一个至少95％相同并且能够包裹毒性Zika病毒的变体氨基酸序 列。
Q23.Q19、Q20-Q22中任一项所述的疫苗，其中通过在Vero细胞上培养而获 得的Zika病毒通过硫酸鱼精蛋白沉淀和蔗糖梯度离心进行纯化。
Q24.Q23的疫苗，其中蔗糖梯度离心是优化蔗糖梯度离心。
Q25.Q24的疫苗，其中优化蔗糖梯度离心包含含有10％(w/w)蔗糖溶液级 分的病毒和具有不同密度的另外三层蔗糖溶液，即具有15％(w/w)的蔗糖的第一 蔗糖溶液，具有35％(w/w)蔗糖的第二蔗糖溶液和具有50％(w/w)蔗糖的第三 蔗糖溶液。
Q26.Q19、Q20-Q25中任一项的疫苗，其中Zika病毒通过化学灭活、热灭活、 pH灭活或UV灭活而灭活。
Q27.Q26的疫苗，其中化学灭活包括使Zika病毒与化学灭活剂接触的时间比 通过斑块测定所测量的完全灭活Zika病毒所需的更久。
Q28.Q27的疫苗，其中化学灭活包括使Zika病毒与甲醛接触。
Q29.Q28的疫苗，其中甲醛灭活包括使Zika病毒与甲醛接触2-10天。
Q30.Q27-Q29中任一项的疫苗，其中化学活化在约+4℃或约+22℃下进行。
Q31.Q19-Q30中任一项的疫苗，其进一步包含佐剂。
Q32.Q31的疫苗，其中佐剂是铝盐佐剂。
Q33.Q32的疫苗，其中铝盐佐剂是氢氧化铝或磷酸铝盐。
Q34.Q32的疫苗，其中基于包含Zika病毒、优选灭活Zika病毒的最终药物组 合物，铝盐佐剂是具有小于1.25ppb铜的氢氧化铝。
Q35.Q19-Q34中任一项的疫苗，其还包含一种或多种药学上可接受的赋形剂。
R1.鱼精蛋白、优选鱼精蛋白盐在分离感染性和非感染性Zika病毒颗粒、宿 主细胞蛋白和/或不确定的低分子量物质中的用途。
R2.一种纯化感染性Zika病毒颗粒的方法，其包括以下步骤：
(a)提供包含Zika病毒颗粒和杂质的粗收获物(a)，其中所述杂质通过在细 胞基质上培养所述病毒颗粒而产生；
(b)通过用包含鱼精蛋白，优选鱼精蛋白盐，更优选硫酸鱼精蛋白，甚至更 优选重组鱼精蛋白硫酸盐的试剂沉淀来减少粗收获物(a)中的杂质，以获得病毒 制剂(b)，其中相对于粗收获物(a)中的总病毒产物，病毒制剂(b)中的感染 性病毒颗粒的富集在至少50％至95％，优选至少80％的范围内。
R3.R1的用途或R2的方法，其中病毒颗粒来自Zika病毒。
R4.一种纯化感染性Zika病毒颗粒的方法，其包括以下步骤：
(a)提供包含Zika病毒颗粒和杂质的粗收获物(a)，其中所述杂质通过在细 胞基质上培养所述病毒颗粒而产生；
(b)通过用包含鱼精蛋白，优选鱼精蛋白盐，更优选硫酸鱼精蛋白，甚至更 优选重组鱼精蛋白硫酸盐的试剂沉淀来减少粗品收获物(a)中的杂质，以获得病 毒制剂(b)；
(c)通过一种或多种尺寸排阻方法进一步纯化病毒制剂(b)，例如(i)蔗糖 密度梯度离心，(ii)填充在包含配体活化核和包含孔的无活性壳的柱中的固相基 质，其中孔的分子量截断排除病毒颗粒进入配体活化核，并且其中小于孔的分子 量截断的分子可以进入配体活化核并收集病毒颗粒，和/或(iii)尺寸排阻色谱以 获得包含感染性病毒颗粒的病毒制剂(c)，其中病毒制剂(c)的残余宿主细胞DNA 低于100ng/mL，并且最终病毒制剂(c)的残余宿主细胞蛋白和感染性病毒颗粒的 残余聚集体小于1μg/mL。
R5.R4的方法，其中病毒制剂(c)的残余宿主细胞DNA低于10ng/mL，最 终病毒制剂(c)的残余宿主细胞蛋白低于100ng/mL。
R6.R2-R5中任一项的方法，其中包含病毒颗粒和杂质的粗收获物(a)在步 骤(b)之前进行一个或多个预纯化步骤。
R7.R6的方法，其中一个或多个预纯化步骤包括：
(a)使用孔径等于或小于0.2μm的过滤器过滤；和/或
(b)通过酶处理消化宿主细胞基因组DNA；和/或
(c)使用孔径等于或大于300kDa，优选等于或大于100kDa的中空纤维膜进 行超滤/渗滤。
R8.R2-R7中任一项的方法，其中硫酸鱼精蛋白的浓度为0.5-3mg/ml，更优选 为1-2mg/ml，更优选为1.2-1.8mg/ml，更优选为1.4-1.6mg/ml，最优选为1.6mg/ml 或2mg/ml。
R9.R2-R8中任一项的方法，其中相对于粗收获物(a)中的总病毒产物，病 毒制剂(c)或任何最终病毒制剂中的感染性病毒颗粒的富集在至少50％至95％， 优选至少80％的范围内。
R10.R6-R9中任一项的方法，其中R6-R9中任一项的步骤(b)之前的一个或 多个预纯化步骤使用孔径等于或小于1μm，优选0.2μm的过滤器。
R11.R2-R10中任一项的方法，其中病毒制剂(c)的残余杂质小于10％。
R12.R2-R11中任一项的方法，其中病毒在选自以下的细胞系中增殖：EB66 细胞系、Vero细胞系、Vero-αHis细胞系、HeLa细胞系、HeLa-S3细胞系、293细 胞系、PC12细胞系、CHO细胞系、3T3细胞系、PerC6细胞系、MDSK细胞系、 鸡胚胎成纤维细胞系、鸭细胞系和二倍体禽细胞系。
R13.R12的方法，其中所述细胞系是Vero细胞系。
R14.R2-R13中任一项的方法，其中Zika病毒是活病毒、减毒活病毒、嵌合病 毒、修饰的活病毒或重组活病毒。
R15.R2-R14中任一项的方法，其中Zika病毒是亚洲谱系的Zika病毒株或其 免疫原性变体。
R16.R2-R15中任一项的方法，其中导致最终病毒制剂(c)的所述方法之后 是灭活步骤，其中病毒优选通过甲醛灭活。
R17.根据R1-R16中任一项的方法在制造用于免疫病毒感染的组合物中的用 途。
R18.根据R17的用途，其中用于免疫病毒感染的组合物是由Zika病毒引起的 感染。
R19.包含通过R2-R16中任一项的方法可获得或获得的组合物，用于治疗和/ 或预防感染的病毒颗粒。
序列表
<110> 瓦尔尼瓦奥地利有限责任公司
<120> Zika病毒疫苗
<130> 620563CG-680934
<160> 117
<170> PatentIn version 3.5
<210> 1
<211> 32
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 1
Pro Arg Arg Arg Arg Ser Ser Ser Arg Pro Val Arg Arg Arg Arg Arg
1 5 10 15
Pro Arg Val Ser Arg Arg Arg Arg Arg Arg Gly Gly Arg Arg Arg Arg
20 25 30
<210> 2
<211> 10676
<212> DNA
<213> Zika病毒
<400> 2
gttgttactg ttgctgactc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60
gtatcaacag gttttatttg gatttggaaa cgagagtttc tggtcatgaa aaacccaaaa 120
aagaaatccg gaggattccg gattgtcaat atgctaaaac gcggagtagc ccgtgtgagc 180
ccctttgggg gcttgaagag gctgccagcc ggacttctgc tgggtcatgg gcccatcagg 240
atggtcttgg caattctagc ctttttgaga ttcacggcaa tcaagccatc actgggtctc 300
atcaatagat ggggttcagt ggggaaaaaa gaggctatgg aaataataaa gaagttcaag 360
aaagatctgg ctgccatgct gagaataatc aatgctagga aggagaagaa gagacggggc 420
gcagatacta gtgtcggaat tgttggcctc ctgctgacca cagctatggc agcggaggtc 480
actagacgtg ggagtgcata ctatatgtac ttggacagaa acgatgctgg ggaggccata 540
tcttttccaa ccacattggg gatgaataag tgttatatac agatcatgga tcttggacac 600
atgtgtgatg ccaccatgag ctatgaatgc cctatgctgg atgagggggt ggaaccagat 660
gacgtcgatt gttggtgcaa cacgacgtca acttgggttg tgtacggaac ctgccatcac 720
aaaaaaggtg aagcacggag atctagaaga gctgtgacgc tcccctccca ttccactagg 780
aagctgcaaa cgcggtcgca aacctggttg gaatcaagag aatacacaaa gcacttgatt 840
agagtcgaaa attggatatt caggaaccct ggcttcgcgt tagcagcagc tgccatcgct 900
tggcttttgg gaagctcaac gagccaaaaa gtcatatact tggtcatgat actgctgatt 960
gccccggcat acagcatcag gtgcatagga gtcagcaata gggactttgt ggaaggtatg 1020
tcaggtggga cttgggttga tattgtcttg gaacatggag gttgtgtcac cgtaatggca 1080
caggacaaac cgactgtcga catagagctg gttacaacaa cagtcagcaa catggcggag 1140
gtaagatcct actgctatga ggcatcaata tcagacatgg cttcggacag ccgctgccca 1200
acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtctg caaaagaacg 1260
ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag tctggtgaca 1320
tgcgctaagt ttgcatgctc caagaaaatg accgggaaga gcatccagcc agagaatctg 1380
gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgat cgttaatgac 1440
acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa ttcaccaaga 1500
gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc gaggacaggc 1560
cttgactttt cagatttgta ttacttgact atgaataaca agcactggtt ggttcacaag 1620
gagtggttcc acgacattcc attaccttgg cacgctgggg cagacaccgg aactccacac 1680
tggaacaaca aagaagcact ggtagagttc aaggacgcac atgccaaaag gcaaactgtc 1740
gtggttctag ggagtcaaga aggagcagtt cacacggccc ttgctggagc tctggaggct 1800
gagatggatg gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg cctgaaaatg 1860
gataaactta gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt cacattcacc 1920
aagatcccgg ctgaaacact gcacgggaca gtcacagtgg aggtacagta cgcagggaca 1980
gatggacctt gcaaggttcc agctcagatg gcggtggaca tgcaaactct gaccccagtt 2040
gggaggttga taaccgctaa ccccgtaatc actgaaagca ctgagaactc taagatgatg 2100
ctggaacttg atccaccatt tggggactct tacattgtca taggagtcgg ggagaagaag 2160
atcacccacc actggcacag gagtggcagc accattggaa aagcatttga agccactgtg 2220
agaggtgcca agagaatggc agtcttggga gacacagcct gggactttgg atcagttgga 2280
ggcgctctca actcattggg caagggcatc catcaaattt ttggagcagc tttcaaatca 2340
ttgtttggag gaatgtcctg gttctcacaa attctcattg gaacgttgct gatgtggttg 2400
ggtctgaaca caaagaatgg atctatttcc cttatgtgct tggccttagg gggagtgttg 2460
atcttcttat ccacagccgt ctctgctgat gtggggtgct cggtggactt ctcaaagaag 2520
gagacgagat gcggtacagg ggtgttcgtc tataacgacg ttgaagcctg gagggacagg 2580
tacaagtacc atcctgactc cccccgtaga ttggcagcag cagtcaagca agcctgggaa 2640
gatggtatct gcgggatctc ctctgtttca agaatggaaa acatcatgtg gagatcagta 2700
gaaggggagc tcaacgcaat cctggaagag aatggagttc aactgacggt cgttgtggga 2760
tctgtaaaaa accccatgtg gagaggtcca cagagattgc ccgtgcctgt gaacgagctg 2820
ccccacggct ggaaggcttg ggggaaatcg cacttcgtca gagcagcaaa gacaaataac 2880
agctttgtcg tggatggtga cacactgaag gaatgcccac tcaaacatag agcatggaac 2940
agctttcttg tggaggatca tgggttcggg gtatttcaca ctagtgtctg gctcaaggtt 3000
agagaagatt attcattaga gtgtgatcca gccgttattg gaacagctgt taagggaaag 3060
gaggctgtac acagtgatct aggctactgg attgagagtg agaagaatga cacatggagg 3120
ctgaagaggg cccatctgat cgagatgaaa acatgtgaat ggccaaagtc ccacacattg 3180
tggacagatg gaatagaaga gagtgatctg atcataccca agtctttagc tgggccactc 3240
agccatcaca ataccagaga gggctacagg acccaaatga aagggccatg gcacagtgaa 3300
gagcttgaaa ttcggtttga ggaatgccca ggcactaagg tccacgtgga ggaaacatgt 3360
ggaacaagag gaccatctct gagatcaacc actgcaagcg gaagggtgat cgaggaatgg 3420
tgctgcaggg agtgcacaat gcccccactg tcgttccggg ctaaagatgg ctgttggtat 3480
ggaatggaga taaggcccag gaaagaacca gaaagcaact tagtaaggtc aatggtgact 3540
gcaggatcaa ctgatcacat ggatcacttc tcccttggag tgcttgtgat tctgctcatg 3600
gtgcaggaag ggctgaagaa gagaatgacc acaaagatca tcataagcac atcaatggca 3660
gtgctggtag ctatgatcct gggaggattt tcaatgagtg acctggctaa gcttgcaatt 3720
ttgatgggtg ccaccttcgc ggaaatgaac actggaggag atgtagctca tctggcgctg 3780
atagcggcat tcaaagtcag accagcgttg ctggtatctt tcatcttcag agctaattgg 3840
acaccccgtg aaagcatgct gctggccttg gcctcgtgtc ttttgcaaac tgcgatctcc 3900
gccttggaag gcgacctgat ggttctcatc aatggttttg ctttggcctg gttggcaata 3960
cgagcgatgg ttgttccacg cactgataac atcaccttgg caatcctggc tgctctgaca 4020
ccactggccc ggggcacact gcttgtggcg tggagagcag gccttgctac ttgcgggggg 4080
tttatgctcc tctctctgaa gggaaaaggc agtgtgaaga agaacttacc atttgtcatg 4140
gccctgggac taaccgctgt gaggctggtc gaccccatca acgtggtggg gctgctgttg 4200
ctcacaagga gtgggaagcg gagctggccc cctagcgaag tactcacagc tgttggcctg 4260
atatgcgcat tggctggagg gttcgccaag gcagatatag agatggctgg gcccatggcc 4320
gcggtcggtc tgctaattgt cagttacgtg gtctcaggaa agagtgtgga catgtacatt 4380
gaaagagcag gtgacatcac atgggaaaaa gatgcggaag tcactggaaa cagtccccgg 4440
ctcgatgtgg cgctagatga gagtggtgat ttctccctgg tggaggatga cggtcccccc 4500
atgagagaga tcatactcaa ggtggtcctg atgaccatct gtggcatgaa cccaatagcc 4560
ataccctttg cagctggagc gtggtacgta tacgtgaaga ctggaaaaag gagtggtgct 4620
ctatgggatg tgcctgctcc caaggaagta aaaaaggggg agaccacaga tggagtgtac 4680
agagtaatga ctcgtagact gctaggttca acacaagttg gagtgggagt tatgcaagag 4740
ggggtctttc acactatgtg gcacgtcaca aaaggatccg cgctgagaag cggtgaaggg 4800
agacttgatc catactgggg agatgtcaag caggatctgg tgtcatactg tggtccatgg 4860
aagctagatg ccgcctggga cgggcacagc gaggtgcagc tcttggccgt gccccccgga 4920
gagagagcga ggaacatcca gactctgccc ggaatattta agacaaagga tggggacatt 4980
ggagcggttg cgctggatta cccagcagga acttcaggat ctccaatcct agacaagtgt 5040
gggagagtga taggacttta tggcaatggg gtcgtgatca aaaatgggag ttatgttagt 5100
gccatcaccc aagggaggag ggaggaagag actcctgttg agtgcttcga gccttcgatg 5160
ctgaagaaga agcagctaac tgtcttagac ttgcatcctg gagctgggaa aaccaggaga 5220
gttcttcctg aaatagtccg tgaagccata aaaacaagac tccgtactgt gatcttagct 5280
ccaaccaggg ttgtcgctgc tgaaatggag gaagccctta gagggcttcc agtgcgttat 5340
atgacaacag cagtcaatgt cacccactct ggaacagaaa tcgtcgactt aatgtgccat 5400
gccaccttca cttcacgtct actacagcca atcagagtcc ccaactataa tctgtatatt 5460
atggatgagg cccacttcac agatccctca agtatagcag caagaggata catttcaaca 5520
agggttgaga tgggcgaggc ggctgccatc ttcatgaccg ccacgccacc aggaacccgt 5580
gacgcatttc cggactccaa ctcaccaatt atggacaccg aagtggaagt cccagagaga 5640
gcctggagct caggctttga ttgggtgacg gattattctg gaaaaacagt ttggtttgtt 5700
ccaagcgtga ggaacggcaa tgagatcgca gcttgtctga caaaggctgg aaaacgggtc 5760
atacagctca gcagaaagac ttttgagaca gagttccaga aaacaaaaca tcaagagtgg 5820
gactttgtcg tgacaactga catttcagag atgggcgcca actttaaagc tgaccgtgtc 5880
atagattcca ggagatgcct aaagccggtc atacttgatg gcgagagagt cattctggct 5940
ggacccatgc ctgtcacaca tgccagcgct gcccagagga gggggcgcat aggcaggaat 6000
cccaacaaac ctggagatga gtatctgtat ggaggtgggt gcgcagagac tgacgaagac 6060
catgcacact ggcttgaagc aagaatgctc cttgacaata tttacctcca agatggcctc 6120
atagcctcgc tctatcgacc tgaggccgac aaagtagcag ccattgaggg agagttcaag 6180
cttaggacgg agcaaaggaa gacctttgtg gaactcatga aaagaggaga tcttcctgtt 6240
tggctggcct atcaggttgc atctgccgga ataacctaca cagatagaag atggtgcttt 6300
gatggcacga ccaacaacac cataatggaa gacagtgtgc cggcagaggt gtggaccaga 6360
cacggagaga aaagagtgct caaaccgagg tggatggacg ccagagtttg ttcagatcat 6420
gcggccctga agtcattcaa ggagtttgcc gctgggaaaa gaggagcggc ttttggagtg 6480
atggaagccc tgggaacact gccaggacac atgacagaga gattccagga agccattgac 6540
aacctcgctg tgctcatgcg ggcagagact ggaagcaggc cttacaaagc cgcggcggcc 6600
caattgccgg agaccctaga gaccattatg cttttggggt tgctgggaac agtctcgctg 6660
ggaatctttt tcgtcttgat gaggaacaag ggcataggga agatgggctt tggaatggtg 6720
actcttgggg ccagcgcatg gctcatgtgg ctctcggaaa ttgagccagc cagaattgca 6780
tgtgtcctca ttgttgtgtt cctattgctg gtggtgctca tacctgagcc agaaaagcaa 6840
agatctcccc aggacaacca aatggcaatc atcatcatgg tagcagtagg tcttctgggc 6900
ttgattaccg ccaatgaact cggatggttg gagagaacaa agagtgacct aagccatcta 6960
atgggaagga gagaggaggg ggcaaccatg ggattctcaa tggacattga cctgcggcca 7020
gcctcagctt gggccatcta tgctgccttg acaactttca ttaccccagc cgtccaacat 7080
gcagtgacca cttcatacaa caactactcc ttaatggcga tggccacgca agctggagtg 7140
ttgtttggta tgggcaaagg gatgccattc tacgcatggg actttggagt cccgctgcta 7200
atgataggtt gctactcaca attaacgccc ctgaccctaa tagtggccat cattttgctc 7260
gtggcgcact acatgtactt gatcccaggg ctgcaggcag cagctgcgcg tgctgcccag 7320
aagagaacgg cagctggcat catgaagaac cctgttgtgg atggaatagt ggtgactgac 7380
attgacacaa tgacaattga cccccaagtg gagaaaaaga tgggacaggt gctactcatg 7440
gcagtagccg tctccagcgc catactgtcg cggaccgcct gggggtgggg ggaggctggg 7500
gccctgatca cagccgcaac ttccactttg tgggaaggct ctccgaacaa gtactggaac 7560
tcctctacag ccacttcact gtgtaacatt tttaggggaa gttacttggc tggagcttct 7620
ctaatctaca cagtaacaag aaacgctggc ttggtcaaga gacgtggggg tggaacagga 7680
gagaccctgg gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac 7740
tcctacaaaa agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag 7800
gacggtgtgg caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg 7860
gtggagcggg gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg 7920
ggctggagtt actacgccgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa 7980
ggaggccctg gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt 8040
cttaagagtg gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt 8100
gacataggtg agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc 8160
tccatggtgg gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc 8220
ccatacacca gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga 8280
ctggtcagag tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg 8340
aaaagcaaca ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac 8400
gggcctagga ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct 8460
gtggtaagct gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc 8520
cgcagtgagc acgcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct 8580
taccatggaa gctatgaggc ccccacacaa gggtcagcgt cctctctaat aaacggggtt 8640
gtcaggctcc tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc 8700
gacaccacac cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca 8760
gacccccaag aaggtactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag 8820
ctaggcaaac acaaacggcc acgagtctgt accaaagaag agttcatcaa caaggttcgt 8880
agcaatgcag cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa 8940
gctgtgaacg atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga 9000
ggagagtgcc agagttgtgt gtacaacatg atgggaaaaa gagaaaagaa acaaggggaa 9060
tttggaaagg ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta 9120
gagttcgaag cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga 9180
ggtggtgttg aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgc 9240
ataccaggag gaaggatgta tgcagatgac actgctggct gggacacccg catcagcagg 9300
tttgatctgg agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg 9360
gcattggcca taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct 9420
gaaaaaggga aaacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa 9480
gttgtcactt acgctcttaa cacatttacc aacctagtgg tgcaactcat tcggaatatg 9540
gaggctgagg aagtcctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg 9600
accaactggt tgcagagcaa cggatgggat aggctcaaac gaatggcagt cagtggagat 9660
gattgcgttg tgaagccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat 9720
atgggaaaag ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg 9780
gaagaagttc cgttttgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc 9840
attgtggttc cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg 9900
gcgggatgga gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag 9960
ctcctttatt tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg 10020
ccagttgact gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg 10080
atgaccactg aagacatgct tgtggtgtgg aacagagtgt ggattgagga gaacgaccac 10140
atggaagaca agaccccagt tacgaaatgg acagacattc cctatttggg aaaaagggaa 10200
gacttgtggt gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt 10260
aaaaacacag tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac 10320
ctatccaccc aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtaagca 10380
ccaatcttaa tgttgtcagg cctgctagtc agccacagct tggggaaagc tgtgcagcct 10440
gtgacccccc caggagaagc tgggaaacca agcctatagt caggccgaga acgccatggc 10500
acggaagaag ccatgctgcc tgtgagcccc tcagaggaca ctgagtcaaa aaaccccacg 10560
cgcttggagg cgcaggatgg gaaaagaagg tggcgacctt ccccaccctt caatctgggg 10620
cctgaactgg agatcagctg tggatctcca gaagagggac tagtggttag aggaga 10676
<210> 3
<211> 10793
<212> DNA
<213> Zika病毒
<400> 3
ccaatctgtg aatcagactg cgacagttcg agtttgaagc gaaagctagc aacagtatca 60
acaggtttta ttttggattt ggaaacgaga gtttctggtc atgaaaaacc caaaaaagaa 120
atccggagga ttccggattg tcaatatgct aaaacgcgga gtagcccgtg tgagcccctt 180
tgggggcttg aagaggctgc cagccggact tctgctgggt catgggccca tcaggatggt 240
cttggcgatt ctagcctttt tgagattcac ggcaatcaag ccatcactgg gtctcatcaa 300
tagatggggt tcagtgggga aaaaagaggc tatggaaata ataaagaagt tcaagaaaga 360
tctggctgcc atgctgagaa taatcaatgc caggaaggag aagaagagac gaggcgcaga 420
tactagtgtc ggaatcgttg gcctcctgct gaccacagct atggcagcgg aggtcactag 480
acgtgggagt gcatactata tgtacttgga cagaaacgat gctggggagg ccatatcttt 540
tccaaccaca ttggggatga ataagtgtta tatacagatc atggatcttg gacacatgtg 600
tgatgccacc atgagctatg aatgccctat gctggatgag ggggtggaac cagatgacgt 660
cgattgttgg tgcaacacga cgtcaacttg ggttgtgtac ggaacctgcc atcacaaaaa 720
aggtgaagca cggagatcta gaagagctgt gacgctcccc tcccattcca ctaggaagct 780
gcaaacgcgg tcgcaaacct ggttggaatc aagagaatac acaaagcact tgattagagt 840
cgaaaattgg atattcagga accctggctt cgcgttagca gcagctgcca tcgcttggct 900
tttgggaagc tcaacgagcc aaaaagtcat atacttggtc atgatactgc tgattgcccc 960
ggcatacagc atcaggtgca taggagtcag caatagggac tttgtggaag gtatgtcagg 1020
tgggacttgg gttgatgttg tcttggaaca tgggggttgt gtcaccgtaa tggcacagga 1080
caaaccgact gtcgacatag agctggttac aacaacagtc agcaacatgg cggaggtaag 1140
atcctactgc tatgaggcat caatatcaga catggcttcg gacagccgct gcccaacaca 1200
aggtgaagcc taccttgaca agcaatcaga cactcaatat gtctgcaaaa gaacgttagt 1260
ggacagaggc tggggaaatg gatgtggact ttttggcaaa gggagcctgg tgacatgcgc 1320
taagtttgca tgctccaaga aaatgaccgg gaagagcatc cagccagaga atctggagta 1380
ccggataatg ctgtcagttc atggctccca gcacagtggg atgatcgtta atgacacagg 1440
acatgaaact gatgagaata gagcgaaggt tgagataacg cccaattcac caagagccga 1500
agccaccctg gggggttttg gaagcttagg acttgattgt gaaccgagga caggccttga 1560
cttttcagat ttgtattact tgactatgaa taacaagcac tggttggttc acaaggagtg 1620
gttccacgac attccattac cttggcacgc tggggcagac accggaactc cacactggaa 1680
caacaaagaa gcactggtag agttcaagga cgcacatgcc aaaaggcaaa ctgtcgtggt 1740
tctagggact caagaaggag cagttcacac ggcccttgct ggagctctgg aggctgagat 1800
ggatggtgca aagggaaggc tgtcctctgg ccacttgaaa tgtcgcctga aaatggataa 1860
acttagattg aagggcgtgt catactcctt gtgtaccgca gcgttcacat tcaccaagat 1920
cccggctgaa acactgcacg ggacagtcac agtggaggta cagtacgcag ggacagatgg 1980
accttgcaag gttccagctc agatggcggt ggacatgcaa actctgaccc cagttgggag 2040
gttgataacc gctaaccccg taatcactga aagcactgag aactctaaga tgatgctgga 2100
acttgatcca ccatttgggg actcttacat tgtcatagga gtcggggaga agaagatcac 2160
ccaccactgg cacaggagtg gcagcaccat tggaaaagca tttgaagcca ctgtgagagg 2220
tgccaagaga atggcagtct tgggagacac agcctgggac tttggatcag ttggaggcgc 2280
tctcaactca ttgggcaagg gcatccatca aatttttgga gcagctttca aatcattgtt 2340
tggaggaatg tcctggttct cacaaattct cattggaacg ttgctgatgt ggttgggtct 2400
gaacacaaag aatggatcta tttcccttat gtgcttggcc ttagggggag tgttgatctt 2460
cttatccaca gccgtctctg ctgatgtggg gtgctcggtg gacttctcaa agaaggagac 2520
gagatgtggt acaggggtgt tcgtctataa cgacgttgaa gcctggaggg acaggtacaa 2580
gtaccatcct gactctcccc gtagattggc agcagcagtc aagcaagcct gggaagatgg 2640
tatctgcggg atctcctctg tttcaagaat ggaaaacatc atgtggagat cagtagaagg 2700
ggagcttaac gcaatcctgg aagagaatgg agttcaactg acggtcgttg tgggatctgt 2760
aaaaaacccc atgtggagag gtccacagag attgcccgtg cctgtgaacg agctgcccca 2820
cggctggaag gcttggggga aatcgtactt cgtcagagca gcaaagacaa ataacagctt 2880
tgtcgtggat ggtgacacac tgaaggaatg cccactcaaa catagagcat ggaacagctt 2940
tcttgtggag gatcatgggt tcggggtatt tcacactagt gtctggctca aggttagaga 3000
agattattca ttagagtgtg atccagccgt tattggaaca gctgttaagg gaaaggaggc 3060
tgtacacagt gatctaggct actggattga gagtgagaag aatgacacat ggaggctgaa 3120
gagggcccat ctgatcgaga tgaaaacatg tgaatggcca aagtcccaca cattgtggac 3180
agatggaata gaagagagtg atctgatcat acccaagtct ttagctgggc cactcagcca 3240
tcacaatacc agagagggct acaggaccca aatgaaaggg ccatggcaca gtgaagagct 3300
tgaaattcgg tttgaggaat gcccaggcac taaggtccac gtggaggaaa catgtggaac 3360
aagaggacca tctctgagat caaccactgc aagcggaagg gtgatcgagg aatggtgctg 3420
cagggagtgc acaatgcccc cactgtcgtt ccgggctaaa gatggctgtt ggtatggaat 3480
ggagataagg cccaggaaag aaccagaaag caacttagta aggtcaatgg tgactgcagg 3540
atcaactgat cacatggatc acttctccct tggagtgctt gtgattctgc tcatggtgca 3600
ggaagggctg aagaagagaa tgaccacaaa gatcatcata agcacatcaa tggcagtgct 3660
ggtagctatg atcctgggag gattttcaat gagtgacctg gctaagcttg caattttgat 3720
gggtgccacc ttcgcggaaa tgaacactgg aggagatgta gctcatctgg cgctgatagc 3780
ggcattcaaa gtcagaccag cgttgctggt atctttcatc ttcagagcta attggacacc 3840
ccgtgaaagc atgctgctgg ccttggcctc gtgttttttg caaactgcga tctccgcctt 3900
ggaaggcgac ctgatggttc tcatcaatgg ttttgctttg gcctggttgg caatacgagc 3960
gatggttgtt ccacgcactg acaacatcac cttggcaatc ctggctgctc tgacaccact 4020
ggcccggggc acactgcttg tggcgtggag agcaggcctt gctacttgcg gggggtttat 4080
gctcctctct ctgaagggaa aaggcagtgt gaagaagaac ttaccatttg tcatggccct 4140
gggactaacc gctgtgaggc tggtcgaccc catcaacgtg gtgggactgc tgttgctcac 4200
aaggagtggg aagcggagct ggccccctag cgaagtactc acagctgttg gcctgatatg 4260
cgcattggct ggagggttcg ccaaggcaga tatagagatg gctgggccca tggccgcggt 4320
cggtctgcta attgtcagtt acgtggtctc aggaaagagt gtggacatgt acattgaaag 4380
agcaggtgac atcacatggg aaaaagatgc ggaagtcact ggaaacagtc cccggctcga 4440
tgtggcgcta gatgagagtg gtgacttctc cctggtggag gatgacggtc cccccatgag 4500
agagatcata ctcaaggtgg tcctgatgac catctgtggc atgaacccaa tagccatacc 4560
ctttgcagct ggagcgtggt acgtatacgt gaagactgga aaaaggagtg gtgctctatg 4620
ggatgtgcct gctcccaagg aagtaaaaaa gggggagacc acagatggag tgtacagagt 4680
aatgactcgt agactgctag gttcaacaca agttggagtg ggagttatgc aagagggggt 4740
ctttcacact atgtggcacg tcacaaaagg atccgcgctg agaagcggtg aagggagact 4800
tgatccatac tggggagatg tcaagcagga tctggtgtca tactgtggtc catggaagct 4860
agatgccgcc tgggacgggc acagcgaggt gcagctcttg gccgtgcccc ccggagagag 4920
agcgaggaac atccagactc tgcccggaat atttaagaca aaggatgggg acattggagc 4980
ggttgcgctg gattacccag caggaacttc aggatctcca atcctagaca agtgtgggag 5040
agtgatagga ctttatggca atggggtcgt gataaaaaat gggagttatg ttagtgccat 5100
cacccaaggg aggagggagg aagagactcc tgttgagtgc ttcgagcctt cgatgctgaa 5160
gaagaagcag ctaactgtct tagacttgca tcctggagct gggaaaacca ggagagttct 5220
tcctgaaata gtccgtgaag ccataaaaac aagactccgt actgtgatct tagctccaac 5280
cagggttgtc gctgctgaaa tggaggaagc ccttagaggg cttccagtgc gttatatgac 5340
aacagcagtc aatgtcaccc actctggaac agaaatcgtc gacttaatgt gccatgccac 5400
cttcacttca cgtctactac agccaatcag agtccccaac tataatctgt atattatgga 5460
tgaggcccac ttcacagatc cctcaagcat agcagcaaga ggatacattt caacaagggt 5520
tgagatgggc gaggcggctg ccatcttcat gaccgccacg ccaccaggaa cccgtgacgc 5580
atttccggac tccaactcac caattatgga caccgaagtg gaagtcccag agagagcctg 5640
gagctcaggc tttgattggg tgacggatca ttctggaaaa acagtttggt ttgttccaag 5700
cgtgaggaac ggcaatgaga tcgcagcttg tctgacaaag gctggaaaac gggtcataca 5760
gctcagcaga aagacttttg agacagagtt ccagaaaaca aaacatcaag agtgggactt 5820
tgtcgtgaca actgacattt cagagatggg cgccaacttt aaagctgacc gtgtcataga 5880
ttccaggaga tgcctaaagc cggtcatact tgatggcgag agagtcattc tggctggacc 5940
catgcctgtc acacatgcca gcgctgccca gaggaggggg cgcataggca ggaatcccaa 6000
caaacctgga gatgagtacc tgtatggagg tgggtgcgca gagactgacg aagaccatgc 6060
acactggctt gaagcaagaa tgctccttga caatatttac ctccaagatg gcctcatagc 6120
ctcgctctat cgacctgagg ccgacaaagt agcagccatt gagggagagt tcaagcttag 6180
gacggagcaa aggaagacct ttgtggaact catgaaaaga ggagatcttc ctgtttggct 6240
ggcctatcag gttgcatctg ccggaataac ctacacagat agaagatggt gctttgatgg 6300
cacgaccaac aacaccataa tggaagacag tgtgccggca gaggtgtgga ccagacacgg 6360
agagaaaaga gtgctcaaac cgaggtggat ggacgccaga gtttgttcag atcatgcggc 6420
cctgaagtca ttcaaggagt ttgccgctgg gaaaagagga gcggcttttg gagtgatgga 6480
agccctggga acactgccag gacacatgac agagagattc caggaagcca ttgacaacct 6540
cgctgtgctc atgcgggcag agactggaag caggccttac aaagccgcgg cggcccaatt 6600
gccggagacc ctagagacca ttatgctttt ggggttgctg ggaacagtct cgctgggaat 6660
ctttttcgtc ttgatgagga acaagggcat agggaagatg ggctttggaa tggtgactct 6720
tggggccagc gcatggctca tgtggctctc ggaaattgag ccagccagaa ttgcatgtgt 6780
cctcattgtt gtgttcctat tgctggtggt gctcatacct gagccagaaa agcaaagatc 6840
tccccaggac aaccaaatgg caatcatcat catggtagca gtaggtcttc tgggcttgat 6900
taccgccaat gaactcggat ggttggagag aacaaagagt gacctaagcc atctaatggg 6960
aaggagagag gagggggcaa ccataggatt ctcaatggac attgacctgc ggccagcctc 7020
agcttgggcc atctatgctg ccttgacaac tttcattacc ccagccgtcc aacatgcagt 7080
gaccacttca tacaacaact actccttaat ggcgatggcc acgcaagctg gagtgttgtt 7140
tggtatgggc aaagggatgc cattctacgc atgggacttt ggagtcccgc tgctaatgat 7200
aggttgctac tcacaattaa cacccctgac cctaatagtg gccatcattt tgctcgtggc 7260
gcactacatg tacttgatcc cagggctgca ggcagcagct gcgcgtgctg cccagaagag 7320
aacggcagct ggcatcatga agaaccctgt tgtggatgga atagtggtga ctgacattga 7380
cacaatgaca attgaccccc aagtggagaa aaagatggga caggtgctac tcatagcagt 7440
agccgtctcc agcgccatac tgtcgcggac cgcctggggg tggggggagg ctggggccct 7500
gatcacagcc gcaacttcca ctttgtggga aggctctccg aacaagtact ggaactcctc 7560
tacagccact tcactgtgta acatttttag gggaagttac ttggctggag cttctctaat 7620
ctacacagta acaagaaacg ctggcttggt caagagacgt gggggtggaa caggagagac 7680
cctgggagag aaatggaagg cccgcttgaa ccagatgtcg gccctggagt tctactccta 7740
caaaaagtca ggcatcaccg aggtgtgcag agaagaggcc cgccgcgccc tcaaggacgg 7800
tgtggcaacg ggaggccatg ctgtgtcccg aggaagtgca aagctgagat ggttggtgga 7860
gcggggatac ctgcagccct atggaaaggt cattgatctt ggatgtggca gagggggctg 7920
gagttactac gccgccacca tccgcaaagt tcaagaagtg aaaggataca caaaaggagg 7980
ccctggtcat gaagaacccg tgttggtgca aagctatggg tggaacatag tccgtcttaa 8040
gagtggggtg gacgtctttc atatggcggc tgagccgtgt gacacgttgc tgtgtgacat 8100
aggtgagtca tcatctagtc ctgaagtgga agaagcacgg acgctcagag tcctctccat 8160
ggtgggggat tggcttgaaa aaagaccagg agccttttgc ataaaagtgt tgtgcccata 8220
caccagcact atgatggaaa ccctggagcg actgcagcgt aggtatgggg gaggactggt 8280
cagagtgcca ctctcccgca actctacaca tgagatgtac tgggtctctg gagcgaaaag 8340
caacaccata aaaagtgtgt ccaccacgag ccagctcctc ttggggcgca tggacgggcc 8400
taggaggcca gtgaaatatg aggaggatgt gaatctcggc tctggcacgc gggctgtggt 8460
aagctgcgct gaagctccca acatgaagat cattggtaac cgcattgaaa ggatccgcag 8520
tgagcacgcg gaaacgtggt tctttgacga aaaccaccca tataggacat gggcttacca 8580
tggaagctat gtggccccca cacaagggtc agcgtcctct ctaataaacg gggttgtcag 8640
gctcctgtca aaaccctggg atgtggtgac tggagtcaca ggaatagcca tgaccgacac 8700
cacaccgtat ggtcagcaaa gagttttcaa ggaaaaagtg gacactaggg tgccagaccc 8760
ccaagaaggc actcgtcagg ttatgagcat ggtctcttcc tggttgtgga aagagctagg 8820
caaacacaaa cgaccacgag tctgtaccaa agaagagttc atcaacaagg ttcgtagcaa 8880
tgcagcatta ggggcaatat ttgaagagga aaaagagtgg aagactgcag tggaagctgt 8940
gaacgatcca aggttctggg ctctagtgga caaggaaaga gagcaccacc tgagaggaga 9000
gtgccagagt tgtgtgtaca acatgatggg aaaaagagaa aagaaacaag gggaatttgg 9060
aaaggccaag ggcagccgcg ccatctggta tatgtggcta ggggctagat ttctagagtt 9120
cgaagccctt ggattcttga acgaggatca ctggatgggg agagagaact caggaggtgg 9180
tgttgaaggg ctgggattac aaagactcgg atatgtccta gaagagatga gtcgcatacc 9240
aggaggaagg atgtatgcag atgacactgc tggctgggac acccgcatca gcaggtttga 9300
tctggagaat gaagctctaa tcaccaacca aatggagaaa gggcacaggg ccttggcatt 9360
ggccataatc aagtacacat accaaaacaa agtggtaaag gtccttagac cagctgaaaa 9420
agggaaaaca gttatggaca ttatttcgag acaagaccaa agggggagcg gacaagttgt 9480
cacttacgct cttaacacat ttaccaacct agtggtgcaa ctcattcgga atatggaggc 9540
