KR20230005814A - Cpg-어쥬번트된 sars-cov-2 바이러스 백신 - Google Patents

Cpg-어쥬번트된 sars-cov-2 바이러스 백신 Download PDF

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KR20230005814A
KR20230005814A KR1020227034303A KR20227034303A KR20230005814A KR 20230005814 A KR20230005814 A KR 20230005814A KR 1020227034303 A KR1020227034303 A KR 1020227034303A KR 20227034303 A KR20227034303 A KR 20227034303A KR 20230005814 A KR20230005814 A KR 20230005814A
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안드레아스 메인케
미하엘 모렌
크리스토프 리이니시
로버트 슈레글
크리스티안 타우쳐
존 디. 캠벨
데이빗 노백
로버트 에스. 얀센
위르겐 하인들-루쓰
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발네바 오스트리아 게엠베하
다이나박스 테크놀로지 코퍼레이션
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Priority claimed from PCT/US2021/020313 external-priority patent/WO2021178318A1/en
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Priority claimed from PCT/IB2021/052858 external-priority patent/WO2021176434A1/en
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
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Abstract

CpG-어쥬번트된 SARS-CoV-2 백신과 조성물 및 상기 백신을 생산하고 이를 필요로 하는 대상체에게 투여하는 방법이 본 명세서에 기재되어 있다.

Description

CPG-어쥬번트된 SARS-COV-2 바이러스 백신
개시내용은 CpG-어쥬번트된 SARS-CoV-2 백신 및 상기 백신을 생산하고 항-SARS-CoV-2 면역 반응의 생성을 위해 대상체에게 백신을 투여하기 위한 조성물 및 방법에 관한 것이다.
SARS-CoV-2(이하 "바이러스")는 2019년 11월경 중국에서 처음으로 검출되었다. 그런 다음 이 바이러스는 세계적인 팬데믹을 일으켰다. 천연 보유숙주는 박쥐이고 바이러스는 코로나바이러스과 패밀리인, 베타코로나바이러스(betaCoV) 속에 속한다. 바이러스는 25개의 비-구조 단백질 및 4개의 구조 단백질을 포함한 9,860개 아미노산 다단백질을 인코딩하는 29,903bp(Wuhan-Hu-1: Genbank 참조 서열: NC_045512.2)로 구성된 ssRNA 게놈: 스파이크(S), 엔벨로프(E), 막(M), 뉴클레오캡시드(N) 단백질을 가지고 있다. 바이러스는 60 내지 140nm 사이의 다양한 직경을 가지고 있다. 그것은 엔벨롭되고 UV, 열 및 지질 용매에 민감하다. 그것은 박쥐 SARS-유사-CoVZXC21과 89% 뉴클레오티드 서열 동일성을 갖고 인간 SARS-CoV와 82% 뉴클레오티드 서열 동일성을 가지고 있다(Chan et al. 2020). 증거에 따르면 이 바이러스는 감염된 인간이 기침할 때 - 바이러스로 팩킹된 - 작은 비말을 공기 중으로 퍼트린다. 이것들은 숨을 들이쉬거나 닿은 표면을 만진 다음 눈, 코 또는 입을 만지면 감염을 일으킬 수 있다. 부가하여, 다른 병원 매개체가 존재할 수 있고 바이러스는 수혈에 의해, 태반으로 그리고 성적 전달을 통해 전달될 수 있다. SARS-CoV-2 바이러스로 감염을 통해 단지 가벼운 증상, 예컨대 전형적으로 발열과 기침을 초래할 수 있거나 더욱이 무증상일 수 있으며; 다른 극단에서는 치명적일 수 있다. 주요 증상은 일반적으로 고열, 기침 및 호흡 곤란이다. 현재로 이 바이러스에 대한 특정 치료법이나 백신은 없고 유일한 예방 방법은 사회적 거리두기를 포함한다. SARS-CoV-2는 상당한 공중 보건 위협을 나타낸다. Imperial College COVID-19(SARS-CoV-2로 인한 질환) 대응팀은 2020년 3월 16일에, 궁극적으로 의료 시스템의 붕괴와 영국에서만 수십만 명의 사망을 초래한 바이러스의 확산을 막거나 지연시키기 위해 이용가능한 가능한 모든 방법을 평가한 보고서를 발표하였다. 보고서는 인구 전반에 걸친 사회적 거리두기만이 효과를 관리가능한 수준으로 줄일 수 있는 기회를 가지고 백신이 이용가능할 때까지 이들 조치를 따라야 한다고 언급했다. 이 권장사항은 대부분의 인구가 최소 18개월 동안 격리됨을 의미한다. 보고서는 대량-생산가능한 백신이 노인 인구를 기꺼이 희생하는 것 외에 이 팬데믹을 중단하는 유일한 옵션이라고 결론지었다. 극적인 상황을 고려할 때, SARS-CoV-2에 대한 효과적인 백신이 가능한 한 빨리 절대적인 긴급한 필요성이 있다. 더욱이, 다양한 탈출 돌연변이(예를 들어, UK_B.1.1.7; South African_B.1.351; Californian_B.1.427/B.1.429 및 Brazil_P.1 변종, 도 2 참조)가 출현하여 상황을 더욱 악화시켰고 따라서 이 불행한 전개도 해결되어야할 필요가 있다.
발명의 요약
따라서, 본 발명은 불활성화된 전체 바이러스 SARS-CoV-2 백신을 제공한다. 벡터화 백신, 전체-비리온 불활성화된 백신, 및 재조합 단백질 백신을 포함한 다수의 SARS-CoV-2 백신이 개발 중에 있다. mRNA 또는 DNA 백신 또는 벡터화 백신 후보가 어쥬번트 없이 T 세포 반응을 유도하지만, 어쥬번트는 서브유닛 및 불활성화된 백신이 그 면역원성을 증가시키는 데 중요할 수 있다. 더욱이, 신속한 개발 과정에서 가장 큰 도전과제는 사려깊은 백신 설계와 시기적절한 철저한 평가 둘 모두에 의해 안전성 문제를 피하는 것이다. SARS-CoV-2 백신과 관련하여 잠재적인 면역-매개된 질환 증강과 관련하여 안전성 우려가 제기되었다. 명반-어쥬번트된 전체 바이러스 불활화된 백신 후보를 포함하여 SARS-CoV-1 백신 후보에 대한 다수의 연구에서 살아있는 바이러스로 공격 후 백신접종한 동물에서 질환 증강에 대한 증거가 있다. 마우스에서 SARS-CoV-1에 의해 유도된 면역병리는 백신 항원에 대한 우성 Th2 유형 반응의 결과로 간주되었다(Tseng C-T et al. 2012 Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus. PlosOne 7(4):e35421). 이것은 Th1에 대한 면역 반응을 유도하는 것으로 알려진 백신 또는 다른 백신 제형에 다른 어쥬번트(예를 들어 CpG)를 포함시킨 후에는 관찰되지 않았다. 불활성화된 백신 접근법이 고려되는 한, 표준 조건 하에서 전형적인 불활성화제(예를 들어 포름알데히드)의 사용은 특히 천연 에피토프의 파괴와 같은 결점을 가질 수 있으며, 이는 효과적인 백신 후보의 개발을 방해한다. 본 발명은 이들 문제를 해결하고 따라서 종래 기술의 단점을 극복한 안전하고 효과적인 전체-바이러스 불활성화된 SARS-CoV-2 백신을 생산하는 것을 목적으로 한다.
따라서, 일 양태에서, 본 발명은 시티딘-포스포-구아노신(CpG) 및 명반 어쥬번트화와 조합된 불활성화된 SARS-CoV-2 입자를 포함하는 SARS-CoV-2 백신을 제공한다. 상기에서 언급한 바와 같이, SARS-CoV-2 백신에 대한 적절한 어쥬번트 또는 어쥬번트들의 선택이 매우 중요한 것일 수 있다. 명반의 사용이 인간에서 Th2 왜곡을 반드시 유발하지는 않을 수 있지만 CpG의 추가는 가능한 백신-관련된 질환 증강 안전성 우려를 완화하는 것으로 믿어진다. CpG의 추가는 더욱이 SARS-CoV-2 백신에 대한 긴급한 세계적 요구에 비추어 볼 때 또 다른 중요한 고려사항인 대상체에서 혈청전환을 달성하는 데 필요한 항원 용량의 상당한 감소(즉, "용량 절약")를 허용할 수 있다. 마지막으로, 어쥬번트의 추가는 SARS-CoV-2 이환율 또는 사망률에 특히 민감하거나 취약한 대상체, 즉 면역저하, 임신 또는 노인 대상체에서 강력한 면역 반응을 생성하는 데 도움이 될 수 있다. 이러한 백신 조성물은 하기에 더 상세히 기술되어 있다.
바람직한 양태에서, 백신에서 불활성화된 SARS-CoV-2 입자의 표면은 백신이 인간 대상체에서 천연 SARS-CoV-2 입자에 대한 중화 항체를 생성할 수 있도록 천연 형태를 나타낸다. 특히, 본 발명은 인간 세포의 복제 및 감염이 불가능하지만 바이러스 표면 단백질의 면역원성 에피토프를 보유하고 따라서 백신접종된 대상체에서 보호 면역을 생성하기에 적합한 최적으로 불활성화된 SARS-CoV-2 입자를 제공하는 것을 목적으로 한다. 불활성화 과정(예를 들어 베타-프로피오락톤 사용) 및 적절한 어쥬번트의 선택을 포함하여 백신의 생산에서 기타 단계를 최적화함에 의해, SARS-CoV-2 입자의 고유 표면 형태를 보존하고 백신 유도된 면역병리 또는 질환의 증강의 위험을 감소시키는 새로운 백신 조성물을 얻을 수 있다. 따라서, 일 양태에서, SARS-CoV-2 백신 조성물은 베타-프로피오락톤-불활성화된 SARS-CoV-2 입자를 포함하고, 여기서 백신은 인간 대상체에서 천연 SARS-CoV-2 입자에 대한 중화 항체를 생성할 수 있으며, 바람직하게는 여기서 SARS-CoV-2 입자의 천연 표면 형태는 백신에서 보존된다.
추가의 특정 양태에서, 발명은 인간 세포의 복제 및 감염이 불가능하지만 바이러스 표면 단백질의 면역원성 에피토프를 보유하고 따라서 백신접종된 대상체에서 보호 면역을 생성하는 데 적합한 최적으로 불활성화된(예를 들어, 베타-프로피오락톤-불활성화된) SARS-CoV-2 입자의 최적 조합을 제공하는 것을 목적으로 한다. 명반 및 CpG 어쥬번트화와 조합하여 또한 불활성화된 SARS-CoV-2 입자의 최적의 조합에 의해, 상기 백신접종된 인간 대상체의 50% 초과, 바람직하게는 60% 초과, 70% 초과, 80% 초과, 90% 초과를 부분적으로 또는 완전히 보호할 수 있는 인간 대상체에서 천연 SARS-CoV-2 입자 및/또는 기타 면역학적 반응에 대한 중화 항체를 생성할 수 있는 개선된 백신 조성물을 얻을 수 있다.
발명의 각각의 제한은 발명의 다양한 실시형태를 포괄할 수 있다. 따라서 임의의 하나의 요소 또는 요소들의 조합을 포함하는 발명의 각각의 제한이 발명의 각각의 양태에 포함될 수 있음이 예상된다. 본 발명은 다음의 설명에 제시되거나 도면에 예시된 구성요소의 배열 및 구성의 세부사항에 대한 적용에 제한되지 않는다. 발명은 다른 실시형태가 가능하고 다양한 방식으로 실시되거나 수행될 수 있다.
첨부되는 도면은 축척에 맞게 그려진 것으로 의도되지 않는다. 도면은 단지 예시적이고 개시내용을 가능하게 하기 위해 요구되지는 않는다. 명확성을 위해 모든 구성요소가 모든 도면에 표지되지 않을 수 있다. 도면에서:
도 1. 본 발명의 불활성화된 SARS-CoV-2 백신의 생산에 대한 공정. 단계는 Vero 숙주 세포의 세포 구축, SARS-CoV-2로 Vero 세포의 감염, 바이러스 수확, DNA 감소, 일차 불활성화, 정제, 선택적 2차 불활성화 및 어쥬번트로 제형화를 포함한다. 바이러스는 수산화알루미늄과 함께 또는 없이 제형화될 수 있다. CpG 어쥬번트는 바이러스 (및 선택적 명반)와 동일한 바이알에 포함되거나 투여 전에 바이러스와 혼합될 수 있다.
도 2. SARS-CoV-2 팬데믹의 경과 동안, UK B 1.1.7, 브라질 P1, 캘리포니아 B.1.427/B.1.429 및 남아프리카 B.1.351 계통과 같은 최근의 새로운 변이체 또는 계통을 포함하여 전 세계로부터의 단리물로부터 SARS-CoV-2 게놈 서열이 보고되었다. 완전한 SARS-CoV-2 게놈 서열의 수탁 번호와 기원은, 이용가능한 경우(- 또는 항목 없음 = 이용 불가), 상응하는 orf1ab 다단백질 및 S 단백질에 대한 수탁 번호와 함께 표의 형식으로 제공된다.
도 3. 발명의 SARS-CoV-2 백신에 대한 폴리싱 단계로 사용되는 수크로스 구배 원심분리를 위한 바람직한 설정.
도 4. SARS-CoV-2 백신에 반응한 총 IgG. 코팅 항원: S1(A), 스파이크 단백질(B) 및 핵단백질(C)의 수용체 결합 도메인. 평가변수 역가: 컷-오프로 사용된 3-배 블랭크의 흡광도(점선).
도 5. 명반 단독 및 명반/CpG 1018로 아쥬번트된 SARS-CoV-2 백신에 반응한 IgG1 및 IgG2a 역가. S1 단백질에 특이적인 항체 역가는 ELISA에 의해 결정되었다. 농도는 mAb 서브클래스 표준 곡선과의 비교에 의해 결정되었다.
도 6. SARS-CoV-2 백신에 대한 중화 역가. 시험된 마우스 혈청을 d35에 수집하였다. 명반/CpG 1018의 존재에서 중화 반응은 SARS-CoV-2에 양성인 회복기 공여자로부터의 혈장 범위에 있는 것으로 관찰되었다(NIBSC 20/162; 3명의 공여자로부터의 풀링된 혈청).
도 7. 생성 공정은 고밀도의 온전한 스파이크 단백질을 제공한다. 실시예 1에 따라 생성된 SARS-CoV-2 불활성화된 약물 물질의 전자 현미경 사진이 도시된다. AU당 약 1-1.5 107 바이러스 입자.
도 8. SARS-CoV-2 및 JEV 약물 물질의 크기-배제-크로마토그래피 및 SDS-PAGE 프로필의 비교. SDS-PAGE에 따른 고순도(>95%)(은 염색, 환원됨) 및 SE-HPLC에 따른 단량체 바이러스(>95%). 상이한 바이러스 입자 크기(JEV(IXIARO) 약 50nm, SARS-CoV2 약 100nm)에 기인한 머무름 시간에서의 차이.
도 9. NHP 챌린지 연구를 위한 연구 설계. 각각 8마리 동물의 3개 그룹; SARS-CoV-2 백신에 대한 2개 용량 그룹(10 AU & 40 AU, 투여 직전에 추가된 용량당 0.5mg/용량 Al3+ 및 1mg CpG 1018로 제형화됨) 및 위약 그룹(DPBS). SARS-CoV-2 챌린지 균주는 BetaCoV/France/IDF/0372/2020이다(Maisonmasse et al., Hydroxychloroquine use against SARS-CoV-2 infection in non-human primates, 2020, Nature 585:584-587). 시험 방법 및 시기: d-28, d0, d7, d14, d21, d28, d35, d47, d49, d50, d51, d54, d62에 혈액학. d-28, d0, d14, d21, d28, d35, d47, d54, d62에 Ab 반응(ELISA, IFA). d-28, d0, d14, d35, d54, d62에 T 세포 반응(ICS, ELISPOT). d47, d49, d50, d51, d54, d62에 사이토카인 반응(LUMINEX). SWABS(바이러스 부하(qRT-PCR-게놈 + 서브게놈): d35, d49, d50, d51, d54, d57, d62에 비강 & 기관 면봉; 기준선 및 d2, d7, d15에 직장 면봉. BAL 바이러스 부하(qRT-PCR-게놈 + 서브게놈): d50. 안락사: 폐 수확, 바이러스 부하(qRT-PCR - 게놈 + 서브게놈): d54, d62. CT 스캔: d35, d50, d57.
도 10. 1/2상 임상 연구: 맹검, 무작위, 용량-증량 연구. 연구 모집단: 18 내지 55세 연령의 150명의 건강한 지원자(용량 군당 50명의 대상체). 투여량: 2회 백신접종(D1, D22; 또한 D0, D21로도 지칭됨), 낮은(~3AU), 중간(~10AU) 및 높은(~40AU) 용량. 면역화 경로: i.m.
도 11. 초기 허가를 위한 비-열등성 중추 3상 면역원성 시험을 위한 연구 설계. 발명의 SARS-CoV-2 백신과 효능이 확립된 허가된 COVID-19 백신 간의 비-열등한 중화 항체 역가를 입증함에 의해 결정된 백신 효능.
도 12. 최종 SARS-CoV-2 제조 공정의 개요―완전히 산업화된 생산 공정.
도 13: 33개의 중화 mAb 또는 각각 클러스터 13, 4, 10, 2, 1, 3의 풋프린트 내 잔기 수. 중화 mAb의 풋프린트 내 및/또는 B.1.1.7, B.1.351 또는 P.1에 대한 돌연변이 위치("x"로 표시)를 정의하는 계통인 잔기가 나열된다. 예를 들어 S-단백질에서 아미노산 위치인 K417 및 E484 돌연변이는 남아프리카 및 브라질 계통에서만 발견된다.
도 14: 실시예 1(iCELLIS 500 생물반응기, 프로타민 설페이트 침전, BPL 불활성화)에 따른 SARS-CoV-2 후보의 2개 샘플의 SDS-PAGE, 은 염색. 밴드는 분명히 3가지 주요 바이러스 단백질(스파이크-단백질, 막-단백질, 핵단백질)뿐만 아니라 숙주 시스템으로부터의 배경 단백질에 기인할 수 있다.
도 15: PHE으로부터 균주 UK MIG457(B.1.1.7 계통) 및 균주 SA_P2(B.1.351 계통)의 스파이크 단백질 내 SARS-CoV-2 돌연변이.
도 16: 사이노몰구스 마카크에서 백신 후보의 면역원성. A) 플레이트를 T4 삼량체화 도메인을 갖는 스파이크 단백질의 엑토도메인으로 코팅하였다. B) 플레이트를 스파이크 당단백질의 수용체 결합 도메인(RBD)으로 코팅하였다. C) 플레이트를 핵단백질로 코팅하였다.
도 17: SNT에 의해 결정된 중화 역가. 점선은 검출의 한계를 나타낸다(LOD, SNT50 = 5). 5 미만의 SNT50 역가를 갖는 샘플은 2.5로 전가되었다.
도 18: RT-qPCR에 의해 결정된 SARS-CoV-2 RNA의 게놈 카피. A) 비인두 면봉. B) 기관 면봉. 점선은 검출의 한계(LOD = 476 RNA 카피/mL) 및 정량의 한계(LOQ = 4760 RNA 카피/mL)를 나타낸다. RNA 카피/mL가 476 미만인 샘플은 238로 전가되었다.
도 19: RT-qPCR에 의해 결정된 SARS-CoV-2 RNA의 서브게놈 카피. A) 비인두 면봉. B) 기관 면봉. 점선은 검출의 한계(LOD = 749 RNA 카피/mL) 및 정량의 한계(LOQ = 7490 RNA 카피/mL)를 나타낸다. RNA 카피/mL가 749 미만인 샘플은 375로 전가되었다.
도 20: RT-qPCR에 의한 기관지폐포 세정액의 분석. A) 게놈 RNA, RNA 카피/mL가 476(LOD) 미만인 샘플은 238로 전가되었다. B) 서브게놈 RNA, RNA 카피/mL가 749(LOD) 미만인 샘플은 375로 전가되었다. 점선은 각 분석에서 검출의 한계(LOD) 및 정량의 한계 (LOQ)를 나타낸다.
도 21: 각 처리 그룹에 대한 수컷 및 암컷 랫트에 대한 GMT의 그래픽 표시.
위약은 연구 과정 동안 정사각형 기호(수컷: 채워진 기호, 암컷: 채워지지 않은 기호)이고 불활성화된 SARS-CoV-2 백신은 원형 기호(수컷: 채워진 기호, 암컷: 채워지지 않은 기호)이다. 오차 막대는 95% 신뢰 구간(CI)을 나타낸다. GMT <50은 25로 전가되었다.
도 22: 결합된 수컷 및 암컷 랫트에 대한 데이터인, 각 처리 그룹에 대한 GMT의 그래픽 표시. 위약은 정사각형 기호이고 불활성화된 SARS-CoV-2 백신은 원형 기호이다. 오차 막대는 95% 신뢰 구간(CI)을 나타낸다. GMT <50은 25로 전가되었다.
도 23: 프로토콜 분석 세트(N=150)당 용량 군에 의한 시간 경과에 따른 SARS-CoV-2 중화 항체(MNA50)의 플롯. 1일차: 낮음(N=51), 중간(N=49) 및 높음(N=50). 8일차: 낮음(N=51), 중간(N=49), 및 높음(N=50). 22일차: 낮음(N=50), 중간(N=48) 및 높음(N=48). 36일차: 낮음(N=51), 중간(N=48), 및 높음(N=50). 그래프는 GMT 및 95% CI를 나타낸다. 산점도는 중화 항체 역가의 실제 분포이다.
도 24: 프로토콜 분석 세트(N=150)당 용량 군에 의한 36일차에 대한 SARS-CoV-2 중화 항체 역가(ND50)에 대한 역누적 분포 함수. 낮은 용량(N=51), 중간 용량(N=48) 및 높은 용량(N=50).
도 25: 중화 역가는 S-단백질 특이적 IgG와 상관관계가 있다. 프로토콜 분석 세트(N=150)당 중화 항체 역가 ND50(MNA)과 IgG 항체 역가(ELISA) 사이의 산점도. 산점도는 ELISA(ELU/mL)와 MNA(ND50)의 결과 간의 상관관계를 나타낸다. ELISA(ELU/mL)와 MNA(ND50) 사이의 피어슨 상관계수(r)와 상관계수의 유의성을 시험하기 위한 P-값이 또한 플롯에 제시되어 있다. 빨간색 점선은 ELISA(50.3ELU/mL) 및 MNA(ND50=58)에 대한 검출의 한계를 나타낸다.
도 26: 프로토콜 분석 세트(N=150)당 용량 군에 의한 시간 경과에 따른 S-단백질 특이적 IgG 항체 역가(ELISA)의 플롯. 1일차: 낮음(N=51), 중간(N=49) 및 높음(N=50). 8일차: 낮음(N=51), 중간(N=49), 및 높음(N=50). 22일차: 낮음(N=51), 중간(N=49) 및 높음(N=50). 36일차: 낮음(N=51), 중간(N=49), 및 높음(N=50). 그래프는 GMT 및 95% CI를 나타낸다. 산점도는 IgG 항체 역가의 실제 분포이다.
도 27: 패널 14 스파이크 단백질, 전체 서열(N=150)에 대한 용량 군 및 평가 날짜에 의한 2x10^5 PBMC당 IFN 감마 반점 형성 단위의 플롯. 용량 군 비교(p값): 1일차(p = 0.321) 및 36일차(p<0.001). 1일차: 낮음(N=46), 중간(N=43) 및 높음(N=44). 36일차: 낮음(N=44), 중간(N=44) 및 높음(N=45). 박스플롯은 중간, 하위 사분위수 및 상위 사분위수를 나타낸다. 각 막대 내의 수평선은 중간 값이고 더하기 기호(+)는 각 그룹에 대한 평균 값을 나타낸다. 산점도는 각 그룹 내 2.5x10^5 PBMC당 SFU의 실제 분포이다.
도 28: 막 단백질(N=150)에 대한 용량 군 및 평가 날짜에 의한 2x10^5 PBMC당 IFN 감마 반점 형성 단위의 플롯. 용량 군 비교(p값): 1일차(p=0.704), 36일차(p-0.014). 1일차: 낮음(N=46), 중간(N=43) 및 높음(N=44). 36일차: 낮음(N=44), 중간(N=44) 및 높음(N=45). 박스플롯은 중간, 하위 사분위수 및 상위 사분위수를 나타낸다; 각 막대 내의 수평선은 중간 값이고 더하기(+) 기호는 각 그룹에 대한 평균 값을 나타낸다. 산점도는 각 그룹 내 2.5x10^5 PBMC당 SFU의 실제 분포이다.
도 29: 뉴클레오캡시드(N=150)에 대한 용량 군 및 평가일에 의한 2x10^5 PBMC당 IFN 감마 반점 형성 단위의 플롯. 용량 군 비교(p값): 1일차(p=0.378), 36일차(p=0.008). 1일차: 낮음(N=46), 중간(N=43) 및 높음(N=44). 36일차: 낮음(N=44), 중간(N=44) 및 높음(N=45). 박스플롯은 중간, 하위 사분위수 및 상위 사분위수를 나타낸다. 각 막대 내의 수평선은 중간 값이고 더하기(+) 기호는 각 그룹에 대한 평균 값을 나타낸다. 산점도는 각 그룹 내 2.5x10^5 PBMC당 SFU의 실제 분포이다.
본 발명의 실시형태는 CpG 어쥬번트와 조합한 불활성화된 SARS-CoV-2 입자를 포함하는 SARS-CoV-2 백신 또는 면역원성 조성물에 관한 것이다. 전형적으로 불활성화된 SARS-CoV-2 입자는 전체 불활성화된 입자이며, 즉 불활성화된 바이러스 입자는 불활성화되어진 전체 천연 SARS-CoV-2 입자로부터 유래된다. 본 명세서에 사용된 "SARS-CoV-2"는 SARS-CoV-2 바이러스를 지칭하고 "SARS-CoV-2 입자"는 전형적으로 전체 SARS-CoV-2 바이러스 입자, 즉 비리온을 지칭한다.
일 실시형태에서, 불활성화된 SARS-CoV-2 입자는 Th1 반응-지향 어쥬번트 CpG, 바람직하게는 CpG 1018과 조합된다. 본 명세서에 사용된 "CpG"는, 예를 들어, 톨-유사 수용체 9(TLR9) 작용제로 작용할 수 있는, 시토신-포스포-구아노신(CpG) 모티프-함유 올리고데옥시뉴클레오티드(또는 CpG-ODN)를 지칭한다. CpG 모티프는, 예를 들어, TLR9에 결합할 수 있는, 메틸화되지 않은 시티딘-포스포-구아노신 디뉴클레오티드 서열을 지칭한다. CpG와 같은 Th1 반응-지향 어쥬번트는 면역화된 대상에서 (Th2 유형 반응보다) 우세한 T 헬퍼 유형 1(즉, Th1) 면역 반응, 즉 "Th1-편향된 반응"의 유도를 촉진한다. 일반적으로 사용되는 백신의 Th1- 또는 Th2-지향 특성은 당업계에 공지되어 있다. 놀랍게도 Th1 반응을 촉진하는 어쥬번트, 예를 들어 CpG 1018을 사용하면 백신의 면역원성과 따라서 항바이러스 반응을 개선할 수 있을 뿐만 아니라 면역병리(가능하기로는 바이러스 성분에 대한 과민증으로 인한 주로 Th2 유형 반응으로부터 유래할 수 있음)와 같은 불리한 효과의 위험을 감소시킬 수 있다는 것이 밝혀졌다. 일 실시형태에서, 본 발명의 SARS-CoV-2 백신은 또한 Th2-자극 어쥬번트 명반을 포함하며, 이는 인간에서 Th2 편향에 대해 알려진 경향을 갖는다. 중화 항체로서, 그 생산이 항-바이러스성 면역에 중요한 이것은 Th2-자극 어쥬번트, 예컨대 예를 들어 명반에 의해 강력하게 자극된다. 항-바이러스성 면역에 중요한 것은 또한 세포 면역 반응이며, 이는 명반에 의해서 단지 약하게 자극된다. 함께 전달되는, 명반과 Th1-유도 어쥬번트, 예컨대 CpG는 강력한 항-바이러스성 반응을 제공할 수 있다. 요컨대, 백신 제형에서 CpG와 명반을 함께 사용하면 체액 및 세포 성분 둘 모두를 포함하는 항원에 대해 보다 균형잡힌 면역 반응을 제공할 수 있고 주로 Th2 반응으로 명반 단독보다 덜 해로운 영향을 미칠 수 있다.
일 실시형태에서, 발명의 백신에 포함된 CpG 어쥬번트는 클래스 A, 클래스 B 또는 클래스 C CpG(표 A-1 참조), 바람직하게는 클래스 B CpG이다. 클래스 B CpG 분자는 CpG 1018, CpG 1826 및 CpG 7909를 포함한다(각각 서열번호: 4, 7 및 8; 표 A-2). CpG 1018이 가장 바람직하다.
표 A-1. CpG 클래스 A, B 및 C의 비교 특성(Campbell JD, 2017, in Christopher B. Fox (ed.), Vaccine Adjuvants: Methods and Protocols, Methods in Molecular Biology, vol. 1494, DOI 10.1007/978-1-4939-6445-1_2).
Figure pct00001
IFN, 인터페론; pDC, 형질세포양 수지상 세포
표 A-2. 클래스 B CpG
Figure pct00002
CpG 1018은 명반 상에 흡착될 수 있고 따라서 Th1 및 Th2 반응 둘 모두를 유도하는 조합 어쥬번트로 사용될 수 있다(예를 들어 Tian et al. Oncotarget, 2017, Vol. 8, (No. 28), pp: 45951-45964에 기술된 바와 같음). 따라서, 일 실시형태에서, 발명의 CpG-어쥬번트된 백신 조성물은 알루미늄(Al3+), 바람직하게는 알루미늄 염의 형태, 예를 들어, 산화알루미늄, 수산화알루미늄 또는 인산알루미늄, 바람직하게는 수산화알루미늄을 추가로 포함한다. 바람직한 알루미늄 염은, 예를 들어, WO2013/083726 또는 Schlegl et al., Vaccine 33 (2015) 5989-5996에 상세히 기재된 어쥬번트인, 백신 조성물의 중량을 기준으로 1.25ppb 미만으로, Cu 함량이 감소된 수산화알루미늄이다. 바람직한 실시형태에서, 백신 조성물은 CpG 및 Al3+ 둘 모두를 포함하며, 즉, 어쥬번트는 CpG 및 Al3+, 바람직하게는 CpG 1018(서열번호: 4) 및 수산화알루미늄(Al(OH)3)의 형태로 제공된 Al3+의 조합 또는 혼합물이다. Al3+의 존재는 CpG의 필요한 용량을 감소시킬 수 있으며, 즉 "용량-절약" 효과가 있다. 일 실시형태에서, SARS-CoV-2 백신은 Al3+와 함께 제형화되고, 대상체의 백신접종 직전에 별도의 CpG-함유 용액과 조합되며; 즉, "베드-사이드 혼합"된다. 바람직한 실시형태에서, 2개의 어쥬번트는 발명의 SARS-CoV-2 백신의 제형에 둘 모두 포함되며; 즉, "단일-바이알 제형"이다.
일 실시형태에서, 백신 조성물에서 Al3+:CpG 중량/중량(w/w) 비율, 바람직하게는 Al(OH)3의 형태로 제공되는 Al3+:CpG 1018(w/w) 비율은 인간에서 약 1:10, 약 1:5, 약 1:4, 약 1:3, 약 1:2, 약 1:1, 약 2:1, 약 3:1, 약 4:1, 약 5:1, 약 10:1, 바람직하게는 약 1:3 내지 3:1, 보다 바람직하게는 약 1:2 내지 1:1, 가장 바람직하게는 약 1:2, 더욱 더 바람직하게는 1:2이다. 이와 관련하여 마우스와 인간에서 CpG의 효과는 부분적으로 CpG-DNA 서열에 다르게 반응하는 서로 다른 TLR9 수용체뿐만 아니라 마우스와 인간에서 TLR9 발현의 독특한 세포 분포 패턴으로 인해 매우 다른 것으로 알려져 있다(Chuang TS et al. 2002 Toll-like receptor 9 mediates CpG-DNA signaling. J Leukocyte Biol. (71)538-544). 따라서 주어진 항원에 대한 최적의 반응을 제공하는 명반:CpG 비율은 인간 및 마우스에서 아마도 실질적으로 다를 수 있다. 본 명세서에서 언급된 명반 성분의 중량은 사용된 알루미늄염의 유형에 관계없이 용액 내 Al3+의 중량을 지칭한다. 예를 들어, 0.5mg의 Al3+는 1.5mg 명반에 해당한다. 일 실시형태에서, SARS-CoV-2 백신 조성물에 존재하는 명반(Al3+)의 양은 약 0.1 내지 2mg/mL, 약 0.2 내지 1.5mg/mL, 약 0.5 내지 1.3mg/mL, 특히 약 0.8 내지 1.2mg/mL, 가장 바람직하게는 약 1mg/mL, 즉, 0.5mg/용량이다. 바람직한 실시형태에서, 비교적 높은 양의 명반(현재 허가된 명반-어쥬번트된 백신과 비교됨)은 항원의 완전한 결합뿐만 아니라 제형 내 총 CpG의 적어도 일부의 결합을 보장하기 위해 사용된다. 이와 관련하여, 명반:CpG의 비율은 "유리" 탈착된 CpG, 즉 백신 조성물에서 명반 및/또는 항원에 결합되지 않은 CpG의 양에 영향을 미친다. 바람직한 실시형태에서, 백신 조성물 중 유리(비결합) CpG의 양은 예를 들어 중량에 의해(백신 조성물 내 CpG의 총 중량을 기준으로) 10% 초과, 20% 초과, 30% 초과, 40% 초과, 50% 초과, 60% 초과, 70% 초과, 80% 초과, 90% 초과, 95% 초과, 바람직하게는 약 70% 내지 95%, 가장 바람직하게는 약 80% 내지 90%이다. 특히, 명반:CpG 비율은 대부분의 CpG 함량이 "유리"(탈착) CpG로 용이하게 해야 하며; 즉, CpG는 저장소에 남아 있도록 백신의 성분에 결합되지 않는다. 바람직한 실시형태에서, 유리 CpG 대 결합 CpG의 양은, 예를 들어 중량에 의해(즉, 백신 조성물 내 CpG의 총 중량을 기준으로 한 중량에 의한 유리 CpG의 양), 50% 초과, 60% 초과, 70% 초과, 80% 초과, 85% 초과, 90% 초과, 바람직하게는 70 초과 내지 90%, 특히 약 80 내지 90%이다. 일 실시형태에서, 본 발명의 SARS-CoV-2 백신 조성물 내 CpG의 양은 약 0.25 내지 6mg/mL, 약 0.5 내지 3mg/mL, 약 1 내지 3mg/mL, 특히 약 1.5 내지 2.5mg/mL, 가장 바람직하게는 약 2mg/mL, 즉, 1mg/용량이다. 특히 바람직한 실시형태에서, 본 발명의 SARS-CoV-2 백신 조성물은 1mg/mL Al3+ 및 2mg/mL CpG 1018; 즉, 용량당 0.5mg Al3+ 및 1mg/mL CpG 1018로 어쥬번트된다.
CpG에 대한 명반의 비율에 부가하여, 다양한 완충액 시스템이 백신 조성물에서 Al3+ 및/또는 항원에 대한 CpG의 흡착에 영향을 미칠 수 있다. 예를 들어, 백신 조성물에서 Tris 완충액 시스템을 사용하면 감소된 유리 CpG(즉, 감소된 탈착)를 초래하는 반면, 포스페이트 완충액 시스템은 CpG의 더 나은 탈착을 허용한다. 특정 예에서, 1mg/mL Al3+(수산화알루미늄) 및 2mg/mL CpG 1018(1:2 w/w 비율)을 함유하는 포스페이트-완충 제형은 단지 약 10-20% 결합된 CpG, 즉 약 80-90% 유리 CpG를 갖는다. 인산염 완충액에 제형화할 때 약 0.3mg의 CpG가 Al3+의 mg당 흡착된다(데이터는 표시되지 않음).
전형적으로 어쥬번트는 백신 제품의 제조 동안 불활성화된 SARS-CoV-2 입자와 조합되며, 즉 제조된 백신 제품은 어쥬번트를 포함하고 이 형태로 판매/유통된다. 대안적 실시형태에서 어쥬번트는 사용의 시점, 예를 들어, 백신의 임상 투여 직전(때때로 백신 성분의 "베드사이드 혼합"으로도 지칭됨) 불활성화된 SARS-CoV-2 입자와 조합될 수 있다. 따라서, 본 발명은 불활성화된 SARS-CoV-2 입자 및 본 명세서에 기재된 어쥬번트를 포함하는 양 백신 제품, 뿐만 아니라 이의 개별 성분(예를 들어 베드사이드 혼합에 적합함)을 포함하는 키트, 및 SARS-CoV-2 감염을 예방하거나 치료하는데 백신의 개별 성분의 조합된 사용을 포함한다.
본 발명의 일부 실시형태에서, SARS-CoV-2 입자는 그의 표면 구조를 실질적으로 변형시키지 않으면서 불활성화된다. 달리 말하면, SARS-CoV-2 입자의 천연 표면 형태는 불활성화된 바이러스 입자에 유지된다. 예를 들어, 베타-프로피오락톤을 사용하여, 불활성화 과정을 최적화함에 의해, 천연 SARS-CoV-2 입자의 감염성은 그의 항원성 및/또는 면역원성에 부정적인 영향을 미치지 않으면서 실질적으로 폐기, 즉 완전하게 폐지될 수 있다는 것이 놀랍게도 발견되었다. 따라서, 본 발명은 일 양태에서 SARS-CoV-2 감염에 대한 중화 항체 및/또는 보호 면역을 생성하는 불활성화된 바이러스 백신(예를 들어, 베타-프로피오락톤-불활성화된 바이러스 백신)을 제공한다.
일 실시형태에서, SARS-CoV-2 입자는 바이러스 RNA를 우선적으로 표적화하는 방법에 의해 불활성화된다. 이것은 예를 들어 불활성화 단계는 바이러스 단백질보다 바이러스 RNA를 더 많이 변형한다는 것을 의미한다. 따라서, 불활성화된 SARS-CoV-2 입자는 복제-결함 바이러스 RNA를 포함할 수 있으며, 즉 바이러스 RNA는 불활성화된 입자가 복제할 수 없도록 불활성화 단계에서 변형된다. 바이러스 RNA를 우선적으로 표적화하는 불활성화 방법을 이용함에 의해, 본 발명은 유리하게는 바이러스 표면 단백질에서 면역원성 에피토프의 보존을 허용한다.
바람직하게는, 불활성화 방법은 바이러스 RNA에 비해 바이러스(표면) 단백질을 보존하며, 예를 들어 바이러스 표면 단백질(예를 들어 스파이크(S) 단백질)은 바이러스 RNA와 비교하여 불활성화 단계로 인한 변형을 적거나 보다 드물게 포함할 수 있다. 예로써, 바이러스 표면 단백질(예를 들어 S 단백질)에서 아미노산 잔기의 더 낮은 비율은 바이러스 RNA에서 변형된 뉴클레오티드 잔기의 비율과 비교하여 불활성화 단계에 의해 변형될 수 있다. 일부 실시형태에서, 바이러스 표면 단백질(예를 들어 S 단백질)에서 변형된 아미노산 잔기의 비율은 바이러스 RNA에서 변형된 뉴클레오티드 잔기의 비율보다 적어도 5%, 10%, 20%, 30%, 50%, 70% 또는 90% 낮을 수 있다. "변형" 또는 "변형된 잔기"는 천연 SARS-CoV-2 입자에 존재하지 않는 비-천연 잔기, 예를 들어 불활성화 단계로 인한 이러한 잔기의 화학적(공유) 변형을 지칭하는 것으로 의미된다.
일 실시형태에서, 바이러스 RNA는 알킬화 및/또는 아실화에 의해 불활성화되며, 즉 SARS-CoV-2 불활성화된 입자에서 변형은 알킬화 및/또는 아실화된 뉴클레오티드 잔기를 포함한다. 일부 실시형태에서, 변형은 퓨린(특히 구아닌) 잔기에 우선적으로 표적화되며, 예를 들어, SARS-CoV-2 불활성화된 입자는 하나 이상의 변형된 (예를 들어 알킬화 또는 아실화된) 구아닌 잔기를 포함한다. 일부 경우에, 불활성화 단계는, 예를 들어 바이러스 RNA에서 구아닌 잔기를 통해 바이러스 RNA와 바이러스 단백질의 교차-결합을 초래할 수 있다. 불활성화 단계는 또한, 예를 들어 바이러스 게놈의 단편화를 초래하는, 바이러스 RNA에 닉 또는 가닥 절단을 도입할 수 있다.
적합한 알킬화제 및/또는 아실화제는 당업계에 공지되어 있다. 일 실시형태에서, 불활성화제는 베타-프로피오락톤을 포함하며, 즉 백신은 베타-프로피오락톤-불활성화된 바이러스 입자를 포함한다. 임의의 경우에, 특정 실시형태에서, 베타-프로피오락톤(본 명세서에서 "BPL"로도 지칭됨) 처리는 실질적으로 불활성이지만, 천연 SARS-CoV-2에 존재하는 중화 에피토프에 대해 높은 항원성과 면역원성을 유지하는 SARS-CoV-2 입자를 초래하기 때문에 본 발명에 따라 특히 바람직하다. 특히, 베타-프로피오락톤이 최소 수의 단백질 변형으로 SARS-CoV-2 입자를 불활성화하는데 사용될 수 있다는 것이 놀랍게도 발견되었다. 예로써, 하기 실시예 7 및 10에서 입증된 바와 같이, 베타-프로피오락톤을 사용한 SARS-CoV-2 입자의 불활성화는 베타-프로피오락톤에 의한 인플루엔자 입자의 불활성화와 비교하여 훨씬 더 적은 수의 바이러스 단백질의 변형을 초래한다. 따라서 베타-프로피오락톤-불활성화된 SARS-CoV-2 입자에서 바이러스 입자의 천연 표면 형태가 보존될 수 있다.
발명의 바람직한 실시형태에서, 바이러스 RNA는 최적화된 방식으로 불활성화되는데, 즉 그것은 더 이상 감염성이 아닐 정도로 충분하게 불활성화되지만 "과도하게"-불활성화되지 않아 특히 S-단백질에서 상이한 아미노산에서의 수많은 변형이 일어난다. 더욱 더 보다 바람직한 실시형태에서, BPL 불활성화는 SARS-CoV-2 바이러스를 충분하게 불활성화시킬 뿐만 아니라(그러나 과도하게-불활성화시키지는 않음) 제조 공정에서 공동-농후화되고 공동-배양될 수 있는 바이러스를 충분하게 불활성화시킨다(예를 들어 실험 부분 참조). 공동-배양 및 공동-농후화될 수 있는 불활성화하려는 특정 하드 바이러스는 PPV(돼지 파보바이러스)이다. 실험 부분 참조. 불활성화 단계에서 베타-프로피오락톤의 농도는 바이러스에서 표면 단백질의 형태를 보존하면서 바이러스 복제의 완전한 억제를 보장하도록 최적화될 수 있다. 예를 들어, 불활성화 단계에서 베타-프로피오락톤의 농도는 예를 들어 0.01 내지 1 중량%, 바람직하게는 0.01 내지 0.1 중량%, 더욱 바람직하게는 약 0.03 중량%일 수 있다. BPL의 바람직한 양은 SARS-CoV-2 바이러스 뿐만 아니라 다른 관련 바이러스/불순물이 불활성화되는 반면 S-단백질의 아미노산 대부분이 보존되는 경우(즉, 변형되지 않음) 500ppm인것으로 밝혀졌다(즉, 적은 수의 아미노산만이 낮은 확률로 변형되는 것으로 나타났다).
일부 실시형태에서, 천연 SARS-CoV-2 입자는 적어도 5시간, 적어도 10시간, 적어도 24시간 또는 적어도 4일, 예를 들어 5 내지 24시간 또는 그 이상 예컨대 48시간 동안 베타-프로피오락톤과 접촉될 수 있다. 불활성화 단계는 약 0℃ 내지 약 25℃, 바람직하게는 약 4℃ 또는 약 22℃, 또는 예를 들어 약 18 내지 24℃에서 수행될 수 있다. 일 실시형태에서, 불활성화 단계(예를 들어, 베타-프로피오락톤 사용)는 2℃ 내지 8℃에서 24시간 동안 수행된다. 불활성화 단계는 선택적으로 그리고 바람직하게는 당업계에 공지된 바와 같이 불활성화제의 가수분해 단계가 뒤따를 수 있다(이는 베타-프로피오락톤에 대해 예를 들어 약 37℃+/- 2℃에서 2.5시간 +/- 0.5시간의 총 시간 동안 수행될 수 있다). 전형적으로, 불활성화 단계에서 더 긴 인큐베이션 시간 및/또는 더 높은 온도는 바이러스 불활성화를 향상시킬 수 있지만, 또한 바이러스 입자의 바람직하지 않은 표면 변형의 증가된 위험을 초래하여 면역원성을 감소시킬 수 있다. 따라서, 불활성화 단계는 예를 들어 완전히 불활성화된 바이러스 입자를 생성하기 위해 필요한 최단 시간 동안 수행될 수 있다. 가수분해의 완료 후, 불활성화된 바이러스 용액은 일 실시형태에서 즉시 5±3℃로 냉각되었고 불활성화가 대용량 플라크 검정 및 일련의 계대 검정에 의해 확인될 때까지 거기에 보관되었다.
SARS-CoV-2 입자의 베타-프로피오락톤 불활성화는 시스테인, 메티오닌 및/또는 히스티딘 잔기를 우선적으로 변형할 수 있다. 따라서 일부 실시형태에서, 불활성화된 SARS-CoV-2 입자는 하나 이상의 베타-프로피오락톤-변형된 시스테인, 메티오닌 및/또는 히스티딘 잔기를 포함한다. 그러나, 본 발명의 실시형태에서, 베타-프로피오락톤-불활성화된 SARS-CoV-2 입자는 비교적 적은 단백질 변형을 나타낸다. 따라서, 예를 들어, 백신에서 불활성화된 SARS-CoV-2 입자는 200, 100, 50, 30, 20, 15, 10, 9, 8, 7 또는 6개 미만의 베타-프로피오락톤-변형된 아미노산 잔기를 포함할 수 있다. 바람직하게는 불활성화된 SARS-CoV-2 입자의 스파이크(S) 단백질은 100, 50, 30, 20, 15, 10, 9, 8, 7 또는 6개 미만의 베타-프로피오락톤-변형된 아미노산 잔기를 포함한다. 더욱 바람직하게는 불활성화된 SARS-CoV-2 입자 또는 이의 스파이크 단백질은 20개 이하, 15개 이하, 10개 이하, 또는 5개 이하의 베타-프로피오락톤-변형된 아미노산 잔기를 포함한다. 가장 바람직하게는 불활성화된 SARS-CoV-2 입자 또는 이의 스파이크 단백질은 1 내지 100, 2 내지 70, 3 내지 50, 4 내지 30, 5 내지 25, 5 내지 20, 10 내지 20 또는 약 15개의 베타-프로피오락톤-변형된 아미노산 잔기를 포함한다.
또 다른 실시형태에서, SARS-CoV-2 폴리펩티드의 20%, 15%, 10%, 5% 또는 4% 미만이 베타-프로피오락톤-변형된다. 예로써, 입자 내 SARS-CoV-2 폴리펩티드의 0.1 내지 10%, 1 내지 8%, 2 내지 7% 또는 약 3%, 4%, 5% 또는 6%가 베타-프로피오락톤-변형될 수 있다. 백신에서 잔기 및/또는 폴리펩티드의 베타-프로피오락톤 변형은 질량 분석법, 예를 들어, 예로써 실시예 7 또는 실시예 10에 기술된 방법을 사용하여 탠덤 질량 분석법(LC-MS-MS)과 함께 액체 크로마토그래피를 사용함에 의해 검출될 수 있다. 이러한 방법에서, SARS-CoV-2 입자는 LC-MS-MS 분석을 위해 단백질을 SARS-CoV-2 폴리펩티드로 단편화하기 위해 단리될 수 있다. 단리 단계는 임의의 적합한 효소 또는 효소의 조합에 의해, 예를 들어, 트립신, 키모트립신 및/또는 PNGase F(펩티드:N-글리코시다제 F)에 의해, 또는 예를 들어 산 가수분해에 의해 수행될 수 있다. 바람직하게는 효소 분해 또는 산 가수분해에 이은 LC-MS-MS에 의해 검출된 BPL-변형된 폴리펩티드의 백분율은 다음과 같다: (a) 트립신 단리, 1 내지 5%, 2 내지 4% 또는 약 3%; (b) 트립신 + PNGase F 단리, 1 내지 5%, 2 내지 4% 또는 약 3%; (c) 키모트립신, 1 내지 10%, 3 내지 8% 또는 약 6%; (d) 산 가수분해, 1 내지 6%, 2 내지 5% 또는 약 4%. 이 맥락에서, "베타-프로피오락톤-변형된" 폴리펩티드는 폴리펩티드가 적어도 하나의 베타-프로피오락톤 변형, 예를 들어 적어도 하나의 베타-프로피오락톤-변형된 잔기를 포함한다는 것을 의미한다.
일부 실시형태에서, 불활성화된 SARS-CoV-2 입자의 스파이크(S) 단백질은 다음의 잔기 중 하나 이상에서 베타-프로피오락톤 변형을 포함한다: 예를 들어, 서열번호: 3에서 49, 146, 166, 177, 207, 245, 379, 432, 519, 625, 1029, 1032, 1058, 1083, 1088, 1101, 1159 및/또는 1271, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치. 바람직하게는 불활성화된 SARS-CoV-2 입자는 다음의 잔기 중 하나 이상에서 베타-프로피오락톤 변형을 포함한다: 예를 들어, 서열번호: 3에서 H49, H146, C166, M177, H207, H245, C432, H519, H625, M1029, H1058, H1083, H1088, H1101, H1159 및/또는 H1271, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치. 다른 실시형태에서, 불활성화된 SARS-CoV-2 입자는 다음의 잔기 중 하나 이상에서 베타-프로피오락톤 변형을 포함한다: 예를 들어, 서열번호: 3에서 H207, H245, C379, M1029 및/또는 C1032, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치. "상응하는 위치"는 예를 들어 서열번호: 19, 21, 23, 25 또는 27을 NCBI Basic Local Alignment Search Tool(BLAST)과 같은 프로그램을 사용하여 서열번호: 3과 정렬될 때 서열번호: 3에서 위치 H207, H245, C379, M1029 및/또는 C1032와 정렬하는 서열번호: 19, 21, 23, 25 또는 27에서의 위치를 의미한다.
예로써, 일부 실시형태에서, 서열번호: 3에서 H207, H245, C379, M1029 및 C1032에 상응하는 서열번호: 19, 21, 23, 25 또는 27에서의 위치가 하기에 나타나 있다:
Figure pct00003
일부 실시형태에서, 불활성화된 SARS-CoV-2 입자의 막(M) 당단백질은 다음의 잔기 중 하나 이상에서 베타-프로피오락톤 변형을 포함한다: 예를 들어, 서열번호: 29에서 125, 154, 155, 159 및/또는 210, 바람직하게는 H154, H155 , C159 및/또는 H210.
일부 실시형태에서, 불활성화된 SARS-CoV-2 입자의 뉴클레오캡시드(N) 단백질은, 예를 들어 서열번호: 28에서의 M234에서 베타-프로피오락톤 변형을 포함한다.
일부 실시형태에서, 불활성화된 SARS-CoV-2 입자에서 다음의 잔기 중 하나 이상의 30%, 20%, 10%, 5%, 3% 또는 1% 미만은 베타-프로피오락톤 변형된다: (i) 스파이크(S) 단백질에서, 예를 들어, 서열번호: 3, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치에서: 잔기 49, 146, 166, 177, 207, 245, 379, 432, 519, 625, 1029, 1032, 1058, 1083, 1088, 1101, 1159 및/또는 1271; 바람직하게는 H49, H146, C166, M177, H207, H245, C432, H519, H625, M1029, H1058, H1083, H1088, H1101, H1159 및/또는 H1271; 대안적으로 H207, H245, C379, M1029 및/또는 C1032; (ii) 막(M) 당단백질에서, 예를 들어, 서열번호: 29에서: 잔기 125, 154, 155, 159 및/또는 210; 바람직하게는 H154, H155, C159 및/또는 H210; 및/또는 (iii) 예를 들어, 서열번호: 29에서 뉴클레오캡시드(N) 단백질의 M234. 바람직한 실시형태에서, 불활성화된 SARS-CoV-2 입자에서 상기 잔기의 적어도 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 또는 각각의 30%, 20%, 10%, 5%, 3% 또는 1% 미만은 베타-프로피오락톤 변형된다. 이 단락에서, 변형된 잔기의 백분율은, 예를 들어, 하기 실시예 7 또는 10에 기술된 바와 같이 단백질 풍부도로 정규화된 동일한 변형 부위에 대한 변형된 펩티드 대 비변형된 펩티드의 %인, 부위 점유를 지칭하기 위한 것이다.
또 다른 바람직한 실시형태에서, 불활성화된 SARS-CoV-2 입자에서의 다음 위치에서 베타-프로피오락톤-변형된 잔기(즉, 부위 점유)의 비율은 다음과 같다:
(i) 스파이크(S) 단백질(예를 들어, 서열번호: 3, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치)에서:
(a) H207: 30% 미만, 바람직하게는 0.01 내지 25%; 및/또는
(b) H245: 10% 미만, 바람직하게는 0.1 내지 5%; 및/또는
(c) C379: 5% 미만, 1% 미만 또는 0.1% 미만; 및/또는
(d) M1029: 5% 미만, 1% 미만 또는 0.1% 미만; 및/또는
(e) C1032: 5% 미만, 1% 미만 또는 0.1% 미만; 및/또는
(ii) 막(M) 당단백질(예를 들어, 서열번호: 29)에서:
(f) H154: 5% 미만, 1% 미만 또는 0.1% 미만; 및/또는
(g) H155: 10% 미만, 바람직하게는 0.1 내지 5%; 및/또는
(h) C159: 5% 미만, 1% 미만 또는 0.1% 미만; 및/또는
(i) H210: 20% 미만, 바람직하게는 0.1 내지 10%; 및/또는
(iii) 뉴클레오캡시드(N) 단백질(예를 들어, 서열번호 28)에서:
(j) M234: 90% 미만, 10% 미만 또는 0.1% 미만.
또 다른 바람직한 실시형태에서, 불활성화된 SARS-CoV-2 입자의 스파이크(S) 단백질에서 다음 위치 각각(예를 들어, 서열번호: 3, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치)에서 베타-프로피오락톤-변형된 잔기(즉, 부위 점유)의 비율은 다음과 같다:
(a) 잔기 H49, H146, C166, H207, H519, M1029, H1083, H1088, H1101, H1159 및/또는 H1271: 20% 미만, 바람직하게는 0.01 내지 10%, 더욱 바람직하게는 0.1 내지 5%; 및/또는
(b) 잔기 M177, C432, H625: 30% 미만, 바람직하게는 0.1 내지 20%, 더욱 바람직하게는 1 내지 10%; 및/또는
(c) 잔기 H245, H1058: 30% 미만, 바람직하게는 0.1 내지 20%, 더욱 바람직하게는 5 내지 15%;
일부 실시형태에서, 불활성화된 SARS-CoV-2 입자(또는 그의 스파이크(S) 단백질)에서 베타-프로피오락톤-변형된 아미노산 잔기의 비율은 베타-프로피오락톤-불활성화된 인플루엔자 입자(또는 이의 헤마글루티닌(HA) 또는 뉴라미니다제(NA) 단백질)에서, 예를 들어 SARS-CoV-2 입자에 유사한 조건 하에서 불활성화된 인플루엔자 입자에서 변형된 잔기의 비율보다 적어도 5%, 10%, 20%, 30%, 50%, 70% 또는 90% 더 낮을 수 있다.
대안적인 실시형태에서, 바이러스 RNA는 자외선(UV) 광으로 처리에 의해 불활성화될 수 있다. UV 처리는 바이러스 입자에서 (폴리펩티드와 비교하여) RNA를 우선적으로 표적화하는 데 사용될 수 있어, 예를 들어, 변형된 뉴클레오티드 및/또는 단편화를 초래한다. 일부 실시형태에서, UV 처리는 바이러스의 불활성화를 개선하기 위해 베타-프로피오락톤 처리와 조합될 수 있으며, 예를 들어 베타-프로피오락톤 처리 단계는 UV 처리 단계가 뒤따르거나 그 반대일 수 있거나, 또는 UV 처리 단계는 베타-프로피오락톤 처리 단계와 동시에 수행될 수 있다.
다른 실시형태에서, 천연 SARS-CoV-2 입자는 포름알데히드를 사용하여 불활성화될 수 있다. 그러나, 포름알데히드 불활성화는 바이러스 표면 단백질에서 면역원성 에피토프를 보존하면서 바이러스 RNA를 우선적으로 표적화하기에 덜 적합하기 때문에 전형적으로 본 발명에서 덜 바람직하다.
따라서 바람직한 실시형태에서, 불활성화 단계(들)(특히 포름알데히드를 사용할 때 뿐만 아니라 예를 들어 베타-프로피오락톤과 같은 다른 불활성화제를 사용할 때)는 표면 항원 완전성, 특히 S 단백질의 완전성을 보존하기 위해 온화한 조건 하에서 수행된다.
일 실시형태에서, 이러한 약한 불활성화 방법은 천연 SARS-CoV-2 입자를 포함하는 액체 조성물을 화학적 바이러스 불활성화제(예컨대 예를 들어, 상기에 열거된 임의의 화학적 불활성화제 또는 그의 조합, 예로써 포름알데히드 또는 바람직하게는 베타-프로피오락톤)와 용기에서 접촉시키고, 난류 흐름이 아닌 층류 흐름의 조건 하에 화학적 바이러스 불활성화제 및 SARS-CoV-2 입자를 포함하는 액체 조성물을 혼합하고, 화학적 바이러스 불활성화제 및 SARS-CoV-2 입자를 포함하는 액체 조성물을 바이러스 입자를 불활성화하기에 충분한 시간 동안 인큐베이션하는 것을 포함한다. 약한 불활성화 단계는 선택적으로 유연한 생물반응기 백에서 수행된다. 약한 불활성화 단계는 바람직하게는 불활성화의 기간 동안 5회 이하의 용기 반전을 포함한다. 바람직하게는, 화학적 바이러스 불활성화제와 천연 SARS-CoV-2 입자를 포함하는 조성물의 혼합은 인큐베이션의 기간 동안 10rpm 이하에서 10분 이하 동안 용기를 흔들기, 회전, 궤도 진탕 또는 진동시키는 것을 포함한다.
적합한 약한 또는 부드러운 불활성화 방법은 하기 실시예에 기재되어 있다. 그러한 방법의 추가 세부사항은 또한 WO 2021/048221에 기술되어 있으며, 그 내용은 그 전체가 본 명세서에 포함된다.
전형적으로, 불활성화 단계는 불활성화된 SARS-CoV-2 입자에 의한 포유동물(예를 들어, 인간) 세포의 감염성을 실질적으로 제거한다. 예로써, 포유동물 세포의 감염성은 천연 SARS-CoV-2 입자와 비교하여 적어도 99%, 99.99% 또는 99.9999% 감소될 수 있거나, 불활성화된 SARS-CoV-2 입자에 의한 인간 세포의 감염성은 검출가능하지 않을 수 없다. 표준 검정, 예를 들어 플라크 검정, TCID50(50% 조직 배양 감염성 용량)의 결정은 잔류 감염성 및 효과적인 바이러스 역가를 결정하는 데 사용할 수 있다. 예로써, 포유동물 세포는 MDCK, COS 또는 Vero 세포일 수 있다.
본 발명의 바람직한 실시형태에서, SARS-CoV-2 입자의 천연 표면 형태는 불활성화된 바이러스 입자에서 보존된다. 이것은 예를 들어 하나 이상의 또는 모든 면역원성(중화) 에피토프는 불활성화된 바이러스 입자에 보유되어, 불활성화된 입자는 인간 대상체에게 투여될 때 천연 SARS-CoV-2 입자에 대한 중화 항체를 생성할 수 있다는 것을 의미한다. "천연 표면 형태"는 천연 SARS-CoV-2 입자, 즉 불활성화되지 않은 SARS-CoV-2 입자(비리온)에서 발견되는 표면 형태를 지칭하는 것으로 의미한다. 대상체에서 중화 항체를 생성할 때 백신 또는 불활성화된 SARS-CoV-2 입자의 특성은 예를 들어 플라크 환원 중화 시험(PRNT 검정), 예를 들어 당해 분야에 공지된 바와 같이 면역화된 대상체로부터의 혈청 샘플을 사용하여 결정될 수 있다.
바람직한 실시형태에서, 본 발명은 (i) 스파이크(S) 단백질; (ii) 뉴클레오캡시드(N) 단백질; (iii) 막(M) 당단백질; 및/또는 (iv) 엔벨로프(E) 단백질의 천연 형태가 불활성화된 바이러스 입자에 보존된다는 것을 포함한다. 바람직하게는, 불활성화된 SARS-CoV-2 입자는 천연 형태 스파이크(S) 단백질을 포함한다. 따라서, 불활성화된 SARS-CoV-2 입자에서 S(및/또는 N 및/또는 M 및/또는 E) 단백질은 바람직하게는 천연 SARS-CoV-2 입자에 존재하는 하나 이상 또는 모든 (온전한) 면역원성(중화) 에피토프를 포함한다. 바람직하게는, 불활성화된 바이러스 입자에서 S(및/또는 N 및/또는 M 및/또는 E) 단백질은 불활성화 단계에 의해 변형되지 않거나 실질적으로 변형되지 않는다.
바이러스 입자의 표면 형태의 보존은 표준 기술을 사용하여 평가할 수 있다. 예로써, X선 결정학, MS 분석(변형에 의한 아미노산 질량의 이전) 및 저온-전자 현미경과 같은 방법을 사용하여 바이러스 표면을 시각화할 수 있다. 바이러스 입자의 표면에 존재하는 단백질의 2차 및 3차 구조는 원형 이색성(CD) 분광법(예를 들어, 원거리(190-250nm) UV 또는 근거리(250-300nm) UV 범위)과 같은 방법에 의해 분석할 수도 있다. 더욱이, 천연 표면 형태의 보존은, 예를 들어 S 단백질에서 천연 바이러스 표면 상에 존재하는 에피토프에 대한 항체를 사용하여 확인할 수 있다. 따라서 불활성화된 바이러스 입자 및 천연 바이러스 입자 둘 모두와 항-SARS-CoV-2 항체의 반응성은 백신에서 잠재적으로 중화 에피토프의 보유를 입증하는 데 사용될 수 있다.
SARS-CoV-2 비리온 및 특히 스파이크(S) 단백질의 표면 형태가 알려져 있으며 최근 여러 연구에서 발표되었다. 예로써, SARS-CoV-2 수용체 결합 도메인의 결정 구조를 기술하는, Shang, J. et al. (Structural basis of receptor recognition by SARS-CoV-2. Nature https://doi.org/10.1038/s41586-020-2179-y (2020))을 참고한다. 부가하여, Walls et al. (Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein, Cell 180, 1-12 (2020), https://doi.org/10.1016/j.cell.2020.02.058)은 cryo-EM을 사용한 S 단백질 표면 형태의 상세한 설명을 제공하고 보존된 S 단백질 에피토프를 표적화하는 교차-중화 항체를 기술한다.
SARS-CoV-2 표면 에피토프(S 단백질에 포함)에 대한 모노클로날 항체는 문헌(예를 들어, 상기에 언급한 바와 같음)에 기재되어 있으며, 상업적 출처에서 이용가능할 수 있고/있거나 실험 동물의 면역화와 같은 표준 기술을 사용하여 생성될 수 있다. 예를 들어, 2020년 9월 9일 현재, SARS-CoV-2에 대한 최소 169개의 서로 다른 항체가 캘리포니아주 샌디에고 소재의 MyBioSource, Inc.에서 이용가능하다(예를 들어, 카탈로그 번호 MBS8574747, www.MyBioSource.com 참조). 동일한 날짜에 SARS-CoV-2에 대한 최소 28개의 서로 다른 항체가 펜실바니아주 웨인 소재의 Sino Biological US Inc.에서 이용가능하다(예를 들어, 카탈로그 번호 40150-D006, https://www.sinobiological.com/ 참조). 추가 적합한 항체는 Ou et al. (Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV, Nature Communications (2020) 11:1620; https://doi.org/10.1038/s41467-020-15562-9)에 기술되어 있다. 본 발명의 실시형태에서, 숙련가는 이러한 항체와 불활성화된 입자의 교차-반응을 통해 SARS-CoV-2(또는 예를 들어, 그의 S 또는 N 단백질)의 천연 표면 형태의 보존을 검출할 수 있다. 달리 말해서, 불활성화된 입자는 표면 에피토프에 대해 지향된 하나 이상의 항-SARS-CoV-2 항체, 바람직하게는 항-S-단백질 항체, 예를 들어, 천연 SARS-CoV-2 비리온에서 중화 에피토프에 대해 생성된 항체에 특이적으로 결합한다.
백신 조성물에서 SARS-CoV-2 입자는 SARS-CoV-2의 임의의 알려진 균주 또는 이의 변이체로부터 유래될 수 있다. 예로써, 바이러스는 도 2에 정의된 균주일 수 있거나, 그 안에 정의된 뉴클레오티드 또는 아미노산 서열, 또는 여기서 적어도 예를 들어 95% 서열 동일성을 갖는 변이체 서열을 포함할 수 있다. 예로써, 일 실시형태에서 SARS-CoV-2 입자는 서열번호: 1, 9, 12 또는 15에 의해 정의된 바와 같은 DNA 서열에 상응하는 RNA 서열을 포함한다. 바람직한 실시형태에서, SARS-CoV-2 입자는 서열번호: 9에 의해 정의된 DNA 서열에 상응하는 RNA 서열; 즉 이탈리아-INMI1 SARS CoV-2 바이러스를 포함한다. 이 SARS-CoV-2 단리주는 이탈리아 로마 소재의 국립 전염병 연구소 "Lazzaro Spallanzani" IRCCS에서 처음으로 식별되고 특성화되었다(Capobianchi MR, et al. Molecular characterization of SARS-CoV-2 from the first case of COVID-19 in Italy. 2020 Clin Microbiol Infect. 2020 Jul; 26(7): 954-956; doi: 10.1016/j.cmi.2020.03.025). "상응하는"은 정의된 DNA 서열이 바이러스 RNA 서열의 등가물이며, 즉 바이러스 RNA 또는 바이러스 RNA에 상보적인 서열을 인코딩하는 DNA 또는 cDNA 서열인 것으로 이해될 것이다. 본 명세서에 기재된 바와 같이, 불활성화 과정은 바이러스 RNA의 변형(예를 들어, 알킬화 또는 아실화) 및/또는 단편화를 초래할 수 있고, 따라서 불활성화된 바이러스 입자는 본 명세서에 정의된 온전한 RNA 서열을 포함하지 않을 수 있지만 오히려 그러한 서열을 포함하는 천연 바이러스 입자로부터 유래된다는 것으로 이해될 것이다.
SARS-CoV-2 입자는 또한 공지된 SARS-CoV-2 Wuhan-Hu-1 계통의 변이체를 포함하거나 또한, 예를 들어 서열번호: 1 및/또는 NCBI 참조 서열 NC_045512.2에 대해 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 서열인 참조 계통으로 지칭될 수 있다. 바람직하게는, 변이체 서열은 감염성 SARS-CoV-2 입자, 예를 들어 독성 SARS-CoV-2 바이러스를 팩킹할 수 있는 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자를 인코딩한다.
추가로 공지된 SARS-CoV-2 입자는 또한 공지된 SARS-CoV-2 남아프리카 계통 B.1.351의 변이체, 예를 들어 서열번호: 18 및/또는 NCBI 참조 서열 MW598408에 대해 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 서열을 포함할 수 있다. 바람직하게는, 변이체 서열은 감염성 SARS-CoV-2 입자, 예를 들어 독성 SARS-CoV-2 바이러스를 팩킹할 수 있는 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자를 인코딩한다. 공지된 SARS-CoV-2 남아프리카 계통 B.1.351의 변이체의 추가 예는 도 2에 제공되어 있다.
추가로 공지된 SARS-CoV-2 입자는 또한 공지된 SARS-CoV-2 브라질 계통 P.1의 변이체, 예를 들어 서열번호: 20 및/또는 NCBI 참조 서열 MW520923에 대해 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 서열을 포함할 수 있다. 바람직하게는, 변이체 서열은 감염성 SARS-CoV-2 입자, 예를 들어 독성 SARS-CoV-2 바이러스를 팩킹할 수 있는 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자를 인코딩한다. 공지된 SARS-CoV-2 브라질 계통 P.1의 변형의 추가 예는 도 2에 제공되어 있다.
추가로 공지된 SARS-CoV-2 입자는 또한 공지된 SARS-CoV-2 UK 계통 B.1.1.7의 변이체, 예를 들어 서열번호: 22 및/또는 NCBI 참조 서열 MW422256에 대해 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 서열을 포함할 수 있다. 바람직하게는, 변이체 서열은 감염성 SARS-CoV-2 입자, 예를 들어 독성 SARS-CoV-2 바이러스를 팩킹할 수 있는 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자를 인코딩한다. 공지된 SARS-CoV-2 UK 계통 B.1.1.7의 변이체의 추가 예는 도 2에 제공되어 있다.
추가로 공지된 SARS-CoV-2 입자는 또한 공지된 SARS-CoV-2 캘리포니아 계통 B.1.427 및 B.1.429의 변이체, 예를 들어 서열번호: 24 및/또는 서열번호: 26에 대해 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 서열을 포함할 수 있다. 바람직하게, 변이체 서열은 감염성 SARS-CoV-2 입자, 예를 들어 독성 SARS-CoV-2 바이러스를 팩킹할 수 있는 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자를 인코딩한다. 공지된 SARS-CoV-2 캘리포니아 계통의 변형의 추가 예는 GenBank에서 찾을 수 있다.
유사하게, 바람직한 실시형태에서 SARS-CoV-2 입자는 (i) 서열번호: 3에 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 3에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성된 Wuhan 계통의 S 단백질을 포함한다.
추가의 바람직한 실시형태에서 SARS-CoV-2 입자는 (i) 서열번호: 19에 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 19에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성된 남아프리카 B1.351 계통의 S 단백질을 포함한다.
추가의 바람직한 실시형태에서 SARS-CoV-2 입자는 (i) 서열번호: 21에 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 21에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성된 브라질 P.1 계통의 S 단백질을 포함한다.
추가의 바람직한 실시형태에서 SARS-CoV-2 입자는 (i) 서열번호: 23에 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 23에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성된 UK B.1.1.7 계통의 S 단백질을 포함한다.
추가의 바람직한 실시형태에서 SARS-CoV-2 입자는 (i) 서열번호: 25에 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 25에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성된 캘리포니아 B.1.427 계통의 S 단백질을 포함한다.
추가의 바람직한 실시형태에서 SARS-CoV-2 입자는 (i) 서열번호: 27에 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 27에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성된 캘리포니아 B.1.429 계통의 S 단백질을 포함한다.
일부 실시형태에서, 불활성화된 SARS-CoV-2 입자는 백신에서 다른 불활성화된 SARS-CoV-2 입자와 조합된다(다른 = 다른 서열).
일부 실시형태에서, 백신 내 SARS-CoV-2 입자의 조합은 i) 참조 Wuhan_1 계통 예컨대, 예를 들어 서열번호: 1, 9, 12, 15; ii) 남아프리카 B.1.531 계통 예컨대, 예를 들어 서열번호: 18; 브라질 P.1 계통 예컨대, 예를 들어 서열번호: 20; UK B.1.1.7 계통 예컨대, 예를 들어 서열번호: 22 및 캘리포니아 B.1.427 예컨대, 예를 들어 서열번호: 24 또는 B.1.429 계통 예컨대, 예를 들어, 서열번호: 26으로 구성된 군으로부터 선택된 적어도 2개의 SARS-CoV-2 입자를 포함하거나 이로 구성된다. 바람직한 실시형태는 i) Wuhan_1 계통 예컨대, 예를 들어, 서열번호 9; 및 ii) 남아프리카 B.1.531 계통 예컨대, 예를 들어, 서열번호: 18을 포함하는 조합이다.
추가 실시형태에서, 백신 내 SARS-CoV-2 입자의 조합은 i) 참조 Wuhan_1 계통 예컨대, 예를 들어, 서열번호: 1, 9, 12, 15; ii) 남아프리카 B.1.531 계통 예컨대, 예를 들어, 서열번호: 18; 브라질 P.1 계통 예컨대, 예를 들어, 서열번호: 20; UK B.1.1.7 계통 예컨대, 예를 들어, 서열번호: 22 및 캘리포니아 B.1.427 계통 예컨대, 예를 들어, 서열번호: 24 또는 B.1.429 계통 예컨대, 예를 들어, 서열번호: 26으로 구성된 군으로부터 선택된 적어도 3개, 예를 들어 3의 SARS-CoV-2 입자를 포함하거나 이로 구성된다. 이러한 3가 백신의 바람직한 실시형태는 i) Wuhan_1 계통 예컨대, 예를 들어, 서열번호 9; 및 ii) 남아프리카 B.1.531 계통 예컨대, 예를 들어, 서열번호: 18; 및 iii) UK B.1.1.7 계통 예컨대, 예를 들어, 서열번호: 22를 포함하는 조합이다. 이러한 3가 백신의 또 다른 바람직한 실시형태는 i) Wuhan_1 계통 예컨대, 예를 들어, 서열번호 9; 및 ii) 남아프리카 B.1.531 계통 예컨대, 예를 들어, 서열번호: 18; 및 iii) 브라질 P.1 계통 예컨대, 예를 들어, 서열번호: 20을 포함하는 조합이다.
아미노산 서열 및/또는 핵산 서열 간의 유사성은 서열 간의 동일성 매칭 백분율의 관점에서 표현되고, 그렇지 않으면 서열 동일성으로 지칭된다. 서열 동일성은 종종 백분율 동일성의 관점에서 측정된다; 백분율이 높을수록 두 서열이 더 유사하다. 폴리뉴클레오티드 또는 폴리펩티드의 상동체, 이종상동체, 또는 변이체는 표준 방법을 사용하여 정렬될 때 상대적으로 높은 정도의 서열 동일성을 가질 것이다.
비교를 위한 서열의 정렬 방법은 당업계에 잘 알려져 있다. 다양한 프로그램 및 정렬 알고리즘은 Smith & Waterman, Adv. Appl. Math. 2:482, 1981; Needleman & Wunsch, Mol. Biol. 48:443, 1970; Pearson & Lipman, Proc. Natl. Acad. Sci. USA 85:2444, 1988; Higgins & Sharp, Gene, 73:237-44, 1988; Higgins & Sharp, CABIOS 5: 151-3, 1989; Corpet et al., Nuc. Acids Res. 16: 10881-90, 1988; Huang et al. Computer Appls. in the Biosciences 8, 155-65, 1992; 및 Pearson et al., Meth. Mol. Bio. 24:307-31, 1994에 기술되어 있다. Altschul et al, J. Mol. Biol. 215:403-10, 1990은 서열 정렬 방법 및 상동성 계산에 대한 자세한 고려사항을 제시한다.