tgaggaagtt ctagagatgc aagacttgtg gctgctgcgg aggtcagaga aagtgaccaa 9600
ctggttgcag agcaacggat gggataggct caaacgaatg gcagtcagtg gagatgattg 9660
cgttgtgaag ccaattgatg ataggtttgc acatgccctc aggttcttga atgatatggg 9720
aaaagttagg aaggacacac aagagtggaa accctcaact ggatgggaca actgggaaga 9780
agttccgttt tgctcccacc acttcaacaa gctccatctc aaggacggga ggtccattgt 9840
ggttccctgc cgccaccaag atgaactgat tggccgggcc cgcgtctctc caggggcggg 9900
atggagcatc cgggagactg cttgcctagc aaaatcatat gcgcaaatgt ggcagctcct 9960
ttatttccac agaagggacc tccgactgat ggccaatgcc atttgttcat ctgtgccagt 10020
tgactgggtt ccaactggga gaactacctg gtcaatccat ggaaagggag aatggatgac 10080
cactgaagac atgcttgtgg tgtggaacag agtgtggatt gaggagaacg accacatgga 10140
agacaagacc ccagttacga aatggacaga cattccctat ttgggaaaaa gggaagactt 10200
gtggtgtgga tctctcatag ggcacagacc gcgcaccacc tgggctgaga acattaaaaa 10260
tacagtcaac atggtgcgca ggatcatagg tgatgaagaa aagtacatgg actacctatc 10320
cacccaagtt cgctacttgg gtgaagaagg gtctacacct ggagtgctgt gagcaccaat 10380
cttaatgttg tcaggcctgc tagtcagcca cagcttgggg aaagctgtgc agcctgtgac 10440
ccctccagga gaagctgggt aaccaagcct atagtcaggc cgagaacgcc atggcacgga 10500
agaagccatg ctgcctgtga gcccctcaga ggacactgag tcaaaaaacc ccacgcgctt 10560
ggaggcgcag gatgggaaaa gaaggtggcg accttcccca cccttcaatc tggggcctga 10620
actggagatc agctgtggat ctccagaaga gggactagtg gttagaggag accccccgga 10680
aaacgcaaaa cagcatattg acgctgggaa agaccagaga ctccatgagt ttccaccacg 10740
ctggccgcca ggcacagatc gccgaatagc ggcggccggt gtggggaaat cca 10793
<210> 4
<211> 10675
<212> DNA
<213> Zika病毒
<400> 4
gttgttgatc tgtgtgaatc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60
gtatcaacag gttttatttt ggatttggaa acgagagttt ctggtcatga aaaacccaaa 120
aaagaaatcc ggaggattcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtgag 180
cccctttggg ggcttgaaga ggctgccagc cggacttctg ctgggtcatg ggcccatcag 240
gatggtcttg gcgattctag cctttttgag attcacggca atcaagccat cactgggtct 300
catcaataga tggggttcag tggggaaaaa agaggctatg gaaacaataa agaagttcaa 360
gaaagatctg gctgccatgc tgagaataat caatgctagg aaggagaaga agagacgagg 420
cgcagatact agtgtcggaa ttgttggcct cctgctgacc acagctatgg cagcggaggt 480
cactagacgt gggagtgcat actatatgta cttggacaga aacgatgctg gggaggccat 540
atcttttcca accacattgg ggatgaataa gtgttatata cagatcatgg atcttggaca 600
catgtgtgat gccaccatga gctatgaatg ccctatgctg gatgaggggg tggaaccaga 660
tgacgtcgat tgttggtgca acacgacgtc aacttgggtt gtgtacggaa cctgccatca 720
caaaaaaggt gaagcacgga gatctagaag agctgtgacg ctcccctccc attccaccag 780
gaagctgcaa acgcggtcgc aaacctggtt ggaatcaaga gaatacacaa agcacttgat 840
tagagtcgaa aattggatat tcaggaaccc tggcttcgcg ttagcagcag ctgccatcgc 900
ttggcttttg ggaagctcaa cgagccaaaa agtcatatac ttggtcatga tactgctgat 960
tgccccggca tacagcatca ggtgcatagg agtcagcaat agggactttg tggaaggtat 1020
gtcaggtggg acttgggttg atgttgtctt ggaacatgga ggttgtgtca ccgtaatggc 1080
acaggacaaa ccgactgtcg acatagagct ggttacaaca acagtcagca acatggcgga 1140
ggtaagatcc tactgctatg aggcatcaat atcagacatg gcttctgaca gccgctgccc 1200
aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac 1260
gttagtggac agaggctggg gaaatggatg tggacttttt ggcaaaggga gcctggtgac 1320
atgcgctaag tttgcatgct ccaagaaaat gaccgggaag agcatccagc cagagaatct 1380
ggagtaccgg ataatgctgt cagttcatgg ctcccagcac agtgggatga tcgttaatga 1440
cacaggacat gaaactgatg agaatagagc gaaagttgag ataacgccca attcaccgag 1500
agccgaagcc accctggggg gttttggaag cctaggactt gattgtgaac cgaggacagg 1560
ccttgacttt tcagatttgt attacttgac tatgaataac aagcactggt tggttcacaa 1620
ggagtggttc cacgacattc cattaccttg gcacgctggg gcagacaccg gaactccaca 1680
ctggaacaac aaagaagcac tggtagagtt caaggacgca catgccaaaa ggcaaactgt 1740
cgtggttcta gggagtcaag aaggagcagt tcacacggcc cttgctggag ctctggaggc 1800
tgagatggat ggtgcaaagg gaaggctgtc ctctggccac ttgaaatgtc gcctgaaaat 1860
ggataaactt agattgaagg gcgtgtcata ctccttgtgt actgcagcgt tcacattcac 1920
caagatcccg gctgaaacac tgcacgggac agtcacagtg gaggtacagt acgcagggac 1980
agatggacct tgcaaggttc cagctcagat ggcggtggac atgcaaactc tgaccccagt 2040
tgggaggttg ataaccgcta accccgtaat cactgaaagc actgagaact ctaagatgat 2100
gctggaactt gatccaccat ttggggactc ttacattgtc ataggagtcg gggagaagaa 2160
gatcacccac cactggcaca ggagtggcag caccattgga aaagcatttg aagccactgt 2220
gagaggtgcc aagagaatgg cagtcttggg agacacagcc tgggactttg gatcagttgg 2280
aggcgctctc aactcattgg gcaagggcat ccatcaaatt tttggagcag ctttcaaatc 2340
attgtttgga ggaatgtcct ggttctcaca aattctcatt ggaacgttgc tgatgtggtt 2400
gggtctgaac acaaagaatg gatctatttc ccttatgtgc ttggccttag ggggagtgtt 2460
gatcttctta tccacagccg tctctgctga tgtggggtgc tcggtggact tctcaaagaa 2520
ggagacgaga tgcggtacag gggtgttcgt ctataacgac gttgaagcct ggagggacag 2580
gtacaagtac catcctgact ccccccgtag attggcagca gcagtcaagc aagcctggga 2640
agatggtatc tgcgggatct cctctgtttc aagaatggaa aacatcatgt ggagatcagt 2700
agaaggggag ctcaacgcaa tcctggaaga gaatggagtt caactgacgg tcgttgtggg 2760
atctgtaaaa aaccccatgt ggagaggtcc acagagattg cccgtgcctg tgaacgagct 2820
gccccacggc tggaaggctt gggggaaatc gtatttcgtc agagcagcaa agacaaataa 2880
cagctttgtc gtggatggtg acacactgaa ggaatgccca ctcaaacata gagcatggaa 2940
cagctttctt gtggaggatc atgggttcgg ggtatttcac actagtgtct ggctcaaggt 3000
tagagaagat tattcattag agtgtgatcc agccgttatt ggaacagctg ttaagggaaa 3060
ggaggctgta cacagtgatc taggctactg gattgagagt gagaagaatg acacatggag 3120
gctgaagagg gcccatctga tcgagatgaa aacatgtgaa tggccaaagt cccacacatt 3180
gtggacagat ggaatagaag agagtgatct gatcataccc aagtctttag ctgggccact 3240
cagccatcac aataccagag agggctacag gacccaaatg aaagggccat ggcacagtga 3300
agagcttgaa attcggtttg aggaatgccc aggcactaag gtccacgtgg aggaaacatg 3360
tggaacaaga ggaccatctc tgagatcaac cactgcaagc ggaagggtga tcgaggaatg 3420
gtgctgcagg gagtgcacaa tgcccccact gtcgttccgg gctaaagatg gctgttggta 3480
tggaatggag ataaggccca ggaaagaacc agaaagcaac ttagtaaggt caatggtgac 3540
tgcaggatca actgatcaca tggaccactt ctcccttgga gtgcttgtga tcctgctcat 3600
ggtgcaggaa gggctgaaga agagaatgac cacaaagatc atcataagca catcaatggc 3660
agtgctggta gctatgatcc tgggaggatt ttcaatgagt gacctggcta agcttgcaat 3720
tttgatgggt gccaccttcg cggaaatgaa cactggagga gatgtagctc atctggcgct 3780
gatagcggca ttcaaagtca gaccagcgtt gctggtatct ttcatcttca gagctaattg 3840
gacaccccgt gaaagcatgc tgctggcctt ggcctcgtgt cttttgcaaa ctgcgatctc 3900
cgccttggaa ggcgacctga tggttctcat caatggtttt gctttggcct ggttggcaat 3960
acgagcgatg gttgttccac gcactgataa catcaccttg gcaatcctgg ctgctctgac 4020
accactggcc cggggcacac tgcttgtggc gtggagagca ggccttgcta cttgcggggg 4080
gtttatgctc ctctctctga agggaaaagg cagtgtgaag aagaacttac catttgtcat 4140
ggccctggga ctaaccgctg tgaggctggt cgaccccatc aacgtggtgg gactgctgtt 4200
gctcacaagg agtgggaagc ggagctggcc ccctagcgaa gtactcacag ctgttggcct 4260
gatatgcgca ttggctggag ggttcgccaa ggcagatata gagatggctg ggcccatggc 4320
cgcggtcggt ctgctaattg tcagttacgt ggtctcagga aagagtgtgg acatgtacat 4380
tgaaagagca ggtgacatca catgggaaaa agatgcggaa gtcactggaa acagtccccg 4440
gctcgatgtg gcgctagatg agagtggtga tttctccctg gtggaggatg acggtccccc 4500
catgagagag atcatactca aggtggtcct gatgaccatc tgtggcatga acccaatagc 4560
catacccttt gcagctggag cgtggtacgt atacgtgaag actggaaaaa ggagtggtgc 4620
tctatgggat gtgcctgctc ccaaggaagt aaaaaagggg gagaccacag atggagtgta 4680
cagagtaatg actcgtagac tgctaggttc aacacaagtt ggagtgggag ttatgcaaga 4740
gggggtcttt cacactatgt ggcacgtcac aaaaggatcc gcgctgagaa gcggtgaagg 4800
gagacttgat ccatactggg gagatgtcaa gcaggatctg gtgtcatact gtggtccatg 4860
gaagctagat gccgcctggg atgggcacag cgaggtgcag ctcttggccg tgccccccgg 4920
agagagagcg aggaacatcc agactctgcc cggaatattt aagacaaagg atggggacat 4980
tggagcggtt gcgctggatt acccagcagg aacttcagga tctccaatcc tagacaagtg 5040
tgggagagtg ataggacttt atggcaatgg ggtcgtgatc aaaaacggga gttatgttag 5100
tgccatcacc caagggagga gggaggaaga gactcctgtt gagtgcttcg agccctcgat 5160
gctgaagaag aagcagctaa ctgtcttaga cttgcatcct ggagctggga aaaccaggag 5220
agttcttcct gaaatagtcc gtgaagccat aaaaacaaga ctccgtactg tgatcttagc 5280
tccaaccagg gttgtcgctg ctgaaatgga ggaggccctt agagggcttc cagtgcgtta 5340
tatgacaaca gcagtcaatg tcacccactc tggaacagaa atcgtcgact taatgtgcca 5400
tgccaccttc acttcacgtc tactacagcc aatcagagtc cccaactata atctgtatat 5460
tatggatgag gcccacttca cagatccctc aagtatagca gcaagaggat acatttcaac 5520
aagggttgag atgggcgagg cggctgccat cttcatgacc gccacgccac caggaacccg 5580
tgacgcattt ccggactcca actcaccaat tatggacacc gaagtggaag tcccagagag 5640
agcctggagc tcaggctttg attgggtgac ggatcattct ggaaaaacag tttggtttgt 5700
tccaagcgtg aggaacggca atgagatcgc agcttgtctg acaaaggctg gaaaacgggt 5760
catacagctc agcagaaaga cttttgagac agagttccag aaaacaaaac atcaagagtg 5820
ggactttgtc gtgacaactg acatttcaga gatgggcgcc aactttaaag ctgaccgtgt 5880
catagattcc aggagatgcc taaagccggt catacttgat ggcgagagag tcattctggc 5940
tggacccatg cctgtcacac atgccagcgc tgcccagagg agggggcgca taggcaggaa 6000
tcccaacaaa cctggagatg agtatctgta tggaggtggg tgcgcagaga ctgacgaaga 6060
ccatgcacac tggcttgaag caagaatgct ccttgacaat atttacctcc aagatggcct 6120
catagcctcg ctctatcgac ctgaggccga caaagtagca gccattgagg gagagttcaa 6180
gcttaggacg gagcaaagga agacctttgt ggaactcatg aaaagaggag atcttcctgt 6240
ttggctggcc tatcaggttg catctgccgg aataacctac acagatagaa gatggtgctt 6300
tgatggcacg accaacaaca ccataatgga agacagtgtg ccggcagagg tgtggaccag 6360
acacggagag aaaagagtgc tcaaaccgag gtggatggac gccagagttt gttcagatca 6420
tgcggccctg aagtcattca aggagtttgc cgctgggaaa agaggagcgg cttttggagt 6480
gatggaagcc ctgggaacac tgccaggaca catgacagag agattccagg aagccattga 6540
caacctcgct gtgctcatgc gggcagagac tggaagcagg ccttacaaag ccgcggcggc 6600
ccaattgccg gagaccctag agaccataat gcttttgggg ttgctgggaa cagtctcgct 6660
gggaatcttc ttcgtcttga tgaggaacaa gggcataggg aagatgggct ttggaatggt 6720
gactcttggg gccagcgcat ggctcatgtg gctctcggaa attgagccag ccagaattgc 6780
atgtgtcctc attgttgtgt tcctattgct ggtggtgctc atacctgagc cagaaaagca 6840
aagatctccc caggacaacc aaatggcaat catcatcatg gtagcagtag gtcttctggg 6900
cttgattacc gccaatgaac tcggatggtt ggagagaaca aagagtgacc taagccatct 6960
aatgggaagg agagaggagg gggcaaccat aggattctca atggacattg acctgcggcc 7020
agcctcagct tgggccatct atgctgcctt gacaactttc attaccccag ccgtccaaca 7080
tgcagtgacc acctcataca acaactactc cttaatggcg atggccacgc aagctggagt 7140
gttgtttggc atgggcaaag ggatgccatt ctacgcatgg gactttggag tcccgctgct 7200
aatgataggt tgctactcac aattaacacc cctgacccta atagtggcca tcattttgct 7260
cgtggcgcac tacatgtact tgatcccagg gctgcaggca gcagctgcgc gtgctgccca 7320
gaagagaacg gcagctggca tcatgaagaa ccctgttgtg gatggaatag tggtgactga 7380
cattgacaca atgacaattg acccccaagt ggagaaaaag atgggacagg tgctactcat 7440
agcagtagcc gtctccagcg ccatactgtc gcggaccgcc tgggggtggg gggaggctgg 7500
ggctctgatc acagccgcaa cttccacttt gtgggaaggc tctccgaaca agtactggaa 7560
ctcctctaca gccacttcac tgtgtaacat ttttagggga agttacttgg ctggagcttc 7620
tctaatctac acagtaacaa gaaacgctgg cttggtcaag agacgtgggg gtggaacagg 7680
agagaccctg ggagagaaat ggaaggcccg cttgaaccag atgtcggccc tggagttcta 7740
ctcctacaaa aagtcaggca tcaccgaggt gtgcagagaa gaggcccgcc gcgccctcaa 7800
ggacggtgtg gcaacgggag gccatgctgt gtcccgagga agtgcaaagc tgagatggtt 7860
ggtggagcgg ggatacctgc agccctatgg aaaggtcatt gatcttggat gtggcagagg 7920
gggctggagt tactacgtcg ccaccatccg caaagttcaa gaagtgaaag gatacacaaa 7980
aggaggccct ggtcatgaag aacccgtgtt ggtgcaaagc tatgggtgga acatagtccg 8040
tcttaagagt ggggtggacg tctttcatat ggcggctgag ccgtgtgaca cgttgctgtg 8100
tgacataggt gagtcatcat ctagtcctga agtggaagaa gcacggacgc tcagagtcct 8160
ctccatggtg ggggattggc ttgaaaaaag accaggagcc ttttgtataa aagtgttgtg 8220
cccatacacc agcactatga tggaaaccct ggagcgactg cagcgtaggt atgggggagg 8280
actggtcaga gtgccactct cccgcaactc tacacatgag atgtactggg tctctggagc 8340
gaaaagcaac accataaaaa gtgtgtccac cacgagccag ctcctcttgg ggcgcatgga 8400
cgggcctagg aggccagtga aatatgagga ggatgtgaat ctcggctctg gcacgcgggc 8460
tgtggtaagc tgcgctgaag ctcccaacat gaagatcatt ggtaaccgca ttgaaaggat 8520
ccgcagtgag cacgcggaaa cgtggttctt tgacgagaac cacccatata ggacatgggc 8580
ttaccatgga agctatgagg cccccacaca agggtcagcg tcctctctaa taaacggggt 8640
tgtcaggctc ctgtcaaaac cctgggatgt ggtgactgga gtcacaggaa tagccatgac 8700
cgacaccaca ccgtatggtc agcaaagagt tttcaaggaa aaagtggaca ctagggtgcc 8760
agacccccaa gaaggcactc gtcaggttat gagcatggtc tcttcctggt tgtggaaaga 8820
gctaggcaaa cacaaacggc cacgagtctg caccaaagaa gagttcatca acaaggttcg 8880
tagcaatgca gcattagggg caatatttga agaggaaaaa gagtggaaga ctgcagtgga 8940
agctgtgaac gatccaaggt tctgggctct agtggacaag gaaagagagc accacctgag 9000
aggagagtgc cagagctgtg tgtacaacat gatgggaaaa agagaaaaga aacaagggga 9060
atttggaaag gccaagggca gccgcgccat ctggtatatg tggctagggg ctagatttct 9120
agagttcgaa gcccttggat tcttgaacga ggatcactgg atggggagag agaactcagg 9180
aggtggtgtt gaagggctgg gattacaaag actcggatat gtcctagaag agatgagtcg 9240
tataccagga ggaaggatgt atgcagatga cactgctggc tgggacaccc gcattagcag 9300
gtttgatctg gagaatgaag ctctaatcac caaccaaatg gagaaagggc acagggcctt 9360
ggcattggcc ataatcaagt acacatacca aaacaaagtg gtaaaggtcc ttagaccagc 9420
tgaaaaaggg aaaacagtta tggacattat ttcgagacaa gaccaaaggg ggagcggaca 9480
agttgtcact tacgctctta acacatttac caacctagtg gtgcaactca ttcggaatat 9540
ggaggctgag gaagttctag agatgcaaga cttgtggctg ctgcggaggt cagagaaagt 9600
gaccaactgg ttgcagagca acggatggga taggctcaaa cgaatggcag tcagtggaga 9660
tgattgcgtt gtgaagccaa ttgatgatag gtttgcacat gccctcaggt tcttgaatga 9720
tatgggaaaa gttaggaagg acacacaaga gtggaaaccc tcaactggat gggacaactg 9780
ggaagaagtt ccgttttgct cccaccactt caacaagctc catctcaagg acgggaggtc 9840
cattgtggtt ccctgccgcc accaagatga actgattggc cgggcccgcg tctctccagg 9900
ggcgggatgg agcatccggg agactgcttg cctagcaaaa tcatatgcgc aaatgtggca 9960
gctcctttat ttccacagaa gggacctccg actgatggcc aatgccattt gttcatctgt 10020
gccagttgac tgggttccaa ctgggagaac tacctggtca atccatggaa agggagaatg 10080
gatgaccact gaagacatgc ttgtggtgtg gaacagagtg tggattgagg agaacgacca 10140
catggaagac aagaccccag ttacgaaatg gacagacatt ccctatttgg gaaaaaggga 10200
agacttgtgg tgtggatctc tcatagggca cagaccgcgc accacctggg ctgagaacat 10260
taaaaacaca gtcaacatgg tgcgcaggat cataggtgat gaagaaaagt acatggacta 10320
cctatccacc caagttcgct acttgggtga agaagggtct acacctggag tgctgtaagc 10380
accaatctta atgttgtcag gcctgctagt cagccacagc ttggggaaag ctgtgcagcc 10440
tgtgaccccc ccaggagaag ctgggaaacc aagcctatag tcaggccgag aacgccatgg 10500
cacggaagaa gccatgctgc ctgtgagccc ctcagaggac actgagtcaa aaaaccccac 10560
gcgcttggag gcgcaggatg ggaaaagaag gtggcgacct tccccaccct tcaatctggg 10620
gcctgaactg gagatcagct gtggatctcc agaagaggga ctagtggtta gagga 10675
<210> 5
<211> 10676
<212> DNA
<213> Zika病毒
<400> 5
gttgttactg ttgctgactc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60
gtatcaacag gttttatttg gatttggaaa cgagagtttc tggtcatgaa aaacccaaaa 120
aagaaatccg gaggattccg gattgtcaat atgctaaaac gcggagtagc ccgtgtgagc 180
ccctttgggg gcttgaagag gctgccagcc ggacttctgc tgggtcatgg gcccatcagg 240
atggtcttgg caattctagc ctttttgaga ttcacggcaa tcaagccatc actgggtctc 300
atcaatagat ggggttcagt ggggaaaaaa gaggctatgg aaataataaa gaagttcaag 360
aaagatctgg ctgccatgct gagaataatc aatgctagga aggagaagaa gagacgaggc 420
gcagatacta gtgtcggaat tgttggcctc ctgctgacca cagctatggc agcggaggtc 480
actagacgtg ggagtgcata ctatatgtac ttggacagaa acgatgctgg ggaggccata 540
tcttttccaa ccacattggg gatgaataag tgttatatac agatcatgga tcttggacac 600
atgtgtgatg ccaccatgag ctatgaatgc cctatgctgg atgagggggt ggaaccagat 660
gacgtcgatt gttggtgcaa cacgacgtca acttgggttg tgtacggaac ctgccatcac 720
aaaaaaggtg aagcacggag atctagaaga gctgtgacgc tcccctccca ttccactagg 780
aagctgcaaa cgcggtcgca aacctggttg gaatcaagag aatacacaaa gcacttgatt 840
agagtcgaaa attggatatt caggaaccct ggcttcgcgt tagcagcagc tgccatcgct 900
tggcttttgg gaagctcaac gagccaaaaa gtcatatact tggtcatgat actgctgatt 960
gccccggcat acagcatcag gtgcatagga gtcagcaata gggactttgt ggaaggtatg 1020
tcaggtggga cttgggttga tgttgtcttg gaacatggag gttgtgtcac cgtaatggca 1080
caggacaaac cgactgtcga catagagctg gttacaacaa cagtcagcaa catggcggag 1140
gtaagatcct actgctatga ggcatcaata tcagacatgg cttcggacag ccgctgccca 1200
acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtctg caaaagaacg 1260
ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag tctggtgaca 1320
tgcgctaagt ttgcatgctc caagaaaatg accgggaaga gcatccagcc agagaatctg 1380
gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgat cgttaatgac 1440
acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa ttcaccaaga 1500
gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc gaggacaggc 1560
cttgactttt cagatttgta ttacttgact atgaataaca agcactggtt ggttcacaag 1620
gagtggttcc acgacattcc attaccttgg cacgctgggg cagacaccgg aactccacac 1680
tggaacaaca aagaagcact ggtagagttc aaggacgcac atgccaaaag gcaaactgtc 1740
gtggttctag ggagtcaaga aggagcagtt cacacggccc ttgctggagc tctggaggct 1800
gagatggatg gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg cctgaaaatg 1860
gataaactta gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt cacattcacc 1920
aagatcccgg ctgaaacact gcacgggaca gtcacagtgg aggtacagta cgcagggaca 1980
gatggacctt gcaaggttcc agctcagatg gcggtggaca tgcaaactct gaccccagtt 2040
gggaggttga taaccgctaa ccccgtaatc actgaaagca ctgagaactc taagatgatg 2100
ctggaacttg atccaccatt tggggactct tacattgtca taggagtcgg ggagaagaag 2160
atcacccacc actggcacag gagtggcagc accattggaa aagcatttga agccactgtg 2220
agaggtgcca agagaatggc agtcttggga gacacagcct gggactttgg atcagttgga 2280
ggcgctctca actcattggg caagggcatc catcaaattt ttggagcagc tttcaaatca 2340
ttgtttggag gaatgtcctg gttctcacaa attctcattg gaacgttgct gatgtggttg 2400
ggtctgaaca caaagaatgg atctatttcc cttatgtgct tggccttagg gggagtgttg 2460
atcttcttat ccacagccgt ctctgctgat gtggggtgct cggtggactt ctcaaagaag 2520
gagacgagat gcggtacagg ggtgttcgtc tataacgacg ttgaagcctg gagggacagg 2580
tacaagtacc atcctgactc cccccgtaga ttggcagcag cagtcaagca agcctgggaa 2640
gatggtatct gcgggatctc ctctgtttca agaatggaaa acatcatgtg gagatcagta 2700
gaaggggagc tcaacgcaat cctggaagag aatggagttc aactgacggt cgttgtggga 2760
tctgtaaaaa accccatgtg gagaggtcca cagagattgc ccgtgcctgt gaacgagctg 2820
ccccacggct ggaaggcttg ggggaaatcg cacttcgtca gagcagcaaa gacaaataac 2880
agctttgtcg tggatggtga cacactgaag gaatgcccac tcaaacatag agcatggaac 2940
agctttcttg tggaggatca tgggttcggg gtatttcaca ctagtgtctg gctcaaggtt 3000
agagaagatt attcattaga gtgtgatcca gccgttattg gaacagctgt taagggaaag 3060
gaggctgtac acagtgatct aggctactgg attgagagtg agaagaatga cacatggagg 3120
ctgaagaggg cccatctgat cgagatgaaa acatgtgaat ggccaaagtc ccacacattg 3180
tggacagatg gaatagaaga gagtgatctg atcataccca agtctttagc tgggccactc 3240
agccatcaca ataccagaga gggctacagg acccaaatga aagggccatg gcacagtgaa 3300
gagcttgaaa ttcggtttga ggaatgccca ggcactaagg tccacgtgga ggaaacatgt 3360
ggaacaagag gaccatctct gagatcaacc actgcaagcg gaagggtgat cgaggaatgg 3420
tgctgcaggg agtgcacaat gcccccactg tcgttccggg ctaaagatgg ctgttggtat 3480
ggaatggaga taaggcccag gaaagaacca gaaagcaact tagtaaggtc aatggtgact 3540
gcaggatcaa ctgatcacat ggatcacttc tcccttggag tgcttgtgat tctgctcatg 3600
gtgcaggaag ggctgaagaa gagaatgacc acaaagatca tcataagcac atcaatggca 3660
gtgctggtag ctatgatcct gggaggattt tcaatgagtg acctggctaa gcttgcaatt 3720
ttgatgggtg ccaccttcgc ggaaatgaac actggaggag atgtagctca tctggcgctg 3780
atagcggcat tcaaagtcag accagcgttg ctggtatctt tcatcttcag agctaattgg 3840
acaccccgtg aaagcatgct gctggccttg gcctcgtgtc ttttgcaaac tgcgatctcc 3900
gccttggaag gcgacctgat ggttctcatc aatggttttg ctttggcctg gttggcaata 3960
cgagcgatgg ttgttccacg cactgataac atcaccttgg caatcctggc tgctctgaca 4020
ccactggccc ggggcacact gcttgtggcg tggagagcag gccttgctac ttgcgggggg 4080
tttatgctcc tctctctgaa gggaaaaggc agtgtgaaga agaacttacc atttgtcatg 4140
gccctgggac taaccgctgt gaggctggtc gaccccatca acgtggtggg gctgctgttg 4200
ctcacaagga gtgggaagcg gagctggccc cctagcgaag tactcacagc tgttggcctg 4260
atatgcgcat tggctggagg gttcgccaag gcagatatag agatggctgg gcccatggcc 4320
gcggtcggtc tgctaattgt cagttacgtg gtctcaggaa agagtgtgga catgtacatt 4380
gaaagagcag gtgacatcac atgggaaaaa gatgcggaag tcactggaaa cagtccccgg 4440
ctcgatgtgg cgctagatga gagtggtgat ttctccctgg tggaggatga cggtcccccc 4500
atgagagaga tcatactcaa ggtggtcctg atgaccatct gtggcatgaa cccaatagcc 4560
ataccctttg cagctggagc gtggtacgta tacgtgaaga ctggaaaaag gagtggtgct 4620
ctatgggatg tgcctgctcc caaggaagta aaaaaggggg agaccacaga tggagtgtac 4680
agagtaatga ctcgtagact gctaggttca acacaagttg gagtgggagt tatgcaagag 4740
ggggtctttc acactatgtg gcacgtcaca aaaggatccg cgctgagaag cggtgaaggg 4800
agacttgatc catactgggg agatgtcaag caggatctgg tgtcatactg tggtccatgg 4860
aagctagatg ccgcctggga cgggcacagc gaggtgcagc tcttggccgt gccccccgga 4920
gagagagcga ggaacatcca gactctgccc ggaatattta agacaaagga tggggacatt 4980
ggagcggttg cgctggatta cccagcagga acttcaggat ctccaatcct agacaagtgt 5040
gggagagtga taggacttta tggcaatggg gtcgtgatca aaaatgggag ttatgttagt 5100
gccatcaccc aagggaggag ggaggaagag actcctgttg agtgcttcga gccttcgatg 5160
ctgaagaaga agcagctaac tgtcttagac ttgcatcctg gagctgggaa aaccaggaga 5220
gttcttcctg aaatagtccg tgaagccata aaaacaagac tccgtactgt gatcttagct 5280
ccaaccaggg ttgtcgctgc tgaaatggag gaagccctta gagggcttcc agtgcgttat 5340
atgacaacag cagtcaatgt cacccactct ggaacagaaa tcgtcgactt aatgtgccat 5400
gccaccttca cttcacgtct actacagcca atcagagtcc ccaactataa tctgtatatt 5460
atggatgagg cccacttcac agatccctca agtatagcag caagaggata catttcaaca 5520
agggttgaga tgggcgaggc ggctgccatc ttcatgaccg ccacgccacc aggaacccgt 5580
gacgcatttc cggactccaa ctcaccaatt atggacaccg aagtggaagt cccagagaga 5640
gcctggagct caggctttga ttgggtgacg gattattctg gaaaaacagt ttggtttgtt 5700
ccaagcgtga ggaacggcaa tgagatcgca gcttgtctga caaaggctgg aaaacgggtc 5760
atacagctca gcagaaagac ttttgagaca gagttccaga aaacaaaaca tcaagagtgg 5820
gactttgtcg tgacaactga catttcagag atgggcgcca actttaaagc tgaccgtgtc 5880
atagattcca ggagatgcct aaagccggtc atacttgatg gcgagagagt cattctggct 5940
ggacccatgc ctgtcacaca tgccagcgct gcccagagga gggggcgcat aggcaggaat 6000
cccaacaaac ctggagatga gtatctgtat ggaggtgggt gcgcagagac tgacgaagac 6060
catgcacact ggcttgaagc aagaatgctc cttgacaata tttacctcca agatggcctc 6120
atagcctcgc tctatcgacc tgaggccgac aaagtagcag ccattgaggg agagttcaag 6180
cttaggacgg agcaaaggaa gacctttgtg gaactcatga aaagaggaga tcttcctgtt 6240
tggctggcct atcaggttgc atctgccgga ataacctaca cagatagaag atggtgcttt 6300
gatggcacga ccaacaacac cataatggaa gacagtgtgc cggcagaggt gtggaccaga 6360
cacggagaga aaagagtgct caaaccgagg tggatggacg ccagagtttg ttcagatcat 6420
gcggccctga agtcattcaa ggagtttgcc gctgggaaaa gaggagcggc ttttggagtg 6480
atggaagccc tgggaacact gccaggacac atgacagaga gattccagga agccattgac 6540
aacctcgctg tgctcatgcg ggcagagact ggaagcaggc cttacaaagc cgcggcggcc 6600
caattgccgg agaccctaga gaccattatg cttttggggt tgctgggaac agtctcgctg 6660
ggaatctttt tcgtcttgat gaggaacaag ggcataggga agatgggctt tggaatggtg 6720
actcttgggg ccagcgcatg gctcatgtgg ctctcggaaa ttgagccagc cagaattgca 6780
tgtgtcctca ttgttgtgtt cctattgctg gtggtgctca tacctgagcc agaaaagcaa 6840
agatctcccc aggacaacca aatggcaatc atcatcatgg tagcagtagg tcttctgggc 6900
ttgattaccg ccaatgaact cggatggttg gagagaacaa agagtgacct aagccatcta 6960
atgggaagga gagaggaggg ggcaaccatg ggattctcaa tggacattga cctgcggcca 7020
gcctcagctt gggccatcta tgctgccttg acaactttca ttaccccagc cgtccaacat 7080
gcagtgacca cttcatacaa caactactcc ttaatggcga tggccacgca agctggagtg 7140
ttgtttggta tgggcaaagg gatgccattc tacgcatggg actttggagt cccgctgcta 7200
atgataggtt gctactcaca attaacgccc ctgaccctaa tagtggccat cattttgctc 7260
gtggcgcact acatgtactt gatcccaggg ctgcaggcag cagctgcgcg tgctgcccag 7320
aagagaacgg cagctggcat catgaagaac cctgttgtgg atggaatagt ggtgactgac 7380
attgacacaa tgacaattga cccccaagtg gagaaaaaga tgggacaggt gctactcatg 7440
gcagtagccg tctccagcgc catactgtcg cggaccgcct gggggtgggg ggaggctggg 7500
gccctgatca cagccgcaac ttccactttg tgggaaggct ctccgaacaa gtactggaac 7560
tcctctacag ccacttcact gtgtaacatt tttaggggaa gttacttggc tggagcttct 7620
ctaatctaca cagtaacaag aaacgctggc ttggtcaaga gacgtggggg tggaacagga 7680
gagaccctgg gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac 7740
tcctacaaaa agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag 7800
gacggtgtgg caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg 7860
gtggagcggg gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg 7920
ggctggagtt actacgccgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa 7980
ggaggccctg gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt 8040
cttaagagtg gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt 8100
gacataggtg agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc 8160
tccatggtgg gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc 8220
ccatacacca gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga 8280
ctggtcagag tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg 8340
aaaagcaaca ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac 8400
gggcctagga ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct 8460
gtggtaagct gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc 8520
cgcagtgagc acgcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct 8580
taccatggaa gctatgaggc ccccacacaa gggtcagcgt cctctctaat aaacggggtt 8640
gtcaggctcc tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc 8700
gacaccacac cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca 8760
gacccccaag aaggcactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag 8820
ctaggcaaac acaaacggcc acgagtctgt accaaagaag agttcatcaa caaggttcgt 8880
agcaatgcag cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa 8940
gctgtgaacg atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga 9000
ggagagtgcc agagttgtgt gtacaacatg atgggaaaaa gagaaaagaa acaaggggaa 9060
tttggaaagg ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta 9120
gagttcgaag cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga 9180
ggtggtgttg aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgc 9240
ataccaggag gaaggatgta tgcagatgac actgctggct gggacacccg catcagcagg 9300
tttgatctgg agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg 9360
gcattggcca taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct 9420
gaaaaaggga agacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa 9480
gttgtcactt acgctcttaa cacatttacc aacctagtgg tgcaactcat tcggaatatg 9540
gaggctgagg aagttctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg 9600
accaactggt tgcagagcaa cggatgggat aggctcaaac gaatggcagt cagtggagat 9660
gattgcgttg tgaagccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat 9720
atgggaaaag ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg 9780
gaagaagttc cgttttgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc 9840
attgtggttc cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg 9900
gcgggatgga gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag 9960
ctcctttatt tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg 10020
ccagttgact gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg 10080
atgaccactg aagacatgct tgtggtgtgg aacagagtgt ggattgagga gaacgaccac 10140
atggaagaca agaccccagt tacgaaatgg acagacattc cctatttggg aaaaagggaa 10200
gacttgtggt gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt 10260
aaaaacacag tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac 10320
ctatccaccc aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtaagca 10380
ccaatcttaa tgttgtcagg cctgctagtc agccacagct tggggaaagc tgtgcagcct 10440
gtgacccccc caggagaagc tgggaaacca agcctatagt caggccgaga acgccatggc 10500
acggaagaag ccatgctgcc tgtgagcccc tcagaggaca ctgagtcaaa aaaccccacg 10560
cgcttggagg cgcaggatgg gaaaagaagg tggcgacctt ccccaccctt caatctgggg 10620