일단 정렬되면, 일치하는 수는 동일한 뉴클레오티드 또는 아미노산 잔기가 양 서열에 존재하는 위치의 수를 계수함에 의해 결정된다. 서열 동일성 퍼센트는 일치하는 수를 식별된 서열에 제시된 서열의 길이 또는 연결 길이(예컨대 식별된 서열에 제시된 서열로부터 100개 연속 뉴클레오티드 또는 아미노산 잔기)로 나누고, 그 다음 결과 값에 100을 곱함에 의해 결정된다. 바람직하게, 서열 동일성 백분율은 서열의 전체 길이에 걸쳐 결정된다. 예를 들어, 1166개의 일치를 갖는 펩티드 서열은 1554개의 아미노산을 갖는 테스트 서열과 정렬될 때 테스트 서열과 75.0% 동일하다(1166÷1554*100=75.0). 퍼센트 서열 동일성 값은 가장 가까운 10분의 1로 반올림된다. 예를 들어 75.11, 75.12, 75.13 및 75.14는 75.1로 반내림하고 반면 75.15, 75.16, 75.17, 75.18 및 75.19는 75.2로 반올림한다. 길이 값은 항상 정수일 것이다.
서열 분석 프로그램 BLASTP, BLASTN, BLASTX, TBLASTN 및 TBLASTX와 관련하여 사용하기 위한 NCBI Basic Local Alignment Search Tool(BLAST)(Altschul et al., Mol. Biol. 215:403, 1990)은 국립 생명공학 정보 센터(NCBI, 메릴랜드주 베데스다 소재) 및 인터넷을 포함한 여러 출처에서 이용가능하다. 이 프로그램을 사용하여 서열 동일성을 결정하는 방법에 대한 설명은 인터넷 상 NCBI 웹사이트에서 이용가능하다. BLAST 및 BLAST 2.0 알고리즘은 또한 Altschul et al., Nucleic Acids Res. 25:3389-3402, 1977에 기술되어 있다. BLAST 분석을 수행하기 위한 소프트웨어는 국립 생명공학 정보 센터(ncbi.nlm.nih.gov)를 통해 공개적으로 이용가능하다. BLASTN 프로그램(뉴클레오티드 서열용)은 기본적으로 단어 길이(W) 11, 정렬(B) 50, 기대값(E) 10, M=5, N=-4 및 양 가닥의 비교를 사용한다. BLASTP 프로그램(아미노산 서열용)은 기본값으로 단어 길이(W) 3, 기대값(E) 10, 및 BLOSUM62 스코어링 매트릭스를 사용한다(Henikoff & Henikoff, Proc. Natl. Acad. Sci. USA 89: 10915, 1989 참조).
폴리뉴클레오티드 또는 폴리펩티드의 상동체 및 변이체는 전형적으로 참조의 적어도 50, 100, 150, 250, 500, 1000, 2000, 5000 또는 10,000개의 뉴클레오티드 또는 아미노산 잔기에 걸쳐, 참조 서열의 전장에 걸쳐 또는 관심있는 참조 아미노산 서열과의 전장 정렬에 걸쳐 계수된 적어도 약 75%, 예를 들어 적어도 약 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 또는 99% 서열 동일성의 소유를 특징으로 한다. 참조 서열과 훨씬 더 큰 유사성을 가진 폴리뉴클레오티드 또는 단백질은 이 방법에 의해 평가할 때 증가하는 동일성 백분율 예컨대 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95%, 적어도 98%, 또는 적어도 99% 서열 동일성을 나타낼 것이다. 아미노산 또는 핵산 서열의 서열 비교를 위해, 전형적으로 하나의 서열은 테스트 서열이 비교되는 참조 서열로 작용한다. 서열 비교 알고리즘을 사용하는 경우, 테스트 서열과 참조 서열을 컴퓨터에 입력하고 필요에 따라 하위 서열 좌표를 지정하고 서열 알고리즘 프로그램 매개변수를 지정한다. 기본 프로그램 매개변수가 사용된다.
유용한 알고리즘의 일 예는 PILEUP이고, 이는 Feng & Doolittle, Mol. Evol. 35:351-360, 1987의 점진적 정렬 방법의 단순화를 사용한다. 사용된 방법은 Higgins & Sharp, CABIOS 5:151-153, 1989에 의해 기술된 방법과 유사하다. PILEUP을 사용하여 참조 서열을 다른 테스트 서열과 비교하여 다음 매개변수를 사용하여 퍼센트 서열 동일성 상관관계를 결정한다: 기본 간격 가중치(3.00), 기본 간격 길이 가중치(0.10) 및 가중치가 적용된 말단 간격. PILEUP은 GCG 서열 분석 소프트웨어 패키지, 예를 들어 버전 7.0으로부터 얻을 수 있다(Devereaux et al., Nuc. Acids Res. 12:387-395, 1984).
본 명세서에 사용된 "적어도 80% 동일성"에 대한 언급은 특정 참조 서열에 대해, 예를 들어, 참조 서열의 적어도 50, 100, 150, 250, 500, 1000, 5000 또는 10,000개 뉴클레오티드 또는 아미노산 잔기에 대해 또는 서열의 전장에 대해 적어도 80%, 적어도 85%, 적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96%, 적어도 97%, 적어도 98%, 적어도 99%, 또는 심지어 100% 동일성을 지칭한다. 본 명세서에 사용된 "적어도 90% 동일성"에 대한 언급은 특정 참조 서열에 대해, 예를 들어, 참조 서열의 적어도 50, 100, 150, 250, 500, 1000, 5000 또는 10,000개 뉴클레오티드 또는 아미노산 잔기에 대해 또는 서열의 전장에 대해 "적어도 90%, 적어도 91%, 적어도 92%, 적어도 93%, 적어도 94%, 적어도 95%, 적어도 96% , 적어도 97%, 적어도 98%, 적어도 99%, 또는 심지어 100% 동일성"을 지칭한다.
SARS-CoV-2 백신은 상술한 바와 같이 천연 SARS-CoV-2 입자의 불활성화의 단계를 포함하는 방법에 의해 생성될 수 있다. 일반적으로 천연 SARS-CoV-2 입자는 표준 배양 방법, 예를 들어 바람직하게는 Vero 세포를 사용하여 포유동물 세포에서 시험관내 생성에 의해에 의해 수득될 수 있다. 예로써, 천연 SARS-CoV-2 입자는 예를 들어 (그의 내용이 그 전체로 본 명세서에 포함된) WO 2017/109225 및/또는 WO 2019/057793에 기재된 것과 유사한 방법을 사용하여 생성될 수 있으며, 이는 Vero 세포에서 지카 및 치쿤구니야 바이러스의 생성에 대한 방법을 기술한다. 이들 문헌에 기술된 계대, 수확, 침전, 투석, 여과 및 정제와 같은 단계는 SARS-CoV-2 입자를 생성하는 현재 공정에 동일하게 적용할 수 있다.
예로써, 일부 실시형태에서, 방법은 (i) 수크로스 밀도 구배 원심분리, (ii) 리간드-활성화 코어 및 기공을 포함한 불활성 쉘을 포함하는 컬럼에 패킹된 고체-상 매트릭스로서, 여기서 기공의 분자량 컷-오프는 바이러스 입자가 리간드-활성화된 코어로 들어가는 것을 배제하고, 여기서 기공의 분자량 컷-오프보다 더 작은 분자는 리간드-활성화된 코어에 들어갈 수 있고 바이러스 입자 수집하는, 매트릭스, 및/또는 (iii) 배치 또는 크기 배제 크로마토그래피와 같은 하나 이상의 크기 배제 방법에 의해 불활성화된 SARS-CoV-2 입자를 정제하는 것을 포함하여; 정제된 불활성화된 SARS-CoV-2 입자를 얻을 수 있다. 바람직하게는, 바이러스 입자의 생성된 정제된 제제에서, (i) 잔류 숙주 세포 DNA의 농도는 100ng/mL 미만이고; (ii) 잔류 숙주 세포 단백질의 농도는 1μg/mL 미만이고; (iii) 감염성 바이러스 입자의 잔류 응집체 농도는 1μg/mL 미만이다.
일부 실시형태에서, 방법은 SARS-CoV-2 입자를 포함하는 수확된 배양 배지를 침전시키고 이에 의해 상등액에서 천연 SARS-CoV-2 입자를 생성하는 단계를 포함할 수 있다. 침전 단계는 배양 배지를 프로타민 설페이트 또는 벤조나아제와 접촉시키는 것을 포함할 수 있다. 이러한 단계를 사용함에 의해, 숙주 세포에서 유래한 오염 DNA뿐만 아니라 미성숙 및 그렇지 않으면 비-감염성 바이러스 입자 둘 모두가 제제로부터 분리될 수 있다. 더욱이, 프로타민 설페이트는 바이러스 분획으로부터, 예를 들어 수크로스 밀도 원심분리 또는 리간드-활성화된 코어 및 기공을 포함한 불활성 쉘을 포함하는 컬럼에 패킹된 고체-상 매트릭스를 사용하여 매우 효율적으로 분리될 수 있으며, 여기서 기공은 바이러스 입자가 리간드-활성화된 코어에 들어가는 것을 배제하는 분자량 컷-오프를 포함하고, 기공의 분자량 컷-오프보다 작은 분자(예를 들어, 프로타민 설페이트)가 리간드-활성화된 코어에 들어갈 수 있어 보다 안전한 백신을 고수율로 생성할 수 있다.
따라서 수득된 바이러스 제제 또는 백신의 잔류 숙주 세포 DNA는 1μg/mL 미만, 특히 900, 800, 700, 600, 500, 400, 300 또는 200ng/mL 미만, 바람직하게는 150 또는 100ng/mL 미만일 수 있다. 바람직한 실시형태에서, 바이러스 제제 또는 백신의 잔류 숙주 세포 DNA는 40pg/mL 미만이다. 일부 실시형태에서, 바이러스 제제 또는 백신의 잔류 숙주 세포 단백질은 10μg/mL 미만, 특히 9, 8, 7, 6, 5, 4, 3 또는 2μg/mL 미만, 바람직하게는 1μg/mL 미만이다. 바람직한 실시형태에서, 바이러스 제제 또는 백신의 잔류 숙주 세포 단백질은 150ng/mL 미만이다. 일부 실시형태에서, 바이러스 제제 또는 백신의 잔류 비-감염성 바이러스 입자는 10μg/mL 미만, 특히 9, 8, 7, 6, 5, 4, 3 또는 2μg/mL 미만, 바람직하게는 1μg/mL 미만이다. 바람직한 실시형태에서, 바이러스 제제 또는 백신의 잔류 비-감염성 바이러스 입자의 함량은 100ng/mL 미만이다.
일부 실시형태에서, 백신 및/또는 SARS-CoV-2 입자는 잔류 프로타민(예를 들어, 프로타민 설페이트)을 전형적으로 미량으로 포함할 수 있다. 일부 실시형태에서, 바이러스 제제 또는 백신에서 잔류 프로타민(예를 들어, 프로타민 설페이트)은 2μg/mL 또는 1μg/mL 미만, 특히 900, 800, 700, 600, 500, 400, 300 또는 200ng/mL 미만, 바람직하게는 100ng/mL 미만이고, 더욱 바람직하게는 HPLC의 검출 한계 미만, 특히 최종 약물 물질에서의 검출 한계 미만이다. 일부 실시형태에서, PS 함량은 HPLC 또는 크기 배제 크로마토그래피(SEC)에 의해 시험된다. 예를 들어, HPLC는 일상적인 릴리스 검정으로 JEV 수크로스 구배 풀 샘플에서 PS 측정을 위해 검증되었고 매우 민감성이다(즉, 정량 한계(LOQ) 3μg/mL; 검출 한계(LOD) 1μg/mL). 본 발명에서, SARS-CoV-2 약물 물질의 PS 함량은 <LOD였다. 일 실시형태에서, PS 함량의 HPLC 평가는 30% 아세토니트릴, 0.1% 트리플루오로아세트산을 용매로서 0.6ml/분의 유속으로 25℃ 및 214nm에서 검출을 사용하여 Superdex Peptide 10/300GL 컬럼(GE: 17-5176-01)에서 수행할 수 있다. 정제된 바이러스 제제에서 잔류 프로타민에 대한 보다 민감한 측정 방법은 질량 분석법(MS)이다. 일부 실시형태에서, 지카 바이러스 제제에서 잔류 PS 수준은 MS 또는 기타 그러한 고도로 민감한 방법, 예를 들어 핵 자기 공명(NMR)에 의해 시험된다. 이 방법을 사용하면 잔류 PS뿐만 아니라 PS의 단편 및/또는 분해 생성물을 미량, 예컨대 전형적인 샘플 장입당 106, 107 또는 108개 분자만큼 낮은 수준으로 검출할 수 있다. 일부 실시형태에서, PS 수준은 의약품에서 시험된다. 일부 실시형태에서, PS 수준은 약물 물질에서 시험된다.
바람직하게는 의약품 또는 약물 물질(예를 들어, 백신 조성물)에서 불활성화제(예를 들어, 베타-프로피오락톤)의 양은 매우 낮으며, 예를 들어 100ppm 미만, 10ppm 미만, 또는 1ppm 미만(중량 기준)이다.
SARS-CoV-2 백신은 대상체, 바람직하게는 포유동물 대상체, 보다 바람직하게는 인간 대상체에게 투여될 수 있다. 전형적으로 SARS-CoV-2 백신은 예를 들어 SARS-CoV-2 감염을 예방하고/하거나 SARS-CoV-2 연관된 질환(COVID-19)을 예방하기 위해 SARS-CoV-2 감염의 위험이 있는 대상체에게 투여된다. 대상체는 바람직하게는 (i) 노인 대상체(예를 들어, 65세, 70세 또는 80세 초과의 노인) (ii) 임신한 대상체 (iii) 면역저하 대상체 또는 (iv) 어린이(예를 들어, 18세, 16세, 14세, 12세, 10세, 8세, 6세, 4세, 2세 이하의 사람)이다. 본 명세서에 기술된 SARS-CoV-2 백신은 유리하게 SARS-CoV-2-이환율 또는 사망률에 특히 민감하거나 취약한 대상체, 즉 면역저하, 임산부 또는 노인 대상체에서 강력한 면역 반응을 생성할 수 있다. SARS-CoV-2 백신은 단일 용량 또는 2회 이상의 용량, 예를 들어 약 7, 14, 21 또는 28일의 간격으로 분리된 용량으로 대상체에게 투여될 수 있다.
바람직한 실시형태에서, 인간 대상체에 투여시 백신은 백신 매개된 질환 증강(잠재적으로 항체의존 면역증강을 통함)을 유도하지 않는다. 백신-매개된 질환 증강은 대상체가 나중에 천연 바이러스에 감염되는 경우 증가된 질환 중증도를 초래하는 백신을 특징으로 한다. 본 명세서에 기술된 불활성화된 SARS-CoV-2 백신이 인간 대상체에서 백신 매개된 질환 증강을 촉진하지 않고, 따라서 대량 백신접종 목적을 위해 안전하게 사용될 수 있다는 것이 본 발명의 이점이다. 특히, 본 명세서에 기재된 백신은 고품질 면역원성 에피토프를 보유하고, 따라서 높은 중화 항체 역가를 초래하고 대상체에 투여시 백신 매개된 질환 증강의 위험을 감소시킨다. 백신 매개된 질환 증강 발달의 위험은 비-인간 영장류에서 평가될 수 있다. 이와 관련하여 지침은 CEPI/BC 2020년 3월 12-13일 회의에 대한 합의 요약 보고서: Assessment of risk of disease enhancement with COVID-19 vaccines (Lambert, P-H, et al. 2020, doi: 10.1016/j.vaccine.2020.05.064)에 제공되어 있다.
또 다른 바람직한 실시형태에서, 인간 대상체에 투여시 백신은 면역병리를 초래하지 않는다. 마우스에서, SARS-CoV-1 백신 유도된 면역병리는 백신 항원에 대한 우세한 Th2 유형 반응의 결과로 간주되었다(Tseng et al., 2012, 상기 참조). 본 발명의 실시형태에서, Th1 유형 반응은, 예를 들어 Th1-지향 어쥬번트 CpG의 사용에 의하여 고양되거나 선호된다. 면역병리 전개의 위험은, 예를 들어 Tseng C.T. et al. (2012) PLoS ONE 7(4):e35421에 기술된 바와 같이 동물 모델에서 평가될 수 있다.
본 명세서에 기재된 임의의 SARS-CoV-2 백신 또는 조성물은 치료적으로 유효한 양으로 또는 치료적으로 유효한 양의 용량으로 대상체에게 투여될 수 있다. 본 명세서에 사용된 바와 같이, 백신의 "치료적으로 유효한 양"은 SARS-CoV-2에 대한 감염의 예방, 면역 반응 또는 강화된 면역 반응 또는 SARS-CoV-2 질환과 연관된 증상의 예방 또는 감소를 포함하지만 이에 제한되지 않는 본 명세서에 기재된 것과 같은 대상체에서 원하는 반응 또는 결과를 초래하는 임의의 양이다.
일부 실시형태에서, 본 명세서에 기재된 SARS-CoV-2 백신 또는 조성물의 치료적으로 유효한 양 또는 예방적으로 유효한 양(투여량)은 항원-특이적 항체(예를 들어, 항-SARS-CoV-2 항체)를 생성하기에 충분한 양이다. 일부 실시형태에서, 치료적으로 또는 예방적으로 유효한 양은 적어도 70% 확률로 대상체를 혈청전환시키기에 충분하다. 일부 실시형태에서, 치료적으로 또는 예방적으로 유효한 양은 적어도 75%, 80%, 85% 90%, 95%, 96%, 97%, 98%, 또는 적어도 99% 확률로 대상체를 혈청전환시키기에 충분하다. 대상체가 혈청전환되었는지 여부는 대상체로부터 혈청 샘플을 수득하고 항-SARS-CoV-2 항체를 검출하기 위한 검정을 수행하는 것과 같은 당업계에 공지된 임의의 방법에 의해 평가될 수 있다. 일부 실시형태에서, 대상체로부터의 혈청 샘플이 역치 또는 미리결정된 기준선을 초과하는 양의 항-SARS-CoV-2 항체를 함유하는 경우 대상체는 혈청전환된다. 대상체로부터의 혈청 샘플에 존재하는 항-SARS-CoV-2 항체(즉, 항-SARS-CoV-2 S 단백질 IgG 항체)가 동일한 대상체로부터 이전에 채취한 혈청 샘플에 비해 적어도 4-배 증가가 있는 경우 대상체는 일반적으로 혈청전환된 것으로 간주된다.
일 실시형태에서, 본 발명의 백신 조성물에서 불활성화된 SARS-CoV-2 바이러스의 용량은 약 0.01 내지 25mAU(SEC-HPLC에 의해 평가된 밀리-흡수 단위 x 분), 바람직하게는 약 0.05 내지 10mAU, 보다 바람직하게는 약 0.1 내지 5mAU, 가장 바람직하게는 약 0.25 내지 2.5mAU이다. 일 실시형태에서, 용량은 (μ)BCA 검정에 의해 측정될 때 약 0.05 내지 50μg 총 단백질, 약 0.1 내지 25μg, 약 0.25 내지 12.5μg, 바람직하게는 약 0.5 내지 5μg 총 단백질이다. 보다 바람직하게는 백신 조성물에서 불활성화된 SARS-CoV-2 바이러스의 용량은 적어도 2.5μg 총 단백질, 적어도 3.5μg 총 단백질 또는 적어도 2.5μg 총 단백질이며, 예를 들어 백신 조성물은 2.5μg 내지 25μg, 3.5μg 내지 10μg 또는 4μg 내지 6μg 총 단백질/용량, 바람직하게는 약 5μg 총 단백질/용량을 포함한다. 일부 실시형태에서, 투여량은, 예를 들어 ELISA에 의해 분석된 바와 같이, 불활성화된 SARS-CoV-2 제형에서 S 단백질의 총량에 의해 결정된다. 항원의 질량은 또한 대략 2μg/ml 총 표면 단백질 및 대략 1μg/mL S-단백질인 것으로 추정되는 용량 당량당 SE-HPLC 피크 면적(밀리-흡수 단위 x 분으로 기록됨; mAU)을 분석함에 의해 평가될 수 있다. 일 실시형태에서, 용량은 ELISA에 의해 측정될 때 약 0.025 내지 25μg S-단백질, 약 0.05 내지 12.5μg, 약 0.125 내지 6.25μg, 바람직하게는 약 0.25 내지 2.5μg S-단백질이다.
바람직한 실시형태에서, SARS-CoV-2 백신에서 항원의 양은 ELISA에 의해 결정된다. 일 실시형태에서, ELISA는 SARS-CoV-2 단백질 또는 단백질의 일부, 예를 들어, 뉴클레오캡시드(N), 막(M) 또는 스파이크(S) 단백질을 측정하며; 즉, ELISA는 SARS-CoV-2 단백질 또는 단백질의 일부에 특이적인 코팅 항체를 이용한다. 바람직한 실시형태에서, 코팅 항체는 SARS-CoV-2 스파이크 단백질 S1 서브유닛, 예를 들어, 서열번호:3, 19, 21, 23, 25 또는 27의 S-단백질 서열의 잔기 14-685(또는 14-683), 또는 수용체 결합 도메인(RBD), 예를 들어 서열번호:3, 19, 21, 23, 25 또는 27의 S-단백질 서열의 잔기 331 내지 528(또는 319 내지 541)에 특이적이다(도 13 참조). 일 실시형태에서, ELISA 판독은 검출된 단백질의 단위 측정당 질량, 예를 들어, μg/mL S-단백질이다. 바람직한 실시형태에서, 사용된 표준은 스파이크 단백질 삼량체이고 SARS-CoV-2 ELISA의 결과는 표준 단백질의 ACE-2 결합 능력에 상응하는 "항원 단위"(AU)로 보고된다(제조업체에 의해 결정됨).
일 실시형태에서, 대상체에게 투여되는 SARS-CoV-2 항원의 양은 약 1 내지 100AU/용량, 바람직하게는 약 2 내지 75AU/용량, 바람직하게는 약 3 내지 60AU/용량, 보다 바람직하게는 약 3 내지 55AU/용량, 보다 바람직하게는 약 3 내지 53AU/용량이다. 훨씬 더 바람직한 실시형태에서, 대상체에게 투여되는 SARS-CoV-2 항원의 양은 용량당 3AU, 10AU 또는 40AU이고, 가장 바람직하게는 용량당 40AU이다. 추가의 바람직한 실시형태에서, 대상체에게 투여되는 SARS-CoV-2 항원의 양은 적어도 10AU/용량, 적어도 20AU/용량, 적어도 25AU/용량 또는 적어도 30AU/용량, 예를 들어 약 10 내지 60AU/용량, 20 내지 50AU/용량, 25 내지 45AU/용량 또는 30 내지 40AU/용량, 예를 들어 약 35AU/용량이다. SARS-CoV-2 항원의 양(예를 들어, AU/용량 단위)은 예를 들어 실시예 1에 기술된 바와 같이 SARS-CoV-2 ELISA 검정에 의해 평가될 수 있다. AU당 약 1 내지 1.5 x 107 바이러스 입자가 있고, 그리고 상기에서 기술된 SARS-CoV-2 항원의 양은 그에 따라 해석될 수 있다고 추정된다. 따라서 일부 실시형태에서, 대상체에게 투여되는 SARS-CoV-2 항원의 양은 약 1.5 x 107 내지 1.5 x 109 바이러스 입자/용량, 또는 약 4.5 x 107 내지 9.0 x 108 바이러스 입자/용량, 예를 들어 적어도 1.5 x 108 바이러스 입자/용량 또는 적어도 3.0 x 108 바이러스 입자/용량, 약 1.5 x 108 내지 7.5 x 108 바이러스 입자/용량 또는 약 4.5 x 108 내지 6.0 x 108 바이러스 입자/용량이다.
일부 실시형태에서, 대상체의 혈청전환은 플라크 감소 중화 시험(PRNT)을 수행함에 의해 평가된다. 간단히 말해서, PRNT는 대조군 혈청/항체와 비교하여 SARS-CoV-2 플라크의 수를 50%(PRNT50)까지 감소시키는데 필요한 혈청 역가를 결정하는데 사용된다. PRNT50은 Vero 세포의 단층 또는 SARS-CoV-2에 감염될 수 있는 임의의 다른 세포 유형/주를 사용하여 수행될 수 있다. 대상체로부터의 혈청을 희석하고 살아있는 비-불활성화된 SARS-CoV-2와 함께 인큐베이션한다. 혈청/바이러스 혼합물을 Vero 세포에 적용하고 일정 기간 동안 인큐베이션할 수 있다. Vero 세포 단층 상에 형성된 플라크를 계수하고 혈청 또는 대조군 항체가 없는 SARS-CoV-2에 의해 형성된 플라크의 수와 비교한다. 가이드라인으로, PRNT50에서 혈청의 1:10 희석의 중화 항체의 역치는 일반적으로 JEV의 경우에 보호의 증거로 인정된다(Hombach et. al. Vaccine (2005) 23:5205-5211).
일부 실시형태에서, SARS-CoV-2 입자는 약학적 조성물과 같은 조성물로 투여하기 위해 제형화될 수 있다. 본 명세서에 사용된 용어 "약학적 조성물"은 불활성화된 SARS-CoV-2와 같은 적어도 하나의 활성 성분과 하나 이상의 약학적으로 허용되는 부형제를 포함할 수 있는 하나 이상의 불활성 성분의 혼합 또는 조합으로부터 생성된 생성물을 의미한다. 바람직한 약학적으로 허용가능한 부형제는 인간 혈청 알부민(HSA), 예컨대 특히 재조합 HSA(rHSA)이다. 일 실시형태에서, 발명의 SARS-CoV-2 백신은 약 10 내지 50μg HSA/용량, 바람직하게는 약 20 내지 40μg HSA/용량, 더욱 바람직하게는 약 25 내지 35μg HSA를 함유한다.
백신을 포함하는 발명의 약학적 조성물은 당업계에 잘 알려져 있고 일상적으로 실시되는 방법에 따라 제조될 수 있다(예를 들어, Remington: The Science and Practice of Pharmacy, Mack Publishing Co. 20th ed. 2000; 및 Ingredients of Vaccines - Fact Sheet from the Centers for Disease Control and Prevention 참조, 예를 들어, 백신을 그 작용을 개선하는 데 도움이 되는 상기에 기술된 어쥬번트 및 강화제, 백신이 변경되지 않은 상태로 유지되는 데 도움이 되는 보존제 및 안정화제(예를 들어 알부민, 페놀, 글리신)). 본 명세서에 사용된 용어 "백신"은 면역원성 조성물, 예를 들어, 항원(예를 들어 SARS-CoV-2 항원)에 대해 (인간) 대상체에서 면역 반응을 유도할 수 있는 조성물을 지칭한다. 예로써, 백신 또는 조성물은, 예를 들어 본 명세서에 기술된 검정(예를 들어 미세중화 검정)에서 결정된 바와 같이 SARS-CoV-2에 대한 중화 항체를 생성할 수 있다. 일부 실시형태에서, 백신 또는 조성물은, 예를 들어, 본 명세서에 기재된 바와 같은 S-단백질 IgG ELISA 검정에 의해 검출된 바와 같이, SARS-CoV-2 S(스파이크) 단백질에 대한 항체(예를 들어, IgG)를 생성할 수 있다. 일부 실시형태에서, 백신 또는 조성물은, 예를 들어, (예를 들어 IFN-γ 생산에 기반하여) 본 명세서에 기재된 바와 같은 ELISPOT 검정에 의해 검출된 바와 같이, SARS-CoV-2 단백질 또는 펩티드에 대한 T 세포 반응, 예로써 SARS-CoV-2 S-단백질, 막(M) 단백질 및/또는 이로부터 유래된 뉴클레오캡시드(N) 단백질 또는 펩티드에 대한 T 세포 반응을 생성할 수 있다. 바람직하게는 백신 또는 면역원성 조성물은 SARS-CoV-2에 대한 중화 항체 및 T 세포 반응을 생성한다. 전형적으로 백신 또는 면역원성 조성물은 항원에 의해 야기되는 질환에 대한 보호 효과, 예를 들어 SARS-CoV-2 감염(예를 들어 증상 및/또는 무증상 감염) 및/또는 COVID-19 질환)에 대한 보호 효과를 유도할 수 있다.
약학적 조성물은 바람직하게는 GMP 조건 하에서 제조된다. 전형적으로, 불활성화된 SARS-CoV-2 백신 제제의 치료학적으로 또는 예방적으로 유효한 용량은 발명의 약학적 조성물에 이용된다. 불활성화된 SARS-CoV-2 입자는 당업자에게 공지된 통상적인 방법에 의해 약학적으로 허용가능한 투여 형태로 제형화된다. 최적의 원하는 반응(예를 들어, 예방적 반응)을 제공하도록 투여량 요법을 조정한다.
본 발명의 약학적 조성물에서 활성 성분의 투여량은 대상체에 대해 독성 없이 특정 대상체, 조성물 및 투여의 방식에 대해 원하는 약학적 반응을 달성하는 데 효과적인 활성 성분의 양을 얻도록 다양할 수 있다. 선택된 투여량 수준은 이용된 본 발명의 특정 조성물의 활성, 투여의 경로, 투여의 시간, 이용되는 특정 화합물의 배설의 속도, 치료의 기간, 이용된 특정 조성물과 조합하여 사용되는 기타 약물, 화합물 및/또는 물질, 연령, 성별, 체중, 병태, 전반적인 건강 및 치료되는 대상체의 이전 병력 등을 포함한 다양한 약동학적 인자에 의존한다.
의사, 수의사 또는 기타 훈련된 개업의는 원하는 치료 또는 예방 효과를 달성하는데 필요한 수준보다 낮은 수준에서 약학적 조성물에 이용된 불활성화된 SARS-CoV-2 백신의 투여를 시작할 수 있고 원하는 효과(예를 들어, 항-SARS-CoV-2 바이러스 항체의 생성)이 달성될 때까지 투여량을 증가시킬 수 있다. 일반적으로, 본 명세서에 기재된 바와 같은 인간의 그룹의 예방적 치료를 위한 본 발명의 조성물의 유효한 용량은 투여의 수단, 표적 부위, 환자의 생리학적 상태, 환자가 인간인지 또는 동물인지 여부, 투여된 다른 약물 및 원하는 항-SARS-CoV-2 항체의 역가를 포함하는 많은 상이한 인자에 따라 달라진다. 안전성과 효능을 최적화하기 위해 투여량을 적정해야 한다. 일부 실시형태에서, 투여 요법은 0일차에 1회 및 약 7일차에 1회인, 2번의 불활성화된 SARS-CoV-2 백신의 용량의 피하 또는 근육내 투여를 수반한다. 일부 실시형태에서, 투여 요법은 0일차에 1회 및 약 14일차에 1회인, 2번의 불활성화된 SARS-CoV-2 백신의 용량의 피하 투여를 수반한다. 일부 실시형태에서, 투여 요법은 0일차에 1회 및 약 21일차에 1회인, 2번의 불활성화된 SARS-CoV-2 백신의 용량의 피하 투여를 수반한다. 일부 실시형태에서, 투여 요법은 0일차에 1회 및 약 28일차에 1회인, 2번의 불활성화된 SARS-CoV-2 백신의 용량의 피하 투여를 수반한다. 일부 실시형태에서, 불활성화된 SARS-CoV-2 백신은 대상체에게 1회 투여된다. 바람직한 실시형태에서, SARS-CoV-2 백신은 대상체에게 1회 초과, 바람직하게는 2회 투여된다. 바람직한 실시형태에서, 백신은 0일차 및 21일차에 투여된다. 또 다른 바람직한 실시형태에서, 백신은 0일차 및 28일차에 투여된다.
추가 실시형태에서, 불활성화된 SARS-CoV-2 백신의 제1(프라임) 용량이 투여되고, 불활성화된 SARS-CoV-2 백신의 제2(부스트) 용량이 제1 용량 후 적어도 28일, 적어도 60일, 적어도 70일, 적어도 80일 또는 90일에 투여된다. 따라서 일부 실시형태에서, 불활성화된 SARS-CoV-2 백신의 제2 용량은 제1 용량 후 30 내지 120일 또는 1 내지 4개월(바람직하게는 약 3개월)에 투여된다.
다른 실시형태에서, 불활성화된 SARS-CoV-2 백신은 부스트 용량으로서만 투여되며, 예를 들어 (다른) SARS-CoV-2 백신의 제1(프라임) 용량이 투여된 다음 불활성화된 SARS-CoV-2 백신의 제2(부스트) 용량이 예를 들어 제1 용량 후 적어도 7, 14, 28, 60 또는 90일에 투여된다. SARS-CoV-2 백신의 제1(프라임) 용량은 SARS-CoV-2 바이러스에 대한 대상체에서 면역 반응 및/또는 보호 효과를 자극하는 임의의 다른 백신 또는 면역원성 조성물을 포함할 수 있다. 예를 들어, SARS-CoV-2 백신의 제1 용량은 재조합 바이러스 벡터 또는 하나 이상의 SARS-CoV-2 단백질 및/또는 이의 단편, 예를 들어 SARS-CoV-2 스파이크(S) 단백질을 인코딩하는 mRNA 서열을 포함할 수 있다. 대안적으로 SARS-CoV-2 백신의 제1 용량은, 예를 들어 하나 이상의 SARS-CoV-2 단백질 및/또는 이의 단편, 예를 들어 SARS-CoV-2 스파이크(S) 단백질 또는 이의 단편을 포함하는 서브유닛 백신을 포함할 수 있다.
또한, 예를 들어 SARS-CoV-2 감염의 중증도를 예방하거나 감소시키기 위해 대상체에 예방적 투여에 사용하기 위한 키트가 본 개시내용의 범주 내에 있다. 이러한 키트는 불활성화된 SARS-CoV-2 백신과 같은 불활성화된 SARS-CoV-2를 함유하는 조성물을 포함하는 하나 이상의 용기를 포함할 수 있다. 일부 실시형태에서, 키트는 제2 조성물, 예컨대 제2 백신, 예를 들어, 제1 용량에서와 상이한 기술을 적용한 SARS-CoV-2 백신의 제2 종류를 포함하는 하나 이상의 추가 성분을 추가로 포함할 수 있다. 일부 실시형태에서, 제2 백신은 아르보바이러스에 대한 백신이다. 일부 실시형태에서, 제2 백신은 일본 뇌염 바이러스 백신, 지카 바이러스 백신, 뎅기열 바이러스 백신 및/또는 치쿤구니야 바이러스 백신이다.
일부 실시형태에서, 키트는 본 명세서에 기재된 임의의 방법에 따른 사용 설명서를 포함한다. 포함된 설명서는 SARS-CoV-2 감염을 예방하거나 발병을 지연시키거나 중증도를 감소시키기 위한 불활성화된 SARS-CoV-2 백신을 함유하는 조성물의 투여에 대한 설명을 포함할 수 있다. 키트는 대상체가 SARS-CoV-2에 노출되거나 SARS-CoV-2 감염에 걸릴 위험이 있는지 여부를 식별하는 것을 기반으로 하여 투여에 적합한 대상체 선택하는 설명을 추가로 포함할 수 있다. 또 다른 실시형태에서, 설명서는 SARS-CoV-2에 노출되거나 SARS-CoV-2 감염에 걸릴 위험이 있는 대상체에게 불활성화된 SARS-CoV-2 백신을 함유하는 조성물을 투여하는 것에 대한 설명을 포함한다.
불활성화된 SARS-CoV-2 백신을 함유하는 조성물의 사용과 관련된 설명서에는 일반적으로 의도된 치료를 위한 투여량, 복용 일정 및 투여의 경로에 대한 정보가 포함된다. 용기는 단위 용량, 벌크 패키지(예를 들어, 다중-용량 패키지) 또는 하위-단위 용량일 수 있다. 발명의 키트에 제공된 설명서는 전형적으로 라벨 또는 패키지 삽입물(예를 들어, 키트에 포함된 종이 시트)에 작성된 설명서이지만 기계-판독가능한 설명서도 허용가능하다.
본 개시내용의 키트는 적합한 포장에 있다. 적합한 포장은 바이알, 병, 항아리, 연포장 등을 포함하지만 이에 제한되지 않는다. 또한 주사기 또는 주입 장치와 같은 특정 장치와 조합하여 사용하기 위한 패키지가 고려된다. 용기는 멸균 접근 포트를 가질 수 있으며, 예를 들어 용기는 피하 주사 바늘로 뚫을 수 있는 마개가 있는 바이알일 수 있다. 조성물에서 적어도 하나의 활성제는 본 명세서에 기재된 바와 같은 불활성화된 SARS-CoV-2이다.
본 발명은 이하의 설명에 제시되거나 도면에 예시된 성분의 배열 및 구성의 세부사항에 대한 적용에 제한되지 않는다. 발명은 다른 실시형태가 가능하고 다양한 방식으로 실시되거나 수행될 수 있다. 또한, 본 명세서에서 사용된 어구 및 용어는 설명을 위한 것이고 제한적인 것으로 간주되어서는 안된다. 본 명세서에서 "포괄하는", "포함하는" 또는 "갖는", "함유하는", "관련하는" 및 이들의 변형의 사용은 이후에 나열된 항목 및 그 등가물뿐만 아니라 추가 항목을 포괄하는 것을 의미한다.
본 명세서에서 달리 정의되지 않는 한, 본 개시내용과 관련하여 사용되는 과학 및 기술 용어는 당업계의 통상인에게 일반적으로 이해되는 의미를 가질 것이다. 더욱이, 문맥상 달리 요구되지 않는 한, 단수 용어는 복수를 포함하고 복수 용어는 단수를 포함해야 한다. 본 개시내용의 방법 및 기술은 일반적으로 당업계에 잘-공지된 통상적인 방법에 따라 수행된다. 일반적으로, 본 명세서에 기재된 생화학, 효소학, 분자 및 세포 생물학, 미생물학, 바이러스학, 세포 또는 조직 배양, 유전학 및 단백질과 핵산 화학과 관련하여 사용되는 명명법 및 기술은 당업계에 잘-공지되고 일반적으로 사용되는 것이다. 본 개시내용의 방법 및 기술은 일반적으로 당업계에 잘 공지된 통상적인 방법에 따라, 그리고 달리 지시되지 않는 한 본 명세서 전반에 걸쳐 인용되고 논의되는 다양한 일반적이고 보다 구체적인 참고문헌에 기재된 바와 같이 수행된다.
본 발명은 다음의 실시예에 의해 추가로 예시되며, 이는 어떠한 방식으로도 추가 제한으로 해석되어서는 안된다. 본 출원 전반에 걸쳐 인용된 모든 참고문헌(문헌 참고문헌, 발행된 특허, 공개된 특허 출원 및 동시-계류 중인 특허 출원을 포함함)의 전체 내용은 특히 상기 본 명세서에서 참조된 교시를 위해 이에 의해 명백히 참고로 포함된다. 그러나, 임의의 참고문헌의 인용은 그 참고문헌이 선행 기술임을 인정하려는 의도가 아니다.
실시예
실시예 1. 약물 물질 생성
SARS-CoV-2의 생성을 위해, JEV 공정 플랫폼(Srivastava et al., Vaccine 19 (2001) 4557-4565; US 6,309,650B1)이 기본으로 사용되었으며, WO2017/109223A1(그 전체로 본 명세서에 포함됨)에 개시된 지카 바이러스 정제에 적용된 바와 같은 공정에서의 개선 또한 고려되었다. 간단히 말해서, 비-감염성 SARS-CoV-2 입자 응집체, HCP 및 기타 LMW 불순물은 황산프로타민 침전 또는 벤조나아제 처리에 의해 제거되고, 생성된 제제는 선택적으로 수크로스 구배 원심분리에 의해 추가로 정제된다. 생성 공정의 개요에 대해 도 1 참조.
이탈리아 로마의 국립 전염병 연구소 "Lazzaro Spallanzani" IRCCS에서 식별 및 특성화된 이탈리아로부터 최초의 SARS-CoV-2 단리물(수탁번호: MT066156), 서열번호: 9에 의해 제공된 DNA 서열에 상응하는 이의 RNA 서열은 본 명세서에 개시된 모든 실시예에서 사용되었다. 다른 신종 코로나바이러스 SARS-CoV-2 단리물은 또한 다음 출처로부터 획득될 수 있다:
1. -EVAg (유럽 바이러스 아카이브), 예를 들어 다음의 균주 중 하나:
BetaCoV/France/IDF0372/2020 (Ref-SKU:014V-03890, https://www.european-virus-archive.com/virus/human-2019-ncov-0); 2019-nCoV/Italy-INMI1, (Ref-SKU:008V-03893, 서열번호: 9; https://www.european-virus-archive.com/virus/human-2019-ncov-strain-2019-ncovitaly-inmi1); BetaCoV/Netherlands/01, (Ref-SKU: 010V-03903, https://www.european-virus-archive.com/virus/sars-cov-2-strain-nl2020)
2. -BEI 자원 (생물방어 및 신흥 감염 연구 자원): 예를 들어 단리물 USA-WA1/2020, NIAID, NIH: SARS-관련된 코로나바이러스 2, NR-52281(GenBank 수탁 MN985325).
3. -PHE (영국 공중 보건국): https://www.gov.uk/government/collections/contacts-public-health-england-regions-local-centres-and-emergency: 예를 들어 UK B.1.1.7 (UK_MIG457: EVAg Ref-SKU: 004V-04032; 서열번호: 22) 또는 남아프리카 B.1.531 (SA_P2: EVAg Ref-SKU: 004V-04071; 서열번호: 18) 계통의 단리물
SARS-CoV-2로 세포 축적 및 감염. 본 명세서에 기술된 방법에 사용된 Vero 세포는 카탈로그 번호 88020401 하에서 Health Protection Agency 일반 세포 수집에서 얻은 VERO(WHO) 세포주였으며, 이로부터 마스터 세포 은행이 생성되었다. SARS-CoV-2(2019-nCoV/Italy-INMI1 사용 균주)의 연구 바이러스 종자 은행(rVSB)은 Vero 세포 상에 두 차례의 플라크 정제에 이어서 준비되었고 게놈 서열은 서열화에 의해 체크되었다. SARS-CoV-2의 생산을 위해, Vero 세포를 10% 태아 소 혈청(FBS)을 함유하는 이글의 최소 필수 배지(EMEM)에서 성장시키고 단층을 0.01 세포당 플라크 형성 단위(pfu)의 감염 다중도(moi)에서 SARS-CoV-2로 감염시켰다. 대안적으로, moi는 0.001 내지 1, 바람직하게는 0.005 내지 0.2, 바람직하게는 0.01 세포당 플라크 형성 단위(pfu)일 수 있다. 바이러스 흡착을 허용한 후, 배양물을 PBS로 2-4회 세정하고, 무-혈청 Opti-Pro를 공급하고, 바이러스 역가가 원하는 수준에 도달할 때까지 35℃에서 5% CO2로 인큐베이션했다.
SARS-CoV-2 수확 배양 배지를 3일차에 수확하고 수확물을 표준 원심분리기에서 원심분리하였다. 조 수확물을 0.2mg/mL 황산 프로타민으로 처리하고 여과(0.8/0.4μm)한 다음 RT℃에서 15-30분 동안 인큐베이션했다. 숙주 세포 DNA 및 단백질 감소 뿐만 아니라 농축된 물질에서 비-감염성 바이러스 응집체의 감소는 프로타민 설페이트를 사용한 침전에 의해 달성되었다. 정화된 수확물을 TFF 한외여과에 의해 농축하여 세포 배양 배지 성분을 제거하고 배치 부피를 감소시켰다. 대안적으로, 정용여과된 SARS-CoV-2 물질은 벤조나아제로 처리될 수 있다.
선택적 일차 불활성화. SARS-CoV-2 바이러스는 BSL2에서 처리하기에 안전한 바이러스를 만들기 위해 Vero 세포로부터 바이러스-함유 세포 배양 배지를 제거한 직후 베타-프로피오락톤으로 처리에 의해 불활성화되었다. 그러나, 불활성화는 예를 들어 원심분리 후, 프로타민 설페이트 또는 벤조나아제로 처리하기 전, 동안 또는 후에 또는 수크로스 구배 원심분리 전 또는 후에와 같은 정제 공정에서 임의의 단계에서 가능하다. 불활성화는 화학적 불활성화제 예컨대 포름알데히드(포르말린); 효소; 베타-프로피오락톤; 에탄올; 트리플루오로아세트산; 아세토니트릴; 표백제; 요소; 구아니딘 염산염; 트리-n-부틸 포스페이트; 에틸렌-이민 또는 이의 유도체; 유기 용매, 선택적으로 트윈, 트리톤, 나트륨 데옥시콜레이트 또는 설포베타인; 또는 이의 조합의 사용에 의해 수행될 수 있다. 불활성화를 베타-프로피오락톤을 사용하여 수행하는 것이 특히 바람직하며, 이는 바이러스 표면 단백질 및 이의 면역원성 에피토프를 상대적으로 아끼면서 바이러스 RNA를 우선적으로 표적화한다. 불활성화는 또한 pH 변화(매우 높거나 낮은 pH), 열처리 또는 감마 조사 또는 UV 조사, 특히 UV-C 조사와 같은 조사에 의해 달성될 수 있다. SARS-CoV-2 바이러스는 선택적으로, 예를 들어 베타-프로피오락톤 처리 및 UV-C 조사와 같은 2가지 별도 불활성화 단계에 의해 불활성화될 수 있다.
고도로 내성 모델 바이러스 PPV의 불활성화를 위한 BPL 시작 농도의 평가
본 발명자들의 제안된 SARS-CoV-2 BPL 불활성화 절차를 초기에 지원하기 위해 PPV 바이러스 불활성화 역학의 평가를 위한 예비 연구가 수행되었다. 돼지 파보바이러스(PPV)는 물리-화학적 불활성화에 대한 그 높은 내성 때문에 수성 용액에서 BPL의 불활성화 능력을 평가하기 위한 모델 바이러스로 선택되었다. BPL의 3가지 시작 농도, 300ppm(1/3333), 500ppm(1/2000) 및 700ppm(1/1429)가 평가되었다. 바이러스 용액을 이들 농도에서의 BPL로 스파이킹하고 5±2℃에서 24시간 동안 인큐베이션했다. 0.5, 2, 6, 24h 및 BPL 가수분해 단계 후 역학 샘플을 취하고 잔존하는 감염성을 분석했다. 결과는 표 A에 나타나 있다.
Figure pct00004
* 검출 한계 이하
**500ppm BPL에 대한 검출 한계는 700ppm BPL에 대한 것보다 낮다는 것을 주지한다
불활성화 유효성에 대한 초기 BPL 농도의 명확한 효과는 5±2℃(가수분해 전)에서 24시간 인큐베이션 후 3.3과 5.9 log10 사이의 감소로 관찰되었다. 다음 가수분해 단계는 평균으로 1.7 log10 첨가에 의해 역가를 추가로 감소시키는 반면, 유지 대조군 역가는 전반적인 절차에 걸쳐 일정하게 유지되었다. 이는 고도로 내성인 바이러스 오염의 경우 가수분해 단계가 추가 불활성화 단계로 작용할 수 있음을 나타낸다.
4.84(300ppm), 7.43(500ppm) 및 검출 한계 이하(700ppm)의 전반적인 감소 인자로, 적용된 BPL 처리는 농도 > 300ppm에서 파보바이러스과의 불활성화에 효과적인 것으로 간주되었다.
따라서 본 발명자들은 모든 추가 연구에서 SARS-CoV-2 바이러스 불활성화를 위해 500ppm을 선택하기로 결정했다.
BPL에 의한 SARS-CoV-2 바이러스 불활성화
BPL에 의한 모델 바이러스의 불활성화에 대한 기존 데이터(PPV 불활성화에 대한 상기 섹션 참조)를 기반으로 SARS-CoV-2 바이러스 수확 물질의 불활성화를 위해 500ppm(1/2000)의 BPL 농도가 선택되었다. 용액 내 BPL의 안정성은 고도로 온도 의존성이기 때문에 5±3℃의 인큐베이션 온도와 24시간의 인큐베이션 시간이 선택되어 전반적인 불활성화 전반에 걸쳐 충분한 BPL이 존재하도록 보장했다. 농축된 수확물에 BPL을 첨가하고 혼합한 후, 불활성화 용액은 제어된 조건 하에서 불활성화가 일어나는 신선한 용기로 전송된다. 이 전송은 초기 혼합 동안 잠재적인 사각-지점에 있는 바이러스 입자가 BPL과 접촉하지 않을 가능성을 배제한다.
BPL의 가수분해 동안 불활성화된 바이러스 용액의 pH를 안정화하기 위해, 5±3℃로 사전-냉각된 프로타민 설페이트(PS) 처리되고 농축된 수확물에 25mM HEPES pH 7.4를 보충한다.
불활성화된 후 남아있는 BPL을 줄이기 위해 용액을 37±2℃로 설정된 온도-조절된 인큐베이터에서 2.5시간 ± 0.5시간의 총 시간 동안 32℃ 이상의 온도로 가온한다. 현재 공정 부피 약 1L에 대한 가수분해 단계의 총 시간은 32℃ 이상으로의 가온 및 인큐베이션을 포함하여 5시간 15분 내지 6시간 15분이었다.
가수분해의 완료 후, 불활성화된 바이러스 용액(IVS)을 즉시 온도-조절된 냉장고에서 5±3℃로 냉각하고 현재로는 총 18일이 소요되는 대용량 플라크 검정 및 일련의 계대 검정에 의해 불활성화가 확인될 때까지 그곳에 보관하였다.
불활성화 과정 전반에 걸친 바이러스 입자의 회수는 크기-배제 크로마토그래피에 의해 모니터링되었다.
15mL에서 최대 1000mL까지의 실험실 규모에서의 초기 연구는 BPL 추가 후 2시간 이내에 최대 8 log10 pfu/mL의 바이러스 역가가 검출가능한 수준 아래로 감소한 SARS-CoV-2에 대한 매우 빠른 불활성화 동역학을 나타냈다. 이들 결과는 대략 1L의 최종 불활성화 부피에서 GMP 생성 실행에 대해 확인되었다.
모델 바이러스에 대한 불활성화 데이터와 함께 고려할 때 적용된 BPL 처리는 효율적인 것으로 간주될 수 있고 SARS-CoV-2 농축된 수확 물질의 불활성화에 대한 상당한 안전 한계를 포함한다.
추가의 바람직한 실시형태에서, 불활성화 단계(들)는 표면 항원 완전성, 특히 S 단백질의 완전성을 보존하기 위해 특히 온화하다. 일 실시형태에서, 온화한 불활성화 방법은 용기에서 SARS-CoV-2 입자를 포함하는 액체 조성물을 화학적 바이러스 불활성화제(예컨대 예를 들어, 상기 열거된 임의의 화학적 불활성화제 또는 이의 조합, 바람직하게는 베타-프로피오락톤)와 접촉시키는 것, 난류가 아닌 층류의 조건 하에서 화학적 바이러스 불활성화제 및 SARS-CoV-2 입자를 포함하는 액체 조성물을 혼합하는 것, 및 화학적 바이러스 불활성화제 및 SARS-CoV-2 입자를 포함하는 액체 조성물을 바이러스를 불활성화하기에 충분한 시간 동안 인큐베이션하는 것을 포함한다. 온화한 불활성화 단계는 선택적으로 유연한 생물반응기 백에서 수행된다. 온화한 불활성화 단계는 바람직하게는 불활성화의 기간 동안 5회 이하의 용기 반전을 포함한다. 바람직하게는, 화학적 바이러스 불활성화제 및 SARS-CoV-2 입자를 포함하는 조성물의 혼합은 용기를 인큐베이션의 기간 동안 10rpm 이하에서 10분 이하 동안 요동, 회전, 궤도 진탕 또는 진동시키는 것을 포함한다.
SARS-CoV-2의 정제. 선택적으로, 물질은 ~1% CC700 (CC700) 또는 CC400의 최종 농도에서 Capto™ Core 700 또는 CC400 크로마토그래피 매질을 사용한 배치 흡착(본 명세서에서는 배치 크로마토그래피로도 알려짐)에 의해 즉시 추가 처리되었다. 물질을 자기 교반기를 사용하여 일정한 교반 하에 4℃에서 15분 동안 인큐베이션하였다. 인큐베이션 후, CC700 또는 CC400 고형물을 사용하는 경우 이를 중력에 의해 10분 동안 침전시키고 CaptoCore 입자에 의한 필터의 막힘을 피하기 위해 SARS-CoV-2 물질을 용액의 상단에서 제거한다. 임의의 잔존하는 CaptoCore 입자와 DNA 침전물은 그 다음 0.2μm Mini Kleenpak EKV 필터 캡슐(Pall)을 사용하여 여과에 의해 용액에서 제거했다. 여과된 수확 물질을 성분 둘 모두의 스톡 용액을 사용하여 25mM Tris pH 7.5 및 10% 수크로스(w/w)의 최종 농도로 조정했다. 이를 통해 필요한 경우 <-65℃에서 농축된 수확물을 동결할 수 있다.
생성된 여액은 SARS-CoV-2 물질의 최종 농축 및 폴리싱을 위해 수크로스 밀도 구배 원심분리(본 명세서에서는 배치 원심분리로도 알려짐)에 의해 추가로 처리된다. 농축된 프로타민 설페이트(PS) 또는 벤조나아제 처리된(바람직하게는 PS 처리된다) 수확물은 밀도가 다른 3개의 수크로스 층으로 구성된 용액의 상단 상에 장입되었다. 100mL 병 규모에서의 원심분리를 위한 개별 층의 부피는 표 1에 나타나 있다.
표 1: 수크로스 밀도 원심분리를 위한 부피.
Figure pct00005
수크로스 구배 병은 가장 낮은 수크로스 밀도(10% 수크로스(w/w))를 갖는 SARS-CoV-2 물질로 시작해서 뒤이어 오름 차순의 다른 수크로스 용액을 사용하여 병의 바닥 안으로 용액을 펌핑함에 의해 개별 수크로스 층을 층화하여 준비했다. 기술된 설정은 도 3에 나타나 있다. 준비된 SG 병을 4℃로 사전-냉각된 로터 안으로 이전하고 브레이크/감속 없이 적어도 20시간 동안 4℃에서 최대 ~11,000 RCF로 원심분리했다.
원심분리 후, 수크로스 구배의 일련의 2mL 분획의 수확은 연동 펌프를 사용하여 바닥에서 위로 수행한다. 충분히 높은 순도를 갖는 바이러스-함유 분획을 동정하기 위해 분획을 즉시 SDS-PAGE/은 염색에 의해 시험하였다. 따라서, 동정된 분획을 풀링하고 추가 처리했다. 정제된 SARS-CoV-2는 <-65℃에서 보관하거나 즉시 제형화했다.
어쥬번트로 SARS-CoV-2의 제형화 CpG, 및 선택적으로 명반을 SARS-CoV-2 조성물에 첨가하고/하거나 베드사이드 혼합을 위해 별도의 바이알에 제조했다.
SARS-CoV-2 ELISA 검정. 본 명세서에 기재된 제제에서 불활성화된 SARS-CoV-2 항원 함량(즉, 주요 항원성 단백질로서 S1의 함량)은 ELISA에 의해 결정(정량화)되었다. 본 명세서에 사용된 SARS-CoV-2 ELISA는 SARS-CoV-2 샘플이 첨가된 마이크로타이터 플레이트 상에 고정화된 SARS-CoV-2 스파이크 항체(AM001414; 코팅 항체)로 4-층 면역-효소적 검정이다. 코팅 항체에 대한 항원의 결합 시, 플레이트를 일차 항체(즉, AbFlex®SARS-COV-2 스파이크 항체(rAb)(AM002414))로 추가 처리하였다. 이어서 효소 연결된 접합체 항체(즉, 염소 항-마우스 IgG HRP 접합체)인 2차 항체를 첨가하였다. 플레이트는 임의의 비결합된 단백질 또는 항체를 제거하기 위해 순한 세제 용액(PBS-T)을 사용하여 다양한 단계 사이에 세정되었다. 플레이트는 테트라메틸 벤지딘(TMB) 기질의 첨가에 의하여 전개되었다. 가수분해된 TMB는 샘플에서의 항원 함량의 농도에 직접적으로 비례하는 안정한 유색 접합체를 형성한다. 항원 정량화는 자동화된 플레이트 판독기에서 생성된 표준 곡선을 참조로 사용하여 λ450nm(λ630nm 참조)에서 분광광도계 검출에 의해 수행되었다. 표준은 20 항원 단위(AU)/mL 스파이크 삼량체 작업 용액 순수로 시작하여 제조되어, 다음 표준 농도를 위해 1:2로 추가로 연속으로 희석되었다: 20AU/mL, 10AU/mL, 5AU/mL, 2.5AU/mL, 1.25AU/mL, 0.625AU/mL, 0.3125AU/mL 및 0.1263AU/mL. 각 희석은 플레이트당 이중으로 시험되었다. 공급업체(R&D Systems)에 따르면 스파이크 삼량체 표준의 "항원 단위"는 재조합 인간 ACE-2 His-tag를 사용한 기능적 ELISA에서의 그 결합 능력에 상응한다.
참조 표준 및 항체:
코팅 항체: SARS-CoV-2 스파이크 항체(AM001414)
스파이크 삼량체(S1+S2), His-tag(SARS-CoV-2)(예를 들어, BPS Lot# 200826; Cat#100728)
SARS-CoV-2 QC(예를 들어, RSQC240920AGR)
일차 검출 항체 AbFlex® SARS-CoV-2 스파이크 항체(rAb)(AM002414)
이차 검출 항체 염소 항-마우스 IgG HRP 접합체
코팅 완충액: 탄산염 완충액
ELISA 세정 완충액: PBS + 0.05% 트윈-20(PBS-T).
샘플 희석 완충액: PBS-T + 1% BSA.
생산 공정은 고밀도이고 온전한 스파이크 단백질을 전달했다(도 7 참조). AU당 약 1 내지 1.5 x 107 바이러스 입자로 추정되었다. 베타 프로피오락톤에 의한 불활성화 공정은 빠른 불활성화 동역학을 제공하고 S-단백질의 화학적 변형을 감지할 수 없었다. 핵심 매개변수 및 관련 공정 관련된 불순물은 상용 IXIARO® 생성 공정과 유사했다(표 1b 참조). 발명에 따른 SARS-CoV-2 약물 물질은 SDS-PAGE(은 염색, 환원됨)에 따라 고순도(>95%)이고 SE-HPLC에 따라 응집체가 없었다(단량체 바이러스(>95%)(도 8 참조).
베타-프로피오락톤-불활성화된 SARS-CoV2에 따른 S-단백질의 변형을 특성화하기 위한 추가 확인 연구는 S-단백질의 트립신 소화물의 질량 분광 분석에 의해 수행된다. 중요한 에피토프에서 아미노산의 변형은 최소화된다. S 단백질 내의 수용체 결합 도메인(RBD)의 초기 정렬 및 몇 가지 공지된 (교차)-중화 항체(SARS-CoV 및 SARS-CoV-2)의 hACE2 인터페이스 및 에피토프는 잠재적인 높은 전환을 갖는 이들 에피토프 및 잠재적인 낮은 전환율을 갖는 단지 소수 내에서 아미노산을 나타내지 않았다.
표 1b. SARS-CoV-2 약물 물질 및 IXIARO® 약물 물질의 핵심 매개변수 및 관련 공정 관련된 불순물의 비교.
Figure pct00006
실시예 2. 불활성화된 CoV-2 바이러스의 면역원성 및 보호능과 CpG의 어쥬번트화/용량-절감 효과의 시험관내 및 생체내 평가
면역원성 면역화에 앞서, 10마리 BALB/c 마우스의 실험군을 채혈하고 면역-전 혈청을 제조하였다. 마우스에 명반 또는 명반 및 CpG 1018과 함께 제형화된 불활성화된 SARS-CoV-2의 용량 적정을 피하로 투여했다(표 2 참조). 면역화 후 2개의 상이한 간격(아래 참조)에서 혈액을 수집하고 면역 혈청을 준비하고 최종 채혈 시 비장을 수집했다. 모든 동물 실험은 오스트리아 법(BGB1 Nr. 501/1989)에 따라 수행되었으며 "Magistratsabteilung 58"에 의해 승인을 받았다. 혈청은 ELISA에 의해 총 IgG 및 서브클래스(IgG1/IgG2a)에 대해 평가되었고 PRNT에 의해 중화 항체에 대해 평가되었다. Th1/Th2 반응은 IFN-γ ELISpot 및 세포내 사이토카인 염색(CD4+/CD8+)에 의해 추가로 평가되었다.
- 일정 1: 면역화 0일차/7일차, 중간 채혈 14일차, 최종 채혈 및 비장 수확 28일차
- 일정 2: 면역화 0일차/21일차, 중간 채혈 14일차/28일차, 최종 채혈 및 비장 수확 35일차
표 2. 투여 실험의 설계, 10마리 마우스/군: 3개 투여량 군, 0.1-1mAU의 범위인 더 높은 투여량을 갖는 첫 번째 실험 세트(A), 0.05-0.5mAU의 범위인 더 낮은 투여량을 갖는 두 번째 실험 세트(B). (총 바이러스 단백질/mAU는 대략 2μg/ml로 추정됨).
Figure pct00007
*mAU - 용량 당량당 SE-HPLC 피크 면적(밀리-흡수 단위 x 분으로 기록됨; mAU)
플라크 감소 중화 테스트(PRNT). 12-웰 조직 배양 플레이트의 각 웰에 Vero 세포를 시딩하고 3일 동안 5% CO2와 함께 35℃에서 인큐베이션했다. 각 처리 군으로부터 열-불활성화된 혈청의 풀에서 일련의 희석을 시험한다. 각 혈청 제제는 대략 50-80pfu의 SARS-CoV-2로 35℃에서 5% CO2와 함께 1시간 동안 인큐베이션되었다. 세포 배양 배지를 Vero 세포로부터 흡인하고 SARS-CoV-2/혈청 혼합물을 각 웰에 첨가하였다. 플레이트를 부드럽게 흔든 다음 5% CO2와 함께 35℃에서 2시간 동안 인큐베이션한다. 각 웰에 EMEM 및 영양소를 함유하는 2% 메틸셀룰로오스 용액 1mL를 첨가하고 플레이트를 5% CO2와 함께 35℃에서 4일 동안 추가로 인큐베이션하였다. 그런 다음 세포를 크리스탈 바이올렛/5% 포름알데히드로 1시간 동안 염색하고 탈이온수로 3회 세정했다. 플레이트를 공기 건조시키고 각 웰에서의 플라크 수를 수동으로 계수했다. 대안적으로, 예를 들어, TCID50과 같은 다른 방법을 적용할 수 있다.
표 3. 일정 및 수명 실험의 설계. 표 2와 같지만 이에 부가하여; 2차 면역화 후 2, 6, 10, 14, 18 및 22주에 중간 출혈; 2차 면역화 후 26주에 최종-출혈의 면역화 일정.
Figure pct00008
보호능. 불활성화된 SARS-CoV-2의 보호능은 인간화된 ACE2 단백질을 발현하는 SARS-감수성 형질전환 마우스(Jackson Laboratory)(Tseng, C.-T.K. et al., Severe Acute Respiratory Syndrome Coronavirus Infection of Mice Transgenic for the Human Angiotensin-Converting Enzyme 2 Virus Receptor (2007) J of Virol 81:1162-1173) 또는 SARS-CoV-2 감염에 대해 개발된 NHP 모델을 사용하여 평가된다. 동물의 군은 음성 대조군으로서 어쥬번트 또는 PBS를 갖거나 갖지 않는 다양한 투여량의 불활성화된 SARS-CoV-2로 피하로(s.c.) 면역화된다. 마지막 용량 3주 후에 동물은 SARS-CoV-2로 공격되고 질환 진행 및 생존에 대해 모니터링된다. 부가하여, PRNT 검정에서 백신접종에 의해 유도된 중화 항체 역가를 결정하기 위해 혈청 샘플을 취한다.
표 3A. SARS-CoV-2 ELISA-결정된 투여량을 사용한 실험 4743을 투여하는 설계.
Figure pct00009
실험 4743 프로토콜 암컷 BALB/c 마우스(10마리 마우스/군)를 표 3A에 설명된 바와 같은 용량 및 어쥬번트로 0일차 및 21일차에 2회 피하 주사로(100μL) 면역화시켰다. 실험의 판독값은 전체 IgG 및 서브클래스(IgG1/IgG2a) 및 바이러스 중화(PRNT)였다. 실험 4743에 사용된 백신 제형: 17μg Al3+(명반)/용량 및 10μg/용량 CpG 1018로 PBS에서 제형화된 연구 바이러스 종자 은행(rVSB)에서 생산된 정제된 불활성화된 SARS-CoV-2.
SARS-CoV-2 단백질에 대한 항체 반응. 다양한 용량 및 어쥬번트 제형에 대한 마우스에서의 면역 반응을 총 IgG ELISA로 평가했다(도 4). 플레이트는 스파이크 당단백질 또는 핵단백질(도 4c)의 S1 부분(도 4a) 또는 수용체 결합 도메인(RBD)(도 4b)으로 코팅되었다. 28일 및 35일에 채취한 혈청을 분석했다. 플레이트를 2μg/mL 항원(S1, RBD 및 N 단백질)으로 코팅하고 마우스 혈청을 4-배 희석에서 1:50의 시작 희석으로 시험했다. 검출을 위해 2차 모노클로날 항체(HRP-접합된 염소 항-마우스 IgG)를 사용하고 ABTS로 전개하고 흡광도 405nm에서 판독했다. 웰은 각 단계 사이에 PBS-T로 세정되었다. 블랭크 3-배로 설정된 컷-오프를 사용하여 평가변수 역가를 결정했다.
IgG 서브클래스 면역 반응. 플레이트를 스파이크 당단백질의 S1 부분(도 4a)으로 코팅하고 35일차에 취한 혈청을 분석했다. HRP와 접합된 서브클래스 특이적 2차 항체(IgG1 및 IgG2a)를 검출에 사용했다. 상이한 IgG 서브클래스(IgG1 및 IgG2a)의 양 결정을 위한 표준 곡선(4-매개변수 회귀)으로서, 상이한 서브클래스를 갖는 모노클로날 항체가 사용되었다(IgG1 mAb 클론 43 및 IgG2a mAb 클론 CR3022). 결합된 HRP-접합된 2차 mAb는 ABTS로 전개되었고 흡광도 405nm에서 판독되었다. 웰은 각 단계 사이에 PBS-T로 세정되었다. 상대적인 IgG 서브클래스 농도는 도 5a에 도시되고 IgG2a/IgG1의 비율은 도 5b에 도시된다.
실험 4743으로부터 관찰 명반 및 CpG 1018로 제형화된 불활성화된 SARS-CoV-2는 S1 단백질, 수용체 결합 도메인(RBD) 및 뉴클레오캡시드 단백질(N)에 대한 항체를 측정하는 ELISA에 의해 검출된 SARS-CoV-2에 대한 마우스에서 항체를 유도했다(도 4a-c). 면역원성의 증가는 28일차와 35일차에서의 출혈 사이에 관찰되었다. 면역원성의 유의한 증가, 즉 용량-절감 효과는 CpG 1018의 존재에서 S1 및 RBD에 대해 나타났다. 가장 낮은 용량(0.3AU)을 받은 군에서, 핵단백질, S1 및 RBD ELISA 역가에 대해 위약보다 유의하게 높지 않은 더 작은 증가가 관찰되었다.
명반-어쥬번트된 불활성화된 SARS-CoV-2에 CpG 1018의 추가는 S1 ELISA에 의한 IgG 서브클래스의 정량화에 의해 입증된 바와 같이 Th2(IgG1)에 비해 Th1(IgG2a)에 대한 실질적인 면역 반응 이동을 촉진했다. 측정된 IgG2a 및 IgG1의 양 및 처리군에서 IgG2a:IgG1의 비율은 각각 도 5a 및 5b에 도시되어 있다. CpG 1018의 존재에서 Th1(IgG2a)에 대한 면역 반응에서 상당한 전이가 관찰되었다. CpG 1018의 존재에서 IgG1보다 더 강한 IgG2a의 유도가 관찰되었다. 명반군에서만 IgG2a보다 더 강한 IgG1의 유도가 관찰된다.
마지막으로, 가장 높은 SARS-CoV-2 용량으로 면역된 군에서 명반/CpG의 존재에서 중화 반응은 SARS-CoV-2에 대해 양성인 회복기 공여자로부터 혈장의 범위 내에 있었다(NIBSC 20/162; 3개 풀링된 공여자)(도 6 참조).
추가 면역화 실험이 연구와 GMP 물질 사이의 가교로서 저용량(3, 1.2 및 0.3 AU)으로 GMP 물질을 사용하는 마우스에서 수행될뿐만 아니라 인간 용량(540, 10 및 3 AU)으로 마우스에서 GMP 물질의 분석이 수행된다.
추가로, 면역화된 비-인간 영장류(NHP)에서 공격 연구가 수행되고(도 9 참조) 발명의 SARS-CoV-2 백신 후보로 백신접종된 인간 대상로부터의 혈청을 사용하여 햄스터에서 수동 전이 연구가 수행된다(표 1c 참조).
표 1c. 햄스터에서 발명의 SARS-CoV-2 백신 후보의 수동 전이 연구.
Figure pct00010
실시예 3. 질환 및 면역병리의 항체의존 면역증강(ADE)에 대한 SARS-CoV-2 백신의 시험
메커니즘은 잘 이해되지 않지만 이전 코로나바이러스 감염 또는 백신접종에 대한 반응으로 생성된 항체는 후속 코로나바이러스 감염 동안 1) 면역병리 및/또는 2) 질환의 항체의존 면역증강에 대한 위험을 증가시킬 수 있다. 이와 같이 SARS-CoV-2에 대한 항체의 임의의 자극은 가상의 위험을 나타낸다. 이와 관련하여 현재 백신의 안전성을 보장하기 위해 여러 가지 접근법이 수행된다.
시험관내 항체의존 면역증강 검증. 불활성화된 SARS-CoV-2 백신접종된 마우스로부터의 면역 혈청을 시험관내에서 면역증강된 질환의 특징에 대해 평가한다. 이러한 검정은 예를 들어 Wang, S.-F., et al. 2014 (Antibody-dependent SARS coronavirus infection is mediated by antibodies against spike proteins (2014) BBRC 451:208-214)에 의해 기술되어 있다. 간단히 말해서, 감수성 세포 유형 또는 세포주는 면역 혈청과 함께 인큐베이션된 후 SARS-CoV-2로 감염된다. 세포는 세포변형 효과 및 염증 마커의 생성에 대해 평가된다.
면역 병리학의 마우스 모델. 공격 시 백신-증강된 면역병리의 위험은 Tseng C.T. et al. (Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus (2012) PLoS ONE 7(4):e35421)에 의해 기술된 바와 같이 BALB/c 마우스 모델에서 평가된다. 간단히 말해서, 마우스는 본 명세서에 기재된 바와 같이 제형화된 불활성화된 SARS-CoV-2로 2-주 간격으로 2회 면역화시킨 후 SARS-CoV-2로 공격한다. SARS-CoV-2 역가와 폐의 면역 세포 침윤을 시험한다.
ADE의 비-인간 영장류 모델. 비-인간 영장류에서 ADE 발달의 위험은 Luo F, et al. (Evaluation of Antibody-Dependent Enhancement of SARS-CoV Infection in Rhesus Macaques Immunized with an Inactivated SARS-CoV Vaccine (2018) Virologica Sinica 33:201-204)에 의해 기술된 바와 같이 평가된다. 간단히 말해서, NHP는 불활성화된 SARS-CoV-2로 면역화되고 이어서 SARS-CoV-2 공격 및 증상과 질환 병리의 평가가 뒤따른다.
실시예 4. 임상 1상 연구
1상 시험을 위한 불활성화된 SARS-CoV-2의 제형 1상 시험의 목적은 면역원성과 함께 백신의 안전성을 평가하고 건강한 인간 대상체에서 최적의 용량과 어쥬번트를 결정하는 것이다. 이와 같이 임상 1상에서 3가지 항원 용량이 시험되었다: 높음, 중간 및 낮음으로, 대략 3-배의 각 용량 사이의 거리를 가지고 스팬이 높은 용량과 낮은 용량 사이의 약 10-배 차이를 커버하도록 선택된다(예를 들어 0.5, 2 및 5μg/용량 또는 3, 10 및 40AU/용량). 18세에서 55세 사이의 용량군당 50명 대상체로, 약 150명 건강한 지원자가 등록되었다(153명)(도 10 참조). 용량 범위는 CpG의 임의의 잠재적인 용량-절감 효과를 나타내기 위해 부분적으로 선택된다. 문헌에서 이전에 보고된 바와 같이, CpG의 존재에서 필요한 항원에서의 2-3배 감소가 예상될 수 있다. 3가지 투여량이 항원 없이 그리고 1mg CpG 플러스 0.5mg 명반(Al3+)으로 시험된다. 현재 1차 면역화가 완료되었고, 2차 면역화가 진행중이다. 센티넬 대상체(N=15)로부터 초기 안전성 데이터의 이용가능성에 이어서 2개 DSMB 회의가 첫 번째 회의로 수행되었으며, 확인된 안전성 문제가 없으므로 맹검 무작위 연구 단계가 개시되었다. 첫 번째 백신접종(N=153)의 완료 시 중간 안전성 검토를 위한 2차 DSMB에서, 확인된 안전성 문제는 없었다. 3상에 대한 용량 결정을 위한 기초로 2021년 4월까지 첫 번째 데이터 판독(36일차 안전성 및 면역원성)이 예상되었다(실시예 5 참조).
본 명세서에서 정제된 SARS-CoV-2 바이러스는 SDS-PAGE, SE-HPLC 및/또는 SARS-CoV-2 ELISA에 의해 평가된 바와 같이 >90%의 고순도를 갖는다(예를 들어, 도 8). 더욱이 예비 연구에 따르면 바이러스가 통과하는 동안 유전적 이질성의 발현률이 낮고 특정 개별 돌연변이가 두드러지지 않는 것으로 나타났다(데이터는 나타내지 않음).
용량 범위에 도달하기 위해, SARS-CoV-2 바이러스를 JEV와 비교하여, 특히 용량 등가물당 SE-HPLC 피크 면적(밀리-흡수 단위 x 분으로 기록됨; mAU), 용량당 불활성화된 바이러스 입자의 총량 및 용량당 총 바이러스 표면 등가물을 평가한다(표 4 참조). 이 평가는 S(스파이크; SARS-CoV-2)와 E(엔벨로프; JEV) 단백질 사이의 유사한 표면 항원 밀도의 가정을 기반으로 하였다. 총 단백질은 μBCA 검정에 의해 결정되었다(표 4). 검정은 다양했지만 mL당 1mAU에서 ~2μg 총 단백질에 해당하는 것으로 관찰되었다. 실시예 1에 요약된 바와 같이 최적화된 SARS-CoV-2 S-단백질 ELISA를 사용한 또 다른 결정도 수행되었다.
표 4. JEV 및 SARS-CoV-2 정량화 매개변수 및 1상 임상 시험을 위한 낮은, 중간 및 높은 SARS-CoV-2 투여량 군에서 총 단백질의 근사치의 비교.
Figure pct00011
SARS-CoV-2 바이러스 입자(~92nm 직경)는 플라비바이러스 입자(~40nm)보다 훨씬 더 크며, 이는 입자당 대략 5-배 더 큰 바이러스 표면적에 해당하므로 동등하게 더 높은 항원 함량이 예상된다. 더욱이, JEV(IXIARO), TBE(Encepur) 및 HepA(VAQTA)를 포함한 다른 불활성화된 바이러스 백신 제제는 낮은 μg에서 ng 단백질 범위의 항원 용량을 보고했다. 이들 바이러스는 모두 포르말린 불활성화되어 있기 때문에, 본 발명의 BPL-불활성화된 SARS-CoV-2 바이러스는 더 잘 보존된 표면 항원 단백질, 즉 보다 우수한 품질의 항원을 가지고 더 낮은 총 단백질 용량을 요한다.
클리닉에 진입하기 위해 추가 항원 결정 검정(실시예 1에 기술된 SARS-CoV-2 ELISA 검정)이 전개되었고 1상 시험에 진입하기 위한 백신 제형의 용량이 이 검정을 사용하여 결정되었다. 1상 치료 군은 표 5에 제시되어 있다.
1상 시험을 위한 SARS-CoV-2 백신의 제형(0.5mL/용량):
-항원(불활성화된 SARS-CoV-2) 표적 용량(일 용량 = 0.5mL):
낮음: 약 3AU/0.5mL*
중간: 약 10AU/0.5mL)*
높음: 약 40AU/0.5mL*
*실시예 1에 기재된 바와 같이 SARS-CoV-2 ELISA 검정에 의해 결정된 용량
- 수산화알루미늄(Al 3+ ): 0.5mg/용량 (1mg/mL)
-CpG 1018: 1mg/용량(2mg/mL)
-재조합 인간 혈청 알부민(rHSA): ~25μg/용량 (~50μg/mL)
-완충액: 인산완충식염수(PBS)
일부 경우에, 백신접종된 대상체에 감염성 용량의 살아있는 SARS-CoV-2 바이러스(아시아 및/또는 유럽 계통)로 공격한다.
표 5. 불활성화된 SARS-CoV-2 백신의 1상 시험을 위한 처리군(상기에 제시된 낮은, 중간 및 높은 용량). 2가지 용량이 투여된다(0일차 및 21일차).
Figure pct00012
실시예 5. 임상 3상 연구
중화 항체는 강력한 임상 매개변수로 부상(emerging)하고 있다.
ㆍ 중화 항체가 COVID-19에 대한 보호와 연관이 있음을 시사하는 부상하는 임상 증거
ㆍ 중등도 내지 중증 COVID-19 질환으로부터의 보호는 SARS-CoV-2에 대한 중화 항체 수준의 출현과 일치하는 것으로 나타났다(Pfizer/Biontech의 mRNA 백신을 사용한 현장 효능 연구).
ㆍ 단클론 항체(밤라니비맙)는 국립보건원의 국립 알레르기 및 전염병 연구소(NIAID)에서 수행한 3상 시험에서 대상체들을 COVID-19 증상을 전개하는 것을 80%까지 예방하는 것으로 나타났다.
ㆍ 햄스터 또는 비-인간 영장류에서 단클론 항체 투여는 이들 동물 모델에서 COVID-19에 의해 유발된 질환으로부터 보호하는 것으로 나타났다.
ㆍ 회복기 붉은털원숭이(Macaca mulatta)에서 정제된 IgG의 입양 전달은 용량-의존적 방식으로 SARS-CoV-2로의 공격에 대해 순수한 수용체 원숭이를 보호하는 것으로 나타났다.
3상 비-열등성 연구에 대한 계획( 도 11 )
ㆍ 발명의 불활성화된 SARS-CoV-2 백신***의 면역원성을 [활성 비교자, 예를 들어 결정되어 지는 AZD1222 또는 BNT162b2]에 비교하기 위한 무작위, 관찰자-맹검, 대조, 비-열등성 연구.
ㆍ 투여량: 1개월 이내에 2번 i.m. 백신접종; 1/2 상을 기준으로 선택된 용량 수준
ㆍ 연구 모집단: 18세 이상 연령의 4000명* 건강한 지원자로, 발명의 불활성화된 SARS-CoV-2 백신 또는 초기 허가를 위한 안전성 데이터베이스를 확립하기 위한 [활성 비교자]를 투여하기 위해 3:1 무작위화됨
ㆍ 스크리닝에서 SARS-CoV-2에 대해 혈청-음성을 테스트한 대략 600명 참가자**의 면역원성 하위-집합; 발명의 불활성화된 SARS-CoV-2 백신 또는 [활성 비교자]에 1:1 분배됨
ㆍ 일차 평가변수:
o SARS-CoV-2-특이적 중화항체의 GMT 또는 혈청전환율에 의해 결정된 바와 같이, 2-용량 면역화 일정의 완료 4주 후 면역 반응의 비-열등성
* 발명의 불활성화된 SARS-CoV-2 백신으로 백신접종된 3000명 대상체의 샘플 크기는 이 연구에서 94%의 확률로 적어도 1개의 희귀 이벤트(발생률 1/1000)의 검출을 허용할 것이다.
** 1/2상 면역원성 결과의 이용가능성에 의해 확인되는 샘플 크기
*** 도 12 참조. 1상의 임상 연구 물질에 필적하는 임상 상 시험 물질(실시예 1 참조)
실시예 6. 중화 검정으로 백신접종된 유기체의 혈청 시험
백신접종된 마우스, 햄스터, 비-인간 영장류 또는 인간의 혈청은, 예를 들어 "Szurgot, I., Hanke, L., Sheward, D.J. et al. DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice. Sci Rep 11, 3125 (2021). https://doi.org/10.1038/s41598-021-82498-5"에 기술된 바와 같은 중화 검정에서 시험될 수 있다.
시험에서 판독된 내용은 백신접종된 대상체의 혈청이 새로운 변이체를 얼마나 잘 중화할 수 있는지를 제공하고 따라서 백신의 설계를 안내한다.
실시예 7. 불활성화된 SARS-CoV-2의 탠덤 질량 분석법(LC-MSMS) 분석을 사용한 액체 크로마토그래피
방법론:
BPL-불활성화된 SARS-CoV-2 입자의 2개 샘플을 SDS-폴리아크릴아미드 겔 전기영동을 사용하여 분리하고 은 염색에 의해 밴드를 시각화했다. 밴드를 절단하고 트립신으로 겔-내 단리를 수행하고 생성된 펩티드를 고-해상도 정밀 질량 분석기에 연결된 나노-액체 크로마토그래피로 분석했다. 펩티드는 MaxQuant 소프트웨어 패키지와 SARS-CoV-2 및 클로로세부스 사바에우스에 대한 UniProt 참조 데이터베이스를 사용하여 원시 스펙트럼으로부터 식별되었다. 변형을 고려하기 위해 데이터는 특별히 β-프로피오락톤 변형에 대해 재-검색되었고 획득된 결과는 제2 독립적인 검색 알고리즘(Sequest in Proteome Discoverer suite)으로 확인되었다. 부가적으로, 데이터는 추가의 알려지지 않은 MS-감지가능한 변형을 고려하기 위해 FragPipe 패키지로 검색되었다.
결과:
단백질 식별:
밴드는 명확히 3가지 주요 바이러스 단백질(스파이크-단백질, 막-단백질, 핵단백질)뿐만 아니라 숙주 시스템으로부터 배경 단백질에 기인할 수 있었다(도 10 참조). SARS-CoV-2 ORF9b와 레플리카제 다단백질의 흔적도 감지할 수 있었지만, 이들 단백질은 가능하기로는 그 크기에 기인하여 겔에서 잘 분해되지 않았다. 겔 상의 분리 패턴은 숙주 단백질 밴드(밴드 2.3), 약간 다른 S-단백질 패턴(밴드 2.10-2.13) 및 샘플 중 하나에서 혈청 알부민의 예상된 강한 밴드(샘플 2)를 제외하고 샘플 둘 모두에 대해 매우 유사했다. 부가적으로, 인간 기원의 다수의 전형적인 실험실 오염물질(예를 들어, 케라틴)이 샘플 둘 모두의 배경에서 검출되었다. 스파이크-단백질의 처리(전체 길이에서 S1, S2 및 S2'까지)는 적용된 방법론으로 해결하기 어렵지만 샘플 둘 모두에서 밴드 9-13의 패턴에 의해 표시될 가능성이 높다.
변형 분석:
Uittenbogaard et al. (Reactions of β-Propiolactone with Nucleobase Analogues, Nucleosides, and Peptides, Protein Structure and Folding| Volume 286, ISSUE 42, P36198-36214, October 21, 2011)에 의한 공보에 기반하여, 시스테인, 메티오닌 및 히스티딘 상에서 β-프로피오락톤(BPL) 변형을 찾을 것으로 예상되었다. Uittenbogaard et al.은 변형의 유형, 예를 들어, 아실화, 알킬화와 함께 베타-프로피오락톤에 의해 변형 대상이 되는 아미노산을 연구했다. 그들은 BPL이 실제 pH에 따라 최대 9개의 다른 아미노산(C,H,M,D,E,Y,K,E,S)과 반응할 수 있음을 보여주었다. 그들의 연구에서 시스테인(>95%), 히스티딘(15-25%) 및 메티오닌(36%) 잔기에 대해 관련 pH 범위 7에서 9 내에서 더 높은 전환이 관찰되었다. 아스파르트산, 글루탐산 및 티로신에 대한 전환율은 대략 3-15% 범위에서 훨씬 낮았다. 시스틴 잔기에서 이황화기는 반응하지 않는 것으로 나타났다.
BPL-불활성화된 SARS-CoV-2 입자에서, BPL 변형이 감지될 수 있었지만(주로 +72 Da의 형태로) 존재비가 낮다. SARS-CoV-2 단백질에 대해 2894(샘플 1) 및 3086(샘플 2) 식별된 스펙트럼 중에서 각각 단지 73 및 110이 BPL 변형을 수행했으며, 이는 2.5 내지 3.65%로 해석된다(표 6 참조). 이는 또한 FragPipe를 사용한 공개 변형 검색에 의해서 확인되었는데, 이는 BPL-변형과 일치하는 질량 차이로 인해 유사한 낮은 분율의 스펙트럼에 기인했다.
표 6. 식별된 SARS-CoV-2 펩티드 스펙트럼의 수
Figure pct00013
SARS-CoV-2 단백질에 대해 보고된 모든 BPL-변형된 펩티드의 스펙트럼을 수동으로 검사했으며 그 중 샘플 1과 2에 대해 각각 6 내지 8개 부위가 확인되었다(표 6 참조). 이들 검증된 모든 부위에 대해 비변형된 펩티드도 확인되었으며 이는 BPL을 사용한 변형이 100%에 결코 도달하지 않았음을 시사한다. 본 발명자들은 각 밴드에 대한 단백질 풍부도로 정규화된 동일한 변형 부위에 대한 변형된 펩티드 대 비변형된 펩티드의 비율로 특정 부위의 변형(소위 부위 점유)의 정도를 추정했다. 본 발명자들은 그런 다음 부위 변형의 정도를 보수적으로 측정하기 위해 각 부위에 대해 최대 점유를 선택했다. 표 7에서 볼 수 있듯이, 점유는 식별된 스펙트럼의 총 수와 일치하여 식별된 부위에 대해 일반적으로 다소 낮았다. 유일한 예외인 핵단백질의 M234는 주의 깊게 해석되어야 하는데, 그 이유는 특정 펩티드 서열이 다른 부위에 비해 이 특정 펩티드에 대한 추정을 덜 정확하고 신뢰할 수 없게 만드는 문제가 있는 특징을 갖고 있기 때문이다.
표 7. 식별된 BPL-변형 부위 및 그의 점유
Figure pct00014
n.q. = 정량화되지 않음; n.d. = 검출되지 않음
*누락된 절단 및 산화에 기인한 정량화 불확실
예상된 변형과는 별도로, FragPipe 검색은 스펙트럼의 약 10%에서 발생하는 2가지 다른 변형(가장 가능하기로는 아세트알데히드 및 아세틸화)을 밝혔다. 이들 변형은 오염 단백질에서도 발생하기 때문에 가장 가능하기로는 겔 염색 및 샘플 준비 동안 도입된 인공물을 나타낸다.
요약:
상기에 기술된 결과를 기반으로 이들 샘플의 주요 성분은 SARS-CoV2 단백질에 해당한다고 결론지었다. BPL 변형은 검출가능했지만 낮은 것으로, 즉 전체 SARS-CoV-2 프로테옴 수준(즉, 식별된 모든 SARS-CoV-2 단백질)에서 약 3%인 것으로 나타났다. S-단백질의 5개 아미노산만이 변형된 것으로 밝혀졌고 이는 분석된 S-단백질의 소수에 대해서만 검출되었다(예를 들어, H207 아미노산에서 스파이크-단백질의 경우 약 16%, 즉 H207에서 변형을 가질 가능성은 약 16%였다). 두 샘플은 일부 배경 단백질에 관해 그리고 그의 변형의 정도에서 단지 약간 다를 뿐 샘플 1은 BPL-변형의 수준이 약간 더 낮게 나타난다. 스파이크 단백질의 아미노산 중 약 30 내지 40%만이 시험될 수 있었음을 주지한다.
결론:
이 데이터는 발명의 순한 불활성화 접근법이 S-단백질 내의 변형을 최소화하고 따라서 S-단백질의 천연 표면이 크게 보존된다는 견해를 뒷받침한다. 이에 비해, 독감 샘플의 BPL 불활성화에 의한 변형의 결정은 더 빈번했으며, 즉 일 샘플 독감 백신(NIBRG-121xp)의 경우 HA 상의 83개 부위와 NA 상의 43개 부위, 및 다른 샘플(NYMC-X181A)의 경우 HA 상의 99개 부위와 NA 상의 39개 부위가 변형되었으며, 여기서 HA 및 NA는 2가지 주요 막 당단백질, 즉 독감에 대한 일차 면역원이다(She Yi-Min et al., Surface modifications of influenza proteins upon virus inactivation by beta-propiolactone; Proteomics 2013, 13, 3537-3547, DOI 10.1002/pmic.201300096). 따라서 인플루엔자 바이러스의 BPL 불활성화는 막 융합에 영향을 미치는 일부를 포함하여 수많은 단백질 변형을 유발할 수 있다.
실시예 8. 비-인간 영장류에서 면역원성, 중화 항체 반응 및 보호 효과
이 연구에서, 비-인간 영장류(사이노몰구스 원숭이)를 상기 기술된 바와 같이 중간 용량 또는 높은 용량의 불활성화된 SARS-CoV-2-백신으로 면역화시켰다(예를 들어 실시예 4 참조). 면역원성, 중화 항체 반응 및 보호 효과를 그 다음 결정하였다(예를 들어, 실시예 2 및 6 참조).
사이노몰구스 원숭이의 총 IgG 면역 반응
2가지 용량(중간 및 높음)에 대한 사이노몰구스 원숭이의 면역 반응을 총 IgG ELISA로 평가했다. 플레이트를 T4 삼량체화 도메인(A)이 있는 스파이크 단백질의 가용성 엑토도메인 또는 스파이크 당단백질 또는 핵단백질(C)의 수용체 결합 도메인(RBD)(B)으로 코팅했다. 기준선(-1일차) 및 0, 14, 21 28, 35 및 47/49일차에 취한 혈청을 분석했다. 항체 단위(AU)는 AU/mL인 알려진 IgG 농도로 각각의 표준 곡선에 상대적 발광성을 피팅함에 의해 결정되었다.
결과를 도 16에 나타내었다. 불활성화된 SARS-CoV-2-백신은 사이노몰구스 원숭이에서 고도로 면역원성이었고, 면역 반응에서 안정기는 2차 면역화 1주일 후에 이미 도달하였다. 높은 용량과 중간 용량 사이의 면역 반응에는 유의한 차이가 없었다.
사이노몰구스 원숭이에서 중화 항체 반응
불활성화된 SARS-CoV-2-백신으로 면역화한 후 원숭이 면역 혈청에서 기능적 항체의 존재는 혈청 중화 시험에서 평가되었다. 혈청의 일련의 희석액(0, 14, 21, 28, 35 및 47/49일차)을 SARS-CoV-2(SARS-CoV-2 균주 Slovakia/SK-BMC5/2020)와 함께 인큐베이션했으며, 여기서 스파이크 당단백질 특이적 항체가 바이러스에 결합하고, 이에 의해 표적 세포의 표면 상에 ACE2 수용체에 대한 바이러스 부착을 차단하고 세포 내로의 진입을 방지한다. 50% 평가변수 역가(SNT50)는
Figure pct00015
방법으로 결정되었다.
결과를 도 17에 나타내었다. 총 IgG 역가에 관해서는, 2차 면역화 후 1주일(28일차)에 중간 및 높은 용량 둘 모두에 대해 안정기에 도달하였다. 28일차와 35일차에 높은 용량과 중간 용량 간에 차이가 있는 것으로 보이지만 이 차이는 통계적으로 유의하지 않다.
사이노몰구스 원숭이의 바이러스 군체형성
2차 면역화 4주 후, 원숭이는 1×105 PFU SARS-CoV-2(SARS-CoV-2 균주 BetaCoV/France/IDF/0372/2020 SARS-CoV-2)로 비강내(콧구멍당 0.25mL) 및 기관내(4.5mL)로 공격을 받았다. 비인두 및 기관 면봉을 공격 전(35일차) 및 감염 후 1, 2, 3, 4, 7 11 및 15일차에 채취했다. 기관지폐포 세정액(BAL)은 감염 후 3일차에 수집했다. 면봉 및 BAL은 SARS-CoV-2의 존재에 대해 RT-qPCR(게놈 및 서브게놈)로 분석되었다.
결과는 도 18 내지 20에 도시되어 있다. 불활성화된 SARS-CoV-2-백신으로 면역화된 사이노몰구스 원숭이는 SARS-CoV-2로 조합된 비강내 및 기관 공격으로부터 보호되었다. SARS-CoV-2 게놈 RNA는 감염 후 2일차에 면역화된 원숭이에서 일시적으로 비인두 및 기관 면봉에서 매우 낮은 수준으로 검출될 수 있었지만 BAL에서는 전혀 검출되지 않았다. 서브-게놈 SARS-CoV-2 RNA는 비인두 및 기관 면봉이나 BAL에서 전혀 검출가능하지 않았다. 서브-게놈 RNA는 숙주에서 복제된 바이러스의 척도이다. 관찰된 보호의 수준과 관련하여 불활성화된 SARS-CoV-2-백신의 높은 용량과 중간 용량 간에 차이가 검출될 수 없었다.
실시예 9. 랫트에서 불활성화된 SARS-CoV-2 백신의 면역원성
이 연구는 SARS-CoV-2로 인한 COVID-19의 예방을 위한 예방적, 정제된, 불활성화된 SARS-CoV-2 백신에 관한 것이다. SARS-CoV-2는 Vero 세포 상에 전파되고 β-프로피오락톤에 의해 불활성화된다. 이 실시예에서는 불활성화된 SARS-CoV-2 백신의 잠재적 전신 독성 및 국소 내성을 평가하기 위해 암컷 및 수컷 랫트에서 반복 용량 및 국소 내성 독성 연구를 수행했다.
53 항원 단위(AU)/0.5mL의 인간 용량이 알루미늄 염(Alhydrogel) 및 CpG 1018을 사용하여 제형화되었다. 랫트에는 29일의 기간에 걸쳐 2주 간격으로 3회 근육내 주사(2개 부위×0.2mL, 42AU)가 제공되었다. 혈액은 8일차, 15일차 및 22일차 뿐만 아니라 51일차에 경정맥(또는 기타 적합한) 정맥에서 정맥천자에 의해 수집되었다.
연구의 회복기에 배정된 동물로부터 혈청 샘플을 분석하여 백신의 면역원성을 평가하고 시간 경과에 따른 면역 반응을 모니터링했다.
재료 및 방법
장비
Bio-Stack을 갖는 멀티플레이트 세정기 BioTek ELx405 Select CW.
데이터 수집 및 평가를 위해 마이크로플레이트 소프트웨어 Gen5(버전 3.10.06)를 사용하는 멀티플레이트 리더 BioTek Synergy 2.
GraphPad Prism(버전 8.4.3)은 데이터의 플로팅 및 시각화에 사용되었다.
재료 및 시약
Nunc MaxiSorp® 평평한-바닥 96웰 미세역가 플레이트(Thermo Scientific, #439454) 마이크로플레이트, 96웰, PS, F-바닥, 투명(Greiner Bio-One International, #655161) 둘베코의 인산염 완충 식염수(DPBS) 1×(Gibco, #14190-094) 둘베코의 인산염 완충 식염수(DPBS) 10×(Gibco, #14200-067)
소 혈청 알부민(BSA) 분획 V(biomol, #01400.100)
트윈 20(Sigma-Aldrich, #P7949-500ml)
염소 항-랫트 IgG-HRP(Southern Biotech, #3030-05)
ABTS [2,2' 아지노-비스(3-에틸벤조티아졸린-6-설폰산)](Sigma-Aldrich, #A3219-100ml) 나트륨 도데실 설페이트(SDS)(Sigma-Aldrich, #71729)
스파이크 S1 단백질, 78kDa(Aviva, #OPAN06665-100UG)
생물학적 물질
랫트 혈청 샘플을 연구 8, 15, 22 및 51일차에 회복 연구에서의 2개 군으로부터 수집하고(표 8), 테스트 시설에서 냉동하고, 드라이-아이스로 운송하고, 분석할 때까지 -80℃에서 보관했다.
표 8: 실험 설계, 회복군.
Figure pct00016
* 각 투여 상황에서 각 뒷다리에 1회 주사(2×0.2mL)
방법
ELISA
백신-특이적 혈청 항체 반응은 하기에 기술된 바와 같이 ELISA 프로토콜을 사용하여 결정하였다.
ELISA 플레이트(Maxisorp, Nunc)를 PBS에서 100ng 스파이크 S1 단백질로 코팅하고 4℃에서 밤새 인큐베이션하였다. 플레이트를 실온에서 1-2시간 동안 5% BSA, 0.05% 트윈-20, PBS로 차단했다. 플레이트를 PBS/0.1T(0.1% 트윈-20을 갖는 PBS)로 세정했다. 개별 랫트 혈청(플레이트당 5개)을 차단 완충액(5-배 희석)에 희석하고, 96-웰 플레이트에 첨가하고, 실온에서 1시간 동안 인큐베이션함에 의해 이중으로(in duplicates) 시험하였다. 그 다음 플레이트를 PBS/0.1T로 세정하였다. 이차 항체(Goat Anti-Rat IgG-HRP, Southern Biotech)를 차단 완충액에 1:4,000으로 희석하고 96-웰 플레이트에 첨가하고 실온에서 1시간 동안 인큐베이션했다. 플레이트를 PBS/0.1T로 세정하고 ABTS(Sigma-Aldrich)를 기질로 첨가하였다. 30분 동안 인큐베이션 후, 1% SDS의 첨가에 의해 반응을 중단시키고 405nm에서 흡광도를 판독하였다. 반-최대 역가(최고 희석액과 최저 희석액 사이의 평균 흡광도에 상응하는 희석액의 역수)를 결정했다.
샘플 운명
분석의 완료 및 결과의 승인 후, 나머지 샘플은 폐기되었다.
결과
서로 다른 시점에 두 연구 군에서 얻은 혈청 샘플을 ELISA에 의해 분석했다. 각 군의 수컷 및 암컷 랫트에 대한 기하 평균 역가, 시점은 하기 표 9와 나타나 있다.
표 9: 수컷 및 암컷 랫트로 분할된 각 군에 대한 기하 평균 반-최대 역가
Figure pct00017
위약 군(0.9% 염화나트륨)에서의 랫트로부터 혈청은 ELISA로 분석할 때 어떤 시점에서도 반응성을 나타내지 않았다(반-최대 역가 <50)(상기 표 9 참조).
불활성화된 SARS-CoV-2 백신을 백신접종한 군에서의 랫트는 1차 면역화 후 약한 반응을 나타내었지만 측정가능한 반-최대 역가(데이터는 표시되지 않음) 미만이었다. 2차 면역화 1주일 후(22일차) 면역 반응에서의 안정기에 도달했다(상기 표 9, 도 21 및 도 22 참조). 연구 15일차와 22일차에 암컷 랫트에서 약간 더 높은 면역 반응이 관찰되었다(상기 표 9, 도 21 참조). 그러나, 이 차이는 통계적으로 유의하지 않았다.
논의
이 연구에서, 불활성화된 SARS-CoV-2 백신은 모든 랫트에서 고도로 면역원성인 것으로 나타났다. 그러나, 낮은 면역 반응이 단일 면역화에 이어서 관찰되었다. 후속 면역화로 인해 항-스파이크 S1 단백질 항체 역가가 계속 증가했다. 2차 면역화 후에 안정기에 도달했는데, 이는 2회-용량 요법이 적어도 랫트에서 높은 항체 역가에 도달하기에 충분할 수 있음을 시사한다. 3차 면역화는 항체 역가를 더 이상 증가시키지 않았다. 동물에서 정상적인 용량 동역학이 관찰되었다.
결론
이 보고서에 기술된 분석은 불활성화된 SARS-CoV-2 백신이 랫트에서 고도로 면역원성이었으며 랫트에서 강력한 항체 역가를 유도했음을 나타냈다.
실시예 10. 불활성화된 SARS-CoV-2의 탠덤 질량 분석법(LC-MSMS) 분석을 사용한 추가 액체 크로마토그래피
방법론:
단백질의 더 큰 적용범위를 얻기 위하여 실시예 7에 기술된 바와 같이 BPL-불활성화된 SARS-CoV-2 입자의 추가 LC-MSMS 분석을 수행하였다. BPL-불활성화된 SARS-CoV-2 샘플의 5개 분취량을 SDS-PAGE 상에서 분리하고 밴드를 시각화를 위한 은 염색 또는 처리를 위한 쿠마시 염색에 의해 시각화했다. 스파이크 단백질에 해당하는 쿠마시-염색된 밴드(이전 분석 기반)는 트립신 또는 키모트립신을 사용한 겔-내 단리(in-gel digestion) 또는 산 가수분해에 적용되었다. 트립신 단리는 이전 PNGase F(펩티드:N-글리코시다제 F) 단리가 있는 한 번과 없는 한 번인, 두 번 수행하여 글리코실화에 의해 가려진 펩티드를 식별했다.
단리된 펩티드는 본질적으로 실시예 7에 기재된 바와 같이 LC-MSMS에 의해 분석되었다. 특히, 생성된 펩티드는 고-해상도 정밀 질량 분석기에 연결된 나노-액체 크로마토그래피로 분석하였다. 펩티드는 MaxQuant 소프트웨어 패키지와 SARS-CoV-2 및 클로로세부스 사바에우스에 대한 UniProt 참조 데이터베이스를 일반 실험실 오염물질의 데이터베이스와 함께 사용하여 원시 스펙트럼으로부터 식별되었다. 변형을 고려하기 위해 데이터는 특별히 β-프로피오락톤(BPL) 변형에 대해 검색되었고 SARS-CoV-2 스파이크 단백질의 모든 BPL-변형된 펩티드의 스펙트럼이 수동으로 검증되었다. 변형의 정도는 식별된 BPL-변형된 스펙트럼의 백분율로 전체적으로 추정되었고, 별도로 각 펩티드/부위에 대해 변형된 펩티드 대 비변형된 펩티드의 비율로부터 부위 점유를 계산함에 의해 부위-수준에서 추정되었다.
결과:
4가지 단리 방법(즉, (i) 트립신 (ii) 트립신 + PNGase F (iii) 키모트립신 및 (iv) 산 가수분해)의 조합을 사용한 특정 SARS-CoV-2 단백질의 총 적용범위는 다음과 같다:
스파이크(S) 단백질 - 91.5%
막(M) 단백질 - 60.36%
핵단백질(N) - 74.70%
각 단리 방법에 기반한, 불활성화된 SARS-CoV-2 입자에서 BPL-변형된 펩티드의 수는 하기 표 10에 나타나 있다:
표 10: 분석된 모든 밴드에 걸쳐 식별된 SARS-CoV-2 펩티드 스펙트럼의 수
Figure pct00018
실시예 7에 나타낸 바와 같이, 이는 단리 방법에 관계없이 BPL-변형된 펩티드의 백분율이 낮다는 것, 예를 들어 평균 7% 미만, 2 내지 7% 또는 약 2-5%인 것을 확인시켜준다.
상기에 기술된 4가지 단리 방법의 조합을 사용하면 SARS-CoV-2 단백질에서 아미노산 잔기의 더 큰 적용범위가 달성될 수 있다. 따라서, BPL-변형은 하기 표 11에 나타낸 스파이크(S) 및 막(M) 단백질에서의 위치에서 검출되었다. 상기 실시예 7에 기술된 바와 같이 각 부위에서 평균 점유 %도 표 11에 나타나 있다.
표 11. S 단백질에서 식별된 BPL-변형된 부위 및 그의 점유
Figure pct00019
표 11의 데이터로부터, 스파이크(S) 단백질에서 최대 약 16개 잔기가 변형될 수 있고, 막(M) 단백질에서 최대 4개 잔기가 변형될 수 있음을 알 수 있다. 각 부위에서 점유는 예를 들어 20% 미만, 전형적으로 10% 미만으로 낮다. 따라서 불활성화된 SARS-CoV-2 입자는 낮은 정도의 BPL-변형을 나타낸다.
실시예 11. 건강한 대상체에서 COVID-19에 대한 불활성화되고, 어쥬번트된 SARS-CoV-2 바이러스 백신 후보의 안전성, 내약성 및 면역원성을 평가하기 위한 I/II상 무작위, 용량-발견 연구
상기 실시예 4에 제시된 방법론에 기초한 I/II상 임상 연구를 수행하였다. 따라서 제품 조성은 본질적으로 상기 실시예 1 및 4에 기재된 바와 같다. 하기 표 12에 기술된 바와 같이 3가지 용량 수준(낮음, 중간 및 높음)을 연구하였다. 용량 수준(AU/용량 단위)은 실시예 1에 기재된 바와 같이 SARS-CoV-2 ELISA 검정에 의해 결정될 수 있다. 모든 용량 수준에 대해, 어쥬번트는 0.5mg/용량 수산화알루미늄 및 1mg/용량 CpG 1018을 포함하였다.
표 12. 제품 조성 - 0.5mL/용량
Figure pct00020
1DPBS 조성: 200mg/mL KCl(2.68mM), 200mg/mL KH2PO4(1.47mM), 8000mg/mL NaCl(136.9mM), 2160mg/mL Na2HPO4*7H2O(8.06mM); 트리스 완충 식염수: 20mM 트리스, 100mM NaCl, pH 7.5.
연구 설계
이 연구는 3개 용량 군을 갖는 무작위, 용량-증량, 다기관 연구이다. 21일 간격(1일차 및 22일차)으로 각 대상체에게 2가지 용량을 투여했다. 연구 모집단은 18세에서 55세 연령 사이의 건강한 지원자 대략 150명이었다. 연구는 두 파트로 수행된다. 파트 A(1일차부터 36일차까지의 후속 조치 포함) 및 파트 B(37일차부터 208일차까지의 후속 조치 포함). 이 연구는 영국에서 4개 장소인, 버밍엄, 브리스톨, 뉴캐슬, 사우샘프턴에서 수행되었다. 면역화 경로는 근육내(i.m.)였다.
목표
일차 목표:
이 연구의 일차 목표는 18세에서 55세 연령까지의 건강한 성인에서 2회-용량(1일차 및 22일차) 일정의 완료 후 최대 14일까지 불활성화된, 어쥬번트된 SARS-CoV-2 백신 후보의 안전성, 내약성 및 면역원성을 평가하는 것이다.
이차 목표:
18세에서 55세 연령까지의 건강한 성인에서 불활성화된, 어쥬번트된 SARS-CoV-2 백신 후보의 최적 용량 수준을 결정하기 위함.
18세에서 55세 연령까지의 건강한 성인에서 마지막 백신접종 후 최대 6개월까지 불활성화된, 어쥬번트된 SARSCoV-2 백신 후보의 내약성, 안전성 및 면역원성을 평가하기 위함.
표 13. 연구 평가변수
Figure pct00021
결과 - 안전성 및 내약성
일차 평가변수:
각 백신접종 후 7일 이내에 유인된 이상 반응(AE)(국소 및 전신 반응)의 빈도 및 중증도. 유인된 주사 부위 반응은 주사 부위 통증, 가려움증, 압통, 발적 및 부기/경결을 포함한다. 유인된 전신 반응은 발열/체온, 피로, 두통, 메스꺼움/구토, 근육통을 포함한다.
전반적으로, 참가자의 81.7%가 백신접종 후 7일 이내에 적어도 하나의 유인된 반응을 보고했다.
> 참가자의 47.7%(1차 백신접종 후) 및 51.3%(2차 백신접종 후)가 유인된 주사 부위 반응을 경험했다.
> 참가자의 52.3%(1차 백신접종 후) 및 52.0%(2차 백신접종 후)가 유인된 전신 반응을 경험했다.
+ 심각한(3등급) 유인된 이상 반응을 경험한 2명의 참가자가 보고한 3가지 반응을 제외하고 용량 군에 걸쳐 모든 유인된 AE는 경증 또는 중등도로 평가되었다(1명의 대상체: 심한 두통 및 피로, 1명의 대상체: 심한 피로). 두 참가자 모두 높은 용량 군에 속했다.
+ 대부분의 유인된 AE는 백신접종 후 7일 이내에 해결되었다.
유인된 국지 AE:
+ 모든 용량 수준에 걸쳐 백신접종자의 66.7%가, 낮은 용량에서 68.6%, 중간 용량에서 60.8%, 높은 용량 군에서 70.6%로, 적어도 하나의 유인된 주사 부위 반응을 보고했다(통계학적 유의미한 차이 없음; 전반적인 p-값 0.631).
+ 어느 하나의 백신접종 후 가장 흔한 주사 부위 반응은 용량 군에 걸쳐 58.2%에 영향을 미치는 압통과 용량 군에 걸쳐 주사 부위 통증 41.8%였다.
유인된 전신 AE:
+ 모든 용량 수준에 걸쳐 백신접종자의 69.3%가 적어도 하나의 유인된 전신 반응을 보고했다(군 사이에 통계적으로 유의한 차이 없음, 전반적인 p-값 0.507); 유인된 증상이 있는 백신의 비율은 낮은 용량과 높은 용량 둘 모두에서 72.5% 및 중간 용량 군에서 62.7%였다.
+ 전반적으로 백신접종에 이어서 가장 빈번하게 보고된 유인된 전신 부작용은 두통(46.4%), 피로(39.2%) 및 근육통(32.7%)을 포함했다.
비유인된 AE:
+ 전반적으로 참가자의 39.2%가 비유인된 부작용을 적어도 한번 보고했다. 용량 군 사이에 통계적으로 유의한 차이는 관찰되지 않았다.
+ 모든 비유인된 부작용은 경증 및 중등도였다.
+ 36일차까지 PCR에 의해 2건의 COVID-19 사례가 확인되었다. 1차 백신접종 후 16일에 중간 용량 군의 참가자에서 1건의 경증 사례가 발생했다. 2차 백신접종 4일 후 낮은 용량 군의 참가자의 PCR에 의해 중등도의 COVID-19 사례가 확인되었다.
+ 36일차까지 치료-관련된 것으로 간주된 비유인된 이상 반응의 비율은 낮은 용량 군에서 23.5%, 중간 용량 군에서 13.7%, 높은 용량 군에서 15.7%로 17.6%(27/153)였다.
+ 특별한 관심있는 한 가지 이상 반응가 보고되었다(사례 용어: 동상). 사례는 조사자에 의해 연구 백신접종과 관련이 없는 경미한 것으로 평가되었다.
+ AESI는 프로토콜에 따라 심각한 이상 반응(의학적으로 중요한 상태)로 보고되었다; 다른 심각한 이상 반응는 보고되지 않았다.
결론 - 안전성 및 내약성
+ 백신 후보는 시험된 모든 용량 군에서 일반적으로 안전하고 내약성이 우수했으며 독립적인 데이터 안전 모니터링 위원회에서 식별된 안전성 문제가 없다.
+ 용량 군 간에 통계적으로 유의한 차이가 없었고 반응성의 관점에서 1차 및 2차 백신접종 간에 차이가 없었다.
+ 대부분의 이상 반응(AE)는 경증 또는 중등도였고 2명의 대상체만이 심각한 유인된 AE(두통 및 피로)를 보고했다.
+ 유인된 모든 AE가 신속하게 해결되었다.
+ 36일차까지 비유인된 이상 반응의 17.6%만이 백신과 관련된 것으로 간주되었고 심각한 비유인된 AE는 보고되지 않았다.
+ 심각한 관련된 AE는 없었다.
결과 - 면역원성
MNA50에 의해 측정된 중화 항체
중화 항체는 미세중화 검정(MNA50)에 의해 측정하였다. Vero/E6 세포를 미세역가 플레이트에 100μl/웰로 2.5x105 세포/mL로 시딩하고 37℃에서 밤새 인큐베이션한다. 대상체로부터 혈청 샘플은 56℃에서 30분 동안 열 불활성화되었다. 바이러스 및 혈청 샘플을 포함하는 중화 플레이트를 37℃에서 1-1.5시간 동안 준비한다. 그런 다음 중화된 바이러스를 미세역가 플레이트에서의 Vero/E6 세포로 옮기고 37℃에서 1-1.5시간 동안 인큐베이션한다. 세포를 2xMEM에서 2% CMC에 오버레이하고 37℃에서 22-26시간 동안 인큐베이션한다. 세포를 실온에서 >8시간 동안 10% 포르말린으로 고정한다. 그런 다음 플라크는 SARS-CoV-2 RBD 스파이크 항체로 시각화되며, 이차 항체 및 HRP-폴리머에 의해 검출된다. Pfu/웰이 계수되고 ND50을 계산하는 데 사용된다.
+ 명확한 용량 의존적 반응이 22일차(GMT 46.5; 95%CI: 38.79, 55.66)) 및 36일차(GMT 530.4, 95%CI: 421.49, 667.52) 둘 모두에서 높은 용량 군에서 항체를 중화하기 위한 가장 높은 기하 평균 역가(GMT)로 관찰되었다 - 도 23 참조.
+ 36일차에 높은 용량 군의 GMT가 각각의 다른 용량 군보다 통계적으로 유의하게 더 높았다. 낮은 용량에서 GMT는 161.1(95%CI: 121.35, 213.82)이었고 중간 용량 군에서 222.3(95%CI: 171.84, 287.67)이었다.
+ 36일차에서 항체를 중화시키기 위한 GMT-배수 증가는 낮은 용량에서 5.55(95%CI: 4.18, 7.37), 중간 용량에서 7.22(95%CI: 5.64, 9.25) 및 높은 용량 군에서 17.68(95%CI: 14.04, 22.26)이었다.
+ 2차 연구 백신접종 이전, 22일차에서, 항체를 중화시키기 위한 혈청전환이 있는 참가자의 수(기준선에서 ≥4-배수 증가로 정의됨)는 10.0%(참가자 5/50)였다
+ 36일차에 높은 용량 군에서 참가자의 90.0%(95%CI: 0.78,0.97)가 혈청전환되었으며 이는 낮은 용량(51.0%; 95%CI: 0.37, 0.65) 및 중간 용량 군(73.5%; 95%CI: 0.59, 0.85)에 비해 통계적으로 유의하게 높았다 - 도 24 참조.
IgG ELISA에 의해 측정된 S-단백질 결합 항체
S-단백질-결합 항체는 SARS-CoV-2 스파이크 IgG ELISA에 의해 검출되었다. ELISA 플레이트는 스파이크 단백질(Wuhan)로 코팅된다. 혈청 샘플에서 항-S 단백질 항체는 고정된 스파이크 단백질 항원에 결합하고 2차(퍼옥시다제-접합된) 항-인간 IgG 항체에 의해 검출된다. 결과는 mL당 ELISA 실험실 단위(ELU/mL)로 표시된다. 높은 기존 S 항체 역가에 대해 스크리닝된 상업적으로 이용가능한 인간 혈청을 대조군으로 사용한다.
+ S-단백질 IgG 항체 역가는 중화 역가와 상관관계가 있다(r=0.79, p<0.001) - 도 25 참조.
+ 중화 역가와 유사하게, 명확한 용량 의존적 반응이 22일차(GMT 30.0(95%CI: 26.92, 33.48)) 및 36일차(GMT 2147.9(95%CI: 1705.98, 2704.22)) 둘 모두에서 높은 용량 군에서 항체를 중화하기 위한 가장 높은 GMT로 관찰되었다 - 도 26 참조.
+ 36일차에서, 높은 용량 군의 GMT가 각각의 다른 용량 군보다 통계적으로 유의하게 더 높았다. 낮은 용량에서 GMT는 325.1(95%CI: 245.45, 430.46)이었고 중간 용량 군에서 691.6(95%CI: 494.91, 966,52)이었다.
+ 36일차에서 S-단백질 결합 항체에 대한 GMFR은 낮은 용량에서 12.69(95%CI: 9.54, 16.88), 중간 용량에서 26.16(95%CI: 18.73, 36.53) 및 높은 용량 군에서 85.91(95%CI: 68.24, 108.17)이었다.
+ 36일차에서, 높은 용량 군에서 참가자의 100%(95%CI: 0.93,1.00)가 혈청전환되었고 중간 용량 군에서 89.3%(95%CI: 0.78,0.97)뿐만 아니라 낮은 용량 군에서 84.3%(95% CI: 0.71,0.93)(높은 용량 대비 p=0.053)였다(높은 용량 대비 p=0.017).
세포 반응:
탐색적 평가변수는 S-단백질, 막-단백질 및 뉴클레오캡시드-단백질에 대한 IFN감마 ELISpot 분석에 의해 T-세포 반응을 평가했다. 단리된 PBMC(신선)는 S, M 및 N 단백질로부터 SARS-CoV-2 펩티드로 자극된다. T 세포에 의한 인터페론-감마 생산은 항-IFNγ 항체에 의해 검출되고 표지된 2차 항체로 시각화된다. 따라서 활성화된 T 세포에 의해 인터페론-감마가 방출된 곳에 반점이 생성된다. 무 대조군과 식물혈구응집소(PHA) 대조군이 사용된다. 6개 반점 반응성 컷오프가 사용되었으며, 즉 샘플은 2.10x105 PBMC당 정규화된 반점 계수(무 대조군 계수 차감됨)≥6인 경우 개별 자극 패널(펩티드 풀)에 대해 반응성인 것으로 간주된다.
높은 용량 군의 36일차에 연구 참가자의 76%(34/45)가 전장 S-단백질에 걸쳐 있는 펩티드 풀에 대해 반응성이었고(도 27 참조), M-단백질에 대해 36%(16/45)(도 28 참조) 및 N-단백질에 대해 49%(22/45)(도 29 참조)가 반응성이었다.
결론 - 면역원성
+ 백신 후보는 시험된 모든 용량 군에 걸쳐서 SARS-CoV-2 바이러스 스파이크 단백질에 대한 상당한 수준의 항체를 전개하는 모든 연구 참가자의 90% 초과로 고도로 면역원성이었다.
+ S-단백질 결합 IgG 항체에 대한 혈청전환율(SCR)은 중간 용량에서 89.8%, 높은 용량 군에서 100%였다.
+ 2회 용량 일정의 완료 2주 후 기준선으로부터 기하 평균 배수 상승(GMFR)은 중간 용량에서 26 및 높은 용량 군에서 86이었다.
+ 참고로, IgG 항체 반응은 중화 역가(MNA50)와 고도로 상관관계가 있었다(r=0.79, p<0.001).
+ 백신 후보는 낮은 및 중간 용량 군에 비해 높은 용량 군에서 IgG 및 중화 항체 둘 모두에 대해 통계적으로 유의하게 더 높은 기하 평균 역가(GMT)로 용량 의존적 반응을 유도했다.
+ 높은 용량 군에서, 2-용량 일정의 완료 2주 후에 측정한 중화항체 항체 역가의 GMT는 회복기 혈청의 패널에 대한 수준 이상이었다(GMT 530.4(95% CI: 421.49, 667.52)).
+ 백신 대 회복기 혈청의 GMT 비율≥1인 경우 백신 효능은 다른 백신에 대해 80% 이상으로 보고되었다*).
*)Earle et al. MedRxiv, 2021년 3월, https://doi.org/10.1101/2021.03.17.20200246); Khoury et al. MedRxiv, 2021년 3월, https://doi.org/10.1101/2021.03.09.21252641
+ 백신 후보는 각각 연구 참가자의 75.6%, 35.6% 및 48.9%에서 검출된 S-단백질, M 및 N 단백질에 대한 항원-특이적 IFN-감마 생성 T-세포로 참가자 전반에 걸쳐 광범위한 T-세포 반응을 유도했다.
발명의 부가적인 양태
추가 양태에서, 본 발명은 다음을 제공한다:
A1. 최적으로(예를 들어, S-단백질의 천연 표면이 보존된) 불활성화된 SARS-CoV-2 입자를 포함하는 SARS-CoV-2 백신으로서, 여기서 SARS-CoV-2 입자는 SARS-CoV-2 백신이 투여된 대상체를 적어도 70% 확률로 혈청변환할 수 있는, SARS-CoV-2 백신.
A2. 양태 A1에 있어서, SARS-CoV-2 입자는 SARS-CoV-2 백신이 투여된 대상체를 적어도 80%, 85%, 90%, 또는 95% 확률로 혈청변환할 수 있는, SARS-CoV-2 백신.
A3. 양태 A1 또는 A2에 있어서, SARS-CoV-2 입자는 하기의 핵산 서열 중 어느 하나에 의해 제공되는 DNA 서열에 상응하는 RNA 게놈을 갖는, 백신
ㆍ서열번호: 1(Genbank NC_045512.2 참조), 또는 서열번호: 1과 적어도 85% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 핵산 서열; 또는
ㆍ서열번호: 9(NCBI MT066156 참조), 또는 서열번호: 1과 적어도 85% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 핵산 서열; 또는
ㆍ서열번호: 18(NCBI MW598408 참조). 또는 서열번호: 18과 적어도 85% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 핵산 서열; 또는
ㆍ서열번호: 20(NCBI MW520923 참조). 또는 서열번호: 20과 적어도 85% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 핵산 서열; 또는
ㆍ서열번호: 22(NCBI MW422256 참조). 또는 서열번호: 22와 적어도 85% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 핵산 서열; 또는
ㆍ서열번호: 22(NCBI MW422256 참조). 또는 서열번호: 24와 적어도 85% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 핵산 서열; 또는
ㆍ서열번호: 22(NCBI MW422256 참조). 또는 서열번호: 26과 적어도 85% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 핵산 서열.
A4. 양태 A1-A3 중 어느 하나에 있어서, SARS-CoV-2 입자가 아미노산 서열에 의해 정의된 바와 같은 S 단백질을 갖는, 백신
ㆍ서열번호: 3, 또는 서열번호: 3과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 아미노산 서열; 또는
ㆍ서열번호: 11, 또는 서열번호: 3과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 아미노산 서열; 또는
ㆍ서열번호: 19, 또는 서열번호: 3과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 아미노산 서열; 또는
ㆍ서열번호: 21, 또는 서열번호: 3과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 아미노산 서열; 또는
ㆍ서열번호: 23, 또는 서열번호: 3과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 아미노산 서열; 또는
ㆍ서열번호: 25, 또는 서열번호: 3과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 아미노산 서열; 또는
ㆍ서열번호: 27, 또는 서열번호: 3과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이 아미노산 서열.
A5. 양태 A1-A4 중 어느 하나에 있어서, SARS-CoV-2 입자는 서열번호: 2, 10, 13 또는 16 중 어느 하나, 바람직하게는 서열번호: 10에 의해 제공되는 아미노산 서열, 또는 서열번호: 2, 10, 13 또는 16 중 어느 하나, 바람직하게는 서열번호: 10과 적어도 95% 동일하고 독성 SARS-CoV-2를 패킹할 수 있는 변이체 아미노산 서열로부터 선택된 다단백질을 갖는, SARS-CoV-2 백신.
A6. 양태 A1-A5 중 어느 하나에 있어서, SARS-CoV-2는 화학적 불활성화, 열적 불활성화, pH 불활성화, UV 불활성화 또는 방사선 불활성화에 의해 불활성화된, SARS-CoV-2 백신.
A7. 양태 A6에 있어서, 화학적 불활성화는 플라크 검정에 의해 측정되거나 플라크 검정 플러스 일일에 의해 측정된 바와 같이 SARS-CoV-2를 완전하게 불활성화시키는 데 필요한 것보다 더 오래 동안 SARS-CoV-2 입자를 화학적 불활성화제와 접촉시키는 것을 포함하는, SARS-CoV-2 백신.
A8. 양태 A7에 있어서, 화학적 불활성화는 SARS-CoV-2 입자를 포름알데히드 및/또는 베타-프로피오락톤, 바람직하게는 베타-프로피오락톤과 접촉시키는 것을 포함하는, SARS-CoV-2 백신.
A9. 양태 A8에 있어서, 포름알데히드 및/또는 베타-프로피오락톤 불활성화는 SARS-CoV-2 입자를 2-10일 동안 포름알데히드 및/또는 베타-프로피오락톤과 접촉시키는 것을 포함하는, SARS-CoV-2 백신.
A10. 양태 A6-A9 중 어느 하나에 있어서, 화학적 활성화는 약 4℃ 또는 약 22℃에서 수행되는, SARS-CoV-2 백신.
A11. 양태 A1-A10 중 어느 하나에 있어서, 어쥬번트를 추가로 포함하는, SARS-CoV-2 백신.
A12. 양태 A11에 있어서, 어쥬번트는 CpG, 바람직하게는 CpG 1018이고, 선택적으로 또한 알루미늄 염 어쥬번트를 포함하는, SARS-CoV-2 백신.
A13. 양태 A12에 있어서, 알루미늄 염 어쥬번트는 수산화알루미늄 또는 인산알루미늄 염인, SARS-CoV-2 백신.
A14. 양태 A13에 있어서, 수산화알루미늄은 1.25ppb 미만의 Cu를 포함하는, SARS-CoV-2 백신.
A15. 양태 A12 내지 A14 중 어느 하나에 있어서, 명반:CpG(w/w) 비율은 약 1:10, 약 1:5, 약 1:4, 약 1:3, 약 1:2, 약 1:1, 약 2:1, 약 3:1, 약 4:1, 약 5:1, 약 10:1, 바람직하게는 약 1:3 내지 3:1, 보다 바람직하게는 약 1:2 내지 1:1, 가장 바람직하게는 약 1:2인, SARS-CoV-2 백신.
A16. 양태 A12 내지 A14 중 어느 하나에 있어서, 백신 조성물에서 유리(비결합) CpG의 양은 10% 초과, 20% 초과, 30% 초과, 40% 초과, 50% 초과, 60% 초과, 70% 초과, 80% 초과, 90% 초과, 95% 초과, 바람직하게는 약 70% 내지 95%, 가장 바람직하게는 약 80% 내지 90%인, SARS-CoV-2 백신.
A17. 양태 A1 내지 A16 중 어느 하나에 있어서, 백신 조성물은 적어도 하나의 완충제, 바람직하게는 포스페이트 완충제를 포함하는, SARS-CoV-2 백신.
A18. A11-A13 중 어느 하나에 있어서, 백신은 펩티드 및 데옥시이노신-함유 면역자극 올리고데옥시핵산 분자(I-ODN)를 포함하는 어쥬번트를 포함하거나 추가로 포함하는, SARS-CoV-2 백신.
A19. 양태 A18에 있어서, 펩티드는 서열 KLKL5KLK(서열번호: 5)를 포함하고 I-ODN은 올리고-d(IC)13(서열번호: 6)을 포함하는, SARS-CoV-2 백신.
A20. 양태 A1-A19 중 어느 하나에 있어서, 하나 이상의 약학적으로 허용가능한 부형제를 추가로 포함하는, SARS-CoV-2 백신.
B1. 양태 A1-A15 중 어느 하나의 SARS-CoV-2 백신을 포함하는 키트.
B2. 양태 B1에 있어서, 제2 백신을 추가로 포함하는, 키트.
B3. 양태 B2에 있어서, 제2 백신은 (예를 들어, mRNA 또는 아데노바이러스 벡터와 같은 또 다른 기술의) 또 다른 SARS-CoV-2 바이러스 백신, 인플루엔자 바이러스 백신 또는 치쿤구니야 바이러스 백신인, 키트.
C1. 양태 A1-A15 중 어느 하나의 예방적으로 또는 치료적으로 유효한 양의 SARS-CoV-2 백신의 제1 용량을 이를 필요로 하는 대상체에게 투여하는 것을 포함하는, 방법.
C2. 양태 C1에 있어서, 예방적으로 또는 치료적으로 유효한 양의 SARS-CoV-2 백신의 제2 용량을 투여하는 것을 추가로 포함하는, 방법.
C3. 양태 C1 또는 C2에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 7일에 투여되는, 방법.
C4. 양태 C1 또는 C2에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 14일에 투여되는, 방법.
C5. 양태 C1 또는 C2에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 21일에 투여되는, 방법.
C6. 양태 C1 또는 C2에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 28일에 투여되는, 방법.
C7. 양태 C1-C6 중 어느 하나에 있어서, 투여는 SARS-CoV-2 중화 항체의 생성을 초래하는, 방법.
C8. 양태 C1-C7 중 어느 하나에 있어서, SARS-CoV-2 백신의 예방적 또는 치료적으로 유효한 양은 SE-HPLC에 의해 분석시 약 0.01 내지 25mAU(밀리-흡수 단위 x 분), 바람직하게는 약 0.05 내지 10mAU, 더욱 바람직하게는 약 0.1 내지 5mAU, 가장 바람직하게는 약 0.25 내지 2.5mAU로 정의되는, 방법.
C9. 양태 C1-C7 중 어느 하나에 있어서, SARS-CoV-2 백신의 예방적 또는 치료적으로 유효한 양은 (μ)BCA로 측정시 약 0.05 내지 50μg 총 단백질, 약 0.1 내지 25μg, 약 0.25 내지 12.5μg, 바람직하게는 약 0.5 내지 5μg 총 단백질로 정의되는, 방법.
C10. 양태 C1-C7 중 어느 하나에 있어서, SARS-CoV-2 백신의 예방적 또는 치료적으로 유효한 양은 ELISA에 의해 측정시 약 0.025 내지 25μg S-단백질, 약 0.05 내지 12.5μg, 약 0.125 내지 6.25μg, 바람직하게는 약 0.25 내지 2.5μg S-단백질로 정의되는, 방법.
D1. 하기를 포함하는, SARS-CoV-2 백신을 제조하는 방법
(i) Vero 세포 상에서 SARS-CoV-2를 계대하고, 이에 의해 SARS-CoV-2를 포함하는 배양 배지를 생성하는 단계;
(ii) (i)의 배양 배지를 수확하는 단계;
(iii) (ii)의 수확된 배양 배지를 침전시키고, 이에 의해 SARS-CoV-2 상등액을 생성하는 단계; 및
(iv) (iii)의 SARS-CoV-2 상등액에서 SARS-CoV-2를 최적으로 불활성화하고 이에 의해 불활성화된 SARS-CoV-2를 생성하는 단계.
D2. 양태 D1에 있어서, 단계 (iii) 이전에 (ii)의 배양 배지를 농축하는 것을 추가로 포함하는, 방법.
D3. 양태 D1 또는 D2에 있어서, (iii)의 침전은 (ii)의 배양 배지를 프로타민 설페이트 또는 벤조나아제와 접촉시키는 것을 포함하는, 방법.
D4. 양태 D1-D3 중 어느 하나에 있어서, (v) (iv)의 불활성화된 SARS-CoV-2를 투석하고 이에 의해 투석된 SARS-CoV-2를 생성하는 단계를 추가로 포함하는, 방법.
D5. 양태 D4에 있어서, (v)의 투석된 SARS-CoV-2를 여과하는 (vi)를 추가로 포함하는, 방법.
D6. 양태 D1-D5 중 어느 하나에 있어서, 불활성화는 화학적 불활성화, 열적 불활성화, pH 불활성화, 또는 UV 불활성화에 의한 것인, 방법.
D7. 양태 D6에 있어서, 화학적 불활성화는 SARS-CoV-2 입자를 적어도 4일 동안 화학적 불활성화제와 접촉시키는 것을 포함하는, 방법.
D8. 양태 D6 또는 D7에 있어서, 화학적 불활성화제는 포름알데히드를 포함하는, 방법.
D9. 양태 D6-D8 중 어느 하나에 있어서, 화학적 활성화는 약 4℃ 또는 약 22℃에서 수행되는, 방법.
D10. 양태 D8 또는 D9에 있어서, 포름알데히드를 중화시키는 것을 추가로 포함하는, 방법.
D11. 양태 D10에 있어서, 중화는 메타중아황산나트륨으로 수행되는, 방법.
D12. 양태 D1-D11 중 어느 하나에 있어서, 화학적 불활성화는 바람직하게는 300 내지 700ppm, 더욱 바람직하게는 500ppm의 농도에서 BPL로 수행되고 약 1 내지 48시간, 바람직하게는 20 내지 28시간, 가장 바람직하게는 24시간 ± 2시간(예컨대 또한 ±1시간 또는 ±0.5시간) 동안 2℃ 내지 8℃에서 불활성화되는, 방법.
D13. 양태 D12에 있어서, 화학적 불활성화 후에 35℃ 내지 39℃, 바람직하게는 약 37℃에서 2.5시간 ± 0.5시간 동안 가수분해 단계가 이어지는, 방법.
E1. SARS-CoV-2 감염의 치료 및 예방을 위한 양태 A1-A15 중 어느 하나의 최적으로 불활성화된 SARS-CoV-2 백신의 용도.
E2. 양태 E1에 있어서, 불활성화된 SARS-CoV-2 백신은 이를 필요로 하는 대상체에게 치료적으로 유효한 양의 제1 용량으로 투여되는, 용도.
E3. 양태 E2에 있어서, 불활성화된 SARS-CoV-2 백신은 대상체에게 치료적으로 유효한 양의 제2 용량으로 투여되는, 용도.
E4. 양태 E3에 있어서, 불활성화된 SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 7일에 투여되는, 용도.
E5. 양태 E3에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 14일에 투여되는, 용도.
E6. 양태 E3에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 21일에 투여되는, 용도.
E7. 양태 E3에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 28일에 투여되는, 용도.
E8. 양태 E1-E6 중 어느 하나에 있어서, 투여는 SARS-CoV-2 중화 항체의 생성을 초래하는, 용도.
F1. SARS-CoV-2 감염의 치료 및 예방에 사용하기 위한 약학적 조성물로서, 여기서 상기 약학적 조성물은 양태 A1-A15 중 어느 하나의 최적으로 불활성화된 SARS-CoV-2 백신을 포함하는, 약학적 조성물.
F2. 양태 F1에 있어서, 불활성화된 SARS-CoV-2 백신은 이를 필요로 하는 대상체에게 치료적으로 유효한 양의 제1 용량으로 투여되는, 약학적 조성물.
F3. 양태 F2에 있어서, 불활성화된 SARS-CoV-2 백신은 대상체에게 치료적으로 유효한 양의 제2 용량으로 투여되는, 용도.
F4. 양태 F3에 있어서, 불활성화된 SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 7일에 투여되는, 용도.
F5. 양태 F3에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 14일에 투여되는, 용도.
F6. 양태 F3에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 21일에 투여되는, 용도.
F7. 양태 F3에 있어서, SARS-CoV-2 백신의 제2 용량은 SARS-CoV-2 백신의 제1 용량 후 약 28일에 투여되는, 용도.
F8. 양태 F1-F6 중 어느 하나에 있어서, 투여는 SARS-CoV-2 중화 항체의 생성을 초래하는, 용도.
G1. 유효량의 항원을 포함하는 SARS-CoV-2 백신으로서, 여기서 상기 유효량은 SARS-CoV-2 백신이 투여된 대상체를 적어도 70% 확률로 혈청전환시킬 수 있는, SARS-CoV-2 백신.
G2. 양태 G1에 있어서, 상기 유효량은 SARS-CoV-2 백신이 투여된 대상체를 적어도 80%, 85%, 90%, 또는 95% 확률로 혈청전환시킬 수 있는, SARS-CoV-2 백신.
G3. 양태 G1 또는 G2에 있어서, 상기 유효량은 약 1 내지 100AU/용량, 바람직하게는 약 2 내지 75AU/용량, 바람직하게는 약 3 내지 60AU/용량, 보다 바람직하게는 약 3 내지 55AU/용량, 더욱 바람직하게는 약 3 내지 53AU/용량인, SARS-CoV-2 백신.
G4. 양태 G3에 있어서, 상기 유효량이 ELISA에 의해 결정되고 여기서 항원 단위(AU)는 표준으로서 사용된 스파이크 단백질의 ACE-2 결합 능력에 상응하는, SARS-CoV-2 백신.
H1. SARS-CoV-2 항원 및 톨-유사 수용체 9(TLR9) 작용제를 포함하는 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)에 대한 면역 반응을 자극하기 위한 면역원성 조성물로서, 여기서 SARS-CoV-2 항원은 불활성화된 전체 SARS-CoV-2이고, TLR9 작용제는 비메틸화된 시티딘-포스포-구아노신(CpG) 모티프를 포함하는 10 내지 35 뉴클레오티드 길이의 올리고뉴클레오티드이고, SARS-CoV-2 항원 및 올리고뉴클레오티드는 포유동물 대상체에서 SARS-CoV-2 항원에 대한 면역 반응을 자극하기에 효과적인 양으로 면역원성 조성물에 존재하는, 조성물.
H2. 양태 H1에 있어서, 올리고뉴클레오티드는 서열 5'-AACGTTCGAG-3'(서열번호: 30)을 포함하는, 조성물.
H3. 양태 H1에 있어서, 올리고뉴클레오티드는 5'-TGACTGGAA CGTTCGAGAT GA-3'(서열번호: 4)의 서열을 포함하는, 조성물.
H4. 양태 H1-3 중 어느 하나에 있어서, 올리고뉴클레오티드는 변형된 뉴클레오시드를 포함하고, 선택적으로 변형된 뉴클레오시드는 2'-데옥시-7-데아자구아노신, 2'-데옥시-6-티오구아노신, 아라비노구아노신, 2'-데옥시-2' 치환된-아라비노구아노신, 및 2'-O-치환된-아라비노구아노신으로 구성된 군으로부터 선택되는, 조성물.
H5. 양태 H4에 있어서, 올리고뉴클레오티드는 서열 5'-TCG1AACG1TTCG1-3'(서열번호: 31)을 포함하고 여기서 G1은 2'-데옥시-7-데아자구아노신이고, 선택적으로 올리고뉴클레오티드는 서열 5'-TCG1AACG1TTCG1-X-G1CTTG1CAAG1CT-5'(서열번호: 32)을 포함하고 여기서 G1은 2'-데옥시-7-데아자구아노신이고 X는 글리세롤(5'-서열번호: 31-3'-X-3'-서열번호: 31-5')인, 조성물.
H6. 양태 H1-5 중 어느 하나에 있어서, 올리고뉴클레오티드는 적어도 하나의 포스포로티오에이트 연결을 포함하거나, 또는 모든 뉴클레오티드 연결은 포스포로티오에이트 연결인, 조성물.
H7. 양태 H1-6 중 어느 하나에 있어서, 올리고뉴클레오티드는 단일-가닥의 올리고데옥시뉴클레오티드인, 조성물.
H8. 양태 H1-7 중 어느 하나에 있어서, 0.5ml 용량의 면역원성 조성물은 약 750 내지 약 3000μg의 올리고뉴클레오티드를 포함하거나, 또는 면역원성 조성물은 약 750μg, 약 1000μg, 약 1500μg, 또는 약 3000μg의 올리고뉴클레오티드를 포함하는, 조성물.
H9. 양태 H1-8 중 어느 하나에 있어서, SARS-CoV-2 항원은 포유동물 세포에서 시험관내 증식되는, 조성물.
H10. 양태 H1-9 중 어느 하나에 있어서, SARS-CoV-2는 포르말린 및 자외선 중 하나 또는 둘 모두로의 처리에 의해 불활성화되는, 조성물.
H11. 양태 H1-9 중 어느 하나에 있어서, SARS-CoV-2는 베타-프로피오락톤으로의 치료에 의해 불활성화되는, 조성물.
H12. 양태 H1-11 중 어느 하나에 있어서, SARS-CoV-2는 적어도 2개의 상이한 바이러스 균주, 또는 2개의 상이한 바이러스 클레이드 또는 계통으로부터의 조합을 포함하는, 조성물.
H13. 양태 H1-12 중 어느 하나에 있어서, 0.5ml 용량의 면역원성 조성물은 약 0.025 내지 약 25μg의 SARS-CoV-2 스파이크(S) 단백질, 또는 약 0.25 내지 약 25μg의 S 단백질을 포함하는, 조성물.
H14. 양태 H1-13 중 어느 하나에 있어서, 알루미늄 염 어쥬번트를 추가로 포함하는, 조성물.
H15. 양태 H14에 있어서, 알루미늄 염 어쥬번트는 무정형 알루미늄 하이드록시포스페이트 설페이트, 수산화알루미늄, 인산알루미늄, 및 칼륨 알루미늄 설페이트로 구성된 군 중 하나 이상을 포함하는, 조성물.
H16. 양태 H14에 있어서, 알루미늄 염 어쥬번트는 수산화알루미늄을 포함하는, 조성물.
H17. 양태 H14-16 중 어느 하나에 있어서, 0.5ml 용량의 면역원성 조성물은 약 0.05 내지 약 0.50mg Al3+, 또는 약 0.075 내지 약 0.175mg Al3+, 또는 약 0.25 내지 약 0.50mg Al3+, 또는 약 0.375mg Al3+를 포함하는, 조성물.
H18. 양태 H1-17 중 어느 하나에 있어서, 포유동물 대상체는 인간 대상체인, 조성물.
H19. 다음을 포함하는 키트:
i) 양태 H1-18 중 어느 하나의 면역원성 조성물, 및
ii) 포유동물 대상체에서 SARS-CoV-2 항원에 대한 면역 반응을 자극하기 위한 조성물의 투여에 대한 지침.
H20. 양태 H19에 있어서, iii) 면역원성 조성물의 근육내 주사를 위한 주사기 및 바늘을 추가로 포함하는, 키트.
H21. 포유동물 대상체에서 SARS-CoV-2 항원에 대한 면역 반응을 자극하기 위해 양태 H1-18 중 어느 하나의 면역원성 조성물을 포유동물 대상체에게 투여하는 것을 포함하는, 포유동물 대상체에서 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)에 대한 면역 반응을 자극하는 방법.
H22. 양태 H21에 있어서, 포유동물 대상체는 인간 대상체이고/이거나 면역원성 조성물은 근육내 주사에 의해 투여되는, 방법.
H23. 포유동물 대상체에서 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)에 대한 면역 반응을 자극하기 위한 양태 H1-18 중 어느 하나의 면역원성 조성물의 용도로서, 방법은 대상체에게 유효량의 면역원성 조성물을 투여하는 것을 포함하는, 용도.
H24. 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)로의 감염으로부터 포유동물 대상체를 보호하기 위한 양태 H1-18 중 어느 하나의 면역원성 조성물의 용도로서, 방법은 대상체에게 유효량의 면역원성 조성물을 투여하는 것을 포함하는, 용도.
H25. 포유동물 대상체가 COVID-19 질환에 걸리는 것을 예방하기 위한 양태 H1-18 중 어느 하나의 면역원성 조성물의 용도로서, 방법은 대상체에게 유효량의 면역원성 조성물을 투여하는 것을 포함하는, 용도.
H26. 양태 H23-25 중 어느 하나에 있어서, 포유동물 대상체는 인간 대상체이고/이거나 면역원성 조성물은 근육내 주사에 의해 투여되는, 용도.
일부 특정 실시형태에서, 본 명세서에 기재된 바와 같은 발명의 양태가 제공되며(예를 들어, 상기 양태 A 내지 H 중 하나 이상에서, 또는 첨부된 청구범위에서와 같음), 여기서 다음의 양태 중 하나 이상(X로 표지됨)이 제외됨:
X1. SARS-CoV-2 항원 및 톨-유사 수용체 9(TLR9) 작용제를 포함하는 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)에 대한 면역 반응을 자극하기 위한 면역원성 조성물로서, 여기서 TLR9 작용제는 비메틸화된 시티딘-포스포-구아노신(CpG) 모티프를 포함하는 10 내지 35개 뉴클레오티드 길이의 올리고뉴클레오티드이고, SARS-CoV-2 항원 및 올리고뉴클레오티드는 포유동물 대상체에서 SARS-CoV-2 항원에 대한 면역 반응을 자극하기에 효과적인 양으로 면역원성 조성물에 존재하는, 조성물.
X2. 양태 X1에 있어서, 올리고뉴클레오티드는 서열 5'-AACGTTCGAG-3'(서열번호: 30)을 포함하는, 조성물.
X3. 양태 X1에 있어서, 올리고뉴클레오티드는 5'-TGACTGTGAACGTTCGAGATGA-3'(서열번호: 4)의 서열을 포함하는, 조성물.
X4. 양태 X1에 있어서, 올리고뉴클레오티드는 변형된 뉴클레오시드를 포함하고, 선택적으로 변형된 뉴클레오시드는 2'-데옥시-7-데아자구아노신, 2'-데옥시-6-티오구아노신, 아라비노구아노신, 2'-데옥시-2'치환된-아라비노구아노신, 및 2'-O-치환된-아라비노구아노신으로 구성된 군으로부터 선택되는, 조성물.
X5. 양태 X4에 있어서, 올리고뉴클레오티드는 서열 5'-TCG1AACG1TTCG1-3'(서열번호: 31)을 포함하고 여기서 G1은 2'-데옥시-7-데아자구아노신이고, 선택적으로 올리고뉴클레오티드는 서열 5'-TCG1AACG1TTCG1-X-G1CTTG1CAAG1CT-5'(서열번호 32)을 포함하고, 여기서 G1은 2'-데옥시-7-데아자구아노신이고 X는 글리세롤(5'-서열번호 31-3'-X-3'-서열번호: 31-5')인, 조성물.
X6. 양태 X1-5 중 어느 하나에 있어서, 올리고뉴클레오티드는 적어도 하나의 포스포로티오에이트 연결을 포함하고, 선택적으로 모든 뉴클레오티드 연결은 포스포로티오에이트 연결인, 조성물.
X7. 양태 X1-6 중 어느 하나에 있어서, 올리고뉴클레오티드는 단일-가닥 올리고데옥시뉴클레오티드인, 조성물.
X8. 양태 X1-7 중 어느 하나에 있어서, 0.5ml 용량의 면역원성 조성물은 약 750 내지 약 3000μg의 올리고뉴클레오티드를 포함하고, 선택적으로 면역원성 조성물은 약 750μg, 약 1500μg, 또는 약 3000μg의 올리고뉴클레오티드를 포함하는, 조성물.
X9. 양태 X1-8 중 어느 하나에 있어서, SARS-CoV-2 항원이 불활성화된 전체 SARS-CoV-2인, 조성물.
X10. 양태 X9에 있어서, SARS-CoV-2는 포르말린 및 자외선 중 하나 또는 둘 모두로의 처리에 의해 불활성화되는, 조성물.
X11. 양태 X1-8 중 어느 하나에 있어서, SARS-CoV-2 항원은 SARS-CoV-2 스파이크(S) 단백질의 수용체-결합 도메인(RBD)을 포함하는, 조성물.
X12. 양태 X11에 있어서, SARS-CoV-2 항원은 전장 S 단백질의 신호 펩티드, 막횡단 및 세포질 도메인이 결핍된 절단된 재조합 S 단백질을 포함하는, 조성물.
X13. 양태 X11 또는 X12에 있어서, SARS-CoV-2 항원은 SARS-CoV-2 막(M) 단백질, 뉴클레오캡시드(N) 단백질, 및 엔벨로프(E) 단백질 중 하나 이상을 추가로 포함하는, 조성물.
X14. 양태 X1-13 중 어느 하나에 있어서, 알루미늄 염 어쥬번트를 추가로 포함하는, 조성물.
X15. 양태 X14에 있어서, 알루미늄 염 어쥬번트는 무정형 알루미늄 하이드록시포스페이트 설페이트, 수산화알루미늄, 인산알루미늄, 및 칼륨 알루미늄 설페이트로 구성된 군 중 하나 이상을 포함하는, 조성물.
X16. 양태 X14에 있어서, 알루미늄 염 어쥬번트는 수산화알루미늄을 포함하는, 조성물.
X17. 양태 X14-16 중 어느 하나에 있어서, 0.5ml 용량의 면역원성 조성물은 약 0.25 내지 약 0.50mg Al3+를 포함하는, 조성물.
X18. 양태 X1-17 중 어느 하나에 있어서, 포유동물 대상체는 인간 대상체인, 조성물.
X19. 다음을 포함하는 키트:
i) 양태 X1-18 중 어느 하나의 면역원성 조성물, 및
ii) 포유동물 대상체에서 SARS-CoV-2 항원에 대한 면역 반응을 자극하기 위한 조성물의 투여에 대한 지침.
X20. 양태 X19에 있어서, iii) 면역원성 조성물의 근육내 주사를 위한 주사기 및 바늘을 추가로 포함하는, 키트.
X21. 포유동물 대상체에서 SARS-CoV-2 항원에 대한 면역 반응을 자극하기 위해 양태 X1-18 중 어느 하나의 면역원성 조성물을 포유동물 대상체에게 투여하는 것을 포함하는, 포유동물 대상체에서 중증 급성 호흡기 증후군 코로나바이러스 2(SARS-CoV-2)에 대한 면역 반응을 자극하는 방법.
X22. 양태 X21에 있어서, 면역원성 조성물은 근육내 주사에 의해 투여되는, 방법.
본 출원은 US62/983,737(2020년 3월 1일), EP20168324.0(2020년 4월 6일), EP20202124.2(2020년 10월 15일), EP 202111936.8(2020년 12월 4일), EP21154645.2(2021년 2월 1일), PCT/US21/20313(2021년 3월 1일) 및 EP21160933.4(2021년 3월 5일)으로부터 우선권을 주장하며, 그 내용은 참고로 본 명세서에 포함된다. 상기 명세서에서 언급된 모든 간행물은 참고로 본 명세서에 포함된다. 본 발명의 기술된 실시형태의 다양한 변형 및 변경은 본 발명의 범주 및 사상을 벗어나지 않고 당업자에게 명백할 것이다. 본 발명이 특정 바람직한 실시형태와 관련하여 기술되었지만, 청구된 바와 같은 발명이 그러한 특정 실시형태에 과도하게 제한되어서는 안된다는 것을 이해해야 한다. 실제로, 당업자에게 자명한 발명을 수행하기 위한 기술된 양식의 다양한 변형은 다음 청구범위의 범주 내에 있는 것으로 의도된다.