cctgaactgg agatcagctg tggatctcca gaagagggac tagtggttag aggaga 10676
<210> 6
<211> 10808
<212> DNA
<213> Zika病毒
<400> 6
agttgttgat ctgtgtgaat cagactgcga cagttcgagt ttgaagcgaa agctagcaac 60
agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 120
aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 180
gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca 240
ggatggtctt ggcaattcta gcctttttga gattcacggc aatcaagcca tcactgggtc 300
tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca 360
agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 420
gcgcagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg 480
tcactagacg tgggagtgca tactatatgt acttggacag aaacgatgct ggggaggcca 540
tatcttttcc aaccacattg gggatgaata agtgttatat acagatcatg gatcttggac 600
acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtggaaccag 660
atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc 720
acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta 780
ggaagctgca aacgcggtcg caaacctggt tggaatcaag agaatacaca aagcacttga 840
ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg 900
cttggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga 960
ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta 1020
tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg 1080
cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1140
aggtaagatc ctactgctat gaggcatcaa tatcagacat ggcttcggac agccgctgcc 1200
caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa 1260
cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1320
catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1380
tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1440
acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1500
gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag 1560
gccttgactt ttcagatttg tattacttga ctatgaataa caagcactgg ttggtccaca 1620
aggagtggtt ccacgacatt ccattacctt ggcacgctgg ggcagacacc ggaactccac 1680
actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg 1740
tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1800
ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1860
tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca 1920
ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1980
cagatggacc ttgcaaggtt ccagctcaga tggcggtgga catgcaaact ctgaccccag 2040
ttgggaggtt gataaccgct aaccccgtaa tcactgaaag cactgagaac tctaagatga 2100
tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2160
agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2220
tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2280
gaggcgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2340
cattgtttgg aggaatgtcc tggttctcac aaatcctcat tggaacgttg ctgatgtggt 2400
tgggtctgaa cacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt 2460
tgatcttctt atccacagcc gtctctgctg atgtggggtg ctcggtggac ttctcaaaga 2520
aggagacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2580
ggtacaagta ccatcctgac tccccccgta gattggcagc agcagtcaag caagcctggg 2640
aagatggtat ctgcgggatc tcctctgttt caagaatgga gaacatcatg tggagatcag 2700
tagaagggga gctcaacgca atcttggaag agaatggagt tcaactgacg gtcgttgtgg 2760
gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2820
tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2880
acagctttgt cgtggatggt gacacactga aggaatgccc actcgaacat agagcatgga 2940
acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg 3000
ttagagaaga ttattcatta gagtgtgatc cagccgttat tggaacagct gttaagggga 3060
aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaat gacacatgga 3120
ggctgaagag ggcccatcta atcgagatga aaacatgtga atggccaaag tcccacacat 3180
tgtgggcaga tggaatagaa gagagtgatc tgatcattcc caagtcttta gctgggccac 3240
tcagccatca caataccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg 3300
aagagcttga aattcggttt gaggaatgcc cgggcactaa ggtccacgtg gaggaaacat 3360
gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3420
ggtgctgcag ggagtgcaca atgcccccac tgtcgttccg ggctaaagat ggctgttggt 3480
atggaatgga gataaggccc aggaaagaac cagaaagcaa cttagtaagg tcagtggtga 3540
ctgcaggatc aactgatcac atggatcact tctcccttgg agtgcttgtg attctgctca 3600
tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg 3660
cagtgctggt agctatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa 3720
ttttgatggg cgccaccttc gcggaaatga acactggagg agatgtagct catctggcgc 3780
tgatagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt 3840
ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct 3900
ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3960
tacgagcgat ggttgttcca cgcactgata acatcacctt ggcaatcctg gctgctctga 4020
caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4080
ggtttatgct cctctctctg aagggaaaag gcagtgtgaa gaagaactta ccatttgtca 4140
tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4200
tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc 4260
tgatatgcgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg 4320
ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4380
ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agtcactgga aacagtcccc 4440
ggctcgatgt ggcgctagat gagagtggtg atttctccct ggtggaggat gacggtcccc 4500
ccatgagaga gatcatactc aaggtggtcc tgatgaccat ctgtggcatg aacccaatag 4560
ccataccctt tgcagctgga gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg 4620
ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4680
acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag 4740
agggggtctt tcacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag 4800
ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat 4860
ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4920
gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca 4980
ttggagcggt tgcgctggat tacccagcag gaacttcagg atctccaatc ctagacaagt 5040
gtgggagagt gataggactt tatggcaatg gggtcgtgat caaaaatggg agttatgtta 5100
gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5160
tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga 5220
gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag 5280
ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt 5340
atatgacaac agcagtcaat gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc 5400
atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata 5460
ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5520
caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc 5580
gtgacgcatt tccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga 5640
gagcctggag ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg 5700
ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5760
tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt 5820
gggactttgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg 5880
tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattttgg 5940
ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga 6000
atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgacgaag 6060
accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc 6120
tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca 6180
agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg 6240
tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6300
ttgatggcac gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6360
gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6420
atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag 6480
tgatggaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg 6540
acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6600
cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6660
tgggaatctt tttcgtcttg atgaggaaca agggcatagg gaagatgggc tttggaatgg 6720
tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg 6780
catgtgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6840
aaagatctcc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg 6900
gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc 6960
taatgggaag gagagaggag ggagcaacca taggattctc aatggacatt gacctgcggc 7020
cagcctcagc ttgggccatc tatgctgcct tgacaacttt cattacccca gccgtccaac 7080
atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag 7140
tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7200
taatgatagg ttgctactca caattaacac ccctgaccct aatagtggcc atcattttgc 7260
tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc 7320
agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7380
acattgacac aatgacaatt gacccccaag tggagaaaaa gatgggacag gtgctactca 7440
tagcagtagc agtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg 7500
gggccctgat cacagccgca acttccactt tgtgggaagg ctctccgaac aagtactgga 7560
actcctctac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt 7620
ctctaatcta catagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag 7680
gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct 7740
actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca 7800
aggatggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt 7860
tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7920
ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa 7980
aaggaggccc tggtcatgaa gaacccgtgt tggtgcaaag ctatgggtgg aacatagtcc 8040
gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt 8100
gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8160
tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt 8220
gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8280
gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8340
cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8400
acgggcctag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8460
ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga 8520
tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8580
cttaccatgg aagctatgag gcccccacac aagggtcagc gtcctctcta ataaacgggg 8640
ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8700
ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc 8760
cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag 8820
agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8880
gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg 8940
aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga 9000
gaggagagtg ccagagttgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9060
aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc 9120
tagagttcga agcccttgga ttcttgaacg aggatcactg gatggggaga gagaactcag 9180
gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9240
gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca 9300
ggttcgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg catagggcct 9360
tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9420
ctgaaaaagg gaaaacagtt atggacatta tttcgagaca agaccaaagg gggagcggac 9480
aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata 9540
tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag 9600
tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag 9660
atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9720
atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9780
gggaagaagt tccgttttgc tcccaccact tcaacaagct ccatctcaag gacgggaggt 9840
ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgc gtctctccag 9900
gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc 9960
agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg 10020
tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10080
ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc 10140
acatggaaga caagacccca gttacgaaat ggacagacat tccctatttg ggaaaaaggg 10200
aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca 10260
ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact 10320
acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctgtaag 10380
caccaatctt aatgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10440
ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg 10500
gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10560
tgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10620
ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggagacc 10680
ccccggaaaa cgcaaaacag catattgacg ctgggaaaga ccagagactc catgagtttc 10740
caccacgctg gccgccaggc acagatcgcc gaatagcggc ggccggtgtg gggaaatcca 10800
tgggtctt 10808
<210> 7
<211> 10807
<212> DNA
<213> Zika病毒
<400> 7
agttgttgat ctgtgtgaat cagactgcga cagttcgagt ttgaagcgaa agctagcaac 60
agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 120
aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 180
gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca 240
ggatggtctt ggcgattcta gcctttttga gattcacggc aatcaagcca tcactgggtc 300
tcatcaatag atggggttca gtgggaaaaa aagaggctat ggaaataata aagaagttca 360
agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 420
gcacagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg 480
tcactagacg tgggagtgca tactatatgt acttggacag aagcgatgct ggggaggcca 540
tatcttttcc aaccacactg gggatgaata agtgttatat acagatcatg gatcttggac 600
acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtagaaccag 660
atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc 720
acaaaaaagg tgaagcacgg agatccagaa gagctgtgac gctcccctcc cattccacta 780
ggaagctgca aacgcggtcg cagacctggt tggaatcaag agaatacaca aagcacttga 840
ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg 900
cttggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga 960
ttgccccggc atacagcatc aggtgcatag gagtcagtaa tagggacttt gtggaaggta 1020
tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg 1080
cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1140
aggtaagatc ctactgctat gaggcatcaa tatcggacat ggcttcggac agccgctgcc 1200
caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa 1260
cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1320
catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1380
tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1440
acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1500
gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag 1560
gccttgactt ttcagatttg tattacttga ctatgaacaa caagcactgg ttggttcaca 1620
aggagtggtt ccacgacatt ccattacctt ggcacactgg ggcagacacc ggaactccac 1680
actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg 1740
tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1800
ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1860
tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca 1920
ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1980
cagatggacc ttgcaaggtt ccagctcaga tggcggtgga catgcaaact ctgaccccag 2040
ttgggaggtt gataaccgct aaccccgtaa tcactgaagg cactgagaac tctaagatga 2100
tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2160
agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2220
tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2280
gaggcgttct taactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2340
cattgtttgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctgatgtggt 2400
tgggtctgaa tacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt 2460
tgatcttctt atccacagcc gtctccgctg atgtggggtg ctcggtggac ttctcaaaga 2520
aggaaacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2580
ggtacaagta ccatcctgac tcccctcgta gattggcagc agtagtcaag caagcctggg 2640
aagatggtat ctgtgggatc tcctctgttt caagaatgga aaacatcatg tggagatcag 2700
tagaagggga gctcaacgca atcctggaag agaatggagt tcaactgacg gtcgttgtgg 2760
gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2820
tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2880
acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga 2940
acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg 3000
ttagagaaga ttattcacta gagtgtgatc cagccgtcat tggaacagct gttaagggaa 3060
aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaac gacacatgga 3120
ggctgaggag ggcccacctg atcgagatga aaacatgtga atggccaaag tcccacacat 3180
tgtggacaga tggaatagaa gagagtgatc tgatcatacc caagtcttta gctgggccac 3240
tcagccatca caacaccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg 3300
aagagcttga aattcggttt gaggaatgcc caggcactaa ggtccacgtg gaggaaacat 3360
gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3420
ggtgctgcag ggagtgcaca atgcccccac tgtcgttccg ggctaaagat ggctgttggt 3480
atggaatgga gataaggccc aggaaagaac cagaaagtaa cttagtaagg tcaatggtga 3540
ctgcaggatc aactgatcac atggatcact tttcccttgg agtgcttgtg attctgctca 3600
tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg 3660
cagtgctggt agctatgatc ctgggaggat tttcaatgag tgatctggct aagcttgcaa 3720
ttttgatggg tgccaccttt gcggaaatga acactggagg agatgtagct catctggcgc 3780
tggtagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt 3840
ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct 3900
ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3960
tacgagcgat ggttgttcca cgcactgaca atatcacctt ggcaatcctg gctgctctga 4020
caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4080
ggttcatgct cctctctctg aaggggaaag gcagtgtgaa gaagaactta ccatttgtca 4140
tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4200
tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc 4260
tgatatgcgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg 4320
ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4380
ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agttactgga aacagtcccc 4440
ggctcgatgt ggcactagat gagagtggtg atttctccct ggtggaggat gacggtcccc 4500
ccatgagaga gatcatactc aaagtggtcc tgatgaccat ctgtggcatg aacccaatag 4560
ccataccctt tgcagctgga gcgtggtacg tatacgtgaa aactggaaaa aggagtggtg 4620
ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4680
acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag 4740
agggggtctt tcacactatg tggcatgtca caaaaggatc cgcgctgaga agcggtgaag 4800
ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat 4860
ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4920
gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca 4980
ttggagcggt tgcgctggac tatccagcag gaacttcagg atctccaatc ctagacaagt 5040
gtgggagagt gataggactc tatggcaatg gggtcgtgat caagaatggg agttatgtca 5100
gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5160
tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga 5220
gagttcttcc tgaaatagtc cgtgaagcca taaaaacgag actccgtact gtgatcttag 5280
ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt 5340
atatgacaac agcagtcaat gtcacccatt ctgggacaga aatcgttgac ttaatgtgcc 5400
atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata 5460
ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5520
caagggttga gatgggcgag gcagctgcca tcttcatgac cgccacgcca ccaggaaccc 5580
gtgacgcatt cccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga 5640
gagcctggag ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg 5700
tcccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5760
tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt 5820
gggacttcgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg 5880
tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattctgg 5940
ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga 6000
atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgatgaag 6060
accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc 6120
tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca 6180
agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg 6240
tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6300
ttgatggcat gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6360
gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6420
atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag 6480
tgatggaagc cctgggaaca ctgccaggac acatgacgga gagattccag gaagccattg 6540
acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6600
cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6660
tgggaatctt tttcgtcttg atgcggaaca agggcatagg gaagatgggc tttggaatgg 6720
tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg 6780
catgcgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6840
aaagatcccc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg 6900
gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc 6960
taatgggaag gagagaggag ggggcaacca taggattctc aatggacatt gacctgcggc 7020
cagcctcggc ctgggccatc tatgctgccc tgacaacttt cattacccca gccgtccaac 7080
atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag 7140
tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7200
taatgatagg ttgctactca caattaacac ccctgaccct aatagtggct atcattttgc 7260
tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc 7320
agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7380
acattgacac aatgactatt gacccccaag tggagaaaaa gatgggacag gtgctactca 7440
tagcagtagc cgtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaagctg 7500
gggccctgat cacagctgca acttccactt tgtgggaagg ctctccgaac aagtactgga 7560
actcctctac agccacttca ctgtgcaaca tttttagggg aagttacttg gctggagctt 7620
ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag 7680
gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct 7740
actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca 7800
aggacggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt 7860
tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7920
ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa 7980
aaggaggccc tggtcatgaa gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc 8040
gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt 8100
gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8160
tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtgta aaagtgttgt 8220
gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8280
gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8340
cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8400
acgggcccag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8460
ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga 8520
tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8580
cttaccatgg aagctatgag gcccctacac aagggtcagc gtcctctcta ataaacgggg 8640
ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8700
ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac accagggtgc 8760
cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag 8820
agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8880
gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag accgcagtgg 8940
aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga 9000
gaggagagtg ccagagctgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9060
aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc 9120
tagagttcga agcccttgga ttcttaaatg aggatcactg gatggggaga gagaactcag 9180
gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9240
gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca 9300
ggtttgatct ggagaatgaa gctttaatca ccaaccaaat ggagaaaggg cacagggcct 9360
tagcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9420
ctgaaaaagg gaagacagtt atggacatta tttcaagaca agaccaaagg gggagcggac 9480
aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata 9540
tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag 9600
tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag 9660
atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9720
atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9780
gggaagaagt tccgttttgt tcccaccact tcaacaagct ccatctcaag gacgggaggt 9840
ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgt gtctctccag 9900
gggcgggatg gagcatccgg gagactgctt gcctagcaaa gtcatatgcg caaatgtggc 9960
agctccttta tttccacaga agggacctcc gactgatggc caatgccatc tgttcatctg 10020
tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10080
ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc 10140
acatggaaga caagacccca gttacgaaat ggacagacat tccctatctg ggaaaaaggg 10200
aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca 10260
ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact 10320
acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctataag 10380
caccaatctt agtgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10440
ctgtgacccc cccaggagag gctgggaaac caagcccata gtcaggccga gaacgccatg 10500
gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10560
cgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10620
ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggagacc 10680
ccccggaaaa cgcaaaacag catattgacg ctgggaaaga ccagagactc catgagtttc 10740
caccacgctg gccgccaggc acagatcgcc gaatagcggc ggccggtgtg gggaaatcca 10800
tgggtct 10807
<210> 8
<211> 10807
<212> DNA
<213> Zika病毒
<400> 8
agttgttgat ctgtgtgaat cagactgcga cagttcgagt ttgaagcgaa agctagcaac 60
agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 120
aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 180
gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggccat gggcccatca 240
ggatggtctt ggcgatacta gcctttttga gattcacggc aatcaagcca tcactgggtc 300
tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca 360
agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 420
gcgcagatac tagcgtcgga attgttggcc tcctcctgac cacagccatg gcagtagagg 480
tcactagacg tgggagtgca tactatatgt acttggacag aagcgatgct ggggaggcca 540
tatcttttcc aaccacactg gggatgaata agtgttacat acaaatcatg gatcttggac 600
acatgtgtga tgccaccatg agctatgaat gccctatgtt ggatgagggg gtagaaccag 660
atgacgtcga ttgctggtgc aacacgacat caacttgggt tgtgtatgga acctgccacc 720
acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta 780
ggaagctgca aacgcggtcg cagacctggt tggaatcaag agaatacaca aagcacctga 840
ttagagttga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgtcatcg 900
cttggctttt gggaagttca acgagccaaa aagtcatata tctggtcatg atactgctga 960
ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta 1020
tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtt accgtaatgg 1080
cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1140
aggtaagatc ctactgctat gaggcatcaa tatcggatat ggcttcggac agccgctgcc 1200
caacacaagg tgaggcctac cttgacaagc agtcagacac tcaatatgtc tgcaaaagaa 1260
cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1320
catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1380
tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1440
acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1500
gagccgaagc caccctgggg ggttttggga gcctaggact tgattgtgaa ccgaggacag 1560
gccttgactt ttcagatttg tattacctga ctatgaataa caagcactgg ttggttcaca 1620
aggagtggtt ccacgacatt ccattacctt ggcatgctgg ggcagacact ggaactccac 1680
attggaacaa caaagaagca ctggtagagt tcaaggacgc acatgcaaaa aggcaaactg 1740
tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1800
ctgagatgga tggagccaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1860
tggataaact tagattgaag ggcgtgtcat actccttgtg cactgcagcg ttcacattca 1920
ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1980
cagatggacc ttgcaaggtt ccagctcaga tggcggtgga tatgcaaact ctgaccccag 2040
ttgggaggtt gataaccgct aaccctgtaa tcactgaaag caccgagaac tctaagatga 2100
tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2160
agatcaccca tcactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2220
tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2280
ggggtgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2340
cattgttcgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctggtgtggt 2400
tgggtctgaa tacaaagaat ggatctattt cccttacgtg cttggcctta gggggagtgt 2460
tgatcttctt atccacagcc gtttctgctg atgtggggtg ctcggtggac ttctcaaaga 2520
aggaaacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2580
ggtacaagta ccatcctgac tcccctcgta gattggcagc agcagtcaag caagcctggg 2640
aagatgggat ctgtgggatc tcctctgtct caagaatgga aaacatcatg tggagatcag 2700
tagaagggga gctcaacgca atcctggaag agaatggagt tcaactgacg gtcgttgtgg 2760
gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2820
tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2880
acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga 2940
acagctttct tgtggaggat catgggtttg gggtatttca cactagtgtc tggctcaagg 3000
ttagagaaga ttattcatta gagtgtgatc cagccgtcat tggaacagct gctaagggaa 3060
aggaggctgt gcacagcgat ctaggctact ggattgagag tgagaagaac gacacatgga 3120
ggctgaagag ggcccacctg atcgagatga aaacatgtga atggccaaag tcccacacat 3180
tgtggacaga tggagtagaa gaaagtgatc tgatcatacc caagtcttta gctgggccac 3240
tcagccatca caacaccaga gagggctaca ggactcaaat gaaagggcca tggcacagtg 3300
aagagcttga aattcggttt gaggaatgcc caggcactaa ggtccacgtg gaggaaacat 3360
gtgggacaag aggaccatcc ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3420
ggtgctgcag ggaatgcaca atgcccccac tgtcgttccg agctaaagat ggctgttggt 3480
atggaatgga gataaggccc aggaaagaac cagaaagtaa cttagtaagg tcaatggtga 3540
ctgcaggatc aactgatcac atggatcact tctctcttgg agtgcttgtg attttgctca 3600
tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcaatgg 3660
cagtgctggt agccatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa 3720
ttttgatggg tgccaccttc gcggaaatga acactggagg agatgtagct catttggcgc 3780
tgatagcggc attcaaagtc agacctgcgt tgctggtatc tttcatcttc agagctaatt 3840
ggacaccccg tgagagcatg ctgctggcct tggcctcgtg tcttctgcaa actgcgatct 3900
ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3960
tacgagcgat ggttgttcca cgcactgaca acatcacctt ggcaatcctg gctgctctga 4020
caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4080
ggttcatgct cctctctctg aaggggaaag gcagtgtgaa gaagaaccta ccatttgtca 4140
tggccttggg actaactgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4200
tgctcacaag gagtgggaag cggagctggc cccctagtga agtactcaca gctgttggcc 4260
tgatatgcgc attggctgga gggttcgcca aggcggatat agagatggct gggcccatgg 4320
ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4380
ttgaaagagc aggtgacatc acatgggaaa aagatgcgga aatcactgga aacagtcccc 4440
ggctcgatgt ggcactagat gagagtggtg atttctccct agtggaggat gatggtccac 4500
ccatgagaga gatcatactc aaagtggtcc tgatgaccat ctgcggcatg aacccaatag 4560
ccataccctt tgcagctgga gcgtggtacg tgtatgtgaa gactggaaaa aggagtggtg 4620
ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4680
acagagtaat gactcgtaga ctgcttggtt caacacaagt tggagtggga gtcatgcaag 4740
agggggtctt ccacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag 4800
ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccgt 4860
ggaagctaga cgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4920
gagagagagc gaggaacatc cagactctgc ccggaacatt taagacaaag gatggggaca 4980
ttggagcagt tgcgctggac tacccagcag gaacttcagg atctccaatc ctagacaagt 5040
gtgggagagt gataggactc tatggtaatg gggtcgtgat aaaaaatggg agttatgtta 5100
gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5160
tgctgaagaa gaagcagcta actgtcttag acctgcatcc tggagccggg aaaaccagga 5220
gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag 5280
ctccaaccag ggtcgtcgct gctgaaatgg aggaagccct tagagggctt ccagttcgtt 5340
atatgacaac agcagtcaat gtcacccatt ctgggacaga aatcgttgac ttaatgtgcc 5400
atgctacctt cacttcacgc ctactacaac caatcagagt ccccaactat aatttgtata 5460
ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5520
caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc 5580
gtgacgcatt cccggactcc aactcaccaa ttatggacac cgaggtggaa gtcccagaga 5640
gagcctggag cacaggcttt gattgggtga cggatcattc tgggaaaaca gtctggtttg 5700
ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5760
tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacgaaa aatcaagagt 5820
gggacttcgt cgtgacaacc gacatttcag agatgggcgc caactttaaa gctgaccgtg 5880
tcatagattc caggagatgc ttaaagccgg tcatacttga tggcgagaga gtcattttgg 5940
ctggacccat gcctgtcaca catgccagcg ctgctcagag gagggggcgc ataggcagga 6000
atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgatgaag 6060
atcacgcaca ctggcttgaa gcaagaatgc ttcttgacaa catttacctc caagatggcc 6120
tcatagcttc gctctatcga cctgaggccg acaaagtagc agctattgag ggagagttca 6180
agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttccgg 6240
tttggttggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6300
ttgatggcat gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6360
gatacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6420
atgcggccct gaagtcattc aaagagtttg ccgctgggaa aagaggagcg gcctttggag 6480
tgatagaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg 6540
acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6600
cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6660
tgggaatctt tttcgtcttg atgcggaaca agggcatggg gaagatgggc tttggaatgg 6720
tgactcttgg ggccagcgca tggcttatgt ggctctcgga aattgagcca gccagaattg 6780
catgtgtcct cattgtcgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6840
aaagatctcc tcaggacaac caaatggcaa tcatcatcat ggtagcagtg ggtcttctgg 6900
gcttgattac cgccaatgaa ctcggatggt tggagagaac aaaaagtgac ctaagccatc 6960
taatgggaag gagagaggag ggggcaacca caggattctc aatggacatt gacctgcggc 7020
cagcctcagc ttgggctatc tatgctgctc tgacaacttt catcacccca gccgtccaac 7080
atgcggtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctgggg 7140
tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7200
taatgatggg ttgctactca caattaacac ctctgaccct aatagtggcc atcattttgc 7260
tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgggctgccc 7320
agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7380
acattgacac aatgacaatt gacccccaag tggaaaaaaa gatggggcag gtgctactca 7440
tagcagtagc cgtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg 7500
gggccctgat cacagctgca acttccacct tgtgggaagg ctctccgaac aagtactgga 7560
actcctccac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt 7620
ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacgg 7680
gagagaccct gggagagaaa tggaaggccc gcctgaacca gatgtcggcc ctggagttct 7740
actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgtgccctca 7800
aggacggtgt ggcaacagga ggccatgctg tgtcccgagg aagtgcaaag cttagatggc 7860
tggtggagag aggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7920
ggggctggag ttactatgcc gccaccatcc gcaaagttca ggaagtgaaa ggatacacaa 7980
aaggaggccc tggtcatgaa gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc 8040
gtcttaagag tggggtggac gtctttcaca tggcggctga gccgtgtgac actttgctgt 8100
gtgatatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8160
tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt 8220
gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8280
gactggtcag ggtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8340
cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8400
acgggcccag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8460
ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgagagga 8520
tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8580
cttaccatgg aagctatgag gcccctacac aagggtcagc gtcctctcta ataaacgggg 8640
ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8700
ctgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc 8760
cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttatggaagg 8820
agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8880
gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg 8940
aagctgtgaa tgatccaagg ttctgggctc tagtggacaa ggaaagagag catcacctga 9000
gaggagagtg tcagagctgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9060
aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagattcc 9120
tagagttcga agcccttgga ttcttgaatg aggatcattg gatggggaga gagaattcag 9180
gaggtggtgt tgaaggactg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9240
gcataccagg aggaaggatg tatgcagatg atactgctgg ctgggacacc cgcatcagca 9300
ggtttgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg cacagggcct 9360
tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9420
ctgaaaaagg gaagacagtt atggacatta tttcaagaca agaccaaagg gggagcggac 9480
aagttgtcac ttacgctctt aatacattca ccaacctggt ggtgcagctc attcggaata 9540
tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg ccagagaaag 9600
tgaccaactg gttgcaaagc aacggatggg ataggctcaa aagaatggca gtcagtggag 9660
atgattgcgt tgtgaaacca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9720
atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9780
gggaagaagt tccgttttgc tcccaccact tcaacaaact ccatcttaag gacgggaggt 9840
ccattgtggt tccctgccgc caccaagatg aactgattgg ccgagcccgc gtatcaccag 9900
gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc 9960
agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg 10020
tgccagttga ttgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10080
ggatgaccac tgaagacatg cttgtggtat ggaacagagt gtggattgag gaaaacgacc 10140
acatggaaga caagacccca gttacaaaat ggacagacat tccctatttg ggaaaaagag 10200
aagacttgtg gtgtggatct ctcatagggc acagaccgcg tactacctgg gctgagaaca 10260
tcaaaaatac agtcaacatg atgcgcagga tcataggtga tgaagaaaag tacatggact 10320
acctatccac ccaggttcgc tacttgggtg aagaagggtc cacacctgga gtgctgtaag 10380
caccaatctt agtgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10440
ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg 10500
gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10560
cgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10620
ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggagacc 10680
ccccggaaaa cgcaaaacag catattgacg ctgggaaaga ccagagactc catgagtttc 10740
caccacgctg gccgccaggc acagatcgcc gaatagcggc ggccggtgtg gggaaatcca 10800
tgggtct 10807
<210> 9
<211> 10648
<212> DNA
<213> Zika病毒
<400> 9
gacagttcga gtttgaagcg aaagctagca acagtatcaa caggttttat ttggatttgg 60
aaacgagagt ttctggtcat gaaaaaccca aaaaagaaat ccggaggatt ccggattgtc 120
aatatgctaa aacgcggagt agcccgtgtg agcccctttg ggggcttgaa gaggctgcca 180
gccggacttc tgctgggtca tgggcccatc aggatggtct tggcgattct agcctttttg 240
agattcacgg caatcaagcc atcactgggt ctcatcaata gatggggttc agtggggaaa 300
aaagaggcta tggaaataat aaagaagttc aagaaagatc tggctgccat gctgagaata 360
atcaatgcta ggaaggagaa gaagagacga ggcgcagata ctagtgtcgg aattgttggc 420
ctcctgctga ccacagctat ggcagcggag gtcactagac gtgggagtgc atactatatg 480
tacttggaca gaaacgatgc tggggaggcc atatcttttc caaccacatt ggggatgaat 540
aagtgttata tacagatcat ggatcttgga cacatgtgtg atgccaccat gagctatgaa 600
tgccctatgc tggatgaggg ggtggaacca gatgacgtcg attgttggtg caacacgacg 660
tcaacttggg ttgtgtacgg aacctgccat cacaaaaaag gtgaagcacg gagatctaga 720
agagctgtga cgctcccctc ccattccact aggaagctgc aaacgcggtc gcaaacctgg 780
ttggaatcaa gagaatacac aaagcacttg attagagtcg aaaattggat attcaggaac 840
cctggcttcg cgttagcagc agctgccatc gcttggcttt tgggaagctc aacgagccaa 900
aaagtcatat acttggtcat gatactgctg attgccccgg catacagcat caggtgcata 960
ggagtcagca atagggactt tgtggaaggt atgtcaggtg ggacctgggt tgatgttgtc 1020
ttggaacatg gaggttgtgt caccgtaatg gcacaggaca aaccgactgt cgacatagag 1080
ctggttacaa caacagtcag caacatggcg gaggtaagat cctactgcta tgaggcatca 1140
atatcagaca tggcttcgga cagccgctgc ccaacacaag gtgaagccta ccttgacaag 1200
caatcagaca ctcaatatgt ctgcaaaaga acgttagtgg acagaggctg gggaaatgga 1260
tgtggacttt ttggcaaagg gagcctggtg acatgcgcta agtttgcatg ctccaagaaa 1320
atgaccggga agagcatcca gccagagaat ctggagtacc ggataatgct gtcagttcat 1380
ggctcccagc acagtgggat gattgttaat gacacaggac atgaaactga tgagaataga 1440
gcgaaagttg agataacgcc caattcacca agagccgaag ccaccctggg gggttttgga 1500
agcctaggac ttgattgtga accgaggaca ggccttgact tttcagattt gtattacttg 1560
actatgaata acaagcactg gttggttcac aaggagtggt tccacgacat tccattacct 1620
tggcacgctg gggcagacac cggaactcca cactggaaca acaaagaagc actggtagag 1680
ttcaaggacg cacatgccaa aaggcaaact gtcgtggttc tagggagtca agaaggagca 1740
gttcacacgg cccttgctgg agctctggag gctgagatgg atggtgcaaa gggaaggctg 1800
tcctctggcc acttgaaatg tcgcctgaaa atggataaac ttagattgaa gggcgtgtca 1860
tactccttgt gtactgcagc gttcacattc accaagatcc cggctgaaac actgcacggg 1920
acagtcacag tggaggtaca gtacgcaggg acagatggac cttgcaaggt tccagctcag 1980
atggcggtgg acatgcaaac tctgacccca gttgggaggt tgataaccgc taaccccgta 2040
atcactgaaa gcactgagaa ctctaagatg atgctggaac ttgatccacc atttggggac 2100
tcttacattg tcataggagt cggggagaag aagatcaccc accactggca caggagtggc 2160
agcaccattg gaaaagcatt tgaagccact gtgagaggtg ccaagagaat ggcagtcttg 2220
ggagacacag cctgggactt tggatcagtt ggaggcgctc tcaactcatt gggcaagggc 2280
atccatcaaa tttttggagc agctttcaaa tcattgtttg gaggaatgtc ctggttctca 2340
caaattctca ttggaacgtt gctgatgtgg ttgggtctga acacaaagaa tggatctatt 2400
tcccttatgt gcttggcctt agggggagtg ttgatcttct tatccacagc cgtctctgct 2460
gatgtggggt gctcggtgga cttctcaaag aaggagacga gatgcggtac aggggtgttc 2520
gtctataacg acgttgaagc ctggagggac aggtacaagt accatcctga ctccccccgt 2580
agattggcag cagcagtcaa gcaagcctgg gaagatggta tctgcgggat ctcctctgtt 2640
tcaagaatgg aaaacatcat gtggagatca gtagaagggg agctcaacgc aatcctggaa 2700
gagaatggag ttcaactgac ggtcgttgtg ggatctgtaa aaaaccccat gtggagaggt 2760
ccacagagat tgcccgtgcc tgtgaacgag ctgccccacg gctggaaggc ttgggggaaa 2820
tcgtacttcg tcagagcagc aaagacaaat aacagctttg tcgtggatgg tgacacactg 2880
aaggaatgcc cactcaaaca tagagcatgg aacagctttc ttgtggagga tcatgggttc 2940
ggggtatttc acactagtgt ctggctcaag gttagagaag attattcatt agagtgtgat 3000
ccagccgtta ttggaacagc tgttaaggga aaggaggctg tacacagtga tctaggctac 3060
tggattgaga gtgagaagaa tgacacatgg aggctgaaga gggcccatct gatcgagatg 3120
aaaacatgtg aatggccaaa gtcccacaca ttgtggacag atggaataga agagagtgat 3180
ctgatcatac ccaagtcttt agctgggcca ctcagccatc acaataccag agagggctac 3240
aggacccaaa tgaaagggcc atggcacagt gaagagcttg aaattcggtt tgaggaatgc 3300
ccaggcacta aggtccacgt ggaggaaaca tgtggaacaa gaggaccatc tctgagatca 3360
accactgcaa gcggaagggt gatcgaggaa tggtgctgca gggagtgcac aatgccccca 3420
ctgtcgttcc gggctaaaga tggctgttgg tatggaatgg agataaggcc caggaaagaa 3480
ccagaaagca acttagtaag gtcaatggtg actgcaggat caactgatca catggaccac 3540
ttctcccttg gagtgcttgt gattctgctc atggtgcagg aagggctgaa gaagagaatg 3600
accacaaaga tcatcataag cacatcaatg gcagtgctgg tagctatgat cctgggagga 3660
ttttcaatga gtgacctggc taagcttgca attttgatgg gtgccacctt cgcggaaatg 3720
aacactggag gagatgtagc tcatctggcg ctgatagcgg cattcaaagt cagaccagcg 3780
ttgctggtat ctttcatctt cagagctaat tggacacccc gtgaaagcat gctgctggcc 3840
ttggcctcgt gtcttttgca aactgcgatc tccgccttgg aaggcgacct gatggttctc 3900
atcaatggtt ttgctttggc ctggttggca atacgagcga tggttgttcc acgcactgat 3960
aacatcacct tggcaatcct ggctgctctg acaccactgg cccggggcac actgcttgtg 4020
gcgtggagag caggccttgc tacttgcggg gggtttatgc tcctctctct gaagggaaaa 4080
ggcagtgtga agaagaactt accatttgtc atggccctgg gactaaccgc tgtgaggctg 4140
gtcgacccca tcaacgtggt gggactgctg ttgctcacaa ggagtgggaa gcggagctgg 4200
ccccctagcg aagtactcac agctgttggc ctgatatgcg cattggctgg agggttcgcc 4260
aaggcagata tagagatggc tgggcccatg gccgcggtcg gtctgctaat tgtcagttac 4320
gtggtctcag gaaagagtgt ggacatgtac attgaaagag caggtgacat cacatgggaa 4380
aaagatgcgg aagtcactgg aaacagtccc cggctcgatg tggcgctaga tgagagtggt 4440
gatttctccc tggtggagga tgacggtccc cccatgagag agatcatact caaggtggtc 4500
ctgatgacca tctgtggcat gaacccaata gccataccct ttgcagctgg agcgtggtac 4560
gtatacgtga agactggaaa aaggagtggt gctctatggg atgtgcctgc tcccaaggaa 4620
gtaaaaaagg gggagaccac agatggagtg tacagagtaa tgactcgtag actgctaggt 4680
tcaacacaag ttggagtggg agttatgcaa gagggggtct ttcacactat gtggcacgtc 4740
acaaaaggat ccgcgctgag aagcggtgaa gggagacttg atccatactg gggagatgtc 4800
aagcaggatc tggtgtcata ctgtggtcca tggaagctag atgccgcctg ggacgggcac 4860
agcgaggtgc agctcttggc cgtgcccccc ggagagagag cgaggaacat ccagactctg 4920
cccggaatat ttaagacaaa ggatggggac attggagcgg ttgcgctgga ttacccagca 4980
ggaacttcag gatctccaat cctagacaag tgtgggagag tgataggact ttatggcaat 5040
ggggtcgtga tcaaaaatgg gagttatgtt agtgccatca cccaagggag gagggaggaa 5100
gagactcctg ttgagtgctt cgagccttcg atgctgaaga agaagcagct aactgtctta 5160
gacttgcatc ctggagctgg gaaaaccagg agagttcttc ctgaaatagt ccgtgaagcc 5220
ataaaaacaa gactccgtac tgtgatctta gctccaacca gggttgtcgc tgctgaaatg 5280
gaggaggccc ttagagggct tccagtgcgt tatatgacaa cagcagtcaa tgtcacccac 5340
tctggaacag aaatcgtcga cttaatgtgc catgccacct tcacttcacg tctactacag 5400
ccaatcagag tccccaacta taatctgtat attatggatg aggcccactt cacagatccc 5460
tcaagtatag cagcaagagg atacatttca acaagggttg agatgggcga ggcggctgcc 5520
atcttcatga ccgccacgcc accaggaacc cgtgacgcat ttccggactc caactcacca 5580
attatggaca ccgaagtgga agtcccagag agagcctgga gctcaggctt tgattgggtg 5640
acggatcatt ctggaaaaac agtttggttt gttccaagcg tgaggaacgg caatgagatc 5700
gcagcttgtc tgacaaaggc tggaaaacgg gtcatacagc tcagcagaaa gacttttgag 5760
acagagttcc agaaaacaaa acatcaagag tgggactttg tcgtgacaac tgacatttca 5820
gagatgggcg ccaactttaa agctgaccgt gtcatagatt ccaggagatg cctaaagccg 5880
gtcatacttg atggcgagag agtcattctg gctggaccca tgcctgtcac acatgccagc 5940
gctgcccaga ggagggggcg cataggcagg aatcccaaca aacctggaga tgagtatctg 6000
tatggaggtg ggtgcgcaga gactgacgaa gaccatgcac actggcttga agcaagaatg 6060
ctccttgaca atatttacct ccaagatggc ctcatagcct cgctctatcg acctgaggcc 6120
gacaaagtag cagccattga gggagagttc aagcttagga cggagcaaag gaagaccttt 6180
gtggaactca tgaaaagagg agatcttcct gtttggctgg cctatcaggt tgcatctgcc 6240
ggaataacct acacagatag aagatggtgc tttgatggca cgaccaacaa caccataatg 6300
gaagacagtg tgccggcaga ggtgtggacc agacacggag agaaaagagt gctcaaaccg 6360
aggtggatgg acgccagagt ttgttcagat catgcggccc tgaagtcatt caaggagttt 6420
gccgctggga aaagaggagc ggcttttgga gtgatggaag ccctgggaac actgccagga 6480
cacatgacag agagattcca ggaagccatt gacaacctcg ctgtgctcat gcgggcagag 6540
actggaagca ggccttacaa agccgcggcg gcccaattgc cggagaccct agagaccatt 6600
atgcttttgg ggttgctggg aacagtctcg ctgggaatct tcttcgtctt gatgaggaac 6660
aagggcatag ggaagatggg ctttggaatg gtgactcttg gggccagcgc atggctcatg 6720
tggctctcgg aaattgagcc agccagaatt gcatgtgtcc tcattgttgt gtttctattg 6780
ctggtggtgc tcatacctga gccagaaaag caaagatctc cccaggacaa ccaaatggca 6840
atcatcatca tggtagcagt aggtcttctg ggcttgatta ccgccaatga actcggatgg 6900
ttggagagaa caaagagtga cctaagccat ctaatgggaa ggagagagga gggggcaacc 6960
ataggattct caatggacat tgacctgcgg ccagcctcag cttgggccat ctatgctgcc 7020
ttgacaactt tcattacccc agccgtccaa catgcagtga ccacttcata caacaactac 7080
tccttaatgg cgatggccac gcaagctgga gtgttgtttg gtatgggcaa agggatgcca 7140
ttctacgcat gggactttgg agtcccgctg ctaatgatag gttgctactc acaattaaca 7200
cccctgaccc taatagtggc catcattttg ctcgtggcgc actacatgta cttgatccca 7260
gggctgcagg cagcagctgc gcgtgctgcc cagaagagaa cggcagctgg catcatgaag 7320
aaccctgttg tggatggaat agtggtgact gacattgaca caatgacaat tgacccccaa 7380
gtggagaaaa agatgggaca ggtgctactc atagcagtag ccgtctccag cgccatactg 7440
tcgcggaccg cctgggggtg gggggaggct ggggccctga tcacagccgc aacttccact 7500
ttgtgggaag gctctccgaa caagtactgg aactcctcta cagccacttc actgtgtaac 7560
atttttaggg gaagttactt ggctggagct tctctaatct acacagtaac aagaaacgct 7620
ggcttggtca agagacgtgg gggtggaaca ggagagaccc tgggagagaa atggaaggcc 7680
cgcttgaacc agatgtcggc cctggagttc tactcctaca aaaagtcagg catcaccgag 7740
gtgtgcagag aagaggcccg ccgcgccctc aaggacggtg tggcaacggg aggccatgct 7800
gtgtcccgag gaagtgcaaa gctgagatgg ttggtggagc ggggatacct gcagccctat 7860
ggaaaggtca ttgatcttgg atgtggcaga gggggctgga gttactacgc cgccaccatc 7920
cgcaaagttc aagaagtgaa aggatacaca aaaggaggcc ctggtcatga agaacccgtg 7980
ttggtgcaaa gctatgggtg gaacatagtc cgtcttaaga gtggggtgga cgtctttcat 8040
atggcggctg agccgtgtga cacgttgctg tgtgacatag gtgagtcatc atctagtcct 8100
gaagtggaag aagcacggac gctcagagtc ctctccatgg tgggggattg gcttgaaaaa 8160
agaccaggag ccttttgtat aaaggtgttg tgcccataca ccagcactat gatggaaacc 8220
ctggagcgac tgcagcgtag gtatggggga ggactggtca gagtgccact ctcccgcaac 8280
tctacacatg agatgtattg ggtctctgga gcgaaaagca acaccataaa aagtgtgtcc 8340
accacgagcc agctcctctt ggggcgcatg gacgggccta ggaggccagt gaaatatgag 8400
gaggatgtga atctcggctc tggcacgcgg gctgtggtaa gctgcgctga agctcccaac 8460
atgaagatca ttggtaaccg cattgaaagg atccgcagtg agcacgcgga aacgtggttc 8520
tttgacgaga accacccata taggacatgg gcttaccatg gaagctatga ggcccccaca 8580
caagggtcag cgtcctctct aataaacggg gttgtcaggc tcctgtcaaa accctgggat 8640
gtggtgactg gagtcacagg aatagccatg accgacacca caccgtatgg tcagcaaaga 8700
gttttcaagg aaaaagtgga cactagggtg ccagaccccc aagaaggcac tcgtcaggtt 8760
atgagcatgg tctcttcctg gttgtggaaa gagctaggca aacacaaacg gccacgagtc 8820
tgtaccaaag aagagttcat caacaaggtt cgtagcaatg cagcattagg ggcaatattt 8880
gaagaggaaa aagagtggaa gactgcagtg gaagctgtga acgatccaag gttctgggct 8940
ctagtggata aggaaagaga gcaccacctg agaggagagt gccagagttg tgtgtacaac 9000
atgatgggaa aaagagaaaa gaaacaaggg gaatttggaa aggccaaggg cagccgcgcc 9060
atctggtata tgtggctagg ggctagattt ctagagttcg aagcccttgg attcttgaac 9120
gaggatcact ggatggggag agagaactca ggaggtggtg ttgaagggct gggattacaa 9180
agactcggat atgtcctaga agagatgagt cgtataccag gaggaaggat gtatgcagat 9240
gacactgctg gctgggacac ccgcatcagc aggtttgatc tggagaatga agctctaatc 9300
accaaccaaa tggaaaaagg gcacagggcc ttggcattgg ccataatcaa gtacacatac 9360
caaaacaaag tggtaaaggt ccttagacca gctgaaaaag ggaaaacagt tatggacatt 9420
atttcgagac aagaccaaag ggggagcgga caagttgtca cttacgctct taacacattt 9480
accaacctag tggtgcaact cattcggaat atggaggctg aggaagttct agagatgcaa 9540
gacttgtggc tgctgcggag gtcagagaaa gtgaccaact ggttgcagag caacggatgg 9600
gataggctca aacgaatggc agtcagtgga gatgattgcg ttgtgaagcc aattgatgat 9660
aggtttgcac atgccctcag gttcttgaat gatatgggaa aagttaggaa ggacacacaa 9720
gagtggaaac cctcaactgg atgggacaac tgggaagaag ttccgttttg ctcccaccac 9780
ttcaacaagc tccatctcaa ggacgggagg tccattgtgg ttccctgccg ccaccaagat 9840
gaactgattg gccgggcccg cgtctctcca ggggcgggat ggagcatccg ggagactgct 9900
tgcctagcaa aatcatatgc gcaaatgtgg cagctccttt atttccacag aagggacctc 9960
cgactgatgg ccaatgccat ttgttcatct gtgccagttg actgggttcc aactgggaga 10020
actacctggt caatccatgg aaagggagaa tggatgacca ctgaagacat gcttgtggtg 10080
tggaacagag tgtggattga ggagaacgac cacatggaag acaagacccc agttacgaaa 10140
tggacagaca tcccctattt gggaaaaagg gaagacttgt ggtgtggatc tctcataggg 10200
cacagaccgc gcaccacctg ggctgagaac attaaaaaca cagtcaacat ggtgcgcagg 10260
atcataggtg atgaagaaaa gtacatggac tacctatcca cccaagttcg ctacttgggt 10320
gaagaagggt ctacacctgg agtgctgtaa gcaccagtct taatgttgtc aggcctgcta 10380
gtcagccaca gcttggggaa agctgtgcag cctgtgaccc ccccaggaga agctgggaaa 10440
ccaagcctat agtcaggccg agaacgccat ggcacggaag aagccatgct gcctgtgagc 10500
ccctcagagg acactgagtc aaaaaacccc acgcgcttgg aggcgcagga tgggaaaaga 10560
aggtggcgac cttccccacc cttcaatctg gggcctgaac tggagatcag ctgtggatct 10620
ccagaagagg gactagtggt tagaggag 10648
<210> 10
<211> 10676
<212> DNA
<213> Zika病毒
<400> 10
gttgttactg ttgctgactc agactgcgac agttcgagtt tgaagcgaaa gctagcaaca 60
gtatcaacag gttttatttg gatttggaaa cgagagtttc tggtcatgaa aaacccaaaa 120
aagaaatccg gaggattccg gattgtcaat atgctaaaac gcggagtagc ccgtgtgagc 180
ccctttgggg gcttgaagag gctgccagcc ggacttctgc tgggtcatgg gcccatcagg 240
atggtcttgg caattctagc ctttttgaga ttcacggcaa tcaagccatc actgggtctc 300
atcaatagat ggggttcagt ggggaaaaaa gatgctatgg aaataataaa gaagttcaag 360
aaagatctgg ctgccatgct gagaataatc aatgctagga aggagaagaa gagacgaggc 420
gcagatacta gtgtcggaat tgttggcctc ctgctgacca cagctatggc agcggaggtc 480
actagacgtg ggagtgcata ctatatgtac ttggacagaa acgatgctgg ggaggccata 540
tcttttccaa ccacattggg gatgaataag tgttatatac agatcatgga tcttggacac 600
atgtgtgatg ccaccatgag ctatgaatgc cctatgctgg atgagggggt ggaaccagat 660
gacgtcgatt gttggtgcaa cacgacgtca acttgggttg tgtacggaac ctgccatcac 720
aaaaaaggtg aagcacggag atctagaaga gctgtgacgc tcccttccca ttccactagg 780
aagctgcaaa cgcggtcgca aacctggttg gaatcaagag aatacacaaa gcacttgatt 840
agagtcgaaa attggatatt caggaaccct ggcttcgcgt tagcagcagc tgccatcgct 900
tggcttttgg gaagctcaac gagccaaaaa gtcatatact tggtcatgat actgctgatt 960
gccccggcat acagcatcag gtgcatagga gtcagcaata gggactttgt ggaaggtatg 1020
tcaggtggga cttgggttga tgttgtcttg gaacatggag gttgtgtcac cgcaatggca 1080
caggacaaac cgactgtcga catagagctg gttacaacaa cagtcagcaa catggcggag 1140
gtaagatcct actgctatga ggcatcaata tcagacatgg cttcggacag ccgctgccca 1200
acacaaggtg aagcctacct tgacaagcaa tcagacactc aatatgtttg caaaagaacg 1260
ttagtggaca gaggctgggg aaatggatgt ggactttttg gcaaagggag tctggtgaca 1320
tgcgctaagt ttgcatgctc caagaaaatg accgggaaga gcatccagcc agagaatctg 1380
gagtaccgga taatgctgtc agttcatggc tcccagcaca gtgggatgct cgttaatgac 1440
acaggacatg aaactgatga gaatagagcg aaggttgaga taacgcccaa ttcaccaaga 1500
gccgaagcca ccctgggggg ttttggaagc ctaggacttg attgtgaacc gaggacaggc 1560
cttgactttt cagatttgta ttacttgact atgaataaca agcactggtt ggctcacaag 1620
gagtggttcc acgacattcc attaccttgg cacgctgggg cagccaccgg aactccacac 1680
tggaacaaca aagaagcact ggtagagttc aaggacgcac atgccaaaag gcaaactgtc 1740
gtggttctag ggagtcaaga aggagcagtt cacacggccc ttgctggagc tctggaggct 1800
gagatggatg gtgcaaaggg aaggctgtcc tctggccact tgaaatgtcg cctgaaaatg 1860
gataaactta gattgaaggg cgtgtcatac tccttgtgta ccgcagcgtt cacattcacc 1920
aagatcccgg ctgaaacagt ggacgggaca gtcacagtgg agggacagta cggagggaca 1980
gatggacctt gcaaggttcc agctcagatg gcggtggaca tgcagactct gaccccagtt 2040
gggaggttga taaccgctaa ccccgtaatc actgaaagca ctgagaactc taagatgatg 2100
ctggaacttg atccaccatt tggggactct tacattgtca taggagtcgg ggagaagaag 2160
atcacccacc actggcacag gagtggcagc accattggaa aagcatttga agccactgtg 2220
agaggtgcca agagaatggc agtcttggga gacacagcct gggactttgg atcagttgga 2280
ggcgctctca actcattggg caagggcatc catcaaatta ttggagcagc tttcaaatca 2340
ttgtttggag gaatgtcctg gttctcacaa attctcattg ggacgttgct gatgtggttg 2400
ggtctgaaca caaagaatgg atctatttcc cttatgtgct tggccttagg gggagtgttg 2460
atcttcttat ccacagccgt ctcaggtggt gtggggtgct cggtggactt ctcaaagaag 2520
gagacgagat gcggtacagg ggtgttcgtc tataacgatg ttgaagcctg gagggacagg 2580
tacaagtacc atcctgactc cccccgtaga ttggcagcag cagtcaagca agcctgggaa 2640
gatggtatct gcgggatctc ctctgtttca agaatggaaa acatcatgtg gagatcagta 2700
gaaggggagc tcaacgcaat cctggaagag aatggagttc aactgacggt cgttgtggga 2760
tctgtaaaaa accccatgtg gagaggtcca cagagattgc ccgtgcctgt gaacgagctg 2820
ccccacggct ggaaggcttg ggggaaatcg tacttcgtca gagcagcaaa gacaaataac 2880
agctttgtcg tggatggtga cacactgaag gaatgcccac tcaaacatag agcatggaac 2940
agctttcttg tggaggatca tgggttcggg gtatttcaca ctagtgtctg gctcaaggtt 3000
agagaagact attggttaga gtgtgatcca gccgttattg gaacagctgt taagggaaag 3060
gaggctgtac acagtgatct aggctactgg attgagagtg agaagaatga cacatggtgg 3120
ctgaagaggg cccatctgat cgagatgaaa acatgtgaat ggccaaagtc ccacacattg 3180
tggacagatg gaatagaaga gagtgatctg atcataccca agtctttagc tgggccactc 3240
agccatcaca atgccagaga gggctacagg acccaaatga aagggccatg gcacagtgaa 3300
gagcttgaaa ttcggtttga ggaatgccca ggcactaagg tccacgtgga ggaaacatgt 3360
ggaacaagag gaccatctct gagatcaacc actgcaagcg gaagggtgat cgaggaatgg 3420
tgctccaggg agtgcacaat gcccccactg tccttccagg ctaaagatgg ctgttggtat 3480
ggaatggaga taaggcccag gaaagaacca gaaagcaact tagtaaggtc aatggtgact 3540
gcaggatcaa ctgatcacat ggatcacttc tcccttggag tgcttgtgat tctgctcatg 3600
gtgcaggaag ggctgaagaa gagaatgacc acaaagatca tcataagcac atcaatggca 3660
gtgctggtag ctatgatcct gggaggattt tcaatgagtg acctggctaa gcttgcaatt 3720
ttgatgggtg ccaccttcgc ggaaatgaac actggaggag atgtagctca tctggcgctg 3780
atagcggcat tcaaagtcag accagcgttg ctggtatctt tcatcttcag agctaattgg 3840
acaccccgtg aaagcatgct gctggccttg gcctcgtgtc ttttgcaaac tgcgatctcc 3900
gccttggaag gcgacctgat ggttctcatc aatggttttg ctttggcctg gttggcaata 3960
cgagcgatgg ttgttccacg cactgataac atcaccttag caatcctggc tgctctgaca 4020
ccactggccc ggggcacact gcttgtggcg tggagagcag gccttgctac ttgcgggggg 4080
tttatgctcc tctctctgaa gggaaaaggc agtgtgaaga agaacttacc atttgtcatg 4140
gccctgggac taaccgctgt gaggctggtc gaccccatca acgtggtggg actgctgttg 4200
ctcacaagga gtgggaagcg gagctggccc cctagcgaag tactcacagc tgttggcctg 4260
atatgcgcat tggctggagg gttcgccaag gcagatatag agatggctgg gcccatggcc 4320