서열
서열번호: 1
중증 급성 호흡기 증후군 코로나바이러스 2 (SARS-CoV-2) 단리물 Wuhan-Hu-1, 완전 게놈 (GenBank: MN908947; Wu, F., et al. A new coronavirus associated with human respiratory disease in China (2020) Nature 579:265-269)
ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
서열번호: 2
중증 급성 호흡기 증후군 코로나바이러스 2 orf1ab 다단백질의 단리물 Wuhan-Hu-1 (GenBank: QHD43415)
MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGGAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGGKIVNNWLKQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFANKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQSAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQAENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFSLWVYKQFDTYNLWNTFTRLQSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQSSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASSSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNN
서열번호: 3
중증 급성 호흡기 증후군 코로나바이러스 2 표면 당단백질 (GenBank: QHD43416)
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 4
CpG 1018
TGACTGTGAACGTTCGAGATGA
서열번호: 5
KLK 펩티드
KLKLLLLLKLK
서열번호: 6
Oligo-d(IC)13 (ODN1a)
ICICICICICICICICICICICICIC
서열번호: 7
CpG 1826
TCCATGACGTTCCTGACGTT
서열번호: 8
CpG 7909
TCGTCGTTTTGTCGTTTTGTCGTT
서열번호: 9
>hCoV-19/Italy/INMI1-isl/2020|EPI_ISL_410545|2020-01-29 (수탁번호: MT066156)
ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCTAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTNTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAA
GAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGTTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAT
서열번호: 10
>중증 급성 호흡기 증후군 코로나바이러스 2 orf1ab 다단백질의 단리물 hCoV-19/Italy/INMI1-isl/2020 (유전자은행 수탁번호: QIA98553)
MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGGAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGGKIVNNWLKQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFANKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQSAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFXYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQAVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQAENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFSLWVYKQFDTYNLWNTFTRLQSLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQSSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASSSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKE
서열번호: 11
>단백질\S_2019-nCoV/Italy-INMI1 (S단백질_hCoV19ItalyINMI1isl2020)(유전자은행 수탁번호: QIA98554)
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 12
>hCoV-19/France/IDF0372-isl/2020|EPI_ISL_410720|2020-01-23
ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATG
GTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTA
GTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACCTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTTTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGATGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGTTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGACAAAA
서열번호: 13
>중증 급성 호흡기 증후군 코로나바이러스 2 orf1ab 다단백질의 단리물 hCoV-19/France/IDF0372-isl/2020
MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGGAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGGKIVNNWLKQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFANKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQSAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSADAQSFLNGFAV
서열번호: 14
>단백질\S_인간\2019-nCoV (S단백질_hCoV19FranceIDF0372isl2020)
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSFLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 15
>hCoV-19/Austria/CeMM0360/2020|EPI_ISL_438123|2020-04-05
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAA
TTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGKCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTRATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGTTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGMCAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAATGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAAACGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGNATGACANNNNNNNNNNNNNN
서열번호: 16
>중증 급성 호흡기 증후군 코로나바이러스 2 orf1ab 다단백질의 단리물 hCoV-19/Austria/CeMM0360/2020
MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGGAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGGAPTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGGKIVNNWLKQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFANKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQSAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQSKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQAIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQNNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQAGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQSADAQSFLNGFAV
서열번호: 17
>SARS-CoV-2_S_MedUniWien (S단백질_hCoV19AustriaCeMM03602020)
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRXQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSXNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRVSANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 18
>중증 급성 호흡기 증후군 코로나바이러스 2 단리물 SARS-CoV-2/인간/England/ex-SA/2021, EVAg Ref-SKU:004V-04071 (SA_P2) 완전 게놈. South-African B.1.351 계통
ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCTTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACATCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACTAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGTTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAATTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTACAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATMTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTATTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAGGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTKTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACYTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGNNNNNNAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTAYTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTA
CACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGT
AGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAAC
ACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTTCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTG
ACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCC
TAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGCTAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGGTCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAANNNNNNCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAA
ATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAATATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTAAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTTATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGTAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCATAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTTAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTC
GTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCTTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGCGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTTATCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTT
CCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAATTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATC
AGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
서열번호: 19
>SA_P2_gp02 표면 당단백질, 게놈 수탁 SA_P2_t0.9_q20 유래
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFANPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRGLPQGFSALEPLVDLPIGINITRFQXXXLHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGVENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 20
>MW520923.1 중증 급성 호흡기 증후군 코로나바이러스 2 단리물 SARS-CoV-2/인간/USA/MN-MDH-2399/2021, 완전 게놈, 브라질 P1 계통의 예.
CAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTG
CACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTA
CGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCT
TGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCT
TTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAA
AAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGT
TATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATG
TGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGC
GCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGACTTTCAAGAAAACTGGAACACTAA
ACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCT
GTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAA
CAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACG
TTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTC
CAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATG
GGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGA
TCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTA
AAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAA
GTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTAT
TGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACA
TAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAG
AAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCAC
AAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAG
TTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCA
TCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAA
GGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGG
CTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTT
GGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGA
CGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAA
TTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGA
GCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAG
AGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAG
TGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCA
TTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACC
TAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGATACTGTGA
TAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGC
TCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCA
ACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGT
CTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAA
GAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGC
CACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAAC
AAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACA
CCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGA
CATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTG
TTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTT
AAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAA
AGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAG
CTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTC
TTTGATAAAAATCTCTATGACAAACTTGTTTTAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGC
TGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGA
AAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATT
GACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCC
ATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTG
AAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTAC
ACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAA
GCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTG
TCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGAT
TATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAAC
TCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAG
TGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCT
GAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGG
TATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTA
TCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATT
AACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGT
TACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGG
CTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAA
TACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCA
ACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTT
GTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAA
CATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGG
TGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTA
AACAAGCTACACAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAG
CATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAAC
TTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAA
ACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGAC
AATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAG
CTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTG
CTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCC
TCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAA
ACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCGGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAG
AGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATA
CAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTT
AAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTA
ATGAATTATCTAGAGTGTTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACT
ATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGT
TTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTA
AAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTAT
TTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAAT
CTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATT
TGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCT
TTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGT
TGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTT
TCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATT
TCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTG
TAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTA
GAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACA
TTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTAC
TGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAA
AGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCT
ATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCT
TATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGT
TTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAA
GCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTC
AGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTA
ATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGT
CATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACA
ACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTG
TTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACA
CTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAG
TGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAAC
ATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTC
ATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTT
CTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACAT
CAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAAT
GTACTAGAAGGTTCTGTTGCTTATGAAAATTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATT
TCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAA
GATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTT
TTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATC
AGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTT
TTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTT
TACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGC
ACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAA
AGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAA
GCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATA
TAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTT
GTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATC
ACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAAC
TTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAG
ACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAA
CTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTA
TAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAAT
GTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTAT
GACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTT
TTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGT
TGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCT
ATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGTCGT
TTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAG
AAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAG
GGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTT
TTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAAC
ATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCT
AGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGAAGCTAAAAGACTGTGTTATGTATGCATC
AGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATG
TCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTT
ACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCC
TATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACT
TTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTT
AGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGT
TGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCT
CAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTA
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACAT
AAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCAT
ATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTG
AAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCA
AGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTT
TCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAAC
ATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTAC
AACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAA
TTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAG
AATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAA
TGCGTTAGCTTATTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGG
CTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCT
AAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGC
TGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTG
TAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACA
CACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTG
TCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTA
CAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGC
TGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGT
GCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGT
AGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTT
ACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCT
GTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATA
CACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGATACATTAAAAGAAATACTTGTCACAT
ACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATAC
GCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGT
TGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTA
GTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCA
CATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACT
CTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGC
ATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTT
GATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACA
TAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTAC
TAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTT
AACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTT
TGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAAC
TACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTC
AACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGA
GGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTA
GTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTA
TTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTT
AAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACA
TGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTA
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATC
AGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTAT
CTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGA
GATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGC
TGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATC
AAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAA
CATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTG
TTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCAC
TTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTA
ACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGA
GGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTG
GTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTG
TCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTA
TTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACAT
GTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCT
AACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGG
TATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTA
ACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGT
GACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACA
TACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCA
ATATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCA
CCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTC
TCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCAC
GTGCTCGTGTAGATTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTG
CCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAG
ATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACAC
TAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGT
TGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCA
ATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAA
GAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCA
AAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCAC
TGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGT
CTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAA
AATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGT
TGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCT
CTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACAT
GTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCT
AGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAG
TTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTG
CGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGG
CTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACAT
GCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATT
GATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGT
AGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAAT
ATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTA
GCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAA
GTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACA
AATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGAC
ACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACC
AGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATG
GAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCT
GTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTG
GGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATG
TTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTT
GATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAA
CATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACA
AAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATT
TGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCT
TATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTG
GAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACT
CAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCAT
TGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTAC
ATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTT
AAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTT
TGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCAT
TTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTT
GCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATT
ACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCT
ATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTG
CCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGC
AACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA
ATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATA
AAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAA
TGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATG
TCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAA
TTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAG
TAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATG
TTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATTTTACAAACAGAACTCAATTACCCTCTGCATA
CACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGT
TCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAAC
CCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTAC
TACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAAT
TTTGTAATTATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCT
AGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAA
TCTTAGTGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTG
ATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTA
CTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGG
TTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACC
CTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAA
CCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGC
ATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTT
CCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTA
ATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAACGATTGCTGATTATAATTATAAATTACCAGATGA
TTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGAT
TGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAAT
GGTGTTAAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTTATGGTGTTGGTTACCAACCATA
CAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTA
AAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCT
TTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTAC
ACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAATCAGGTTGCTGTTCTTTATCAGGGTG
TTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAAT
GTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAATATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGC
AGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCT
ACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGT
GTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGA
ATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAG
ACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAAT
TTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACT
TGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGT
TTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACA
ATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGG
TATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTC
AAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACG
CTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGA
GGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAG
CTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTATTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGAT
TTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGT
CCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCT
TTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTT
GTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATT
CAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTT
CATTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAA
GAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGT
AATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCA
AATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTA
TGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCT
ACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCAGAG
CGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGT
TGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTA
GTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCC
ATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTT
CTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAA
AAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCA
ATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCC
AAATTCACACAATCGACGGTTCACCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACT
AGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGT
TAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTG
CGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCT
TCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATG
GCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATT
CCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCT
GGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCT
ATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTC
CATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAG
AAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATC
AAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGC
AGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTG
ACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATT
ACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTA
AGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATT
CTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTT
AAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTT
GCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCT
AAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAAC
ACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCT
GCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCT
AAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCA
TGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGC
TAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTA
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGAAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTC
TATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACAAACTAAAATGTCTGA
TAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATG
GAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTC
ACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCGAGATGACCA
AATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATT
TCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAG
GGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCA
AGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTA
GTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCTCTAAACGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGAT
GCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGG
CCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATG
TAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACT
GATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGA
AGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAG
TCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCT
GATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTT
CTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGG
GCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCA
CAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCAC
CACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGC
CCTAATGTGTAAAATTAATTTTAGTAGTGCTAACCCCATGTGATTTTAATAGCTTCTTA
서열번호: 21
>QQX12069.1 표면 당단백질, 게놈 수탁 MW520923 유래
MFVFLVLLPLVSSQCVNFTNRTQLPSAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNYPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLSEFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGTIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEYVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAAIKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASFVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 22
> 중증 급성 호흡기 증후군 코로나바이러스 2 단리물 SARS-CoV-2/인간/England/MIG457/2020, EVAg Ref-SKU:004V-04032, 완전 게놈. UK B 1.1.7 계통
ATTAAAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCTGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACACCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTATTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAATCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGATAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTTACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGTTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTACAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCARTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTC
CATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCTGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCATCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACCTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAG
TTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAG
TGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAGGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTAC
AAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATA
CCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAAC
ACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGGATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGATATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTC
TAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTACCACAAAAACAACAAAAGTTGGATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCTGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTTATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGATGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCATCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCATAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTGCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACACACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCAGAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTCCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCAT
ACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTTAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTATAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTGCATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAATGTCTCTAAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAAACGAACTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTTTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTA
AAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
서열번호: 23
> MIG457_gp02 QQH18545.1 표면 당단백질, UK_MIG457 게놈 수탁 MW422256 유래
MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAISGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIDDTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSHRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPINFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILARLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTHNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 24
>MW493681.1 중증 급성 호흡기 증후군 코로나바이러스 2 단리물 SARS-CoV-2/인간/USA/NMDOH-2021013232/2021, 완전 게놈. [중증 급성 호흡기 증후군 코로나바이러스 2 (SARS-CoV-2)], 캘리포니아 B.1.427 계통
AAGGTTTATACCTTCCCAGGTAACAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTT
GACATCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTG
CTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTT
GGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTCACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGT
GTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATT
CTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTATGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGTCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCT
TTGCATCATTTTATTATGTATGGAAAAGTTATGTGCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTACTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTG
TAGCTTATTTTAATATGGTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAAAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAATTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAAGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACCTTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTA
GCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCTAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCATATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTATTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGTATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTA
TAATTATAAATTACCAGATGATTTTACAGGCTGCGTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCGGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGTGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCATAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGCTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTCTACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTAT
GATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGTAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTTCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTATAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGT
GACGGTAAAATGAAAGATCTCAGTCCAAGATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAATTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGG
AAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTTCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCC
서열번호: 25
>QQV21856.1: S 표면 단백질
MFVFLVLLPLVSIQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSCMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 26
>MW306426.1 중증 급성 호흡기 증후군 코로나바이러스 2 단리물 SARS-CoV-2/인간/USA/CA-CZB-12872/2020, 완전 게놈. [중증 급성 호흡기 증후군 코로나바이러스 2 (SARS-CoV-2)]. 캘리포니아 B.1.429 계통
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTGGCTGTCACTCGGCTGCATGCTTAGTGCACTCACGCAGTATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCTATCTTCTGCAGGCTGCTTACGGTTTCGTCCGTGTTGCAGCCGATCATCAGCACATCTAGGTTTTGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTCCCTGGTTTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTTTTACAGGTTCGCGACGTGCTCGTACGTGGCTTTGGAGACTCCGTGGAGGAGGTCTTATCAGAGGCACGTCAACATCTTAAAGATGGCACTTGTGGCTTAGTAGAAGTTGAAAAAGGCGTTTTGCCTCAACTTGAACAGCCCTATGTGTTCATCAAACGTTCGGATGCTCGAACTGCACCTCATGGTCATGTTATGGTTGAGCTGGTAGCAGAACTCGAAGGCATTCAGTACGGTCGTAGTGGTGAGACACTTGGTGTCCTTGTCCCTCATGTGGGCGAAATACCAGTGGCTTACCGCAAGGTTCTTCTTCGTAAGAACGGTAATAAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTTAGGCGACGAGCTTGGCACTGATCCTTATGAAGATTTTCAAGAAAACTGGAACACTAAACATAGCAGTGGTGTTACCCGTGAACTCATGCGTGAGCTTAACGGAGGGGCATACACTCGCTATGTCGATAACAACTTCTGTGGCCCTGATGGCTACCCTCTTGAGTGCATTAAAGACCTTCTAGCACGTGCTGGTAAAGCTTCATGCACTTTGTCCGAACAACTGGACTTTATTGACACTAAGAGGGGTGTATACTGCTGCCGTGAACATGAGCATGAAATTGCTTGGTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAATTAAATTGGCAAAGAAATTTGACATCTTCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTATGGGTAGAATTCGATCTGTCTATCCAGTTGCGTCACCAAATGAATGCAACCAAATGTGCCTTTCAACTCTCATGAAGTGTGATCATTGTGGTGAAACTTCATGGCAGACGGGCGATTTTGTTAAAGCCACTTGCGAATTTTGTGGCACTGAGAATTTGACTAAAGAAGGTGCCACTACTTGTGGTTACTTACCCCAAAATGCTGTTGTTAAAATTTATTGTCCAGCATGTCACAATTCAGAAGTAGGACCTGAGCATAGTCTTGCCGAATACCATAATGAATCTGGCTTGAAAACCATTCTTCGTAAGGGTGGTCGCACTATTGCCTTTGGAGGCTGTGTGTTCTCTTATGTTGGTTGCCATAACAAGTGTGCCTATTGGGTTCCACGTGCTAGCGCTAACATAGGTTGTAACCATACAGGTGTTGTTGGAGAAGGTTCCGAAGGTCTTAATGACAACCTTCTTGAAATACTCCAAAAAGAGAAAGTCAACATCAATATTGTTGGTGACTTTAAACTTAATGAAGAGATCGCCATTATTTTGGCATCTTTTTCTGCTTCCACAAGTGCTTTTGTGGAAACTGTGAAAGGTTTGGATTATAAAGCATTCAAACAAATTGTTGAATCCTGTGGTAATTTTAAAGTTACAAAAGGAAAAGCTAAAAAAGGTGCCTGGAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTCCCGCACTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATAACAATACTAGATGGAATTTCACAGTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTACTAACAATCTAGTTGTAATGGCCTACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATCTTTGGCACTGTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAAGGAAGGTGTAGAGTTTCTTAGAGACGGTTGGGAAATTGTTAAATTTATCTCAACCTGTGCTTGTGAAATTGTCGGTGGACAAATTGTCACCTGTGCAAAGGAAATTAAGGAGAGTGTTCAGACATTCTTTAAGCTTGTAAATAAATTTTTGGCTTTGTGTGCTGACTCTATCATTATTGGTGGAGCTAAACTTAAAGCCTTGAATTTAGGTGAAACATTTGTTACGCACTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTCTAAAAGCCCCAAAAGAAATTATCTTCTTAGAGGGAGAAACACTTCCCACAGAAGTGTTAACAGAGGAAGTTGTCTTGAAAACTGGTGATTTACAACCATTAGAACAACCTACTAGTGAAGCTGTTGAAGCTCCACTGGTTGGTACACCAGTTTGTATTAACGGGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTTCACACTCAAAGGCGGTGCACCAACAAAGGTTACTTTTGGTGATGACACTGTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAAGTACTTAATGAGAAGTGCTCTGCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGTGGCAGATGCTGTCATAAAAACTTTGCAACCAGTATCTGAATTACTTACACCACTGGGCATTGATTTAGATGAGTGGAGTATGGCTACATACTACTTATTTGATGAGTCTGGTGAGTTTAAATTGGCTTCACATATGTATTGTTCTTTTTACCCTCCAGA
TGAGGATGAAGAAGAAGGTGATTGTGAAGAAGAAGAGTTTGAGCCATCAACTCAATATGAGTATGGTACTGAAGATGATTACCAAGGTAAACCTTTGGAATTTGGTGCCACTTCTGCTGCTCTTCAACCTGAAGAAGAGCAAGAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGACGGCAGTGAGGACAATCAGACAACTACTATTCAAACAATTGTTGAGGTTCAACCTCAATTAGAGATGGAACTTACACCAGTTGTTCAGACTATTGAAGTGAATAGTTTTAGTGGTTATTTAAAACTTACTGACAATGTATACATTAAAAATGCAGACATTGTGGAAGAAGCTAAAAAGGTAAAACCAACAGTGGTTGTTAATGCAGCCAATGTTTACCTTAAACATGGAGGAGGTGTTGCAGGAGCCTTAAATAAGGCTACTAACAATGCCATGCAAGTTGAATCTGATGATTACATAGCTACTAATGGACCACTTAAAGTGGGTGGTAGTTGTGTTTTAAGCGGACACAATCTTGCTAAACACTGTCTTCATGTTGTCGGCCCAAATGTTAACAAAGGTGAAGACATTCAACTTCTTAAGAGTGCTTATGAAAATTTTAATCAGCACGAAGTTCTACTTGCACCATTATTATCAGCTGGTATTTTTGGTGCTGACCCTATACATTCTTTAAGAGTTTGTGTAGATACTGTTCGCACAAATGTCTACTTAGCTGTCTTTGATAAAAATCTCTATGACAAACTTGTTTCAAGCTTTTTGGAAATGAAGAGTGAAAAGCAAGTTGAACAAAAGATCGCTGAGATTCCTAAAGAGGAAGTTAAGCCATTTATAACTGAAAGTAAACCTTCAGTTGAACAGAGAAAACAAGATGATAAGAAAATCAAAGCTTGTGTTGAAGAAGTTACAACAACTCTGGAAGAAACTAAGTTCCTCACAGAAAACTTGTTACTTTATATTGACATTAATGGCAATCTTCATCCAGATTCTGCCACTCTTGTTAGTGACATTGACATCACTTTCTTAAAGAAAGATGCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGTGGTTATACCTACTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAAGTGCCAACAGACAATTATATAACCACTTACCCGGGTCAGGGTTTAAATGGTTACACTGTAGAGGAGGCAAAGACAGTGCTTAAAAAGTGTAAAAGTGCCTTTTACATTCTACCATCTATTATCTCTAATGAGAAGCAAGAAATTCTTGGAACTGTTTCTTGGAATTTGCGAGAAATGCTTGCACATGCAGAAGAAACACGCAAATTAATGCCTGTCTGTGTGGAAACTAAAGCCATAGTTTCAACTATACAGCGTAAATATAAGGGTATTAAAATACAAGAGGGTGTGGTTGATTATGGTGCTAGATTTTACTTTTACACCAGTAAAACAACTGTAGCGTCACTTATCAACACACTTAACGATCTAAATGAAACTCTTGTTACAATGCCACTTGGCTATGTAACACATGGCTTAAATTTGGAAGAAGCTGCTCGGTATATGAGATCTCTCAAAGTGCCAGCTACAGTTTCTGTTTCTTCACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAACACCTGAAGAACATTTTATTGAAACCATCTCACTTGCTGGTTCCTATAAAGATTGGTCCTATTCTGGACAATCTACACAACTAGGTATAGAATTTCTTAAGAGAGGTGATAAAAGTGTATATTACACTAGTAATCCTACCACATTCCACCTAGATGGTGAAGTTATCACCTTTGACAATCTTAAGACACTTCTTTCTTTGAGAGAAGTGAGGACTATTAAGGTGTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGTCAATGACATATGGACAACAGTTTGGTCCAACTTATTTGGATGGAGCTGATGTTACTAAAATAAAACCTCATAATTCACATGAAGGTAAAACATTTTATGTTTTACCTAATGATGACACTCTACGTGTTGAGGCTTTTGAGTACTACCACACAACTGATCCTAGTTTTCTGGGTAGGTACATGTCAGCATTAAATCACACTAAAAAGTGGAAATACCCACAAGTTAATGGTTTAACTTCTATTAAATGGGCAGATAACAACTGTTATCTTGCCACTGCATTGTTAACACTCCAACAAATAGAGTTGAAGTTTAATCCACCTGCTCTACAAGATGCTTATTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAGCCTACTGTAATAAGACAGTAGGTGAGTTAGGTGATGTTAGAGAAACAATGAGTTACTTGTTTCAACATGCCAATTTAGATTCTTGCAAAAGAGTCTTGAACGTGGTGTGTAAAACTTGTGGACAACAGCAGACAACCCTTAAGGGTGTAGAAGCTGTTATGTACATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGTACGTGTGGTAAACAAGCTACAAAATATCTAGTACAACAGGAGTCACCTTTTGTTATGATGTCAGCACCACCTGCTCAGTATGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTAATTACCAGTGTGGTCACTATAAACATATAACTTCTAAAGAAACTTTGTATTGCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATTACGGATGTTTTCTACAAAGAAAACAGTTACACAACAACCATAAAACCAGTTACTTATAAATTGGATGGTGTTGTTTGTACAGAAATTGACCCTAAGTTGGACAATTATTATAAGAAAGACAATTCTTATTTCACAGAGCAACCAATTGATCTTGTACCAAACCAACCATATCCAAACGCAAGCTTCGATAATTTTAAGTTTGTA
TGTGATAATATCAAATTTGCTGATGATTTAAACCAGTTAACTGGTTATAAGAAACCTGCTTCAAGAGAGCTTAAAGTTACATTTTTCCCTGACTTAAATGGTGATGTGGTGGCTATTGATTATAAACACTACACACCCTCTTTTAAGAAAGGAGCTAAATTGTTACATAAACCTATTGTTTGGCATGTTAACAATGCAACTAATAAAGCCACGTATAAACCAAATACCTGGTGTATACGTTGTCTTTGGAGCACAAAACCAGTTGAAACATCAAATTCGTTTGATGTACTGAAGTCAGAGGACGCGCAGGGAATGGATAATCTTGCCTGCGAAGATCTAAAACCAGTCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAAGACGTTCTTGAGTGTAATGTGAAAACTACCGAAGTTGTAGGAGACATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGGCCACACAGATCTAATGGCTGCTTATGTAGACAATTCTAGTCTTACTATTAAGAAACCTAATGAATTATCTAGAGTATTAGGTTTGAAAACCCTTGCTACTCATGGTTTAGCTGCTGTTAATAGTGTCCCTTGGGATACTATAGCTAATTATGCTAAGCCTTTTCTTAACAAAGTTGTTAGTACAACTACTAACATAGTTACACGGTGTTTAAACCGTGTTTGTACTAATTATATGCCTTATTTCTTTACTTTATTGCTACAATTGTGTACTTTTACTAGAAGTACAAATTCTAGAATTAAAGCATCTATGCCGACTACTATAGCAAAGAATACTGTTAAGAGTGTCGGTAAATTTTGTCTAGAGGCTTCATTTAATTATTTGAAGTCACCTAATTTTTCTAAACTGATAAATATTATAATTTGGTTTTTACTATTAAGTGTTTGCCTAGGTTCTTTAATCTACTCAACCGCTGCTTTAGGTGTTTTAATGTCTAATTTAGGCATGCCTTCTTACTGTACTGGTTACAGAGAAGGCTATTTGAACTCTACTAATGTCACTATTGCAACCTACTGTACTGGTTCTATACCTTGTAGTGTTTGTCTTAGTGGTTTAGATTCTTTAGACACCTATCCTTCTTTAGAAACTATACAAATTACCATTTCATCTTTTAAATGGGATTTAACTGCTTTTGGCTTAGTTGCAGAGTGGTTTTTGGCATATATTCTTTTCACTAGGTTTTTCTATGTACTTGGATTGGCTGCAATCATGCAATTGTTTTTCAGCTATTTTGCAGTACATTTTATTAGTAATTCTTGGCTTATGTGGTTAATAATTAATCTTGTACAAATGGCCCCGATTTCAGCTATGGTTAGAATGTACATCTTCTTTGCATCATTTTATTATGTATGGAAAAGTTATGT
GCATGTTGTAGACGGTTGTAATTCATCAACTTGTATGATGTGTTACAAACGTAATAGAGCAACAAGAGTCGAATGTACAACTATTGTTAATGGTGTTAGAAGGTCCTTTTATGTCTATGCTAATGGAGGTAAAGGCTTTTGCAAACTACACAATTGGAATTGTGTTAATTGTGATACATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGTTGCGAGAGACTTGTCACTACAGTTTAAAAGACCAATAAATCCTACTGACCAGTCTTCTTACATCGTTGATAGTGTTACAGTGAAGAATGGTTCCATCCATCTTTACTTTGATAAAGCTGGTCAAAAGACTTATGAAAGACATTCTCTCTCTCATTTTGTTAACTTAGACAACCTGAGAGCTAATAACACTAAAGGTTCATTGCCTATTAATGTTATAGTTTTTGATGGTAAATCAAAATGTGAAGAATCATCTGCAAAATCAGCGTCTGTTTACTACAGTCAGCTTATGTGTCAACCTATACTGTTACTAGATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAAAATGTTTGATGCTTACGTTAATACGTTTTCATCAACTTTTAACGTACCAATGGAAAAACTCAAAACACTAGTTGCAACTGCAGAAGCTGAACTTGCAAAGAATGTGTCCTTAGACAATGTCTTATCTACTTTTATTTCAGCAGCTCGGCAAGGGTTTGTTGATTCAGATGTAGAAACTAAAGATGTTGTTGAATGTCTTAAATTGTCACATCAATCTGACATAGAAGTTACTGGCGATAGTTGTAATAACTATATGCTCACCTATAACAAAGTTGAAAACATGACACCCCGTGACCTTGGTGCTTGTATTGACTGTAGTGCGCGTCATATTAATGCGCAGGTAGCAAAAAGTCACAACATTGCTTTGATATGGAACGTTAAAGATTTCATGTCATTGTCTGAACAACTACGAAAACAAATACGTAGTGCTGCTAAAAAGAATAACTTACCTTTTAAGTTGACATGTGCAACTACTAGACAAGTTGTTAATGTTGTAACAACAAAGATAGCACTTAAGGGTGGTAAAATTGTTAATAATTGGTTGAAGCAGTTAATTAAAGTTACACTTGTGTTCCTTTTTGTTGCTGCTATTTTCTATTTAATAACACCTGTTCATGTCATGTCTAAACATACTGACTTTTCAAGTGAAATCATAGGATACAAGGCTATTGATGGTGGTGTCACTCGTGACATAGCATCTACAGATACTTGTTTTGCTAACAAACATGCTGATTTTGACACATGGTTTAGCCAGCGTGGTGGTAGTTATACTAATGACAAAGCTTGCCCATTGATTGCTGCAGTCATAACAAGAGAAGTGGGTTTTGTCGTGCCTGGTTTGCCTGGCACGATATTACGCACAACTAATGGTGACTTTTTGCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAATATCTGTTACACACCATCAAAACTTATAGAGTACACTGACTTTGCAACATCAGCTTGTGTTTTGGCTGCTGAATGTACAATTTTTAAAGATGCTTCTGGTAAGCCAGTACCATATTGTTATGATACCAATGTACTAGAAGGTTCTGTTGCTTATGAAAGTTTACGCCCTGACACACGTTATGTGCTCATGGATGGCTCTATTATTCAATTTCCTAACACCTACCTTGAAGGTTCTGTTAGAGTGGTAACAACTTTTGATTCTGAGTACTGTAGGCACGGCACTTGTGAAAGATCAGAAGCTGGTGTTTGTGTATCTACTAGTGGTAGATGGGTACTTAACAATGATTATTACAGATCTTTACCAGGAGTTTTCTGTGGTGTAGATGCTGTAAATTTACTTACTAATATGTTTACACCACTAATTCAACCTATTGGTGCTTTGGACATATCAGCATCTATAGTAGCTGGTGGTATTGTAGCTATCGTAGTAACATGCCTTGCCTACTATTTTATGAGGTTTAGAAGAGCTTTTGGTGAATACAGTCATGTAGTTGCCTTTAATACTTTACTATTCCTTATGTCATTCACTGTACTCTGTTTAACACCAGTTTACTCATTCTTACCTGGTGTTTATTCTGTTATTTACTTGTACTTGACATTTTATCTTACTAATGATGTTTCTTTTTTAGCACATATTCAGTGGATGGTTATGTTCACACCTTTAGTACCTTTCTGGATAACAATTGCTTATATCATTTGTATTTCCACAAAGCATTTCTATTGGTTCTTTAGTAATTACCTAAAGAGACGTGTAGTCTTTAATGGTGTTTCCTTTAGTACTTTTGAAGAAGCTGCGCTGTGCACCTTTTTGTTAAATAAAGAAATGTATCTAAAGTTGCGTAGTGATGTGCTATTACCTCTTACGCAATATAATAGATACTTAGCTCTTTATAATAAGTACAAGTATTTTAGTGGAGCAATGGATACAACTAGCTACAGAGAAGCTGCTTGTTGTCATCTCGCAAAGGCTCTCAATGACTTCAGTAACTCAGGTTCTGATGTTCTTTACCAACCACCACAAACCTCTATCACCTCAGCTGTTTTGCAGAGTGGTTTTAGAAAAATGGCATTCCCATCTGGTAAAGTTGAGGGTTGTATGGTACAAGTAACTTGTGGTACAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGACATGTGATCTGCACCTCTGAAGACATGCTTAACCCTAATTATGAAGATTTACTCATTCGTAAGTCTAATCATAATTTCTTGGTACAGGCTGGTAATGTTCAACTCAGGGTTATTGGACATTCTATGCAAAATTGTGTACTTAAGCTTAAGGTTGATACAGCCAATCCTAAGACACCTAAGTATAAGTTTGTTCGCATTCAACCAGGACAGACTTTTTCAGTGTTAGCTTGTTACAATGGTTCACCATCTGGTGTTTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTGTCTCTTTTTGTTACATGCACCATATGGAATTACCAACTGGAGTTCATGCTGGCACAGACTTAGAAGGTAACTTTTATGGACCTTTTGTTGACAGGCAAACAGCACAAGCAGCTGGTACGGACACAACTATTACAGTTAATGTTTTAGCTTGGTTGTACGCTGCTGTTATAAATGGAGACAGGTGGTTTCTCAATCGATTTACCACAACTCTTAATGACTTTAACCTTGTGGCTATGAAGTACAATTATGAACCTCTAACACAAGACCATGTTGACATACTAGGACCTCTTTCTGCTCAAACTGGAATTGCCGTTTTAGATATGTGTGCTTCATTAAAAGAATTACTGCAAAATGGTATGAATGGACGTACCATATTGGGTAGTGCTTTATTAGAAGATGAATTTACACCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGTGAAAAGAACAATCAAGGGTACACACCACTGGTTGTTACTCACAATTTTGACTTCACTTTTAGTTTTAGTCCAGAGTACTCAATGGTCTTTGTTCTTTTTTTTGTATGAAAATGCCTTTTTACCTTTTGCTATGGGTATTATTGCTATGTCTGCTTTTGCAATGATGTTTGTCAAACATAAGCATGCATTTCTCTGTTTGTTTTTGTTACCTTCTCTTGCCACTGTAGCTTATTTTAATATGGTCTATATGCCTGCTAG
TTGGGTGATGCGTATTATGACATGGTTGGATATGGTTGATACTAGTTTGTCTGGTTTTAAGCTAAAAGACTGTGTTATGTATGCATCAGCTGTAGTGTTACTAATCCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGACACTTATGAATGTCTTGACACTCGTTTATAAAGTTTATTATGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTCTAACTACTCAGGTGTAGTTACAACTGTCATGTTTTTGGCCAGAGGTATTGTTTTTATGTGTGTTGAGTATTGCCCTATTTTCTTCATAACTGGTAATACACTTCAGTGTATAATGCTAGTTTATTGTTTCTTAGGCTATTTTTGTACTTGTTACTTTGGCCTCTTTTGTTTACTCAACCGCTACTTTAGACTGACTCTTGGTGTTTATGATTACTTAGTTTCTACACAGGAGTTTAGATATATGAATTCACAGGGACTACTCCCACCCAAGAATAGCATAGATGCCTTCAAACTCAACATTAAATTGTTGGGTGTTGGTGGCAAACCTTGTATCAAAGTAGCCACTGTACAGTCTAAAATGTCAGATGTAAAGTGCACATCAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCTAAATTGTGGGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCTATAGCTTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGTAAGTTGGAAAAGATGGCTGATCAAGCTATGACCCAAATGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGCAGATAGTAAAATTGTTCAACTTAGTGAAATTAGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTTAAGGGCCAATTCTGCTGTCAAATTACAGAATAATGAGCTTAGTCCTGTTGCACTACGACAGATGTCTTGTGCTGCCGGTACTACACAAACTGCTTGCACTGATGACAATGCGTTAGCTTACTACAACACAACAAAGGGAGGTAGGTTTGTACTTGCACTGTTATCCGATTTACAGGATTTGAAATGGGCTAGATTCCCTAAGAGTGATGGAACTGGTACTGTCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCTAAAGGTCCTAAAGTGAAGTATTTATACTTTATTAAAGGATTAAACAACCTAAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGATTATCTAGCTAGTGGGGGACAACCAATCACTAATTGTGTTAAGATGTTGTGTACACACACTGGTACTGGTCAGGCAATAACAGTTACA
CCGGAAGCCAATATGGATCAAGAATCCTTTGGTGGTGCATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCAAATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTACAAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAAGGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGCTTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAATGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAATTGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAATTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAACTACCAACATGAAGAAACAATTTATAATTTACTTAAGGATTGTCCAGCTGTTGCTAAACATGACTTCTTTAAGTTTAGAATAGACGGTGACATGGTACCACATATATCACGTCAACGTCTTACTAAATACACAATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATTGTGACACATTAAAAGAAATACTTGTCACATACAATTGTTGTGATGATGATTATTTCAATAAAAAGGACTGGTATGATTTTGTAGAAAACCCAGATATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTGGTGTACTGACATTAGATAATCAAGATCTCAATGGTAACTGGTATGATTTCGGTGATTTCATACAAACCACGCCAGGTAGTGGAGTTCCTGTTGTAGATTCTTATTATTCATTGTTAATGCCTATATTAACC
TTGACCAGGGCTTTAACTGCAGAGTCACATGTTGACACTGACTTAACAAAGCCTTACATTAAGTGGGATTTGTTAAAATATGACTTCACGGAAGAGAGGTTAAAACTCTTTGACCGTTATTTTAAATATTGGGATCAGACATACCACCCAAATTGTGTTAACTGTTTGGATGACAGATGCATTCTGCATTGTGCAAACTTTAATGTTTTATTCTCTACAGTGTTCCCACTTACAAGTTTTGGACCACTAGTGAGAAAAATATTTGTTGATGGTGTTCCATTTGTAGTTTCAACTGGATACCACTTCAGAGAGCTAGGTGTTGTACATAATCAGGATGTAAACTTACATAGCTCTAGACTTAGTTTTAAGGAATTACTTGTGTATGCTGCTGACCCTGCTATGCACGCTGCTTCTGGTAATCTATTACTAGATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAACAAAGACTTCTATGACTTTGCTGTGTCTAAGGGTTTCTTTAAGGAAGGAAGTTCTGTTGAATTAAAACACTTCTTCTTTGCTCAGGATGGTAATGCTGCTATCAGCGATTATGACTACTATCGTTATAATCTACCAACAATGTGTGATATCAGACAACTACTATTTGTAGTTGAAGTTGTTGATAAGTACTTTGATTGTTACGATGGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATCAGCTGGTTTTCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGATTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCATATACAAAACGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACCAATAGACAGTTTCATCAAAAATTATTGAAATCAATAGCCGCCACTAGAGGAGCTACTGTAGTAATTGGAACAAGCAAATTCTATGGTGGTTGGCACAACATGTTAAAAACTGTTTATAGTGATGTAGAAAACCCTCACCTTATGGGTTGGGATTATCCTAAATGTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTGCGTAAACATTTCTCAATGATGATACTCTCTGACGATGCTGTTGTGTGTTTCAATAGCACTTATGCATCTCAAGGTCTAGTGGCTAGCATAAAGAACTTTAAGTCAGTTCTTTATTATCAAAACAATGTTTTTATGTCTGAAGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCATGAATTTTGCTCTCAACATACAATGCTAGTTAAACAGGGTGATGATTATGTGTACCTTCCTTACCCAGATCCATCAAGAATCCTAGGGGCCGGCTGTTTTGTAGATGATATCGTAAAAACAGATGGTACACTTATGATTGAACGGTTCGTGTCTTTAGCTATAGATGCTTACCCACTTACTAAACATCCTAATCAGGAGTATGCTGATGTCTTTCATTTGTACTTACAATACATAAGAAAGCTACATGATGAGTTAACAGGACACATGTTAGACATGTATTCTGTTATGCTTACTAATGATAACACTTCAAGGTATTGGGAACCTGAGTTTTATGAGGCTATGTACACACCGCATACAGTCTTACAGGCTGTTGGGGCTTGTGTTCTTTGCAATTCACAGACTTCATTAAGATGTGGTGCTTGCATACGTAGACCATTCTTATGTTGTAAATGCTGTTACGACCATGTCATATCAACATCACATAAATTAGTCTTGTCTGTTAATCCGTATGTTTGCAATGCTCCAGGTTGTGATGTCACAGATGTGACTCAACTTTACTTAGGAGGTATGAGCTATTATTGTAAATCACATAAACCACCCATTAGTTTTCCATTGTGTGCTAATGGACAAGTTTTTGGTTTATATAAAAATACATGTGTTGGTAGCGATAATGTTACTGACTTTAATGCAATTGCAACATGTGACTGGACAAATGCTGGTGATTACATTTTAGCTAACACCTGTACTGAAAGACTCAAGCTTTTTGCAGCAGAAACGCTCAAAGCTACTGAGGAGACATTTAAACTGTCTTATGGTATTGCTACTGTACGTGAAGTGCTGTCTGACAGAGAATTACATCTTTCATGGGAAGTTGGTAAACCTAGACCACCACTTAACCGAAATTATGTCTTTACTGGTTATCGTGTAACTAAAAACAGTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCATATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTTGGTATGCAAAAGTATTCTACACTCCAGGGACCACCTGGTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTGATGCACTATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTATACCTGCACGTGCTCGTGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACATTAGAACAGTATGTCTTTTGTACTGTAAATGCATTGCCTGAGACGACAGCAGATATAGTTGTCTTTGATGAAATTTCAATGGCCACAAATTATGATTTGAGTGTTGTCAATGCCAGATTACGTGCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGATAATAAGCTTAAAGCACATAAAGACAAATCAGCTCAATGCTTTAAAATGTTTTATAAGGGTGTTATCACGCATGATGTTTCATCTGCAATTAACAGGCCACAAATAGGCGTGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTTATTTCACCTTATAATTCACAGAATGCTGTAGCCTCAAAGATTTTGGGACTACCAACTCAAACTGTTGATTCATCACAGGGCTCAGAATATGACTATGTCATATTCACTCAAACCACTGAAACAGCTCACTCTTGTAATGTAAACAGATTTAATGTTGCTATTACCAGAGCAAAAGTAGGCATACTTTGCATAATGTCTGATAGAGACCTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTACAAGCTGAAAATGTAACAGGACTCTTTAAAGATTGTAGTAAGGTAATCACTGGGTTACATCCTACACAGGCACCTACACACCTCAGTGTTGACACTAAATTCAAAACTGAAGGTTTATGTGTTGACATACCTGGCATACCTAAGGACATGACCTATAGAAGACTCATCTCTATGATGGGTTTTAAAATGAATTATCAAGTTAATGGTTACCCTAACATGTTTATCACCCGCGAAGAAGCTATAAGACATGTACGTGCATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTTGGTACCAATTTACCTTTACAGCTAGGTTTTTCTACAGGTGTTAACCTAGTTGCTGTACCTACAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACACCTCATACCACTTATGTACAAAGGACTTCCTTGGAATGTAGTGCGTATAAAGATTGTACAAATGTTAAGTGACACACTTAAAAATCTCTCTGACAGAGTCGTATTTGTCTTATGGGCACATGGCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTATGCCTGTTGGCATCATTCTATTGGATTTGATTACGTCTATAATCCGTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCAACCATGATCTGTATTGTCAAGTCCATGGTAATGCACATGTAGCTAGTTGTGATGCAATCATGACTAGGTGTCTAGCTGTCCACGAGTGCTTTGTTAAGCGTGTTGACTGGACTATTGAATATCCTATAATTGGTGATGAACTGAAGATTAATGCGGCTTGTAGAAAGGTTCAACACATGGTTGTTAAAGCTGCATTATTAGCAGACAAATTCCCAGTTCTTCACGACATTGGTAACCCTAAAGCTATTAAGTGTGTACCTCAAGCTGATGTAGAATGGAAGTTCTATGATGCACAGCCTTGTAGTGACAAAGCTTATAAAATAGAAGAATTATTCTATTCTTATGCCACACATTCTGACAAATTCACAGATGGTGTATGCCTATTTTGGAATTGCAATGTCGATAGATATCCTGCTAATTCCATTGTTTGTAGATTTGACACTAGAGTGCTATCTAACCTTAACTTGCCTGGTTGTGATGGTGGCAGTTTGTATGTAAATAAACATGCATTCCACACACCAGCTTTTGATAAAAGTGCTTTTGTTAATTTAAAACAATTACCATTTTTCTATTACTCTGACAGTCCATGTGAGTCTCATGGAAAACAAGTAGTGTCAGATATAGATTATGTACCACTAAAGTCTGCTACGTGTATAACACGTTGCAATTTAGGTGGTGCTGTCTGTAGACATCATGCTAATGAGTACAGATTGTATCTCGATGCTTATAACATGATGATCTCAGCTGGCTTTAGCTTGTGGGTTTACAAACAATTTGATACTTATAACCTCTGGAACACTTTTACAAGACTTCAGAGTTTAGAAAATGTGGCTTTTAATGTTGTAAATAAGGGACACTTTGATGGACAACAGGGTGAAGTACCAGTTTCTATCATTAATAACACTGTTTACACAAAAGTTGATGGTGTTGATGTAGAATTGTTTGAAAATAAAACAACATTACCTGTTAATGTAGCATTTGAGCTTTGGGCTAAGCGCAACATTAAACCAGTACCAGAGGTGAAAATACTCAATAATTTGGGTGTGGACATTGCTGCTAATACTGTGATCTGGGACTACAAAAGAGATGCTCCAGCACATATATCTACTATTGGTGTTTGTTCTATGACTGACATAGCCAAGAAACCAACTGAAACGATTTGTGCACCACTCACTGTCTTTTTTGATGGTAGAGTTGATGGTCAAGTAGACTTATTTAGAAATGCCCGTAATGGTGTTCTTATTACAGAAGGTAGTGTTAAAGGTTTACAACCATCTGTAGGTCCCAAACAAGCTAGTCTTAATGGAGTCACATTAATTGGAGAAGCCGTAAAAACACAGTTCAATTATTATAAGAAAGTTGATGGTGTTGTCCAACAATTACCTGAAACTTACTTTACTCAGAGTAGAAATTTACAAGAATTTAAACCCAGGAGTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACATCTACTGATTGGACTAGCTAAACGTTTTAAGGAATCACCTTTTGAATTAGAAGATTTTATTCCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGGTTCATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTGAAATAATAAAATCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAGTGCAACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCAGGTACAGCTGTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCTTAATGACTTTGTCTCTGATGCAGATTCAACTTTGATTGGTGATTGTGCAACTGTACATACAGCTAATAAATGGGATCTCATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAAATGACTCTAAAGAGGGTTTTTTCACTTACATTTGTGGGTTTATACAACAAAAGCTAGCTCTTGGAGGTTCCGTGGCTATAAAGATAACAGAACATTCTTGGAATGCTGATCTTTATAAGCTCATGGGACACTTCGCATGGTGGACAGCCTTTGTTACTAATGTGAATGCGTCATCATCTGAAGCATTTTTAATTGGATGTAATTATCTTGGCAAACCACGCGAACAAATAGATGGTTATGTCATGCATGCAAATTACATATTTTGGAGGAATACAAATCCAATTCAGTTGTCTTCCTATTCTTTATTTGACATGAGTAAATTTCCCCTTAAATTAAGGGGTACTGCTGTTATGTCTTTAAAAGAAGGTCAAATCAATGATATGATTTTATCTCTTCTTAGTAAAGGTAGACTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGTTAACAACTAAACGAACAATGTTTGTTTTTCTTGTTTTATTGCCACTAGTCTCTATTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCATACACTAATTCTTTCACACGTGGTGTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGGACTTGTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCTCTGGGACCAATGGTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTATTTTGCTTCCACTGAGAAGTCTAACATAATAAGAGGCTGGATTTTTGGTACTACTTTAGATTCGAAGACCCAGTCCCTACTTATTGTTAATAACGCTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCATTTTTGGGTGTTTATTACCACAAAAACAACAAAAGTTGTATGGAAAGTGAGTTCAGAGTTTATTCTAGTGCGAATAATTGCACTTTTGAATATGTCTCTCAGCCTTTTCTTATGGACCTTGAAGGAAAACAGGGTAATTTCAAAAATCTTAGGGAATTTGTGTTTAAGAATATTGATGGTTATTTTAAAATATATTCTAAGCACACGCCTATTAATTTAGTGCGTGATCTCCCTCAGGGTTTTTCGGCTTTAGAACCATTGGTAGATTTGCCAATAGGTATTAACATCACTAGGTTTCAAACTTTACTTGCTTTACATAGAAGTTATTTGACTCCTGGTGATTCTTCTTCAGGTTGGACAGCTGGTGCTGCAGCTTATTATGTGGGTTATCTTCAACCTAGGACTTTTCTATTAAAATATAATGAAAATGGAACCATTACAGATGCTGTAGACTGTGCACTTGACCCTCTCTCAGAAACAAAGTGTACGTTGAAATCCTTCACTGTAGAAAAAGGAATCTATCAAACTTCTAACTTTAGAGTCCAACCAACAGAATCTATTGTTAGATTTCCTAATATTACAAACTTGTGCCCTTTTGGTGAAGTTTTTAACGCCACCAGATTTGCATCTGTTTATGCTTGGAACAGGAAGAGAATCAGCAACTGTGTTGCTGATTATTCTGTCCTATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTACTAAATTAAATGATCTCTGCTTTACTAATGTCTATGCAGATTCATTTGTAATTAGAGGTGATGAAGTCAGACAAATCGCTCCAGGGCAAACTGGAAAGATTGCTGATTATAATTATAAATTACCAGATGATTTTACAGGCTGC
GTTATAGCTTGGAATTCTAACAATCTTGATTCTAAGGTTGGTGGTAATTATAATTACCGGTATAGATTGTTTAGGAAGTCTAATCTCAAACCTTTTGAGAGAGATATTTCAACTGAAATCTATCAGGCCGGTAGCACACCTTGTAATGGTGTTGAAGGTTTTAATTGTTACTTTCCTTTACAATCATATGGTTTCCAACCCACTAATGGTGTTGGTTACCAACCATACAGAGTAGTAGTACTTTCTTTTGAACTTCTACATGCACCAGCAACTGTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTTCAATGGTTTAACAGGCACAGGTGTTCTTACTGAGTCTAACAAAAAGTTTCTGCCTTTCCAACAATTTGGCAGAGACATTGCTGACACTACTGATGCTGTCCGTGATCCACAGACACTTGAGATTCTTGACATTACACCATGTTCTTTTGGTGGTGTCAGTGTTATAACACCAGGAACAAATACTTCTAACCAGGTTGCTGTTCTTTATCAGGGTGTTAACTGCACAGAAGTCCCTGTTGCTATTCATGCAGATCAACTTACTCCTACTTGGCGTGTTTATTCTACAGGTTCTAATGTTTTTCAAACACGTGCAGGCTGTTTAATAGGGGCTGAACATGTCAACAACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACGTAGTGTAGCTAGTCAATCCATCATTGCCTACACTATGTCACTTGGTGCAGAAAATTCAGTTGCTTACTCTAATAACTCTATTGCCATACCCACAAATTTTACTATTAGTGTTACCACAGAAATTCTACCAGTGTCTATGACCAAGACATCAGTAGATTGTACAATGTACATTTGTGGTGATTCAACTGAATGCAGCAATCTTTTGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAACTGGAATAGCTGTTGAACAAGACAAAAACACCCAAGAAGTTTTTGCACAAGTCAAACAAATTTACAAAACACCACCAATTAAAGATTTTGGTGGTTTTAATTTTTCACAAATATTACCAGATCCATCAAAACCAAGCAAGAGGTCATTTATTGAAGATCTACTTTTCAACAAAGTGACACTTGCAGATGCTGGCTTCATCAAACAATATGGTGATTGCCTTGGTGATATTGCTGCTAGAGACCTCATTTGTGCACAAAAGTTTAACGGCCTTACTGTTTTGCCACCTTTGCTCACAGATGAAATGATTGCTCAATACACTTCTGCACTGTTAGCGGGTACAATCACTTCTGGTTGGACCTTTGGTGCAGGTGCTGCATTACAAATACCATTTGCTATGCAAATGGCTTATAGGTTTAATGGTATTGGAGTTACACAGAATGTTCTCTATGAGAACCAAAAATTGATTGCCAACCAATTTAATAGCGCTATTGGCAAAATTCAAGACTCACTTTCTTCCACAGCAAGTGCACTTGGAAAACTTCAAGATGTGGTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTCCAATTTTGGTGCAATTTCAAGTGTTTTAAATGATATCCTTTCACGTCTTGACAAAGTTGAGGCTGAAGTGCAAATTGATAGGTTGATCACAGGCAGACTTCAAAGTTTGCAGACATATGTGACTCAACAATTAATTAGAGCTGCAGAAATCAGAGCTTCTGCTAATCTTGCTGCTACTAAAATGTCAGAGTGTGTACTTGGACAATCAAAAAGAGTTGATTTTTGTGGAAAGGGCTATCATCTTATGTCCTTCCCTCAGTCAGCACCTCATGGTGTAGTCTTCTTGCATGTGACTTATGTCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGATGGAAAAGCACACTTTCCTCGTGAAGGTGTCTTTGTTTCAAATGGCACACACTGGTTTGTAACACAAAGGAATTTTTATGAACCACAAATCATTACTACAGACAACACATTTGTGTCTGGTAACTGTGATGTTGTAATAGGAATTGTCAACAACACAGTTTATGATCCTTTGCAACCTGAATTAGACTCATTCAAGGAGGAGTTAGATAAATATTTTAAGAATCATACATCACCAGATGTTGATTTAGGTGACATCTCTGGCATTAATGCTTCAGTTGTAAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTTGCCAAGAATTTAAATGAATCTCTCATCGATCTCCAAGAACTTGGAAAGTATGAGCAGTATATAAAATGGCCATGGTACATTTGGCTAGGTTTTATAGCTGGCTTGATTGCCATAGTAATGGTGACAATTATGCTTTGCTGTATGACCAGTTGCTGTAGTTGTCTCAAGGGCTGTTGTTCTTGTGGATCCTGCTGCAAATTTGATGAAGACGACTCTGAGCCAGTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGAATCTTCACAATTGGAACTGTAACTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTGTTCGCGCTACTGCAACGATACCGATACAAGCCTCACTCCCTTTCGGATGGCTTATTGTTGGCGTTGCACTTCTTGCTGTTTTTCATAGCGCTTCCAAAATCATAACCCTCAAAAAGAGATGGCAACTAGCACTCTCCAAGGGTGTTCACTTTGTTTGCAACTTGCTGTTGTTGTTTGTAACAGTTTACTCACACCTTTTGCTCGTTGCTGTTGGCCTTGAAGCCCCTTTTCTCTATCTTTATGCTTTAGTC
TACTTCTTGCAGAGTATAAACTTTGTAAGAATAATAATGAGGCTTTGGCTTTGCTGGAAATGCCGTTCCAAAAACCCATTACTTTATGATGCCAACTATTTTCTTTGCTGGCATACTAATTGTTACGACTATTGTATACCTTACAATAGTGTAACTTCTTCAATTGTCATTACTTCAGGTGATGGCACAACAAGTCCTATTTCTGAACATGACTACCAGATTGGTGGTTATACTGAAAAATGGGAATCTGGAGTAAAAGACTGTGTTGTATTACACAGTTACTTCACTTCAGACTATTACCAGCTGTACTCAACTCAATTGAGTACAGACACTGGTGTTGAACATGTTACCTTCTTCATCTACAATAAAATTGTTGATGAGCCTGAAGAACATGTCCAAATTCACACAATCGACGGTTCATCCGGAGTTGTTAATCCAGTAATGGAACCAATTTATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGATGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAACCTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTCTTCTAGAGTTCCTGATCTTCTGGTCTAAACGAACTAAATATTATATTAGTTTTTCTGTTTGGAACTTTAATTTTAGCCATGGCAGATTCCAACGGTACTATTACCGTTGAAGAGCTTAAAAAGCTCCTTGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTTACATGGATTTGTCTTCTACAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAATTTTTCTCTGGCTGTTATGGCCAGTAACTTTAGCTTGTTTTGTGCTTGCTGCTGTTTACAGAATAAATTGGATCACCGGTGGAATTGCTATCGCAATGGCTTGTCTTGTAGGCTTGATGTGGCTCAGCTACTTCATTGCTTCTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAAACTAACATTCTTCTCAACGTGCCACTCCATGGCACTATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGATCCTTCGTGGACATCTTCGTATTGCTGGACACCATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTCTTATTACAAATTGGGAGCTTCGCAGCGTGTAGCAGGTGACTCAGGTTTTGCTGCATACAGTCGCTACAGGATTGGCAACTATAAATTAAACACAGACCATTCCAGTAGCAGTGACAATATTGCTTTGCTTGTACAGTAAGTGACAACAGATGTTTCATCTCGTTGACTTTCAGGTTACTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATTTGGAATCTTGATTACATCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCTTTTCTTGGCACTGATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAACAAATTTGCACTGACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACTAACATGAAATTTCTTGTTTTCTTAGGAATCATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAGTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTATAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAGATGGT
ATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGACTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTCATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGCAGCAGTAGGGGAATTTCTCCTGCTAGAATGGCTGGCAATGGCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATTGAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGCATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAACAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATTGGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACACAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAAAGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATACAAAACATTTCCACCAACAGAGCCTAAAAAGGACAAAAAGAAGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAAGAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTGCTGACTCAACTCAGGCCTAAACTCATGCAGACCACACAAGGCAGATGGGCTATATAAACGTTTTCGCTTTTCCGTTTACGATATATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTACATAGCACAAGTAGATGTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAGTGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCACCGAGGCCACGCGGAGTACGATCGAGTGTACAGTGAACAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGC
서열번호: 27
> QPJ72086.1. S-단백질 표면 당단백질
MFVFLVLLPLVSIQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSCMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYRYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQGVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT
서열번호: 28
>뉴클레오캡시드 인단백질 [중증 급성 호흡기 증후군 코로나바이러스 2] (수탁번호: QIA98561)
MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
서열번호: 29
>막 당단백질 [중증 급성 호흡기 증후군 코로나바이러스 2] (수탁번호: QIA98557)
MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGD SGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ
서열번호: 30
>TLR9 작용제 올리고
AACGTTCGAG
서열번호: 31
>데옥시-7-데아자구아니신으로 변형된 올리고
TCG1AACG1TTCG1
여기서 G1은 2'-데옥시-7-데아자구아니신임
서열번호: 32
>2'-데옥시-7-데아자구아니신및 글리세롤로 변형된 올리고
TCG1AACG1TTCG1XG1CTTG1CAAG1CT
여기서 G1은 2'-데옥시-7-데아자구아니신이고 X는 글리세롤임
<110> Valneva Austria GmbH Dynavax Technologies Corporation <120> CpG-Adjuvanted SARS-CoV-2 virus vaccine <130> P6141WO00 <150> US 62983737 <151> 2020-03-01 <150> EP 20168324.0 <151> 2020-04-06 <150> EP 20202124.2 <151> 2020-10-15 <150> EP 20211936.8 <151> 2020-12-04 <150> EP 21154645.2 <151> 2021-02-01 <150> EP 21160933.4 <151> 2021-03-05 <150> US 21/20313 <151> 2021-03-01 <160> 32 <170> PatentIn version 3.5 <210> 1 <211> 29903 <212> DNA <213> SARS-CoV2 <400> 1 attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 60 gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120 cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 180 ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240 cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 300 acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 360 agactccgtg gaggaggtct tatcagaggc acgtcaacat cttaaagatg gcacttgtgg 420 cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 480 acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 540 cgaaggcatt cagtacggtc gtagtggtga gacacttggt gtccttgtcc ctcatgtggg 600 cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 660 tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 720 tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 780 actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 840 ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 900 atgcactttg tccgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 960 tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1020 gacacctttt gaaattaaat tggcaaagaa atttgacacc ttcaatgggg aatgtccaaa 1080 ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1140 gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1200 caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1260 gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1320 aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1380 atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1440 cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc 1500 ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1560 ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 1620 aatactccaa aaagagaaag tcaacatcaa tattgttggt gactttaaac ttaatgaaga 1680 gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 1740 aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac 1800 aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1860 tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 1920 tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1980 aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 2040 taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg 2100 gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2160 agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 2220 ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcaa aggaaattaa 2280 ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 2340 tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca 2400 ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2460 tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 2520 aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2580 agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 2640 aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa caaacaatac 2700 cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 2760 agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2820 acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 2880 ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc 2940 actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3000 tgagtttaaa ttggcttcac atatgtattg ttctttctac cctccagatg aggatgaaga 3060 agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3120 agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 3180 agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3240 cggcagtgag gacaatcaga caactactat tcaaacaatt gttgaggttc aacctcaatt 3300 agagatggaa cttacaccag ttgttcagac tattgaagtg aatagtttta gtggttattt 3360 aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 3420 aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc 3480 aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3540 tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 3600 acactgtctt catgttgtcg gcccaaatgt taacaaaggt gaagacattc aacttcttaa 3660 gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 3720 tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa 3780 tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3840 aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa 3900 gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3960 caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 4020 cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4080 tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttgttca 4140 agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat 4200 gctagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4260 gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc 4320 cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4380 ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg 4440 tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4500 agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 4560 gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4620 tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 4680 agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc 4740 ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4800 agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga 4860 taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4920 ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4980 aacagtagac aacattaacc tccacacgca agttgtggac atgtcaatga catatggaca 5040 acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 5100 acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5160 tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5220 cactaaaaag tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa 5280 caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5340 acctgctcta caagatgctt attacagagc aagggctggt gaagctgcta acttttgtgc 5400 acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5460 gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 5520 taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5580 cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 5640 agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5700 tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 5760 gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt 5820 acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5880 ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 5940 tgaccctaag ttggacaatt attataagaa agacaattct tatttcacag agcaaccaat 6000 tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 6060 tgataatatc aaatttgctg atgatttaaa ccagttaact ggttataaga aacctgcttc 6120 aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6180 taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 6240 gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6300 tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 6360 cgcgcaggga atggataatc ttgcctgcga agatctaaaa ccagtctctg aagaagtagt 6420 ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 6480 aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6540 cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 6600 attatctaga gtattaggtt tgaaaaccct tgctactcat ggtttagctg ctgttaatag 6660 tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6720 aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 6780 ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc 6840 atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6900 ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttataatttg 6960 gtttttacta ttaagtgttt gcctaggttc tttaatctac tcaaccgctg ctttaggtgt 7020 tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7080 ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7140 tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7200 atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat 7260 tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7320 ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 7380 acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7440 tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 7500 ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag 7560 gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7620 tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 7680 cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7740 tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7800 ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7860 taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7920 atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 7980 agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8040 tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 8100 agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac 8160 ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8220 tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 8280 ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8340 tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8400 atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc 8460 tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8520 tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 8580 gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8640 tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8700 tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc 8760 tgattttgac acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc 8820 attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 8880 gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8940 tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9000 ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata 9060 ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9120 acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 9180 tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9240 agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 9300 atctttacca ggagttttct gtggtgtaga tgctgtaaat ttacttacta atatgtttac 9360 accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9420 tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 9480 tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9540 ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9600 gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9660 cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9720 tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 9780 tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa 9840 gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9900 taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 9960 tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10020 accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10080 atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg 10140 tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10200 gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 10260 ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10320 taaggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 10380 acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc 10440 tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10500 ttttaacata gattatgact gtgtctcttt ttgttacatg caccatatgg aattaccaac 10560 tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10620 aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 10680 cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga 10740 ctttaacctt gtggctatga agtacaatta tgaacctcta acacaagacc atgttgacat 10800 actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 10860 agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10920 tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 10980 gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt 11040 agttttagtc cagagtactc aatggtcttt gttctttttt ttgtatgaaa atgccttttt 11100 accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 11160 gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11220 ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11280 tagtttgtct ggttttaagc taaaagactg tgttatgtat gcatcagctg tagtgttact 11340 aatccttatg acagcaagaa ctgtgtatga tgatggtgct aggagagtgt ggacacttat 11400 gaatgtcttg acactcgttt ataaagttta ttatggtaat gctttagatc aagccatttc 11460 catgtgggct cttataatct ctgttacttc taactactca ggtgtagtta caactgtcat 11520 gtttttggcc agaggtattg tttttatgtg tgttgagtat tgccctattt tcttcataac 11580 tggtaataca cttcagtgta taatgctagt ttattgtttc ttaggctatt tttgtacttg 11640 ttactttggc ctcttttgtt tactcaaccg ctactttaga ctgactcttg gtgtttatga 11700 ttacttagtt tctacacagg agtttagata tatgaattca cagggactac tcccacccaa 11760 gaatagcata gatgccttca aactcaacat taaattgttg ggtgttggtg gcaaaccttg 11820 tatcaaagta gccactgtac agtctaaaat gtcagatgta aagtgcacat cagtagtctt 11880 actctcagtt ttgcaacaac tcagagtaga atcatcatct aaattgtggg ctcaatgtgt 11940 ccagttacac aatgacattc tcttagctaa agatactact gaagcctttg aaaaaatggt 12000 ttcactactt tctgttttgc tttccatgca gggtgctgta gacataaaca agctttgtga 12060 agaaatgctg gacaacaggg caaccttaca agctatagcc tcagagttta gttcccttcc 12120 atcatatgca gcttttgcta ctgctcaaga agcttatgag caggctgttg ctaatggtga 12180 ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat gtggctaaat ctgaatttga 12240 ccgtgatgca gccatgcaac gtaagttgga aaagatggct gatcaagcta tgacccaaat 12300 gtataaacag gctagatctg aggacaagag ggcaaaagtt actagtgcta tgcagacaat 12360 gcttttcact atgcttagaa agttggataa tgatgcactc aacaacatta tcaacaatgc 12420 aagagatggt tgtgttccct tgaacataat acctcttaca acagcagcca aactaatggt 12480 tgtcatacca gactataaca catataaaaa tacgtgtgat ggtacaacat ttacttatgc 12540 atcagcattg tgggaaatcc aacaggttgt agatgcagat agtaaaattg ttcaacttag 12600 tgaaattagt atggacaatt cacctaattt agcatggcct cttattgtaa cagctttaag 12660 ggccaattct gctgtcaaat tacagaataa tgagcttagt cctgttgcac tacgacagat 12720 gtcttgtgct gccggtacta cacaaactgc ttgcactgat gacaatgcgt tagcttacta 12780 caacacaaca aagggaggta ggtttgtact tgcactgtta tccgatttac aggatttgaa 12840 atgggctaga ttccctaaga gtgatggaac tggtactatc tatacagaac tggaaccacc 12900 ttgtaggttt gttacagaca cacctaaagg tcctaaagtg aagtatttat actttattaa 12960 aggattaaac aacctaaata gaggtatggt acttggtagt ttagctgcca cagtacgtct 13020 acaagctggt aatgcaacag aagtgcctgc caattcaact gtattatctt tctgtgcttt 13080 tgctgtagat gctgctaaag cttacaaaga ttatctagct agtgggggac aaccaatcac 13140 taattgtgtt aagatgttgt gtacacacac tggtactggt caggcaataa cagttacacc 13200 ggaagccaat atggatcaag aatcctttgg tggtgcatcg tgttgtctgt actgccgttg 13260 ccacatagat catccaaatc ctaaaggatt ttgtgactta aaaggtaagt atgtacaaat 13320 acctacaact tgtgctaatg accctgtggg ttttacactt aaaaacacag tctgtaccgt 13380 ctgcggtatg tggaaaggtt atggctgtag ttgtgatcaa ctccgcgaac ccatgcttca 13440 gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg taagtgcagc ccgtcttaca 13500 ccgtgcggca caggcactag tactgatgtc gtatacaggg cttttgacat ctacaatgat 13560 aaagtagctg gttttgctaa attcctaaaa actaattgtt gtcgcttcca agaaaaggac 13620 gaagatgaca atttaattga ttcttacttt gtagttaaga gacacacttt ctctaactac 13680 caacatgaag aaacaattta taatttactt aaggattgtc cagctgttgc taaacatgac 13740 ttctttaagt ttagaataga cggtgacatg gtaccacata tatcacgtca acgtcttact 13800 aaatacacaa tggcagacct cgtctatgct ttaaggcatt ttgatgaagg taattgtgac 13860 acattaaaag aaatacttgt cacatacaat tgttgtgatg atgattattt caataaaaag 13920 gactggtatg attttgtaga aaacccagat atattacgcg tatacgccaa cttaggtgaa 13980 cgtgtacgcc aagctttgtt aaaaacagta caattctgtg atgccatgcg aaatgctggt 14040 attgttggtg tactgacatt agataatcaa gatctcaatg gtaactggta tgatttcggt 14100 gatttcatac aaaccacgcc aggtagtgga gttcctgttg tagattctta ttattcattg 14160 ttaatgccta tattaacctt gaccagggct ttaactgcag agtcacatgt tgacactgac 14220 ttaacaaagc cttacattaa gtgggatttg ttaaaatatg acttcacgga agagaggtta 14280 aaactctttg accgttattt taaatattgg gatcagacat accacccaaa ttgtgttaac 14340 tgtttggatg acagatgcat tctgcattgt gcaaacttta atgttttatt ctctacagtg 14400 ttcccaccta caagttttgg accactagtg agaaaaatat ttgttgatgg tgttccattt 14460 gtagtttcaa ctggatacca cttcagagag ctaggtgttg tacataatca ggatgtaaac 14520 ttacatagct ctagacttag ttttaaggaa ttacttgtgt atgctgctga ccctgctatg 14580 cacgctgctt ctggtaatct attactagat aaacgcacta cgtgcttttc agtagctgca 14640 cttactaaca atgttgcttt tcaaactgtc aaacccggta attttaacaa agacttctat 14700 gactttgctg tgtctaaggg tttctttaag gaaggaagtt ctgttgaatt aaaacacttc 14760 ttctttgctc aggatggtaa tgctgctatc agcgattatg actactatcg ttataatcta 14820 ccaacaatgt gtgatatcag acaactacta tttgtagttg aagttgttga taagtacttt 14880 gattgttacg atggtggctg tattaatgct aaccaagtca tcgtcaacaa cctagacaaa 14940 tcagctggtt ttccatttaa taaatggggt aaggctagac tttattatga ttcaatgagt 15000 tatgaggatc aagatgcact tttcgcatat acaaaacgta atgtcatccc tactataact 15060 caaatgaatc ttaagtatgc cattagtgca aagaatagag ctcgcaccgt agctggtgtc 15120 tctatctgta gtactatgac caatagacag tttcatcaaa aattattgaa atcaatagcc 15180 gccactagag gagctactgt agtaattgga acaagcaaat tctatggtgg ttggcacaac 15240 atgttaaaaa ctgtttatag tgatgtagaa aaccctcacc ttatgggttg ggattatcct 15300 aaatgtgata gagccatgcc taacatgctt agaattatgg cctcacttgt tcttgctcgc 15360 aaacatacaa cgtgttgtag cttgtcacac cgtttctata gattagctaa tgagtgtgct 15420 caagtattga gtgaaatggt catgtgtggc ggttcactat atgttaaacc aggtggaacc 15480 tcatcaggag atgccacaac tgcttatgct aatagtgttt ttaacatttg tcaagctgtc 15540 acggccaatg ttaatgcact tttatctact gatggtaaca aaattgccga taagtatgtc 15600 cgcaatttac aacacagact ttatgagtgt ctctatagaa atagagatgt tgacacagac 15660 tttgtgaatg agttttacgc atatttgcgt aaacatttct caatgatgat actctctgac 15720 gatgctgttg tgtgtttcaa tagcacttat gcatctcaag gtctagtggc tagcataaag 15780 aactttaagt cagttcttta ttatcaaaac aatgttttta tgtctgaagc aaaatgttgg 15840 actgagactg accttactaa aggacctcat gaattttgct ctcaacatac aatgctagtt 15900 aaacagggtg atgattatgt gtaccttcct tacccagatc catcaagaat cctaggggcc 15960 ggctgttttg tagatgatat cgtaaaaaca gatggtacac ttatgattga acggttcgtg 16020 tctttagcta tagatgctta cccacttact aaacatccta atcaggagta tgctgatgtc 16080 tttcatttgt acttacaata cataagaaag ctacatgatg agttaacagg acacatgtta 16140 gacatgtatt ctgttatgct tactaatgat aacacttcaa ggtattggga acctgagttt 16200 tatgaggcta tgtacacacc gcatacagtc ttacaggctg ttggggcttg tgttctttgc 16260 aattcacaga cttcattaag atgtggtgct tgcatacgta gaccattctt atgttgtaaa 16320 tgctgttacg accatgtcat atcaacatca cataaattag tcttgtctgt taatccgtat 16380 gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc aactttactt aggaggtatg 16440 agctattatt gtaaatcaca taaaccaccc attagttttc cattgtgtgc taatggacaa 16500 gtttttggtt tatataaaaa tacatgtgtt ggtagcgata atgttactga ctttaatgca 16560 attgcaacat gtgactggac aaatgctggt gattacattt tagctaacac ctgtactgaa 16620 agactcaagc tttttgcagc agaaacgctc aaagctactg aggagacatt taaactgtct 16680 tatggtattg ctactgtacg tgaagtgctg tctgacagag aattacatct ttcatgggaa 16740 gttggtaaac ctagaccacc acttaaccga aattatgtct ttactggtta tcgtgtaact 16800 aaaaacagta aagtacaaat aggagagtac acctttgaaa aaggtgacta tggtgatgct 16860 gttgtttacc gaggtacaac aacttacaaa ttaaatgttg gtgattattt tgtgctgaca 16920 tcacatacag taatgccatt aagtgcacct acactagtgc cacaagagca ctatgttaga 16980 attactggct tatacccaac actcaatatc tcagatgagt tttctagcaa tgttgcaaat 17040 tatcaaaagg ttggtatgca aaagtattct acactccagg gaccacctgg tactggtaag 17100 agtcattttg ctattggcct agctctctac tacccttctg ctcgcatagt gtatacagct 17160 tgctctcatg ccgctgttga tgcactatgt gagaaggcat taaaatattt gcctatagat 17220 aaatgtagta gaattatacc tgcacgtgct cgtgtagagt gttttgataa attcaaagtg 17280 aattcaacat tagaacagta tgtcttttgt actgtaaatg cattgcctga gacgacagca 17340 gatatagttg tctttgatga aatttcaatg gccacaaatt atgatttgag tgttgtcaat 17400 gccagattac gtgctaagca ctatgtgtac attggcgacc ctgctcaatt acctgcacca 17460 cgcacattgc taactaaggg cacactagaa ccagaatatt tcaattcagt gtgtagactt 17520 atgaaaacta taggtccaga catgttcctc ggaacttgtc ggcgttgtcc tgctgaaatt 17580 gttgacactg tgagtgcttt ggtttatgat aataagctta aagcacataa agacaaatca 17640 gctcaatgct ttaaaatgtt ttataagggt gttatcacgc atgatgtttc atctgcaatt 17700 aacaggccac aaataggcgt ggtaagagaa ttccttacac gtaaccctgc ttggagaaaa 17760 gctgtcttta tttcacctta taattcacag aatgctgtag cctcaaagat tttgggacta 17820 ccaactcaaa ctgttgattc atcacagggc tcagaatatg actatgtcat attcactcaa 17880 accactgaaa cagctcactc ttgtaatgta aacagattta atgttgctat taccagagca 17940 aaagtaggca tactttgcat aatgtctgat agagaccttt atgacaagtt gcaatttaca 18000 agtcttgaaa ttccacgtag gaatgtggca actttacaag ctgaaaatgt aacaggactc 18060 tttaaagatt gtagtaaggt aatcactggg ttacatccta cacaggcacc tacacacctc 18120 agtgttgaca ctaaattcaa aactgaaggt ttatgtgttg acatacctgg catacctaag 18180 gacatgacct atagaagact catctctatg atgggtttta aaatgaatta tcaagttaat 18240 ggttacccta acatgtttat cacccgcgaa gaagctataa gacatgtacg tgcatggatt 18300 ggcttcgatg tcgaggggtg tcatgctact agagaagctg ttggtaccaa tttaccttta 18360 cagctaggtt tttctacagg tgttaaccta gttgctgtac ctacaggtta tgttgataca 18420 cctaataata cagatttttc cagagttagt gctaaaccac cgcctggaga tcaatttaaa 18480 cacctcatac cacttatgta caaaggactt ccttggaatg tagtgcgtat aaagattgta 18540 caaatgttaa gtgacacact taaaaatctc tctgacagag tcgtatttgt cttatgggca 18600 catggctttg agttgacatc tatgaagtat tttgtgaaaa taggacctga gcgcacctgt 18660 tgtctatgtg atagacgtgc cacatgcttt tccactgctt cagacactta tgcctgttgg 18720 catcattcta ttggatttga ttacgtctat aatccgttta tgattgatgt tcaacaatgg 18780 ggttttacag gtaacctaca aagcaaccat gatctgtatt gtcaagtcca tggtaatgca 18840 catgtagcta gttgtgatgc aatcatgact aggtgtctag ctgtccacga gtgctttgtt 18900 aagcgtgttg actggactat tgaatatcct ataattggtg atgaactgaa gattaatgcg 18960 gcttgtagaa aggttcaaca catggttgtt aaagctgcat tattagcaga caaattccca 19020 gttcttcacg acattggtaa ccctaaagct attaagtgtg tacctcaagc tgatgtagaa 19080 tggaagttct atgatgcaca gccttgtagt gacaaagctt ataaaataga agaattattc 19140 tattcttatg ccacacattc tgacaaattc acagatggtg tatgcctatt ttggaattgc 19200 aatgtcgata gatatcctgc taattccatt gtttgtagat ttgacactag agtgctatct 19260 aaccttaact tgcctggttg tgatggtggc agtttgtatg taaataaaca tgcattccac 19320 acaccagctt ttgataaaag tgcttttgtt aatttaaaac aattaccatt tttctattac 19380 tctgacagtc catgtgagtc tcatggaaaa caagtagtgt cagatataga ttatgtacca 19440 ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg gtgctgtctg tagacatcat 19500 gctaatgagt acagattgta tctcgatgct tataacatga tgatctcagc tggctttagc 19560 ttgtgggttt acaaacaatt tgatacttat aacctctgga acacttttac aagacttcag 19620 agtttagaaa atgtggcttt taatgttgta aataagggac actttgatgg acaacagggt 19680 gaagtaccag tttctatcat taataacact gtttacacaa aagttgatgg tgttgatgta 19740 gaattgtttg aaaataaaac aacattacct gttaatgtag catttgagct ttgggctaag 19800 cgcaacatta aaccagtacc agaggtgaaa atactcaata atttgggtgt ggacattgct 19860 gctaatactg tgatctggga ctacaaaaga gatgctccag cacatatatc tactattggt 19920 gtttgttcta tgactgacat agccaagaaa ccaactgaaa cgatttgtgc accactcact 19980 gtcttttttg atggtagagt tgatggtcaa gtagacttat ttagaaatgc ccgtaatggt 20040 gttcttatta cagaaggtag tgttaaaggt ttacaaccat ctgtaggtcc caaacaagct 20100 agtcttaatg gagtcacatt aattggagaa gccgtaaaaa cacagttcaa ttattataag 20160 aaagttgatg gtgttgtcca acaattacct gaaacttact ttactcagag tagaaattta 20220 caagaattta aacccaggag tcaaatggaa attgatttct tagaattagc tatggatgaa 20280 ttcattgaac ggtataaatt agaaggctat gccttcgaac atatcgttta tggagatttt 20340 agtcatagtc agttaggtgg tttacatcta ctgattggac tagctaaacg ttttaaggaa 20400 tcaccttttg aattagaaga ttttattcct atggacagta cagttaaaaa ctatttcata 20460 acagatgcgc aaacaggttc atctaagtgt gtgtgttctg ttattgattt attacttgat 20520 gattttgttg aaataataaa atcccaagat ttatctgtag tttctaaggt tgtcaaagtg 20580 actattgact atacagaaat ttcatttatg ctttggtgta aagatggcca tgtagaaaca 20640 ttttacccaa aattacaatc tagtcaagcg tggcaaccgg gtgttgctat gcctaatctt 20700 tacaaaatgc aaagaatgct attagaaaag tgtgaccttc aaaattatgg tgatagtgca 20760 acattaccta aaggcataat gatgaatgtc gcaaaatata ctcaactgtg tcaatattta 20820 aacacattaa cattagctgt accctataat atgagagtta tacattttgg tgctggttct 20880 gataaaggag ttgcaccagg tacagctgtt ttaagacagt ggttgcctac gggtacgctg 20940 cttgtcgatt cagatcttaa tgactttgtc tctgatgcag attcaacttt gattggtgat 21000 tgtgcaactg tacatacagc taataaatgg gatctcatta ttagtgatat gtacgaccct 21060 aagactaaaa atgttacaaa agaaaatgac tctaaagagg gttttttcac ttacatttgt 21120 gggtttatac aacaaaagct agctcttgga ggttccgtgg ctataaagat aacagaacat 21180 tcttggaatg ctgatcttta taagctcatg ggacacttcg catggtggac agcctttgtt 21240 actaatgtga atgcgtcatc atctgaagca tttttaattg gatgtaatta tcttggcaaa 21300 ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt acatattttg gaggaataca 21360 aatccaattc agttgtcttc ctattcttta tttgacatga gtaaatttcc ccttaaatta 21420 aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca atgatatgat tttatctctt 21480 cttagtaaag gtagacttat aattagagaa aacaacagag ttgttatttc tagtgatgtt 21540 cttgttaaca actaaacgaa caatgtttgt ttttcttgtt ttattgccac tagtctctag 21600 tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac 21660 acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga 21720 cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac 21780 caatggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc 21840 ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt tagattcgaa 21900 gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt 21960 tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat 22020 ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca 22080 gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt 22140 gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt 22200 gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat 22260 taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga 22320 ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag 22380 gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact 22440 tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta 22500 tcaaacttct aactttagag tccaaccaac agaatctatt gttagatttc ctaatattac 22560 aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg 22620 gaacaggaag agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc 22680 attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac 22740 taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg 22800 gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt 22860 tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta 22920 tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta 22980 tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca 23040 atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact 23100 ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt 23160 ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac 23220 tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac 23280 tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg 23340 tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca 23400 ggatgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg 23460 gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc 23520 tgaacatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag 23580 ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat 23640 tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctaata actctattgc 23700 catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa 23760 gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt 23820 gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga 23880 acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc 23940 aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag 24000 caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt 24060 catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca 24120 aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata 24180 cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc 24240 attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca 24300 gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa 24360 aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa 24420 ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat 24480 ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat 24540 tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat 24600 tagagctgca gaaatcagag cttctgctaa tcttgctgct actaaaatgt cagagtgtgt 24660 acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc 24720 tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa 24780 gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg 24840 tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca 24900 aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt 24960 caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga 25020 taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa 25080 tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt 25140 aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc 25200 atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg tgacaattat 25260 gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg 25320 ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac 25380 ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt aactttgaag 25440 caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc aacgataccg 25500 atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct tgctgttttt 25560 cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact ctccaagggt 25620 gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca ccttttgctc 25680 gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta cttcttgcag 25740 agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg ccgttccaaa 25800 aacccattac tttatgatgc caactatttt ctttgctggc atactaattg ttacgactat 25860 tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga tggcacaaca 25920 agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg ggaatctgga 25980 gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca gctgtactca 26040 actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta caataaaatt 26100 gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acggttcatc cggagttgtt 26160 aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt gcctttgtaa 26220 gcacaagctg atgagtacga acttatgtac tcattcgttt cggaagagac aggtacgtta 26280 atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt tacactagcc 26340 atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt gagtcttgta 26400 aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag agttcctgat 26460 cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact ttaattttag 26520 ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc cttgaacaat 26580 ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa tttgcctatg 26640 ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg ttatggccag 26700 taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc accggtggaa 26760 ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc attgcttctt 26820 tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc 26880 tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa 26940 tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta ggacgctgtg 27000 acatcaagga cctgcctaaa gaaatcactg ttgctacatc acgaacgctt tcttattaca 27060 aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac agtcgctaca 27120 ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat attgctttgc 27180 ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac tatagcagag 27240 atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga ttacatcata 27300 aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc tcaattagat 27360 gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt cttggcactg 27420 ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg tacaacagta 27480 cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt tcatcctcta 27540 gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc ttgtcctgac 27600 ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact gttcatcaga 27660 caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc aatagtgttt 27720 ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca ttaattgact 27780 tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt attatctttt 27840 ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg aacatgaaat 27900 ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa tgtagtttac 27960 agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt cacttctatt 28020 ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa ttgtgcgtgg 28080 atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat acagtttcct 28140 gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta gtgcgttgtt 28200 cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat ttcatctaaa 28260 cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac 28320 gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca gtggggcgcg 28380 atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt tcaccgctct 28440 cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc caattaacac 28500 caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac gaattcgtgg 28560 tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc taggaactgg 28620 gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg ttgcaactga 28680 gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta acaatgctgc 28740 aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg cagaagggag 28800 cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa 28860 ttcaactcca ggcagcagta ggggaacttc tcctgctaga atggctggca atggcggtga 28920 tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca aaatgtctgg 28980 taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa 29040 gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag 29100 acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca gacaaggaac 29160 tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag cgttcttcgg 29220 aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct acacaggtgc 29280 catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca 29340 tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc 29400 tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc 29460 tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc 29520 aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acgttttcgc 29580 ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc 29640 acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg tgtaacatta 29700 gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac gatcgagtgt 29760 acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg taaaattaat 29820 tttagtagtg ctatccccat gtgattttaa tagcttctta ggagaatgac aaaaaaaaaa 29880 aaaaaaaaaa aaaaaaaaaa aaa 29903 <210> 2 <211> 7096 <212> PRT <213> SARS-CoV2 <400> 2 Met Glu Ser Leu Val Pro Gly Phe Asn Glu Lys Thr His Val Gln Leu 1 5 10 15 Ser Leu Pro Val Leu Gln Val Arg Asp Val Leu Val Arg Gly Phe Gly 20 25 30 Asp Ser Val Glu Glu Val Leu Ser Glu Ala Arg Gln His Leu Lys Asp 35 40 45 Gly Thr Cys Gly Leu Val Glu Val Glu Lys Gly Val Leu Pro Gln Leu 50 55 60 Glu Gln Pro Tyr Val Phe Ile Lys Arg Ser Asp Ala Arg Thr Ala Pro 65 70 75 80 His Gly His Val Met Val Glu Leu Val Ala Glu Leu Glu Gly Ile Gln 85 90 95 Tyr Gly Arg Ser Gly Glu Thr Leu Gly Val Leu Val Pro His Val Gly 100 105 110 Glu Ile Pro Val Ala Tyr Arg Lys Val Leu Leu Arg Lys Asn Gly Asn 115 120 125 Lys Gly Ala Gly Gly His Ser Tyr Gly Ala Asp Leu Lys Ser Phe Asp 130 135 140 Leu Gly Asp Glu Leu Gly Thr Asp Pro Tyr Glu Asp Phe Gln Glu Asn 145 150 155 160 Trp Asn Thr Lys His Ser Ser Gly Val Thr Arg Glu Leu Met Arg Glu 165 170 175 Leu Asn Gly Gly Ala Tyr Thr Arg Tyr Val Asp Asn Asn Phe Cys Gly 180 185 190 Pro Asp Gly Tyr Pro Leu Glu Cys Ile Lys Asp Leu Leu Ala Arg Ala 195 200 205 Gly Lys Ala Ser Cys Thr Leu Ser Glu Gln Leu Asp Phe Ile Asp Thr 210 215 220 Lys Arg Gly Val Tyr Cys Cys Arg Glu His Glu His Glu Ile Ala Trp 225 230 235 240 Tyr Thr Glu Arg Ser Glu Lys Ser Tyr Glu Leu Gln Thr Pro Phe Glu 245 250 255 Ile Lys Leu Ala Lys Lys Phe Asp Thr Phe Asn Gly Glu Cys Pro Asn 260 265 270 Phe Val Phe Pro Leu Asn Ser Ile Ile Lys Thr Ile Gln Pro Arg Val 275 280 285 Glu Lys Lys Lys Leu Asp Gly Phe Met Gly Arg Ile Arg Ser Val Tyr 290 295 300 Pro Val Ala Ser Pro Asn Glu Cys Asn Gln Met Cys Leu Ser Thr Leu 305 310 315 320 Met Lys Cys Asp His Cys Gly Glu Thr Ser Trp Gln Thr Gly Asp Phe 325 330 335 Val Lys Ala Thr Cys Glu Phe Cys Gly Thr Glu Asn Leu Thr Lys Glu 340 345 350 Gly Ala Thr Thr Cys Gly Tyr Leu Pro Gln Asn Ala Val Val Lys Ile 355 360 365 Tyr Cys Pro Ala Cys His Asn Ser Glu Val Gly Pro Glu His Ser Leu 370 375 380 Ala Glu Tyr His Asn Glu Ser Gly Leu Lys Thr Ile Leu Arg Lys Gly 385 390 395 400 Gly Arg Thr Ile Ala Phe Gly Gly Cys Val Phe Ser Tyr Val Gly Cys 405 410 415 His Asn Lys Cys Ala Tyr Trp Val Pro Arg Ala Ser Ala Asn Ile Gly 420 425 430 Cys Asn His Thr Gly Val Val Gly Glu Gly Ser Glu Gly Leu Asn Asp 435 440 445 Asn Leu Leu Glu Ile Leu Gln Lys Glu Lys Val Asn Ile Asn Ile Val 450 455 460 Gly Asp Phe Lys Leu Asn Glu Glu Ile Ala Ile Ile Leu Ala Ser Phe 465 470 475 480 Ser Ala Ser Thr Ser Ala Phe Val Glu Thr Val Lys Gly Leu Asp Tyr 485 490 495 Lys Ala Phe Lys Gln Ile Val Glu Ser Cys Gly Asn Phe Lys Val Thr 500 505 510 Lys Gly Lys Ala Lys Lys Gly Ala Trp Asn Ile Gly Glu Gln Lys Ser 515 520 525 Ile Leu Ser Pro Leu Tyr Ala Phe Ala Ser Glu Ala Ala Arg Val Val 530 535 540 Arg Ser Ile Phe Ser Arg Thr Leu Glu Thr Ala Gln Asn Ser Val Arg 545 550 555 560 Val Leu Gln Lys Ala Ala Ile Thr Ile Leu Asp Gly Ile Ser Gln Tyr 565 570 575 Ser Leu Arg Leu Ile Asp Ala Met Met Phe Thr Ser Asp Leu Ala Thr 580 585 590 Asn Asn Leu Val Val Met Ala Tyr Ile Thr Gly Gly Val Val Gln Leu 595 600 605 Thr Ser Gln Trp Leu Thr Asn Ile Phe Gly Thr Val Tyr Glu Lys Leu 610 615 620 Lys Pro Val Leu Asp Trp Leu Glu Glu Lys Phe Lys Glu Gly Val Glu 625 630 635 640 Phe Leu Arg Asp Gly Trp Glu Ile Val Lys Phe Ile Ser Thr Cys Ala 645 650 655 Cys Glu Ile Val Gly Gly Gln Ile Val Thr Cys Ala Lys Glu Ile Lys 660 665 670 Glu Ser Val Gln Thr Phe Phe Lys Leu Val Asn Lys Phe Leu Ala Leu 675 680 685 Cys Ala Asp Ser Ile Ile Ile Gly Gly Ala Lys Leu Lys Ala Leu Asn 690 695 700 Leu Gly Glu Thr Phe Val Thr His Ser Lys Gly Leu Tyr Arg Lys Cys 705 710 715 720 Val Lys Ser Arg Glu Glu Thr Gly Leu Leu Met Pro Leu Lys Ala Pro 725 730 735 Lys Glu Ile Ile Phe Leu Glu Gly Glu Thr Leu Pro Thr Glu Val Leu 740 745 750 Thr Glu Glu Val Val Leu Lys Thr Gly Asp Leu Gln Pro Leu Glu Gln 755 760 765 Pro Thr Ser Glu Ala Val Glu Ala Pro Leu Val Gly Thr Pro Val Cys 770 775 780 Ile Asn Gly Leu Met Leu Leu Glu Ile Lys Asp Thr Glu Lys Tyr Cys 785 790 795 800 Ala Leu Ala Pro Asn Met Met Val Thr Asn Asn Thr Phe Thr Leu Lys 805 810 815 Gly Gly Ala Pro Thr Lys Val Thr Phe Gly Asp Asp Thr Val Ile Glu 820 825 830 Val Gln Gly Tyr Lys Ser Val Asn Ile Thr Phe Glu Leu Asp Glu Arg 835 840 845 Ile Asp Lys Val Leu Asn Glu Lys Cys Ser Ala Tyr Thr Val Glu Leu 850 855 860 Gly Thr Glu Val Asn Glu Phe Ala Cys Val Val Ala Asp Ala Val Ile 865 870 875 880 Lys Thr Leu Gln Pro Val Ser Glu Leu Leu Thr Pro Leu Gly Ile Asp 885 890 895 Leu Asp Glu Trp Ser Met Ala Thr Tyr Tyr Leu Phe Asp Glu Ser Gly 900 905 910 Glu Phe Lys Leu Ala Ser His Met Tyr Cys Ser Phe Tyr Pro Pro Asp 915 920 925 Glu Asp Glu Glu Glu Gly Asp Cys Glu Glu Glu Glu Phe Glu Pro Ser 930 935 940 Thr Gln Tyr Glu Tyr Gly Thr Glu Asp Asp Tyr Gln Gly Lys Pro Leu 945 950 955 960 Glu Phe Gly Ala Thr Ser Ala Ala Leu Gln Pro Glu Glu Glu Gln Glu 965 970 975 Glu Asp Trp Leu Asp Asp Asp Ser Gln Gln Thr Val Gly Gln Gln Asp 980 985 990 Gly Ser Glu Asp Asn Gln Thr Thr Thr Ile Gln Thr Ile Val Glu Val 995 1000 1005 Gln Pro Gln Leu Glu Met Glu Leu Thr Pro Val Val Gln Thr Ile Glu 1010 1015 1020 Val Asn Ser Phe Ser Gly Tyr Leu Lys Leu Thr Asp Asn Val Tyr Ile 1025 1030 1035 1040 Lys Asn Ala Asp Ile Val Glu Glu Ala Lys Lys Val Lys Pro Thr Val 1045 1050 1055 Val Val Asn Ala Ala Asn Val Tyr Leu Lys His Gly Gly Gly Val Ala 1060 1065 1070 Gly Ala Leu Asn Lys Ala Thr Asn Asn Ala Met Gln Val Glu Ser Asp 1075 1080 1085 Asp Tyr Ile Ala Thr Asn Gly Pro Leu Lys Val Gly Gly Ser Cys Val 1090 1095 1100 Leu Ser Gly His Asn Leu Ala Lys His Cys Leu His Val Val Gly Pro 1105 1110 1115 1120 Asn Val Asn Lys Gly Glu Asp Ile Gln Leu Leu Lys Ser Ala Tyr Glu 1125 1130 1135 Asn Phe Asn Gln His Glu Val Leu Leu Ala Pro Leu Leu Ser Ala Gly 1140 1145 1150 Ile Phe Gly Ala Asp Pro Ile His Ser Leu Arg Val Cys Val Asp Thr 1155 1160 1165 Val Arg Thr Asn Val Tyr Leu Ala Val Phe Asp Lys Asn Leu Tyr Asp 1170 1175 1180 Lys Leu Val Ser Ser Phe Leu Glu Met Lys Ser Glu Lys Gln Val Glu 1185 1190 1195 1200 Gln Lys Ile Ala Glu Ile Pro Lys Glu Glu Val Lys Pro Phe Ile Thr 1205 1210 1215 Glu Ser Lys Pro Ser Val Glu Gln Arg Lys Gln Asp Asp Lys Lys Ile 1220 1225 1230 Lys Ala Cys Val Glu Glu Val Thr Thr Thr Leu Glu Glu Thr Lys Phe 1235 1240 1245 Leu Thr Glu Asn Leu Leu Leu Tyr Ile Asp Ile Asn Gly Asn Leu His 1250 1255 1260 Pro Asp Ser Ala Thr Leu Val Ser Asp Ile Asp Ile Thr Phe Leu Lys 1265 1270 1275 1280 Lys Asp Ala Pro Tyr Ile Val Gly Asp Val Val Gln Glu Gly Val Leu 1285 1290 1295 Thr Ala Val Val Ile Pro Thr Lys Lys Ala Gly Gly Thr Thr Glu Met 1300 1305 1310 Leu Ala Lys Ala Leu Arg Lys Val Pro Thr Asp Asn Tyr Ile Thr Thr 1315 1320 1325 Tyr Pro Gly Gln Gly Leu Asn Gly Tyr Thr Val Glu Glu Ala Lys Thr 1330 1335 1340 Val Leu Lys Lys Cys Lys Ser Ala Phe Tyr Ile Leu Pro Ser Ile Ile 1345 1350 1355 1360 Ser Asn Glu Lys Gln Glu Ile Leu Gly Thr Val Ser Trp Asn Leu Arg 1365 1370 1375 Glu Met Leu Ala His Ala Glu Glu Thr Arg Lys Leu Met Pro Val Cys 1380 1385 1390 Val Glu Thr Lys Ala Ile Val Ser Thr Ile Gln Arg Lys Tyr Lys Gly 1395 1400 1405 Ile Lys Ile Gln Glu Gly Val Val Asp Tyr Gly Ala Arg Phe Tyr Phe 1410 1415 1420 Tyr Thr Ser Lys Thr Thr Val Ala Ser Leu Ile Asn Thr Leu Asn Asp 1425 1430 1435 1440 Leu Asn Glu Thr Leu Val Thr Met Pro Leu Gly Tyr Val Thr His Gly 1445 1450 1455 Leu Asn Leu Glu Glu Ala Ala Arg Tyr Met Arg Ser Leu Lys Val Pro 1460 1465 1470 Ala Thr Val Ser Val Ser Ser Pro Asp Ala Val Thr Ala Tyr Asn Gly 1475 1480 1485 Tyr Leu Thr Ser Ser Ser Lys Thr Pro Glu Glu His Phe Ile Glu Thr 1490 1495 1500 Ile Ser Leu Ala Gly Ser Tyr Lys Asp Trp Ser Tyr Ser Gly Gln Ser 1505 1510 1515 1520 Thr Gln Leu Gly Ile Glu Phe Leu Lys Arg Gly Asp Lys Ser Val Tyr 1525 1530 1535 Tyr Thr Ser Asn Pro Thr Thr Phe His Leu Asp Gly Glu Val Ile Thr 1540 1545 1550 Phe Asp Asn Leu Lys Thr Leu Leu Ser Leu Arg Glu Val Arg Thr Ile 1555 1560 1565 Lys Val Phe Thr Thr Val Asp Asn Ile Asn Leu His Thr Gln Val Val 1570 1575 1580 Asp Met Ser Met Thr Tyr Gly Gln Gln Phe Gly Pro Thr Tyr Leu Asp 1585 1590 1595 1600 Gly Ala Asp Val Thr Lys Ile Lys Pro His Asn Ser His Glu Gly Lys 1605 1610 1615 Thr Phe Tyr Val Leu Pro Asn Asp Asp Thr Leu Arg Val Glu Ala Phe 1620 1625 1630 Glu Tyr Tyr His Thr Thr Asp Pro Ser Phe Leu Gly Arg Tyr Met Ser 1635 1640 1645 Ala Leu Asn His Thr Lys Lys Trp Lys Tyr Pro Gln Val Asn Gly Leu 1650 1655 1660 Thr Ser Ile Lys Trp Ala Asp Asn Asn Cys Tyr Leu Ala Thr Ala Leu 1665 1670 1675 1680 Leu Thr Leu Gln Gln Ile Glu Leu Lys Phe Asn Pro Pro Ala Leu Gln 1685 1690 1695 Asp Ala Tyr Tyr Arg Ala Arg Ala Gly Glu Ala Ala Asn Phe Cys Ala 1700 1705 1710 Leu Ile Leu Ala Tyr Cys Asn Lys Thr Val Gly Glu Leu Gly Asp Val 1715 1720 1725 Arg Glu Thr Met Ser Tyr Leu Phe Gln His Ala Asn Leu Asp Ser Cys 1730 1735 1740 Lys Arg Val Leu Asn Val Val Cys Lys Thr Cys Gly Gln Gln Gln Thr 1745 1750 1755 1760 Thr Leu Lys Gly Val Glu Ala Val Met Tyr Met Gly Thr Leu Ser Tyr 1765 1770 1775 Glu Gln Phe Lys Lys Gly Val Gln Ile Pro Cys Thr Cys Gly Lys Gln 1780 1785 1790 Ala Thr Lys Tyr Leu Val Gln Gln Glu Ser Pro Phe Val Met Met Ser 1795 1800 1805 Ala Pro Pro Ala Gln Tyr Glu Leu Lys His Gly Thr Phe Thr Cys Ala 1810 1815 1820 Ser Glu Tyr Thr Gly Asn Tyr Gln Cys Gly His Tyr Lys His Ile Thr 1825 1830 1835 1840 Ser Lys Glu Thr Leu Tyr Cys Ile Asp Gly Ala Leu Leu Thr Lys Ser 1845 1850 1855 Ser Glu Tyr Lys Gly Pro Ile Thr Asp Val Phe Tyr Lys Glu Asn Ser 1860 1865 1870 Tyr Thr Thr Thr Ile Lys Pro Val Thr Tyr Lys Leu Asp Gly Val Val 1875 1880 1885 Cys Thr Glu Ile Asp Pro Lys Leu Asp Asn Tyr Tyr Lys Lys Asp Asn 1890 1895 1900 Ser Tyr Phe Thr Glu Gln Pro Ile Asp Leu Val Pro Asn Gln Pro Tyr 1905 1910 1915 1920 Pro Asn Ala Ser Phe Asp Asn Phe Lys Phe Val Cys Asp Asn Ile Lys 1925 1930 1935 Phe Ala Asp Asp Leu Asn Gln Leu Thr Gly Tyr Lys Lys Pro Ala Ser 1940 1945 1950 Arg Glu Leu Lys Val Thr Phe Phe Pro Asp Leu Asn Gly Asp Val Val 1955 1960 1965 Ala Ile Asp Tyr Lys His Tyr Thr Pro Ser Phe Lys Lys Gly Ala Lys 1970 1975 1980 Leu Leu His Lys Pro Ile Val Trp His Val Asn Asn Ala Thr Asn Lys 1985 1990 1995 2000 Ala Thr Tyr Lys Pro Asn Thr Trp Cys Ile Arg Cys Leu Trp Ser Thr 2005 2010 2015 Lys Pro Val Glu Thr Ser Asn Ser Phe Asp Val Leu Lys Ser Glu Asp 2020 2025 2030 Ala Gln Gly Met Asp Asn Leu Ala Cys Glu Asp Leu Lys Pro Val Ser 2035 2040 2045 Glu Glu Val Val Glu Asn Pro Thr Ile Gln Lys Asp Val Leu Glu Cys 2050 2055 2060 Asn Val Lys Thr Thr Glu Val Val Gly Asp Ile Ile Leu Lys Pro Ala 2065 2070 2075 2080 Asn Asn Ser Leu Lys Ile Thr Glu Glu Val Gly His Thr Asp Leu Met 2085 2090 2095 Ala Ala Tyr Val Asp Asn Ser Ser Leu Thr Ile Lys Lys Pro Asn Glu 2100 2105 2110 Leu Ser Arg Val Leu Gly Leu Lys Thr Leu Ala Thr His Gly Leu Ala 2115 2120 2125 Ala Val Asn Ser Val Pro Trp Asp Thr Ile Ala Asn Tyr Ala Lys Pro 2130 2135 2140 Phe Leu Asn Lys Val Val Ser Thr Thr Thr Asn Ile Val Thr Arg Cys 2145 2150 2155 2160 Leu Asn Arg Val Cys Thr Asn Tyr Met Pro Tyr Phe Phe Thr Leu Leu 2165 2170 2175 Leu Gln Leu Cys Thr Phe Thr Arg Ser Thr Asn Ser Arg Ile Lys Ala 2180 2185 2190 Ser Met Pro Thr Thr Ile Ala Lys Asn Thr Val Lys Ser Val Gly Lys 2195 2200 2205 Phe Cys Leu Glu Ala Ser Phe Asn Tyr Leu Lys Ser Pro Asn Phe Ser 2210 2215 2220 Lys Leu Ile Asn Ile Ile Ile Trp Phe Leu Leu Leu Ser Val Cys Leu 2225 2230 2235 2240 Gly Ser Leu Ile Tyr Ser Thr Ala Ala Leu Gly Val Leu Met Ser Asn 2245 2250 2255 Leu Gly Met Pro Ser Tyr Cys Thr Gly Tyr Arg Glu Gly Tyr Leu Asn 2260 2265 2270 Ser Thr Asn Val Thr Ile Ala Thr Tyr Cys Thr Gly Ser Ile Pro Cys 2275 2280 2285 Ser Val Cys Leu Ser Gly Leu Asp Ser Leu Asp Thr Tyr Pro Ser Leu 2290 2295 2300 Glu Thr Ile Gln Ile Thr Ile Ser Ser Phe Lys Trp Asp Leu Thr Ala 2305 2310 2315 2320 Phe Gly Leu Val Ala Glu Trp Phe Leu Ala Tyr Ile Leu Phe Thr Arg 2325 2330 2335 Phe Phe Tyr Val Leu Gly Leu Ala Ala Ile Met Gln Leu Phe Phe Ser 2340 2345 2350 Tyr Phe Ala Val His Phe Ile Ser Asn Ser Trp Leu Met Trp Leu Ile 2355 2360 2365 Ile Asn Leu Val Gln Met Ala Pro Ile Ser Ala Met Val Arg Met Tyr 2370 2375 2380 Ile Phe Phe Ala Ser Phe Tyr Tyr Val Trp Lys Ser Tyr Val His Val 2385 2390 2395 2400 Val Asp Gly Cys Asn Ser Ser Thr Cys Met Met Cys Tyr Lys Arg Asn 2405 2410 2415 Arg Ala Thr Arg Val Glu Cys Thr Thr Ile Val Asn Gly Val Arg Arg 2420 2425 2430 Ser Phe Tyr Val Tyr Ala Asn Gly Gly Lys Gly Phe Cys Lys Leu His 2435 2440 2445 Asn Trp Asn Cys Val Asn Cys Asp Thr Phe Cys Ala Gly Ser Thr Phe 2450 2455 2460 Ile Ser Asp Glu Val Ala Arg Asp Leu Ser Leu Gln Phe Lys Arg Pro 2465 2470 2475 2480 Ile Asn Pro Thr Asp Gln Ser Ser Tyr Ile Val Asp Ser Val Thr Val 2485 2490 2495 Lys Asn Gly Ser Ile His Leu Tyr Phe Asp Lys Ala Gly Gln Lys Thr 2500 2505 2510 Tyr Glu Arg His Ser Leu Ser His Phe Val Asn Leu Asp Asn Leu Arg 2515 2520 2525 Ala Asn Asn Thr Lys Gly Ser Leu Pro Ile Asn Val Ile Val Phe Asp 2530 2535 2540 Gly Lys Ser Lys Cys Glu Glu Ser Ser Ala Lys Ser Ala Ser Val Tyr 2545 2550 2555 2560 Tyr Ser Gln Leu Met Cys Gln Pro Ile Leu Leu Leu Asp Gln Ala Leu 2565 2570 2575 Val Ser Asp Val Gly Asp Ser Ala Glu Val Ala Val Lys Met Phe Asp 2580 2585 2590 Ala Tyr Val Asn Thr Phe Ser Ser Thr Phe Asn Val Pro Met Glu Lys 2595 2600 2605 Leu Lys Thr Leu Val Ala Thr Ala Glu Ala Glu Leu Ala Lys Asn Val 2610 2615 2620 Ser Leu Asp Asn Val Leu Ser Thr Phe Ile Ser Ala Ala Arg Gln Gly 2625 2630 2635 2640 Phe Val Asp Ser Asp Val Glu Thr Lys Asp Val Val Glu Cys Leu Lys 2645 2650 2655 Leu Ser His Gln Ser Asp Ile Glu Val Thr Gly Asp Ser Cys Asn Asn 2660 2665 2670 Tyr Met Leu Thr Tyr Asn Lys Val Glu Asn Met Thr Pro Arg Asp Leu 2675 2680 2685 Gly Ala Cys Ile Asp Cys Ser Ala Arg His Ile Asn Ala Gln Val Ala 2690 2695 2700 Lys Ser His Asn Ile Ala Leu Ile Trp Asn Val Lys Asp Phe Met Ser 2705 2710 2715 2720 Leu Ser Glu Gln Leu Arg Lys Gln Ile Arg Ser Ala Ala Lys Lys Asn 2725 2730 2735 Asn Leu Pro Phe Lys Leu Thr Cys Ala Thr Thr Arg Gln Val Val Asn 2740 2745 2750 Val Val Thr Thr Lys Ile Ala Leu Lys Gly Gly Lys Ile Val Asn Asn 2755 2760 2765 Trp Leu Lys Gln Leu Ile Lys Val Thr Leu Val Phe Leu Phe Val Ala 2770 2775 2780 Ala Ile Phe Tyr Leu Ile Thr Pro Val His Val Met Ser Lys His Thr 2785 2790 2795 2800 Asp Phe Ser Ser Glu Ile Ile Gly Tyr Lys Ala Ile Asp Gly Gly Val 2805 2810 2815 Thr Arg Asp Ile Ala Ser Thr Asp Thr Cys Phe Ala Asn Lys His Ala 2820 2825 2830 Asp Phe Asp Thr Trp Phe Ser Gln Arg Gly Gly Ser Tyr Thr Asn Asp 2835 2840 2845 Lys Ala Cys Pro Leu Ile Ala Ala Val Ile Thr Arg Glu Val Gly Phe 2850 2855 2860 Val Val Pro Gly Leu Pro Gly Thr Ile Leu Arg Thr Thr Asn Gly Asp 2865 2870 2875 2880 Phe Leu His Phe Leu Pro Arg Val Phe Ser Ala Val Gly Asn Ile Cys 2885 2890 2895 Tyr Thr Pro Ser Lys Leu Ile Glu Tyr Thr Asp Phe Ala Thr Ser Ala 2900 2905 2910 Cys Val Leu Ala Ala Glu Cys Thr Ile Phe Lys Asp Ala Ser Gly Lys 2915 2920 2925 Pro Val Pro Tyr Cys Tyr Asp Thr Asn Val Leu Glu Gly Ser Val Ala 2930 2935 2940 Tyr Glu Ser Leu Arg Pro Asp Thr Arg Tyr Val Leu Met Asp Gly Ser 2945 2950 2955 2960 Ile Ile Gln Phe Pro Asn Thr Tyr Leu Glu Gly Ser Val Arg Val Val 2965 2970 2975 Thr Thr Phe Asp Ser Glu Tyr Cys Arg His Gly Thr Cys Glu Arg Ser 2980 2985 2990 Glu Ala Gly Val Cys Val Ser Thr Ser Gly Arg Trp Val Leu Asn Asn 2995 3000 3005 Asp Tyr Tyr Arg Ser Leu Pro Gly Val Phe Cys Gly Val Asp Ala Val 3010 3015 3020 Asn Leu Leu Thr Asn Met Phe Thr Pro Leu Ile Gln Pro Ile Gly Ala 3025 3030 3035 3040 Leu Asp Ile Ser Ala Ser Ile Val Ala Gly Gly Ile Val Ala Ile Val 3045 3050 3055 Val Thr Cys Leu Ala Tyr Tyr Phe Met Arg Phe Arg Arg Ala Phe Gly 3060 3065 3070 Glu Tyr Ser His Val Val Ala Phe Asn Thr Leu Leu Phe Leu Met Ser 3075 3080 3085 Phe Thr Val Leu Cys Leu Thr Pro Val Tyr Ser Phe Leu Pro Gly Val 3090 3095 3100 Tyr Ser Val Ile Tyr Leu Tyr Leu Thr Phe Tyr Leu Thr Asn Asp Val 3105 3110 3115 3120 Ser Phe Leu Ala His Ile Gln Trp Met Val Met Phe Thr Pro Leu Val 3125 3130 3135 Pro Phe Trp Ile Thr Ile Ala Tyr Ile Ile Cys Ile Ser Thr Lys His 3140 3145 3150 Phe Tyr Trp Phe Phe Ser Asn Tyr Leu Lys Arg Arg Val Val Phe Asn 3155 3160 3165 Gly Val Ser Phe Ser Thr Phe Glu Glu Ala Ala Leu Cys Thr Phe Leu 3170 3175 3180 Leu Asn Lys Glu Met Tyr Leu Lys Leu Arg Ser Asp Val Leu Leu Pro 3185 3190 3195 3200 Leu Thr Gln Tyr Asn Arg Tyr Leu Ala Leu Tyr Asn Lys Tyr Lys Tyr 3205 3210 3215 Phe Ser Gly Ala Met Asp Thr Thr Ser Tyr Arg Glu Ala Ala Cys Cys 3220 3225 3230 His Leu Ala Lys Ala Leu Asn Asp Phe Ser Asn Ser Gly Ser Asp Val 3235 3240 3245 Leu Tyr Gln Pro Pro Gln Thr Ser Ile Thr Ser Ala Val Leu Gln Ser 3250 3255 3260 Gly Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys Met 3265 3270 3275 3280 Val Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu Asp 3285 3290 3295 Asp Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp Met 3300 3305 3310 Leu Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His Asn 3315 3320 3325 Phe Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His Ser 3330 3335 3340 Met Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro Lys 3345 3350 3355 3360 Thr Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe Ser 3365 3370 3375 Val Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys Ala 3380 3385 3390 Met Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser Cys 3395 3400 3405 Gly Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys Tyr 3410 3415 3420 Met His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp Leu 3425 3430 3435 3440 Glu Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln Ala 3445 3450 3455 Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu Tyr 3460 3465 3470 Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr Thr 3475 3480 3485 Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu Pro 3490 3495 3500 Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln Thr 3505 3510 3515 3520 Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu Gln 3525 3530 3535 Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu Asp 3540 3545 3550 Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr Phe 3555 3560 3565 Gln Ser Ala Val Lys Arg Thr Ile Lys Gly Thr His His Trp Leu Leu 3570 3575 3580 Leu Thr Ile Leu Thr Ser Leu Leu Val Leu Val Gln Ser Thr Gln Trp 3585 3590 3595 3600 Ser Leu Phe Phe Phe Leu Tyr Glu Asn Ala Phe Leu Pro Phe Ala Met 3605 3610 3615 Gly Ile Ile Ala Met Ser Ala Phe Ala Met Met Phe Val Lys His Lys 3620 3625 3630 His Ala Phe Leu Cys Leu Phe Leu Leu Pro Ser Leu Ala Thr Val Ala 3635 3640 3645 Tyr Phe Asn Met Val Tyr Met Pro Ala Ser Trp Val Met Arg Ile Met 3650 3655 3660 Thr Trp Leu Asp Met Val Asp Thr Ser Leu Ser Gly Phe Lys Leu Lys 3665 3670 3675 3680 Asp Cys Val Met Tyr Ala Ser Ala Val Val Leu Leu Ile Leu Met Thr 3685 3690 3695 Ala Arg Thr Val Tyr Asp Asp Gly Ala Arg Arg Val Trp Thr Leu Met 3700 3705 3710 Asn Val Leu Thr Leu Val Tyr Lys Val Tyr Tyr Gly Asn Ala Leu Asp 3715 3720 3725 Gln Ala Ile Ser Met Trp Ala Leu Ile Ile Ser Val Thr Ser Asn Tyr 3730 3735 3740 Ser Gly Val Val Thr Thr Val Met Phe Leu Ala Arg Gly Ile Val Phe 3745 3750 3755 3760 Met Cys Val Glu Tyr Cys Pro Ile Phe Phe Ile Thr Gly Asn Thr Leu 3765 3770 3775 Gln Cys Ile Met Leu Val Tyr Cys Phe Leu Gly Tyr Phe Cys Thr Cys 3780 3785 3790 Tyr Phe Gly Leu Phe Cys Leu Leu Asn Arg Tyr Phe Arg Leu Thr Leu 3795 3800 3805 Gly Val Tyr Asp Tyr Leu Val Ser Thr Gln Glu Phe Arg Tyr Met Asn 3810 3815 3820 Ser Gln Gly Leu Leu Pro Pro Lys Asn Ser Ile Asp Ala Phe Lys Leu 3825 3830 3835 3840 Asn Ile Lys Leu Leu Gly Val Gly Gly Lys Pro Cys Ile Lys Val Ala 3845 3850 3855 Thr Val Gln Ser Lys Met Ser Asp Val Lys Cys Thr Ser Val Val Leu 3860 3865 3870 Leu Ser Val Leu Gln Gln Leu Arg Val Glu Ser Ser Ser Lys Leu Trp 3875 3880 3885 Ala Gln Cys Val Gln Leu His Asn Asp Ile Leu Leu Ala Lys Asp Thr 3890 3895 3900 Thr Glu Ala Phe Glu Lys Met Val Ser Leu Leu Ser Val Leu Leu Ser 3905 3910 3915 3920 Met Gln Gly Ala Val Asp Ile Asn Lys Leu Cys Glu Glu Met Leu Asp 3925 3930 3935 Asn Arg Ala Thr Leu Gln Ala Ile Ala Ser Glu Phe Ser Ser Leu Pro 3940 3945 3950 Ser Tyr Ala Ala Phe Ala Thr Ala Gln Glu Ala Tyr Glu Gln Ala Val 3955 3960 3965 Ala Asn Gly Asp Ser Glu Val Val Leu Lys Lys Leu Lys Lys Ser Leu 3970 3975 3980 Asn Val Ala Lys Ser Glu Phe Asp Arg Asp Ala Ala Met Gln Arg Lys 3985 3990 3995 4000 Leu Glu Lys Met Ala Asp Gln Ala Met Thr Gln Met Tyr Lys Gln Ala 4005 4010 4015 Arg Ser Glu Asp Lys Arg Ala Lys Val Thr Ser Ala Met Gln Thr Met 4020 4025 4030 Leu Phe Thr Met Leu Arg Lys Leu Asp Asn Asp Ala Leu Asn Asn Ile 4035 4040 4045 Ile Asn Asn Ala Arg Asp Gly Cys Val Pro Leu Asn Ile Ile Pro Leu 4050 4055 4060 Thr Thr Ala Ala Lys Leu Met Val Val Ile Pro Asp Tyr Asn Thr Tyr 4065 4070 4075 4080 Lys Asn Thr Cys Asp Gly Thr Thr Phe Thr Tyr Ala Ser Ala Leu Trp 4085 4090 4095 Glu Ile Gln Gln Val Val Asp Ala Asp Ser Lys Ile Val Gln Leu Ser 4100 4105 4110 Glu Ile Ser Met Asp Asn Ser Pro Asn Leu Ala Trp Pro Leu Ile Val 4115 4120 4125 Thr Ala Leu Arg Ala Asn Ser Ala Val Lys Leu Gln Asn Asn Glu Leu 4130 4135 4140 Ser Pro Val Ala Leu Arg Gln Met Ser Cys Ala Ala Gly Thr Thr Gln 4145 4150 4155 4160 Thr Ala Cys Thr Asp Asp Asn Ala Leu Ala Tyr Tyr Asn Thr Thr Lys 4165 4170 4175 Gly Gly Arg Phe Val Leu Ala Leu Leu Ser Asp Leu Gln Asp Leu Lys 4180 4185 4190 Trp Ala Arg Phe Pro Lys Ser Asp Gly Thr Gly Thr Ile Tyr Thr Glu 4195 4200 4205 Leu Glu Pro Pro Cys Arg Phe Val Thr Asp Thr Pro Lys Gly Pro Lys 4210 4215 4220 Val Lys Tyr Leu Tyr Phe Ile Lys Gly Leu Asn Asn Leu Asn Arg Gly 4225 4230 4235 4240 Met Val Leu Gly Ser Leu Ala Ala Thr Val Arg Leu Gln Ala Gly Asn 4245 4250 4255 Ala Thr Glu Val Pro Ala Asn Ser Thr Val Leu Ser Phe Cys Ala Phe 4260 4265 4270 Ala Val Asp Ala Ala Lys Ala Tyr Lys Asp Tyr Leu Ala Ser Gly Gly 4275 4280 4285 Gln Pro Ile Thr Asn Cys Val Lys Met Leu Cys Thr His Thr Gly Thr 4290 4295 4300 Gly Gln Ala Ile Thr Val Thr Pro Glu Ala Asn Met Asp Gln Glu Ser 4305 4310 4315 4320 Phe Gly Gly Ala Ser Cys Cys Leu Tyr Cys Arg Cys His Ile Asp His 4325 4330 4335 Pro Asn Pro Lys Gly Phe Cys Asp Leu Lys Gly Lys Tyr Val Gln Ile 4340 4345 4350 Pro Thr Thr Cys Ala Asn Asp Pro Val Gly Phe Thr Leu Lys Asn Thr 4355 4360 4365 Val Cys Thr Val Cys Gly Met Trp Lys Gly Tyr Gly Cys Ser Cys Asp 4370 4375 4380 Gln Leu Arg Glu Pro Met Leu Gln Ser Ala Asp Ala Gln Ser Phe Leu 4385 4390 4395 4400 Asn Arg Val Cys Gly Val Ser Ala Ala Arg Leu Thr Pro Cys Gly Thr 4405 4410 4415 Gly Thr Ser Thr Asp Val Val Tyr Arg Ala Phe Asp Ile Tyr Asn Asp 4420 4425 4430 Lys Val Ala Gly Phe Ala Lys Phe Leu Lys Thr Asn Cys Cys Arg Phe 4435 4440 4445 Gln Glu Lys Asp Glu Asp Asp Asn Leu Ile Asp Ser Tyr Phe Val Val 4450 4455 4460 Lys Arg His Thr Phe Ser Asn Tyr Gln His Glu Glu Thr Ile Tyr Asn 4465 4470 4475 4480 Leu Leu Lys Asp Cys Pro Ala Val Ala Lys His Asp Phe Phe Lys Phe 4485 4490 4495 Arg Ile Asp Gly Asp Met Val Pro His Ile Ser Arg Gln Arg Leu Thr 4500 4505 4510 Lys Tyr Thr Met Ala Asp Leu Val Tyr Ala Leu Arg His Phe Asp Glu 4515 4520 4525 Gly Asn Cys Asp Thr Leu Lys Glu Ile Leu Val Thr Tyr Asn Cys Cys 4530 4535 4540 Asp Asp Asp Tyr Phe Asn Lys Lys Asp Trp Tyr Asp Phe Val Glu Asn 4545 4550 4555 4560 Pro Asp Ile Leu Arg Val Tyr Ala Asn Leu Gly Glu Arg Val Arg Gln 4565 4570 4575 Ala Leu Leu Lys Thr Val Gln Phe Cys Asp Ala Met Arg Asn Ala Gly 4580 4585 4590 Ile Val Gly Val Leu Thr Leu Asp Asn Gln Asp Leu Asn Gly Asn Trp 4595 4600 4605 Tyr Asp Phe Gly Asp Phe Ile Gln Thr Thr Pro Gly Ser Gly Val Pro 4610 4615 4620 Val Val Asp Ser Tyr Tyr Ser Leu Leu Met Pro Ile Leu Thr Leu Thr 4625 4630 4635 4640 Arg Ala Leu Thr Ala Glu Ser His Val Asp Thr Asp Leu Thr Lys Pro 4645 4650 4655 Tyr Ile Lys Trp Asp Leu Leu Lys Tyr Asp Phe Thr Glu Glu Arg Leu 4660 4665 4670 Lys Leu Phe Asp Arg Tyr Phe Lys Tyr Trp Asp Gln Thr Tyr His Pro 4675 4680 4685 Asn Cys Val Asn Cys Leu Asp Asp Arg Cys Ile Leu His Cys Ala Asn 4690 4695 4700 Phe Asn Val Leu Phe Ser Thr Val Phe Pro Pro Thr Ser Phe Gly Pro 4705 4710 4715 4720 Leu Val Arg Lys Ile Phe Val Asp Gly Val Pro Phe Val Val Ser Thr 4725 4730 4735 Gly Tyr His Phe Arg Glu Leu Gly Val Val His Asn Gln Asp Val Asn 4740 4745 4750 Leu His Ser Ser Arg Leu Ser Phe Lys Glu Leu Leu Val Tyr Ala Ala 4755 4760 4765 Asp Pro Ala Met His Ala Ala Ser Gly Asn Leu Leu Leu Asp Lys Arg 4770 4775 4780 Thr Thr Cys Phe Ser Val Ala Ala Leu Thr Asn Asn Val Ala Phe Gln 4785 4790 4795 4800 Thr Val Lys Pro Gly Asn Phe Asn Lys Asp Phe Tyr Asp Phe Ala Val 4805 4810 4815 Ser Lys Gly Phe Phe Lys Glu Gly Ser Ser Val Glu Leu Lys His Phe 4820 4825 4830 Phe Phe Ala Gln Asp Gly Asn Ala Ala Ile Ser Asp Tyr Asp Tyr Tyr 4835 4840 4845 Arg Tyr Asn Leu Pro Thr Met Cys Asp Ile Arg Gln Leu Leu Phe Val 4850 4855 4860 Val Glu Val Val Asp Lys Tyr Phe Asp Cys Tyr Asp Gly Gly Cys Ile 4865 4870 4875 4880 Asn Ala Asn Gln Val Ile Val Asn Asn Leu Asp Lys Ser Ala Gly Phe 4885 4890 4895 Pro Phe Asn Lys Trp Gly Lys Ala Arg Leu Tyr Tyr Asp Ser Met Ser 4900 4905 4910 Tyr Glu Asp Gln Asp Ala Leu Phe Ala Tyr Thr Lys Arg Asn Val Ile 4915 4920 4925 Pro Thr Ile Thr Gln Met Asn Leu Lys Tyr Ala Ile Ser Ala Lys Asn 4930 4935 4940 Arg Ala Arg Thr Val Ala Gly Val Ser Ile Cys Ser Thr Met Thr Asn 4945 4950 4955 4960 Arg Gln Phe His Gln Lys Leu Leu Lys Ser Ile Ala Ala Thr Arg Gly 4965 4970 4975 Ala Thr Val Val Ile Gly Thr Ser Lys Phe Tyr Gly Gly Trp His Asn 4980 4985 4990 Met Leu Lys Thr Val Tyr Ser Asp Val Glu Asn Pro His Leu Met Gly 4995 5000 5005 Trp Asp Tyr Pro Lys Cys Asp Arg Ala Met Pro Asn Met Leu Arg Ile 5010 5015 5020 Met Ala Ser Leu Val Leu Ala Arg Lys His Thr Thr Cys Cys Ser Leu 5025 5030 5035 5040 Ser His Arg Phe Tyr Arg Leu Ala Asn Glu Cys Ala Gln Val Leu Ser 5045 5050 5055 Glu Met Val Met Cys Gly Gly Ser Leu Tyr Val Lys Pro Gly Gly Thr 5060 5065 5070 Ser Ser Gly Asp Ala Thr Thr Ala Tyr Ala Asn Ser Val Phe Asn Ile 5075 5080 5085 Cys Gln Ala Val Thr Ala Asn Val Asn Ala Leu Leu Ser Thr Asp Gly 5090 5095 5100 Asn Lys Ile Ala Asp Lys Tyr Val Arg Asn Leu Gln His Arg Leu Tyr 5105 5110 5115 5120 Glu Cys Leu Tyr Arg Asn Arg Asp Val Asp Thr Asp Phe Val Asn Glu 5125 5130 5135 Phe Tyr Ala Tyr Leu Arg Lys His Phe Ser Met Met Ile Leu Ser Asp 5140 5145 5150 Asp Ala Val Val Cys Phe Asn Ser Thr Tyr Ala Ser Gln Gly Leu Val 5155 5160 5165 Ala Ser Ile Lys Asn Phe Lys Ser Val Leu Tyr Tyr Gln Asn Asn Val 5170 5175 5180 Phe Met Ser Glu Ala Lys Cys Trp Thr Glu Thr Asp Leu Thr Lys Gly 5185 5190 5195 5200 Pro His Glu Phe Cys Ser Gln His Thr Met Leu Val Lys Gln Gly Asp 5205 5210 5215 Asp Tyr Val Tyr Leu Pro Tyr Pro Asp Pro Ser Arg Ile Leu Gly Ala 5220 5225 5230 Gly Cys Phe Val Asp Asp Ile Val Lys Thr Asp Gly Thr Leu Met Ile 5235 5240 5245 Glu Arg Phe Val Ser Leu Ala Ile Asp Ala Tyr Pro Leu Thr Lys His 5250 5255 5260 Pro Asn Gln Glu Tyr Ala Asp Val Phe His Leu Tyr Leu Gln Tyr Ile 5265 5270 5275 5280 Arg Lys Leu His Asp Glu Leu Thr Gly His Met Leu Asp Met Tyr Ser 5285 5290 5295 Val Met Leu Thr Asn Asp Asn Thr Ser Arg Tyr Trp Glu Pro Glu Phe 5300 5305 5310 Tyr Glu Ala Met Tyr Thr Pro His Thr Val Leu Gln Ala Val Gly Ala 5315 5320 5325 Cys Val Leu Cys Asn Ser Gln Thr Ser Leu Arg Cys Gly Ala Cys Ile 5330 5335 5340 Arg Arg Pro Phe Leu Cys Cys Lys Cys Cys Tyr Asp His Val Ile Ser 5345 5350 5355 5360 Thr Ser His Lys Leu Val Leu Ser Val Asn Pro Tyr Val Cys Asn Ala 5365 5370 5375 Pro Gly Cys Asp Val Thr Asp Val Thr Gln Leu Tyr Leu Gly Gly Met 5380 5385 5390 Ser Tyr Tyr Cys Lys Ser His Lys Pro Pro Ile Ser Phe Pro Leu Cys 5395 5400 5405 Ala Asn Gly Gln Val Phe Gly Leu Tyr Lys Asn Thr Cys Val Gly Ser 5410 5415 5420 Asp Asn Val Thr Asp Phe Asn Ala Ile Ala Thr Cys Asp Trp Thr Asn 5425 5430 5435 5440 Ala Gly Asp Tyr Ile Leu Ala Asn Thr Cys Thr Glu Arg Leu Lys Leu 5445 5450 5455 Phe Ala Ala Glu Thr Leu Lys Ala Thr Glu Glu Thr Phe Lys Leu Ser 5460 5465 5470 Tyr Gly Ile Ala Thr Val Arg Glu Val Leu Ser Asp Arg Glu Leu His 5475 5480 5485 Leu Ser Trp Glu Val Gly Lys Pro Arg Pro Pro Leu Asn Arg Asn Tyr 5490 5495 5500 Val Phe Thr Gly Tyr Arg Val Thr Lys Asn Ser Lys Val Gln Ile Gly 5505 5510 5515 5520 Glu Tyr Thr Phe Glu Lys Gly Asp Tyr Gly Asp Ala Val Val Tyr Arg 5525 5530 5535 Gly Thr Thr Thr Tyr Lys Leu Asn Val Gly Asp Tyr Phe Val Leu Thr 5540 5545 5550 Ser His Thr Val Met Pro Leu Ser Ala Pro Thr Leu Val Pro Gln Glu 5555 5560 5565 His Tyr Val Arg Ile Thr Gly Leu Tyr Pro Thr Leu Asn Ile Ser Asp 5570 5575 5580 Glu Phe Ser Ser Asn Val Ala Asn Tyr Gln Lys Val Gly Met Gln Lys 5585 5590 5595 5600 Tyr Ser Thr Leu Gln Gly Pro Pro Gly Thr Gly Lys Ser His Phe Ala 5605 5610 5615 Ile Gly Leu Ala Leu Tyr Tyr Pro Ser Ala Arg Ile Val Tyr Thr Ala 5620 5625 5630 Cys Ser His Ala Ala Val Asp Ala Leu Cys Glu Lys Ala Leu Lys Tyr 5635 5640 5645 Leu Pro Ile Asp Lys Cys Ser Arg Ile Ile Pro Ala Arg Ala Arg Val 5650 5655 5660 Glu Cys Phe Asp Lys Phe Lys Val Asn Ser Thr Leu Glu Gln Tyr Val 5665 5670 5675 5680 Phe Cys Thr Val Asn Ala Leu Pro Glu Thr Thr Ala Asp Ile Val Val 5685 5690 5695 Phe Asp Glu Ile Ser Met Ala Thr Asn Tyr Asp Leu Ser Val Val Asn 5700 5705 5710 Ala Arg Leu Arg Ala Lys His Tyr Val Tyr Ile Gly Asp Pro Ala Gln 5715 5720 5725 Leu Pro Ala Pro Arg Thr Leu Leu Thr Lys Gly Thr Leu Glu Pro Glu 5730 5735 5740 Tyr Phe Asn Ser Val Cys Arg Leu Met Lys Thr Ile Gly Pro Asp Met 5745 5750 5755 5760 Phe Leu Gly Thr Cys Arg Arg Cys Pro Ala Glu Ile Val Asp Thr Val 5765 5770 5775 Ser Ala Leu Val Tyr Asp Asn Lys Leu Lys Ala His Lys Asp Lys Ser 5780 5785 5790 Ala Gln Cys Phe Lys Met Phe Tyr Lys Gly Val Ile Thr His Asp Val 5795 5800 5805 Ser Ser Ala Ile Asn Arg Pro Gln Ile Gly Val Val Arg Glu Phe Leu 5810 5815 5820 Thr Arg Asn Pro Ala Trp Arg Lys Ala Val Phe Ile Ser Pro Tyr Asn 5825 5830 5835 5840 Ser Gln Asn Ala Val Ala Ser Lys Ile Leu Gly Leu Pro Thr Gln Thr 5845 5850 5855 Val Asp Ser Ser Gln Gly Ser Glu Tyr Asp Tyr Val Ile Phe Thr Gln 5860 5865 5870 Thr Thr Glu Thr Ala His Ser Cys Asn Val Asn Arg Phe Asn Val Ala 5875 5880 5885 Ile Thr Arg Ala Lys Val Gly Ile Leu Cys Ile Met Ser Asp Arg Asp 5890 5895 5900 Leu Tyr Asp Lys Leu Gln Phe Thr Ser Leu Glu Ile Pro Arg Arg Asn 5905 5910 5915 5920 Val Ala Thr Leu Gln Ala Glu Asn Val Thr Gly Leu Phe Lys Asp Cys 5925 5930 5935 Ser Lys Val Ile Thr Gly Leu His Pro Thr Gln Ala Pro Thr His Leu 5940 5945 5950 Ser Val Asp Thr Lys Phe Lys Thr Glu Gly Leu Cys Val Asp Ile Pro 5955 5960 5965 Gly Ile Pro Lys Asp Met Thr Tyr Arg Arg Leu Ile Ser Met Met Gly 5970 5975 5980 Phe Lys Met Asn Tyr Gln Val Asn Gly Tyr Pro Asn Met Phe Ile Thr 5985 5990 5995 6000 Arg Glu Glu Ala Ile Arg His Val Arg Ala Trp Ile Gly Phe Asp Val 6005 6010 6015 Glu Gly Cys His Ala Thr Arg Glu Ala Val Gly Thr Asn Leu Pro Leu 6020 6025 6030 Gln Leu Gly Phe Ser Thr Gly Val Asn Leu Val Ala Val Pro Thr Gly 6035 6040 6045 Tyr Val Asp Thr Pro Asn Asn Thr Asp Phe Ser Arg Val Ser Ala Lys 6050 6055 6060 Pro Pro Pro Gly Asp Gln Phe Lys His Leu Ile Pro Leu Met Tyr Lys 6065 6070 6075 6080 Gly Leu Pro Trp Asn Val Val Arg Ile Lys Ile Val Gln Met Leu Ser 6085 6090 6095 Asp Thr Leu Lys Asn Leu Ser Asp Arg Val Val Phe Val Leu Trp Ala 6100 6105 6110 His Gly Phe Glu Leu Thr Ser Met Lys Tyr Phe Val Lys Ile Gly Pro 6115 6120 6125 Glu Arg Thr Cys Cys Leu Cys Asp Arg Arg Ala Thr Cys Phe Ser Thr 6130 6135 6140 Ala Ser Asp Thr Tyr Ala Cys Trp His His Ser Ile Gly Phe Asp Tyr 6145 6150 6155 6160 Val Tyr Asn Pro Phe Met Ile Asp Val Gln Gln Trp Gly Phe Thr Gly 6165 6170 6175 Asn Leu Gln Ser Asn His Asp Leu Tyr Cys Gln Val His Gly Asn Ala 6180 6185 6190 His Val Ala Ser Cys Asp Ala Ile Met Thr Arg Cys Leu Ala Val His 6195 6200 6205 Glu Cys Phe Val Lys Arg Val Asp Trp Thr Ile Glu Tyr Pro Ile Ile 6210 6215 6220 Gly Asp Glu Leu Lys Ile Asn Ala Ala Cys Arg Lys Val Gln His Met 6225 6230 6235 6240 Val Val Lys Ala Ala Leu Leu Ala Asp Lys Phe Pro Val Leu His Asp 6245 6250 6255 Ile Gly Asn Pro Lys Ala Ile Lys Cys Val Pro Gln Ala Asp Val Glu 6260 6265 6270 Trp Lys Phe Tyr Asp Ala Gln Pro Cys Ser Asp Lys Ala Tyr Lys Ile 6275 6280 6285 Glu Glu Leu Phe Tyr Ser Tyr Ala Thr His Ser Asp Lys Phe Thr Asp 6290 6295 6300 Gly Val Cys Leu Phe Trp Asn Cys Asn Val Asp Arg Tyr Pro Ala Asn 6305 6310 6315 6320 Ser Ile Val Cys Arg Phe Asp Thr Arg Val Leu Ser Asn Leu Asn Leu 6325 6330 6335 Pro Gly Cys Asp Gly Gly Ser Leu Tyr Val Asn Lys His Ala Phe His 6340 6345 6350 Thr Pro Ala Phe Asp Lys Ser Ala Phe Val Asn Leu Lys Gln Leu Pro 6355 6360 6365 Phe Phe Tyr Tyr Ser Asp Ser Pro Cys Glu Ser His Gly Lys Gln Val 6370 6375 6380 Val Ser Asp Ile Asp Tyr Val Pro Leu Lys Ser Ala Thr Cys Ile Thr 6385 6390 6395 6400 Arg Cys Asn Leu Gly Gly Ala Val Cys Arg His His Ala Asn Glu Tyr 6405 6410 6415 Arg Leu Tyr Leu Asp Ala Tyr Asn Met Met Ile Ser Ala Gly Phe Ser 6420 6425 6430 Leu Trp Val Tyr Lys Gln Phe Asp Thr Tyr Asn Leu Trp Asn Thr Phe 6435 6440 6445 Thr Arg Leu Gln Ser Leu Glu Asn Val Ala Phe Asn Val Val Asn Lys 6450 6455 6460 Gly His Phe Asp Gly Gln Gln Gly Glu Val Pro Val Ser Ile Ile Asn 6465 6470 6475 6480 Asn Thr Val Tyr Thr Lys Val Asp Gly Val Asp Val Glu Leu Phe Glu 6485 6490 6495 Asn Lys Thr Thr Leu Pro Val Asn Val Ala Phe Glu Leu Trp Ala Lys 6500 6505 6510 Arg Asn Ile Lys Pro Val Pro Glu Val Lys Ile Leu Asn Asn Leu Gly 6515 6520 6525 Val Asp Ile Ala Ala Asn Thr Val Ile Trp Asp Tyr Lys Arg Asp Ala 6530 6535 6540 Pro Ala His Ile Ser Thr Ile Gly Val Cys Ser Met Thr Asp Ile Ala 6545 6550 6555 6560 Lys Lys Pro Thr Glu Thr Ile Cys Ala Pro Leu Thr Val Phe Phe Asp 6565 6570 6575 Gly Arg Val Asp Gly Gln Val Asp Leu Phe Arg Asn Ala Arg Asn Gly 6580 6585 6590 Val Leu Ile Thr Glu Gly Ser Val Lys Gly Leu Gln Pro Ser Val Gly 6595 6600 6605 Pro Lys Gln Ala Ser Leu Asn Gly Val Thr Leu Ile Gly Glu Ala Val 6610 6615 6620 Lys Thr Gln Phe Asn Tyr Tyr Lys Lys Val Asp Gly Val Val Gln Gln 6625 6630 6635 6640 Leu Pro Glu Thr Tyr Phe Thr Gln Ser Arg Asn Leu Gln Glu Phe Lys 6645 6650 6655 Pro Arg Ser Gln Met Glu Ile Asp Phe Leu Glu Leu Ala Met Asp Glu 6660 6665 6670 Phe Ile Glu Arg Tyr Lys Leu Glu Gly Tyr Ala Phe Glu His Ile Val 6675 6680 6685 Tyr Gly Asp Phe Ser His Ser Gln Leu Gly Gly Leu His Leu Leu Ile 6690 6695 6700 Gly Leu Ala Lys Arg Phe Lys Glu Ser Pro Phe Glu Leu Glu Asp Phe 6705 6710 6715 6720 Ile Pro Met Asp Ser Thr Val Lys Asn Tyr Phe Ile Thr Asp Ala Gln 6725 6730 6735 Thr Gly Ser Ser Lys Cys Val Cys Ser Val Ile Asp Leu Leu Leu Asp 6740 6745 6750 Asp Phe Val Glu Ile Ile Lys Ser Gln Asp Leu Ser Val Val Ser Lys 6755 6760 6765 Val Val Lys Val Thr Ile Asp Tyr Thr Glu Ile Ser Phe Met Leu Trp 6770 6775 6780 Cys Lys Asp Gly His Val Glu Thr Phe Tyr Pro Lys Leu Gln Ser Ser 6785 6790 6795 6800 Gln Ala Trp Gln Pro Gly Val Ala Met Pro Asn Leu Tyr Lys Met Gln 6805 6810 6815 Arg Met Leu Leu Glu Lys Cys Asp Leu Gln Asn Tyr Gly Asp Ser Ala 6820 6825 6830 Thr Leu Pro Lys Gly Ile Met Met Asn Val Ala Lys Tyr Thr Gln Leu 6835 6840 6845 Cys Gln Tyr Leu Asn Thr Leu Thr Leu Ala Val Pro Tyr Asn Met Arg 6850 6855 6860 Val Ile His Phe Gly Ala Gly Ser Asp Lys Gly Val Ala Pro Gly Thr 6865 6870 6875 6880 Ala Val Leu Arg Gln Trp Leu Pro Thr Gly Thr Leu Leu Val Asp Ser 6885 6890 6895 Asp Leu Asn Asp Phe Val Ser Asp Ala Asp Ser Thr Leu Ile Gly Asp 6900 6905 6910 Cys Ala Thr Val His Thr Ala Asn Lys Trp Asp Leu Ile Ile Ser Asp 6915 6920 6925 Met Tyr Asp Pro Lys Thr Lys Asn Val Thr Lys Glu Asn Asp Ser Lys 6930 6935 6940 Glu Gly Phe Phe Thr Tyr Ile Cys Gly Phe Ile Gln Gln Lys Leu Ala 6945 6950 6955 6960 Leu Gly Gly Ser Val Ala Ile Lys Ile Thr Glu His Ser Trp Asn Ala 6965 6970 6975 Asp Leu Tyr Lys Leu Met Gly His Phe Ala Trp Trp Thr Ala Phe Val 6980 6985 6990 Thr Asn Val Asn Ala Ser Ser Ser Glu Ala Phe Leu Ile Gly Cys Asn 6995 7000 7005 Tyr Leu Gly Lys Pro Arg Glu Gln Ile Asp Gly Tyr Val Met His Ala 7010 7015 7020 Asn Tyr Ile Phe Trp Arg Asn Thr Asn Pro Ile Gln Leu Ser Ser Tyr 7025 7030 7035 7040 Ser Leu Phe Asp Met Ser Lys Phe Pro Leu Lys Leu Arg Gly Thr Ala 7045 7050 7055 Val Met Ser Leu Lys Glu Gly Gln Ile Asn Asp Met Ile Leu Ser Leu 7060 7065 7070 Leu Ser Lys Gly Arg Leu Ile Ile Arg Glu Asn Asn Arg Val Val Ile 7075 7080 7085 Ser Ser Asp Val Leu Val Asn Asn 7090 7095 <210> 3 <211> 1273 <212> PRT <213> SARS-CoV2 <400> 3 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 225 230 235 240 Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 245 250 255 Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 260 265 270 Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 275 280 285 Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 290 295 300 Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 305 310 315 320 Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 325 330 335 Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 340 345 350 Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 355 360 365 Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 370 375 380 Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 385 390 395 400 Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 405 410 415 Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 420 425 430 Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 435 440 445 Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 450 455 460 Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 465 470 475 480 Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 485 490 495 Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 500 505 510 Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 515 520 525 Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 530 535 540 Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 545 550 555 560 Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 565 570 575 Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 580 585 590 Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 595 600 605 Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 610 615 620 His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 625 630 635 640 Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 645 650 655 Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 660 665 670 Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 675 680 685 Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 690 695 700 Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 705 710 715 720 Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 725 730 735 Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 740 745 750 Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 755 760 765 Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 770 775 780 Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 785 790 795 800 Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 805 810 815 Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 820 825 830 Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 835 840 845 Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 850 855 860 Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 865 870 875 880 Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 885 890 895 Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 900 905 910 Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 915 920 925 Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 930 935 940 Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 945 950 955 960 Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 965 970 975 Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 980 985 990 Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 995 1000 1005 Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 1010 1015 1020 Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 1025 1030 1035 1040 Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 1045 1050 1055 Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 1060 1065 1070 Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 1075 1080 1085 Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 1090 1095 1100 Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 1105 1110 1115 1120 Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 1125 1130 1135 Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 1140 1145 1150 Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 1155 1160 1165 Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 1170 1175 1180 Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 1185 1190 1195 1200 Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 1205 1210 1215 Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 1220 1225 1230 Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 1235 1240 1245 Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 1250 1255 1260 Leu Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 4 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> Artificial sequence <400> 4 tgactgtgaa cgttcgagat ga 22 <210> 5 <211> 11 <212> PRT <213> Artificial Sequence <220> <223> Artificial sequence <400> 5 Lys Leu Lys Leu Leu Leu Leu Leu Lys Leu Lys 1 5 10 <210> 6 <211> 26 <212> DNA <213> Artificial Sequence <220> <223> Artificial sequence <220> <221> misc_feature <222> (1)..