gcggtcggtc tgctaattgt cagttacgtg gtctcaggaa agagtgtgga catgtacatt 4380
gaaagagcag gtgacatcac atgggaaaaa gatgcggaag tcactggaaa cagtccccgg 4440
ctcgatgtgg cgctagatga gagtggtgat ttctccctgg tggaggatga cggtcccccc 4500
atgagagaga tcatactcaa ggtggtcctg atgaccatct gtggcatgaa cccaatagcc 4560
ataccctttg cagctggagc gtggtacgta tacgtgaaga ctggaaaaag gagtggtgct 4620
ctatgggatg tgcctgctcc caaggaagta aaaaaggggg agaccacaga tggagtgtac 4680
agagtaatga ctcgcagact gctaggttca acacaagttg gagtgggagt tatgcaagag 4740
ggggtctttc acactatgtg gcacgtcaca aaaggatccg cgctgagaag cggtgaaggg 4800
agacttgatc catactgggg agatgtcaag caggatctgg tgtcatactg tggtccatgg 4860
aagctagatg ccgcctggga cgggcacagc gaggtgcagc tcttggccgt gccccccgga 4920
gagagagcga ggaacatcca gactctgccc ggaatattta agacaaagga tggggacatt 4980
ggagcggttg cactggatta cccagcagga acttcaggat ctccaatcct agacaagtgt 5040
gggagagtga taggacttta tggcaatggg gtcgtgatca aaaatgggag ttatgttagt 5100
gccatcaccc aagggaggag ggaggaagag actcctgttg agtgcttcga gccttcgatg 5160
ctgaagaaga agcagctaac tgtcttagac ttgcatcctg gagctgggaa aaccaggaga 5220
gttcttcctg aaatagtccg tgaagccata aaaacaagac tccgtactgt gatcttggct 5280
ccaaccaggg ttgtcgctgc tgaaatggag gaggccctta gagggcttcc agtgcgttat 5340
atgacaacag cagtcaatgt cacccactct ggaacagaaa tcgtcgactt aatgtgccat 5400
gccaccttca cttcacgtct actacagcca attagagtcc ccaactataa tctgtatatt 5460
atggatgagg cccacttcac agatccctca agtatagcag caagaggata catttcaaca 5520
agggttgaga tgggcgaggc ggctgccatc ttcatgaccg ccacgccacc aggaacccgt 5580
gacgcatttc cggactccaa ctcaccaatt atggacaccg aagtggaagt cccagagaga 5640
gcctggagct caggctttga ttgggtgacg gagtattctg gaaaaacagt ttggtttgtt 5700
ccacgcgtga ggaacggcaa tgagatcgca gcttgtctga caaaggctgg aaaacgggtc 5760
atacagctca gcagaaagac ttttgagaca gagttccaga aaacaaaaca tcaagagtgg 5820
gactttgtcg tgacaactga catttcagag atgggcgcca actttaaagc tgaccgtgtc 5880
atagattcca ggagatgcct aaagccggtc atacttggtg gcgagagagt cattctggct 5940
ggacccatgc ctgtcacaca tgccagcgct gcccagagga gggggcgcat aggcaggaat 6000
cccaacaaac ctggagatga gtatctgtat ggaggtgggt gcgcagagac tgacgaagac 6060
catgcacact ggcttgaagc aagaatgctc cttgacaata tttacctcca agatggcctc 6120
atagcctcgc tctatcgacc tgaggccgac aaagtagcag ccattgaggg agagttcaag 6180
cttaggacgg agcaaaggaa gacctttgtg gaactcatga aaagaggaga tcttcctgtt 6240
tggctggcct atcaggttgc atctgccgga ataacctaca cagatagaag atggtgcttt 6300
gatggcacga ccaacaacac cataatggaa gacagtgtgc cggcagaggt gtggaccaga 6360
cacggagaga aaagagtgct caaaccgagg tggatggacg ccagagtttg ttcagatcat 6420
gcggccctga agtcattcaa ggagtttgcc gctgggaaaa gaggagcggc ttttggagtg 6480
atggaagccc tgggaacact gccaggacac atgacagaga gattccagga agccattgac 6540
aacctcgctg tgctcatgcg ggcagagact ggaagcaggc cttacaaagc cgcggcggcc 6600
caattgccgg agaccctaga gaccattatg cttttggggt tgctgggaac agtctcgctg 6660
ggaatctttt tcgtcttgat gaggaacaag ggcataggga agatgggctt tggaatggtg 6720
actcttgggg ccagcgcatg gctcatgtgg ctctcggaaa ttgagccagc cagaattgca 6780
tgtgtcctca ttgttgtgtt cctattgctg gtggtgctca tacctgagcc agaaaagcaa 6840
agatctcccc aggacaacca aatggccatc atcatcatgg tagcagtagg tcttctgggc 6900
ttgattaccg ccaatgaact cggatggttg gagagaacaa agagtgacct aagccatcta 6960
atgggaagga gagaggaggg ggcaaccatg ggattctcaa tggacattga cctgcggcca 7020
gcctcagctt gggccatcta tcctgccttg acatctttca ttaccccagc cgtccaacat 7080
gcagtgacca cttcatacaa caactactcc ttaatggcga tggccacgca agctggagtg 7140
ttgtttggta tgggcaaagg gatgccattc tacgcatggg actttggagt cccgctgcta 7200
atgataggtt gctactcaca attaacgccc ctgaccctaa tagtggccat cattttgctc 7260
gtggcgcact acatgtactt gatcccaggg ctgcaggcag cagctgcgcg tgctgcccag 7320
aagagaacgg cagctggcat catgaagaac cctgttgtgg agggaatagt ggtgactgac 7380
attgacacaa tgacaattga cccccaagtg gagaaaaaga tgggacaggt gctactcatg 7440
gcagtagccg tctccagcgc catactgtcg aggaccgcct gggggtgggg ggaggctggg 7500
gccctgatca cagccgcaac ttccactttg tgggaaggct ctccgaacaa gtactggaac 7560
tcctctacag ccacctcact gtgtaacatt tttaggggaa gttacttggc tggagcttct 7620
ctaatctaca cagtaacaag aaacgctggc ttggtcaaga gacgtggggg tggaacagga 7680
gagaccctgg gagagaaatg gaaggcccgc ttgaaccaga tgtcggccct ggagttctac 7740
tcctacaaaa agtcaggcat caccgaggtg tgcagagaag aggcccgccg cgccctcaag 7800
gacggtgtgg caacgggagg ccatgctgtg tcccgaggaa gtgcaaagct gagatggttg 7860
gtggagcggg gatacctgca gccctatgga aaggtcattg atcttggatg tggcagaggg 7920
ggctggagtt actacgccgc caccatccgc aaagttcaag aagtgaaagg atacacaaaa 7980
ggaggccctg gtcatgaaga acccgtgttg gtgcaaagct atgggtggaa catagtccgt 8040
cttaagagtg gggtggacgt ctttcatatg gcggctgagc cgtgtgacac gttgctgtgt 8100
gacataggtg agtcatcatc tagtcctgaa gtggaagaag cacggacgct cagagtcctc 8160
tccatggtgg gggattggct tgaaaaaaga ccaggagcct tttgtataaa agtgttgtgc 8220
ccatacacca gcactatgat ggaaaccctg gagcgactgc agcgtaggta tgggggagga 8280
ctggtcagag tgccactctc ccgcaactct acacatgaga tgtactgggt ctctggagcg 8340
aaaagcaaca ccataaaaag tgtgtccacc acgagccagc tcctcttggg gcgcatggac 8400
gggcctagga ggccagtgaa atatgaggag gatgtgaatc tcggctctgg cacgcgggct 8460
gtggtaagct gcgctgaagc tcccaacatg aagatcattg gtaaccgcat tgaaaggatc 8520
cgcgctgaga aagcggaaac gtggttcttt gacgagaacc acccatatag gacatgggct 8580
taccatggaa gctatgatgc cgccacacaa gggtcagcgt cctctctaat aaacggggtt 8640
gtcaggctcc tgtcaaaacc ctgggatgtg gtgactggag tcacaggaat agccatgacc 8700
gacaccacac cgtatggtca gcaaagagtt ttcaaggaaa aagtggacac tagggtgcca 8760
gacccccaag aaggcactcg tcaggttatg agcatggtct cttcctggtt gtggaaagag 8820
ctaggcaaac acaaacggcc acgagtctgt accaaagaag agttcatcaa caaggttcgt 8880
agcaatgcag cattaggggc aatatttgaa gaggaaaaag agtggaagac tgcagtggaa 8940
gctgtgaacg atccaaggtt ctgggctcta gtggacaagg aaagagagca ccacctgaga 9000
ggagagtgcc agagttgtgt gtacatcaca atgggaaaaa gagaaaagaa acaaggggaa 9060
tttggaaagg ccaagggcag ccgcgccatc tggtatatgt ggctaggggc tagatttcta 9120
gagttcgaag cccttggatt cttgaacgag gatcactgga tggggagaga gaactcagga 9180
ggtggtgttg aagggctggg attacaaaga ctcggatatg tcctagaaga gatgagtcgc 9240
ataccaggag gaaggatgta tgcagatgac actgctggct gggacacccg catcagcagg 9300
tttgatctgg agaatgaagc tctaatcacc aaccaaatgg agaaagggca cagggccttg 9360
gcattggcca taatcaagta cacataccaa aacaaagtgg taaaggtcct tagaccagct 9420
gaaaaaggga agacagttat ggacattatt tcgagacaag accaaagggg gagcggacaa 9480
gttgtcactt acgctctcaa cacatttacc aacctagtgg tgcaactcat tcggaatatg 9540
gaggctgagg aagttctaga gatgcaagac ttgtggctgc tgcggaggtc agagaaagtg 9600
accaactggt tgcagagcaa cggatgggat aggctcaaac gaatggcggt cagtggagat 9660
gattgcgttg tgaaaccaat tgatgatagg tttgcacatg ccctcaggtt cttgaatgat 9720
atgggaaaag ttaggaagga cacacaagag tggaaaccct caactggatg ggacaactgg 9780
gaagaagttc ccttctgctc ccaccacttc aacaagctcc atctcaagga cgggaggtcc 9840
attgtggttc cctgccgcca ccaagatgaa ctgattggcc gggcccgcgt ctctccaggg 9900
gcgggatgga gcatccggga gactgcttgc ctagcaaaat catatgcgca aatgtggcag 9960
ctcctttatt tccacagaag ggacctccga ctgatggcca atgccatttg ttcatctgtg 10020
ccagttgact gggttccaac tgggagaact acctggtcaa tccatggaaa gggagaatgg 10080
atgaccactg aagacatgct tgtggcgtgg aacagagtgt ggattgagga gaacgaccac 10140
atggaagaca agaccccagt cacgaaatgg acagacattc cctatttggg aaaaagggaa 10200
gacttgtggt gtggatctct catagggcac agaccgcgca ccacctgggc tgagaacatt 10260
aaaaacacag tcaacatggt gcgcaggatc ataggtgatg aagaaaagta catggactac 10320
ctatccaccc aagttcgcta cttgggtgaa gaagggtcta cacctggagt gctgtaagca 10380
ccaatcttaa tgttgtcagg cctgctagtc agccacagct tggggaaagc tgtgcagcct 10440
gtgacccccc caggagaagc tgggaaacca agcctatagt caggccgaga acgccatggc 10500
acggaagaag ccatgctgcc tgtgagcccc tcagaggaca ctgagtcaaa aaaccccacg 10560
cgcttggagg cgcaggatgg gaaaagaagg tggcgacctt ccccaccctt caatctgggg 10620
cctgaactgg agatcagctg tggatctcca gaagagggac tagtggttag aggaga 10676
<210> 11
<211> 10807
<212> DNA
<213> Zika病毒
<400> 11
agttgttgat ctgtgtgagt cagactgcga cagttcgagt ctgaagcgag agctaacaac 60
agtatcaaca ggtttaattt ggatttggaa acgagagttt ctggtcatga aaaacccaaa 120
gaagaaatcc ggaggattcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtaaa 180
ccccttggga ggtttgaaga ggttgccagc cggacttctg ctgggtcatg gacccatcag 240
aatggttttg gcgatactag cctttttgag atttacagca atcaagccat cactgggcct 300
tatcaacaga tggggttccg tggggaaaaa agaggctatg gaaataataa agaagttcaa 360
gaaagatctt gctgccatgt tgagaataat caatgctagg aaagagagga agagacgtgg 420
cgcagacacc agcatcggaa tcattggcct cctgctgact acagccatgg cagcagagat 480
cactagacgc gggagtgcat actacatgta cttggatagg agcgatgccg ggaaggccat 540
ttcgtttgct accacattgg gagtgaacaa gtgccacgta cagatcatgg acctcgggca 600
catgtgtgac gccaccatga gttatgagtg ccctatgctg gatgagggag tggaaccaga 660
tgatgtcgat tgctggtgca acacgacatc aacttgggtt gtgtacggaa cctgtcatca 720
caaaaaaggt gaggcacggc gatctagaag agccgtgacg ctcccttctc actctacaag 780
gaagttgcaa acgcggtcgc agacctggtt agaatcaaga gaatacacga agcacttgat 840
caaggttgaa aactggatat tcaggaaccc cgggtttgcg ctagtggccg ttgccattgc 900
ctggcttttg ggaagctcga cgagccaaaa agtcatatac ttggtcatga tactgctgat 960
tgccccggca tacagtatca ggtgcattgg agtcagcaat agagacttcg tggagggcat 1020
gtcaggtggg acctgggttg atgttgtctt ggaacatgga ggctgcgtta ccgtgatggc 1080
acaggacaag ccaacagttg acatagagtt ggtcacgacg acggttagta acatggccga 1140
ggtaagatcc tattgctacg aggcatcgat atcggacatg gcttcggaca gtcgttgccc 1200
aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac 1260
attagtggac agaggttggg gaaacggttg tggacttttt ggcaaaggga gcttggtgac 1320
atgtgccaag tttacgtgtt ctaagaagat gaccgggaag agcattcaac cggaaaatct 1380
ggagtatcgg ataatgctat cagtgcatgg ctcccagcat agcgggatga ctgtcaatga 1440
tataggatat gaaactgacg aaaatagagc gaaagtcgag gttacgccta attcaccaag 1500
agcggaagca accttgggag gctttggaag cttaggactt gactgtgaac caaggacagg 1560
ccttgacttt tcagatctgt attacctgac catgaacaat aagcattggt tggtgcacaa 1620
agagtggttt catgacatcc cattgccttg gcatgctggg gcagacactg gaactccaca 1680
ctggaacaac aaagaggcat tggtagaatt caaggatgcc cacgccaaga ggcaaaccgt 1740
cgtcgttctg gggagccagg aaggagccgt tcacacggct ctcgctggag ctctagaggc 1800
tgagatggat ggtgcaaagg gaaagctgtt ctctggccat ttgaaatgcc gcctaaaaat 1860
ggacaagctt agattgaagg gcgtgtcata ttccttgtgc actgcggcat tcacattcac 1920
caaggtccca gctgaaacac tgcatggaac agtcacagtg gaggtgcagt atgcagggac 1980
agatggaccc tgcaagatcc cagtccagat ggcggtggac atgcagaccc tgaccccagt 2040
tggaaggctg ataaccgcca accccgtgat tactgaaagc actgagaact caaagatgat 2100
gttggagctt gacccaccat ttggggattc ttacattgtc ataggagttg gggacaagaa 2160
aatcacccac cactggcata ggagtggtag caccatcgga aaggcatttg aggccactgt 2220
gagaggcgcc aagagaatgg cagtcctggg ggatacagcc tgggacttcg gatcagtcgg 2280
gggtgtgttc aactcactgg gtaagggcat tcaccagatt tttggagcag ccttcaaatc 2340
actgtttgga ggaatgtcct ggttctcaca gatcctcata ggcacgctgc tagtgtggtt 2400
aggtttgaac acaaagaatg gatctatctc cctcacatgc ttggccctgg ggggagtgat 2460
gatcttcctc tccacggctg tttctgctga cgtggggtgc tcagtggact tctcaaaaaa 2520
ggaaacgaga tgtggcacgg gggtattcat ctataatgat gttgaagcct ggagggaccg 2580
gtacaagtac catcctgact ccccccgcag attggcagca gcagtcaagc aggcctggga 2640
agaggggatc tgtgggatct catccgtttc aagaatggaa aacatcatgt ggaaatcagt 2700
agaaggggag ctcaatgcta tcctagagga gaatggagtt caactgacag ttgttgtggg 2760
atctgtaaaa aaccccatgt ggagaggtcc acaaagattg ccagtgcctg tgaatgagct 2820
gccccatggc tggaaagcct gggggaaatc gtattttgtt agggcggcaa agaccaacaa 2880
cagttttgtt gtcgacggtg acacactgaa ggaatgtccg cttgagcaca gagcatggaa 2940
tagttttctt gtggaggatc acgggtttgg agtcttccac accagtgtct ggcttaaggt 3000
cagagaagat tactcattag aatgtgaccc agccgtcata ggaacagctg ttaagggaag 3060
ggaggccgcg cacagtgatc tgggctattg gattgaaagt gaaaagaatg acacatggag 3120
gctgaagagg gcccacctga ttgagatgaa aacatgtgaa tggccaaagt ctcacacatt 3180
gtggacagat ggagtagaag aaagtgatct tatcataccc aagtctttag ctggtccact 3240
cagccaccac aacaccagag agggttacag aacccaagtg aaagggccat ggcacagtga 3300
agagcttgaa atccggtttg aggaatgtcc aggcaccaag gtttacgtgg aggagacatg 3360
cggaactaga ggaccatctc tgagatcaac tactgcaagt ggaagggtca ttgaggaatg 3420
gtgctgtagg gaatgcacaa tgcccccact atcgtttcga gcaaaagacg gctgctggta 3480
tggaatggag ataaggccca ggaaagaacc agagagcaac ttagtgaggt caatggtgac 3540
agcggggtca accgatcata tggaccactt ctctcttgga gtgcttgtga ttctactcat 3600
ggtgcaggag gggttgaaga agagaatgac cacaaagatc atcatgagca catcaatggc 3660
agtgctggta gtcatgatct tgggaggatt ttcaatgagt gacctggcca agcttgtgat 3720
cctgatgggt gctactttcg cagaaatgaa cactggagga gatgtagctc acttggcatt 3780
ggtagcggca tttaaagtca gaccagcctt gctggtctcc ttcattttca gagccaattg 3840
gacaccccgt gagagcatgc tgctagccct ggcttcgtgt cttctgcaaa ctgcgatctc 3900
tgctcttgaa ggtgacttga tggtcctcat taatggattt gctttggcct ggttggcaat 3960
tcgagcaatg gccgtgccac gcactgacaa catcgctcta ccaatcttgg ctgctctaac 4020
accactagct cgaggcacac tgctcgtggc atggagagcg ggcctggcta cttgtggagg 4080
gatcatgctc ctctccctga aagggaaagg tagtgtgaag aagaacctgc catttgtcat 4140
ggccctggga ttgacagctg tgagggtagt agaccctatt aatgtggtag gactactgtt 4200
actcacaagg agtgggaagc ggagctggcc ccctagtgaa gttctcacag ccgttggcct 4260
gatatgtgca ctggccggag ggtttgccaa ggcagacatt gagatggctg gacccatggc 4320
tgcagtaggc ttgctaattg tcagctatgt ggtctcggga aagagtgtgg acatgtacat 4380
tgaaagagca ggtgacatca catgggaaaa ggacgcggaa gtcactggaa acagtcctcg 4440
gcttgacgtg gcactggatg agagtggtga tttctccttg gtagaggaag atggtccacc 4500
catgagagag atcatactta aggtggtcct gatggccatc tgtggcatga acccaatagc 4560
tatacctttt gctgcaggag cgtggtatgt gtatgtgaag actgggaaaa ggagtggcgc 4620
cctctgggac gtgcctgctc ccaaagaagt gaagaaagga gagaccacag atggagtgta 4680
cagagtgatg actcgcagac tgctaggttc aacacaggtt ggagtgggag tcatgcaaga 4740
gggagtcttc cacaccatgt ggcacgttac aaaaggagcc gcactgagga gcggtgaggg 4800
aagacttgat ccatactggg gggatgtcaa gcaggacttg gtgtcatact gtgggccttg 4860
gaagttggat gcagcttggg atggactcag cgaggtacag cttttggccg tacctcccgg 4920
agagagggcc agaaacattc agaccctgcc tggaatattc aagacaaagg acggggacat 4980
cggagcagtt gctctggact accctgcagg gacctcagga tctccgatcc tagacaaatg 5040
tggaagagtg ataggactct atggcaatgg ggttgtgatc aagaatggaa gctatgttag 5100
tgctataacc cagggaaaga gggaggagga gactccggtt gaatgtttcg aaccctcgat 5160
gctgaagaag aagcagctaa ctgtcttgga tctgcatcca ggagccggaa aaaccaggag 5220
agttcttcct gaaatagtcc gtgaagccat aaaaaagaga ctccggacag tgatcttggc 5280
accaactagg gttgtcgctg ctgagatgga ggaggccttg agaggacttc cggtgcgtta 5340
catgacaaca gcagtcaacg tcacccattc tgggacagaa atcgttgatt tgatgtgcca 5400
tgccactttc acttcacgct tactacaacc catcagagtc cctaattaca atctctacat 5460
catggatgaa gcccacttca cagacccctc aagtatagct gcaagaggat atatatcaac 5520
aagggttgaa atgggcgagg cggctgccat ttttatgact gccacaccac caggaacccg 5580
tgatgcgttt cctgactcta actcaccaat catggacaca gaagtggaag tcccagagag 5640
agcctggagc tcaggctttg attgggtgac agaccattct gggaaaacag tttggttcgt 5700
tccaagcgtg agaaacggaa atgaaatcgc agcctgtctg acaaaggctg gaaagcgggt 5760
catacagctc agcaggaaga cttttgagac agaatttcag aaaacaaaaa atcaagagtg 5820
ggactttgtc ataacaactg acatctcaga gatgggcgcc aacttcaagg ctgaccgggt 5880
catagactct aggagatgcc taaaaccagt catacttgat ggtgagagag tcatcttggc 5940
tgggcccatg cctgtcacgc atgctagtgc tgctcagagg agaggacgta taggcaggaa 6000
ccctaacaaa cctggagatg agtacatgta tggaggtggg tgtgcagaga ctgatgaagg 6060
ccatgcacac tggcttgaag caagaatgct tcttgacaac atctacctcc aggatggcct 6120
catagcctcg ctctatcggc ctgaggccga taaggtagcc gccattgagg gagagtttaa 6180
gctgaggaca gagcaaagga agaccttcgt ggaactcatg aagagaggag accttcccgt 6240
ctggctagcc tatcaggttg catctgccgg aataacttac acagacagaa gatggtgctt 6300
tgatggcaca accaacaaca ccataatgga agacagcgta ccagcagagg tgtggacaaa 6360
gtatggagag aagagagtgc tcaaaccgag atggatggat gctagggtct gttcagacca 6420
tgcggccctg aagtcgttca aagaattcgc cgctggaaaa agaggagcgg ctttgggagt 6480
aatggaggcc ctgggaacac tgccaggaca catgacagag aggtttcagg aagccattga 6540
caacctcgcc gtgctcatgc gagcagagac tggaagcagg ccttataagg cagcggcagc 6600
ccaactgccg gagaccctag agaccattat gctcttaggt ttgctgggaa cagtttcact 6660
ggggatcttc ttcgtcttga tgcggaataa gggcatcggg aagatgggct ttggaatggt 6720
aacccttggg gccagtgcat ggctcatgtg gctttcggaa attgaaccag ccagaattgc 6780
atgtgtcctc attgttgtgt ttttattact ggtggtgctc atacccgagc cagagaagca 6840
aagatctccc caagataacc agatggcaat tatcatcatg gtggcagtgg gccttctagg 6900
tttgataact gcaaacgaac ttggatggct ggaaagaaca aaaaatgaca tagctcatct 6960
aatgggaagg agagaagaag gagcaaccat gggattctca atggacattg atctgcggcc 7020
agcctccgcc tgggctatct atgccgcatt gacaactctc atcaccccag ctgtccaaca 7080
tgcggtaacc acttcataca acaactactc cttaatggcg atggccacac aagctggagt 7140
gctgtttggc atgggcaaag ggatgccatt ttatgcatgg gaccttggag tcccgctgct 7200
aatgatgggt tgctattcac aattaacacc cctgactctg atagtagcta tcattctgct 7260
tgtggcgcac tacatgtact tgatcccagg cctacaagcg gcagcagcgc gtgctgccca 7320
gaaaaggaca gcagctggca tcatgaagaa tcccgttgtg gatggaatag tggtaactga 7380
cattgacaca atgacaatag acccccaggt ggagaagaag atgggacaag tgttactcat 7440
agcagtagcc atctccagtg ctgtgctgct gcggaccgcc tggggatggg gggaggctgg 7500
agctctgatc acagcagcga cctccacctt gtgggaaggc tctccaaaca aatactggaa 7560
ctcctctaca gccacctcac tgtgcaacat cttcagagga agctatctgg caggagcttc 7620
ccttatctat acagtgacga gaaacgctgg cctggttaag agacgtggag gtgggacggg 7680
agagactctg ggagagaagt ggaaagctcg tctgaatcag atgtcggccc tggagttcta 7740
ctcttataaa aagtcaggta tcactgaagt gtgtagagag gaggctcgcc gtgccctcaa 7800
ggatggagtg gccacaggag gacatgccgt atcccgggga agtgcaaagc tcagatggtt 7860
ggtggagaga ggatatctgc agccctatgg gaaggttgtt gacctcggat gtggcagagg 7920
gggctggagc tattatgccg ccaccatccg caaagtgcag gaggtgagag gatacacaaa 7980
gggaggtccc ggtcatgaag aacccatgct ggtgcaaagc tatgggtgga acatagttcg 8040
tctcaagagt ggagtggacg tcttccacat ggcggctgag ccgtgtgaca ctctgctgtg 8100
tgacataggt gagtcatcat ctagtcctga agtggaagag acacgaacac tcagagtgct 8160
ctctatggtg ggggactggc ttgaaaaaag accaggggcc ttctgtataa aggtgctgtg 8220
cccatacacc agcactatga tggaaaccat ggagcgactg caacgtaggc atgggggagg 8280
attagtcaga gtgccattgt ctcgcaactc cacacatgag atgtactggg tctctggggc 8340
aaagagcaac atcataaaaa gtgtgtccac cacaagtcag ctcctcctgg gacgcatgga 8400
tggccccagg aggccagtga aatatgagga ggatgtgaac ctcggctcgg gtacacgagc 8460
tgtggcaagc tgtgctgagg ctcctaacat gaaaatcatc ggcaggcgca ttgagagaat 8520
ccgcaatgaa catgcagaaa catggtttct tgatgaaaac cacccataca ggacatgggc 8580
ctaccatggg agctacgaag cccccacgca aggatcagcg tcttccctcg tgaacggggt 8640
tgttagactc ctgtcaaagc cttgggacgt ggtgactgga gttacaggaa tagccatgac 8700
tgacaccaca ccatacggcc aacaaagagt cttcaaagaa aaagtggaca ccagggtgcc 8760
agatccccaa gaaggcactc gccaggtaat gaacatagtc tcttcctggc tgtggaagga 8820
gctggggaaa cgcaagcggc cacgcgtctg caccaaagaa gagtttatca acaaggtgcg 8880
cagcaatgca gcactgggag caatatttga agaggaaaaa gaatggaaga cggctgtgga 8940
agctgtgaat gatccaaggt tttgggccct agtggatagg gagagagaac accacctgag 9000
aggagagtgt cacagctgtg tgtacaacat gatgggaaaa agagaaaaga agcaaggaga 9060
gttcgggaaa gcaaaaggta gccgcgccat ctggtacatg tggttgggag ccagattctt 9120
ggagtttgaa gcccttggat tcttgaacga ggaccattgg atgggaagag aaaactcagg 9180
aggtggagtc gaagggttag gattgcaaag acttggatac attctagaag aaatgaatcg 9240
ggcaccagga ggaaagatgt acgcagatga cactgctggc tgggacaccc gcattagtaa 9300
gtttgatctg gagaatgaag ctctgattac caaccaaatg gaggaagggc acagaactct 9360
ggcgttggcc gtgattaaat acacatacca aaacaaagtg gtgaaggttc tcagaccagc 9420
tgaaggagga aaaacagtta tggacatcat ttcaagacaa gaccagagag ggagtggaca 9480
agttgtcact tatgctctca acacattcac caacttggtg gtgcagctta tccggaacat 9540
ggaagctgag gaagtgttag agatgcaaga cttatggttg ttgaggaagc cagagaaagt 9600
gaccagatgg ttgcagagca atggatggga tagactcaaa cgaatggcgg tcagtggaga 9660
tgactgcgtt gtgaagccaa tcgatgatag gtttgcacat gccctcaggt tcttgaatga 9720
catgggaaaa gttaggaaag acacacagga gtggaaaccc tcgactggat ggagcaattg 9780
ggaagaagtc ccgttctgct cccaccactt caacaagctg tacctcaagg atgggagatc 9840
cattgtggtc ccttgccgcc accaagatga actgattggc cgagctcgcg tctcaccagg 9900
ggcaggatgg agcatccggg agactgcctg tcttgcaaaa tcatatgcgc agatgtggca 9960
gctcctttat ttccacagaa gagaccttcg actgatggct aatgccattt gctcggctgt 10020
gccagttgac tgggtaccaa ctgggagaac cacctggtca atccatggaa agggagaatg 10080
gatgaccact gaggacatgc tcatggtgtg gaatagagtg tggattgagg agaacgacca 10140
tatggaggac aagactcctg taacaaaatg gacagacatt ccctatctag gaaaaaggga 10200
ggacttatgg tgtggatccc ttatagggca cagaccccgc accacttggg ctgaaaacat 10260
caaagacaca gtcaacatgg tgcgcaggat cataggtgat gaagaaaagt acatggacta 10320
tctatccacc caagtccgct acttgggtga ggaagggtcc acacccggag tgttgtaagc 10380
accaatttta gtgttgtcag gcctgctagt cagccacagt ttggggaaag ctgtgcagcc 10440
tgtaaccccc ccaggagaag ctgggaaacc aagctcatag tcaggccgag aacgccatgg 10500
cacggaagaa gccatgctgc ctgtgagccc ctcagaggac actgagtcaa aaaaccccac 10560
gcgcttggaa gcgcaggatg ggaaaagaag gtggcgacct tccccaccct tcaatctggg 10620
gcctgaactg gagactagct gtgaatctcc agcagaggga ctagtggtta gaggagaccc 10680
cccggaaaac gcaaaacagc atattgacgc tgggaaagac cagagactcc atgagtttcc 10740
accacgctgg ccgccaggca cagatcgccg aacagcggcg gccggtgtgg ggaaatccat 10800
ggtttct 10807
<210> 12
<211> 10794
<212> DNA
<213> Zika病毒
<400> 12
agttgttgat ctgtgtgagt cagactgcga cagttcgagt ctgaagcgag agctaacaac 60
agtatcaaca ggtttaattt ggatttggaa acgagagttt ctggtcatga aaaaccccaa 120
agaagaaatc cggaggatcc ggattgtcaa tatgctaaaa cgcggagtag cccgtgtaaa 180
ccccttggga ggtttgaaga ggttgccagc cggacttctg ctgggtcatg gacccatcag 240
aatggttttg gcgatactag cctttttgag atttacagca atcaagccat cactgggcct 300
tatcaacaga tggggttccg tggggaaaaa agaggctatg gaaataataa agaagttcaa 360
gaaagatctt gctgccatgt tgagaataat caatgctagg aaagagagga agagacgtgg 420
cgcagacacc agcatcggaa tcattggcct cctgctgact acagccatgg cagcagagat 480
cactagacgc gggagtgcat actacatgta cttggatagg agcgatgccg ggaaggccat 540
ttcgtttgct accacattgg gagtgaacaa gtgccacgta cagatcatgg acctcgggca 600
catgtgtgac gccaccatga gttatgagtg ccctatgctg gatgagggag tggaaccaga 660
tgatgtcgat tgctggtgca acacgacatc aacttgggtt gtgtacggaa cctgtcatca 720
caaaaaaggt gaggcacggc gatctagaag agccgtgacg ctcccttctc actctacaag 780
gaagttgcaa acgcggtcgc agacctggtt agaatcaaga gaatacacga agcacttgat 840
caaggttgaa aactggatat tcaggaaccc cgggtttgcg ctagtggccg ttgccattgc 900
ctggcttttg ggaagctcga cgagccaaaa agtcatatac ttggtcatga tactgctgat 960
tgccccggca tacagtatca ggtgcattgg agtcagcaat agagacttcg tggagggcat 1020
gtcaggtggg acctgggttg atgttgtctt ggaacatgga ggctgcgtta ccgtgatggc 1080
acaggacaag ccaacagtcg acatagagtt ggtcacgacg acggttagta acatggccga 1140
ggtaagatcc tattgctacg aggcatcgat atcggacatg gcttcggaca gtcgttgccc 1200
aacacaaggt gaagcctacc ttgacaagca atcagacact caatatgtct gcaaaagaac 1260
attagtggac agaggttggg gaaacggttg tggacttttt ggcaaaggga gcttggtgac 1320
atgtgccaag tttacgtgtt ctaagaagat gaccgggaag agcattcaac cggaaaatct 1380
ggagtatcgg ataatgctat cagtgcatgg ctcccagcat agcgggatga ttggatatga 1440
aactgacgaa gatagagcga aagtcgaggt tacgcctaat tcaccaagag cggaagcaac 1500
cttgggaggc tttggaagct taggacttga ctgtgaacca aggacaggcc ttgacttttc 1560
agatctgtat tacctgacca tgaacaataa gcattggttg gtgcacaaag agtggtttca 1620
tgacatccca ttgccttggc atgctggggc agacaccgga actccacact ggaacaacaa 1680
agaggcattg gtagaattca aggatgccca cgccaagagg caaaccgtcg tcgttctggg 1740
gagccaggaa ggagccgttc acacggctct cgctggagct ctagaggctg agatggatgg 1800
tgcaaaggga aggctgttct ctggccattt gaaatgccgc ctaaaaatgg acaagcttag 1860
attgaagggc gtgtcatatt ccttgtgcac tgcggcattc acattcacca aggtcccagc 1920
tgaaacactg catggaacag tcacagtgga ggtgcagtat gcagggacag atggaccctg 1980
caagatccca gtccagatgg cggtggacat gcagaccctg accccagttg gaaggctgat 2040
aaccgccaac cccgtgatta ctgaaagcac tgagaactca aagatgatgt tggagcttga 2100
cccaccattt ggggattctt acattgtcat aggagttggg gacaagaaaa tcacccacca 2160
ctggcatagg agtggtagca ccatcggaaa ggcatttgag gccactgtga gaggcgccaa 2220
gagaatggca gtcctggggg atacagcctg ggacttcgga tcagtcgggg gtgtgttcaa 2280
ctcactgggt aagggcattc accagatttt tggagcagcc ttcaaatcac tgtttggagg 2340
aatgtcctgg ttctcacaga tcctcatagg cacgctgcta gtgtggttag gtttgaacac 2400
aaagaatgga tctatctccc tcacatgctt ggccctgggg ggagtgatga tcttcctctc 2460
cacggctgtt tctgctgacg tggggtgctc agtggacttc tcaaaaaagg aaacgagatg 2520
tggcacgggg gtattcatct ataatgatgt tgaagcctgg agggaccggt acaagtacca 2580
tcctgactcc ccccgcagat tggcagcagc agtcaagcag gcctgggaag aggggatctg 2640
tgggatctca tccgtttcaa gaatggaaaa catcatgtgg aaatcagtag aaggggagct 2700
caatgctatc ctagaggaga atggagttca actgacagtt gttgtgggat ctgtaaaaaa 2760
ccccatgtgg agaggtccac aaagattgcc agtgcctgtg aatgagctgc cccatggctg 2820
gaaagcctgg gggaaatcgt attttgttag ggcggcaaag accaacaaca gttttgttgt 2880
cgacggtgac acactgaagg aatgtccgct tgagcacaga gcatggaata gttttcttgt 2940
ggaggatcac gggtttggag tcttccacac cagtgtctgg cttaaggtca gagaagatta 3000
ctcattagaa tgtgacccag ccgtcatagg aacagctgtt aagggaaggg aggccgcgca 3060
cagtgatctg ggctattgga ttgaaagtga aaagaatgac acatggaggc tgaagagggc 3120
ccacctgatt gagatgaaaa catgtgaatg gccaaagtct cacacattgt ggacagatgg 3180
agtagaagaa agtgatctta tcatacccaa gtctttagct ggtccactca gccaccacaa 3240
caccagagag ggttacagaa cccaagtgaa agggccatgg cacagtgaag agcttgaaat 3300
ccggtttgag gaatgtccag gcaccaaggt ttacgtggag gagacatgcg gaactagagg 3360
accatctctg agatcaacta ctgcaagtgg aagggtcatt gaggaatggt gctgtaggga 3420
atgcacaatg cccccactat cgtttcgagc aaaagacggc tgctggtatg gaatggagat 3480
aaggcccagg aaagaaccag agagcaactt agtgaggtca atggtgacag cggggtcaac 3540
cgatcatatg gaccacttct ctcttggagt gcttgtgatt ctactcatgg tgcaggaggg 3600
gttgaagaag agaatgacca caaagatcat catgagcaca tcaatggcag tgctggtagt 3660
catgatcttg ggaggatttt caatgagtga cctggccaag cttgtgatcc tgatgggtgc 3720
tactttcgca gaaatgaaca ctggaggaga tgtagctcac ttggcattgg tagcggcatt 3780
taaagtcaga ccagccttgc tggtctcctt cattttcaga gccaattgga caccccgtga 3840
gagcatgctg ctagccctgg cttcgtgtct tctgcaaact gcgatctctg ctcttgaagg 3900
tgacttgatg gtcctcatta atggatttgc tttggcctgg ttggcaattc gagcaatggc 3960
cgtgccacgc actgacaaca tcgctctacc aatcttggct gctctaacac cactagctcg 4020
aggcacactg ctcgtggcat ggagagcggg cctggctact tgtggaggga tcatgctcct 4080
ctccctgaaa gggaaaggta gtgtgaagaa gaacctgcca tttgtcatgg ccctgggatt 4140
gacagctgtg agggtagtag accctattaa tgtggtagga ctactgttac tcacaaggag 4200
tgggaagcgg agctggcccc ctagtgaagt tctcacagcc gttggcctga tatgtgcact 4260
ggccggaggg tttgccaagg cagacattga gatggctgga cccatggctg cagtaggctt 4320
gctaattgtc agctatgtgg tctcgggaaa gagtgtggac atgtacattg aaagagcagg 4380
tgacatcaca tgggaaaagg acgcggaagt cactggaaac agtcctcggc ttgacgtggc 4440
actggatgag agtggtgact tctccttggt agaggaagat ggtccaccca tgagagagat 4500
catactcaag gtggtcctga tggccatctg tggcatgaac ccaatagcta taccttttgc 4560
tgcaggagcg tggtatgtgt atgtgaagac tgggaaaagg agtggcgccc tctgggacgt 4620
gcctgctccc aaagaagtga agaaaggaga gaccacagat ggagtgtaca gagtgatgac 4680
tcgcagactg ctaggttcaa cacaggttgg agtgggagtc atgcaagagg gagtcttcca 4740
caccatgtgg cacgttacaa aaggagccgc actgaggagc ggtgagggaa gacttgatcc 4800
atactggggg gatgtcaagc aggacttggt gtcatactgt gggccttgga agttggatgc 4860
agcttgggat ggactcagcg aggtacagct tttggccgta cctcccggag agagggccag 4920
aaacattcag accctgcctg gaatattcaa gacaaaggac ggggacatcg gagcagttgc 4980
tctggactac cctgcaggga cctcaggatc tccgatccta gacaaatgtg gaagagtgat 5040
aggactctat ggcaatgggg ttgtgatcaa gaatggaagc tatgttagtg ctataaccca 5100
gggaaagagg gaggaggaga ctccggttga atgtttcgaa ccctcgatgc tgaagaagaa 5160
gcagctaact gtcttggatc tgcatccagg agccggaaaa accaggagag ttcttcctga 5220
aatagtccgt gaagccataa aaaagagact ccggacagtg atcttggcac caactagggt 5280
tgtcgctgct gagatggagg aggccttgag aggacttccg gtgcgttaca tgacaacagc 5340
agtcaacgtc acccattctg ggacagaaat cgttgatttg atgtgccatg ccactttcac 5400
ttcacgctta ctacaaccca tcagagtccc taattacaat ctcaacatca tggatgaagc 5460