(26) <223> n=deoxyinosine <400> 6 ncncncncnc ncncncncnc ncncnc 26 <210> 7 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> Artificial sequence <400> 7 tccatgacgt tcctgacgtt 20 <210> 8 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> Artificial sequence <400> 8 tcgtcgtttt gtcgttttgt cgtt 24 <210> 9 <211> 29867 <212> DNA <213> SARS-CoV2 <220> <221> misc_feature <222> (11083) <223> n is a, c, g, or t <400> 9 attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 60 gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120 cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 180 ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240 cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 300 acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 360 agactccgtg gaggaggtct tatcagaggc acgtcaacat cttaaagatg gcacttgtgg 420 cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 480 acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 540 cgaaggcatt cagtacggtc gtagtggtga gacacttggt gtccttgtcc ctcatgtggg 600 cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 660 tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 720 tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 780 actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 840 ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 900 atgcactttg tccgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 960 tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1020 gacacctttt gaaattaaat tggcaaagaa atttgacacc ttcaatgggg aatgtccaaa 1080 ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1140 gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1200 caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1260 gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1320 aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1380 atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1440 cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc 1500 ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1560 ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 1620 aatactccaa aaagagaaag tcaacatcaa tattgttggt gactttaaac ttaatgaaga 1680 gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 1740 aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac 1800 aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1860 tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 1920 tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1980 aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 2040 taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg 2100 gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2160 agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 2220 ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcta aggaaattaa 2280 ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 2340 tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca 2400 ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2460 tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 2520 aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2580 agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 2640 aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa caaacaatac 2700 cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 2760 agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2820 acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 2880 ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc 2940 actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3000 tgagtttaaa ttggcttcac atatgtattg ttctttctac cctccagatg aggatgaaga 3060 agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3120 agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 3180 agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3240 cggcagtgag gacaatcaga caactactat tcaaacaatt gttgaggttc aacctcaatt 3300 agagatggaa cttacaccag ttgttcagac tattgaagtg aatagtttta gtggttattt 3360 aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 3420 aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc 3480 aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3540 tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 3600 acactgtctt catgttgtcg gcccaaatgt taacaaaggt gaagacattc aacttcttaa 3660 gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 3720 tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa 3780 tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3840 aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa 3900 gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3960 caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 4020 cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4080 tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttgttca 4140 agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat 4200 gctagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4260 gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc 4320 cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4380 ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg 4440 tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4500 agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 4560 gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4620 tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 4680 agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc 4740 ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4800 agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga 4860 taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4920 ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4980 aacagtagac aacattaacc tccacacgca agttgtggac atgtcaatga catatggaca 5040 acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 5100 acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5160 tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5220 cactaaaaag tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa 5280 caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5340 acctgctcta caagatgctt attacagagc aagggctggt gaagctgcta acttttgtgc 5400 acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5460 gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 5520 taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5580 cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 5640 agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5700 tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 5760 gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt 5820 acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5880 ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 5940 tgaccctaag ttggacaatt attataagaa agacaattct tatttcacag agcaaccaat 6000 tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 6060 tgataatatc aaatttgctg atgatttaaa ccagttaact ggttataaga aacctgcttc 6120 aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6180 taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 6240 gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6300 tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 6360 cgcgcaggga atggataatc ttgcctgcga agatctaaaa ccagtctctg aagaagtagt 6420 ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 6480 aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6540 cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 6600 attatctaga gtattaggtt tgaaaaccct tgctactcat ggtttagctg ctgttaatag 6660 tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6720 aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 6780 ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc 6840 atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6900 ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttataatttg 6960 gtttttacta ttaagtgttt gcctaggttc tttaatctac tcaaccgctg ctttaggtgt 7020 tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7080 ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7140 tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7200 atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat 7260 tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7320 ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 7380 acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7440 tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 7500 ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag 7560 gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7620 tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 7680 cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7740 tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7800 ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7860 taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7920 atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 7980 agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8040 tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 8100 agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac 8160 ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8220 tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 8280 ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8340 tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8400 atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc 8460 tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8520 tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 8580 gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8640 tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8700 tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc 8760 tgattttgac acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc 8820 attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 8880 gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8940 tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9000 ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata 9060 ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9120 acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 9180 tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9240 agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 9300 atctttacca ggagttttct gtggtgtaga tgctgtaaat ttacttacta atatgtttac 9360 accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9420 tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 9480 tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9540 ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9600 gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9660 cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9720 tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 9780 tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa 9840 gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9900 taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 9960 tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10020 accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10080 atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg 10140 tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10200 gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 10260 ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10320 taaggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 10380 acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc 10440 tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10500 ttttaacata gattatgact gtgtctcttt ttgttacatg caccatatgg aattaccaac 10560 tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10620 aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 10680 cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga 10740 ctttaacctt gtggctatga agtacaatta tgaacctcta acacaagacc atgttgacat 10800 actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 10860 agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10920 tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 10980 gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt 11040 agttttagtc cagagtactc aatggtcttt gttctttttt ttntatgaaa atgccttttt 11100 accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 11160 gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11220 ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11280 tagtttgtct ggttttaagc taaaagactg tgttatgtat gcatcagctg tagtgttact 11340 aatccttatg acagcaagaa ctgtgtatga tgatggtgct aggagagtgt ggacacttat 11400 gaatgtcttg acactcgttt ataaagttta ttatggtaat gctttagatc aagccatttc 11460 catgtgggct cttataatct ctgttacttc taactactca ggtgtagtta caactgtcat 11520 gtttttggcc agaggtattg tttttatgtg tgttgagtat tgccctattt tcttcataac 11580 tggtaataca cttcagtgta taatgctagt ttattgtttc ttaggctatt tttgtacttg 11640 ttactttggc ctcttttgtt tactcaaccg ctactttaga ctgactcttg gtgtttatga 11700 ttacttagtt tctacacagg agtttagata tatgaattca cagggactac tcccacccaa 11760 gaatagcata gatgccttca aactcaacat taaattgttg ggtgttggtg gcaaaccttg 11820 tatcaaagta gccactgtac agtctaaaat gtcagatgta aagtgcacat cagtagtctt 11880 actctcagtt ttgcaacaac tcagagtaga atcatcatct aaattgtggg ctcaatgtgt 11940 ccagttacac aatgacattc tcttagctaa agatactact gaagcctttg aaaaaatggt 12000 ttcactactt tctgttttgc tttccatgca gggtgctgta gacataaaca agctttgtga 12060 agaaatgctg gacaacaggg caaccttaca agctatagcc tcagagttta gttcccttcc 12120 atcatatgca gcttttgcta ctgctcaaga agcttatgag caggctgttg ctaatggtga 12180 ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat gtggctaaat ctgaatttga 12240 ccgtgatgca gccatgcaac gtaagttgga aaagatggct gatcaagcta tgacccaaat 12300 gtataaacag gctagatctg aggacaagag ggcaaaagtt actagtgcta tgcagacaat 12360 gcttttcact atgcttagaa agttggataa tgatgcactc aacaacatta tcaacaatgc 12420 aagagatggt tgtgttccct tgaacataat acctcttaca acagcagcca aactaatggt 12480 tgtcatacca gactataaca catataaaaa tacgtgtgat ggtacaacat ttacttatgc 12540 atcagcattg tgggaaatcc aacaggttgt agatgcagat agtaaaattg ttcaacttag 12600 tgaaattagt atggacaatt cacctaattt agcatggcct cttattgtaa cagctttaag 12660 ggccaattct gctgtcaaat tacagaataa tgagcttagt cctgttgcac tacgacagat 12720 gtcttgtgct gccggtacta cacaaactgc ttgcactgat gacaatgcgt tagcttacta 12780 caacacaaca aagggaggta ggtttgtact tgcactgtta tccgatttac aggatttgaa 12840 atgggctaga ttccctaaga gtgatggaac tggtactatc tatacagaac tggaaccacc 12900 ttgtaggttt gttacagaca cacctaaagg tcctaaagtg aagtatttat actttattaa 12960 aggattaaac aacctaaata gaggtatggt acttggtagt ttagctgcca cagtacgtct 13020 acaagctggt aatgcaacag aagtgcctgc caattcaact gtattatctt tctgtgcttt 13080 tgctgtagat gctgctaaag cttacaaaga ttatctagct agtgggggac aaccaatcac 13140 taattgtgtt aagatgttgt gtacacacac tggtactggt caggcaataa cagttacacc 13200 ggaagccaat atggatcaag aatcctttgg tggtgcatcg tgttgtctgt actgccgttg 13260 ccacatagat catccaaatc ctaaaggatt ttgtgactta aaaggtaagt atgtacaaat 13320 acctacaact tgtgctaatg accctgtggg ttttacactt aaaaacacag tctgtaccgt 13380 ctgcggtatg tggaaaggtt atggctgtag ttgtgatcaa ctccgcgaac ccatgcttca 13440 gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg taagtgcagc ccgtcttaca 13500 ccgtgcggca caggcactag tactgatgtc gtatacaggg cttttgacat ctacaatgat 13560 aaagtagctg gttttgctaa attcctaaaa actaattgtt gtcgcttcca agaaaaggac 13620 gaagatgaca atttaattga ttcttacttt gtagttaaga gacacacttt ctctaactac 13680 caacatgaag aaacaattta taatttactt aaggattgtc cagctgttgc taaacatgac 13740 ttctttaagt ttagaataga cggtgacatg gtaccacata tatcacgtca acgtcttact 13800 aaatacacaa tggcagacct cgtctatgct ttaaggcatt ttgatgaagg taattgtgac 13860 acattaaaag aaatacttgt cacatacaat tgttgtgatg atgattattt caataaaaag 13920 gactggtatg attttgtaga aaacccagat atattacgcg tatacgccaa cttaggtgaa 13980 cgtgtacgcc aagctttgtt aaaaacagta caattctgtg atgccatgcg aaatgctggt 14040 attgttggtg tactgacatt agataatcaa gatctcaatg gtaactggta tgatttcggt 14100 gatttcatac aaaccacgcc aggtagtgga gttcctgttg tagattctta ttattcattg 14160 ttaatgccta tattaacctt gaccagggct ttaactgcag agtcacatgt tgacactgac 14220 ttaacaaagc cttacattaa gtgggatttg ttaaaatatg acttcacgga agagaggtta 14280 aaactctttg accgttattt taaatattgg gatcagacat accacccaaa ttgtgttaac 14340 tgtttggatg acagatgcat tctgcattgt gcaaacttta atgttttatt ctctacagtg 14400 ttcccaccta caagttttgg accactagtg agaaaaatat ttgttgatgg tgttccattt 14460 gtagtttcaa ctggatacca cttcagagag ctaggtgttg tacataatca ggatgtaaac 14520 ttacatagct ctagacttag ttttaaggaa ttacttgtgt atgctgctga ccctgctatg 14580 cacgctgctt ctggtaatct attactagat aaacgcacta cgtgcttttc agtagctgca 14640 cttactaaca atgttgcttt tcaaactgtc aaacccggta attttaacaa agacttctat 14700 gactttgctg tgtctaaggg tttctttaag gaaggaagtt ctgttgaatt aaaacacttc 14760 ttctttgctc aggatggtaa tgctgctatc agcgattatg actactatcg ttataatcta 14820 ccaacaatgt gtgatatcag acaactacta tttgtagttg aagttgttga taagtacttt 14880 gattgttacg atggtggctg tattaatgct aaccaagtca tcgtcaacaa cctagacaaa 14940 tcagctggtt ttccatttaa taaatggggt aaggctagac tttattatga ttcaatgagt 15000 tatgaggatc aagatgcact tttcgcatat acaaaacgta atgtcatccc tactataact 15060 caaatgaatc ttaagtatgc cattagtgca aagaatagag ctcgcaccgt agctggtgtc 15120 tctatctgta gtactatgac caatagacag tttcatcaaa aattattgaa atcaatagcc 15180 gccactagag gagctactgt agtaattgga acaagcaaat tctatggtgg ttggcacaac 15240 atgttaaaaa ctgtttatag tgatgtagaa aaccctcacc ttatgggttg ggattatcct 15300 aaatgtgata gagccatgcc taacatgctt agaattatgg cctcacttgt tcttgctcgc 15360 aaacatacaa cgtgttgtag cttgtcacac cgtttctata gattagctaa tgagtgtgct 15420 caagtattga gtgaaatggt catgtgtggc ggttcactat atgttaaacc aggtggaacc 15480 tcatcaggag atgccacaac tgcttatgct aatagtgttt ttaacatttg tcaagctgtc 15540 acggccaatg ttaatgcact tttatctact gatggtaaca aaattgccga taagtatgtc 15600 cgcaatttac aacacagact ttatgagtgt ctctatagaa atagagatgt tgacacagac 15660 tttgtgaatg agttttacgc atatttgcgt aaacatttct caatgatgat actctctgac 15720 gatgctgttg tgtgtttcaa tagcacttat gcatctcaag gtctagtggc tagcataaag 15780 aactttaagt cagttcttta ttatcaaaac aatgttttta tgtctgaagc aaaatgttgg 15840 actgagactg accttactaa aggacctcat gaattttgct ctcaacatac aatgctagtt 15900 aaacagggtg atgattatgt gtaccttcct tacccagatc catcaagaat cctaggggcc 15960 ggctgttttg tagatgatat cgtaaaaaca gatggtacac ttatgattga acggttcgtg 16020 tctttagcta tagatgctta cccacttact aaacatccta atcaggagta tgctgatgtc 16080 tttcatttgt acttacaata cataagaaag ctacatgatg agttaacagg acacatgtta 16140 gacatgtatt ctgttatgct tactaatgat aacacttcaa ggtattggga acctgagttt 16200 tatgaggcta tgtacacacc gcatacagtc ttacaggctg ttggggcttg tgttctttgc 16260 aattcacaga cttcattaag atgtggtgct tgcatacgta gaccattctt atgttgtaaa 16320 tgctgttacg accatgtcat atcaacatca cataaattag tcttgtctgt taatccgtat 16380 gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc aactttactt aggaggtatg 16440 agctattatt gtaaatcaca taaaccaccc attagttttc cattgtgtgc taatggacaa 16500 gtttttggtt tatataaaaa tacatgtgtt ggtagcgata atgttactga ctttaatgca 16560 attgcaacat gtgactggac aaatgctggt gattacattt tagctaacac ctgtactgaa 16620 agactcaagc tttttgcagc agaaacgctc aaagctactg aggagacatt taaactgtct 16680 tatggtattg ctactgtacg tgaagtgctg tctgacagag aattacatct ttcatgggaa 16740 gttggtaaac ctagaccacc acttaaccga aattatgtct ttactggtta tcgtgtaact 16800 aaaaacagta aagtacaaat aggagagtac acctttgaaa aaggtgacta tggtgatgct 16860 gttgtttacc gaggtacaac aacttacaaa ttaaatgttg gtgattattt tgtgctgaca 16920 tcacatacag taatgccatt aagtgcacct acactagtgc cacaagagca ctatgttaga 16980 attactggct tatacccaac actcaatatc tcagatgagt tttctagcaa tgttgcaaat 17040 tatcaaaagg ttggtatgca aaagtattct acactccagg gaccacctgg tactggtaag 17100 agtcattttg ctattggcct agctctctac tacccttctg ctcgcatagt gtatacagct 17160 tgctctcatg ccgctgttga tgcactatgt gagaaggcat taaaatattt gcctatagat 17220 aaatgtagta gaattatacc tgcacgtgct cgtgtagagt gttttgataa attcaaagtg 17280 aattcaacat tagaacagta tgtcttttgt actgtaaatg cattgcctga gacgacagca 17340 gatatagttg tctttgatga aatttcaatg gccacaaatt atgatttgag tgttgtcaat 17400 gccagattac gtgctaagca ctatgtgtac attggcgacc ctgctcaatt acctgcacca 17460 cgcacattgc taactaaggg cacactagaa ccagaatatt tcaattcagt gtgtagactt 17520 atgaaaacta taggtccaga catgttcctc ggaacttgtc ggcgttgtcc tgctgaaatt 17580 gttgacactg tgagtgcttt ggtttatgat aataagctta aagcacataa agacaaatca 17640 gctcaatgct ttaaaatgtt ttataagggt gttatcacgc atgatgtttc atctgcaatt 17700 aacaggccac aaataggcgt ggtaagagaa ttccttacac gtaaccctgc ttggagaaaa 17760 gctgtcttta tttcacctta taattcacag aatgctgtag cctcaaagat tttgggacta 17820 ccaactcaaa ctgttgattc atcacagggc tcagaatatg actatgtcat attcactcaa 17880 accactgaaa cagctcactc ttgtaatgta aacagattta atgttgctat taccagagca 17940 aaagtaggca tactttgcat aatgtctgat agagaccttt atgacaagtt gcaatttaca 18000 agtcttgaaa ttccacgtag gaatgtggca actttacaag ctgaaaatgt aacaggactc 18060 tttaaagatt gtagtaaggt aatcactggg ttacatccta cacaggcacc tacacacctc 18120 agtgttgaca ctaaattcaa aactgaaggt ttatgtgttg acatacctgg catacctaag 18180 gacatgacct atagaagact catctctatg atgggtttta aaatgaatta tcaagttaat 18240 ggttacccta acatgtttat cacccgcgaa gaagctataa gacatgtacg tgcatggatt 18300 ggcttcgatg tcgaggggtg tcatgctact agagaagctg ttggtaccaa tttaccttta 18360 cagctaggtt tttctacagg tgttaaccta gttgctgtac ctacaggtta tgttgataca 18420 cctaataata cagatttttc cagagttagt gctaaaccac cgcctggaga tcaatttaaa 18480 cacctcatac cacttatgta caaaggactt ccttggaatg tagtgcgtat aaagattgta 18540 caaatgttaa gtgacacact taaaaatctc tctgacagag tcgtatttgt cttatgggca 18600 catggctttg agttgacatc tatgaagtat tttgtgaaaa taggacctga gcgcacctgt 18660 tgtctatgtg atagacgtgc cacatgcttt tccactgctt cagacactta tgcctgttgg 18720 catcattcta ttggatttga ttacgtctat aatccgttta tgattgatgt tcaacaatgg 18780 ggttttacag gtaacctaca aagcaaccat gatctgtatt gtcaagtcca tggtaatgca 18840 catgtagcta gttgtgatgc aatcatgact aggtgtctag ctgtccacga gtgctttgtt 18900 aagcgtgttg actggactat tgaatatcct ataattggtg atgaactgaa gattaatgcg 18960 gcttgtagaa aggttcaaca catggttgtt aaagctgcat tattagcaga caaattccca 19020 gttcttcacg acattggtaa ccctaaagct attaagtgtg tacctcaagc tgatgtagaa 19080 tggaagttct atgatgcaca gccttgtagt gacaaagctt ataaaataga agaattattc 19140 tattcttatg ccacacattc tgacaaattc acagatggtg tatgcctatt ttggaattgc 19200 aatgtcgata gatatcctgc taattccatt gtttgtagat ttgacactag agtgctatct 19260 aaccttaact tgcctggttg tgatggtggc agtttgtatg taaataaaca tgcattccac 19320 acaccagctt ttgataaaag tgcttttgtt aatttaaaac aattaccatt tttctattac 19380 tctgacagtc catgtgagtc tcatggaaaa caagtagtgt cagatataga ttatgtacca 19440 ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg gtgctgtctg tagacatcat 19500 gctaatgagt acagattgta tctcgatgct tataacatga tgatctcagc tggctttagc 19560 ttgtgggttt acaaacaatt tgatacttat aacctctgga acacttttac aagacttcag 19620 agtttagaaa atgtggcttt taatgttgta aataagggac actttgatgg acaacagggt 19680 gaagtaccag tttctatcat taataacact gtttacacaa aagttgatgg tgttgatgta 19740 gaattgtttg aaaataaaac aacattacct gttaatgtag catttgagct ttgggctaag 19800 cgcaacatta aaccagtacc agaggtgaaa atactcaata atttgggtgt ggacattgct 19860 gctaatactg tgatctggga ctacaaaaga gatgctccag cacatatatc tactattggt 19920 gtttgttcta tgactgacat agccaagaaa ccaactgaaa cgatttgtgc accactcact 19980 gtcttttttg atggtagagt tgatggtcaa gtagacttat ttagaaatgc ccgtaatggt 20040 gttcttatta cagaaggtag tgttaaaggt ttacaaccat ctgtaggtcc caaacaagct 20100 agtcttaatg gagtcacatt aattggagaa gccgtaaaaa cacagttcaa ttattataag 20160 aaagttgatg gtgttgtcca acaattacct gaaacttact ttactcagag tagaaattta 20220 caagaattta aacccaggag tcaaatggaa attgatttct tagaattagc tatggatgaa 20280 ttcattgaac ggtataaatt agaaggctat gccttcgaac atatcgttta tggagatttt 20340 agtcatagtc agttaggtgg tttacatcta ctgattggac tagctaaacg ttttaaggaa 20400 tcaccttttg aattagaaga ttttattcct atggacagta cagttaaaaa ctatttcata 20460 acagatgcgc aaacaggttc atctaagtgt gtgtgttctg ttattgattt attacttgat 20520 gattttgttg aaataataaa atcccaagat ttatctgtag tttctaaggt tgtcaaagtg 20580 actattgact atacagaaat ttcatttatg ctttggtgta aagatggcca tgtagaaaca 20640 ttttacccaa aattacaatc tagtcaagcg tggcaaccgg gtgttgctat gcctaatctt 20700 tacaaaatgc aaagaatgct attagaaaag tgtgaccttc aaaattatgg tgatagtgca 20760 acattaccta aaggcataat gatgaatgtc gcaaaatata ctcaactgtg tcaatattta 20820 aacacattaa cattagctgt accctataat atgagagtta tacattttgg tgctggttct 20880 gataaaggag ttgcaccagg tacagctgtt ttaagacagt ggttgcctac gggtacgctg 20940 cttgtcgatt cagatcttaa tgactttgtc tctgatgcag attcaacttt gattggtgat 21000 tgtgcaactg tacatacagc taataaatgg gatctcatta ttagtgatat gtacgaccct 21060 aagactaaaa atgttacaaa agaaaatgac tctaaagagg gttttttcac ttacatttgt 21120 gggtttatac aacaaaagct agctcttgga ggttccgtgg ctataaagat aacagaacat 21180 tcttggaatg ctgatcttta taagctcatg ggacacttcg catggtggac agcctttgtt 21240 actaatgtga atgcgtcatc atctgaagca tttttaattg gatgtaatta tcttggcaaa 21300 ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt acatattttg gaggaataca 21360 aatccaattc agttgtcttc ctattcttta tttgacatga gtaaatttcc ccttaaatta 21420 aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca atgatatgat tttatctctt 21480 cttagtaaag gtagacttat aattagagaa aacaacagag ttgttatttc tagtgatgtt 21540 cttgttaaca actaaacgaa caatgtttgt ttttcttgtt ttattgccac tagtctctag 21600 tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac 21660 acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga 21720 cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac 21780 caatggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc 21840 ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt tagattcgaa 21900 gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt 21960 tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat 22020 ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca 22080 gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt 22140 gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt 22200 gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat 22260 taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga 22320 ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag 22380 gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact 22440 tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta 22500 tcaaacttct aactttagag tccaaccaac agaatctatt gttagatttc ctaatattac 22560 aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg 22620 gaacaggaag agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc 22680 attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac 22740 taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg 22800 gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt 22860 tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta 22920 tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta 22980 tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca 23040 atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact 23100 ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt 23160 ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac 23220 tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac 23280 tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg 23340 tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca 23400 ggatgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg 23460 gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc 23520 tgaacatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag 23580 ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat 23640 tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctaata actctattgc 23700 catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa 23760 gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt 23820 gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga 23880 acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc 23940 aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag 24000 caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt 24060 catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca 24120 aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata 24180 cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc 24240 attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca 24300 gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa 24360 aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa 24420 ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat 24480 ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat 24540 tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat 24600 tagagctgca gaaatcagag cttctgctaa tcttgctgct actaaaatgt cagagtgtgt 24660 acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc 24720 tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa 24780 gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg 24840 tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca 24900 aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt 24960 caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga 25020 taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa 25080 tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt 25140 aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc 25200 atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg tgacaattat 25260 gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg 25320 ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac 25380 ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt aactttgaag 25440 caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc aacgataccg 25500 atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct tgctgttttt 25560 cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact ctccaagggt 25620 gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca ccttttgctc 25680 gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta cttcttgcag 25740 agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg ccgttccaaa 25800 aacccattac tttatgatgc caactatttt ctttgctggc atactaattg ttacgactat 25860 tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga tggcacaaca 25920 agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg ggaatctgga 25980 gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca gctgtactca 26040 actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta caataaaatt 26100 gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acgtttcatc cggagttgtt 26160 aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt gcctttgtaa 26220 gcacaagctg atgagtacga acttatgtac tcattcgttt cggaagagac aggtacgtta 26280 atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt tacactagcc 26340 atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt gagtcttgta 26400 aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag agttcctgat 26460 cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact ttaattttag 26520 ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc cttgaacaat 26580 ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa tttgcctatg 26640 ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg ttatggccag 26700 taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc accggtggaa 26760 ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc attgcttctt 26820 tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc 26880 tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa 26940 tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta ggacgctgtg 27000 acatcaagga cctgcctaaa gaaatcactg ttgctacatc acgaacgctt tcttattaca 27060 aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac agtcgctaca 27120 ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat attgctttgc 27180 ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac tatagcagag 27240 atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga ttacatcata 27300 aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc tcaattagat 27360 gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt cttggcactg 27420 ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg tacaacagta 27480 cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt tcatcctcta 27540 gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc ttgtcctgac 27600 ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact gttcatcaga 27660 caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc aatagtgttt 27720 ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca ttaattgact 27780 tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt attatctttt 27840 ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg aacatgaaat 27900 ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa tgtagtttac 27960 agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt cacttctatt 28020 ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa ttgtgcgtgg 28080 atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat acagtttcct 28140 gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta gtgcgttgtt 28200 cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat ttcatctaaa 28260 cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac 28320 gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca gtggggcgcg 28380 atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt tcaccgctct 28440 cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc caattaacac 28500 caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac gaattcgtgg 28560 tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc taggaactgg 28620 gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg ttgcaactga 28680 gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta acaatgctgc 28740 aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg cagaagggag 28800 cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa 28860 ttcaactcca ggcagcagta ggggaacttc tcctgctaga atggctggca atggcggtga 28920 tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca aaatgtctgg 28980 taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa 29040 gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag 29100 acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca gacaaggaac 29160 tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag cgttcttcgg 29220 aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct acacaggtgc 29280 catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca 29340 tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc 29400 tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc 29460 tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc 29520 aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acgttttcgc 29580 ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc 29640 acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg tgtaacatta 29700 gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac gatcgagtgt 29760 acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg taaaattaat 29820 tttagtagtg ctatccccat gtgattttaa tagcttctta ggagaat 29867 <210> 10 <211> 7062 <212> PRT <213> SARS-CoV2 <220> <221> MISC_FEATURE <222> (3606) <223> Xaa can be any naturally occurring amino acid <400> 10 Met Glu Ser Leu Val Pro Gly Phe Asn Glu Lys Thr His Val Gln Leu 1 5 10 15 Ser Leu Pro Val Leu Gln Val Arg Asp Val Leu Val Arg Gly Phe Gly 20 25 30 Asp Ser Val Glu Glu Val Leu Ser Glu Ala Arg Gln His Leu Lys Asp 35 40 45 Gly Thr Cys Gly Leu Val Glu Val Glu Lys Gly Val Leu Pro Gln Leu 50 55 60 Glu Gln Pro Tyr Val Phe Ile Lys Arg Ser Asp Ala Arg Thr Ala Pro 65 70 75 80 His Gly His Val Met Val Glu Leu Val Ala Glu Leu Glu Gly Ile Gln 85 90 95 Tyr Gly Arg Ser Gly Glu Thr Leu Gly Val Leu Val Pro His Val Gly 100 105 110 Glu Ile Pro Val Ala Tyr Arg Lys Val Leu Leu Arg Lys Asn Gly Asn 115 120 125 Lys Gly Ala Gly Gly His Ser Tyr Gly Ala Asp Leu Lys Ser Phe Asp 130 135 140 Leu Gly Asp Glu Leu Gly Thr Asp Pro Tyr Glu Asp Phe Gln Glu Asn 145 150 155 160 Trp Asn Thr Lys His Ser Ser Gly Val Thr Arg Glu Leu Met Arg Glu 165 170 175 Leu Asn Gly Gly Ala Tyr Thr Arg Tyr Val Asp Asn Asn Phe Cys Gly 180 185 190 Pro Asp Gly Tyr Pro Leu Glu Cys Ile Lys Asp Leu Leu Ala Arg Ala 195 200 205 Gly Lys Ala Ser Cys Thr Leu Ser Glu Gln Leu Asp Phe Ile Asp Thr 210 215 220 Lys Arg Gly Val Tyr Cys Cys Arg Glu His Glu His Glu Ile Ala Trp 225 230 235 240 Tyr Thr Glu Arg Ser Glu Lys Ser Tyr Glu Leu Gln Thr Pro Phe Glu 245 250 255 Ile Lys Leu Ala Lys Lys Phe Asp Thr Phe Asn Gly Glu Cys Pro Asn 260 265 270 Phe Val Phe Pro Leu Asn Ser Ile Ile Lys Thr Ile Gln Pro Arg Val 275 280 285 Glu Lys Lys Lys Leu Asp Gly Phe Met Gly Arg Ile Arg Ser Val Tyr 290 295 300 Pro Val Ala Ser Pro Asn Glu Cys Asn Gln Met Cys Leu Ser Thr Leu 305 310 315 320 Met Lys Cys Asp His Cys Gly Glu Thr Ser Trp Gln Thr Gly Asp Phe 325 330 335 Val Lys Ala Thr Cys Glu Phe Cys Gly Thr Glu Asn Leu Thr Lys Glu 340 345 350 Gly Ala Thr Thr Cys Gly Tyr Leu Pro Gln Asn Ala Val Val Lys Ile 355 360 365 Tyr Cys Pro Ala Cys His Asn Ser Glu Val Gly Pro Glu His Ser Leu 370 375 380 Ala Glu Tyr His Asn Glu Ser Gly Leu Lys Thr Ile Leu Arg Lys Gly 385 390 395 400 Gly Arg Thr Ile Ala Phe Gly Gly Cys Val Phe Ser Tyr Val Gly Cys 405 410 415 His Asn Lys Cys Ala Tyr Trp Val Pro Arg Ala Ser Ala Asn Ile Gly 420 425 430 Cys Asn His Thr Gly Val Val Gly Glu Gly Ser Glu Gly Leu Asn Asp 435 440 445 Asn Leu Leu Glu Ile Leu Gln Lys Glu Lys Val Asn Ile Asn Ile Val 450 455 460 Gly Asp Phe Lys Leu Asn Glu Glu Ile Ala Ile Ile Leu Ala Ser Phe 465 470 475 480 Ser Ala Ser Thr Ser Ala Phe Val Glu Thr Val Lys Gly Leu Asp Tyr 485 490 495 Lys Ala Phe Lys Gln Ile Val Glu Ser Cys Gly Asn Phe Lys Val Thr 500 505 510 Lys Gly Lys Ala Lys Lys Gly Ala Trp Asn Ile Gly Glu Gln Lys Ser 515 520 525 Ile Leu Ser Pro Leu Tyr Ala Phe Ala Ser Glu Ala Ala Arg Val Val 530 535 540 Arg Ser Ile Phe Ser Arg Thr Leu Glu Thr Ala Gln Asn Ser Val Arg 545 550 555 560 Val Leu Gln Lys Ala Ala Ile Thr Ile Leu Asp Gly Ile Ser Gln Tyr 565 570 575 Ser Leu Arg Leu Ile Asp Ala Met Met Phe Thr Ser Asp Leu Ala Thr 580 585 590 Asn Asn Leu Val Val Met Ala Tyr Ile Thr Gly Gly Val Val Gln Leu 595 600 605 Thr Ser Gln Trp Leu Thr Asn Ile Phe Gly Thr Val Tyr Glu Lys Leu 610 615 620 Lys Pro Val Leu Asp Trp Leu Glu Glu Lys Phe Lys Glu Gly Val Glu 625 630 635 640 Phe Leu Arg Asp Gly Trp Glu Ile Val Lys Phe Ile Ser Thr Cys Ala 645 650 655 Cys Glu Ile Val Gly Gly Gln Ile Val Thr Cys Ala Lys Glu Ile Lys 660 665 670 Glu Ser Val Gln Thr Phe Phe Lys Leu Val Asn Lys Phe Leu Ala Leu 675 680 685 Cys Ala Asp Ser Ile Ile Ile Gly Gly Ala Lys Leu Lys Ala Leu Asn 690 695 700 Leu Gly Glu Thr Phe Val Thr His Ser Lys Gly Leu Tyr Arg Lys Cys 705 710 715 720 Val Lys Ser Arg Glu Glu Thr Gly Leu Leu Met Pro Leu Lys Ala Pro 725 730 735 Lys Glu Ile Ile Phe Leu Glu Gly Glu Thr Leu Pro Thr Glu Val Leu 740 745 750 Thr Glu Glu Val Val Leu Lys Thr Gly Asp Leu Gln Pro Leu Glu Gln 755 760 765 Pro Thr Ser Glu Ala Val Glu Ala Pro Leu Val Gly Thr Pro Val Cys 770 775 780 Ile Asn Gly Leu Met Leu Leu Glu Ile Lys Asp Thr Glu Lys Tyr Cys 785 790 795 800 Ala Leu Ala Pro Asn Met Met Val Thr Asn Asn Thr Phe Thr Leu Lys 805 810 815 Gly Gly Ala Pro Thr Lys Val Thr Phe Gly Asp Asp Thr Val Ile Glu 820 825 830 Val Gln Gly Tyr Lys Ser Val Asn Ile Thr Phe Glu Leu Asp Glu Arg 835 840 845 Ile Asp Lys Val Leu Asn Glu Lys Cys Ser Ala Tyr Thr Val Glu Leu 850 855 860 Gly Thr Glu Val Asn Glu Phe Ala Cys Val Val Ala Asp Ala Val Ile 865 870 875 880 Lys Thr Leu Gln Pro Val Ser Glu Leu Leu Thr Pro Leu Gly Ile Asp 885 890 895 Leu Asp Glu Trp Ser Met Ala Thr Tyr Tyr Leu Phe Asp Glu Ser Gly 900 905 910 Glu Phe Lys Leu Ala Ser His Met Tyr Cys Ser Phe Tyr Pro Pro Asp 915 920 925 Glu Asp Glu Glu Glu Gly Asp Cys Glu Glu Glu Glu Phe Glu Pro Ser 930 935 940 Thr Gln Tyr Glu Tyr Gly Thr Glu Asp Asp Tyr Gln Gly Lys Pro Leu 945 950 955 960 Glu Phe Gly Ala Thr Ser Ala Ala Leu Gln Pro Glu Glu Glu Gln Glu 965 970 975 Glu Asp Trp Leu Asp Asp Asp Ser Gln Gln Thr Val Gly Gln Gln Asp 980 985 990 Gly Ser Glu Asp Asn Gln Thr Thr Thr Ile Gln Thr Ile Val Glu Val 995 1000 1005 Gln Pro Gln Leu Glu Met Glu Leu Thr Pro Val Val Gln Thr Ile Glu 1010 1015 1020 Val Asn Ser Phe Ser Gly Tyr Leu Lys Leu Thr Asp Asn Val Tyr Ile 1025 1030 1035 1040 Lys Asn Ala Asp Ile Val Glu Glu Ala Lys Lys Val Lys Pro Thr Val 1045 1050 1055 Val Val Asn Ala Ala Asn Val Tyr Leu Lys His Gly Gly Gly Val Ala 1060 1065 1070 Gly Ala Leu Asn Lys Ala Thr Asn Asn Ala Met Gln Val Glu Ser Asp 1075 1080 1085 Asp Tyr Ile Ala Thr Asn Gly Pro Leu Lys Val Gly Gly Ser Cys Val 1090 1095 1100 Leu Ser Gly His Asn Leu Ala Lys His Cys Leu His Val Val Gly Pro 1105 1110 1115 1120 Asn Val Asn Lys Gly Glu Asp Ile Gln Leu Leu Lys Ser Ala Tyr Glu 1125 1130 1135 Asn Phe Asn Gln His Glu Val Leu Leu Ala Pro Leu Leu Ser Ala Gly 1140 1145 1150 Ile Phe Gly Ala Asp Pro Ile His Ser Leu Arg Val Cys Val Asp Thr 1155 1160 1165 Val Arg Thr Asn Val Tyr Leu Ala Val Phe Asp Lys Asn Leu Tyr Asp 1170 1175 1180 Lys Leu Val Ser Ser Phe Leu Glu Met Lys Ser Glu Lys Gln Val Glu 1185 1190 1195 1200 Gln Lys Ile Ala Glu Ile Pro Lys Glu Glu Val Lys Pro Phe Ile Thr 1205 1210 1215 Glu Ser Lys Pro Ser Val Glu Gln Arg Lys Gln Asp Asp Lys Lys Ile 1220 1225 1230 Lys Ala Cys Val Glu Glu Val Thr Thr Thr Leu Glu Glu Thr Lys Phe 1235 1240 1245 Leu Thr Glu Asn Leu Leu Leu Tyr Ile Asp Ile Asn Gly Asn Leu His 1250 1255 1260 Pro Asp Ser Ala Thr Leu Val Ser Asp Ile Asp Ile Thr Phe Leu Lys 1265 1270 1275 1280 Lys Asp Ala Pro Tyr Ile Val Gly Asp Val Val Gln Glu Gly Val Leu 1285 1290 1295 Thr Ala Val Val Ile Pro Thr Lys Lys Ala Gly Gly Thr Thr Glu Met 1300 1305 1310 Leu Ala Lys Ala Leu Arg Lys Val Pro Thr Asp Asn Tyr Ile Thr Thr 1315 1320 1325 Tyr Pro Gly Gln Gly Leu Asn Gly Tyr Thr Val Glu Glu Ala Lys Thr 1330 1335 1340 Val Leu Lys Lys Cys Lys Ser Ala Phe Tyr Ile Leu Pro Ser Ile Ile 1345 1350 1355 1360 Ser Asn Glu Lys Gln Glu Ile Leu Gly Thr Val Ser Trp Asn Leu Arg 1365 1370 1375 Glu Met Leu Ala His Ala Glu Glu Thr Arg Lys Leu Met Pro Val Cys 1380 1385 1390 Val Glu Thr Lys Ala Ile Val Ser Thr Ile Gln Arg Lys Tyr Lys Gly 1395 1400 1405 Ile Lys Ile Gln Glu Gly Val Val Asp Tyr Gly Ala Arg Phe Tyr Phe 1410 1415 1420 Tyr Thr Ser Lys Thr Thr Val Ala Ser Leu Ile Asn Thr Leu Asn Asp 1425 1430 1435 1440 Leu Asn Glu Thr Leu Val Thr Met Pro Leu Gly Tyr Val Thr His Gly 1445 1450 1455 Leu Asn Leu Glu Glu Ala Ala Arg Tyr Met Arg Ser Leu Lys Val Pro 1460 1465 1470 Ala Thr Val Ser Val Ser Ser Pro Asp Ala Val Thr Ala Tyr Asn Gly 1475 1480 1485 Tyr Leu Thr Ser Ser Ser Lys Thr Pro Glu Glu His Phe Ile Glu Thr 1490 1495 1500 Ile Ser Leu Ala Gly Ser Tyr Lys Asp Trp Ser Tyr Ser Gly Gln Ser 1505 1510 1515 1520 Thr Gln Leu Gly Ile Glu Phe Leu Lys Arg Gly Asp Lys Ser Val Tyr 1525 1530 1535 Tyr Thr Ser Asn Pro Thr Thr Phe His Leu Asp Gly Glu Val Ile Thr 1540 1545 1550 Phe Asp Asn Leu Lys Thr Leu Leu Ser Leu Arg Glu Val Arg Thr Ile 1555 1560 1565 Lys Val Phe Thr Thr Val Asp Asn Ile Asn Leu His Thr Gln Val Val 1570 1575 1580 Asp Met Ser Met Thr Tyr Gly Gln Gln Phe Gly Pro Thr Tyr Leu Asp 1585 1590 1595 1600 Gly Ala Asp Val Thr Lys Ile Lys Pro His Asn Ser His Glu Gly Lys 1605 1610 1615 Thr Phe Tyr Val Leu Pro Asn Asp Asp Thr Leu Arg Val Glu Ala Phe 1620 1625 1630 Glu Tyr Tyr His Thr Thr Asp Pro Ser Phe Leu Gly Arg Tyr Met Ser 1635 1640 1645 Ala Leu Asn His Thr Lys Lys Trp Lys Tyr Pro Gln Val Asn Gly Leu 1650 1655 1660 Thr Ser Ile Lys Trp Ala Asp Asn Asn Cys Tyr Leu Ala Thr Ala Leu 1665 1670 1675 1680 Leu Thr Leu Gln Gln Ile Glu Leu Lys Phe Asn Pro Pro Ala Leu Gln 1685 1690 1695 Asp Ala Tyr Tyr Arg Ala Arg Ala Gly Glu Ala Ala Asn Phe Cys Ala 1700 1705 1710 Leu Ile Leu Ala Tyr Cys Asn Lys Thr Val Gly Glu Leu Gly Asp Val 1715 1720 1725 Arg Glu Thr Met Ser Tyr Leu Phe Gln His Ala Asn Leu Asp Ser Cys 1730 1735 1740 Lys Arg Val Leu Asn Val Val Cys Lys Thr Cys Gly Gln Gln Gln Thr 1745 1750 1755 1760 Thr Leu Lys Gly Val Glu Ala Val Met Tyr Met Gly Thr Leu Ser Tyr 1765 1770 1775 Glu Gln Phe Lys Lys Gly Val Gln Ile Pro Cys Thr Cys Gly Lys Gln 1780 1785 1790 Ala Thr Lys Tyr Leu Val Gln Gln Glu Ser Pro Phe Val Met Met Ser 1795 1800 1805 Ala Pro Pro Ala Gln Tyr Glu Leu Lys His Gly Thr Phe Thr Cys Ala 1810 1815 1820 Ser Glu Tyr Thr Gly Asn Tyr Gln Cys Gly His Tyr Lys His Ile Thr 1825 1830 1835 1840 Ser Lys Glu Thr Leu Tyr Cys Ile Asp Gly Ala Leu Leu Thr Lys Ser 1845 1850 1855 Ser Glu Tyr Lys Gly Pro Ile Thr Asp Val Phe Tyr Lys Glu Asn Ser 1860 1865 1870 Tyr Thr Thr Thr Ile Lys Pro Val Thr Tyr Lys Leu Asp Gly Val Val 1875 1880 1885 Cys Thr Glu Ile Asp Pro Lys Leu Asp Asn Tyr Tyr Lys Lys Asp Asn 1890 1895 1900 Ser Tyr Phe Thr Glu Gln Pro Ile Asp Leu Val Pro Asn Gln Pro Tyr 1905 1910 1915 1920 Pro Asn Ala Ser Phe Asp Asn Phe Lys Phe Val Cys Asp Asn Ile Lys 1925 1930 1935 Phe Ala Asp Asp Leu Asn Gln Leu Thr Gly Tyr Lys Lys Pro Ala Ser 1940 1945 1950 Arg Glu Leu Lys Val Thr Phe Phe Pro Asp Leu Asn Gly Asp Val Val 1955 1960 1965 Ala Ile Asp Tyr Lys His Tyr Thr Pro Ser Phe Lys Lys Gly Ala Lys 1970 1975 1980 Leu Leu His Lys Pro Ile Val Trp His Val Asn Asn Ala Thr Asn Lys 1985 1990 1995 2000 Ala Thr Tyr Lys Pro Asn Thr Trp Cys Ile Arg Cys Leu Trp Ser Thr 2005 2010 2015 Lys Pro Val Glu Thr Ser Asn Ser Phe Asp Val Leu Lys Ser Glu Asp 2020 2025 2030 Ala Gln Gly Met Asp Asn Leu Ala Cys Glu Asp Leu Lys Pro Val Ser 2035 2040 2045 Glu Glu Val Val Glu Asn Pro Thr Ile Gln Lys Asp Val Leu Glu Cys 2050 2055 2060 Asn Val Lys Thr Thr Glu Val Val Gly Asp Ile Ile Leu Lys Pro Ala 2065 2070 2075 2080 Asn Asn Ser Leu Lys Ile Thr Glu Glu Val Gly His Thr Asp Leu Met 2085 2090 2095 Ala Ala Tyr Val Asp Asn Ser Ser Leu Thr Ile Lys Lys Pro Asn Glu 2100 2105 2110 Leu Ser Arg Val Leu Gly Leu Lys Thr Leu Ala Thr His Gly Leu Ala 2115 2120 2125 Ala Val Asn Ser Val Pro Trp Asp Thr Ile Ala Asn Tyr Ala Lys Pro 2130 2135 2140 Phe Leu Asn Lys Val Val Ser Thr Thr Thr Asn Ile Val Thr Arg Cys 2145 2150 2155 2160 Leu Asn Arg Val Cys Thr Asn Tyr Met Pro Tyr Phe Phe Thr Leu Leu 2165 2170 2175 Leu Gln Leu Cys Thr Phe Thr Arg Ser Thr Asn Ser Arg Ile Lys Ala 2180 2185 2190 Ser Met Pro Thr Thr Ile Ala Lys Asn Thr Val Lys Ser Val Gly Lys 2195 2200 2205 Phe Cys Leu Glu Ala Ser Phe Asn Tyr Leu Lys Ser Pro Asn Phe Ser 2210 2215 2220 Lys Leu Ile Asn Ile Ile Ile Trp Phe Leu Leu Leu Ser Val Cys Leu 2225 2230 2235 2240 Gly Ser Leu Ile Tyr Ser Thr Ala Ala Leu Gly Val Leu Met Ser Asn 2245 2250 2255 Leu Gly Met Pro Ser Tyr Cys Thr Gly Tyr Arg Glu Gly Tyr Leu Asn 2260 2265 2270 Ser Thr Asn Val Thr Ile Ala Thr Tyr Cys Thr Gly Ser Ile Pro Cys 2275 2280 2285 Ser Val Cys Leu Ser Gly Leu Asp Ser Leu Asp Thr Tyr Pro Ser Leu 2290 2295 2300 Glu Thr Ile Gln Ile Thr Ile Ser Ser Phe Lys Trp Asp Leu Thr Ala 2305 2310 2315 2320 Phe Gly Leu Val Ala Glu Trp Phe Leu Ala Tyr Ile Leu Phe Thr Arg 2325 2330 2335 Phe Phe Tyr Val Leu Gly Leu Ala Ala Ile Met Gln Leu Phe Phe Ser 2340 2345 2350 Tyr Phe Ala Val His Phe Ile Ser Asn Ser Trp Leu Met Trp Leu Ile 2355 2360 2365 Ile Asn Leu Val Gln Met Ala Pro Ile Ser Ala Met Val Arg Met Tyr 2370 2375 2380 Ile Phe Phe Ala Ser Phe Tyr Tyr Val Trp Lys Ser Tyr Val His Val 2385 2390 2395 2400 Val Asp Gly Cys Asn Ser Ser Thr Cys Met Met Cys Tyr Lys Arg Asn 2405 2410 2415 Arg Ala Thr Arg Val Glu Cys Thr Thr Ile Val Asn Gly Val Arg Arg 2420 2425 2430 Ser Phe Tyr Val Tyr Ala Asn Gly Gly Lys Gly Phe Cys Lys Leu His 2435 2440 2445 Asn Trp Asn Cys Val Asn Cys Asp Thr Phe Cys Ala Gly Ser Thr Phe 2450 2455 2460 Ile Ser Asp Glu Val Ala Arg Asp Leu Ser Leu Gln Phe Lys Arg Pro 2465 2470 2475 2480 Ile Asn Pro Thr Asp Gln Ser Ser Tyr Ile Val Asp Ser Val Thr Val 2485 2490 2495 Lys Asn Gly Ser Ile His Leu Tyr Phe Asp Lys Ala Gly Gln Lys Thr 2500 2505 2510 Tyr Glu Arg His Ser Leu Ser His Phe Val Asn Leu Asp Asn Leu Arg 2515 2520 2525 Ala Asn Asn Thr Lys Gly Ser Leu Pro Ile Asn Val Ile Val Phe Asp 2530 2535 2540 Gly Lys Ser Lys Cys Glu Glu Ser Ser Ala Lys Ser Ala Ser Val Tyr 2545 2550 2555 2560 Tyr Ser Gln Leu Met Cys Gln Pro Ile Leu Leu Leu Asp Gln Ala Leu 2565 2570 2575 Val Ser Asp Val Gly Asp Ser Ala Glu Val Ala Val Lys Met Phe Asp 2580 2585 2590 Ala Tyr Val Asn Thr Phe Ser Ser Thr Phe Asn Val Pro Met Glu Lys 2595 2600 2605 Leu Lys Thr Leu Val Ala Thr Ala Glu Ala Glu Leu Ala Lys Asn Val 2610 2615 2620 Ser Leu Asp Asn Val Leu Ser Thr Phe Ile Ser Ala Ala Arg Gln Gly 2625 2630 2635 2640 Phe Val Asp Ser Asp Val Glu Thr Lys Asp Val Val Glu Cys Leu Lys 2645 2650 2655 Leu Ser His Gln Ser Asp Ile Glu Val Thr Gly Asp Ser Cys Asn Asn 2660 2665 2670 Tyr Met Leu Thr Tyr Asn Lys Val Glu Asn Met Thr Pro Arg Asp Leu 2675 2680 2685 Gly Ala Cys Ile Asp Cys Ser Ala Arg His Ile Asn Ala Gln Val Ala 2690 2695 2700 Lys Ser His Asn Ile Ala Leu Ile Trp Asn Val Lys Asp Phe Met Ser 2705 2710 2715 2720 Leu Ser Glu Gln Leu Arg Lys Gln Ile Arg Ser Ala Ala Lys Lys Asn 2725 2730 2735 Asn Leu Pro Phe Lys Leu Thr Cys Ala Thr Thr Arg Gln Val Val Asn 2740 2745 2750 Val Val Thr Thr Lys Ile Ala Leu Lys Gly Gly Lys Ile Val Asn Asn 2755 2760 2765 Trp Leu Lys Gln Leu Ile Lys Val Thr Leu Val Phe Leu Phe Val Ala 2770 2775 2780 Ala Ile Phe Tyr Leu Ile Thr Pro Val His Val Met Ser Lys His Thr 2785 2790 2795 2800 Asp Phe Ser Ser Glu Ile Ile Gly Tyr Lys Ala Ile Asp Gly Gly Val 2805 2810 2815 Thr Arg Asp Ile Ala Ser Thr Asp Thr Cys Phe Ala Asn Lys His Ala 2820 2825 2830 Asp Phe Asp Thr Trp Phe Ser Gln Arg Gly Gly Ser Tyr Thr Asn Asp 2835 2840 2845 Lys Ala Cys Pro Leu Ile Ala Ala Val Ile Thr Arg Glu Val Gly Phe 2850 2855 2860 Val Val Pro Gly Leu Pro Gly Thr Ile Leu Arg Thr Thr Asn Gly Asp 2865 2870 2875 2880 Phe Leu His Phe Leu Pro Arg Val Phe Ser Ala Val Gly Asn Ile Cys 2885 2890 2895 Tyr Thr Pro Ser Lys Leu Ile Glu Tyr Thr Asp Phe Ala Thr Ser Ala 2900 2905 2910 Cys Val Leu Ala Ala Glu Cys Thr Ile Phe Lys Asp Ala Ser Gly Lys 2915 2920 2925 Pro Val Pro Tyr Cys Tyr Asp Thr Asn Val Leu Glu Gly Ser Val Ala 2930 2935 2940 Tyr Glu Ser Leu Arg Pro Asp Thr Arg Tyr Val Leu Met Asp Gly Ser 2945 2950 2955 2960 Ile Ile Gln Phe Pro Asn Thr Tyr Leu Glu Gly Ser Val Arg Val Val 2965 2970 2975 Thr Thr Phe Asp Ser Glu Tyr Cys Arg His Gly Thr Cys Glu Arg Ser 2980 2985 2990 Glu Ala Gly Val Cys Val Ser Thr Ser Gly Arg Trp Val Leu Asn Asn 2995 3000 3005 Asp Tyr Tyr Arg Ser Leu Pro Gly Val Phe Cys Gly Val Asp Ala Val 3010 3015 3020 Asn Leu Leu Thr Asn Met Phe Thr Pro Leu Ile Gln Pro Ile Gly Ala 3025 3030 3035 3040 Leu Asp Ile Ser Ala Ser Ile Val Ala Gly Gly Ile Val Ala Ile Val 3045 3050 3055 Val Thr Cys Leu Ala Tyr Tyr Phe Met Arg Phe Arg Arg Ala Phe Gly 3060 3065 3070 Glu Tyr Ser His Val Val Ala Phe Asn Thr Leu Leu Phe Leu Met Ser 3075 3080 3085 Phe Thr Val Leu Cys Leu Thr Pro Val Tyr Ser Phe Leu Pro Gly Val 3090 3095 3100 Tyr Ser Val Ile Tyr Leu Tyr Leu Thr Phe Tyr Leu Thr Asn Asp Val 3105 3110 3115 3120 Ser Phe Leu Ala His Ile Gln Trp Met Val Met Phe Thr Pro Leu Val 3125 3130 3135 Pro Phe Trp Ile Thr Ile Ala Tyr Ile Ile Cys Ile Ser Thr Lys His 3140 3145 3150 Phe Tyr Trp Phe Phe Ser Asn Tyr Leu Lys Arg Arg Val Val Phe Asn 3155 3160 3165 Gly Val Ser Phe Ser Thr Phe Glu Glu Ala Ala Leu Cys Thr Phe Leu 3170 3175 3180 Leu Asn Lys Glu Met Tyr Leu Lys Leu Arg Ser Asp Val Leu Leu Pro 3185 3190 3195 3200 Leu Thr Gln Tyr Asn Arg Tyr Leu Ala Leu Tyr Asn Lys Tyr Lys Tyr 3205 3210 3215 Phe Ser Gly Ala Met Asp Thr Thr Ser Tyr Arg Glu Ala Ala Cys Cys 3220 3225 3230 His Leu Ala Lys Ala Leu Asn Asp Phe Ser Asn Ser Gly Ser Asp Val 3235 3240 3245 Leu Tyr Gln Pro Pro Gln Thr Ser Ile Thr Ser Ala Val Leu Gln Ser 3250 3255 3260 Gly Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys Met 3265 3270 3275 3280 Val Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu Asp 3285 3290 3295 Asp Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp Met 3300 3305 3310 Leu Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His Asn 3315 3320 3325 Phe Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His Ser 3330 3335 3340 Met Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro Lys 3345 3350 3355 3360 Thr Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe Ser 3365 3370 3375 Val Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys Ala 3380 3385 3390 Met Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser Cys 3395 3400 3405 Gly Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys Tyr 3410 3415 3420 Met His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp Leu 3425 3430 3435 3440 Glu Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln Ala 3445 3450 3455 Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu Tyr 3460 3465 3470 Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr Thr 3475 3480 3485 Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu Pro 3490 3495 3500 Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln Thr 3505 3510 3515 3520 Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu Gln 3525 3530 3535 Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu Asp 3540 3545 3550 Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr Phe 3555 3560 3565 Gln Ser Ala Val Lys Arg Thr Ile Lys Gly Thr His His Trp Leu Leu 3570 3575 3580 Leu Thr Ile Leu Thr Ser Leu Leu Val Leu Val Gln Ser Thr Gln Trp 3585 3590 3595 3600 Ser Leu Phe Phe Phe Xaa Tyr Glu Asn Ala Phe Leu Pro Phe Ala Met 3605 3610 3615 Gly Ile Ile Ala Met Ser Ala Phe Ala Met Met Phe Val Lys His Lys 3620 3625 3630 His Ala Phe Leu Cys Leu Phe Leu Leu Pro Ser Leu Ala Thr Val Ala 3635 3640 3645 Tyr Phe Asn Met Val Tyr Met Pro Ala Ser Trp Val Met Arg Ile Met 3650 3655 3660 Thr Trp Leu Asp Met Val Asp Thr Ser Leu Ser Gly Phe Lys Leu Lys 3665 3670 3675 3680 Asp Cys Val Met Tyr Ala Ser Ala Val Val Leu Leu Ile Leu Met Thr 3685 3690 3695 Ala Arg Thr Val Tyr Asp Asp Gly Ala Arg Arg Val Trp Thr Leu Met 3700 3705 3710 Asn Val Leu Thr Leu Val Tyr Lys Val Tyr Tyr Gly Asn Ala Leu Asp 3715 3720 3725 Gln Ala Ile Ser Met Trp Ala Leu Ile Ile Ser Val Thr Ser Asn Tyr 3730 3735 3740 Ser Gly Val Val Thr Thr Val Met Phe Leu Ala Arg Gly Ile Val Phe 3745 3750 3755 3760 Met Cys Val Glu Tyr Cys Pro Ile Phe Phe Ile Thr Gly Asn Thr Leu 3765 3770 3775 Gln Cys Ile Met Leu Val Tyr Cys Phe Leu Gly Tyr Phe Cys Thr Cys 3780 3785 3790 Tyr Phe Gly Leu Phe Cys Leu Leu Asn Arg Tyr Phe Arg Leu Thr Leu 3795 3800 3805 Gly Val Tyr Asp Tyr Leu Val Ser Thr Gln Glu Phe Arg Tyr Met Asn 3810 3815 3820 Ser Gln Gly Leu Leu Pro Pro Lys Asn Ser Ile Asp Ala Phe Lys Leu 3825 3830 3835 3840 Asn Ile Lys Leu Leu Gly Val Gly Gly Lys Pro Cys Ile Lys Val Ala 3845 3850 3855 Thr Val Gln Ser Lys Met Ser Asp Val Lys Cys Thr Ser Val Val Leu 3860 3865 3870 Leu Ser Val Leu Gln Gln Leu Arg Val Glu Ser Ser Ser Lys Leu Trp 3875 3880 3885 Ala Gln Cys Val Gln Leu His Asn Asp Ile Leu Leu Ala Lys Asp Thr 3890 3895 3900 Thr Glu Ala Phe Glu Lys Met Val Ser Leu Leu Ser Val Leu Leu Ser 3905 3910 3915 3920 Met Gln Gly Ala Val Asp Ile Asn Lys Leu Cys Glu Glu Met Leu Asp 3925 3930 3935 Asn Arg Ala Thr Leu Gln Ala Ile Ala Ser Glu Phe Ser Ser Leu Pro 3940 3945 3950 Ser Tyr Ala Ala Phe Ala Thr Ala Gln Glu Ala Tyr Glu Gln Ala Val 3955 3960 3965 Ala Asn Gly Asp Ser Glu Val Val Leu Lys Lys Leu Lys Lys Ser Leu 3970 3975 3980 Asn Val Ala Lys Ser Glu Phe Asp Arg Asp Ala Ala Met Gln Arg Lys 3985 3990 3995 4000 Leu Glu Lys Met Ala Asp Gln Ala Met Thr Gln Met Tyr Lys Gln Ala 4005 4010 4015 Arg Ser Glu Asp Lys Arg Ala Lys Val Thr Ser Ala Met Gln Thr Met 4020 4025 4030 Leu Phe Thr Met Leu Arg Lys Leu Asp Asn Asp Ala Leu Asn Asn Ile 4035 4040 4045 Ile Asn Asn Ala Arg Asp Gly Cys Val Pro Leu Asn Ile Ile Pro Leu 4050 4055 4060 Thr Thr Ala Ala Lys Leu Met Val Val Ile Pro Asp Tyr Asn Thr Tyr 4065 4070 4075 4080 Lys Asn Thr Cys Asp Gly Thr Thr Phe Thr Tyr Ala Ser Ala Leu Trp 4085 4090 4095 Glu Ile Gln Gln Val Val Asp Ala Asp Ser Lys Ile Val Gln Leu Ser 4100 4105 4110 Glu Ile Ser Met Asp Asn Ser Pro Asn Leu Ala Trp Pro Leu Ile Val 4115 4120 4125 Thr Ala Leu Arg Ala Asn Ser Ala Val Lys Leu Gln Asn Asn Glu Leu 4130 4135 4140 Ser Pro Val Ala Leu Arg Gln Met Ser Cys Ala Ala Gly Thr Thr Gln 4145 4150 4155 4160 Thr Ala Cys Thr Asp Asp Asn Ala Leu Ala Tyr Tyr Asn Thr Thr Lys 4165 4170 4175 Gly Gly Arg Phe Val Leu Ala Leu Leu Ser Asp Leu Gln Asp Leu Lys 4180 4185 4190 Trp Ala Arg Phe Pro Lys Ser Asp Gly Thr Gly Thr Ile Tyr Thr Glu 4195 4200 4205 Leu Glu Pro Pro Cys Arg Phe Val Thr Asp Thr Pro Lys Gly Pro Lys 4210 4215 4220 Val Lys Tyr Leu Tyr Phe Ile Lys Gly Leu Asn Asn Leu Asn Arg Gly 4225 4230 4235 4240 Met Val Leu Gly Ser Leu Ala Ala Thr Val Arg Leu Gln Ala Gly Asn 4245 4250 4255 Ala Thr Glu Val Pro Ala Asn Ser Thr Val Leu Ser Phe Cys Ala Phe 4260 4265 4270 Ala Val Asp Ala Ala Lys Ala Tyr Lys Asp Tyr Leu Ala Ser Gly Gly 4275 4280 4285 Gln Pro Ile Thr Asn Cys Val Lys Met Leu Cys Thr His Thr Gly Thr 4290 4295 4300 Gly Gln Ala Ile Thr Val Thr Pro Glu Ala Asn Met Asp Gln Glu Ser 4305 4310 4315 4320 Phe Gly Gly Ala Ser Cys Cys Leu Tyr Cys Arg Cys His Ile Asp His 4325 4330 4335 Pro Asn Pro Lys Gly Phe Cys Asp Leu Lys Gly Lys Tyr Val Gln Ile 4340 4345 4350 Pro Thr Thr Cys Ala Asn Asp Pro Val Gly Phe Thr Leu Lys Asn Thr 4355 4360 4365 Val Cys Thr Val Cys Gly Met Trp Lys Gly Tyr Gly Cys Ser Cys Asp 4370 4375 4380 Gln Leu Arg Glu Pro Met Leu Gln Ser Ala Asp Ala Gln Ser Phe Leu 4385 4390 4395 4400 Asn Arg Val Cys Gly Val Ser Ala Ala Arg Leu Thr Pro Cys Gly Thr 4405 4410 4415 Gly Thr Ser Thr Asp Val Val Tyr Arg Ala Phe Asp Ile Tyr Asn Asp 4420 4425 4430 Lys Val Ala Gly Phe Ala Lys Phe Leu Lys Thr Asn Cys Cys Arg Phe 4435 4440 4445 Gln Glu Lys Asp Glu Asp Asp Asn Leu Ile Asp Ser Tyr Phe Val Val 4450 4455 4460 Lys Arg His Thr Phe Ser Asn Tyr Gln His Glu Glu Thr Ile Tyr Asn 4465 4470 4475 4480 Leu Leu Lys Asp Cys Pro Ala Val Ala Lys His Asp Phe Phe Lys Phe 4485 4490 4495 Arg Ile Asp Gly Asp Met Val Pro His Ile Ser Arg Gln Arg Leu Thr 4500 4505 4510 Lys Tyr Thr Met Ala Asp Leu Val Tyr Ala Leu Arg His Phe Asp Glu 4515 4520 4525 Gly Asn Cys Asp Thr Leu Lys Glu Ile Leu Val Thr Tyr Asn Cys Cys 4530 4535 4540 Asp Asp Asp Tyr Phe Asn Lys Lys Asp Trp Tyr Asp Phe Val Glu Asn 4545 4550 4555 4560 Pro Asp Ile Leu Arg Val Tyr Ala Asn Leu Gly Glu Arg Val Arg Gln 4565 4570 4575 Ala Leu Leu Lys Thr Val Gln Phe Cys Asp Ala Met Arg Asn Ala Gly 4580 4585 4590 Ile Val Gly Val Leu Thr Leu Asp Asn Gln Asp Leu Asn Gly Asn Trp 4595 4600 4605 Tyr Asp Phe Gly Asp Phe Ile Gln Thr Thr Pro Gly Ser Gly Val Pro 4610 4615 4620 Val Val Asp Ser Tyr Tyr Ser Leu Leu Met Pro Ile Leu Thr Leu Thr 4625 4630 4635 4640 Arg Ala Leu Thr Ala Glu Ser His Val Asp Thr Asp Leu Thr Lys Pro 4645 4650 4655 Tyr Ile Lys Trp Asp Leu Leu Lys Tyr Asp Phe Thr Glu Glu Arg Leu 4660 4665 4670 Lys Leu Phe Asp Arg Tyr Phe Lys Tyr Trp Asp Gln Thr Tyr His Pro 4675 4680 4685 Asn Cys Val Asn Cys Leu Asp Asp Arg Cys Ile Leu His Cys Ala Asn 4690 4695 4700 Phe Asn Val Leu Phe Ser Thr Val Phe Pro Pro Thr Ser Phe Gly Pro 4705 4710 4715 4720 Leu Val Arg Lys Ile Phe Val Asp Gly Val Pro Phe Val Val Ser Thr 4725 4730 4735 Gly Tyr His Phe Arg Glu Leu Gly Val Val His Asn Gln Asp Val Asn 4740 4745 4750 Leu His Ser Ser Arg Leu Ser Phe Lys Glu Leu Leu Val Tyr Ala Ala 4755 4760 4765 Asp Pro Ala Met His Ala Ala Ser Gly Asn Leu Leu Leu Asp Lys Arg 4770 4775 4780 Thr Thr Cys Phe Ser Val Ala Ala Leu Thr Asn Asn Val Ala Phe Gln 4785 4790 4795 4800 Thr Val Lys Pro Gly Asn Phe Asn Lys Asp Phe Tyr Asp Phe Ala Val 4805 4810 4815 Ser Lys Gly Phe Phe Lys Glu Gly Ser Ser Val Glu Leu Lys His Phe 4820 4825 4830 Phe Phe Ala Gln Asp Gly Asn Ala Ala Ile Ser Asp Tyr Asp Tyr Tyr 4835 4840 4845 Arg Tyr Asn Leu Pro Thr Met Cys Asp Ile Arg Gln Leu Leu Phe Val 4850 4855 4860 Val Glu Val Val Asp Lys Tyr Phe Asp Cys Tyr Asp Gly Gly Cys Ile 4865 4870 4875 4880 Asn Ala Asn Gln Val Ile Val Asn Asn Leu Asp Lys Ser Ala Gly Phe 4885 4890 4895 Pro Phe Asn Lys Trp Gly Lys Ala Arg Leu Tyr Tyr Asp Ser Met Ser 4900 4905 4910 Tyr Glu Asp Gln Asp Ala Leu Phe Ala Tyr Thr Lys Arg Asn Val Ile 4915 4920 4925 Pro Thr Ile Thr Gln Met Asn Leu Lys Tyr Ala Ile Ser Ala Lys Asn 4930 4935 4940 Arg Ala Arg Thr Val Ala Gly Val Ser Ile Cys Ser Thr Met Thr Asn 4945 4950 4955 4960 Arg Gln Phe His Gln Lys Leu Leu Lys Ser Ile Ala Ala Thr Arg Gly 4965 4970 4975 Ala Thr Val Val Ile Gly Thr Ser Lys Phe Tyr Gly Gly Trp His Asn 4980 4985 4990 Met Leu Lys Thr Val Tyr Ser Asp Val Glu Asn Pro His Leu Met Gly 4995 5000 5005 Trp Asp Tyr Pro Lys Cys Asp Arg Ala Met Pro Asn Met Leu Arg Ile 5010 5015 5020 Met Ala Ser Leu Val Leu Ala Arg Lys His Thr Thr Cys Cys Ser Leu 5025 5030 5035 5040 Ser His Arg Phe Tyr Arg Leu Ala Asn Glu Cys Ala Gln Val Leu Ser 5045 5050 5055 Glu Met Val Met Cys Gly Gly Ser Leu Tyr Val Lys Pro Gly Gly Thr 5060 5065 5070 Ser Ser Gly Asp Ala Thr Thr Ala Tyr Ala Asn Ser Val Phe Asn Ile 5075 5080 5085 Cys Gln Ala Val Thr Ala Asn Val Asn Ala Leu Leu Ser Thr Asp Gly 5090 5095 5100 Asn Lys Ile Ala Asp Lys Tyr Val Arg Asn Leu Gln His Arg Leu Tyr 5105 5110 5115 5120 Glu Cys Leu Tyr Arg Asn Arg Asp Val Asp Thr Asp Phe Val Asn Glu 5125 5130 5135 Phe Tyr Ala Tyr Leu Arg Lys His Phe Ser Met Met Ile Leu Ser Asp 5140 5145 5150 Asp Ala Val Val Cys Phe Asn Ser Thr Tyr Ala Ser Gln Gly Leu Val 5155 5160 5165 Ala Ser Ile Lys Asn Phe Lys Ser Val Leu Tyr Tyr Gln Asn Asn Val 5170 5175 5180 Phe Met Ser Glu Ala Lys Cys Trp Thr Glu Thr Asp Leu Thr Lys Gly 5185 5190 5195 5200 Pro His Glu Phe Cys Ser Gln His Thr Met Leu Val Lys Gln Gly Asp 5205 5210 5215 Asp Tyr Val Tyr Leu Pro Tyr Pro Asp Pro Ser Arg Ile Leu Gly Ala 5220 5225 5230 Gly Cys Phe Val Asp Asp Ile Val Lys Thr Asp Gly Thr Leu Met Ile 5235 5240 5245 Glu Arg Phe Val Ser Leu Ala Ile Asp Ala Tyr Pro Leu Thr Lys His 5250 5255 5260 Pro Asn Gln Glu Tyr Ala Asp Val Phe His Leu Tyr Leu Gln Tyr Ile 5265 5270 5275 5280 Arg Lys Leu His Asp Glu Leu Thr Gly His Met Leu Asp Met Tyr Ser 5285 5290 5295 Val Met Leu Thr Asn Asp Asn Thr Ser Arg Tyr Trp Glu Pro Glu Phe 5300 5305 5310 Tyr Glu Ala Met Tyr Thr Pro His Thr Val Leu Gln Ala Val Gly Ala 5315 5320 5325 Cys Val Leu Cys Asn Ser Gln Thr Ser Leu Arg Cys Gly Ala Cys Ile 5330 5335 5340 Arg Arg Pro Phe Leu Cys Cys Lys Cys Cys Tyr Asp His Val Ile Ser 5345 5350 5355 5360 Thr Ser His Lys Leu Val Leu Ser Val Asn Pro Tyr Val Cys Asn Ala 5365 5370 5375 Pro Gly Cys Asp Val Thr Asp Val Thr Gln Leu Tyr Leu Gly Gly Met 5380 5385 5390 Ser Tyr Tyr Cys Lys Ser His Lys Pro Pro Ile Ser Phe Pro Leu Cys 5395 5400 5405 Ala Asn Gly Gln Val Phe Gly Leu Tyr Lys Asn Thr Cys Val Gly Ser 5410 5415 5420 Asp Asn Val Thr Asp Phe Asn Ala Ile Ala Thr Cys Asp Trp Thr Asn 5425 5430 5435 5440 Ala Gly Asp Tyr Ile Leu Ala Asn Thr Cys Thr Glu Arg Leu Lys Leu 5445 5450 5455 Phe Ala Ala Glu Thr Leu Lys Ala Thr Glu Glu Thr Phe Lys Leu Ser 5460 5465 5470 Tyr Gly Ile Ala Thr Val Arg Glu Val Leu Ser Asp Arg Glu Leu His 5475 5480 5485 Leu Ser Trp Glu Val Gly Lys Pro Arg Pro Pro Leu Asn Arg Asn Tyr 5490 5495 5500 Val Phe Thr Gly Tyr Arg Val Thr Lys Asn Ser Lys Val Gln Ile Gly 5505 5510 5515 5520 Glu Tyr Thr Phe Glu Lys Gly Asp Tyr Gly Asp Ala Val Val Tyr Arg 5525 5530 5535 Gly Thr Thr Thr Tyr Lys Leu Asn Val Gly Asp Tyr Phe Val Leu Thr 5540 5545 5550 Ser His Thr Val Met Pro Leu Ser Ala Pro Thr Leu Val Pro Gln Glu 5555 5560 5565 His Tyr Val Arg Ile Thr Gly Leu Tyr Pro Thr Leu Asn Ile Ser Asp 5570 5575 5580 Glu Phe Ser Ser Asn Val Ala Asn Tyr Gln Lys Val Gly Met Gln Lys 5585 5590 5595 5600 Tyr Ser Thr Leu Gln Gly Pro Pro Gly Thr Gly Lys Ser His Phe Ala 5605 5610 5615 Ile Gly Leu Ala Leu Tyr Tyr Pro Ser Ala Arg Ile Val Tyr Thr Ala 5620 5625 5630 Cys Ser His Ala Ala Val Asp Ala Leu Cys Glu Lys Ala Leu Lys Tyr 5635 5640 5645 Leu Pro Ile Asp Lys Cys Ser Arg Ile Ile Pro Ala Arg Ala Arg Val 5650 5655 5660 Glu Cys Phe Asp Lys Phe Lys Val Asn Ser Thr Leu Glu Gln Tyr Val 5665 5670 5675 5680 Phe Cys Thr Val Asn Ala Leu Pro Glu Thr Thr Ala Asp Ile Val Val 5685 5690 5695 Phe Asp Glu Ile Ser Met Ala Thr Asn Tyr Asp Leu Ser Val Val Asn 5700 5705 5710 Ala Arg Leu Arg Ala Lys His Tyr Val Tyr Ile Gly Asp Pro Ala Gln 5715 5720 5725 Leu Pro Ala Pro Arg Thr Leu Leu Thr Lys Gly Thr Leu Glu Pro Glu 5730 5735 5740 Tyr Phe Asn Ser Val Cys Arg Leu Met Lys Thr Ile Gly Pro Asp Met 5745 5750 5755 5760 Phe Leu Gly Thr Cys Arg Arg Cys Pro Ala Glu Ile Val Asp Thr Val 5765 5770 5775 Ser Ala Leu Val Tyr Asp Asn Lys Leu Lys Ala His Lys Asp Lys Ser 5780 5785 5790 Ala Gln Cys Phe Lys Met Phe Tyr Lys Gly Val Ile Thr His Asp Val 5795 5800 5805 Ser Ser Ala Ile Asn Arg Pro Gln Ile Gly Val Val Arg Glu Phe Leu 5810 5815 5820 Thr Arg Asn Pro Ala Trp Arg Lys Ala Val Phe Ile Ser Pro Tyr Asn 5825 5830 5835 5840 Ser Gln Asn Ala Val Ala Ser Lys Ile Leu Gly Leu Pro Thr Gln Thr 5845 5850 5855 Val Asp Ser Ser Gln Gly Ser Glu Tyr Asp Tyr Val Ile Phe Thr Gln 5860 5865 5870 Thr Thr Glu Thr Ala His Ser Cys Asn Val Asn Arg Phe Asn Val Ala 5875 5880 5885 Ile Thr Arg Ala Lys Val Gly Ile Leu Cys Ile Met Ser Asp Arg Asp 5890 5895 5900 Leu Tyr Asp Lys Leu Gln Phe Thr Ser Leu Glu Ile Pro Arg Arg Asn 5905 5910 5915 5920 Val Ala Thr Leu Gln Ala Glu Asn Val Thr Gly Leu Phe Lys Asp Cys 5925 5930 5935 Ser Lys Val Ile Thr Gly Leu His Pro Thr Gln Ala Pro Thr His Leu 5940 5945 5950 Ser Val Asp Thr Lys Phe Lys Thr Glu Gly Leu Cys Val Asp Ile Pro 5955 5960 5965 Gly Ile Pro Lys Asp Met Thr Tyr Arg Arg Leu Ile Ser Met Met Gly 5970 5975 5980 Phe Lys Met Asn Tyr Gln Val Asn Gly Tyr Pro Asn Met Phe Ile Thr 5985 5990 5995 6000 Arg Glu Glu Ala Ile Arg His Val Arg Ala Trp Ile Gly Phe Asp Val 6005 6010 6015 Glu Gly Cys His Ala Thr Arg Glu Ala Val Gly Thr Asn Leu Pro Leu 6020 6025 6030 Gln Leu Gly Phe Ser Thr Gly Val Asn Leu Val Ala Val Pro Thr Gly 6035 6040 6045 Tyr Val Asp Thr Pro Asn Asn Thr Asp Phe Ser Arg Val Ser Ala Lys 6050 6055 6060 Pro Pro Pro Gly Asp Gln Phe Lys His Leu Ile Pro Leu Met Tyr Lys 6065 6070 6075 6080 Gly Leu Pro Trp Asn Val Val Arg Ile Lys Ile Val Gln Met Leu Ser 6085 6090 6095 Asp Thr Leu Lys Asn Leu Ser Asp Arg Val Val Phe Val Leu Trp Ala 6100 6105 6110 His Gly Phe Glu Leu Thr Ser Met Lys Tyr Phe Val Lys Ile Gly Pro 6115 6120 6125 Glu Arg Thr Cys Cys Leu Cys Asp Arg Arg Ala Thr Cys Phe Ser Thr 6130 6135 6140 Ala Ser Asp Thr Tyr Ala Cys Trp His His Ser Ile Gly Phe Asp Tyr 6145 6150 6155 6160 Val Tyr Asn Pro Phe Met Ile Asp Val Gln Gln Trp Gly Phe Thr Gly 6165 6170 6175 Asn Leu Gln Ser Asn His Asp Leu Tyr Cys Gln Val His Gly Asn Ala 6180 6185 6190 His Val Ala Ser Cys Asp Ala Ile Met Thr Arg Cys Leu Ala Val His 6195 6200 6205 Glu Cys Phe Val Lys Arg Val Asp Trp Thr Ile Glu Tyr Pro Ile Ile 6210 6215 6220 Gly Asp Glu Leu Lys Ile Asn Ala Ala Cys Arg Lys Val Gln His Met 6225 6230 6235 6240 Val Val Lys Ala Ala Leu Leu Ala Asp Lys Phe Pro Val Leu His Asp 6245 6250 6255 Ile Gly Asn Pro Lys Ala Ile Lys Cys Val Pro Gln Ala Asp Val Glu 6260 6265 6270 Trp Lys Phe Tyr Asp Ala Gln Pro Cys Ser Asp Lys Ala Tyr Lys Ile 6275 6280 6285 Glu Glu Leu Phe Tyr Ser Tyr Ala Thr His Ser Asp Lys Phe Thr Asp 6290 6295 6300 Gly Val Cys Leu Phe Trp Asn Cys Asn Val Asp Arg Tyr Pro Ala Asn 6305 6310 6315 6320 Ser Ile Val Cys Arg Phe Asp Thr Arg Val Leu Ser Asn Leu Asn Leu 6325 6330 6335 Pro Gly Cys Asp Gly Gly Ser Leu Tyr Val Asn Lys His Ala Phe His 6340 6345 6350 Thr Pro Ala Phe Asp Lys Ser Ala Phe Val Asn Leu Lys Gln Leu Pro 6355 6360 6365 Phe Phe Tyr Tyr Ser Asp Ser Pro Cys Glu Ser His Gly Lys Gln Val 6370 6375 6380 Val Ser Asp Ile Asp Tyr Val Pro Leu Lys Ser Ala Thr Cys Ile Thr 6385 6390 6395 6400 Arg Cys Asn Leu Gly Gly Ala Val Cys Arg His His Ala Asn Glu Tyr 6405 6410 6415 Arg Leu Tyr Leu Asp Ala Tyr Asn Met Met Ile Ser Ala Gly Phe Ser 6420 6425 6430 Leu Trp Val Tyr Lys Gln Phe Asp Thr Tyr Asn Leu Trp Asn Thr Phe 6435 6440 6445 Thr Arg Leu Gln Ser Leu Glu Asn Val Ala Phe Asn Val Val Asn Lys 6450 6455 6460 Gly His Phe Asp Gly Gln Gln Gly Glu Val Pro Val Ser Ile Ile Asn 6465 6470 6475 6480 Asn Thr Val Tyr Thr Lys Val Asp Gly Val Asp Val Glu Leu Phe Glu 6485 6490 6495 Asn Lys Thr Thr Leu Pro Val Asn Val Ala Phe Glu Leu Trp Ala Lys 6500 6505 6510 Arg Asn Ile Lys Pro Val Pro Glu Val Lys Ile Leu Asn Asn Leu Gly 6515 6520 6525 Val Asp Ile Ala Ala Asn Thr Val Ile Trp Asp Tyr Lys Arg Asp Ala 6530 6535 6540 Pro Ala His Ile Ser Thr Ile Gly Val Cys Ser Met Thr Asp Ile Ala 6545 6550 6555 6560 Lys Lys Pro Thr Glu Thr Ile Cys Ala Pro Leu Thr Val Phe Phe Asp 6565 6570 6575 Gly Arg Val Asp Gly Gln Val Asp Leu Phe Arg Asn Ala Arg Asn Gly 6580 6585 6590 Val Leu Ile Thr Glu Gly Ser Val Lys Gly Leu Gln Pro Ser Val Gly 6595 6600 6605 Pro Lys Gln Ala Ser Leu Asn Gly Val Thr Leu Ile Gly Glu Ala Val 6610 6615 6620 Lys Thr Gln Phe Asn Tyr Tyr Lys Lys Val Asp Gly Val Val Gln Gln 6625 6630 6635 6640 Leu Pro Glu Thr Tyr Phe Thr Gln Ser Arg Asn Leu Gln Glu Phe Lys 6645 6650 6655 Pro Arg Ser Gln Met Glu Ile Asp Phe Leu Glu Leu Ala Met Asp Glu 6660 6665 6670 Phe Ile Glu Arg Tyr Lys Leu Glu Gly Tyr Ala Phe Glu His Ile Val 6675 6680 6685 Tyr Gly Asp Phe Ser His Ser Gln Leu Gly Gly Leu His Leu Leu Ile 6690 6695 6700 Gly Leu Ala Lys Arg Phe Lys Glu Ser Pro Phe Glu Leu Glu Asp Phe 6705 6710 6715 6720 Ile Pro Met Asp Ser Thr Val Lys Asn Tyr Phe Ile Thr Asp Ala Gln 6725 6730 6735 Thr Gly Ser Ser Lys Cys Val Cys Ser Val Ile Asp Leu Leu Leu Asp 6740 6745 6750 Asp Phe Val Glu Ile Ile Lys Ser Gln Asp Leu Ser Val Val Ser Lys 6755 6760 6765 Val Val Lys Val Thr Ile Asp Tyr Thr Glu Ile Ser Phe Met Leu Trp 6770 6775 6780 Cys Lys Asp Gly His Val Glu Thr Phe Tyr Pro Lys Leu Gln Ser Ser 6785 6790 6795 6800 Gln Ala Trp Gln Pro Gly Val Ala Met Pro Asn Leu Tyr Lys Met Gln 6805 6810 6815 Arg Met Leu Leu Glu Lys Cys Asp Leu Gln Asn Tyr Gly Asp Ser Ala 6820 6825 6830 Thr Leu Pro Lys Gly Ile Met Met Asn Val Ala Lys Tyr Thr Gln Leu 6835 6840 6845 Cys Gln Tyr Leu Asn Thr Leu Thr Leu Ala Val Pro Tyr Asn Met Arg 6850 6855 6860 Val Ile His Phe Gly Ala Gly Ser Asp Lys Gly Val Ala Pro Gly Thr 6865 6870 6875 6880 Ala Val Leu Arg Gln Trp Leu Pro Thr Gly Thr Leu Leu Val Asp Ser 6885 6890 6895 Asp Leu Asn Asp Phe Val Ser Asp Ala Asp Ser Thr Leu Ile Gly Asp 6900 6905 6910 Cys Ala Thr Val His Thr Ala Asn Lys Trp Asp Leu Ile Ile Ser Asp 6915 6920 6925 Met Tyr Asp Pro Lys Thr Lys Asn Val Thr Lys Glu Asn Asp Ser Lys 6930 6935 6940 Glu Gly Phe Phe Thr Tyr Ile Cys Gly Phe Ile Gln Gln Lys Leu Ala 6945 6950 6955 6960 Leu Gly Gly Ser Val Ala Ile Lys Ile Thr Glu His Ser Trp Asn Ala 6965 6970 6975 Asp Leu Tyr Lys Leu Met Gly His Phe Ala Trp Trp Thr Ala Phe Val 6980 6985 6990 Thr Asn Val Asn Ala Ser Ser Ser Glu Ala Phe Leu Ile Gly Cys Asn 6995 7000 7005 Tyr Leu Gly Lys Pro Arg Glu Gln Ile Asp Gly Tyr Val Met His Ala 7010 7015 7020 Asn Tyr Ile Phe Trp Arg Asn Thr Asn Pro Ile Gln Leu Ser Ser Tyr 7025 7030 7035 7040 Ser Leu Phe Asp Met Ser Lys Phe Pro Leu Lys Leu Arg Gly Thr Ala 7045 7050 7055 Val Met Ser Leu Lys Glu 7060 <210> 11 <211> 1273 <212> PRT <213> SARS-CoV2 <400> 11 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 225 230 235 240 Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 245 250 255 Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 260 265 270 Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 275 280 285 Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 290 295 300 Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 305 310 315 320 Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 325 330 335 Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 340 345 350 Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 355 360 365 Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 370 375 380 Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 385 390 395 400 Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 405 410 415 Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 420 425 430 Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 435 440 445 Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 450 455 460 Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 465 470 475 480 Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 485 490 495 Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 500 505 510 Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 515 520 525 Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 530 535 540 Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 545 550 555 560 Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 565 570 575 Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 580 585 590 Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 595 600 605 Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 610 615 620 His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 625 630 635 640 Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 645 650 655 Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 660 665 670 Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 675 680 685 Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 690 695 700 Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 705 710 715 720 Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 725 730 735 Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 740 745 750 Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 755 760 765 Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 770 775 780 Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 785 790 795 800 Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 805 810 815 Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 820 825 830 Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 835 840 845 Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 850 855 860 Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 865 870 875 880 Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 885 890 895 Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 900 905 910 Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 915 920 925 Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 930 935 940 Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 945 950 955 960 Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 965 970 975 Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 980 985 990 Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 995 1000 1005 Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 1010 1015 1020 Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 1025 1030 1035 1040 Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 1045 1050 1055 Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 1060 1065 1070 Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 1075 1080 1085 Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 1090 1095 1100 Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 1105 1110 1115 1120 Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 1125 1130 1135 Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 1140 1145 1150 Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 1155 1160 1165 Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 1170 1175 1180 Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 1185 1190 1195 1200 Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 1205 1210 1215 Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 1220 1225 1230 Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 1235 1240 1245 Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 1250 1255 1260 Leu Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 12 <211> 29874 <212> DNA <213> SARS-CoV2 <400> 12 attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 60 gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120 cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 180 ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240 cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 300 acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 360 agactccgtg gaggaggtct tatcagaggc acgtcaacat cttaaagatg gcacttgtgg 420 cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 480 acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 540 cgaaggcatt cagtacggtc gtagtggtga gacacttggt gtccttgtcc ctcatgtggg 600 cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 660 tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 720 tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 780 actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 840 ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 900 atgcactttg tccgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 960 tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1020 gacacctttt gaaattaaat tggcaaagaa atttgacacc ttcaatgggg aatgtccaaa 1080 ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1140 gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1200 caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1260 gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1320 aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1380 atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1440 cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc 1500 ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1560 ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 1620 aatactccaa aaagagaaag tcaacatcaa tattgttggt gactttaaac ttaatgaaga 1680 gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 1740 aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac 1800 aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1860 tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 1920 tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1980 aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 2040 taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg 2100 gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2160 agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 2220 ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcaa aggaaattaa 2280 ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 2340 tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca 2400 ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2460 tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 2520 aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2580 agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 2640 aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa caaacaatac 2700 cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 2760 agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2820 acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 2880 ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc 2940 actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3000 tgagtttaaa ttggcttcac atatgtattg ttctttctac cctccagatg aggatgaaga 3060 agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3120 agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 3180 agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3240 cggcagtgag gacaatcaga caactactat tcaaacaatt gttgaggttc aacctcaatt 3300 agagatggaa cttacaccag ttgttcagac tattgaagtg aatagtttta gtggttattt 3360 aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 3420 aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc 3480 aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3540 tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 3600 acactgtctt catgttgtcg gcccaaatgt taacaaaggt gaagacattc aacttcttaa 3660 gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 3720 tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa 3780 tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3840 aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa 3900 gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3960 caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 4020 cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4080 tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttgttca 4140 agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat 4200 gctagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4260 gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc 4320 cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4380 ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg 4440 tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4500 agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 4560 gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4620 tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 4680 agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc 4740 ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4800 agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga 4860 taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4920 ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4980 aacagtagac aacattaacc tccacacgca agttgtggac atgtcaatga catatggaca 5040 acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 5100 acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5160 tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5220 cactaaaaag tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa 5280 caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5340 acctgctcta caagatgctt attacagagc aagggctggt gaagctgcta acttttgtgc 5400 acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5460 gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 5520 taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5580 cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 5640 agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5700 tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 5760 gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt 5820 acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5880 ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 5940 tgaccctaag ttggacaatt attataagaa agacaattct tatttcacag agcaaccaat 6000 tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 6060 tgataatatc aaatttgctg atgatttaaa ccagttaact ggttataaga aacctgcttc 6120 aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6180 taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 6240 gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6300 tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 6360 cgcgcaggga atggataatc ttgcctgcga agatctaaaa ccagtctctg aagaagtagt 6420 ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 6480 aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6540 cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 6600 attatctaga gtattaggtt tgaaaaccct tgctactcat ggtttagctg ctgttaatag 6660 tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6720 aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 6780 ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc 6840 atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6900 ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttataatttg 6960 gtttttacta ttaagtgttt gcctaggttc tttaatctac tcaaccgctg ctttaggtgt 7020 tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7080 ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7140 tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7200 atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat 7260 tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7320 ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 7380 acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7440 tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 7500 ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag 7560 gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7620 tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 7680 cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7740 tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7800 ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7860 taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7920 atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 7980 agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8040 tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 8100 agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac 8160 ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8220 tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 8280 ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8340 tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8400 atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc 8460 tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8520 tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 8580 gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8640 tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8700 tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc 8760 tgattttgac acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc 8820 attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 8880 gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8940 tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9000 ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata 9060 ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9120 acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 9180 tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9240 agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 9300 atctttacca ggagttttct gtggtgtaga tgctgtaaat ttacttacta atatgtttac 9360 accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9420 tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 9480 tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9540 ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9600 gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9660 cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9720 tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 9780 tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa 9840 gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9900 taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 9960 tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10020 accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10080 atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg 10140 tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10200 gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 10260 ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10320 taaggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 10380 acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc 10440 tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10500 ttttaacata gattatgact gtgtctcttt ttgttacatg caccatatgg aattaccaac 10560 tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10620 aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 10680 cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga 10740 ctttaacctt gtggctatga agtacaatta tgaacctcta acacaagacc atgttgacat 10800 actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 10860 agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10920 tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 10980 gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt 11040 agttttagtc cagagtactc aatggtcttt gttctttttt ttgtatgaaa atgccttttt 11100 accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 11160 gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11220 ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11280 tagtttgtct ggttttaagc taaaagactg tgttatgtat gcatcagctg tagtgttact 11340 aatccttatg acagcaagaa ctgtgtatga tgatggtgct aggagagtgt ggacacttat 11400 gaatgtcttg acactcgttt ataaagttta ttatggtaat gctttagatc aagccatttc 11460 catgtgggct cttataatct ctgttacttc taactactca ggtgtagtta caactgtcat 11520 gtttttggcc agaggtattg tttttatgtg tgttgagtat tgccctattt tcttcataac 11580 tggtaataca cttcagtgta taatgctagt ttattgtttc ttaggctatt tttgtacttg 11640 ttactttggc ctcttttgtt tactcaaccg ctactttaga ctgactcttg gtgtttatga 11700 ttacttagtt tctacacagg agtttagata tatgaattca cagggactac tcccacccaa 11760 gaatagcata gatgccttca aactcaacat taaattgttg ggtgttggtg gcaaaccttg 11820 tatcaaagta gccactgtac agtctaaaat gtcagatgta aagtgcacat cagtagtctt 11880 actctcagtt ttgcaacaac tcagagtaga atcatcatct aaattgtggg ctcaatgtgt 11940 ccagttacac aatgacattc tcttagctaa agatactact gaagcctttg aaaaaatggt 12000 ttcactactt tctgttttgc tttccatgca gggtgctgta gacataaaca agctttgtga 12060 agaaatgctg gacaacaggg caaccttaca agctatagcc tcagagttta gttcccttcc 12120 atcatatgca gcttttgcta ctgctcaaga agcttatgag caggctgttg ctaatggtga 12180 ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat gtggctaaat ctgaatttga 12240 ccgtgatgca gccatgcaac gtaagttgga aaagatggct gatcaagcta tgacccaaat 12300 gtataaacag gctagatctg aggacaagag ggcaaaagtt actagtgcta tgcagacaat 12360 gcttttcact atgcttagaa agttggataa tgatgcactc aacaacatta tcaacaatgc 12420 aagagatggt tgtgttccct tgaacataat acctcttaca acagcagcca aactaatggt 12480 tgtcatacca gactataaca catataaaaa tacgtgtgat ggtacaacat ttacttatgc 12540 atcagcattg tgggaaatcc aacaggttgt agatgcagat agtaaaattg ttcaacttag 12600 tgaaattagt atggacaatt cacctaattt agcatggcct cttattgtaa cagctttaag 12660 ggccaattct gctgtcaaat tacagaataa tgagcttagt cctgttgcac tacgacagat 12720 gtcttgtgct gccggtacta cacaaactgc ttgcactgat gacaatgcgt tagcttacta 12780 caacacaaca aagggaggta ggtttgtact tgcactgtta tccgatttac aggatttgaa 12840 atgggctaga ttccctaaga gtgatggaac tggtactatc tatacagaac tggaaccacc 12900 ttgtaggttt gttacagaca cacctaaagg tcctaaagtg aagtatttat actttattaa 12960 aggattaaac aacctaaata gaggtatggt acttggtagt ttagctgcca cagtacgtct 13020 acaagctggt aatgcaacag aagtgcctgc caattcaact gtattatctt tctgtgcttt 13080 tgctgtagat gctgctaaag cttacaaaga ttatctagct agtgggggac aaccaatcac 13140 taattgtgtt aagatgttgt gtacacacac tggtactggt caggcaataa cagttacacc 13200 ggaagccaat atggatcaag aatcctttgg tggtgcatcg tgttgtctgt actgccgttg 13260 ccacatagat catccaaatc ctaaaggatt ttgtgactta aaaggtaagt atgtacaaat 13320 acctacaact tgtgctaatg accctgtggg ttttacactt aaaaacacag tctgtaccgt 13380 ctgcggtatg tggaaaggtt atggctgtag ttgtgatcaa ctccgcgaac ccatgcttca 13440 gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg taagtgcagc ccgtcttaca 13500 ccgtgcggca caggcactag tactgatgtc gtatacaggg cttttgacat ctacaatgat 13560 aaagtagctg gttttgctaa attcctaaaa actaattgtt gtcgcttcca agaaaaggac 13620 gaagatgaca atttaattga ttcttacttt gtagttaaga gacacacttt ctctaactac 13680 caacatgaag aaacaattta taatttactt aaggattgtc cagctgttgc taaacatgac 13740 ttctttaagt ttagaataga cggtgacatg gtaccacata tatcacgtca acgtcttact 13800 aaatacacaa tggcagacct cgtctatgct ttaaggcatt ttgatgaagg taattgtgac 13860 acattaaaag aaatacttgt cacatacaat tgttgtgatg atgattattt caataaaaag 13920 gactggtatg attttgtaga aaacccagat atattacgcg tatacgccaa cttaggtgaa 13980 cgtgtacgcc aagctttgtt aaaaacagta caattctgtg atgccatgcg aaatgctggt 14040 attgttggtg tactgacatt agataatcaa gatctcaatg gtaactggta tgatttcggt 14100 gatttcatac aaaccacgcc aggtagtgga gttcctgttg tagattctta ttattcattg 14160 ttaatgccta tattaacctt gaccagggct ttaactgcag agtcacatgt tgacactgac 14220 ttaacaaagc cttacattaa gtgggatttg ttaaaatatg acttcacgga agagaggtta 14280 aaactctttg accgttattt taaatattgg gatcagacat accacccaaa ttgtgttaac 14340 tgtttggatg acagatgcat tctgcattgt gcaaacttta atgttttatt ctctacagtg 14400 ttcccaccta caagttttgg accactagtg agaaaaatat ttgttgatgg tgttccattt 14460 gtagtttcaa ctggatacca cttcagagag ctaggtgttg tacataatca ggatgtaaac 14520 ttacatagct ctagacttag ttttaaggaa ttacttgtgt atgctgctga ccctgctatg 14580 cacgctgctt ctggtaatct attactagat aaacgcacta cgtgcttttc agtagctgca 14640 cttactaaca atgttgcttt tcaaactgtc aaacccggta attttaacaa agacttctat 14700 gactttgctg tgtctaaggg tttctttaag gaaggaagtt ctgttgaatt aaaacacttc 14760 ttctttgctc aggatggtaa tgctgctatc agcgattatg actactatcg ttataatcta 14820 ccaacaatgt gtgatatcag acaactacta tttgtagttg aagttgttga taagtacttt 14880 gattgttacg atggtggctg tattaatgct aaccaagtca tcgtcaacaa cctagacaaa 14940 tcagctggtt ttccatttaa taaatggggt aaggctagac tttattatga ttcaatgagt 15000 tatgaggatc aagatgcact tttcgcatat acaaaacgta atgtcatccc tactataact 15060 caaatgaatc ttaagtatgc cattagtgca aagaatagag ctcgcaccgt agctggtgtc 15120 tctatctgta gtactatgac caatagacag tttcatcaaa aattattgaa atcaatagcc 15180 gccactagag gagctactgt agtaattgga acaagcaaat tctatggtgg ttggcacaac 15240 atgttaaaaa ctgtttatag tgatgtagaa aaccctcacc ttatgggttg ggattatcct 15300 aaatgtgata gagccatgcc taacatgctt agaattatgg cctcacttgt tcttgctcgc 15360 aaacatacaa cgtgttgtag cttgtcacac cgtttctata gattagctaa tgagtgtgct 15420 caagtattga gtgaaatggt catgtgtggc ggttcactat atgttaaacc aggtggaacc 15480 tcatcaggag atgccacaac tgcttatgct aatagtgttt ttaacatttg tcaagctgtc 15540 acggccaatg ttaatgcact tttatctact gatggtaaca aaattgccga taagtatgtc 15600 cgcaatttac aacacagact ttatgagtgt ctctatagaa atagagatgt tgacacagac 15660 tttgtgaatg agttttacgc atatttgcgt aaacatttct caatgatgat actctctgac 15720 gatgctgttg tgtgtttcaa tagcacttat gcatctcaag gtctagtggc tagcataaag 15780 aactttaagt cagttcttta ttatcaaaac aatgttttta tgtctgaagc aaaatgttgg 15840 actgagactg accttactaa aggacctcat gaattttgct ctcaacatac aatgctagtt 15900 aaacagggtg atgattatgt gtaccttcct tacccagatc catcaagaat cctaggggcc 15960 ggctgttttg tagatgatat cgtaaaaaca gatggtacac ttatgattga acggttcgtg 16020 tctttagcta tagatgctta cccacttact aaacatccta atcaggagta tgctgatgtc 16080 tttcatttgt acttacaata cataagaaag ctacatgatg agttaacagg acacatgtta 16140 gacatgtatt ctgttatgct tactaatgat aacacttcaa ggtattggga acctgagttt 16200 tatgaggcta tgtacacacc gcatacagtc ttacaggctg ttggggcttg tgttctttgc 16260 aattcacaga cttcattaag atgtggtgct tgcatacgta gaccattctt atgttgtaaa 16320 tgctgttacg accatgtcat atcaacatca cataaattag tcttgtctgt taatccgtat 16380 gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc aactttactt aggaggtatg 16440 agctattatt gtaaatcaca taaaccaccc attagttttc cattgtgtgc taatggacaa 16500 gtttttggtt tatataaaaa tacatgtgtt ggtagcgata atgttactga ctttaatgca 16560 attgcaacat gtgactggac aaatgctggt gattacattt tagctaacac ctgtactgaa 16620 agactcaagc tttttgcagc agaaacgctc aaagctactg aggagacatt taaactgtct 16680 tatggtattg ctactgtacg tgaagtgctg tctgacagag aattacatct ttcatgggaa 16740 gttggtaaac ctagaccacc acttaaccga aattatgtct ttactggtta tcgtgtaact 16800 aaaaacagta aagtacaaat aggagagtac acctttgaaa aaggtgacta tggtgatgct 16860 gttgtttacc gaggtacaac aacttacaaa ttaaatgttg gtgattattt tgtgctgaca 16920 tcacatacag taatgccatt aagtgcacct acactagtgc cacaagagca ctatgttaga 16980 attactggct tatacccaac actcaatatc tcagatgagt tttctagcaa tgttgcaaat 17040 tatcaaaagg ttggtatgca aaagtattct acactccagg gaccacctgg tactggtaag 17100 agtcattttg ctattggcct agctctctac tacccttctg ctcgcatagt gtatacagct 17160 tgctctcatg ccgctgttga tgcactatgt gagaaggcat taaaatattt gcctatagat 17220 aaatgtagta gaattatacc tgcacgtgct cgtgtagagt gttttgataa attcaaagtg 17280 aattcaacat tagaacagta tgtcttttgt actgtaaatg cattgcctga gacgacagca 17340 gatatagttg tctttgatga aatttcaatg gccacaaatt atgatttgag tgttgtcaat 17400 gccagattac gtgctaagca ctatgtgtac attggcgacc ctgctcaatt acctgcacca 17460 cgcacattgc taactaaggg cacactagaa ccagaatatt tcaattcagt gtgtagactt 17520 atgaaaacta taggtccaga catgttcctc ggaacttgtc ggcgttgtcc tgctgaaatt 17580 gttgacactg tgagtgcttt ggtttatgat aataagctta aagcacataa agacaaatca 17640 gctcaatgct ttaaaatgtt ttataagggt gttatcacgc atgatgtttc atctgcaatt 17700 aacaggccac aaataggcgt ggtaagagaa ttccttacac gtaaccctgc ttggagaaaa 17760 gctgtcttta tttcacctta taattcacag aatgctgtag cctcaaagat tttgggacta 17820 ccaactcaaa ctgttgattc atcacagggc tcagaatatg actatgtcat attcactcaa 17880 accactgaaa cagctcactc ttgtaatgta aacagattta atgttgctat taccagagca 17940 aaagtaggca tactttgcat aatgtctgat agagaccttt atgacaagtt gcaatttaca 18000 agtcttgaaa ttccacgtag gaatgtggca actttacaag ctgaaaatgt aacaggactc 18060 tttaaagatt gtagtaaggt aatcactggg ttacatccta cacaggcacc tacacacctc 18120 agtgttgaca ctaaattcaa aactgaaggt ttatgtgttg acatacctgg catacctaag 18180 gacatgacct atagaagact catctctatg atgggtttta aaatgaatta tcaagttaat 18240 ggttacccta acatgtttat cacccgcgaa gaagctataa gacatgtacg tgcatggatt 18300 ggcttcgatg tcgaggggtg tcatgctact agagaagctg ttggtaccaa tttaccttta 18360 cagctaggtt tttctacagg tgttaaccta gttgctgtac ctacaggtta tgttgataca 18420 cctaataata cagatttttc cagagttagt gctaaaccac cgcctggaga tcaatttaaa 18480 cacctcatac cacttatgta caaaggactt ccttggaatg tagtgcgtat aaagattgta 18540 caaatgttaa gtgacacact taaaaatctc tctgacagag tcgtatttgt cttatgggca 18600 catggctttg agttgacatc tatgaagtat tttgtgaaaa taggacctga gcgcacctgt 18660 tgtctatgtg atagacgtgc cacatgcttt tccactgctt cagacactta tgcctgttgg 18720 catcattcta ttggatttga ttacgtctat aatccgttta tgattgatgt tcaacaatgg 18780 ggttttacag gtaacctaca aagcaaccat gatctgtatt gtcaagtcca tggtaatgca 18840 catgtagcta gttgtgatgc aatcatgact aggtgtctag ctgtccacga gtgctttgtt 18900 aagcgtgttg actggactat tgaatatcct ataattggtg atgaactgaa gattaatgcg 18960 gcttgtagaa aggttcaaca catggttgtt aaagctgcat tattagcaga caaattccca 19020 gttcttcacg acattggtaa ccctaaagct attaagtgtg tacctcaagc tgatgtagaa 19080 tggaagttct atgatgcaca gccttgtagt gacaaagctt ataaaataga agaattattc 19140 tattcttatg ccacacattc tgacaaattc acagatggtg tatgcctatt ttggaattgc 19200 aatgtcgata gatatcctgc taattccatt gtttgtagat ttgacactag agtgctatct 19260 aaccttaact tgcctggttg tgatggtggc agtttgtatg taaataaaca tgcattccac 19320 acaccagctt ttgataaaag tgcttttgtt aatttaaaac aattaccatt tttctattac 19380 tctgacagtc catgtgagtc tcatggaaaa caagtagtgt cagatataga ttatgtacca 19440 ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg gtgctgtctg tagacatcat 19500 gctaatgagt acagattgta tctcgatgct tataacatga tgatctcagc tggctttagc 19560 ttgtgggttt acaaacaatt tgatacttat aacctctgga acacttttac aagacttcag 19620 agtttagaaa atgtggcttt taatgttgta aataagggac actttgatgg acaacagggt 19680 gaagtaccag tttctatcat taataacact gtttacacaa aagttgatgg tgttgatgta 19740 gaattgtttg aaaataaaac aacattacct gttaatgtag catttgagct ttgggctaag 19800 cgcaacatta aaccagtacc agaggtgaaa atactcaata atttgggtgt ggacattgct 19860 gctaatactg tgatctggga ctacaaaaga gatgctccag cacatatatc tactattggt 19920 gtttgttcta tgactgacat agccaagaaa ccaactgaaa cgatttgtgc accactcact 19980 gtcttttttg atggtagagt tgatggtcaa gtagacttat ttagaaatgc ccgtaatggt 20040 gttcttatta cagaaggtag tgttaaaggt ttacaaccat ctgtaggtcc caaacaagct 20100 agtcttaatg gagtcacatt aattggagaa gccgtaaaaa cacagttcaa ttattataag 20160 aaagttgatg gtgttgtcca acaattacct gaaacttact ttactcagag tagaaattta 20220 caagaattta aacccaggag tcaaatggaa attgatttct tagaattagc tatggatgaa 20280 ttcattgaac ggtataaatt agaaggctat gccttcgaac atatcgttta tggagatttt 20340 agtcatagtc agttaggtgg tttacatcta ctgattggac tagctaaacg ttttaaggaa 20400 tcaccttttg aattagaaga ttttattcct atggacagta cagttaaaaa ctatttcata 20460 acagatgcgc aaacaggttc atctaagtgt gtgtgttctg ttattgattt attacttgat 20520 gattttgttg aaataataaa atcccaagat ttatctgtag tttctaaggt tgtcaaagtg 20580 actattgact atacagaaat ttcatttatg ctttggtgta aagatggcca tgtagaaaca 20640 ttttacccaa aattacaatc tagtcaagcg tggcaaccgg gtgttgctat gcctaatctt 20700 tacaaaatgc aaagaatgct attagaaaag tgtgaccttc aaaattatgg tgatagtgca 20760 acattaccta aaggcataat gatgaatgtc gcaaaatata ctcaactgtg tcaatattta 20820 aacacattaa cattagctgt accctataat atgagagtta tacattttgg tgctggttct 20880 gataaaggag ttgcaccagg tacagctgtt ttaagacagt ggttgcctac gggtacgctg 20940 cttgtcgatt cagatcttaa tgactttgtc tctgatgcag attcaacttt gattggtgat 21000 tgtgcaactg tacatacagc taataaatgg gatctcatta ttagtgatat gtacgaccct 21060 aagactaaaa atgttacaaa agaaaatgac tctaaagagg gttttttcac ttacatttgt 21120 gggtttatac aacaaaagct agctcttgga ggttccgtgg ctataaagat aacagaacat 21180 tcttggaatg ctgatcttta taagctcatg ggacacttcg catggtggac agcctttgtt 21240 actaatgtga atgcgtcatc atctgaagca tttttaattg gatgtaatta tcttggcaaa 21300 ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt acatattttg gaggaataca 21360 aatccaattc agttgtcttc ctattcttta tttgacatga gtaaatttcc ccttaaatta 21420 aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca atgatatgat tttatctctt 21480 cttagtaaag gtagacttat aattagagaa aacaacagag ttgttatttc tagtgatgtt 21540 cttgttaaca actaaacgaa caatgtttgt ttttcttgtt ttattgccac tagtctctag 21600 tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac 21660 acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga 21720 cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac 21780 caatggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc 21840 ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt tagattcgaa 21900 gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt 21960 tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat 22020 ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca 22080 gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt 22140 gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt 22200 gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat 22260 taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga 22320 ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag 22380 gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact 22440 tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta 22500 tcaaacttct aactttagag tccaaccaac agaatctatt gttagatttc ctaatattac 22560 aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg 22620 gaacaggaag agaatcagca actgtgttgc tgattattct ttcctatata attccgcatc 22680 attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac 22740 taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg 22800 gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt 22860 tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta 22920 tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta 22980 tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca 23040 atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact 23100 ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt 23160 ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac 23220 tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac 23280 tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg 23340 tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca 23400 ggatgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg 23460 gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc 23520 tgaacatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag 23580 ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat 23640 tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctaata actctattgc 23700 catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa 23760 gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt 23820 gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga 23880 acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc 23940 aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag 24000 caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt 24060 catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca 24120 aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata 24180 cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc 24240 attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca 24300 gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa 24360 aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa 24420 ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat 24480 ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat 24540 tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat 24600 tagagctgca gaaatcagag cttctgctaa tcttgctgct actaaaatgt cagagtgtgt 24660 acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc 24720 tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa 24780 gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg 24840 tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca 24900 aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt 24960 caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga 25020 taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa 25080 tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt 25140 aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc 25200 atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg tgacaattat 25260 gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg 25320 ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac 25380 ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt aactttgaag 25440 caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc aacgataccg 25500 atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct tgctgttttt 25560 cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact ctccaagggt 25620 gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca ccttttgctc 25680 gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta cttcttgcag 25740 agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg ccgttccaaa 25800 aacccattac tttatgatgc caactatttt ctttgctggc atactaattg ttacgactat 25860 tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga tggcacaaca 25920 agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg ggaatctgga 25980 gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca gctgtactca 26040 actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta caataaaatt 26100 gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acgtttcatc cggagttgtt 26160 aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt gcctttgtaa 26220 gcacaagctg atgagtacga acttatgtac tcattcgttt cggaagagac aggtacgtta 26280 atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt tacactagcc 26340 atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt gagtcttgta 26400 aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag agttcctgat 26460 cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact ttaattttag 26520 ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc cttgaacaat 26580 ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa tttgcctatg 26640 ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg ttatggccag 26700 taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc accggtggaa 26760 ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc attgcttctt 26820 tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc 26880 tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa 26940 tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta ggacgctgtg 27000 acatcaagga cctgcctaaa gaaatcactg ttgctacatc acgaacgctt tcttattaca 27060 aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac agtcgctaca 27120 ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat attgctttgc 27180 ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac tatagcagag 27240 atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga ttacatcata 27300 aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc tcaattagat 27360 gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt cttggcactg 27420 ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg tacaacagta 27480 cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt tcatcctcta 27540 gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc ttgtcctgac 27600 ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact gttcatcaga 27660 caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc aatagtgttt 27720 ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca ttaattgact 27780 tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt attatctttt 27840 ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg aacatgaaat 27900 ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa tgtagtttac 27960 agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt cacttctatt 28020 ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa ttgtgcgtgg 28080 atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat acagtttcct 28140 gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta gtgcgttgtt 28200 cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat ttcatctaaa 28260 cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac 28320 gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca gtggggcgcg 28380 atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt tcaccgctct 28440 cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc caattaacac 28500 caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac gaattcgtgg 28560 tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc taggaactgg 28620 gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg ttgcaactga 28680 gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta acaatgctgc 28740 aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg cagaagggag 28800 cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa 28860 ttcaactcca ggcagcagta ggggaacttc tcctgctaga atggctggca atggcggtga 28920 tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca aaatgtctgg 28980 taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa 29040 gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag 29100 acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca gacaaggaac 29160 tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag cgttcttcgg 29220 aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct acacaggtgc 29280 catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca 29340 tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc 29400 tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc 29460 tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc 29520 aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acgttttcgc 29580 ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc 29640 acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg tgtaacatta 29700 gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac gatcgagtgt 29760 acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg taaaattaat 29820 tttagtagtg ctatccccat gtgattttaa tagcttctta ggagaatgac aaaa 29874 <210> 13 <211> 4405 <212> PRT <213> SARS-CoV2 <400> 13 Met Glu Ser Leu Val Pro Gly Phe Asn Glu Lys Thr His Val Gln Leu 1 5 10 15 Ser Leu Pro Val Leu Gln Val Arg Asp Val Leu Val Arg Gly Phe Gly 20 25 30 Asp Ser Val Glu Glu Val Leu Ser Glu Ala Arg Gln His Leu Lys Asp 35 40 45 Gly Thr Cys Gly Leu Val Glu Val Glu Lys Gly Val Leu Pro Gln Leu 50 55 60 Glu Gln Pro Tyr Val Phe Ile Lys Arg Ser Asp Ala Arg Thr Ala Pro 65 70 75 80 His Gly His Val Met Val Glu Leu Val Ala Glu Leu Glu Gly Ile Gln 85 90 95 Tyr Gly Arg Ser Gly Glu Thr Leu Gly Val Leu Val Pro His Val Gly 100 105 110 Glu Ile Pro Val Ala Tyr Arg Lys Val Leu Leu Arg Lys Asn Gly Asn 115 120 125 Lys Gly Ala Gly Gly His Ser Tyr Gly Ala Asp Leu Lys Ser Phe Asp 130 135 140 Leu Gly Asp Glu Leu Gly Thr Asp Pro Tyr Glu Asp Phe Gln Glu Asn 145 150 155 160 Trp Asn Thr Lys His Ser Ser Gly Val Thr Arg Glu Leu Met Arg Glu 165 170 175 Leu Asn Gly Gly Ala Tyr Thr Arg Tyr Val Asp Asn Asn Phe Cys Gly 180 185 190 Pro Asp Gly Tyr Pro Leu Glu Cys Ile Lys Asp Leu Leu Ala Arg Ala 195 200 205 Gly Lys Ala Ser Cys Thr Leu Ser Glu Gln Leu Asp Phe Ile Asp Thr 210 215 220 Lys Arg Gly Val Tyr Cys Cys Arg Glu His Glu His Glu Ile Ala Trp 225 230 235 240 Tyr Thr Glu Arg Ser Glu Lys Ser Tyr Glu Leu Gln Thr Pro Phe Glu 245 250 255 Ile Lys Leu Ala Lys Lys Phe Asp Thr Phe Asn Gly Glu Cys Pro Asn 260 265 270 Phe Val Phe Pro Leu Asn Ser Ile Ile Lys Thr Ile Gln Pro Arg Val 275 280 285 Glu Lys Lys Lys Leu Asp Gly Phe Met Gly Arg Ile Arg Ser Val Tyr 290 295 300 Pro Val Ala Ser Pro Asn Glu Cys Asn Gln Met Cys Leu Ser Thr Leu 305 310 315 320 Met Lys Cys Asp His Cys Gly Glu Thr Ser Trp Gln Thr Gly Asp Phe 325 330 335 Val Lys Ala Thr Cys Glu Phe Cys Gly Thr Glu Asn Leu Thr Lys Glu 340 345 350 Gly Ala Thr Thr Cys Gly Tyr Leu Pro Gln Asn Ala Val Val Lys Ile 355 360 365 Tyr Cys Pro Ala Cys His Asn Ser Glu Val Gly Pro Glu His Ser Leu 370 375 380 Ala Glu Tyr His Asn Glu Ser Gly Leu Lys Thr Ile Leu Arg Lys Gly 385 390 395 400 Gly Arg Thr Ile Ala Phe Gly Gly Cys Val Phe Ser Tyr Val Gly Cys 405 410 415 His Asn Lys Cys Ala Tyr Trp Val Pro Arg Ala Ser Ala Asn Ile Gly 420 425 430 Cys Asn His Thr Gly Val Val Gly Glu Gly Ser Glu Gly Leu Asn Asp 435 440 445 Asn Leu Leu Glu Ile Leu Gln Lys Glu Lys Val Asn Ile Asn Ile Val 450 455 460 Gly Asp Phe Lys Leu Asn Glu Glu Ile Ala Ile Ile Leu Ala Ser Phe 465 470 475 480 Ser Ala Ser Thr Ser Ala Phe Val Glu Thr Val Lys Gly Leu Asp Tyr 485 490 495 Lys Ala Phe Lys Gln Ile Val Glu Ser Cys Gly Asn Phe Lys Val Thr 500 505 510 Lys Gly Lys Ala Lys Lys Gly Ala Trp Asn Ile Gly Glu Gln Lys Ser 515 520 525 Ile Leu Ser Pro Leu Tyr Ala Phe Ala Ser Glu Ala Ala Arg Val Val 530 535 540 Arg Ser Ile Phe Ser Arg Thr Leu Glu Thr Ala Gln Asn Ser Val Arg 545 550 555 560 Val Leu Gln Lys Ala Ala Ile Thr Ile Leu Asp Gly Ile Ser Gln Tyr 565 570 575 Ser Leu Arg Leu Ile Asp Ala Met Met Phe Thr Ser Asp Leu Ala Thr 580 585 590 Asn Asn Leu Val Val Met Ala Tyr Ile Thr Gly Gly Val Val Gln Leu 595 600 605 Thr Ser Gln Trp Leu Thr Asn Ile Phe Gly Thr Val Tyr Glu Lys Leu 610 615 620 Lys Pro Val Leu Asp Trp Leu Glu Glu Lys Phe Lys Glu Gly Val Glu 625 630 635 640 Phe Leu Arg Asp Gly Trp Glu Ile Val Lys Phe Ile Ser Thr Cys Ala 645 650 655 Cys Glu Ile Val Gly Gly Gln Ile Val Thr Cys Ala Lys Glu Ile Lys 660 665 670 Glu Ser Val Gln Thr Phe Phe Lys Leu Val Asn Lys Phe Leu Ala Leu 675 680 685 Cys Ala Asp Ser Ile Ile Ile Gly Gly Ala Lys Leu Lys Ala Leu Asn 690 695 700 Leu Gly Glu Thr Phe Val Thr His Ser Lys Gly Leu Tyr Arg Lys Cys 705 710 715 720 Val Lys Ser Arg Glu Glu Thr Gly Leu Leu Met Pro Leu Lys Ala Pro 725 730 735 Lys Glu Ile Ile Phe Leu Glu Gly Glu Thr Leu Pro Thr Glu Val Leu 740 745 750 Thr Glu Glu Val Val Leu Lys Thr Gly Asp Leu Gln Pro Leu Glu Gln 755 760 765 Pro Thr Ser Glu Ala Val Glu Ala Pro Leu Val Gly Thr Pro Val Cys 770 775 780 Ile Asn Gly Leu Met Leu Leu Glu Ile Lys Asp Thr Glu Lys Tyr Cys 785 790 795 800 Ala Leu Ala Pro Asn Met Met Val Thr Asn Asn Thr Phe Thr Leu Lys 805 810 815 Gly Gly Ala Pro Thr Lys Val Thr Phe Gly Asp Asp Thr Val Ile Glu 820 825 830 Val Gln Gly Tyr Lys Ser Val Asn Ile Thr Phe Glu Leu Asp Glu Arg 835 840 845 Ile Asp Lys Val Leu Asn Glu Lys Cys Ser Ala Tyr Thr Val Glu Leu 850 855 860 Gly Thr Glu Val Asn Glu Phe Ala Cys Val Val Ala Asp Ala Val Ile 865 870 875 880 Lys Thr Leu Gln Pro Val Ser Glu Leu Leu Thr Pro Leu Gly Ile Asp 885 890 895 Leu Asp Glu Trp Ser Met Ala Thr Tyr Tyr Leu Phe Asp Glu Ser Gly 900 905 910 Glu Phe Lys Leu Ala Ser His Met Tyr Cys Ser Phe Tyr Pro Pro Asp 915 920 925 Glu Asp Glu Glu Glu Gly Asp Cys Glu Glu Glu Glu Phe Glu Pro Ser 930 935 940 Thr Gln Tyr Glu Tyr Gly Thr Glu Asp Asp Tyr Gln Gly Lys Pro Leu 945 950 955 960 Glu Phe Gly Ala Thr Ser Ala Ala Leu Gln Pro Glu Glu Glu Gln Glu 965 970 975 Glu Asp Trp Leu Asp Asp Asp Ser Gln Gln Thr Val Gly Gln Gln Asp 980 985 990 Gly Ser Glu Asp Asn Gln Thr Thr Thr Ile Gln Thr Ile Val Glu Val 995 1000 1005 Gln Pro Gln Leu Glu Met Glu Leu Thr Pro Val Val Gln Thr Ile Glu 1010 1015 1020 Val Asn Ser Phe Ser Gly Tyr Leu Lys Leu Thr Asp Asn Val Tyr Ile 1025 1030 1035 1040 Lys Asn Ala Asp Ile Val Glu Glu Ala Lys Lys Val Lys Pro Thr Val 1045 1050 1055 Val Val Asn Ala Ala Asn Val Tyr Leu Lys His Gly Gly Gly Val Ala 1060 1065 1070 Gly Ala Leu Asn Lys Ala Thr Asn Asn Ala Met Gln Val Glu Ser Asp 1075 1080 1085 Asp Tyr Ile Ala Thr Asn Gly Pro Leu Lys Val Gly Gly Ser Cys Val 1090 1095 1100 Leu Ser Gly His Asn Leu Ala Lys His Cys Leu His Val Val Gly Pro 1105 1110 1115 1120 Asn Val Asn Lys Gly Glu Asp Ile Gln Leu Leu Lys Ser Ala Tyr Glu 1125 1130 1135 Asn Phe Asn Gln His Glu Val Leu Leu Ala Pro Leu Leu Ser Ala Gly 1140 1145 1150 Ile Phe Gly Ala Asp Pro Ile His Ser Leu Arg Val Cys Val Asp Thr 1155 1160 1165 Val Arg Thr Asn Val Tyr Leu Ala Val Phe Asp Lys Asn Leu Tyr Asp 1170 1175 1180 Lys Leu Val Ser Ser Phe Leu Glu Met Lys Ser Glu Lys Gln Val Glu 1185 1190 1195 1200 Gln Lys Ile Ala Glu Ile Pro Lys Glu Glu Val Lys Pro Phe Ile Thr 1205 1210 1215 Glu Ser Lys Pro Ser Val Glu Gln Arg Lys Gln Asp Asp Lys Lys Ile 1220 1225 1230 Lys Ala Cys Val Glu Glu Val Thr Thr Thr Leu Glu Glu Thr Lys Phe 1235 1240 1245 Leu Thr Glu Asn Leu Leu Leu Tyr Ile Asp Ile Asn Gly Asn Leu His 1250 1255 1260 Pro Asp Ser Ala Thr Leu Val Ser Asp Ile Asp Ile Thr Phe Leu Lys 1265 1270 1275 1280 Lys Asp Ala Pro Tyr Ile Val Gly Asp Val Val Gln Glu Gly Val Leu 1285 1290 1295 Thr Ala Val Val Ile Pro Thr Lys Lys Ala Gly Gly Thr Thr Glu Met 1300 1305 1310 Leu Ala Lys Ala Leu Arg Lys Val Pro Thr Asp Asn Tyr Ile Thr Thr 1315 1320 1325 Tyr Pro Gly Gln Gly Leu Asn Gly Tyr Thr Val Glu Glu Ala Lys Thr 1330 1335 1340 Val Leu Lys Lys Cys Lys Ser Ala Phe Tyr Ile Leu Pro Ser Ile Ile 1345 1350 1355 1360 Ser Asn Glu Lys Gln Glu Ile Leu Gly Thr Val Ser Trp Asn Leu Arg 1365 1370 1375 Glu Met Leu Ala His Ala Glu Glu Thr Arg Lys Leu Met Pro Val Cys 1380 1385 1390 Val Glu Thr Lys Ala Ile Val Ser Thr Ile Gln Arg Lys Tyr Lys Gly 1395 1400 1405 Ile Lys Ile Gln Glu Gly Val Val Asp Tyr Gly Ala Arg Phe Tyr Phe 1410 1415 1420 Tyr Thr Ser Lys Thr Thr Val Ala Ser Leu Ile Asn Thr Leu Asn Asp 1425 1430 1435 1440 Leu Asn Glu Thr Leu Val Thr Met Pro Leu Gly Tyr Val Thr His Gly 1445 1450 1455 Leu Asn Leu Glu Glu Ala Ala Arg Tyr Met Arg Ser Leu Lys Val Pro 1460 1465 1470 Ala Thr Val Ser Val Ser Ser Pro Asp Ala Val Thr Ala Tyr Asn Gly 1475 1480 1485 Tyr Leu Thr Ser Ser Ser Lys Thr Pro Glu Glu His Phe Ile Glu Thr 1490 1495 1500 Ile Ser Leu Ala Gly Ser Tyr Lys Asp Trp Ser Tyr Ser Gly Gln Ser 1505 1510 1515 1520 Thr Gln Leu Gly Ile Glu Phe Leu Lys Arg Gly Asp Lys Ser Val Tyr 1525 1530 1535 Tyr Thr Ser Asn Pro Thr Thr Phe His Leu Asp Gly Glu Val Ile Thr 1540 1545 1550 Phe Asp Asn Leu Lys Thr Leu Leu Ser Leu Arg Glu Val Arg Thr Ile 1555 1560 1565 Lys Val Phe Thr Thr Val Asp Asn Ile Asn Leu His Thr Gln Val Val 1570 1575 1580 Asp Met Ser Met Thr Tyr Gly Gln Gln Phe Gly Pro Thr Tyr Leu Asp 1585 1590 1595 1600 Gly Ala Asp Val Thr Lys Ile Lys Pro His Asn Ser His Glu Gly Lys 1605 1610 1615 Thr Phe Tyr Val Leu Pro Asn Asp Asp Thr Leu Arg Val Glu Ala Phe 1620 1625 1630 Glu Tyr Tyr His Thr Thr Asp Pro Ser Phe Leu Gly Arg Tyr Met Ser 1635 1640 1645 Ala Leu Asn His Thr Lys Lys Trp Lys Tyr Pro Gln Val Asn Gly Leu 1650 1655 1660 Thr Ser Ile Lys Trp Ala Asp Asn Asn Cys Tyr Leu Ala Thr Ala Leu 1665 1670 1675 1680 Leu Thr Leu Gln Gln Ile Glu Leu Lys Phe Asn Pro Pro Ala Leu Gln 1685 1690 1695 Asp Ala Tyr Tyr Arg Ala Arg Ala Gly Glu Ala Ala Asn Phe Cys Ala 1700 1705 1710 Leu Ile Leu Ala Tyr Cys Asn Lys Thr Val Gly Glu Leu Gly Asp Val 1715 1720 1725 Arg Glu Thr Met Ser Tyr Leu Phe Gln His Ala Asn Leu Asp Ser Cys 1730 1735 1740 Lys Arg Val Leu Asn Val Val Cys Lys Thr Cys Gly Gln Gln Gln Thr 1745 1750 1755 1760 Thr Leu Lys Gly Val Glu Ala Val Met Tyr Met Gly Thr Leu Ser Tyr 1765 1770 1775 Glu Gln Phe Lys Lys Gly Val Gln Ile Pro Cys Thr Cys Gly Lys Gln 1780 1785 1790 Ala Thr Lys Tyr Leu Val Gln Gln Glu Ser Pro Phe Val Met Met Ser 1795 1800 1805 Ala Pro Pro Ala Gln Tyr Glu Leu Lys His Gly Thr Phe Thr Cys Ala 1810 1815 1820 Ser Glu Tyr Thr Gly Asn Tyr Gln Cys Gly His Tyr Lys His Ile Thr 1825 1830 1835 1840 Ser Lys Glu Thr Leu Tyr Cys Ile Asp Gly Ala Leu Leu Thr Lys Ser 1845 1850 1855 Ser Glu Tyr Lys Gly Pro Ile Thr Asp Val Phe Tyr Lys Glu Asn Ser 1860 1865 1870 Tyr Thr Thr Thr Ile Lys Pro Val Thr Tyr Lys Leu Asp Gly Val Val 1875 1880 1885 Cys Thr Glu Ile Asp Pro Lys Leu Asp Asn Tyr Tyr Lys Lys Asp Asn 1890 1895 1900 Ser Tyr Phe Thr Glu Gln Pro Ile Asp Leu Val Pro Asn Gln Pro Tyr 1905 1910 1915 1920 Pro Asn Ala Ser Phe Asp Asn Phe Lys Phe Val Cys Asp Asn Ile Lys 1925 1930 1935 Phe Ala Asp Asp Leu Asn Gln Leu Thr Gly Tyr Lys Lys Pro Ala Ser 1940 1945 1950 Arg Glu Leu Lys Val Thr Phe Phe Pro Asp Leu Asn Gly Asp Val Val 1955 1960 1965 Ala Ile Asp Tyr Lys His Tyr Thr Pro Ser Phe Lys Lys Gly Ala Lys 1970 1975 1980 Leu Leu His Lys Pro Ile Val Trp His Val Asn Asn Ala Thr Asn Lys 1985 1990 1995 2000 Ala Thr Tyr Lys Pro Asn Thr Trp Cys Ile Arg Cys Leu Trp Ser Thr 2005 2010 2015 Lys Pro Val Glu Thr Ser Asn Ser Phe Asp Val Leu Lys Ser Glu Asp 2020 2025 2030 Ala Gln Gly Met Asp Asn Leu Ala Cys Glu Asp Leu Lys Pro Val Ser 2035 2040 2045 Glu Glu Val Val Glu Asn Pro Thr Ile Gln Lys Asp Val Leu Glu Cys 2050 2055 2060 Asn Val Lys Thr Thr Glu Val Val Gly Asp Ile Ile Leu Lys Pro Ala 2065 2070 2075 2080 Asn Asn Ser Leu Lys Ile Thr Glu Glu Val Gly His Thr Asp Leu Met 2085 2090 2095 Ala Ala Tyr Val Asp Asn Ser Ser Leu Thr Ile Lys Lys Pro Asn Glu 2100 2105 2110 Leu Ser Arg Val Leu Gly Leu Lys Thr Leu Ala Thr His Gly Leu Ala 2115 2120 2125 Ala Val Asn Ser Val Pro Trp Asp Thr Ile Ala Asn Tyr Ala Lys Pro 2130 2135 2140 Phe Leu Asn Lys Val Val Ser Thr Thr Thr Asn Ile Val Thr Arg Cys 2145 2150 2155 2160 Leu Asn Arg Val Cys Thr Asn Tyr Met Pro Tyr Phe Phe Thr Leu Leu 2165 2170 2175 Leu Gln Leu Cys Thr Phe Thr Arg Ser Thr Asn Ser Arg Ile Lys Ala 2180 2185 2190 Ser Met Pro Thr Thr Ile Ala Lys Asn Thr Val Lys Ser Val Gly Lys 2195 2200 2205 Phe Cys Leu Glu Ala Ser Phe Asn Tyr Leu Lys Ser Pro Asn Phe Ser 2210 2215 2220 Lys Leu Ile Asn Ile Ile Ile Trp Phe Leu Leu Leu Ser Val Cys Leu 2225 2230 2235 2240 Gly Ser Leu Ile Tyr Ser Thr Ala Ala Leu Gly Val Leu Met Ser Asn 2245 2250 2255 Leu Gly Met Pro Ser Tyr Cys Thr Gly Tyr Arg Glu Gly Tyr Leu Asn 2260 2265 2270 Ser Thr Asn Val Thr Ile Ala Thr Tyr Cys Thr Gly Ser Ile Pro Cys 2275 2280 2285 Ser Val Cys Leu Ser Gly Leu Asp Ser Leu Asp Thr Tyr Pro Ser Leu 2290 2295 2300 Glu Thr Ile Gln Ile Thr Ile Ser Ser Phe Lys Trp Asp Leu Thr Ala 2305 2310 2315 2320 Phe Gly Leu Val Ala Glu Trp Phe Leu Ala Tyr Ile Leu Phe Thr Arg 2325 2330 2335 Phe Phe Tyr Val Leu Gly Leu Ala Ala Ile Met Gln Leu Phe Phe Ser 2340 2345 2350 Tyr Phe Ala Val His Phe Ile Ser Asn Ser Trp Leu Met Trp Leu Ile 2355 2360 2365 Ile Asn Leu Val Gln Met Ala Pro Ile Ser Ala Met Val Arg Met Tyr 2370 2375 2380 Ile Phe Phe Ala Ser Phe Tyr Tyr Val Trp Lys Ser Tyr Val His Val 2385 2390 2395 2400 Val Asp Gly Cys Asn Ser Ser Thr Cys Met Met Cys Tyr Lys Arg Asn 2405 2410 2415 Arg Ala Thr Arg Val Glu Cys Thr Thr Ile Val Asn Gly Val Arg Arg 2420 2425 2430 Ser Phe Tyr Val Tyr Ala Asn Gly Gly Lys Gly Phe Cys Lys Leu His 2435 2440 2445 Asn Trp Asn Cys Val Asn Cys Asp Thr Phe Cys Ala Gly Ser Thr Phe 2450 2455 2460 Ile Ser Asp Glu Val Ala Arg Asp Leu Ser Leu Gln Phe Lys Arg Pro 2465 2470 2475 2480 Ile Asn Pro Thr Asp Gln Ser Ser Tyr Ile Val Asp Ser Val Thr Val 2485 2490 2495 Lys Asn Gly Ser Ile His Leu Tyr Phe Asp Lys Ala Gly Gln Lys Thr 2500 2505 2510 Tyr Glu Arg His Ser Leu Ser His Phe Val Asn Leu Asp Asn Leu Arg 2515 2520 2525 Ala Asn Asn Thr Lys Gly Ser Leu Pro Ile Asn Val Ile Val Phe Asp 2530 2535 2540 Gly Lys Ser Lys Cys Glu Glu Ser Ser Ala Lys Ser Ala Ser Val Tyr 2545 2550 2555 2560 Tyr Ser Gln Leu Met Cys Gln Pro Ile Leu Leu Leu Asp Gln Ala Leu 2565 2570 2575 Val Ser Asp Val Gly Asp Ser Ala Glu Val Ala Val Lys Met Phe Asp 2580 2585 2590 Ala Tyr Val Asn Thr Phe Ser Ser Thr Phe Asn Val Pro Met Glu Lys 2595 2600 2605 Leu Lys Thr Leu Val Ala Thr Ala Glu Ala Glu Leu Ala Lys Asn Val 2610 2615 2620 Ser Leu Asp Asn Val Leu Ser Thr Phe Ile Ser Ala Ala Arg Gln Gly 2625 2630 2635 2640 Phe Val Asp Ser Asp Val Glu Thr Lys Asp Val Val Glu Cys Leu Lys 2645 2650 2655 Leu Ser His Gln Ser Asp Ile Glu Val Thr Gly Asp Ser Cys Asn Asn 2660 2665 2670 Tyr Met Leu Thr Tyr Asn Lys Val Glu Asn Met Thr Pro Arg Asp Leu 2675 2680 2685 Gly Ala Cys Ile Asp Cys Ser Ala Arg His Ile Asn Ala Gln Val Ala 2690 2695 2700 Lys Ser His Asn Ile Ala Leu Ile Trp Asn Val Lys Asp Phe Met Ser 2705 2710 2715 2720 Leu Ser Glu Gln Leu Arg Lys Gln Ile Arg Ser Ala Ala Lys Lys Asn 2725 2730 2735 Asn Leu Pro Phe Lys Leu Thr Cys Ala Thr Thr Arg Gln Val Val Asn 2740 2745 2750 Val Val Thr Thr Lys Ile Ala Leu Lys Gly Gly Lys Ile Val Asn Asn 2755 2760 2765 Trp Leu Lys Gln Leu Ile Lys Val Thr Leu Val Phe Leu Phe Val Ala 2770 2775 2780 Ala Ile Phe Tyr Leu Ile Thr Pro Val His Val Met Ser Lys His Thr 2785 2790 2795 2800 Asp Phe Ser Ser Glu Ile Ile Gly Tyr Lys Ala Ile Asp Gly Gly Val 2805 2810 2815 Thr Arg Asp Ile Ala Ser Thr Asp Thr Cys Phe Ala Asn Lys His Ala 2820 2825 2830 Asp Phe Asp Thr Trp Phe Ser Gln Arg Gly Gly Ser Tyr Thr Asn Asp 2835 2840 2845 Lys Ala Cys Pro Leu Ile Ala Ala Val Ile Thr Arg Glu Val Gly Phe 2850 2855 2860 Val Val Pro Gly Leu Pro Gly Thr Ile Leu Arg Thr Thr Asn Gly Asp 2865 2870 2875 2880 Phe Leu His Phe Leu Pro Arg Val Phe Ser Ala Val Gly Asn Ile Cys 2885 2890 2895 Tyr Thr Pro Ser Lys Leu Ile Glu Tyr Thr Asp Phe Ala Thr Ser Ala 2900 2905 2910 Cys Val Leu Ala Ala Glu Cys Thr Ile Phe Lys Asp Ala Ser Gly Lys 2915 2920 2925 Pro Val Pro Tyr Cys Tyr Asp Thr Asn Val Leu Glu Gly Ser Val Ala 2930 2935 2940 Tyr Glu Ser Leu Arg Pro Asp Thr Arg Tyr Val Leu Met Asp Gly Ser 2945 2950 2955 2960 Ile Ile Gln Phe Pro Asn Thr Tyr Leu Glu Gly Ser Val Arg Val Val 2965 2970 2975 Thr Thr Phe Asp Ser Glu Tyr Cys Arg His Gly Thr Cys Glu Arg Ser 2980 2985 2990 Glu Ala Gly Val Cys Val Ser Thr Ser Gly Arg Trp Val Leu Asn Asn 2995 3000 3005 Asp Tyr Tyr Arg Ser Leu Pro Gly Val Phe Cys Gly Val Asp Ala Val 3010 3015 3020 Asn Leu Leu Thr Asn Met Phe Thr Pro Leu Ile Gln Pro Ile Gly Ala 3025 3030 3035 3040 Leu Asp Ile Ser Ala Ser Ile Val Ala Gly Gly Ile Val Ala Ile Val 3045 3050 3055 Val Thr Cys Leu Ala Tyr Tyr Phe Met Arg Phe Arg Arg Ala Phe Gly 3060 3065 3070 Glu Tyr Ser His Val Val Ala Phe Asn Thr Leu Leu Phe Leu Met Ser 3075 3080 3085 Phe Thr Val Leu Cys Leu Thr Pro Val Tyr Ser Phe Leu Pro Gly Val 3090 3095 3100 Tyr Ser Val Ile Tyr Leu Tyr Leu Thr Phe Tyr Leu Thr Asn Asp Val 3105 3110 3115 3120 Ser Phe Leu Ala His Ile Gln Trp Met Val Met Phe Thr Pro Leu Val 3125 3130 3135 Pro Phe Trp Ile Thr Ile Ala Tyr Ile Ile Cys Ile Ser Thr Lys His 3140 3145 3150 Phe Tyr Trp Phe Phe Ser Asn Tyr Leu Lys Arg Arg Val Val Phe Asn 3155 3160 3165 Gly Val Ser Phe Ser Thr Phe Glu Glu Ala Ala Leu Cys Thr Phe Leu 3170 3175 3180 Leu Asn Lys Glu Met Tyr Leu Lys Leu Arg Ser Asp Val Leu Leu Pro 3185 3190 3195 3200 Leu Thr Gln Tyr Asn Arg Tyr Leu Ala Leu Tyr Asn Lys Tyr Lys Tyr 3205 3210 3215 Phe Ser Gly Ala Met Asp Thr Thr Ser Tyr Arg Glu Ala Ala Cys Cys 3220 3225 3230 His Leu Ala Lys Ala Leu Asn Asp Phe Ser Asn Ser Gly Ser Asp Val 3235 3240 3245 Leu Tyr Gln Pro Pro Gln Thr Ser Ile Thr Ser Ala Val Leu Gln Ser 3250 3255 3260 Gly Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys Met 3265 3270 3275 3280 Val Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu Asp 3285 3290 3295 Asp Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp Met 3300 3305 3310 Leu Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His Asn 3315 3320 3325 Phe Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His Ser 3330 3335 3340 Met Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro Lys 3345 3350 3355 3360 Thr Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe Ser 3365 3370 3375 Val Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys Ala 3380 3385 3390 Met Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser Cys 3395 3400 3405 Gly Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys Tyr 3410 3415 3420 Met His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp Leu 3425 3430 3435 3440 Glu Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln Ala 3445 3450 3455 Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu Tyr 3460 3465 3470 Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr Thr 3475 3480 3485 Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu Pro 3490 3495 3500 Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln Thr 3505 3510 3515 3520 Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu Gln 3525 3530 3535 Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu Asp 3540 3545 3550 Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr Phe 3555 3560 3565 Gln Ser Ala Val Lys Arg Thr Ile Lys Gly Thr His His Trp Leu Leu 3570 3575 3580 Leu Thr Ile Leu Thr Ser Leu Leu Val Leu Val Gln Ser Thr Gln Trp 3585 3590 3595 3600 Ser Leu Phe Phe Phe Leu Tyr Glu Asn Ala Phe Leu Pro Phe Ala Met 3605 3610 3615 Gly Ile Ile Ala Met Ser Ala Phe Ala Met Met Phe Val Lys His Lys 3620 3625 3630 His Ala Phe Leu Cys Leu Phe Leu Leu Pro Ser Leu Ala Thr Val Ala 3635 3640 3645 Tyr Phe Asn Met Val Tyr Met Pro Ala Ser Trp Val Met Arg Ile Met 3650 3655 3660 Thr Trp Leu Asp Met Val Asp Thr Ser Leu Ser Gly Phe Lys Leu Lys 3665 3670 3675 3680 Asp Cys Val Met Tyr Ala Ser Ala Val Val Leu Leu Ile Leu Met Thr 3685 3690 3695 Ala Arg Thr Val Tyr Asp Asp Gly Ala Arg Arg Val Trp Thr Leu Met 3700 3705 3710 Asn Val Leu Thr Leu Val Tyr Lys Val Tyr Tyr Gly Asn Ala Leu Asp 3715 3720 3725 Gln Ala Ile Ser Met Trp Ala Leu Ile Ile Ser Val Thr Ser Asn Tyr 3730 3735 3740 Ser Gly Val Val Thr Thr Val Met Phe Leu Ala Arg Gly Ile Val Phe 3745 3750 3755 3760 Met Cys Val Glu Tyr Cys Pro Ile Phe Phe Ile Thr Gly Asn Thr Leu 3765 3770 3775 Gln Cys Ile Met Leu Val Tyr Cys Phe Leu Gly Tyr Phe Cys Thr Cys 3780 3785 3790 Tyr Phe Gly Leu Phe Cys Leu Leu Asn Arg Tyr Phe Arg Leu Thr Leu 3795 3800 3805 Gly Val Tyr Asp Tyr Leu Val Ser Thr Gln Glu Phe Arg Tyr Met Asn 3810 3815 3820 Ser Gln Gly Leu Leu Pro Pro Lys Asn Ser Ile Asp Ala Phe Lys Leu 3825 3830 3835 3840 Asn Ile Lys Leu Leu Gly Val Gly Gly Lys Pro Cys Ile Lys Val Ala 3845 3850 3855 Thr Val Gln Ser Lys Met Ser Asp Val Lys Cys Thr Ser Val Val Leu 3860 3865 3870 Leu Ser Val Leu Gln Gln Leu Arg Val Glu Ser Ser Ser Lys Leu Trp 3875 3880 3885 Ala Gln Cys Val Gln Leu His Asn Asp Ile Leu Leu Ala Lys Asp Thr 3890 3895 3900 Thr Glu Ala Phe Glu Lys Met Val Ser Leu Leu Ser Val Leu Leu Ser 3905 3910 3915 3920 Met Gln Gly Ala Val Asp Ile Asn Lys Leu Cys Glu Glu Met Leu Asp 3925 3930 3935 Asn Arg Ala Thr Leu Gln Ala Ile Ala Ser Glu Phe Ser Ser Leu Pro 3940 3945 3950 Ser Tyr Ala Ala Phe Ala Thr Ala Gln Glu Ala Tyr Glu Gln Ala Val 3955 3960 3965 Ala Asn Gly Asp Ser Glu Val Val Leu Lys Lys Leu Lys Lys Ser Leu 3970 3975 3980 Asn Val Ala Lys Ser Glu Phe Asp Arg Asp Ala Ala Met Gln Arg Lys 3985 3990 3995 4000 Leu Glu Lys Met Ala Asp Gln Ala Met Thr Gln Met Tyr Lys Gln Ala 4005 4010 4015 Arg Ser Glu Asp Lys Arg Ala Lys Val Thr Ser Ala Met Gln Thr Met 4020 4025 4030 Leu Phe Thr Met Leu Arg Lys Leu Asp Asn Asp Ala Leu Asn Asn Ile 4035 4040 4045 Ile Asn Asn Ala Arg Asp Gly Cys Val Pro Leu Asn Ile Ile Pro Leu 4050 4055 4060 Thr Thr Ala Ala Lys Leu Met Val Val Ile Pro Asp Tyr Asn Thr Tyr 4065 4070 4075 4080 Lys Asn Thr Cys Asp Gly Thr Thr Phe Thr Tyr Ala Ser Ala Leu Trp 4085 4090 4095 Glu Ile Gln Gln Val Val Asp Ala Asp Ser Lys Ile Val Gln Leu Ser 4100 4105 4110 Glu Ile Ser Met Asp Asn Ser Pro Asn Leu Ala Trp Pro Leu Ile Val 4115 4120 4125 Thr Ala Leu Arg Ala Asn Ser Ala Val Lys Leu Gln Asn Asn Glu Leu 4130 4135 4140 Ser Pro Val Ala Leu Arg Gln Met Ser Cys Ala Ala Gly Thr Thr Gln 4145 4150 4155 4160 Thr Ala Cys Thr Asp Asp Asn Ala Leu Ala Tyr Tyr Asn Thr Thr Lys 4165 4170 4175 Gly Gly Arg Phe Val Leu Ala Leu Leu Ser Asp Leu Gln Asp Leu Lys 4180 4185 4190 Trp Ala Arg Phe Pro Lys Ser Asp Gly Thr Gly Thr Ile Tyr Thr Glu 4195 4200 4205 Leu Glu Pro Pro Cys Arg Phe Val Thr Asp Thr Pro Lys Gly Pro Lys 4210 4215 4220 Val Lys Tyr Leu Tyr Phe Ile Lys Gly Leu Asn Asn Leu Asn Arg Gly 4225 4230 4235 4240 Met Val Leu Gly Ser Leu Ala Ala Thr Val Arg Leu Gln Ala Gly Asn 4245 4250 4255 Ala Thr Glu Val Pro Ala Asn Ser Thr Val Leu Ser Phe Cys Ala Phe 4260 4265 4270 Ala Val Asp Ala Ala Lys Ala Tyr Lys Asp Tyr Leu Ala Ser Gly Gly 4275 4280 4285 Gln Pro Ile Thr Asn Cys Val Lys Met Leu Cys Thr His Thr Gly Thr 4290 4295 4300 Gly Gln Ala Ile Thr Val Thr Pro Glu Ala Asn Met Asp Gln Glu Ser 4305 4310 4315 4320 Phe Gly Gly Ala Ser Cys Cys Leu Tyr Cys Arg Cys His Ile Asp His 4325 4330 4335 Pro Asn Pro Lys Gly Phe Cys Asp Leu Lys Gly Lys Tyr Val Gln Ile 4340 4345 4350 Pro Thr Thr Cys Ala Asn Asp Pro Val Gly Phe Thr Leu Lys Asn Thr 4355 4360 4365 Val Cys Thr Val Cys Gly Met Trp Lys Gly Tyr Gly Cys Ser Cys Asp 4370 4375 4380 Gln Leu Arg Glu Pro Met Leu Gln Ser Ala Asp Ala Gln Ser Phe Leu 4385 4390 4395 4400 Asn Gly Phe Ala Val 4405 <210> 14 <211> 1273 <212> PRT <213> SARS-CoV2 <400> 14 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 225 230 235 240 Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 245 250 255 Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 260 265 270 Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 275 280 285 Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 290 295 300 Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 305 310 315 320 Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 325 330 335 Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 340 345 350 Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Phe Leu 355 360 365 Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 370 375 380 Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 385 390 395 400 Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 405 410 415 Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 420 425 430 Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 435 440 445 Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 450 455 460 Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 465 470 475 480 Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 485 490 495 Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 500 505 510 Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 515 520 525 Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 530 535 540 Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 545 550 555 560 Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 565 570 575 Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 580 585 590 Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 595 600 605 Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 610 615 620 His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 625 630 635 640 Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 645 650 655 Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 660 665 670 Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 675 680 685 Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 690 695 700 Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 705 710 715 720 Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 725 730 735 Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 740 745 750 Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 755 760 765 Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 770 775 780 Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 785 790 795 800 Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 805 810 815 Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 820 825 830 Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 835 840 845 Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 850 855 860 Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 865 870 875 880 Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 885 890 895 Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 900 905 910 Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 915 920 925 Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 930 935 940 Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 945 950 955 960 Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 965 970 975 Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 980 985 990 Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 995 1000 1005 Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 1010 1015 1020 Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 1025 1030 1035 1040 Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 1045 1050 1055 Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 1060 1065 1070 Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 1075 1080 1085 Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 1090 1095 1100 Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 1105 1110 1115 1120 Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 1125 1130 1135 Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 1140 1145 1150 Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 1155 1160 1165 Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 1170 1175 1180 Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 1185 1190 1195 1200 Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 1205 1210 1215 Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 1220 1225 1230 Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 1235 1240 1245 Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 1250 1255 1260 Leu Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 15 <211> 29885 <212> DNA <213> SARS-CoV2 <220> <221> misc_feature <222> (1)..(30) <223> n is a, c, g, or t <220> <221> misc_feature <222> (29865) <223> n is a, c, g, or t <220> <221> misc_feature <222> (29872)..