ccacttcaca gacccctcaa gtatagctgc aagaggatac atatcaacaa gggttgaaat 5520
gggcgaggcg gctgccattt ttatgactgc cacaccacca ggaacccgtg atgcgtttcc 5580
tgactctaac tcaccaatca tggacacaga agtggaagtc ccagagagag cctggagctc 5640
aggctttgat tgggtgacag accattctgg gaaaacagtt tggttcgttc caagcgtgag 5700
aaacggaaat gaaatcgcag cctgtctgac aaaggctgga aagcgggtca tacagctcag 5760
caggaagact tttgagacag aatttcagaa aacaaaaaat caagagtggg actttgtcat 5820
aacaactgac atctcagaga tgggcgccaa cttcaaggct gaccgggtca tagactctag 5880
gagatgccta aaaccagtca tacttgatgg tgagagagtc atcttggctg ggcccatgcc 5940
tgtcacgcat gctagtgctg ctcagaggag aggacgtata ggcaggaacc ctaacaaacc 6000
tggagatgag tacatgtatg gaggtgggtg tgcagagact gatgaaggcc atgcacactg 6060
gcttgaagca agaatgcttc ttgacaacat ctacctccag gatggcctca tagcctcgct 6120
ctatcggcct gaggccgata aggtagccgc cattgaggga gagtttaagc tgaggacaga 6180
gcaaaggaag accttcgtgg aactcatgaa gagaggagac cttcccgtct ggctagccta 6240
tcaggttgca tctgccggaa taacttacac agacagaaga tggtgctttg atggcacaac 6300
caacaacacc ataatggaag acagtgtacc agcagaggtt tggacaaagt atggagagaa 6360
gagagtgctc aaaccgagat ggatggatgc tagggtctgt tcagaccatg cggccctgaa 6420
gtcgttcaaa gaattcgccg ctggaaaaag aggagcggct ttgggagtaa tggaggccct 6480
gggaacactg ccaggacaca tgacagagag gtttcaggaa gccattgaca acctcgccgt 6540
gctcatgcga gcagagactg gaagcaggcc ttataaggca gcggcagccc aactgccgga 6600
gaccctagag accattatgc tcttaggttt gctgggaaca gtttcactgg ggatcttctt 6660
cgtcttgatg cggaataagg gcatcgggaa gatgggcttt ggaatggtaa cccttggggc 6720
cagtgcatgg ctcatgtggc tttcggaaat tgaaccagcc agaattgcat gtgtcctcat 6780
tgttgtgttt ttattactgg tggtgctcat acccgagcca gagaagcaaa gatctcccca 6840
agataaccag atggcaatta tcatcatggt ggcagtgggc cttctaggtt tgataactgc 6900
aaacgaactt ggatggctgg aaagaacaaa aaatgacata gctcatctaa tgggaaggag 6960
agaagaagga gcaaccatgg gattctcaat ggacattgat ctgcggccag cctccgcctg 7020
ggctatctat gccgcattga caactctcat caccccagct gtccaacatg cggtaaccac 7080
ttcatacaac aactactcct taatggcgat ggccacacaa gctggagtgc tgtttggcat 7140
gggcaaaggg atgccattta tgcatgggga ccttggagtc ccgctgctaa tgatgggttg 7200
ctattcacaa ttaacacccc tgactctgat agtagctatc attctgcttg tggcgcacta 7260
catgtacttg atcccaggcc tacaagcggc agcagcgcgt gctgcccaga aaaggacagc 7320
agctggcatc atgaagaatc ccgttgtgga tggaatagtg gtaactgaca ttgacacaat 7380
gacaatagac ccccaggtgg agaagaagat gggacaagtg ttactcatag cagtagccat 7440
ctccagtgct gtgctgctgc ggaccgcctg gggatggggg gaggctggag ctctgatcac 7500
agcagcgacc tccaccttgt gggaaggctc tccaaacaaa tactggaact cctctacagc 7560
cacctcactg tgcaacatct tcagaggaag ctatctggca ggagcttccc ttatctatac 7620
agtgacgaga aacgctggcc tggttaagag acgtggaggt gggacgggag agactctggg 7680
agagaagtgg aaagctcgtc tgaatcagat gtcggccctg gagttctact cttataaaaa 7740
gtcaggtatc actgaagtgt gtagagagga ggctcgccgt gccctcaagg atggagtggc 7800
cacaggagga catgccgtat cccggggaag tgcaaagatc agatggttgg aggagagagg 7860
atatctgcag ccctatggga aggttgttga cctcggatgt ggcagagggg gctggagcta 7920
ttatgccgcc accatccgca aagtgcagga ggtgagagga tacacaaagg gaggtcccgg 7980
tcatgaagaa cccatgctgg tgcaaagcta tgggtggaac atagttcgtc tcaagagtgg 8040
agtggacgtc ttccacatgg cggctgagcc gtgtgacact ctgctgtgtg acataggtga 8100
gtcatcatct agtcctgaag tggaagagac acgaacactc agagtgctct ctatggtggg 8160
ggactggctt gaaaaaagac caggggcctt ctgtataaag gtgctgtgcc catacaccag 8220
cactatgatg gaaaccatgg agcgactgca acgtaggcat gggggaggat tagtcagagt 8280
gccattgtgt cgcaactcca cacatgagat gtactgggtc tctggggcaa agagcaacat 8340
cataaaaagt gtgtccacca caagtcagct cctcctggga cgcatggatg gccccaggag 8400
gccagtgaaa tatgaggagg atgtgaacct cggctcgggt acacgagctg tggcaagctg 8460
tgctgaggct cctaacatga aaatcatcgg caggcgcatt gagagaatcc gcaatgaaca 8520
tgcagaaaca tggtttcttg atgaaaacca cccatacagg acatgggcct accatgggag 8580
ctacgaagcc cccacgcaag gatcagcgtc ttccctcgtg aacggggttg ttagactcct 8640
gtcaaagcct tgggacgtgg tgactggagt tacaggaata gccatgactg acaccacacc 8700
atacggccaa caaagagtct tcaaagaaaa agtggacacc agggtgccag atccccaaga 8760
aggcactcgc caggtaatga acatagtctc ttcctggctg tggaaggagc tggggaaacg 8820
caagcggcca cgcgtctgca ccaaagaaga gtttatcaac aaggtgcgca gcaatgcagc 8880
actgggagca atatttgaag aggaaaaaga atggaagacg gctgtggaag ctgtgaatga 8940
tccaaggttt tgggccctag tggataggga gagagaacac cacctgagag gagagtgtca 9000
cagctgtgtg tacaacatga tgggaaaaag agaaaagaag caaggagagt tcgggaaagc 9060
aaaaggtagc cgcgccatct ggtacatgtg gttgggagcc agattcttgg agtttgaagc 9120
ccttggattc ttgaacgagg accattggat gggaagagaa aactcaggag gtggagtcga 9180
agggttagga ttgcaaagac ttggatacat tctagaagaa atgaatcggg caccaggagg 9240
aaagatgtac gcagatgaca ctgctggctg ggacacccgc attagtaagt ttgatctgga 9300
gaatgaagct ctgattacca accaaatgga ggaagggcac agaactctgg cgttggccgt 9360
gattaaatac acataccaaa acaaagtggt gaaggttctc agaccagctg aaggaggaaa 9420
aacagttatg gacatcattt caagacaaga ccagagaggg agtggacaag ttgtcactta 9480
tgctctcaac acattcacca acttggtggt gcagcttatc cggaacatgg aagctgagga 9540
agtgttagag atgcaagact tatggttgtt gaggaagcca gagaaagtga ccagatggtt 9600
gcagagcaat ggatgggata gactcaaacg aatggcggtc agtggagatg actgcgttgt 9660
gaagccaatc gatgataggt ttgcacatgc cctcaggttc ttgaatgaca tgggaaaagt 9720
taggaaagac acacaggagt ggaaaccctc gactggatgg agcaattggg aagaagtccc 9780
gttctgctcc caccacttca acaagctgta cctcaaggat gggagatcca ttgtggtccc 9840
ttgccgccac caagatgaac tgattggccg agctcgcgtc tcaccagggg caggatggag 9900
catccgggag actgcctgtc ttgcaaaatc atatgcgcag atgtggcagc tcctttattt 9960
ccacagaaga gaccttcgac tgatggctaa tgccatttgc tcggctgtgc cagttgactg 10020
ggtaccaact gggagaacca cctggtcaat ccatggaaag ggagaatgga tgaccactga 10080
ggacatgctc atggtgtgga atagagtgtg gattgaggag aacgaccata tggaggacaa 10140
gactcctgta acaaaatgga cagacattcc ctatctagga aaaagggagg acttatggtg 10200
tggatccctt atagggcaca gaccccgcac cacttgggct gaaaacatca aagacacagt 10260
caacatggtg cgcaggatca taggtgatga agaaaagtac atggactatc tatccaccca 10320
agtccgctac ttgggtgagg aagggtccac acccggagtg ttgtaagcac caattttagt 10380
gttgtcaggc ctgctagtca gccacagttt ggggaaagct gtgcagcctg taaccccccc 10440
aggagaagct gggaaaccaa gctcatagtc aggccgagaa cgccatggca cggaagaagc 10500
catgctgcct gtgagcccct cagaggacac tgagtcaaaa aaccccacgc gcttggaagc 10560
gcaggatggg aaaagaaggt ggcgaccttc cccacccttc aatctggggc ctgaactgga 10620
gactagctgt gaatctccag cagagggact agtggttaga ggagaccccc cggaaaacgc 10680
aaaacagcat attgacgtgg gaaagaccag agactccatg agtttccacc acgctggccg 10740
ccaggcacag atcgccgaac ttcggcggcc ggtgtgggga aatccatggt ttct 10794
<210> 13
<211> 10617
<212> DNA
<213> Zika病毒
<400> 13
agtatcaaca ggttttattt tggatttgga aacgagagtt tctggtcatg aaaaacccaa 60
aaaagaaatc cggaggattc cggattgtca atatgctaaa acgcggagta gcccgtgtga 120
gcccctttgg gggcttgaag aggctgccag ccggacttct gctgggtcat gggcccatca 180
ggatggtctt ggcgattcta gcctttttga gattcacggc aatcaagcca tcactgggtc 240
tcatcaatag atggggttca gtggggaaaa aagaggctat ggaaataata aagaagttca 300
agaaagatct ggctgccatg ctgagaataa tcaatgctag gaaggagaag aagagacgag 360
gcgcagatac tagtgtcgga attgttggcc tcctgctgac cacagctatg gcagcggagg 420
tcactagacg tgggagtgca tactatatgt acttggacag aaacgacgct ggggaggcca 480
tatcttttcc aaccacattg gggatgaata agtgttatat acagatcatg gatcttggac 540
acatgtgtga tgccaccatg agctatgaat gccctatgct ggatgagggg gtggaaccag 600
atgacgtcga ttgttggtgc aacacgacgt caacttgggt tgtgtacgga acctgccatc 660
acaaaaaagg tgaagcacgg agatctagaa gagctgtgac gctcccctcc cattccacta 720
ggaagctgca aacgcggtcg caaacctggt tggaatcaag agaatacaca aagcacttga 780
ttagagtcga aaattggata ttcaggaacc ctggcttcgc gttagcagca gctgccatcg 840
cttggctttt gggaagctca acgagccaaa aagtcatata cttggtcatg atactgctga 900
ttgccccggc atacagcatc aggtgcatag gagtcagcaa tagggacttt gtggaaggta 960
tgtcaggtgg gacttgggtt gatgttgtct tggaacatgg aggttgtgtc accgtaatgg 1020
cacaggacaa accgactgtc gacatagagc tggttacaac aacagtcagc aacatggcgg 1080
aggtaagatc ctactgctat gaggcatcaa tatcggacat ggcttcggac agccgctgcc 1140
caacacaagg tgaagcctac cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa 1200
cgttagtgga cagaggctgg ggaaatggat gtggactttt tggcaaaggg agcctggtga 1260
catgcgctaa gtttgcatgc tccaagaaaa tgaccgggaa gagcatccag ccagagaatc 1320
tggagtaccg gataatgctg tcagttcatg gctcccagca cagtgggatg atcgttaatg 1380
acacaggaca tgaaactgat gagaatagag cgaaggttga gataacgccc aattcaccaa 1440
gagccgaagc caccctgggg ggttttggaa gcctaggact tgattgtgaa ccgaggacag 1500
gccttgactt ttcagatttg tattacttga ctatgaataa caagcactgg ttggttcaca 1560
aggagtggtt ccacgacatt ccattacctt ggcacgctgg ggcagacacc ggaactccac 1620
actggaacaa caaagaagca ctggtagagt tcaaggacgc acatgccaaa aggcaaactg 1680
tcgtggttct agggagtcaa gaaggagcag ttcacacggc ccttgctgga gctctggagg 1740
ctgagatgga tggtgcaaag ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa 1800
tggataaact tagattgaag ggcgtgtcat actccttgtg taccgcagcg ttcacattca 1860
ccaagatccc ggctgaaaca ctgcacggga cagtcacagt ggaggtacag tacgcaggga 1920
cagatggacc ttgcaaggtt ccagctcaga tggcggtgga catgcaaact ctgaccccag 1980
ttgggaggtt gataaccgct aaccccgtaa tcactgaaag cactgagaac tctaagatga 2040
tgctggaact tgatccacca tttggggact cttacattgt cataggagtc ggggagaaga 2100
agatcaccca ccactggcac aggagtggca gcaccattgg aaaagcattt gaagccactg 2160
tgagaggtgc caagagaatg gcagtcttgg gagacacagc ctgggacttt ggatcagttg 2220
gaggcgctct caactcattg ggcaagggca tccatcaaat ttttggagca gctttcaaat 2280
cattgtttgg aggaatgtcc tggttctcac aaattctcat tggaacgttg ctgatgtggt 2340
tgggtctgaa cacaaagaat ggatctattt cccttatgtg cttggcctta gggggagtgt 2400
tgatcttctt atccacagct gtctctgctg atgtggggtg ctcggtggac ttctcaaaga 2460
aggagacgag atgcggtaca ggggtgttcg tctataacga cgttgaagcc tggagggaca 2520
ggtacaagta ccatcctgac tccccccgta gattggcagc agcagtcaag caagcctggg 2580
aagatggtat ctgtgggatc tcctctgttt caagaatgga aaacatcatg tggagatcag 2640
tagaagggga gctcaacgca atcctggaag agaatggagt tcaactgacg gtcgttgtgg 2700
gatctgtaaa aaaccccatg tggagaggtc cacagagatt gcccgtgcct gtgaacgagc 2760
tgccccacgg ctggaaggct tgggggaaat cgtacttcgt cagagcagca aagacaaata 2820
acagctttgt cgtggatggt gacacactga aggaatgccc actcaaacat agagcatgga 2880
acagctttct tgtggaggat catgggttcg gggtatttca cactagtgtc tggctcaagg 2940
ttagagaaga ttattcatta gagtgtgatc cagccgttat tggaacagct gttaagggaa 3000
aggaggctgt acacagtgat ctaggctact ggattgagag tgagaagaat gacacatgga 3060
ggctgaagag ggcccatctg atcgagatga aaacatgtga atggccaaag tcccacacat 3120
tgtggacaga tggaatagaa gagagtgatc tgatcatacc caagtcttta gctgggccac 3180
tcagccatca caataccaga gagggctaca ggacccaaat gaaagggcca tggcacagtg 3240
aagagcttga aattcggttt gaggaatgcc caggcactaa ggtccacgtg gaggaaacat 3300
gtggaacaag aggaccatct ctgagatcaa ccactgcaag cggaagggtg atcgaggaat 3360
ggtgctgcag ggagtgcaca atgcccccac tgtcgttccg ggctaaagat ggctgttggt 3420
atggaatgga gataaggccc aggaaagaac cagaaagtaa cttagtaagg tcaatggtga 3480
ctgcaggatc aactgatcac atggatcact tctcccttgg agtgcttgtg attctgctca 3540
tggtgcagga agggctgaag aagagaatga ccacaaagat catcataagc acatcgatgg 3600
cagtgctggt agctatgatc ctgggaggat tttcaatgag tgacctggct aagcttgcaa 3660
ttttgatggg tgccaccttc gcggaaatga acactggagg agatgtagct catctggcgc 3720
tgatagcggc attcaaagtc agaccagcgt tgctggtatc tttcatcttc agagctaatt 3780
ggacaccccg tgaaagcatg ctgctggcct tggcctcgtg tcttttgcaa actgcgatct 3840
ccgccttgga aggcgacctg atggttctca tcaatggttt tgctttggcc tggttggcaa 3900
tacgagcgat ggttgttcca cgcactgata acatcacctt ggcaatcctg gctgctctga 3960
caccactggc ccggggcaca ctgcttgtgg cgtggagagc aggccttgct acttgcgggg 4020
ggtttatgct cctctctctg aagggaaaag gcagtgtgaa gaagaactta ccatttgtca 4080
tggccctggg actaaccgct gtgaggctgg tcgaccccat caacgtggtg ggactgctgt 4140
tgctcacaag gagtgggaag cggagctggc cccctagcga agtactcaca gctgttggcc 4200
tgatatgcgc attggctgga gggttcgcca aggcagatat agagatggct gggcccatgg 4260
ccgcggtcgg tctgctaatt gtcagttacg tggtctcagg aaagagtgtg gacatgtaca 4320
ttgaaagagc aggtgacatc acatgggaaa aagatgcgga agtcactgga aacagtcccc 4380
ggctcgatgt ggcgctagat gagagtggtg atttctccct ggtggaggat gacggtcccc 4440
ccatgagaga gatcatactc aaggtggtcc tgatgaccat ctgtggcatg aacccaatag 4500
ccataccctt tgcagctgga gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg 4560
ctctatggga tgtgcctgct cccaaggaag taaaaaaggg ggagaccaca gatggagtgt 4620
acagagtaat gactcgtaga ctgctaggtt caacacaagt tggagtggga gttatgcaag 4680
agggggtctt tcacactatg tggcacgtca caaaaggatc cgcgctgaga agcggtgaag 4740
ggagacttga tccatactgg ggagatgtca agcaggatct ggtgtcatac tgtggtccat 4800
ggaagctaga tgccgcctgg gacgggcaca gcgaggtgca gctcttggcc gtgccccccg 4860
gagagagagc gaggaacatc cagactctgc ccggaatatt taagacaaag gatggggaca 4920
ttggagcggt tgcgctggat tacccagcag gaacttcagg atctccaatc ctagacaagt 4980
gtgggagagt gataggactt tatggcaatg gggtcgtgat caaaaatggg agttatgtta 5040
gtgccatcac ccaagggagg agggaggaag agactcctgt tgagtgcttc gagccttcga 5100
tgctgaagaa gaagcagcta actgtcttag acttgcatcc tggagctggg aaaaccagga 5160
gagttcttcc tgaaatagtc cgtgaagcca taaaaacaag actccgtact gtgatcttag 5220
ctccaaccag ggttgtcgct gctgaaatgg aggaagccct tagagggctt ccagtgcgtt 5280
atatgacaac agcagtcaat gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc 5340
atgccacctt cacttcacgt ctactacagc caatcagagt ccccaactat aatctgtata 5400
ttatggatga ggcccacttc acagatccct caagtatagc agcaagagga tacatttcaa 5460
caagggttga gatgggcgag gcggctgcca tcttcatgac cgccacgcca ccaggaaccc 5520
gtgacgcatt tccggactcc aactcaccaa ttatggacac cgaagtggaa gtcccagaga 5580
gagcctggag ctcaggcttt gattgggtga cggatcattc tggaaaaaca gtttggtttg 5640
ttccaagcgt gaggaacggc aatgagatcg cagcttgtct gacaaaggct ggaaaacggg 5700
tcatacagct cagcagaaag acttttgaga cagagttcca gaaaacaaaa catcaagagt 5760
gggactttgt cgtgacaact gacatttcag agatgggcgc caactttaaa gctgaccgtg 5820
tcatagattc caggagatgc ctaaagccgg tcatacttga tggcgagaga gtcattctgg 5880
ctggacccat gcctgtcaca catgccagcg ctgcccagag gagggggcgc ataggcagga 5940
atcccaacaa acctggagat gagtatctgt atggaggtgg gtgcgcagag actgacgaag 6000
accatgcaca ctggcttgaa gcaagaatgc tccttgacaa tatttacctc caagatggcc 6060
tcatagcctc gctctatcga cctgaggccg acaaagtagc agccattgag ggagagttca 6120
agcttaggac ggagcaaagg aagacctttg tggaactcat gaaaagagga gatcttcctg 6180
tttggctggc ctatcaggtt gcatctgccg gaataaccta cacagataga agatggtgct 6240
ttgatggcac gaccaacaac accataatgg aagacagtgt gccggcagag gtgtggacca 6300
gacacggaga gaaaagagtg ctcaaaccga ggtggatgga cgccagagtt tgttcagatc 6360
atgcggccct gaagtcattc aaggagtttg ccgctgggaa aagaggagcg gcttttggag 6420
tgatggaagc cctgggaaca ctgccaggac acatgacaga gagattccag gaagccattg 6480
acaacctcgc tgtgctcatg cgggcagaga ctggaagcag gccttacaaa gccgcggcgg 6540
cccaattgcc ggagacccta gagaccatta tgcttttggg gttgctggga acagtctcgc 6600
tgggaatctt tttcgtcttg atgaggaaca agggcatagg gaagatgggc tttggaatgg 6660
tgactcttgg ggccagcgca tggctcatgt ggctctcgga aattgagcca gccagaattg 6720
catgtgtcct cattgttgtg ttcctattgc tggtggtgct catacctgag ccagaaaagc 6780
aaagatctcc ccaggacaac caaatggcaa tcatcatcat ggtagcagta ggtcttctgg 6840
gcttgattac cgccaatgaa ctcggatggt tggagagaac aaagagtgac ctaagccatc 6900
taatgggaag gagagaggag ggggcaacca taggattctc aatggacatt gacctgcggc 6960
cagcctcagc ttgggccatc tatgctgcct tgacaacttt cattacccca gccgtccaac 7020
atgcagtgac cacttcatac aacaactact ccttaatggc gatggccacg caagctggag 7080
tgttgtttgg tatgggcaaa gggatgccat tctacgcatg ggactttgga gtcccgctgc 7140
taatgatagg ttgctactca caattaacac ccctgaccct aatagtggcc atcattttgc 7200
tcgtggcgca ctacatgtac ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc 7260
agaagagaac ggcagctggc atcatgaaga accctgttgt ggatggaata gtggtgactg 7320
acattgacac aatgacaatt gacccccaag tggagaaaaa gatgggacag gtgctactca 7380
tagcagtagc cgtctccagc gccatactgt cgcggaccgc ctgggggtgg ggggaggctg 7440
gggccctgat cacagcggca acttccactt tgtgggaagg ctctccgaac aagtactgga 7500
actcctctac agccacttca ctgtgtaaca tttttagggg aagttacttg gctggagctt 7560
ctctaatcta cacagtaaca agaaacgctg gcttggtcaa gagacgtggg ggtggaacag 7620
gagagaccct gggagagaaa tggaaggccc gcttgaacca gatgtcggcc ctggagttct 7680
actcctacaa aaagtcaggc atcaccgagg tgtgcagaga agaggcccgc cgcgccctca 7740
aggacggtgt ggcaacggga ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt 7800
tggtggagcg gggatacctg cagccctatg gaaaggtcat tgatcttgga tgtggcagag 7860
ggggctggag ttactacgcc gccaccatcc gcaaagttca agaagtgaaa ggatacacaa 7920
aaggaggccc tggtcatgaa gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc 7980
gtcttaagag tggggtggac gtctttcata tggcggctga gccgtgtgac acgttgctgt 8040
gtgacatagg tgagtcatca tctagtcctg aagtggaaga agcacggacg ctcagagtcc 8100
tctccatggt gggggattgg cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt 8160
gcccatacac cagcactatg atggaaaccc tggagcgact gcagcgtagg tatgggggag 8220
gactggtcag agtgccactc tcccgcaact ctacacatga gatgtactgg gtctctggag 8280
cgaaaagcaa caccataaaa agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg 8340
acgggcccag gaggccagtg aaatatgagg aggatgtgaa tctcggctct ggcacgcggg 8400
ctgtggtaag ctgcgctgaa gctcccaaca tgaagatcat tggtaaccgc attgaaagga 8460
tccgcagtga gcacgcggaa acgtggttct ttgacgagaa ccacccatat aggacatggg 8520
cttaccatgg aagctatgag gcccccacac aagggtcagc gtcctctcta ataaacgggg 8580
ttgtcaggct cctgtcaaaa ccctgggatg tggtgactgg agtcacagga atagccatga 8640
ccgacaccac accgtatggt cagcaaagag ttttcaagga aaaagtggac actagggtgc 8700
cagaccccca agaaggcact cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag 8760
agctaggcaa acacaaacgg ccacgagtct gtaccaaaga agagttcatc aacaaggttc 8820
gtagcaatgc agcattaggg gcaatatttg aagaggaaaa agagtggaag actgcagtgg 8880
aagctgtgaa cgatccaagg ttctgggctc tagtggacaa ggaaagagag caccacctga 8940
gaggagagtg ccagagttgt gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg 9000
aatttggaaa ggccaagggc agccgcgcca tctggtatat gtggctaggg gctagatttc 9060
tagagttcga agcccttgga ttcttgaacg aggatcactg gatggggaga gagaactcag 9120
gaggtggtgt tgaagggctg ggattacaaa gactcggata tgtcctagaa gagatgagtc 9180
gcataccagg aggaaggatg tatgcagatg acactgctgg ctgggacacc cgcatcagca 9240
ggtttgatct ggagaatgaa gctctaatca ccaaccaaat ggagaaaggg cacagggcct 9300
tggcattggc cataatcaag tacacatacc aaaacaaagt ggtaaaggtc cttagaccag 9360
ctgaaaaagg gaagacagtt atggacatta tttcgagaca agaccaaagg gggagcggac 9420
aagttgtcac ttacgctctt aacacattta ccaacctagt ggtgcaactc attcggaata 9480
tggaggctga ggaagttcta gagatgcaag acttgtggct gctgcggagg tcagagaaag 9540
tgaccaactg gttgcagagc aacggatggg ataggctcaa acgaatggca gtcagtggag 9600
atgattgcgt tgtgaagcca attgatgata ggtttgcaca tgccctcagg ttcttgaatg 9660
atatgggaaa agttaggaag gacacacaag agtggaaacc ctcaactgga tgggacaact 9720
gggaagaagt tccgttttgc tcccaccact tcaacaagct ccatctcaag gacgggaggt 9780
ccattgtggt tccctgccgc caccaagatg aactgattgg ccgggcccgc gtctctccag 9840
gggcgggatg gagcatccgg gagactgctt gcctagcaaa atcatatgcg caaatgtggc 9900
agctccttta tttccacaga agggacctcc gactgatggc caatgccatt tgttcatctg 9960
tgccagttga ctgggttcca actgggagaa ctacctggtc aatccatgga aagggagaat 10020
ggatgaccac tgaagacatg cttgtggtgt ggaacagagt gtggattgag gagaacgacc 10080
acatggaaga caagacccca gttacgaaat ggacagacat tccctatttg ggaaaaaggg 10140
aagacttgtg gtgtggatct ctcatagggc acagaccgcg caccacctgg gctgagaaca 10200
ttaaaaacac agtcaacatg gtgcgcagga tcataggtga tgaagaaaag tacatggact 10260
acctatccac ccaagttcgc tacttgggtg aagaagggtc tacacctgga gtgctgtaag 10320
caccaatctt agtgttgtca ggcctgctag tcagccacag cttggggaaa gctgtgcagc 10380
ctgtgacccc cccaggagaa gctgggaaac caagcctata gtcaggccga gaacgccatg 10440
gcacggaaga agccatgctg cctgtgagcc cctcagagga cactgagtca aaaaacccca 10500
cgcgcttgga ggcgcaggat gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg 10560
ggcctgaact ggagatcagc tgtggatctc cagaagaggg actagtggtt agaggag 10617
<210> 14
<211> 498
<212> PRT
<213> Zika病毒
<400> 14
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg Ala Lys
145 150 155 160
Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly
165 170 175
Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe
180 185 190
Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His
195 200 205
Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp
210 215 220
Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys
225 230 235 240
Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu
245 250 255
Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp
260 265 270
Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys
275 280 285
Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala
290 295 300
Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val
305 310 315 320
Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro
325 330 335
Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu
340 345 350
Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met
355 360 365
Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly
370 375 380
Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr
385 390 395 400
Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala
405 410 415
Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe
420 425 430
Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys
435 440 445
Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr
450 455 460
Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu
465 470 475 480
Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val
485 490 495
Ser Ala
<210> 15
<211> 498
<212> PRT
<213> Zika病毒
<400> 15
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg Ala Lys
145 150 155 160
Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly
165 170 175
Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe
180 185 190
Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His
195 200 205
Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ser Gly Ala Asp
210 215 220
Thr Glu Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys
225 230 235 240
Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu
245 250 255
Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp
260 265 270
Gly Ala Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys
275 280 285
Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala
290 295 300
Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val
305 310 315 320
Thr Val Glu Val Gln Tyr Ala Gly Arg Asp Gly Pro Cys Lys Val Pro
325 330 335
Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu
340 345 350
Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met
355 360 365
Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly
370 375 380
Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Ile
385 390 395 400
Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala
405 410 415
Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe
420 425 430
Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys
435 440 445
Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr
450 455 460
Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu
465 470 475 480
Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val
485 490 495
Ser Ala
<210> 16
<211> 504
<212> PRT
<213> Zika病毒
<220>
<221> misc_feature
<222> (156)..(162)
<223> X=any amino acid
<400> 16
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Xaa Xaa Xaa Xaa Xaa
145 150 155 160
Xaa Xaa Asn Arg Ala Glu Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 17
<211> 504
<212> PRT
<213> Zika病毒
<220>
<221> misc_feature
<222> (152)..(156)
<223> X=any amino acid
<400> 17
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Xaa Xaa Xaa Xaa Xaa Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Arg Leu Val Arg Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
Leu Lys Lys Gly Ser Ser Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Val His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 18
<211> 504
<212> PRT
<213> Zika病毒
<400> 18
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 19
<211> 504
<212> PRT
<213> Zika病毒
<400> 19
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 20
<211> 504
<212> PRT
<213> Zika病毒
<400> 20
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Val His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 21
<211> 504
<212> PRT
<213> Zika病毒
<400> 21
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Val His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 22
<211> 504
<212> PRT
<213> Zika病毒
<400> 22
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 23
<211> 504
<212> PRT
<213> Zika病毒
<400> 23
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 24
<211> 504
<212> PRT
<213> Zika病毒
<400> 24
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Thr Val Asn Asp Ile Gly Tyr Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 25
<211> 504
<212> PRT
<213> Zika病毒
<400> 25
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly Tyr Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Lys Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 26
<211> 504
<212> PRT
<213> Zika病毒
<400> 26
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Val Cys Thr Ala Ala Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 27
<211> 500
<212> PRT
<213> Zika病毒
<400> 27
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg
145 150 155 160
Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu
165 170 175
Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu
180 185 190
Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu
195 200 205
Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly
210 215 220
Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu
225 230 235 240
Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser
245 250 255
Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu
260 265 270
Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg
275 280 285
Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys
290 295 300
Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly
305 310 315 320
Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys
325 330 335
Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly
340 345 350
Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser
355 360 365
Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val
370 375 380
Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly
385 390 395 400
Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg
405 410 415
Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly
420 425 430
Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala
435 440 445
Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile
450 455 460
Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile
465 470 475 480
Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr
485 490 495
Ala Val Ser Ala
500
<210> 28
<211> 500
<212> PRT
<213> Zika病毒
<400> 28
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg
145 150 155 160
Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu
165 170 175
Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu
180 185 190
Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu
195 200 205
Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly
210 215 220
Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu
225 230 235 240
Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser
245 250 255
Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu
260 265 270
Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg
275 280 285
Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys
290 295 300
Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly
305 310 315 320
Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys
325 330 335
Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly
340 345 350
Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser
355 360 365
Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val
370 375 380
Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly
385 390 395 400
Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg
405 410 415
Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly
420 425 430
Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala
435 440 445
Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile
450 455 460
Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile
465 470 475 480
Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr
485 490 495
Ala Val Ser Ala
500
<210> 29
<211> 500
<212> PRT
<213> Zika病毒
<400> 29
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Gly Tyr Glu Thr Asp Glu Asp Arg
145 150 155 160
Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu
165 170 175
Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu
180 185 190
Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu
195 200 205
Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly
210 215 220
Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu
225 230 235 240
Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser
245 250 255
Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu
260 265 270
Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg
275 280 285
Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys
290 295 300
Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly
305 310 315 320
Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys
325 330 335
Ile Pro Val Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly
340 345 350
Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser
355 360 365
Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val
370 375 380
Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly
385 390 395 400
Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg
405 410 415
Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly
420 425 430
Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala
435 440 445
Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile
450 455 460
Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile
465 470 475 480
Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr
485 490 495
Ala Val Ser Ala
500
<210> 30
<211> 504
<212> PRT
<213> Zika病毒
<400> 30
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Thr Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Gly
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 31
<211> 504
<212> PRT
<213> Zika病毒
<400> 31
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Thr Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Gly
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 32
<211> 504
<212> PRT
<213> Zika病毒
<400> 32
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 33
<211> 504
<212> PRT
<213> Zika病毒
<400> 33
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 34
<211> 504
<212> PRT
<213> Zika病毒
<400> 34
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 35
<211> 504
<212> PRT
<213> Zika病毒
<400> 35
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 36
<211> 504
<212> PRT
<213> Zika病毒
<400> 36
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 37
<211> 504
<212> PRT
<213> Zika病毒
<400> 37
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 38
<211> 504
<212> PRT
<213> Zika病毒
<400> 38
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 39
<211> 504
<212> PRT
<213> Zika病毒
<400> 39
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 40
<211> 504
<212> PRT
<213> Zika病毒
<400> 40
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 41
<211> 504
<212> PRT
<213> Zika病毒
<400> 41
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 42
<211> 504
<212> PRT
<213> Zika病毒
<400> 42
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 43
<211> 504
<212> PRT
<213> Zika病毒
<400> 43
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 44
<211> 504
<212> PRT
<213> Zika病毒
<400> 44
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 45
<211> 504
<212> PRT
<213> Zika病毒
<400> 45
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 46
<211> 504
<212> PRT
<213> Zika病毒
<400> 46
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 47
<211> 504
<212> PRT
<213> Zika病毒
<400> 47
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 48
<211> 504
<212> PRT
<213> Zika病毒
<400> 48
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 49
<211> 504
<212> PRT
<213> Zika病毒
<400> 49
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 50
<211> 504
<212> PRT
<213> Zika病毒
<400> 50
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Ile Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 51
<211> 504
<212> PRT
<213> Zika病毒
<400> 51
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Ile Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 52
<211> 504
<212> PRT
<213> Zika病毒
<400> 52
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Thr Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 53
<211> 504
<212> PRT
<213> Zika病毒
<400> 53
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Thr Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 54
<211> 504
<212> PRT
<213> Zika病毒
<400> 54
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Arg Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 55
<211> 504
<212> PRT
<213> Zika病毒
<400> 55
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 56
<211> 504
<212> PRT
<213> Zika病毒
<400> 56
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 57
<211> 504
<212> PRT
<213> Zika病毒
<400> 57
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Ala Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 58
<211> 504
<212> PRT
<213> Zika病毒
<400> 58
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Thr Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 59
<211> 504
<212> PRT
<213> Zika病毒
<400> 59
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Gly Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 60
<211> 504
<212> PRT
<213> Zika病毒
<400> 60
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Leu Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 61
<211> 504
<212> PRT
<213> Zika病毒
<400> 61
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Ala Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 62
<211> 504
<212> PRT
<213> Zika病毒
<400> 62
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 63
<211> 504
<212> PRT
<213> Zika病毒
<400> 63
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 64
<211> 504
<212> PRT
<213> Zika病毒
<400> 64
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Ser Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Thr
50 55 60
Ile Ser Asp Ile Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Ala Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 65
<211> 504
<212> PRT
<213> Zika病毒
<220>
<221> misc_feature
<222> (1)..(504)
<223> X=any amino acid
<400> 65
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Xaa Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
Xaa Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 66
<211> 504
<212> PRT
<213> Zika病毒
<400> 66
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Ala Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Leu Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Ala His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Ala Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Val Asp Gly Thr Val Thr Val Glu Gly Gln Tyr Gly Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Ile Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Gly
500
<210> 67
<211> 504
<212> PRT
<213> Zika病毒
<400> 67
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Ala Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Leu Val Asn Asp Thr Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Ala His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Ala Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu
305 310 315 320
Thr Val Asp Gly Thr Val Thr Val Glu Gly Gln Tyr Gly Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Ile Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Gly
500
<210> 68
<211> 504
<212> PRT
<213> Zika病毒
<400> 68
Ile Ser Cys Ile Gly Val Ser Asn Arg Asp Leu Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Glu Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Met
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Leu Ser Asp Met Ala Ser Ala Ser Arg Cys Pro Thr Gln Gly Glu Pro
65 70 75 80
Ser Leu Asp Lys Gln Ser Asp Thr Gln Ser Val Cys Lys Arg Thr Leu
85 90 95
Gly Asp Arg Gly Trp Gly Asn Gly Cys Gly Ile Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ser Lys Phe Thr Cys Cys Lys Lys Met Pro Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Pro Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Ile Gly His Glu Thr
145 150 155 160
Asp Glu Asn Arg Ala Lys Val Glu Val Thr Pro Asn Ser Pro Arg Ala
165 170 175
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro
180 185 190
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn
195 200 205
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro
210 215 220
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu
225 230 235 240
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val
245 250 255
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala
260 265 270
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Phe Ser Gly His
275 280 285
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser
290 295 300
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Val Pro Ala Glu
305 310 315 320
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Ser Ala Gly Thr Asp
325 330 335
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu
340 345 350
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser
355 360 365
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp
370 375 380
Ser Tyr Ile Val Ile Gly Val Gly Asp Lys Lys Ile Thr His His Trp
385 390 395 400
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg
405 410 415
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly
420 425 430
Ser Val Gly Gly Val Phe Asn Ser Leu Gly Lys Gly Ile His Gln Ile
435 440 445
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser
450 455 460
Gln Ile Leu Ile Gly Thr Leu Leu Val Trp Leu Gly Leu Asn Thr Lys
465 470 475 480
Asn Gly Ser Ile Ser Leu Thr Cys Leu Ala Leu Gly Gly Val Met Ile
485 490 495
Phe Leu Ser Thr Ala Val Ser Ala
500
<210> 69
<211> 498
<212> PRT
<213> Zika病毒
<400> 69
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser
1 5 10 15
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr
20 25 30
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr
35 40 45
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser
50 55 60
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala
65 70 75 80
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu
85 90 95
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser
100 105 110
Leu Val Thr Cys Ala Lys Phe Thr Cys Ser Lys Lys Met Thr Gly Lys
115 120 125
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His
130 135 140
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Glu Asn Arg Ala Lys
145 150 155 160
Val Glu Val Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly
165 170 175
Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe
180 185 190
Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His
195 200 205
Lys Glu Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp
210 215 220
Thr Gly Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys
225 230 235 240
Asp Ala His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu
245 250 255
Gly Ala Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp
260 265 270
Gly Ala Lys Gly Arg Leu Phe Ser Gly His Leu Lys Cys Arg Leu Lys
275 280 285
Met Asp Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala
290 295 300
Ala Phe Thr Phe Thr Lys Val Pro Ala Glu Thr Leu His Gly Thr Val
305 310 315 320
Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro
325 330 335
Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu
340 345 350
Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met
355 360 365
Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly
370 375 380
Val Gly Asp Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr
385 390 395 400
Ile Gly Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala
405 410 415
Val Leu Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Val Phe
420 425 430
Asn Ser Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys
435 440 445
Ser Leu Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr
450 455 460
Leu Leu Val Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu
465 470 475 480
Thr Cys Leu Ala Leu Gly Gly Val Met Ile Phe Leu Ser Thr Ala Val
485 490 495
Ser Ala
<210> 70
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 1-26 n=inosine
<220>
<221> misc_feature
<222> (1)..(26)
<223> n=inosine
<400> 70
ncncncncnc ncncncncnc ncncnc 26
<210> 71
<211> 11
<212> PRT
<213> 人工序列
<220>
<223> 合成肽
<400> 71
Lys Leu Lys Leu Leu Leu Leu Leu Lys Leu Lys
1 5 10
<210> 72
<211> 10773
<212> DNA
<213> Zika病毒
<400> 72
cagactgcga cagttcgagt ttgaagcgaa agctagcaac agtatcaaca ggttttattt 60
tggatttgga aacgagagtt tctggtcatg aaaaacccaa aaaagaaatc cggaggattc 120
cggattgtca atatgctaaa acgcggagta gcccgtgtga gcccctttgg gggcttgaag 180
aggctgccag ccggacttct gctgggtcat gggcccatca ggatggtctt ggcgattcta 240
gcctttttga gattcacggc aatcaagcca tcactgggtc tcatcaatag atggggttca 300
gtggggaaaa aagaggctat ggaaataata aagaagttca agaaagatct ggctgccatg 360
ctgagaataa tcaatgctag gaaggagaag aagagacgag gcgcagatac tagtgtcgga 420
attgttggcc tcctgctgac cacagctatg gcagcggagg tcactagacg tgggagtgca 480
tactatatgt acttggacag aaacgacgct ggggaggcca tatcttttcc aaccacattg 540
gggatgaata agtgttatat acagatcatg gatcttggac acatgtgtga tgccaccatg 600
agctatgaat gccctatgct ggatgagggg gtggaaccag atgacgtcga ttgttggtgc 660
aacacgacgt caacttgggt tgtgtacgga acctgccatc acaaaaaagg tgaagcacgg 720
agatctagaa gagctgtgac gctcccctcc cattccacta ggaagctgca aacgcggtcg 780
caaacctggt tggaatcaag agaatacaca aagcacttga ttagagtcga aaattggata 840
ttcaggaacc ctggcttcgc gttagcagca gctgccatcg cttggctttt gggaagctca 900
acgagccaaa aagtcatata cttggtcatg atactgctga ttgccccggc atacagcatc 960
aggtgcatag gagtcagcaa tagggacttt gtggaaggta tgtcaggtgg gacttgggtt 1020
gatgttgtct tggaacatgg aggttgtgtc accgtaatgg cacaggacaa accgactgtc 1080
gacatagagc tggttacaac aacagtcagc aacatggcgg aggtaagatc ctactgctat 1140
gaggcatcaa tatcggacat ggcttcggac agccgctgcc caacacaagg tgaagcctac 1200
cttgacaagc aatcagacac tcaatatgtc tgcaaaagaa cgttagtgga cagaggctgg 1260
ggaaatggat gtggactttt tggcaaaggg agcctggtga catgcgctaa gtttgcatgc 1320
tccaagaaaa tgaccgggaa gagcatccag ccagagaatc tggagtaccg gataatgctg 1380
tcagttcatg gctcccagca cagtgggatg atcgttaatg acacaggaca tgaaactgat 1440
gagaatagag cgaaggttga gataacgccc aattcaccaa gagccgaagc caccctgggg 1500
ggttttggaa gcctaggact tgattgtgaa ccgaggacag gccttgactt ttcagatttg 1560
tattacttga ctatgaataa caagcactgg ttggttcaca aggagtggtt ccacgacatt 1620
ccattacctt ggcacgctgg ggcagacacc ggaactccac actggaacaa caaagaagca 1680
ctggtagagt tcaaggacgc acatgccaaa aggcaaactg tcgtggttct agggagtcaa 1740
gaaggagcag ttcacacggc ccttgctgga gctctggagg ctgagatgga tggtgcaaag 1800
ggaaggctgt cctctggcca cttgaaatgt cgcctgaaaa tggataaact tagattgaag 1860
ggcgtgtcat actccttgtg taccgcagcg ttcacattca ccaagatccc ggctgaaaca 1920
ctgcacggga cagtcacagt ggaggtacag tacgcaggga cagatggacc ttgcaaggtt 1980
ccagctcaga tggcggtgga catgcaaact ctgaccccag ttgggaggtt gataaccgct 2040
aaccccgtaa tcactgaaag cactgagaac tctaagatga tgctggaact tgatccacca 2100
tttggggact cttacattgt cataggagtc ggggagaaga agatcaccca ccactggcac 2160
aggagtggca gcaccattgg aaaagcattt gaagccactg tgagaggtgc caagagaatg 2220
gcagtcttgg gagacacagc ctgggacttt ggatcagttg gaggcgctct caactcattg 2280
ggcaagggca tccatcaaat ttttggagca gctttcaaat cattgtttgg aggaatgtcc 2340
tggttctcac aaattctcat tggaacgttg ctgatgtggt tgggtctgaa cacaaagaat 2400
ggatctattt cccttatgtg cttggcctta gggggagtgt tgatcttctt atccacagct 2460
gtctctgctg atgtggggtg ctcggtggac ttctcaaaga aggagacgag atgcggtaca 2520
ggggtgttcg tctataacga cgttgaagcc tggagggaca ggtacaagta ccatcctgac 2580
tccccccgta gattggcagc agcagtcaag caagcctggg aagatggtat ctgtgggatc 2640
tcctctgttt caagaatgga aaacatcatg tggagatcag tagaagggga gctcaacgca 2700
atcctggaag agaatggagt tcaactgacg gtcgttgtgg gatctgtaaa aaaccccatg 2760
tggagaggtc cacagagatt gcccgtgcct gtgaacgagc tgccccacgg ctggaaggct 2820
tgggggaaat cgtacttcgt cagagcagca aagacaaata acagctttgt cgtggatggt 2880
gacacactga aggaatgccc actcaaacat agagcatgga acagctttct tgtggaggat 2940
catgggttcg gggtatttca cactagtgtc tggctcaagg ttagagaaga ttattcatta 3000
gagtgtgatc cagccgttat tggaacagct gttaagggaa aggaggctgt acacagtgat 3060
ctaggctact ggattgagag tgagaagaat gacacatgga ggctgaagag ggcccatctg 3120
atcgagatga aaacatgtga atggccaaag tcccacacat tgtggacaga tggaatagaa 3180
gagagtgatc tgatcatacc caagtcttta gctgggccac tcagccatca caataccaga 3240
gagggctaca ggacccaaat gaaagggcca tggcacagtg aagagcttga aattcggttt 3300
gaggaatgcc caggcactaa ggtccacgtg gaggaaacat gtggaacaag aggaccatct 3360
ctgagatcaa ccactgcaag cggaagggtg atcgaggaat ggtgctgcag ggagtgcaca 3420
atgcccccac tgtcgttccg ggctaaagat ggctgttggt atggaatgga gataaggccc 3480
aggaaagaac cagaaagtaa cttagtaagg tcaatggtga ctgcaggatc aactgatcac 3540
atggatcact tctcccttgg agtgcttgtg attctgctca tggtgcagga agggctgaag 3600
aagagaatga ccacaaagat catcataagc acatcgatgg cagtgctggt agctatgatc 3660
ctgggaggat tttcaatgag tgacctggct aagcttgcaa ttttgatggg tgccaccttc 3720
gcggaaatga acactggagg agatgtagct catctggcgc tgatagcggc attcaaagtc 3780
agaccagcgt tgctggtatc tttcatcttc agagctaatt ggacaccccg tgaaagcatg 3840
ctgctggcct tggcctcgtg tcttttgcaa actgcgatct ccgccttgga aggcgacctg 3900
atggttctca tcaatggttt tgctttggcc tggttggcaa tacgagcgat ggttgttcca 3960
cgcactgata acatcacctt ggcaatcctg gctgctctga caccactggc ccggggcaca 4020
ctgcttgtgg cgtggagagc aggccttgct acttgcgggg ggtttatgct cctctctctg 4080
aagggaaaag gcagtgtgaa gaagaactta ccatttgtca tggccctggg actaaccgct 4140
gtgaggctgg tcgaccccat caacgtggtg ggactgctgt tgctcacaag gagtgggaag 4200
cggagctggc cccctagcga agtactcaca gctgttggcc tgatatgcgc attggctgga 4260
gggttcgcca aggcagatat agagatggct gggcccatgg ccgcggtcgg tctgctaatt 4320
gtcagttacg tggtctcagg aaagagtgtg gacatgtaca ttgaaagagc aggtgacatc 4380
acatgggaaa aagatgcgga agtcactgga aacagtcccc ggctcgatgt ggcgctagat 4440
gagagtggtg atttctccct ggtggaggat gacggtcccc ccatgagaga gatcatactc 4500
aaggtggtcc tgatgaccat ctgtggcatg aacccaatag ccataccctt tgcagctgga 4560
gcgtggtacg tatacgtgaa gactggaaaa aggagtggtg ctctatggga tgtgcctgct 4620
cccaaggaag taaaaaaggg ggagaccaca gatggagtgt acagagtaat gactcgtaga 4680
ctgctaggtt caacacaagt tggagtggga gttatgcaag agggggtctt tcacactatg 4740
tggcacgtca caaaaggatc cgcgctgaga agcggtgaag ggagacttga tccatactgg 4800
ggagatgtca agcaggatct ggtgtcatac tgtggtccat ggaagctaga tgccgcctgg 4860
gacgggcaca gcgaggtgca gctcttggcc gtgccccccg gagagagagc gaggaacatc 4920
cagactctgc ccggaatatt taagacaaag gatggggaca ttggagcggt tgcgctggat 4980
tacccagcag gaacttcagg atctccaatc ctagacaagt gtgggagagt gataggactt 5040
tatggcaatg gggtcgtgat caaaaatggg agttatgtta gtgccatcac ccaagggagg 5100
agggaggaag agactcctgt tgagtgcttc gagccttcga tgctgaagaa gaagcagcta 5160
actgtcttag acttgcatcc tggagctggg aaaaccagga gagttcttcc tgaaatagtc 5220
cgtgaagcca taaaaacaag actccgtact gtgatcttag ctccaaccag ggttgtcgct 5280
gctgaaatgg aggaagccct tagagggctt ccagtgcgtt atatgacaac agcagtcaat 5340
gtcacccact ctggaacaga aatcgtcgac ttaatgtgcc atgccacctt cacttcacgt 5400
ctactacagc caatcagagt ccccaactat aatctgtata ttatggatga ggcccacttc 5460
acagatccct caagtatagc agcaagagga tacatttcaa caagggttga gatgggcgag 5520
gcggctgcca tcttcatgac cgccacgcca ccaggaaccc gtgacgcatt tccggactcc 5580
aactcaccaa ttatggacac cgaagtggaa gtcccagaga gagcctggag ctcaggcttt 5640
gattgggtga cggatcattc tggaaaaaca gtttggtttg ttccaagcgt gaggaacggc 5700
aatgagatcg cagcttgtct gacaaaggct ggaaaacggg tcatacagct cagcagaaag 5760
acttttgaga cagagttcca gaaaacaaaa catcaagagt gggactttgt cgtgacaact 5820
gacatttcag agatgggcgc caactttaaa gctgaccgtg tcatagattc caggagatgc 5880
ctaaagccgg tcatacttga tggcgagaga gtcattctgg ctggacccat gcctgtcaca 5940
catgccagcg ctgcccagag gagggggcgc ataggcagga atcccaacaa acctggagat 6000
gagtatctgt atggaggtgg gtgcgcagag actgacgaag accatgcaca ctggcttgaa 6060
gcaagaatgc tccttgacaa tatttacctc caagatggcc tcatagcctc gctctatcga 6120
cctgaggccg acaaagtagc agccattgag ggagagttca agcttaggac ggagcaaagg 6180
aagacctttg tggaactcat gaaaagagga gatcttcctg tttggctggc ctatcaggtt 6240
gcatctgccg gaataaccta cacagataga agatggtgct ttgatggcac gaccaacaac 6300
accataatgg aagacagtgt gccggcagag gtgtggacca gacacggaga gaaaagagtg 6360
ctcaaaccga ggtggatgga cgccagagtt tgttcagatc atgcggccct gaagtcattc 6420
aaggagtttg ccgctgggaa aagaggagcg gcttttggag tgatggaagc cctgggaaca 6480
ctgccaggac acatgacaga gagattccag gaagccattg acaacctcgc tgtgctcatg 6540
cgggcagaga ctggaagcag gccttacaaa gccgcggcgg cccaattgcc ggagacccta 6600
gagaccatta tgcttttggg gttgctggga acagtctcgc tgggaatctt tttcgtcttg 6660
atgaggaaca agggcatagg gaagatgggc tttggaatgg tgactcttgg ggccagcgca 6720
tggctcatgt ggctctcgga aattgagcca gccagaattg catgtgtcct cattgttgtg 6780
ttcctattgc tggtggtgct catacctgag ccagaaaagc aaagatctcc ccaggacaac 6840
caaatggcaa tcatcatcat ggtagcagta ggtcttctgg gcttgattac cgccaatgaa 6900
ctcggatggt tggagagaac aaagagtgac ctaagccatc taatgggaag gagagaggag 6960
ggggcaacca taggattctc aatggacatt gacctgcggc cagcctcagc ttgggccatc 7020
tatgctgcct tgacaacttt cattacccca gccgtccaac atgcagtgac cacttcatac 7080
aacaactact ccttaatggc gatggccacg caagctggag tgttgtttgg tatgggcaaa 7140
gggatgccat tctacgcatg ggactttgga gtcccgctgc taatgatagg ttgctactca 7200
caattaacac ccctgaccct aatagtggcc atcattttgc tcgtggcgca ctacatgtac 7260
ttgatcccag ggctgcaggc agcagctgcg cgtgctgccc agaagagaac ggcagctggc 7320
atcatgaaga accctgttgt ggatggaata gtggtgactg acattgacac aatgacaatt 7380
gacccccaag tggagaaaaa gatgggacag gtgctactca tagcagtagc cgtctccagc 7440
gccatactgt cgcggaccgc ctgggggtgg ggggaggctg gggccctgat cacagcggca 7500
acttccactt tgtgggaagg ctctccgaac aagtactgga actcctctac agccacttca 7560
ctgtgtaaca tttttagggg aagttacttg gctggagctt ctctaatcta cacagtaaca 7620
agaaacgctg gcttggtcaa gagacgtggg ggtggaacag gagagaccct gggagagaaa 7680
tggaaggccc gcttgaacca gatgtcggcc ctggagttct actcctacaa aaagtcaggc 7740
atcaccgagg tgtgcagaga agaggcccgc cgcgccctca aggacggtgt ggcaacggga 7800
ggccatgctg tgtcccgagg aagtgcaaag ctgagatggt tggtggagcg gggatacctg 7860
cagccctatg gaaaggtcat tgatcttgga tgtggcagag ggggctggag ttactacgcc 7920
gccaccatcc gcaaagttca agaagtgaaa ggatacacaa aaggaggccc tggtcatgaa 7980
gaacccatgt tggtgcaaag ctatgggtgg aacatagtcc gtcttaagag tggggtggac 8040
gtctttcata tggcggctga gccgtgtgac acgttgctgt gtgacatagg tgagtcatca 8100
tctagtcctg aagtggaaga agcacggacg ctcagagtcc tctccatggt gggggattgg 8160
cttgaaaaaa gaccaggagc cttttgtata aaagtgttgt gcccatacac cagcactatg 8220
atggaaaccc tggagcgact gcagcgtagg tatgggggag gactggtcag agtgccactc 8280
tcccgcaact ctacacatga gatgtactgg gtctctggag cgaaaagcaa caccataaaa 8340
agtgtgtcca ccacgagcca gctcctcttg gggcgcatgg acgggcccag gaggccagtg 8400
aaatatgagg aggatgtgaa tctcggctct ggcacgcggg ctgtggtaag ctgcgctgaa 8460
gctcccaaca tgaagatcat tggtaaccgc attgaaagga tccgcagtga gcacgcggaa 8520
acgtggttct ttgacgagaa ccacccatat aggacatggg cttaccatgg aagctatgag 8580
gcccccacac aagggtcagc gtcctctcta ataaacgggg ttgtcaggct cctgtcaaaa 8640
ccctgggatg tggtgactgg agtcacagga atagccatga ccgacaccac accgtatggt 8700
cagcaaagag ttttcaagga aaaagtggac actagggtgc cagaccccca agaaggcact 8760
cgtcaggtta tgagcatggt ctcttcctgg ttgtggaaag agctaggcaa acacaaacgg 8820
ccacgagtct gtaccaaaga agagttcatc aacaaggttc gtagcaatgc agcattaggg 8880
gcaatatttg aagaggaaaa agagtggaag actgcagtgg aagctgtgaa cgatccaagg 8940
ttctgggctc tagtggacaa ggaaagagag caccacctga gaggagagtg ccagagttgt 9000
gtgtacaaca tgatgggaaa aagagaaaag aaacaagggg aatttggaaa ggccaagggc 9060
agccgcgcca tctggtatat gtggctaggg gctagatttc tagagttcga agcccttgga 9120
ttcttgaacg aggatcactg gatggggaga gagaactcag gaggtggtgt tgaagggctg 9180
ggattacaaa gactcggata tgtcctagaa gagatgagtc gcataccagg aggaaggatg 9240
tatgcagatg acactgctgg ctgggacacc cgcatcagca ggtttgatct ggagaatgaa 9300
gctctaatca ccaaccaaat ggagaaaggg cacagggcct tggcattggc cataatcaag 9360
tacacatacc aaaacaaagt ggtaaaggtc cttagaccag ctgaaaaagg gaagacagtt 9420
atggacatta tttcgagaca agaccaaagg gggagcggac aagttgtcac ttacgctctt 9480
aacacattta ccaacctagt ggtgcaactc attcggaata tggaggctga ggaagttcta 9540
gagatgcaag acttgtggct gctgcggagg tcagagaaag tgaccaactg gttgcagagc 9600
aacggatggg ataggctcaa acgaatggca gtcagtggag atgattgcgt tgtgaagcca 9660
attgatgata ggtttgcaca tgccctcagg ttcttgaatg atatgggaaa agttaggaag 9720
gacacacaag agtggaaacc ctcaactgga tgggacaact gggaagaagt tccgttttgc 9780
tcccaccact tcaacaagct ccatctcaag gacgggaggt ccattgtggt tccctgccgc 9840
caccaagatg aactgattgg ccgggcccgc gtctctccag gggcgggatg gagcatccgg 9900
gagactgctt gcctagcaaa atcatatgcg caaatgtggc agctccttta tttccacaga 9960
agggacctcc gactgatggc caatgccatt tgttcatctg tgccagttga ctgggttcca 10020
actgggagaa ctacctggtc aatccatgga aagggagaat ggatgaccac tgaagacatg 10080
cttgtggtgt ggaacagagt gtggattgag gagaacgacc acatggaaga caagacccca 10140
gttacgaaat ggacagacat tccctatttg ggaaaaaggg aagacttgtg gtgtggatct 10200
ctcatagggc acagaccgcg caccacctgg gctgagaaca ttaaaaacac agtcaacatg 10260
gtgcgcagga tcataggtga tgaagaaaag tacatggact acctatccac ccaagttcgc 10320
tacttgggtg aagaagggtc tacacctgga gtgctgtaag caccaatctt agtgttgtca 10380
ggcctgctag tcagccacag cttggggaaa gctgtgcagc ctgtgacccc cccaggagaa 10440
gctgggaaac caagcctata gtcaggccga gaacgccatg gcacggaaga agccatgctg 10500
cctgtgagcc cctcagagga cactgagtca aaaaacccca cgcgcttgga ggcgcaggat 10560
gggaaaagaa ggtggcgacc ttccccaccc ttcaatctgg ggcctgaact ggagatcagc 10620
tgtggatctc cagaagaggg actagtggtt agaggagacc ccccggaaaa cgcaaaacag 10680
catattgacg ctgggaaaga ccagagactc catgagtttc caccacgctg gccgccaggc 10740
acagatcgcc gaatagcggc ggccggtgtg ggg 10773
<210> 73
<211> 3423
<212> PRT
<213> Zika病毒
<400> 73
Met Lys Asn Pro Lys Lys Lys Ser Gly Gly Phe Arg Ile Val Asn Met
1 5 10 15
Leu Lys Arg Gly Val Ala Arg Val Ser Pro Phe Gly Gly Leu Lys Arg
20 25 30
Leu Pro Ala Gly Leu Leu Leu Gly His Gly Pro Ile Arg Met Val Leu
35 40 45
Ala Ile Leu Ala Phe Leu Arg Phe Thr Ala Ile Lys Pro Ser Leu Gly
50 55 60
Leu Ile Asn Arg Trp Gly Ser Val Gly Lys Lys Glu Ala Met Glu Ile
65 70 75 80
Ile Lys Lys Phe Lys Lys Asp Leu Ala Ala Met Leu Arg Ile Ile Asn
85 90 95
Ala Arg Lys Glu Lys Lys Arg Arg Gly Ala Asp Thr Ser Val Gly Ile
100 105 110
Val Gly Leu Leu Leu Thr Thr Ala Met Ala Ala Glu Val Thr Arg Arg
115 120 125
Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg Asn Asp Ala Gly Glu Ala
130 135 140
Ile Ser Phe Pro Thr Thr Leu Gly Met Asn Lys Cys Tyr Ile Gln Ile
145 150 155 160
Met Asp Leu Gly His Met Cys Asp Ala Thr Met Ser Tyr Glu Cys Pro
165 170 175
Met Leu Asp Glu Gly Val Glu Pro Asp Asp Val Asp Cys Trp Cys Asn
180 185 190
Thr Thr Ser Thr Trp Val Val Tyr Gly Thr Cys His His Lys Lys Gly
195 200 205
Glu Ala Arg Arg Ser Arg Arg Ala Val Thr Leu Pro Ser His Ser Thr
210 215 220
Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp Leu Glu Ser Arg Glu Tyr
225 230 235 240
Thr Lys His Leu Ile Arg Val Glu Asn Trp Ile Phe Arg Asn Pro Gly
245 250 255
Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp Leu Leu Gly Ser Ser Thr
260 265 270
Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala
275 280 285
Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly
290 295 300
Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys
305 310 315 320
Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val
325 330 335
Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu
340 345 350
Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly
355 360 365
Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg
370 375 380
Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys
385 390 395 400
Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr
405 410 415
Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser
420 425 430
Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His
435 440 445
Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro
450 455 460
Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys
465 470 475 480
Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met
485 490 495
Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro
500 505 510
Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn
515 520 525
Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr
530 535 540
Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala
545 550 555 560
Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser
565 570 575
Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly
580 585 590
Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro
595 600 605
Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly
610 615 620
Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln
625 630 635 640
Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr
645 650 655
Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe
660 665 670
Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His
675 680 685
His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr
690 695 700
Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp
705 710 715 720
Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His
725 730 735
Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp
740 745 750
Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn
755 760 765
Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val
770 775 780
Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val Gly Cys Ser Val
785 790 795 800
Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr
805 810 815
Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser
820 825 830
Pro Arg Arg Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile
835 840 845
Cys Gly Ile Ser Ser Val Ser Arg Met Glu Asn Ile Met Trp Arg Ser
850 855 860
Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly Val Gln Leu
865 870 875 880
Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg Gly Pro Gln
885 890 895
Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp Lys Ala Trp
900 905 910
Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn Ser Phe Val
915 920 925
Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His Arg Ala Trp
930 935 940
Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe His Thr Ser
945 950 955 960
Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala
965 970 975
Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His Ser Asp Leu
980 985 990
Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg
995 1000 1005
Ala His Leu Ile Glu Met Lys Thr Cys Glu Trp Pro Lys Ser His
1010 1015 1020
Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp Leu Ile Ile Pro
1025 1030 1035
Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr Arg Glu Gly
1040 1045 1050
Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu Leu Glu
1055 1060 1065
Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu Glu
1070 1075 1080
Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser
1085 1090 1095
Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro
1100 1105 1110
Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu
1115 1120 1125
Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met
1130 1135 1140
Val Thr Ala Gly Ser Thr Asp His Met Asp His Phe Ser Leu Gly
1145 1150 1155
Val Leu Val Ile Leu Leu Met Val Gln Glu Gly Leu Lys Lys Arg
1160 1165 1170
Met Thr Thr Lys Ile Ile Ile Ser Thr Ser Met Ala Val Leu Val
1175 1180 1185
Ala Met Ile Leu Gly Gly Phe Ser Met Ser Asp Leu Ala Lys Leu
1190 1195 1200
Ala Ile Leu Met Gly Ala Thr Phe Ala Glu Met Asn Thr Gly Gly
1205 1210 1215
Asp Val Ala His Leu Ala Leu Ile Ala Ala Phe Lys Val Arg Pro
1220 1225 1230
Ala Leu Leu Val Ser Phe Ile Phe Arg Ala Asn Trp Thr Pro Arg
1235 1240 1245
Glu Ser Met Leu Leu Ala Leu Ala Ser Cys Leu Leu Gln Thr Ala
1250 1255 1260
Ile Ser Ala Leu Glu Gly Asp Leu Met Val Leu Ile Asn Gly Phe
1265 1270 1275
Ala Leu Ala Trp Leu Ala Ile Arg Ala Met Val Val Pro Arg Thr
1280 1285 1290
Asp Asn Ile Thr Leu Ala Ile Leu Ala Ala Leu Thr Pro Leu Ala
1295 1300 1305
Arg Gly Thr Leu Leu Val Ala Trp Arg Ala Gly Leu Ala Thr Cys
1310 1315 1320
Gly Gly Phe Met Leu Leu Ser Leu Lys Gly Lys Gly Ser Val Lys
1325 1330 1335
Lys Asn Leu Pro Phe Val Met Ala Leu Gly Leu Thr Ala Val Arg
1340 1345 1350
Leu Val Asp Pro Ile Asn Val Val Gly Leu Leu Leu Leu Thr Arg
1355 1360 1365
Ser Gly Lys Arg Ser Trp Pro Pro Ser Glu Val Leu Thr Ala Val
1370 1375 1380
Gly Leu Ile Cys Ala Leu Ala Gly Gly Phe Ala Lys Ala Asp Ile
1385 1390 1395
Glu Met Ala Gly Pro Met Ala Ala Val Gly Leu Leu Ile Val Ser
1400 1405 1410
Tyr Val Val Ser Gly Lys Ser Val Asp Met Tyr Ile Glu Arg Ala
1415 1420 1425
Gly Asp Ile Thr Trp Glu Lys Asp Ala Glu Val Thr Gly Asn Ser
1430 1435 1440
Pro Arg Leu Asp Val Ala Leu Asp Glu Ser Gly Asp Phe Ser Leu
1445 1450 1455
Val Glu Asp Asp Gly Pro Pro Met Arg Glu Ile Ile Leu Lys Val
1460 1465 1470
Val Leu Met Thr Ile Cys Gly Met Asn Pro Ile Ala Ile Pro Phe
1475 1480 1485
Ala Ala Gly Ala Trp Tyr Val Tyr Val Lys Thr Gly Lys Arg Ser
1490 1495 1500
Gly Ala Leu Trp Asp Val Pro Ala Pro Lys Glu Val Lys Lys Gly
1505 1510 1515
Glu Thr Thr Asp Gly Val Tyr Arg Val Met Thr Arg Arg Leu Leu
1520 1525 1530
Gly Ser Thr Gln Val Gly Val Gly Val Met Gln Glu Gly Val Phe
1535 1540 1545
His Thr Met Trp His Val Thr Lys Gly Ser Ala Leu Arg Ser Gly
1550 1555 1560
Glu Gly Arg Leu Asp Pro Tyr Trp Gly Asp Val Lys Gln Asp Leu
1565 1570 1575
Val Ser Tyr Cys Gly Pro Trp Lys Leu Asp Ala Ala Trp Asp Gly
1580 1585 1590
His Ser Glu Val Gln Leu Leu Ala Val Pro Pro Gly Glu Arg Ala
1595 1600 1605
Arg Asn Ile Gln Thr Leu Pro Gly Ile Phe Lys Thr Lys Asp Gly
1610 1615 1620
Asp Ile Gly Ala Val Ala Leu Asp Tyr Pro Ala Gly Thr Ser Gly
1625 1630 1635
Ser Pro Ile Leu Asp Lys Cys Gly Arg Val Ile Gly Leu Tyr Gly
1640 1645 1650
Asn Gly Val Val Ile Lys Asn Gly Ser Tyr Val Ser Ala Ile Thr
1655 1660 1665
Gln Gly Arg Arg Glu Glu Glu Thr Pro Val Glu Cys Phe Glu Pro
1670 1675 1680
Ser Met Leu Lys Lys Lys Gln Leu Thr Val Leu Asp Leu His Pro
1685 1690 1695
Gly Ala Gly Lys Thr Arg Arg Val Leu Pro Glu Ile Val Arg Glu
1700 1705 1710
Ala Ile Lys Thr Arg Leu Arg Thr Val Ile Leu Ala Pro Thr Arg
1715 1720 1725
Val Val Ala Ala Glu Met Glu Glu Ala Leu Arg Gly Leu Pro Val
1730 1735 1740
Arg Tyr Met Thr Thr Ala Val Asn Val Thr His Ser Gly Thr Glu
1745 1750 1755
Ile Val Asp Leu Met Cys His Ala Thr Phe Thr Ser Arg Leu Leu
1760 1765 1770
Gln Pro Ile Arg Val Pro Asn Tyr Asn Leu Tyr Ile Met Asp Glu
1775 1780 1785
Ala His Phe Thr Asp Pro Ser Ser Ile Ala Ala Arg Gly Tyr Ile
1790 1795 1800
Ser Thr Arg Val Glu Met Gly Glu Ala Ala Ala Ile Phe Met Thr
1805 1810 1815
Ala Thr Pro Pro Gly Thr Arg Asp Ala Phe Pro Asp Ser Asn Ser
1820 1825 1830
Pro Ile Met Asp Thr Glu Val Glu Val Pro Glu Arg Ala Trp Ser
1835 1840 1845
Ser Gly Phe Asp Trp Val Thr Asp His Ser Gly Lys Thr Val Trp
1850 1855 1860
Phe Val Pro Ser Val Arg Asn Gly Asn Glu Ile Ala Ala Cys Leu
1865 1870 1875
Thr Lys Ala Gly Lys Arg Val Ile Gln Leu Ser Arg Lys Thr Phe
1880 1885 1890
Glu Thr Glu Phe Gln Lys Thr Lys His Gln Glu Trp Asp Phe Val
1895 1900 1905
Val Thr Thr Asp Ile Ser Glu Met Gly Ala Asn Phe Lys Ala Asp
1910 1915 1920
Arg Val Ile Asp Ser Arg Arg Cys Leu Lys Pro Val Ile Leu Asp
1925 1930 1935
Gly Glu Arg Val Ile Leu Ala Gly Pro Met Pro Val Thr His Ala
1940 1945 1950
Ser Ala Ala Gln Arg Arg Gly Arg Ile Gly Arg Asn Pro Asn Lys
1955 1960 1965
Pro Gly Asp Glu Tyr Leu Tyr Gly Gly Gly Cys Ala Glu Thr Asp
1970 1975 1980
Glu Asp His Ala His Trp Leu Glu Ala Arg Met Leu Leu Asp Asn
1985 1990 1995
Ile Tyr Leu Gln Asp Gly Leu Ile Ala Ser Leu Tyr Arg Pro Glu
2000 2005 2010
Ala Asp Lys Val Ala Ala Ile Glu Gly Glu Phe Lys Leu Arg Thr
2015 2020 2025
Glu Gln Arg Lys Thr Phe Val Glu Leu Met Lys Arg Gly Asp Leu
2030 2035 2040
Pro Val Trp Leu Ala Tyr Gln Val Ala Ser Ala Gly Ile Thr Tyr
2045 2050 2055
Thr Asp Arg Arg Trp Cys Phe Asp Gly Thr Thr Asn Asn Thr Ile
2060 2065 2070
Met Glu Asp Ser Val Pro Ala Glu Val Trp Thr Arg His Gly Glu
2075 2080 2085
Lys Arg Val Leu Lys Pro Arg Trp Met Asp Ala Arg Val Cys Ser
2090 2095 2100
Asp His Ala Ala Leu Lys Ser Phe Lys Glu Phe Ala Ala Gly Lys
2105 2110 2115
Arg Gly Ala Ala Phe Gly Val Met Glu Ala Leu Gly Thr Leu Pro
2120 2125 2130
Gly His Met Thr Glu Arg Phe Gln Glu Ala Ile Asp Asn Leu Ala
2135 2140 2145
Val Leu Met Arg Ala Glu Thr Gly Ser Arg Pro Tyr Lys Ala Ala
2150 2155 2160
Ala Ala Gln Leu Pro Glu Thr Leu Glu Thr Ile Met Leu Leu Gly
2165 2170 2175
Leu Leu Gly Thr Val Ser Leu Gly Ile Phe Phe Val Leu Met Arg
2180 2185 2190
Asn Lys Gly Ile Gly Lys Met Gly Phe Gly Met Val Thr Leu Gly
2195 2200 2205
Ala Ser Ala Trp Leu Met Trp Leu Ser Glu Ile Glu Pro Ala Arg
2210 2215 2220
Ile Ala Cys Val Leu Ile Val Val Phe Leu Leu Leu Val Val Leu
2225 2230 2235
Ile Pro Glu Pro Glu Lys Gln Arg Ser Pro Gln Asp Asn Gln Met
2240 2245 2250
Ala Ile Ile Ile Met Val Ala Val Gly Leu Leu Gly Leu Ile Thr
2255 2260 2265
Ala Asn Glu Leu Gly Trp Leu Glu Arg Thr Lys Ser Asp Leu Ser
2270 2275 2280
His Leu Met Gly Arg Arg Glu Glu Gly Ala Thr Ile Gly Phe Ser
2285 2290 2295
Met Asp Ile Asp Leu Arg Pro Ala Ser Ala Trp Ala Ile Tyr Ala
2300 2305 2310
Ala Leu Thr Thr Phe Ile Thr Pro Ala Val Gln His Ala Val Thr
2315 2320 2325
Thr Ser Tyr Asn Asn Tyr Ser Leu Met Ala Met Ala Thr Gln Ala
2330 2335 2340
Gly Val Leu Phe Gly Met Gly Lys Gly Met Pro Phe Tyr Ala Trp
2345 2350 2355
Asp Phe Gly Val Pro Leu Leu Met Ile Gly Cys Tyr Ser Gln Leu
2360 2365 2370
Thr Pro Leu Thr Leu Ile Val Ala Ile Ile Leu Leu Val Ala His
2375 2380 2385
Tyr Met Tyr Leu Ile Pro Gly Leu Gln Ala Ala Ala Ala Arg Ala
2390 2395 2400
Ala Gln Lys Arg Thr Ala Ala Gly Ile Met Lys Asn Pro Val Val
2405 2410 2415
Asp Gly Ile Val Val Thr Asp Ile Asp Thr Met Thr Ile Asp Pro
2420 2425 2430
Gln Val Glu Lys Lys Met Gly Gln Val Leu Leu Ile Ala Val Ala
2435 2440 2445
Val Ser Ser Ala Ile Leu Ser Arg Thr Ala Trp Gly Trp Gly Glu
2450 2455 2460
Ala Gly Ala Leu Ile Thr Ala Ala Thr Ser Thr Leu Trp Glu Gly
2465 2470 2475
Ser Pro Asn Lys Tyr Trp Asn Ser Ser Thr Ala Thr Ser Leu Cys
2480 2485 2490
Asn Ile Phe Arg Gly Ser Tyr Leu Ala Gly Ala Ser Leu Ile Tyr
2495 2500 2505
Thr Val Thr Arg Asn Ala Gly Leu Val Lys Arg Arg Gly Gly Gly
2510 2515 2520
Thr Gly Glu Thr Leu Gly Glu Lys Trp Lys Ala Arg Leu Asn Gln
2525 2530 2535
Met Ser Ala Leu Glu Phe Tyr Ser Tyr Lys Lys Ser Gly Ile Thr
2540 2545 2550
Glu Val Cys Arg Glu Glu Ala Arg Arg Ala Leu Lys Asp Gly Val
2555 2560 2565
Ala Thr Gly Gly His Ala Val Ser Arg Gly Ser Ala Lys Leu Arg
2570 2575 2580
Trp Leu Val Glu Arg Gly Tyr Leu Gln Pro Tyr Gly Lys Val Ile
2585 2590 2595
Asp Leu Gly Cys Gly Arg Gly Gly Trp Ser Tyr Tyr Ala Ala Thr
2600 2605 2610
Ile Arg Lys Val Gln Glu Val Lys Gly Tyr Thr Lys Gly Gly Pro
2615 2620 2625
Gly His Glu Glu Pro Met Leu Val Gln Ser Tyr Gly Trp Asn Ile
2630 2635 2640
Val Arg Leu Lys Ser Gly Val Asp Val Phe His Met Ala Ala Glu
2645 2650 2655
Pro Cys Asp Thr Leu Leu Cys Asp Ile Gly Glu Ser Ser Ser Ser
2660 2665 2670
Pro Glu Val Glu Glu Ala Arg Thr Leu Arg Val Leu Ser Met Val
2675 2680 2685
Gly Asp Trp Leu Glu Lys Arg Pro Gly Ala Phe Cys Ile Lys Val
2690 2695 2700
Leu Cys Pro Tyr Thr Ser Thr Met Met Glu Thr Leu Glu Arg Leu
2705 2710 2715
Gln Arg Arg Tyr Gly Gly Gly Leu Val Arg Val Pro Leu Ser Arg
2720 2725 2730
Asn Ser Thr His Glu Met Tyr Trp Val Ser Gly Ala Lys Ser Asn
2735 2740 2745
Thr Ile Lys Ser Val Ser Thr Thr Ser Gln Leu Leu Leu Gly Arg
2750 2755 2760
Met Asp Gly Pro Arg Arg Pro Val Lys Tyr Glu Glu Asp Val Asn
2765 2770 2775
Leu Gly Ser Gly Thr Arg Ala Val Val Ser Cys Ala Glu Ala Pro
2780 2785 2790
Asn Met Lys Ile Ile Gly Asn Arg Ile Glu Arg Ile Arg Ser Glu
2795 2800 2805
His Ala Glu Thr Trp Phe Phe Asp Glu Asn His Pro Tyr Arg Thr
2810 2815 2820
Trp Ala Tyr His Gly Ser Tyr Glu Ala Pro Thr Gln Gly Ser Ala
2825 2830 2835
Ser Ser Leu Ile Asn Gly Val Val Arg Leu Leu Ser Lys Pro Trp
2840 2845 2850
Asp Val Val Thr Gly Val Thr Gly Ile Ala Met Thr Asp Thr Thr
2855 2860 2865
Pro Tyr Gly Gln Gln Arg Val Phe Lys Glu Lys Val Asp Thr Arg
2870 2875 2880
Val Pro Asp Pro Gln Glu Gly Thr Arg Gln Val Met Ser Met Val
2885 2890 2895
Ser Ser Trp Leu Trp Lys Glu Leu Gly Lys His Lys Arg Pro Arg
2900 2905 2910
Val Cys Thr Lys Glu Glu Phe Ile Asn Lys Val Arg Ser Asn Ala
2915 2920 2925
Ala Leu Gly Ala Ile Phe Glu Glu Glu Lys Glu Trp Lys Thr Ala
2930 2935 2940
Val Glu Ala Val Asn Asp Pro Arg Phe Trp Ala Leu Val Asp Lys
2945 2950 2955
Glu Arg Glu His His Leu Arg Gly Glu Cys Gln Ser Cys Val Tyr
2960 2965 2970
Asn Met Met Gly Lys Arg Glu Lys Lys Gln Gly Glu Phe Gly Lys
2975 2980 2985
Ala Lys Gly Ser Arg Ala Ile Trp Tyr Met Trp Leu Gly Ala Arg
2990 2995 3000
Phe Leu Glu Phe Glu Ala Leu Gly Phe Leu Asn Glu Asp His Trp
3005 3010 3015
Met Gly Arg Glu Asn Ser Gly Gly Gly Val Glu Gly Leu Gly Leu
3020 3025 3030
Gln Arg Leu Gly Tyr Val Leu Glu Glu Met Ser Arg Ile Pro Gly
3035 3040 3045
Gly Arg Met Tyr Ala Asp Asp Thr Ala Gly Trp Asp Thr Arg Ile
3050 3055 3060
Ser Arg Phe Asp Leu Glu Asn Glu Ala Leu Ile Thr Asn Gln Met
3065 3070 3075
Glu Lys Gly His Arg Ala Leu Ala Leu Ala Ile Ile Lys Tyr Thr
3080 3085 3090
Tyr Gln Asn Lys Val Val Lys Val Leu Arg Pro Ala Glu Lys Gly
3095 3100 3105
Lys Thr Val Met Asp Ile Ile Ser Arg Gln Asp Gln Arg Gly Ser
3110 3115 3120
Gly Gln Val Val Thr Tyr Ala Leu Asn Thr Phe Thr Asn Leu Val
3125 3130 3135
Val Gln Leu Ile Arg Asn Met Glu Ala Glu Glu Val Leu Glu Met
3140 3145 3150
Gln Asp Leu Trp Leu Leu Arg Arg Ser Glu Lys Val Thr Asn Trp
3155 3160 3165
Leu Gln Ser Asn Gly Trp Asp Arg Leu Lys Arg Met Ala Val Ser
3170 3175 3180
Gly Asp Asp Cys Val Val Lys Pro Ile Asp Asp Arg Phe Ala His
3185 3190 3195
Ala Leu Arg Phe Leu Asn Asp Met Gly Lys Val Arg Lys Asp Thr
3200 3205 3210
Gln Glu Trp Lys Pro Ser Thr Gly Trp Asp Asn Trp Glu Glu Val
3215 3220 3225
Pro Phe Cys Ser His His Phe Asn Lys Leu His Leu Lys Asp Gly
3230 3235 3240
Arg Ser Ile Val Val Pro Cys Arg His Gln Asp Glu Leu Ile Gly
3245 3250 3255
Arg Ala Arg Val Ser Pro Gly Ala Gly Trp Ser Ile Arg Glu Thr
3260 3265 3270
Ala Cys Leu Ala Lys Ser Tyr Ala Gln Met Trp Gln Leu Leu Tyr
3275 3280 3285
Phe His Arg Arg Asp Leu Arg Leu Met Ala Asn Ala Ile Cys Ser
3290 3295 3300
Ser Val Pro Val Asp Trp Val Pro Thr Gly Arg Thr Thr Trp Ser
3305 3310 3315
Ile His Gly Lys Gly Glu Trp Met Thr Thr Glu Asp Met Leu Val
3320 3325 3330
Val Trp Asn Arg Val Trp Ile Glu Glu Asn Asp His Met Glu Asp
3335 3340 3345
Lys Thr Pro Val Thr Lys Trp Thr Asp Ile Pro Tyr Leu Gly Lys
3350 3355 3360
Arg Glu Asp Leu Trp Cys Gly Ser Leu Ile Gly His Arg Pro Arg
3365 3370 3375
Thr Thr Trp Ala Glu Asn Ile Lys Asn Thr Val Asn Met Val Arg
3380 3385 3390
Arg Ile Ile Gly Asp Glu Glu Lys Tyr Met Asp Tyr Leu Ser Thr
3395 3400 3405
Gln Val Arg Tyr Leu Gly Glu Glu Gly Ser Thr Pro Gly Val Leu
3410 3415 3420
<210> 74
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 74
ttaggatccg ttgttgatct gtgtgaat 28
<210> 75
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 75
taactcgagc gtacacaacc caagtt 26
<210> 76
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 76
ttaggatcct cactagacgt gggagtg 27
<210> 77
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 77
taactcgaga agccatgtcy gatattgat 29
<210> 78
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 78
ttaggatccg catacagcat caggtg 26
<210> 79
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 79
taactcgagt gtggagttcc ggtgtct 27
<210> 80
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 80
ttaggatccg aatagagcga argttgagat a 31
<210> 81
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 81
taactcgagt ggtgggtgat cttcttct 28
<210> 82
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 82
ttaggatcca gtcacagtgg aggtacagta c 31
<210> 83
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 83
taactcgagc rcagatacca tcttccc 27
<210> 84
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 84
ttaggatccc ttatgtgctt ggccttag 28
<210> 85
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 85
taactcgagt cttcagcctc catgtg 26
<210> 86
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 86
ttaggatcca atgcccactc aaacataga 29
<210> 87
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 87
taactcgagt cattctcttc ttcagccctt 30
<210> 88
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 88
ttaggatcca agggtgatcg aggaat 26
<210> 89
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 89
taactcgagt tcccttcaga gagaggagc 29
<210> 90
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 90
ttaggatcct cttttgcaaa ctgcgatc 28
<210> 91
<211> 27
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 91
taactcgagt ccagctgcaa agggtat 27
<210> 92
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 92
ttaggatccg tgtggacatg tacattga 28
<210> 93
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 93
taactcgagc ccattgccat aaagtc 26
<210> 94
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 94
ttaggatcct catactgtgg tccatgga 28
<210> 95
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 95
taactcgagg cccatctcaa cccttg 26
<210> 96
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 96
ttaggatcct agagggcttc cagtgc 26
<210> 97
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 97
taactcgaga tactcatctc caggtttgtt g 31
<210> 98
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 98
ttaggatccg aaaacaaaac atcaagagtg 30
<210> 99
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 99
taactcgagg aatctctctg tcatgtgtcc t 31
<210> 100
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 100
ttaggatcct tgatggcacg accaac 26
<210> 101
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 101
ttaggatccg ttgttgatct gtgtgaat 28
<210> 102
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 102
taactcgagc aggtcaatgt ccattg 26
<210> 103
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 103
ttaggatcct gttgtgttcc tattgctggt 30
<210> 104
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 104
taactcgagt gatcagrgcc ccagc 25
<210> 105
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 105
ttaggatcct gctgcccaga agagaa 26
<210> 106
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 106
taactcgagc accaacaygg gttctt 26
<210> 107
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 107
ttaggatcct caaggacggt gtggc 25
<210> 108
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 108
taactcgagc aatgatcttc atgttggg 28
<210> 109
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 109
ttaggatcct atgggggagg actggt 26
<210> 110
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 110
taactcgagc ccagaacctt ggatc 25
<210> 111
<211> 25
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 111
ttaggatcca gacccccaag aaggc 25
<210> 112
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 112
taactcgagc ccctttggtc ttgtct 26
<210> 113
<211> 29
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 113
ttaggatcca ggaaggatgt atgcagatg 29
<210> 114
<211> 30
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 114
taactcgaga catttgcgca tatgattttg 30
<210> 115
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 115
ttaggatcca ggaaggacac acaagagt 28
<210> 116
<211> 26
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 116
taactcgaga caggctgcac agcttt 26
<210> 117
<211> 28
<212> DNA
<213> 人工序列
<220>
<223> 引物
<400> 117
ttaggatcct ctctcatagg gcacagac 28

Claims (17)

1.一种包含灭活Zika病毒颗粒的Zika病毒疫苗，其中，所述Zika病毒疫苗能够对至少70％的施用所述Zika病毒疫苗的受试者赋予血清保护。
2.根据权利要求1所述的Zika病毒，其中，所述Zika病毒颗粒能够对至少75％、80％、90％、95％、96％、97％、98％或至少99％的施用Zika病毒疫苗的接种疫苗受试者赋予血清保护，优选对至少80％的受试者赋予血清保护。
3.根据权利要求1或2所述的疫苗，其中，所述Zika病毒颗粒具有对应于由SEQ ID NO：2-13或72的核酸序列中的任一个提供的DNA序列的RNA基因组、或与SEQ ID NO：2-13或72中的任一个至少88％相同并且能够包裹毒性Zika病毒的变体核酸序列。
4.根据权利要求1至3中任一项所述的疫苗，其中，所述Zika病毒颗粒具有选自由SEQID NO：14-69中的任一个提供的氨基酸序列中的E蛋白、或与SEQ ID NO：14-69中的任一个至少95％相同并且能够包裹毒性Zika病毒的变体氨基酸序列。
5.根据权利要求1至4中任一项所述的疫苗，其中，所述Zika病毒通过化学灭活、热灭活、pH灭活或UV灭活来灭活。
6.根据权利要求5所述的疫苗，其中，所述化学灭活包括：使Zika病毒与化学灭活剂接触的时间比通过斑块测定所测量的完全灭活Zika病毒所需的更久。
7.根据权利要求6所述的疫苗，其中，所述化学灭活包括使Zika病毒与甲醛接触。
8.根据权利要求7所述的疫苗，其中，所述甲醛灭活包括使Zika病毒与甲醛接触2-10天。
9.根据权利要求5至8中任一项所述的疫苗，其中，所述化学活化在约+4℃或约+22℃进行。
10.根据权利要求1至9中任一项所述的疫苗，其进一步包含佐剂。
11.根据权利要求10所述的疫苗，其中，所述佐剂是铝盐佐剂。
12.根据权利要求11所述的疫苗，其中，所述铝盐佐剂是氢氧化铝或磷酸铝盐。
13.根据权利要求10至12中任一项所述的疫苗，其中，所述疫苗包含或进一步包含：含有肽和含有脱氧肌苷的免疫刺激性寡聚脱氧核酸分子(I-ODN)的佐剂。
14.根据权利要求13所述的疫苗，其中，所述肽包含序列KLKL5KLK(SEQ ID NO：71)，并且I-ODN包含寡聚-d(IC)13(SEQ ID NO：70)。
15.根据权利要求1至14中任一项所述的疫苗，其还包含一种或多种药学上可接受的赋形剂。
16.根据权利要求1至15中任一项所述的疫苗，其中，所述疫苗含有硫酸鱼精蛋白或者PS的片段或分解产物，其含量太低而不能被HPLC检测出，即低于1μg/mL，尤其低于100ng/mL。
17.根据权利要求16所述的疫苗，其中所述硫酸鱼精蛋白或者PS的片段或分解产物能够通过质谱或其它敏感方法检测。
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Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9895437B2 (en) 2012-04-18 2018-02-20 Valneva Austria Gmbh Aluminum compounds for use in therapeutics and vaccines
EP3322441A4 (en) * 2015-07-16 2018-12-19 Bharat Biotech International Limited Vaccine compositions
PL3393510T3 (pl) * 2015-12-23 2023-06-26 Valneva Austria Gmbh Szczepionka przeciwko wirusowi zika
CA3030154A1 (en) * 2016-07-08 2018-01-11 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Chimeric dengue/zika viruses live-attenuated zika virus vaccines
WO2019057793A1 (en) 2017-09-21 2019-03-28 Valneva Se PROCESS FOR THE PRODUCTION OF PHARMACEUTICAL COMPOSITIONS COMPRISING THE IMMUNOGENIC VIRUS OF CHIKUNGUNYA CHIKV-DELTA5NSP3
EP3703741A1 (en) * 2017-11-03 2020-09-09 Takeda Vaccines, Inc. Zika vaccines and immunogenic compositions, and methods of using the same
CN107988239B (zh) * 2017-11-29 2021-03-19 南方医科大学 一种寨卡病毒的重组基因及其制备方法和应用
BR112020009960A2 (pt) * 2017-11-30 2020-11-10 Takeda Vaccines, Inc. método para a inativação de vírus zika e métodos relacionados
CN107904215B (zh) * 2017-12-27 2021-07-27 华农（肇庆）生物产业技术研究院有限公司 一种禽流感病毒的全悬浮培养方法
CN110156880B (zh) * 2018-02-13 2022-12-16 吴夙钦 兹卡病毒疫苗组合物及其应用
TWI658848B (zh) * 2018-02-13 2019-05-11 國立清華大學 茲卡病毒疫苗組合物及其應用
AU2019242601A1 (en) * 2018-03-26 2020-11-12 Boehringer Ingelheim Animal Health USA Inc. Method of producing an immunogenic composition
CN108676780B (zh) * 2018-07-24 2021-08-10 华中农业大学 一种高效分离猪肠道冠状病毒的方法
CN109337876A (zh) * 2018-10-15 2019-02-15 北京世纪元亨动物防疫技术有限公司 一种猪流行性腹泻病毒的纯化方法
EP3883961A1 (en) 2018-11-20 2021-09-29 Takeda Vaccines, Inc. Novel anti-zika virus antibodies and uses thereof
CN109627297B (zh) * 2018-12-29 2022-06-14 复旦大学 来自寨卡病毒e蛋白的中和表位及其应用
AU2020211990A1 (en) * 2019-01-22 2021-08-12 2Seventy Bio, Inc. Methods and systems for manufacturing viral vectors
AU2020269164B2 (en) * 2019-05-08 2024-04-04 Takeda Vaccines, Inc. Inactivated virus compositions and Zika vaccine formulations
KR20220045154A (ko) 2019-08-09 2022-04-12 발네바 에스이 치쿤구니야 백신 제형
BR112022002110A2 (pt) 2019-08-09 2022-04-19 Valneva Se Vacina do vírus da chikungunya de dose única
WO2021048221A1 (en) 2019-09-09 2021-03-18 Valneva Austria Gmbh Inactivation process for viruses
KR102243295B1 (ko) * 2019-11-15 2021-04-23 강원대학교산학협력단 지카바이러스의 외피 단백질을 포함하는 재조합 백신 조성물
KR102365464B1 (ko) * 2019-12-24 2022-02-22 강원대학교산학협력단 지카바이러스 재조합 서브유닛 백신의 개발 및 이의 제조방법
WO2021158815A1 (en) * 2020-02-05 2021-08-12 New York Blood Center, Inc. Zika virus immunogenic compositions
GB2592769B (en) 2020-03-01 2022-05-04 Valneva Austria Gmbh CpG-Adjuvanted SARS-CoV-2 virus vaccine
EP3895729A1 (en) 2020-03-01 2021-10-20 Valneva Austria GmbH Cpg-adjuvanted sars-cov-2 virus vaccine
EP4114844A4 (en) * 2020-03-03 2024-04-10 Mayo Found Medical Education & Res ZIKA VIRUS POLYPEPTIDES
UY39159A (es) 2020-04-06 2021-10-29 Valneva Austria Gmbh VACUNA INACTIVADA CONTRA EL VIRUS DEL SARS-CoV-2
EP4153222A1 (en) 2020-05-20 2023-03-29 Takeda Vaccines, Inc. Method for detection of zika virus specific antibodies
WO2021236225A1 (en) 2020-05-20 2021-11-25 Takeda Vaccines, Inc. Method for detection of zika virus specific antibodies
EP4154003A1 (en) 2020-05-20 2023-03-29 Takeda Vaccines, Inc. Method for determining the potency of antigens
WO2023148256A1 (en) 2022-02-02 2023-08-10 Valneva Austria Gmbh Inactivated sars-cov-2 virus vaccine

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US86061A (en) * 1869-01-19 Improved horse-power
DK1604685T3 (da) 1997-08-28 2012-06-18 Cj Cheiljedang Corp Et svækket japansk encephalitisvirus
ATE471983T1 (de) * 1998-09-02 2010-07-15 Us Gov Health & Human Serv Dengue viren, die in mosquitos nicht replizieren, zur verwendung als impfstoff
DE60135983D1 (de) 2000-05-31 2008-11-13 Novartis Vaccines & Diagnostic Verfahren zur reinigung von alphaviralen replikon partikeln
WO2002074963A1 (en) * 2001-03-16 2002-09-26 The Government Of The United States Of America, As Represented By The Secretary, Department Of Healt Dengue viruses that are replication defective in mosquitos for use as vaccines
US7871814B2 (en) * 2001-05-10 2011-01-18 The Regents Of The University Of California Recombinant bicistronic flaviviruses and methods of use thereof
AU2004224746B2 (en) 2003-03-24 2009-04-23 Valneva Austria Gmbh Improved vaccines
US8865184B2 (en) * 2006-09-01 2014-10-21 Bharat Biotech International Limited Vaccine for chikungunya virus infection
WO2009048633A2 (en) 2007-10-11 2009-04-16 The Board Of Regents Of The University Of Texas System Chimeric chikungunya virus and uses thereof
JP5773366B2 (ja) * 2008-04-21 2015-09-02 ティシュー リジェネレイション セラピューティックス、インコーポレイテッド 生物学的又は化学的作用物質に対する予防又はそれらの治療のために遺伝子改変されたヒト臍帯血管周囲細胞
CN102317308A (zh) * 2008-11-26 2012-01-11 美国国有健康与人类服务部 类病毒组合物颗粒及其使用方法
WO2010085358A2 (en) * 2009-01-23 2010-07-29 The Board Of Regents Of The University Of Texas System Flaviviruses expressing the prm, e, and ns1 proteins of other flaviviruses and uses thereof
WO2011101465A1 (en) 2010-02-19 2011-08-25 Intercell Ag Ic31 nanoparticles
EP3269386B1 (en) 2011-12-06 2022-09-14 Valneva Austria GmbH Aluminium compounds for use in therapeutics and vaccines
US9895437B2 (en) 2012-04-18 2018-02-20 Valneva Austria Gmbh Aluminum compounds for use in therapeutics and vaccines
WO2016145149A1 (en) * 2015-03-11 2016-09-15 The United States Of America, As Represented By The Secretary Of The Army, On Behalf Of The Walter Reed Army Institute Of Research Combination purified inactivated vaccine for flaviviruses
EP3322441A4 (en) * 2015-07-16 2018-12-19 Bharat Biotech International Limited Vaccine compositions
PL3393510T3 (pl) 2015-12-23 2023-06-26 Valneva Austria Gmbh Szczepionka przeciwko wirusowi zika
CN105749268B (zh) 2016-04-11 2020-09-11 北京科兴中维生物技术有限公司 一种灭活的寨卡病毒疫苗
WO2019057793A1 (en) 2017-09-21 2019-03-28 Valneva Se PROCESS FOR THE PRODUCTION OF PHARMACEUTICAL COMPOSITIONS COMPRISING THE IMMUNOGENIC VIRUS OF CHIKUNGUNYA CHIKV-DELTA5NSP3

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