(29885) <223> n is a, c, g, or t <400> 15 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accaaccaac tttcgatctc ttgtagatct 60 gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120 cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 180 ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240 tgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 300 acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 360 agactccgtg gaggaggtct tatcagaggc acgtcaacat cttaaagatg gcacttgtgg 420 cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 480 acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 540 cgaaggcatt cagtacggtc gtagtggtga gacacttggt gtccttgtcc ctcatgtggg 600 cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 660 tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 720 tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 780 actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 840 ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 900 atgcactttg tccgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 960 tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1020 gacacctttt gaaattaaat tggcaaagaa atttgacacc ttcaatgggg aatgtccaaa 1080 ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1140 gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1200 caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1260 gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1320 aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1380 atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1440 cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc 1500 ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1560 ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 1620 aatactccaa aaagagaaag tcaacatcaa tattgttggt gactttaaac ttaatgaaga 1680 gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 1740 aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac 1800 aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1860 tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 1920 tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1980 aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 2040 taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg 2100 gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2160 agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 2220 ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcaa aggaaattaa 2280 ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 2340 tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca 2400 ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2460 tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 2520 aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2580 agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 2640 aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa caaacaatac 2700 cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 2760 agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2820 acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 2880 ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc 2940 actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3000 tgagtttaaa ttggcttcac atatgtattg ttctttttac cctccagatg aggatgaaga 3060 agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3120 agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 3180 agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3240 cggcagtgag gacaatcaga caactactat tcaaacaatt gttgaggttc aacctcaatt 3300 agagatggaa cttacaccag ttgttcagac tattgaagtg aatagtttta gtggttattt 3360 aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 3420 aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc 3480 aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3540 tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 3600 acactgtctt catgttgtcg gcccaaatgt taacaaaggt gaagacattc aacttcttaa 3660 gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 3720 tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa 3780 tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3840 aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa 3900 gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3960 caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 4020 cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4080 tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttgttca 4140 agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat 4200 gctagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4260 gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc 4320 cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4380 ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg 4440 tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4500 agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 4560 gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4620 tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 4680 agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc 4740 ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4800 agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga 4860 taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4920 ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4980 aacagtagac aacattaacc tccacacgca agttgtggac atgtcaatga catatggaca 5040 acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 5100 acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5160 tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5220 cactaaaaag tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa 5280 caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5340 acctgctcta caagatgctt attacagagc aagggctggt gaagctgcta acttttgtgc 5400 acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5460 gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 5520 taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5580 cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 5640 agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5700 tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 5760 gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt 5820 acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5880 ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 5940 tgaccctaag ttggacaatt attataagaa agacaattct tatttcacag agcaaccaat 6000 tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 6060 tgataatatc aaatttgctg atgatttaaa ccagttaact ggttataaga aacctgcttc 6120 aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6180 taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 6240 gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6300 tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 6360 cgcgcaggga atggataatc ttgcctgcga agatctaaaa ccagtctctg aagaagtagt 6420 ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 6480 aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6540 cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 6600 attatctaga gtattaggtt tgaaaaccct tgctactcat ggtttagctg ctgttaatag 6660 tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6720 aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 6780 ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc 6840 atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6900 ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttataatttg 6960 gtttttacta ttaagtgttt gcctaggttc tttaatctac tcaaccgctg ctttaggtgt 7020 tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7080 ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7140 tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7200 atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat 7260 tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7320 ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 7380 acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7440 tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 7500 ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag 7560 gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7620 tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 7680 cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7740 tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7800 ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7860 taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7920 atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 7980 agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8040 tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 8100 agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac 8160 ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8220 tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 8280 ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8340 tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8400 atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc 8460 tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8520 tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 8580 gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8640 tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8700 tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc 8760 tgattttgac acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc 8820 attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 8880 gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8940 tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9000 ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata 9060 ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9120 acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 9180 tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9240 agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 9300 atctttacca ggagttttct gtggtgtaga tgctgtaaat ttacttacta atatgtttac 9360 accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9420 tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 9480 tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9540 ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9600 gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9660 cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9720 tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 9780 tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa 9840 gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9900 taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 9960 tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10020 accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10080 atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg 10140 tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10200 gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 10260 ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10320 taaggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 10380 acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc 10440 tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10500 ttttaacata gattatgact gtgtctcttt ttgttacatg caccatatgg aattaccaac 10560 tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10620 aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 10680 cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga 10740 ctttaacctt gtggctatga agtacaatta tgaacctcta acacaagacc atgttgacat 10800 actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 10860 agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10920 tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 10980 gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt 11040 agttttagtc cagagtactc aatggtcttt gttctttttt ttgtatgaaa atgccttttt 11100 accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 11160 gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11220 ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11280 tagtttgtct ggttttaagc taaaagactg tgttatgtat gcatcagctg tagtgttact 11340 aatccttatg acagcaagaa ctgtgtatga tgatggtgct aggagagtgt ggacacttat 11400 gaatgtcttg acactcgttt ataaagttta ttatggtaat gctttagatc aagccatttc 11460 catgtgggct cttataatct ctgttacttc taactactca ggtgtagtta caactgtcat 11520 gtttttggcc agaggtattg tttttatgtg tgttgagtat tgccctattt tcttcataac 11580 tggtaataca cttcagtgta taatgctagt ttattgtttc ttaggctatt tttgtacttg 11640 ttactttggc ctcttttgtt tactcaaccg ctactttaga ctgactcttg gtgtttatga 11700 ttacttagtt tctacacagg agtttagata tatgaattca cagggactac tcccacccaa 11760 gaatagcata gatgccttca aactcaacat taaattgttg ggtgttggtg gcaaaccttg 11820 tatcaaagta gccactgtac agtctaaaat gtcagatgta aagtgcacat cagtagtctt 11880 actctcagtt ttgcaacaac tcagagtaga atcatcatct aaattgtggg ctcaatgtgt 11940 ccagttacac aatgacattc tcttagctaa agatactact gaagcctttg aaaaaatggt 12000 ttcactactt tctgttttgc tttccatgca gggtgctgta gacataaaca agctttgtga 12060 agaaatgctg gacaacaggg caaccttaca agctatagcc tcagagttta gttcccttcc 12120 atcatatgca gcttttgcta ctgctcaaga agcttatgag caggctgttg ctaatggtga 12180 ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat gtggctaaat ctgaatttga 12240 ccgtgatgca gccatgcaac gtaagttgga aaagatggct gatcaagcta tgacccaaat 12300 gtataaacag gctagatctg aggacaagag ggcaaaagtt actagtgcta tgcagacaat 12360 gcttttcact atgcttagaa agttggataa tgatgcactc aacaacatta tcaacaatgc 12420 aagagatggt tgtgttccct tgaacataat acctcttaca acagcagcca aactaatggt 12480 tgtcatacca gactataaca catataaaaa tacgtgtgat ggtacaacat ttacttatgc 12540 atcagcattg tgggaaatcc aacaggttgt agatgcagat agtaaaattg ttcaacttag 12600 tgaaattagt atggacaatt cacctaattt agcatggcct cttattgtaa cagctttaag 12660 ggccaattct gctgtcaaat tacagaataa tgagcttagt cctgttgcac tacgacagat 12720 gtcttgtgct gccggtacta cacaaactgc ttgcactgat gacaatgcgt tagcttacta 12780 caacacaaca aagggaggta ggtttgtact tgcactgtta tccgatttac aggatttgaa 12840 atgggctaga ttccctaaga gtgatggaac tggtactatc tatacagaac tggaaccacc 12900 ttgtaggttt gttacagaca cacctaaagg tcctaaagtg aagtatttat actttattaa 12960 aggattaaac aacctaaata gaggtatggt acttggtagt ttagctgcca cagtacgtct 13020 acaagctggt aatgcaacag aagtgcctgc caattcaact gtattatctt tctgtgcttt 13080 tgctgtagat gctgctaaag cttacaaaga ttatctagct agtgggggac aaccaatcac 13140 taattgtgtt aagatgttgt gtacacacac tggtactggt caggcaataa cagttacacc 13200 ggaagccaat atggatcaag aatcctttgg tggtgcatcg tgttgtctgt actgccgttg 13260 ccacatagat catccaaatc ctaaaggatt ttgtgactta aaaggtaagt atgtacaaat 13320 acctacaact tgtgctaatg accctgtggg ttttacactt aaaaacacag tctgtaccgt 13380 ctgcggtatg tggaaaggtt atggctgtag ttgtgatcaa ctccgcgaac ccatgcttca 13440 gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg taagtgcagc ccgtcttaca 13500 ccgtgcggca caggcactag tactgatgtc gtatacaggg cttttgacat ctacaatgat 13560 aaagtagctg gttttgctaa attcctaaaa actaattgtt gtcgcttcca agaaaaggac 13620 gaagatgaca atttaattga ttcttacttt gtagttaaga gacacacttt ctctaactac 13680 caacatgaag aaacaattta taatttactt aaggattgtc cagctgttgc taaacatgac 13740 ttctttaagt ttagaataga cggtgacatg gtaccacata tatcacgtca acgtcttact 13800 aaatacacaa tggcagacct cgtctatgct ttaaggcatt ttgatgaagg taattgtgac 13860 acattaaaag aaatacttgt cacatacaat tgttgtgatg atgattattt caataaaaag 13920 gactggtatg attttgtaga aaacccagat atattacgcg tatacgccaa cttaggtgaa 13980 cgtgtacgcc aagctttgtt aaaaacagta caattctgtg atgccatgcg aaatgctggt 14040 attgttggtg tactgacatt agataatcaa gatctcaatg gtaactggta tgatttcggt 14100 gatttcatac aaaccacgcc aggtagtgga gttcctgttg tagattctta ttattcattg 14160 ttaatgccta tattaacctt gaccagggct ttaactgcag agtcacatgt tgacactgac 14220 ttaacaaagc cttacattaa gtgggatttg ttaaaatatg acttcacgga agagaggtta 14280 aaactctttg accgttattt taaatattgg gatcagacat accacccaaa ttgtgttaac 14340 tgtttggatg acagatgcat tctgcattgt gcaaacttta atgttttatt ctctacagtg 14400 ttcccactta caagttttgg accactagtg agaaaaatat ttgttgatgg tgttccattt 14460 gtagtttcaa ctggatacca cttcagagag ctaggtgttg tacataatca ggatgtaaac 14520 ttacatagct ctagacttag ttttaaggaa ttacttgtgt atgctgctga ccctgctatg 14580 cacgctgctt ctggtaatct attactagat aaacgcacta cgtgcttttc agtagctgca 14640 cttactaaca atgttgcttt tcaaactgtc aaacccggta attttaacaa agacttctat 14700 gactttgctg tgtctaaggg tttctttaag gaaggaagtt ctgttgaatt aaaacacttc 14760 ttctttgctc aggatggtaa tgctgctatc agcgattatg actactatcg ttataatcta 14820 ccaacaatgt gtgatatcag acaactacta tttgtagttg aagttgttga taagtacttt 14880 gattgttacg atggtggctg tattaatgct aaccaagtca tcgtcaacaa cctagacaaa 14940 tcagctggtt ttccatttaa taaatggggt aaggctagac tttattatga ttcaatgagt 15000 tatgaggatc aagatgcact tttcgcatat acaaaacgta atgtcatccc tactataact 15060 caaatgaatc ttaagtatgc cattagtgca aagaatagag ctcgcaccgt agctggtgtc 15120 tctatctgta gtactatgac caatagacag tttcatcaaa aattattgaa atcaatagcc 15180 gccactagag gagctactgt agtaattgga acaagcaaat tctatggtgg ttggcacaac 15240 atgttaaaaa ctgtttatag tgatgtagaa aaccctcacc ttatgggttg ggattatcct 15300 aaatgtgata gagccatgcc taacatgctt agaattatgg cctcacttgt tcttgctcgc 15360 aaacatacaa cgtgttgtag cttgtcacac cgtttctata gattagctaa tgagtgtgct 15420 caagtattga gtgaaatggt catgtgtggc ggttcactat atgttaaacc aggtggaacc 15480 tcatcaggag atgccacaac tgcttatgct aatagtgttt ttaacatttg tcaagctgtc 15540 acggccaatg ttaatgcact tttatctact gatggtaaca aaattgccga taagtatgtc 15600 cgcaatttac aacacagact ttatgagtgt ctctatagaa atagagatgt tgacacagac 15660 tttgtgaatg agttttacgc atatttgcgt aaacatttct caatgatgat actctctgac 15720 gatgctgttg tgtgtttcaa tagcacttat gcatctcaag gtctagtggc tagcataaag 15780 aactttaagt cagttcttta ttatcaaaac aatgttttta tgtctgaagc aaaatgttgg 15840 actgagactg accttactaa aggacctcat gaattttgct ctcaacatac aatgctagtt 15900 aaacagggtg atgattatgt gtaccttcct tacccagatc catcaagaat cctaggggcc 15960 ggctgttttg tagatgatat cgtaaaaaca gatggtacac ttatgattga acggttcgtg 16020 tctttagcta tagatgctta cccacttact aaacatccta atcaggagta tgctgatgtc 16080 tttcatttgt acttacaata cataagaaag ctacatgatg agttaacagg acacatgtta 16140 gacatgtatt ctgttatgct tactaatgat aacacttcaa ggtattggga acctgagttt 16200 tatgaggcta tgtacacacc gcatacagtc ttacaggctg ttggggcttg tgttctttgc 16260 aattcacaga cttcattaag atgtggtgct tgcatacgta gaccattctt atgttgtaaa 16320 tgctgttacg accatgtcat atcaacatca cataaattag tcttgtctgt taatccgtat 16380 gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc aactttactt aggaggtatg 16440 agctattatt gtaaatcaca taaaccaccc attagttttc cattgtgtgc taatggacaa 16500 gtttttggtt tatataaaaa tacatgtgtt ggtagcgata atgttactga ctttaatgca 16560 attgcaacat gtgactggac aaatgctggt gattacattt tagctaacac ctgtactgaa 16620 agactcaagc tttttgcagc agaaacgctc aaagctactg aggagacatt taaactgtct 16680 tatggtattg ctactgtacg tgaagtgctg tctgacagag aattacatct ttcatgggaa 16740 gttggtaaac ctagaccacc acttaaccga aattatgtct ttactggtta tcgtgtaact 16800 aaaaacagta aagtacaaat aggagagtac acctttgaaa aaggtgacta tggtgatgct 16860 gttgtttacc gaggtacaac aacttacaaa ttaaatgttg gtgattattt tgtgctgaca 16920 tcacatacag taatgccatt aagtgcacct acactagtgc cacaagagca ctatgttaga 16980 attactggct tatacccaac actcaatatc tcagatgagt tttctagcaa tgttgcaaat 17040 tatcaaaagg ttggtatgca aaagtattct acactccagg gaccacctgg tactggtaag 17100 agtcattttg ctattggcct agctctctac tacccttctg ctcgcatagt gtatacagct 17160 tgctctcatg ccgctgttga tgcactatgt gagaaggcat taaaatattt gcctatagat 17220 aaatgtagta gaattatacc tgcacgtgct cgtgtagagt gttttgataa attcaaagtg 17280 aattcaacat tagaacagta tgtcttttgt actgtaaatg cattgcctga gacgacagca 17340 gatatagttg tctttgatga aatttcaatg gccacaaatt atgatttgag tgttgtcaat 17400 gccagattac gtgctaagca ctatgtgtac attggcgacc ctgctcaatt acctgcacca 17460 cgcacattgc taactaaggg cacactagaa ccagaatatt tcaattcagt gtgtagactt 17520 atgaaaacta taggtccaga catgttcctc ggaacttgtc ggcgttgtcc tgctgaaatt 17580 gttgacactg tgagtgcttt ggtttatgat aataagctta aagcacataa agacaaatca 17640 gctcaatgct ttaaaatgtt ttataagggt gttatcacgc atgatgtttc atctgcaatt 17700 aacaggccac aaataggcgt ggtaagagaa ttccttacac gtaaccctgc ttggagaaaa 17760 gctgtcttta tttcacctta taattcacag aatgctgtag cctcaaagat tttgggacta 17820 ccaactcaaa ctgttgattc atcacagggc tcagaatatg actatgtcat attcactcaa 17880 accactgaaa cagctcactc ttgtaatgta aacagattta atgttgctat taccagagca 17940 aaagtaggca tactttgcat aatgtctgat agagaccttt atgacaagtt gcaatttaca 18000 agtcttgaaa ttccacgtag gaatgtggca actttacaag ctgaaaatgt aacaggactc 18060 tttaaagatt gtagtaaggt aatcactggg ttacatccta cacaggcacc tacacacctc 18120 agtgttgaca ctaaattcaa aactgaaggt ttatgtgttg acatacctgg catacctaag 18180 gacatgacct atagaagact catctctatg atgggtttta aaatgaatta tcaagttaat 18240 ggttacccta acatgtttat cacccgcgaa gaagctataa gacatgtacg tgcatggatt 18300 ggcttcgatg tcgaggggtg tcatgctact agagaagctg ttggtaccaa tttaccttta 18360 cagctaggtt tttctacagg tgttaaccta gttgctgtac ctacaggtta tgttgataca 18420 cctaataata cagatttttc cagagttagt gctaaaccac cgcctggaga tcaatttaaa 18480 cacctcatac cacttatgta caaaggactt ccttggaatg tagtgcgtat aaagattgta 18540 caaatgttaa gtgacacact taaaaatctc tctgacagag tcgtatttgt cttatgggca 18600 catggctttg agttgacatc tatgaagtat tttgtgaaaa taggacctga gcgcacctgt 18660 tgtctatgtg atagacgtgc cacatgcttt tccactgctt cagacactta tgcctgttgg 18720 catcattcta ttggatttga ttacgtctat aatccgttta tgattgatgt tcaacaatgg 18780 ggttttacag gtaacctaca aagcaaccat gatctgtatt gtcaagtcca tggtaatgca 18840 catgtagcta gttgtgatgc aatcatgact aggtgtctag ctgtccacga gtgctttgtt 18900 aagcgtgttg actggactat tgaatatcct ataattggtg atgaactgaa gattaatgcg 18960 gcttgtagaa aggttcaaca catggttgtt aaagctgcat tattagcaga caaattccca 19020 gttcttcacg acattggtaa ccctaaagct attaagtgtg tacctcaagc tgatgtagaa 19080 tggaagttct atgatgcaca gccttgtagt gacaaagctt ataaaataga agaattattc 19140 tattcttatg ccacacattc tgacaaattc acagatggtg tatgcctatt ttggaattgc 19200 aatgtcgata gatatcctgc taattccatt gtttgtagat ttgacactag agtgctatct 19260 aaccttaact tgcctggttg tgatggtggc agtttgtatg taaataaaca tgcattccac 19320 acaccagctt ttgataaaag tgcttttgtt aatttaaaac aattaccatt tttctattac 19380 tctgacagtc catgtgagtc tcatggaaaa caagtagtgt cagatataga ttatgtacca 19440 ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg gtgctgtctg tagacatcat 19500 gctaatgagt acagattgta tctcgatgct tataacatga tgatctcagc tggctttagc 19560 ttgtgggttt acaaacaatt tgatacttat aacctctgga acacttttac aagacttcag 19620 agtttagaaa atgtggcttt taatgttgta aataagggac actttgatgg acaacagggt 19680 gaagtaccag tttctatcat taataacact gtttacacaa aagttgatgg tgttgatgta 19740 gaattgtttg aaaataaaac aacattacct gttaatgtag catttgagct ttgggctaag 19800 cgcaacatta aaccagtacc agaggtgaaa atactcaata atttgggtgt ggacattgct 19860 gctaatactg tgatctggga ctacaaaaga gatgctccag cacatatatc tactattggt 19920 gtttgttcta tgactgacat agccaagaaa ccaactgaaa cgatttgtgc accactcact 19980 gtcttttttg atggtagagt tgatggtcaa gtagacttat ttagaaatgc ccgtaatggt 20040 gttcttatta cagaaggtag tgttaaaggt ttacaaccat ctgtaggtcc caaacaagct 20100 agtcttaatg gagtcacatt aattggagaa gccgtaaaaa cacagttcaa ttattataag 20160 aaagttgatg gtgttgtcca acaattacct gaaacttact ttactcagag tagaaattta 20220 caagaattta aacccaggag tcaaatggaa attgatttct tagaattagc tatggatgaa 20280 ttcattgaac ggtataaatt agaaggctat gccttcgaac atatcgttta tggagatttt 20340 agtcatagtc agttaggtgg tttacatcta ctgattggac tagctaaacg ttttaaggaa 20400 tcaccttttg aattagaaga ttttattcct atggacagta cagttaaaaa ctatttcata 20460 acagatgcgc aaacaggttc atctaagtgt gtgtgttctg ttattgattt attacttgat 20520 gattttgttg aaataataaa atcccaagat ttatctgtag tttctaaggt tgtcaaagtg 20580 actattgact atacagaaat ttcatttatg ctttggtgta aagatggcca tgtagaaaca 20640 ttttacccaa aattacaatc tagtcaagcg tggcaaccgg gtgttgctat gcctaatctt 20700 tacaaaatgc aaagaatgct attagaaaag tgtgaccttc aaaattatgg tgatagtgca 20760 acattaccta aaggcataat gatgaatgtc gcaaaatata ctcaactgtg tcaatattta 20820 aacacattaa cattagctgt accctataat atgagagtta tacattttgg tgctggttct 20880 gataaaggag ttgcaccagg tacagctgtt ttaagacagt ggttgcctac gggtacgctg 20940 cttgtcgatt cagatcttaa tgactttgtc tctgatgcag attcaacttt gattggtgat 21000 tgtgcaactg tacatacagc taataaatgg gatctcatta ttagtgatat gtacgaccct 21060 aagactaaaa atgttacaaa agaaaatgac tctaaagagg gttttttcac ttacatttgt 21120 gggtttatac aacaaaagct agctcttgga ggttccgtgg ctataaagat aacagaacat 21180 tcttggaatg ctgatcttta taagctcatg ggacacttcg catggtggac agcctttgtt 21240 actaatgtga atgcgtcatc atctgaagca tttttaattg gatgtaatta tcttggcaaa 21300 ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt acatattttg gaggaataca 21360 aatccaattc agttgtcttc ctattcttta tttgacatga gtaaatttcc ccttaaatta 21420 aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca atgatatgat tttatctctt 21480 cttagtaaag gtagacttat aattagagaa aacaacagag ttgttatttc tagtgatgtt 21540 cttgttaaca actaaacgaa caatgtttgt ttttcttgtt ttattgccac tagtctctag 21600 tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac 21660 acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga 21720 cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac 21780 caatggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc 21840 ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt tagattcgaa 21900 gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt 21960 tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat 22020 ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca 22080 gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt 22140 gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt 22200 gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat 22260 taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga 22320 ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag 22380 gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact 22440 tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta 22500 tcaaacttct aactttagag kccaaccaac agaatctatt gttagatttc ctaatattac 22560 aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg 22620 gaacaggaag agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc 22680 attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac 22740 taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg 22800 gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt 22860 tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta 22920 tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta 22980 tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca 23040 atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact 23100 ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt 23160 ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac 23220 tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac 23280 tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg 23340 tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca 23400 gggtgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg 23460 gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc 23520 tgaacatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag 23580 ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat 23640 tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctrata actctattgc 23700 catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa 23760 gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt 23820 gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga 23880 acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc 23940 aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag 24000 caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt 24060 catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca 24120 aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata 24180 cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc 24240 attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca 24300 gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa 24360 aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa 24420 ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat 24480 ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat 24540 tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat 24600 tagagctgca gaaatcagag tttctgctaa tcttgctgct actaaaatgt cagagtgtgt 24660 acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc 24720 tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa 24780 gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg 24840 tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca 24900 aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt 24960 caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga 25020 taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa 25080 tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt 25140 aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc 25200 atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg tgacaattat 25260 gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg 25320 ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac 25380 ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt aactttgaag 25440 caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc aacgataccg 25500 atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct tgctgttttt 25560 cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact ctccaagggt 25620 gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca ccttttgctc 25680 gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta cttcttgcag 25740 agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg ccgttccaaa 25800 aacccattac tttatgatgc caactatttt ctttgctggc atactaattg ttacgactat 25860 tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga tggcacaaca 25920 agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg ggaatctgga 25980 gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca gctgtactca 26040 actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta caataaaatt 26100 gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acggttcatc cggagttgtt 26160 aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt gcctttgtaa 26220 gcacaagctg atgagtacga acttatgtac tcattcgttt cggaagagmc aggtacgtta 26280 atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt tacactagcc 26340 atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt gagtcttgta 26400 aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag agttcctgat 26460 cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact ttaattttag 26520 ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc cttgaacaat 26580 ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa tttgcctatg 26640 ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg ttatggccag 26700 taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc accggtggaa 26760 ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc attgcttctt 26820 tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc 26880 tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa 26940 tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta ggacgctgtg 27000 acatcaagga cctgcctaaa gaaatcactg ttgctacatc acgaatgctt tcttattaca 27060 aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac agtcgctaca 27120 ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat attgctttgc 27180 ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac tatagcagag 27240 atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga ttacatcata 27300 aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc tcaattagat 27360 gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt cttggcactg 27420 ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg tacaacagta 27480 cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt tcatcctcta 27540 gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc ttgtcctgac 27600 ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact gttcatcaga 27660 caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc aatagtgttt 27720 ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca ttaattgact 27780 tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt attatctttt 27840 ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg aacatgaaat 27900 ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa tgtagtttac 27960 agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt cacttctatt 28020 ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa ttgtgcgtgg 28080 atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat acagtttcct 28140 gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta gtgcgttgtt 28200 cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat ttcatctaaa 28260 cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac 28320 gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca gtggggcgcg 28380 atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt tcaccgctct 28440 cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc caattaacac 28500 caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac gaattcgtgg 28560 tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc taggaactgg 28620 gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg ttgcaactga 28680 gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta acaatgctgc 28740 aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg cagaagggag 28800 cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa 28860 ttcaactcca ggcagcagta aacgaacttc tcctgctaga atggctggca atggcggtga 28920 tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca aaatgtctgg 28980 taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa 29040 gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag 29100 acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca gacaaggaac 29160 tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag cgttcttcgg 29220 aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct acacaggtgc 29280 catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca 29340 tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc 29400 tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc 29460 tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc 29520 aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acgttttcgc 29580 ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc 29640 acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg tgtaacatta 29700 gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac gatcgagtgt 29760 acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg taaaattaat 29820 tttagtagtg ctatccccat gtgattttaa tagcttctta ggagnatgac annnnnnnnn 29880 nnnnn 29885 <210> 16 <211> 4405 <212> PRT <213> SARS-CoV2 <400> 16 Met Glu Ser Leu Val Pro Gly Phe Asn Glu Lys Thr His Val Gln Leu 1 5 10 15 Ser Leu Pro Val Leu Gln Val Arg Asp Val Leu Val Arg Gly Phe Gly 20 25 30 Asp Ser Val Glu Glu Val Leu Ser Glu Ala Arg Gln His Leu Lys Asp 35 40 45 Gly Thr Cys Gly Leu Val Glu Val Glu Lys Gly Val Leu Pro Gln Leu 50 55 60 Glu Gln Pro Tyr Val Phe Ile Lys Arg Ser Asp Ala Arg Thr Ala Pro 65 70 75 80 His Gly His Val Met Val Glu Leu Val Ala Glu Leu Glu Gly Ile Gln 85 90 95 Tyr Gly Arg Ser Gly Glu Thr Leu Gly Val Leu Val Pro His Val Gly 100 105 110 Glu Ile Pro Val Ala Tyr Arg Lys Val Leu Leu Arg Lys Asn Gly Asn 115 120 125 Lys Gly Ala Gly Gly His Ser Tyr Gly Ala Asp Leu Lys Ser Phe Asp 130 135 140 Leu Gly Asp Glu Leu Gly Thr Asp Pro Tyr Glu Asp Phe Gln Glu Asn 145 150 155 160 Trp Asn Thr Lys His Ser Ser Gly Val Thr Arg Glu Leu Met Arg Glu 165 170 175 Leu Asn Gly Gly Ala Tyr Thr Arg Tyr Val Asp Asn Asn Phe Cys Gly 180 185 190 Pro Asp Gly Tyr Pro Leu Glu Cys Ile Lys Asp Leu Leu Ala Arg Ala 195 200 205 Gly Lys Ala Ser Cys Thr Leu Ser Glu Gln Leu Asp Phe Ile Asp Thr 210 215 220 Lys Arg Gly Val Tyr Cys Cys Arg Glu His Glu His Glu Ile Ala Trp 225 230 235 240 Tyr Thr Glu Arg Ser Glu Lys Ser Tyr Glu Leu Gln Thr Pro Phe Glu 245 250 255 Ile Lys Leu Ala Lys Lys Phe Asp Thr Phe Asn Gly Glu Cys Pro Asn 260 265 270 Phe Val Phe Pro Leu Asn Ser Ile Ile Lys Thr Ile Gln Pro Arg Val 275 280 285 Glu Lys Lys Lys Leu Asp Gly Phe Met Gly Arg Ile Arg Ser Val Tyr 290 295 300 Pro Val Ala Ser Pro Asn Glu Cys Asn Gln Met Cys Leu Ser Thr Leu 305 310 315 320 Met Lys Cys Asp His Cys Gly Glu Thr Ser Trp Gln Thr Gly Asp Phe 325 330 335 Val Lys Ala Thr Cys Glu Phe Cys Gly Thr Glu Asn Leu Thr Lys Glu 340 345 350 Gly Ala Thr Thr Cys Gly Tyr Leu Pro Gln Asn Ala Val Val Lys Ile 355 360 365 Tyr Cys Pro Ala Cys His Asn Ser Glu Val Gly Pro Glu His Ser Leu 370 375 380 Ala Glu Tyr His Asn Glu Ser Gly Leu Lys Thr Ile Leu Arg Lys Gly 385 390 395 400 Gly Arg Thr Ile Ala Phe Gly Gly Cys Val Phe Ser Tyr Val Gly Cys 405 410 415 His Asn Lys Cys Ala Tyr Trp Val Pro Arg Ala Ser Ala Asn Ile Gly 420 425 430 Cys Asn His Thr Gly Val Val Gly Glu Gly Ser Glu Gly Leu Asn Asp 435 440 445 Asn Leu Leu Glu Ile Leu Gln Lys Glu Lys Val Asn Ile Asn Ile Val 450 455 460 Gly Asp Phe Lys Leu Asn Glu Glu Ile Ala Ile Ile Leu Ala Ser Phe 465 470 475 480 Ser Ala Ser Thr Ser Ala Phe Val Glu Thr Val Lys Gly Leu Asp Tyr 485 490 495 Lys Ala Phe Lys Gln Ile Val Glu Ser Cys Gly Asn Phe Lys Val Thr 500 505 510 Lys Gly Lys Ala Lys Lys Gly Ala Trp Asn Ile Gly Glu Gln Lys Ser 515 520 525 Ile Leu Ser Pro Leu Tyr Ala Phe Ala Ser Glu Ala Ala Arg Val Val 530 535 540 Arg Ser Ile Phe Ser Arg Thr Leu Glu Thr Ala Gln Asn Ser Val Arg 545 550 555 560 Val Leu Gln Lys Ala Ala Ile Thr Ile Leu Asp Gly Ile Ser Gln Tyr 565 570 575 Ser Leu Arg Leu Ile Asp Ala Met Met Phe Thr Ser Asp Leu Ala Thr 580 585 590 Asn Asn Leu Val Val Met Ala Tyr Ile Thr Gly Gly Val Val Gln Leu 595 600 605 Thr Ser Gln Trp Leu Thr Asn Ile Phe Gly Thr Val Tyr Glu Lys Leu 610 615 620 Lys Pro Val Leu Asp Trp Leu Glu Glu Lys Phe Lys Glu Gly Val Glu 625 630 635 640 Phe Leu Arg Asp Gly Trp Glu Ile Val Lys Phe Ile Ser Thr Cys Ala 645 650 655 Cys Glu Ile Val Gly Gly Gln Ile Val Thr Cys Ala Lys Glu Ile Lys 660 665 670 Glu Ser Val Gln Thr Phe Phe Lys Leu Val Asn Lys Phe Leu Ala Leu 675 680 685 Cys Ala Asp Ser Ile Ile Ile Gly Gly Ala Lys Leu Lys Ala Leu Asn 690 695 700 Leu Gly Glu Thr Phe Val Thr His Ser Lys Gly Leu Tyr Arg Lys Cys 705 710 715 720 Val Lys Ser Arg Glu Glu Thr Gly Leu Leu Met Pro Leu Lys Ala Pro 725 730 735 Lys Glu Ile Ile Phe Leu Glu Gly Glu Thr Leu Pro Thr Glu Val Leu 740 745 750 Thr Glu Glu Val Val Leu Lys Thr Gly Asp Leu Gln Pro Leu Glu Gln 755 760 765 Pro Thr Ser Glu Ala Val Glu Ala Pro Leu Val Gly Thr Pro Val Cys 770 775 780 Ile Asn Gly Leu Met Leu Leu Glu Ile Lys Asp Thr Glu Lys Tyr Cys 785 790 795 800 Ala Leu Ala Pro Asn Met Met Val Thr Asn Asn Thr Phe Thr Leu Lys 805 810 815 Gly Gly Ala Pro Thr Lys Val Thr Phe Gly Asp Asp Thr Val Ile Glu 820 825 830 Val Gln Gly Tyr Lys Ser Val Asn Ile Thr Phe Glu Leu Asp Glu Arg 835 840 845 Ile Asp Lys Val Leu Asn Glu Lys Cys Ser Ala Tyr Thr Val Glu Leu 850 855 860 Gly Thr Glu Val Asn Glu Phe Ala Cys Val Val Ala Asp Ala Val Ile 865 870 875 880 Lys Thr Leu Gln Pro Val Ser Glu Leu Leu Thr Pro Leu Gly Ile Asp 885 890 895 Leu Asp Glu Trp Ser Met Ala Thr Tyr Tyr Leu Phe Asp Glu Ser Gly 900 905 910 Glu Phe Lys Leu Ala Ser His Met Tyr Cys Ser Phe Tyr Pro Pro Asp 915 920 925 Glu Asp Glu Glu Glu Gly Asp Cys Glu Glu Glu Glu Phe Glu Pro Ser 930 935 940 Thr Gln Tyr Glu Tyr Gly Thr Glu Asp Asp Tyr Gln Gly Lys Pro Leu 945 950 955 960 Glu Phe Gly Ala Thr Ser Ala Ala Leu Gln Pro Glu Glu Glu Gln Glu 965 970 975 Glu Asp Trp Leu Asp Asp Asp Ser Gln Gln Thr Val Gly Gln Gln Asp 980 985 990 Gly Ser Glu Asp Asn Gln Thr Thr Thr Ile Gln Thr Ile Val Glu Val 995 1000 1005 Gln Pro Gln Leu Glu Met Glu Leu Thr Pro Val Val Gln Thr Ile Glu 1010 1015 1020 Val Asn Ser Phe Ser Gly Tyr Leu Lys Leu Thr Asp Asn Val Tyr Ile 1025 1030 1035 1040 Lys Asn Ala Asp Ile Val Glu Glu Ala Lys Lys Val Lys Pro Thr Val 1045 1050 1055 Val Val Asn Ala Ala Asn Val Tyr Leu Lys His Gly Gly Gly Val Ala 1060 1065 1070 Gly Ala Leu Asn Lys Ala Thr Asn Asn Ala Met Gln Val Glu Ser Asp 1075 1080 1085 Asp Tyr Ile Ala Thr Asn Gly Pro Leu Lys Val Gly Gly Ser Cys Val 1090 1095 1100 Leu Ser Gly His Asn Leu Ala Lys His Cys Leu His Val Val Gly Pro 1105 1110 1115 1120 Asn Val Asn Lys Gly Glu Asp Ile Gln Leu Leu Lys Ser Ala Tyr Glu 1125 1130 1135 Asn Phe Asn Gln His Glu Val Leu Leu Ala Pro Leu Leu Ser Ala Gly 1140 1145 1150 Ile Phe Gly Ala Asp Pro Ile His Ser Leu Arg Val Cys Val Asp Thr 1155 1160 1165 Val Arg Thr Asn Val Tyr Leu Ala Val Phe Asp Lys Asn Leu Tyr Asp 1170 1175 1180 Lys Leu Val Ser Ser Phe Leu Glu Met Lys Ser Glu Lys Gln Val Glu 1185 1190 1195 1200 Gln Lys Ile Ala Glu Ile Pro Lys Glu Glu Val Lys Pro Phe Ile Thr 1205 1210 1215 Glu Ser Lys Pro Ser Val Glu Gln Arg Lys Gln Asp Asp Lys Lys Ile 1220 1225 1230 Lys Ala Cys Val Glu Glu Val Thr Thr Thr Leu Glu Glu Thr Lys Phe 1235 1240 1245 Leu Thr Glu Asn Leu Leu Leu Tyr Ile Asp Ile Asn Gly Asn Leu His 1250 1255 1260 Pro Asp Ser Ala Thr Leu Val Ser Asp Ile Asp Ile Thr Phe Leu Lys 1265 1270 1275 1280 Lys Asp Ala Pro Tyr Ile Val Gly Asp Val Val Gln Glu Gly Val Leu 1285 1290 1295 Thr Ala Val Val Ile Pro Thr Lys Lys Ala Gly Gly Thr Thr Glu Met 1300 1305 1310 Leu Ala Lys Ala Leu Arg Lys Val Pro Thr Asp Asn Tyr Ile Thr Thr 1315 1320 1325 Tyr Pro Gly Gln Gly Leu Asn Gly Tyr Thr Val Glu Glu Ala Lys Thr 1330 1335 1340 Val Leu Lys Lys Cys Lys Ser Ala Phe Tyr Ile Leu Pro Ser Ile Ile 1345 1350 1355 1360 Ser Asn Glu Lys Gln Glu Ile Leu Gly Thr Val Ser Trp Asn Leu Arg 1365 1370 1375 Glu Met Leu Ala His Ala Glu Glu Thr Arg Lys Leu Met Pro Val Cys 1380 1385 1390 Val Glu Thr Lys Ala Ile Val Ser Thr Ile Gln Arg Lys Tyr Lys Gly 1395 1400 1405 Ile Lys Ile Gln Glu Gly Val Val Asp Tyr Gly Ala Arg Phe Tyr Phe 1410 1415 1420 Tyr Thr Ser Lys Thr Thr Val Ala Ser Leu Ile Asn Thr Leu Asn Asp 1425 1430 1435 1440 Leu Asn Glu Thr Leu Val Thr Met Pro Leu Gly Tyr Val Thr His Gly 1445 1450 1455 Leu Asn Leu Glu Glu Ala Ala Arg Tyr Met Arg Ser Leu Lys Val Pro 1460 1465 1470 Ala Thr Val Ser Val Ser Ser Pro Asp Ala Val Thr Ala Tyr Asn Gly 1475 1480 1485 Tyr Leu Thr Ser Ser Ser Lys Thr Pro Glu Glu His Phe Ile Glu Thr 1490 1495 1500 Ile Ser Leu Ala Gly Ser Tyr Lys Asp Trp Ser Tyr Ser Gly Gln Ser 1505 1510 1515 1520 Thr Gln Leu Gly Ile Glu Phe Leu Lys Arg Gly Asp Lys Ser Val Tyr 1525 1530 1535 Tyr Thr Ser Asn Pro Thr Thr Phe His Leu Asp Gly Glu Val Ile Thr 1540 1545 1550 Phe Asp Asn Leu Lys Thr Leu Leu Ser Leu Arg Glu Val Arg Thr Ile 1555 1560 1565 Lys Val Phe Thr Thr Val Asp Asn Ile Asn Leu His Thr Gln Val Val 1570 1575 1580 Asp Met Ser Met Thr Tyr Gly Gln Gln Phe Gly Pro Thr Tyr Leu Asp 1585 1590 1595 1600 Gly Ala Asp Val Thr Lys Ile Lys Pro His Asn Ser His Glu Gly Lys 1605 1610 1615 Thr Phe Tyr Val Leu Pro Asn Asp Asp Thr Leu Arg Val Glu Ala Phe 1620 1625 1630 Glu Tyr Tyr His Thr Thr Asp Pro Ser Phe Leu Gly Arg Tyr Met Ser 1635 1640 1645 Ala Leu Asn His Thr Lys Lys Trp Lys Tyr Pro Gln Val Asn Gly Leu 1650 1655 1660 Thr Ser Ile Lys Trp Ala Asp Asn Asn Cys Tyr Leu Ala Thr Ala Leu 1665 1670 1675 1680 Leu Thr Leu Gln Gln Ile Glu Leu Lys Phe Asn Pro Pro Ala Leu Gln 1685 1690 1695 Asp Ala Tyr Tyr Arg Ala Arg Ala Gly Glu Ala Ala Asn Phe Cys Ala 1700 1705 1710 Leu Ile Leu Ala Tyr Cys Asn Lys Thr Val Gly Glu Leu Gly Asp Val 1715 1720 1725 Arg Glu Thr Met Ser Tyr Leu Phe Gln His Ala Asn Leu Asp Ser Cys 1730 1735 1740 Lys Arg Val Leu Asn Val Val Cys Lys Thr Cys Gly Gln Gln Gln Thr 1745 1750 1755 1760 Thr Leu Lys Gly Val Glu Ala Val Met Tyr Met Gly Thr Leu Ser Tyr 1765 1770 1775 Glu Gln Phe Lys Lys Gly Val Gln Ile Pro Cys Thr Cys Gly Lys Gln 1780 1785 1790 Ala Thr Lys Tyr Leu Val Gln Gln Glu Ser Pro Phe Val Met Met Ser 1795 1800 1805 Ala Pro Pro Ala Gln Tyr Glu Leu Lys His Gly Thr Phe Thr Cys Ala 1810 1815 1820 Ser Glu Tyr Thr Gly Asn Tyr Gln Cys Gly His Tyr Lys His Ile Thr 1825 1830 1835 1840 Ser Lys Glu Thr Leu Tyr Cys Ile Asp Gly Ala Leu Leu Thr Lys Ser 1845 1850 1855 Ser Glu Tyr Lys Gly Pro Ile Thr Asp Val Phe Tyr Lys Glu Asn Ser 1860 1865 1870 Tyr Thr Thr Thr Ile Lys Pro Val Thr Tyr Lys Leu Asp Gly Val Val 1875 1880 1885 Cys Thr Glu Ile Asp Pro Lys Leu Asp Asn Tyr Tyr Lys Lys Asp Asn 1890 1895 1900 Ser Tyr Phe Thr Glu Gln Pro Ile Asp Leu Val Pro Asn Gln Pro Tyr 1905 1910 1915 1920 Pro Asn Ala Ser Phe Asp Asn Phe Lys Phe Val Cys Asp Asn Ile Lys 1925 1930 1935 Phe Ala Asp Asp Leu Asn Gln Leu Thr Gly Tyr Lys Lys Pro Ala Ser 1940 1945 1950 Arg Glu Leu Lys Val Thr Phe Phe Pro Asp Leu Asn Gly Asp Val Val 1955 1960 1965 Ala Ile Asp Tyr Lys His Tyr Thr Pro Ser Phe Lys Lys Gly Ala Lys 1970 1975 1980 Leu Leu His Lys Pro Ile Val Trp His Val Asn Asn Ala Thr Asn Lys 1985 1990 1995 2000 Ala Thr Tyr Lys Pro Asn Thr Trp Cys Ile Arg Cys Leu Trp Ser Thr 2005 2010 2015 Lys Pro Val Glu Thr Ser Asn Ser Phe Asp Val Leu Lys Ser Glu Asp 2020 2025 2030 Ala Gln Gly Met Asp Asn Leu Ala Cys Glu Asp Leu Lys Pro Val Ser 2035 2040 2045 Glu Glu Val Val Glu Asn Pro Thr Ile Gln Lys Asp Val Leu Glu Cys 2050 2055 2060 Asn Val Lys Thr Thr Glu Val Val Gly Asp Ile Ile Leu Lys Pro Ala 2065 2070 2075 2080 Asn Asn Ser Leu Lys Ile Thr Glu Glu Val Gly His Thr Asp Leu Met 2085 2090 2095 Ala Ala Tyr Val Asp Asn Ser Ser Leu Thr Ile Lys Lys Pro Asn Glu 2100 2105 2110 Leu Ser Arg Val Leu Gly Leu Lys Thr Leu Ala Thr His Gly Leu Ala 2115 2120 2125 Ala Val Asn Ser Val Pro Trp Asp Thr Ile Ala Asn Tyr Ala Lys Pro 2130 2135 2140 Phe Leu Asn Lys Val Val Ser Thr Thr Thr Asn Ile Val Thr Arg Cys 2145 2150 2155 2160 Leu Asn Arg Val Cys Thr Asn Tyr Met Pro Tyr Phe Phe Thr Leu Leu 2165 2170 2175 Leu Gln Leu Cys Thr Phe Thr Arg Ser Thr Asn Ser Arg Ile Lys Ala 2180 2185 2190 Ser Met Pro Thr Thr Ile Ala Lys Asn Thr Val Lys Ser Val Gly Lys 2195 2200 2205 Phe Cys Leu Glu Ala Ser Phe Asn Tyr Leu Lys Ser Pro Asn Phe Ser 2210 2215 2220 Lys Leu Ile Asn Ile Ile Ile Trp Phe Leu Leu Leu Ser Val Cys Leu 2225 2230 2235 2240 Gly Ser Leu Ile Tyr Ser Thr Ala Ala Leu Gly Val Leu Met Ser Asn 2245 2250 2255 Leu Gly Met Pro Ser Tyr Cys Thr Gly Tyr Arg Glu Gly Tyr Leu Asn 2260 2265 2270 Ser Thr Asn Val Thr Ile Ala Thr Tyr Cys Thr Gly Ser Ile Pro Cys 2275 2280 2285 Ser Val Cys Leu Ser Gly Leu Asp Ser Leu Asp Thr Tyr Pro Ser Leu 2290 2295 2300 Glu Thr Ile Gln Ile Thr Ile Ser Ser Phe Lys Trp Asp Leu Thr Ala 2305 2310 2315 2320 Phe Gly Leu Val Ala Glu Trp Phe Leu Ala Tyr Ile Leu Phe Thr Arg 2325 2330 2335 Phe Phe Tyr Val Leu Gly Leu Ala Ala Ile Met Gln Leu Phe Phe Ser 2340 2345 2350 Tyr Phe Ala Val His Phe Ile Ser Asn Ser Trp Leu Met Trp Leu Ile 2355 2360 2365 Ile Asn Leu Val Gln Met Ala Pro Ile Ser Ala Met Val Arg Met Tyr 2370 2375 2380 Ile Phe Phe Ala Ser Phe Tyr Tyr Val Trp Lys Ser Tyr Val His Val 2385 2390 2395 2400 Val Asp Gly Cys Asn Ser Ser Thr Cys Met Met Cys Tyr Lys Arg Asn 2405 2410 2415 Arg Ala Thr Arg Val Glu Cys Thr Thr Ile Val Asn Gly Val Arg Arg 2420 2425 2430 Ser Phe Tyr Val Tyr Ala Asn Gly Gly Lys Gly Phe Cys Lys Leu His 2435 2440 2445 Asn Trp Asn Cys Val Asn Cys Asp Thr Phe Cys Ala Gly Ser Thr Phe 2450 2455 2460 Ile Ser Asp Glu Val Ala Arg Asp Leu Ser Leu Gln Phe Lys Arg Pro 2465 2470 2475 2480 Ile Asn Pro Thr Asp Gln Ser Ser Tyr Ile Val Asp Ser Val Thr Val 2485 2490 2495 Lys Asn Gly Ser Ile His Leu Tyr Phe Asp Lys Ala Gly Gln Lys Thr 2500 2505 2510 Tyr Glu Arg His Ser Leu Ser His Phe Val Asn Leu Asp Asn Leu Arg 2515 2520 2525 Ala Asn Asn Thr Lys Gly Ser Leu Pro Ile Asn Val Ile Val Phe Asp 2530 2535 2540 Gly Lys Ser Lys Cys Glu Glu Ser Ser Ala Lys Ser Ala Ser Val Tyr 2545 2550 2555 2560 Tyr Ser Gln Leu Met Cys Gln Pro Ile Leu Leu Leu Asp Gln Ala Leu 2565 2570 2575 Val Ser Asp Val Gly Asp Ser Ala Glu Val Ala Val Lys Met Phe Asp 2580 2585 2590 Ala Tyr Val Asn Thr Phe Ser Ser Thr Phe Asn Val Pro Met Glu Lys 2595 2600 2605 Leu Lys Thr Leu Val Ala Thr Ala Glu Ala Glu Leu Ala Lys Asn Val 2610 2615 2620 Ser Leu Asp Asn Val Leu Ser Thr Phe Ile Ser Ala Ala Arg Gln Gly 2625 2630 2635 2640 Phe Val Asp Ser Asp Val Glu Thr Lys Asp Val Val Glu Cys Leu Lys 2645 2650 2655 Leu Ser His Gln Ser Asp Ile Glu Val Thr Gly Asp Ser Cys Asn Asn 2660 2665 2670 Tyr Met Leu Thr Tyr Asn Lys Val Glu Asn Met Thr Pro Arg Asp Leu 2675 2680 2685 Gly Ala Cys Ile Asp Cys Ser Ala Arg His Ile Asn Ala Gln Val Ala 2690 2695 2700 Lys Ser His Asn Ile Ala Leu Ile Trp Asn Val Lys Asp Phe Met Ser 2705 2710 2715 2720 Leu Ser Glu Gln Leu Arg Lys Gln Ile Arg Ser Ala Ala Lys Lys Asn 2725 2730 2735 Asn Leu Pro Phe Lys Leu Thr Cys Ala Thr Thr Arg Gln Val Val Asn 2740 2745 2750 Val Val Thr Thr Lys Ile Ala Leu Lys Gly Gly Lys Ile Val Asn Asn 2755 2760 2765 Trp Leu Lys Gln Leu Ile Lys Val Thr Leu Val Phe Leu Phe Val Ala 2770 2775 2780 Ala Ile Phe Tyr Leu Ile Thr Pro Val His Val Met Ser Lys His Thr 2785 2790 2795 2800 Asp Phe Ser Ser Glu Ile Ile Gly Tyr Lys Ala Ile Asp Gly Gly Val 2805 2810 2815 Thr Arg Asp Ile Ala Ser Thr Asp Thr Cys Phe Ala Asn Lys His Ala 2820 2825 2830 Asp Phe Asp Thr Trp Phe Ser Gln Arg Gly Gly Ser Tyr Thr Asn Asp 2835 2840 2845 Lys Ala Cys Pro Leu Ile Ala Ala Val Ile Thr Arg Glu Val Gly Phe 2850 2855 2860 Val Val Pro Gly Leu Pro Gly Thr Ile Leu Arg Thr Thr Asn Gly Asp 2865 2870 2875 2880 Phe Leu His Phe Leu Pro Arg Val Phe Ser Ala Val Gly Asn Ile Cys 2885 2890 2895 Tyr Thr Pro Ser Lys Leu Ile Glu Tyr Thr Asp Phe Ala Thr Ser Ala 2900 2905 2910 Cys Val Leu Ala Ala Glu Cys Thr Ile Phe Lys Asp Ala Ser Gly Lys 2915 2920 2925 Pro Val Pro Tyr Cys Tyr Asp Thr Asn Val Leu Glu Gly Ser Val Ala 2930 2935 2940 Tyr Glu Ser Leu Arg Pro Asp Thr Arg Tyr Val Leu Met Asp Gly Ser 2945 2950 2955 2960 Ile Ile Gln Phe Pro Asn Thr Tyr Leu Glu Gly Ser Val Arg Val Val 2965 2970 2975 Thr Thr Phe Asp Ser Glu Tyr Cys Arg His Gly Thr Cys Glu Arg Ser 2980 2985 2990 Glu Ala Gly Val Cys Val Ser Thr Ser Gly Arg Trp Val Leu Asn Asn 2995 3000 3005 Asp Tyr Tyr Arg Ser Leu Pro Gly Val Phe Cys Gly Val Asp Ala Val 3010 3015 3020 Asn Leu Leu Thr Asn Met Phe Thr Pro Leu Ile Gln Pro Ile Gly Ala 3025 3030 3035 3040 Leu Asp Ile Ser Ala Ser Ile Val Ala Gly Gly Ile Val Ala Ile Val 3045 3050 3055 Val Thr Cys Leu Ala Tyr Tyr Phe Met Arg Phe Arg Arg Ala Phe Gly 3060 3065 3070 Glu Tyr Ser His Val Val Ala Phe Asn Thr Leu Leu Phe Leu Met Ser 3075 3080 3085 Phe Thr Val Leu Cys Leu Thr Pro Val Tyr Ser Phe Leu Pro Gly Val 3090 3095 3100 Tyr Ser Val Ile Tyr Leu Tyr Leu Thr Phe Tyr Leu Thr Asn Asp Val 3105 3110 3115 3120 Ser Phe Leu Ala His Ile Gln Trp Met Val Met Phe Thr Pro Leu Val 3125 3130 3135 Pro Phe Trp Ile Thr Ile Ala Tyr Ile Ile Cys Ile Ser Thr Lys His 3140 3145 3150 Phe Tyr Trp Phe Phe Ser Asn Tyr Leu Lys Arg Arg Val Val Phe Asn 3155 3160 3165 Gly Val Ser Phe Ser Thr Phe Glu Glu Ala Ala Leu Cys Thr Phe Leu 3170 3175 3180 Leu Asn Lys Glu Met Tyr Leu Lys Leu Arg Ser Asp Val Leu Leu Pro 3185 3190 3195 3200 Leu Thr Gln Tyr Asn Arg Tyr Leu Ala Leu Tyr Asn Lys Tyr Lys Tyr 3205 3210 3215 Phe Ser Gly Ala Met Asp Thr Thr Ser Tyr Arg Glu Ala Ala Cys Cys 3220 3225 3230 His Leu Ala Lys Ala Leu Asn Asp Phe Ser Asn Ser Gly Ser Asp Val 3235 3240 3245 Leu Tyr Gln Pro Pro Gln Thr Ser Ile Thr Ser Ala Val Leu Gln Ser 3250 3255 3260 Gly Phe Arg Lys Met Ala Phe Pro Ser Gly Lys Val Glu Gly Cys Met 3265 3270 3275 3280 Val Gln Val Thr Cys Gly Thr Thr Thr Leu Asn Gly Leu Trp Leu Asp 3285 3290 3295 Asp Val Val Tyr Cys Pro Arg His Val Ile Cys Thr Ser Glu Asp Met 3300 3305 3310 Leu Asn Pro Asn Tyr Glu Asp Leu Leu Ile Arg Lys Ser Asn His Asn 3315 3320 3325 Phe Leu Val Gln Ala Gly Asn Val Gln Leu Arg Val Ile Gly His Ser 3330 3335 3340 Met Gln Asn Cys Val Leu Lys Leu Lys Val Asp Thr Ala Asn Pro Lys 3345 3350 3355 3360 Thr Pro Lys Tyr Lys Phe Val Arg Ile Gln Pro Gly Gln Thr Phe Ser 3365 3370 3375 Val Leu Ala Cys Tyr Asn Gly Ser Pro Ser Gly Val Tyr Gln Cys Ala 3380 3385 3390 Met Arg Pro Asn Phe Thr Ile Lys Gly Ser Phe Leu Asn Gly Ser Cys 3395 3400 3405 Gly Ser Val Gly Phe Asn Ile Asp Tyr Asp Cys Val Ser Phe Cys Tyr 3410 3415 3420 Met His His Met Glu Leu Pro Thr Gly Val His Ala Gly Thr Asp Leu 3425 3430 3435 3440 Glu Gly Asn Phe Tyr Gly Pro Phe Val Asp Arg Gln Thr Ala Gln Ala 3445 3450 3455 Ala Gly Thr Asp Thr Thr Ile Thr Val Asn Val Leu Ala Trp Leu Tyr 3460 3465 3470 Ala Ala Val Ile Asn Gly Asp Arg Trp Phe Leu Asn Arg Phe Thr Thr 3475 3480 3485 Thr Leu Asn Asp Phe Asn Leu Val Ala Met Lys Tyr Asn Tyr Glu Pro 3490 3495 3500 Leu Thr Gln Asp His Val Asp Ile Leu Gly Pro Leu Ser Ala Gln Thr 3505 3510 3515 3520 Gly Ile Ala Val Leu Asp Met Cys Ala Ser Leu Lys Glu Leu Leu Gln 3525 3530 3535 Asn Gly Met Asn Gly Arg Thr Ile Leu Gly Ser Ala Leu Leu Glu Asp 3540 3545 3550 Glu Phe Thr Pro Phe Asp Val Val Arg Gln Cys Ser Gly Val Thr Phe 3555 3560 3565 Gln Ser Ala Val Lys Arg Thr Ile Lys Gly Thr His His Trp Leu Leu 3570 3575 3580 Leu Thr Ile Leu Thr Ser Leu Leu Val Leu Val Gln Ser Thr Gln Trp 3585 3590 3595 3600 Ser Leu Phe Phe Phe Leu Tyr Glu Asn Ala Phe Leu Pro Phe Ala Met 3605 3610 3615 Gly Ile Ile Ala Met Ser Ala Phe Ala Met Met Phe Val Lys His Lys 3620 3625 3630 His Ala Phe Leu Cys Leu Phe Leu Leu Pro Ser Leu Ala Thr Val Ala 3635 3640 3645 Tyr Phe Asn Met Val Tyr Met Pro Ala Ser Trp Val Met Arg Ile Met 3650 3655 3660 Thr Trp Leu Asp Met Val Asp Thr Ser Leu Ser Gly Phe Lys Leu Lys 3665 3670 3675 3680 Asp Cys Val Met Tyr Ala Ser Ala Val Val Leu Leu Ile Leu Met Thr 3685 3690 3695 Ala Arg Thr Val Tyr Asp Asp Gly Ala Arg Arg Val Trp Thr Leu Met 3700 3705 3710 Asn Val Leu Thr Leu Val Tyr Lys Val Tyr Tyr Gly Asn Ala Leu Asp 3715 3720 3725 Gln Ala Ile Ser Met Trp Ala Leu Ile Ile Ser Val Thr Ser Asn Tyr 3730 3735 3740 Ser Gly Val Val Thr Thr Val Met Phe Leu Ala Arg Gly Ile Val Phe 3745 3750 3755 3760 Met Cys Val Glu Tyr Cys Pro Ile Phe Phe Ile Thr Gly Asn Thr Leu 3765 3770 3775 Gln Cys Ile Met Leu Val Tyr Cys Phe Leu Gly Tyr Phe Cys Thr Cys 3780 3785 3790 Tyr Phe Gly Leu Phe Cys Leu Leu Asn Arg Tyr Phe Arg Leu Thr Leu 3795 3800 3805 Gly Val Tyr Asp Tyr Leu Val Ser Thr Gln Glu Phe Arg Tyr Met Asn 3810 3815 3820 Ser Gln Gly Leu Leu Pro Pro Lys Asn Ser Ile Asp Ala Phe Lys Leu 3825 3830 3835 3840 Asn Ile Lys Leu Leu Gly Val Gly Gly Lys Pro Cys Ile Lys Val Ala 3845 3850 3855 Thr Val Gln Ser Lys Met Ser Asp Val Lys Cys Thr Ser Val Val Leu 3860 3865 3870 Leu Ser Val Leu Gln Gln Leu Arg Val Glu Ser Ser Ser Lys Leu Trp 3875 3880 3885 Ala Gln Cys Val Gln Leu His Asn Asp Ile Leu Leu Ala Lys Asp Thr 3890 3895 3900 Thr Glu Ala Phe Glu Lys Met Val Ser Leu Leu Ser Val Leu Leu Ser 3905 3910 3915 3920 Met Gln Gly Ala Val Asp Ile Asn Lys Leu Cys Glu Glu Met Leu Asp 3925 3930 3935 Asn Arg Ala Thr Leu Gln Ala Ile Ala Ser Glu Phe Ser Ser Leu Pro 3940 3945 3950 Ser Tyr Ala Ala Phe Ala Thr Ala Gln Glu Ala Tyr Glu Gln Ala Val 3955 3960 3965 Ala Asn Gly Asp Ser Glu Val Val Leu Lys Lys Leu Lys Lys Ser Leu 3970 3975 3980 Asn Val Ala Lys Ser Glu Phe Asp Arg Asp Ala Ala Met Gln Arg Lys 3985 3990 3995 4000 Leu Glu Lys Met Ala Asp Gln Ala Met Thr Gln Met Tyr Lys Gln Ala 4005 4010 4015 Arg Ser Glu Asp Lys Arg Ala Lys Val Thr Ser Ala Met Gln Thr Met 4020 4025 4030 Leu Phe Thr Met Leu Arg Lys Leu Asp Asn Asp Ala Leu Asn Asn Ile 4035 4040 4045 Ile Asn Asn Ala Arg Asp Gly Cys Val Pro Leu Asn Ile Ile Pro Leu 4050 4055 4060 Thr Thr Ala Ala Lys Leu Met Val Val Ile Pro Asp Tyr Asn Thr Tyr 4065 4070 4075 4080 Lys Asn Thr Cys Asp Gly Thr Thr Phe Thr Tyr Ala Ser Ala Leu Trp 4085 4090 4095 Glu Ile Gln Gln Val Val Asp Ala Asp Ser Lys Ile Val Gln Leu Ser 4100 4105 4110 Glu Ile Ser Met Asp Asn Ser Pro Asn Leu Ala Trp Pro Leu Ile Val 4115 4120 4125 Thr Ala Leu Arg Ala Asn Ser Ala Val Lys Leu Gln Asn Asn Glu Leu 4130 4135 4140 Ser Pro Val Ala Leu Arg Gln Met Ser Cys Ala Ala Gly Thr Thr Gln 4145 4150 4155 4160 Thr Ala Cys Thr Asp Asp Asn Ala Leu Ala Tyr Tyr Asn Thr Thr Lys 4165 4170 4175 Gly Gly Arg Phe Val Leu Ala Leu Leu Ser Asp Leu Gln Asp Leu Lys 4180 4185 4190 Trp Ala Arg Phe Pro Lys Ser Asp Gly Thr Gly Thr Ile Tyr Thr Glu 4195 4200 4205 Leu Glu Pro Pro Cys Arg Phe Val Thr Asp Thr Pro Lys Gly Pro Lys 4210 4215 4220 Val Lys Tyr Leu Tyr Phe Ile Lys Gly Leu Asn Asn Leu Asn Arg Gly 4225 4230 4235 4240 Met Val Leu Gly Ser Leu Ala Ala Thr Val Arg Leu Gln Ala Gly Asn 4245 4250 4255 Ala Thr Glu Val Pro Ala Asn Ser Thr Val Leu Ser Phe Cys Ala Phe 4260 4265 4270 Ala Val Asp Ala Ala Lys Ala Tyr Lys Asp Tyr Leu Ala Ser Gly Gly 4275 4280 4285 Gln Pro Ile Thr Asn Cys Val Lys Met Leu Cys Thr His Thr Gly Thr 4290 4295 4300 Gly Gln Ala Ile Thr Val Thr Pro Glu Ala Asn Met Asp Gln Glu Ser 4305 4310 4315 4320 Phe Gly Gly Ala Ser Cys Cys Leu Tyr Cys Arg Cys His Ile Asp His 4325 4330 4335 Pro Asn Pro Lys Gly Phe Cys Asp Leu Lys Gly Lys Tyr Val Gln Ile 4340 4345 4350 Pro Thr Thr Cys Ala Asn Asp Pro Val Gly Phe Thr Leu Lys Asn Thr 4355 4360 4365 Val Cys Thr Val Cys Gly Met Trp Lys Gly Tyr Gly Cys Ser Cys Asp 4370 4375 4380 Gln Leu Arg Glu Pro Met Leu Gln Ser Ala Asp Ala Gln Ser Phe Leu 4385 4390 4395 4400 Asn Gly Phe Ala Val 4405 <210> 17 <211> 1273 <212> PRT <213> SARS-CoV2 <220> <221> MISC_FEATURE <222> (320) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (709) <223> Xaa can be any naturally occurring amino acid <400> 17 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 225 230 235 240 Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 245 250 255 Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 260 265 270 Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 275 280 285 Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 290 295 300 Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Xaa 305 310 315 320 Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 325 330 335 Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 340 345 350 Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 355 360 365 Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 370 375 380 Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 385 390 395 400 Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 405 410 415 Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 420 425 430 Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 435 440 445 Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 450 455 460 Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 465 470 475 480 Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 485 490 495 Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 500 505 510 Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 515 520 525 Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 530 535 540 Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 545 550 555 560 Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 565 570 575 Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 580 585 590 Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 595 600 605 Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 610 615 620 His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 625 630 635 640 Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 645 650 655 Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 660 665 670 Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 675 680 685 Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 690 695 700 Val Ala Tyr Ser Xaa Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 705 710 715 720 Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 725 730 735 Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 740 745 750 Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 755 760 765 Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 770 775 780 Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 785 790 795 800 Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 805 810 815 Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 820 825 830 Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 835 840 845 Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 850 855 860 Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 865 870 875 880 Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 885 890 895 Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 900 905 910 Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 915 920 925 Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 930 935 940 Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 945 950 955 960 Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 965 970 975 Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 980 985 990 Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 995 1000 1005 Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Val Ser Ala Asn Leu 1010 1015 1020 Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 1025 1030 1035 1040 Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 1045 1050 1055 Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 1060 1065 1070 Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 1075 1080 1085 Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 1090 1095 1100 Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 1105 1110 1115 1120 Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 1125 1130 1135 Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 1140 1145 1150 Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 1155 1160 1165 Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 1170 1175 1180 Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 1185 1190 1195 1200 Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 1205 1210 1215 Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 1220 1225 1230 Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 1235 1240 1245 Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 1250 1255 1260 Leu Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 18 <211> 29897 <212> DNA <213> SARS-CoV2 <220> <221> misc_feature <222> (11288)..(11293) <223> n is a, c, g, or t <220> <221> misc_feature <222> (22278)..(22283) <223> n is a, c, g, or t <220> <221> misc_feature <222> (29836)..(29864) <223> n is a, c, g, or t <400> 18 attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 60 gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120 cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcttctatc 180 ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240 tgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 300 acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 360 agactccgtg gaggaggtct tatcagaggc acgtcaacat cttaaagatg gcacttgtgg 420 cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 480 acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 540 cgaaggcatt cagtacggtc gtagtggtga gacacttggt gtccttgtcc ctcatgtggg 600 cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 660 tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 720 tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 780 actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 840 ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 900 atgcactttg tccgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 960 tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1020 gacacctttt gaaattaaat tggcaaagaa atttgacatc ttcaatgggg aatgtccaaa 1080 ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1140 gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1200 caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1260 gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1320 aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1380 atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1440 cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc 1500 ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1560 ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 1620 aatactccaa aaagagaaag tcaacatcaa tattgttggt gactttaaac ttaatgaaga 1680 gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 1740 aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac 1800 aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1860 tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 1920 tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1980 aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 2040 taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg 2100 gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2160 agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 2220 ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcaa aggaaattaa 2280 ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 2340 tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca 2400 ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2460 tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 2520 aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2580 agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 2640 aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa ctaacaatac 2700 cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 2760 agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2820 acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 2880 ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc 2940 actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3000 tgagtttaaa ttggcttcac atatgtattg ttctttttac cctccagatg aggatgaaga 3060 agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3120 agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 3180 agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3240 cggcagtgag gacaatcaga caactactat tcaaacaatt gttgaggttc aacctcaatt 3300 agagatggaa cttacaccag ttgttcagac tattgaagtg aatagtttta gtggttattt 3360 aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 3420 aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc 3480 aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3540 tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 3600 acactgtctt catgttgtcg gcccaaatgt taacaaaggt gaagacattc aacttcttaa 3660 gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 3720 tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa 3780 tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3840 aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa 3900 gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3960 caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 4020 cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4080 tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttgttca 4140 agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat 4200 gttagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4260 gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc 4320 cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4380 ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg 4440 tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4500 agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 4560 gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4620 tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 4680 agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc 4740 ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4800 agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga 4860 taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4920 ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4980 aacagtagac aacattaacc tccacacgca agttgtggac atgtcaatga catatggaca 5040 acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 5100 acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5160 tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5220 cactaaaaat tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa 5280 caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5340 acctgctcta caagatgctt attacagagc aagggctggt gaagctgcta acttttgtgc 5400 acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5460 gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 5520 taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5580 cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 5640 agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5700 tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 5760 gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt 5820 acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5880 ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 5940 tgaccctaag ttggacaatt attataagaa agacaattct tatttcacag agcaaccaat 6000 tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 6060 tgataatatc aaatttgctg atgatttaaa ccagttaact ggttacaaga aacctgcttc 6120 aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6180 taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 6240 gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6300 tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 6360 cgcgcaggga atggataatc ttgcctgcga agatctaaaa ccagtctctg aagaagtagt 6420 ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 6480 aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6540 cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 6600 attatctaga gtattaggtt tgaaaaccct tgctactcat ggtttagctg ctgttaatag 6660 tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6720 aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 6780 ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc 6840 atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6900 ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttataatttg 6960 gtttttacta ttaagtgttt gcctaggttc tttaatmtac tcaaccgctg ctttaggtgt 7020 tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7080 ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7140 tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7200 atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat 7260 tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7320 ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 7380 acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7440 tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 7500 ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag 7560 gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7620 tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 7680 cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7740 tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7800 ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7860 taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7920 atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 7980 agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8040 tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 8100 agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac 8160 ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8220 tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 8280 ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8340 tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8400 atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc 8460 tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8520 tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 8580 gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8640 tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8700 tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc 8760 tgattttgac acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc 8820 attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 8880 gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8940 tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9000 ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata 9060 ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9120 acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 9180 tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9240 agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 9300 atctttacca ggagttttct gtggtgtaga tgctgtaaat ttatttacta atatgtttac 9360 accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9420 tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 9480 tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9540 ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9600 gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9660 cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9720 tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 9780 tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa 9840 gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9900 taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 9960 tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10020 accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10080 atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg 10140 tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10200 gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 10260 ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10320 tagggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 10380 acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc 10440 tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10500 ttttaacata gattatgact gtgtctcttt ttkttacatg caccatatgg aattaccaac 10560 tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10620 aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 10680 cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga 10740 ctttaacctt gtggctatga agtacaatta tgaacytcta acacaagacc atgttgacat 10800 actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 10860 agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10920 tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 10980 gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt 11040 agttttagtc cagagtactc aatggtcttt gttctttttt ttgtatgaaa atgccttttt 11100 accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 11160 gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11220 ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11280 tagtttgnnn nnnaagctaa aagactgtgt tatgtatgca tcagctgtag tgttactaat 11340 ccttatgaca gcaagaactg tgtatgatga tggtgctagg agagtgtgga cacttatgaa 11400 tgtcttgaca ctcgtttata aagtttatta tggtaatgct ttagatcaag ccatttccat 11460 gtgggctctt ataatctctg ttacttctaa ctactcaggt gtagttacaa ctgtcatgtt 11520 tttggccaga ggtattgttt ttatgtgtgt tgagtattgc cctattttct tcataactgg 11580 taatacactt cagtgtataa tgctagttta ttgtttctta ggctattttt gtacttgtta 11640 ctttggcctc ttttgtttac tcaaccgcta ctttagactg actcttggtg tttatgatta 11700 cttagtttct acacaggagt ttagatatat gaattcacag ggactaytcc cacccaagaa 11760 tagcatagat gccttcaaac tcaacattaa attgttgggt gttggtggca aaccttgtat 11820 caaagtagcc actgtacagt ctaaaatgtc agatgtaaag tgcacatcag tagtcttact 11880 ctcagttttg caacaactca gagtagaatc atcatctaaa ttgtgggctc aatgtgtcca 11940 gttacacaat gacattctct tagctaaaga tactactgaa gcctttgaaa aaatggtttc 12000 actactttct gttttgcttt ccatgcaggg tgctgtagac ataaacaagc tttgtgaaga 12060 aatgctggac aacagggcaa ccttacaagc tatagcctca gagtttagtt cccttccatc 12120 atatgcagct tttgctactg ctcaagaagc ttatgagcag gctgttgcta atggtgattc 12180 tgaagttgtt cttaaaaagt tgaagaagtc tttgaatgtg gctaaatctg aatttgaccg 12240 tgatgcagcc atgcaacgta agttggaaaa gatggctgat caagctatga cccaaatgta 12300 taaacaggct agatctgagg acaagagggc aaaagttact agtgctatgc agacaatgct 12360 tttcactatg cttagaaagt tggataatga tgcactcaac aacattatca acaatgcaag 12420 agatggttgt gttcccttga acataatacc tcttacaaca gcagccaaac taatggttgt 12480 cataccagac tataacacat ataaaaatac gtgtgatggt acaacattta cttatgcatc 12540 agcattgtgg gaaatccaac aggttgtaga tgcagatagt aaaattgttc aacttagtga 12600 aattagtatg gacaattcac ctaatttagc atggcctctt attgtaacag ctttaagggc 12660 caattctgct gtcaaattac agaataatga gcttagtcct gttgcactac gacagatgtc 12720 ttgtgctgcc ggtactacac aaactgcttg cactgatgac aatgcgttag cttactacaa 12780 cacaacaaag ggaggtaggt ttgtacttgc actgttatcc gatttacagg atttgaaatg 12840 ggctagattc cctaagagtg atggaactgg tactatctat acagaactgg aaccaccttg 12900 taggtttgtt acagacacac ctaaaggtcc taaagtgaag tatttatact ttattaaagg 12960 attaaacaac ctaaatagag gtatggtact tggtagttta gctgccacag tacgtctaca 13020 agctggtaat gcaacagaag tgcctgccaa ttcaactgta ttatctttct gtgcttttgc 13080 tgtagatgct gctaaagctt acaaagatta tctagctagt gggggacaac caatcactaa 13140 ttgtgttaag atgttgtgta cacacactgg tactggtcag gcaataacag ttacaccgga 13200 agccaatatg gatcaagaat cctttggtgg tgcatcgtgt tgtctgtact gccgttgcca 13260 catagatcat ccaaatccta aaggattttg tgacttaaaa ggtaagtatg tacaaatacc 13320 tacaacttgt gctaatgacc ctgtgggttt tacacttaaa aacacagtct gtaccgtctg 13380 cggtatgtgg aaaggttatg gctgtagttg tgatcaactc cgcgaaccca tgcttcagtc 13440 agctgatgca caatcgtttt taaacgggtt tgcggtgtaa gtgcagcccg tcttacaccg 13500 tgcggcacag gcactagtac tgatgtcgta tacagggctt ttgacatcta caatgataaa 13560 gtagctggtt ttgctaaatt cctaaaaact aattgttgtc gcttccaaga aaaggacgaa 13620 gatgacaatt taattgattc ttactttgta gttaagagac acactttctc taactaccaa 13680 catgaagaaa caatttataa tttacttaag gattgtccag ctgttgctaa acatgacttc 13740 tttaagttta gaatagacgg tgacatggta ccacatatat cacgtcaacg tcttactaaa 13800 tacacaatgg cagacctcgt ctatgcttta aggcattttg atgaaggtaa ttgtgacaca 13860 ttaaaagaaa tacttgtcac atacaattgt tgtgatgatg attatttcaa taaaaaggac 13920 tggtatgatt ttgtagaaaa cccagatata ttacgcgtat acgccaactt aggtgaacgt 13980 gtacgccaag ctttgttaaa aacagtacaa ttctgtgatg ccatgcgaaa tgctggtatt 14040 gttggtgtac tgacattaga taatcaagat ctcaatggta actggtatga tttcggtgat 14100 ttcatacaaa ccacgccagg tagtggagtt cctgttgtag attcttatta ttcattgtta 14160 atgcctatat taaccttgac cagggcttta actgcagagt cacatgttga cactgactta 14220 acaaagcctt acattaagtg ggatttgtta aaatatgact tcacggaaga gaggttaaaa 14280 ctctttgacc gttattttaa atattgggat cagacatacc acccaaattg tgttaactgt 14340 ttggatgaca gatgcattct gcattgtgca aactttaatg ttttattctc tacagtgttc 14400 ccacttacaa gttttggacc actagtgaga aaaatatttg ttgatggtgt tccatttgta 14460 gtttcaactg gataccactt cagagagcta ggtgttgtac ataatcagga tgtaaactta 14520 catagctcta gacttagttt taaggaatta cttgtgtatg ctgctgaccc tgctatgcac 14580 gctgcttctg gtaatctatt actagataaa cgcactacgt gcttttcagt agctgcactt 14640 actaacaatg ttgcttttca aactgtcaaa cccggtaatt ttaacaaaga cttctatgac 14700 tttgctgtgt ctaagggttt ctttaaggaa ggaagttctg ttgaattaaa acacttcttc 14760 tttgctcagg atggtaatgc tgctatcagc gattatgact actatcgtta taatctacca 14820 acaatgtgtg atatcagaca actactattt gtagttgaag ttgttgataa gtactttgat 14880 tgttacgatg gtggctgtat taatgctaac caagtcatcg tcaacaacct agacaaatca 14940 gctggttttc catttaataa atggggtaag gctagacttt attatgattc aatgagttat 15000 gaggatcaag atgcactttt cgcatataca aaacgtaatg tcatccctac tataactcaa 15060 atgaatctta agtatgccat tagtgcaaag aatagagctc gcaccgtagc tggtgtctct 15120 atctgtagta ctatgaccaa tagacagttt catcaaaaat tattgaaatc aatagccgcc 15180 actagaggag ctactgtagt aattggaaca agcaaattct atggtggttg gcacaacatg 15240 ttaaaaactg tttatagtga tgtagaaaac cctcacctta tgggttggga ttatcctaaa 15300 tgtgatagag ccatgcctaa catgcttaga attatggcct cacttgttct tgctcgcaaa 15360 catacaacgt gttgtagctt gtcacaccgt ttctatagat tagctaatga gtgtgctcaa 15420 gtattgagtg aaatggtcat gtgtggcggt tcactatatg ttaaaccagg tggaacctca 15480 tcaggagatg ccacaactgc ttatgctaat agtgttttta acatttgtca agctgtcacg 15540 gccaatgtta atgcactttt atctactgat ggtaacaaaa ttgccgataa gtatgtccgc 15600 aatttacaac acagacttta tgagtgtctc tatagaaata gagatgttga cacagacttt 15660 gtgaatgagt tttacgcata tttgcgtaaa catttctcaa tgatgatact ctctgacgat 15720 gctgttgtgt gtttcaatag cacttatgca tctcaaggtc tagtggctag cataaagaac 15780 tttaagtcag ttctttatta tcaaaacaat gtttttatgt ctgaagcaaa atgttggact 15840 gagactgacc ttactaaagg acctcatgaa ttttgctctc aacatacaat gctagttaaa 15900 cagggtgatg attatgtgta ccttccttac ccagatccat caagaatcct aggggccggc 15960 tgttttgtag atgatatcgt aaaaacagat ggtacactta tgattgaacg gttcgtgtct 16020 ttagctatag atgcttaccc acttactaaa catcctaatc aggagtatgc tgatgtcttt 16080 catttgtact tacaatacat aagaaagcta catgatgagt taacaggaca catgttagac 16140 atgtattctg ttatgcttac taatgataac acttcaaggt attgggaacc tgagttttat 16200 gaggctatgt acacaccgca tacagtctta caggctgttg gggcttgtgt tctttgcaat 16260 tcacagactt cattaagatg tggtgcttgc atacgtagac cattcttatg ttgtaaatgc 16320 tgttacgacc atgtcatatc aacatcacat aaattagtct tgtctgttaa tccgtatgtt 16380 tgcaatgctt caggttgtga tgtcacagat gtgactcaac tttacttagg aggtatgagc 16440 tattattgta aatcacataa accacccatt agttttccat tgtgtgctaa tggacaagtt 16500 tttggtttat ataaaaatac atgtgttggt agcgataatg ttactgactt taatgcaatt 16560 gcaacatgtg actggacaaa tgctggtgat tacattttag ctaacacctg tactgaaaga 16620 ctcaagcttt ttgcagcaga aacgctcaaa gctactgagg agacatttaa actgtcttat 16680 ggtattgcta ctgtacgtga agtgctgtct gacagagaat tacatctttc atgggaagtt 16740 ggtaaaccta gaccaccact taaccgaaat tatgtcttta ctggttatcg tgtaactaaa 16800 aacagtaaag tacaaatagg agagtacacc tttgaaaaag gtgactatgg tgatgctgtt 16860 gtttaccgag gtacaacaac ttacaaatta aatgttggtg attattttgt gctgacatca 16920 catacagtaa tgccattaag tgcacctaca ctagtgccac aagagcacta tgttagaatt 16980 actggcttat acccaacact caatatctca gatgagtttt ctagcaatgt tgcaaattat 17040 caaaaggttg gtatgcaaaa gtattctaca ctccagggac cacctggtac tggtaagagt 17100 cattttgcta ttggcctagc tctctactac ccttctgctc gcatagtgta tacagcttgc 17160 tctcatgccg ctgttgatgc actatgtgag aaggcattaa aatatttgcc tatagataaa 17220 tgtagtagaa ttatacctgc acgtgctcgt gtagagtgtt ttgataaatt caaagtgaat 17280 tcaacattag aacagtatgt cttttgtact gtaaatgcat tgcctgagac gacagcagat 17340 atagttgtct ttgatgaaat ttcaatggcc acaaattatg atttgagtgt tgtcaatgcc 17400 agattacgtg ctaagcacta tgtgtacatt ggcgaccctg ctcaattacc tgcaccacgc 17460 acattgctaa ctaagggcac actagaacca gaatatttca attcagtgtg tagacttatg 17520 aaaactatag gtccagacat gttcctcgga acttgtcggc gttgtcctgc tgaaattgtt 17580 gacactgtga gtgctttggt ttatgataat aagcttaaag cacataaaga caaatcagct 17640 caatgcttta aaatgtttta taagggtgtt atcacgcatg atgtttcatc tgcaattaac 17700 aggccacaaa taggcgtggt aagagaattc cttacacgta accctgcttg gagaaaagct 17760 gtctttattt caccttataa ttcacagaat gctgtagcct caaagatttt gggactacca 17820 actcaaactg ttgattcatc acagggctca gaatatgact atgtcatatt cactcaaacc 17880 actgaaacag ctcactcttg taatgtaaac agatttaatg ttgctattac cagagcaaaa 17940 gtaggcatac tttgcataat gtctgataga gacctttatg acaagttgca atttacaagt 18000 cttgaaattc cacgtaggaa tgtggcaact ttacaagctg aaaatgtaac aggactcttt 18060 aaagattgta gtaaggtaat cactgggtta catcctacac aggcacctac acacctcagt 18120 gttgacacta aattcaaaac tgaaggttta tgtgttgaca tacctggcat acctaaggac 18180 atgacctata gaagactcat ctctatgatg ggttttaaaa tgaattatca agttaatggt 18240 taccctaaca tgtttatcac ccgcgaagaa gctataagac atgtacgtgc atggattggc 18300 ttcgatgtcg aggggtgtca tgctactaga gaagctgttg gtaccaattt acctttacag 18360 ctaggttttt ctacaggtgt taacctagtt gctgtaccta caggttatgt tgatacacct 18420 aataatacag atttttccag agttagtgct aaaccaccgc ctggagatca atttaaacac 18480 ctcataccac ttatgtacaa aggacttcct tggaatgtag tgcgtataaa gattgtacaa 18540 atgttaagtg acacacttaa aaatctctct gacagagtcg tatttgtctt atgggcacat 18600 ggctttgagt tgacatctat gaagtatttt gtgaaaatag gacctgagcg cacctgttgt 18660 ctatgtgata gacgtgccac atgcttttcc actgcttcag acacttatgc ctgttggcat 18720 cattctattg gatttgatta cgtctataat ccgtttatga ttgatgttca acaatggggt 18780 tttacaggta acctacaaag caaccatgat ctgtattgtc aagtccatgg taatgcacat 18840 gtagctagtt gtgatgcaat catgactagg tgtctagctg tccacgagtg ctttgttaag 18900 cgtgttgact ggactattga atatcctata attggtgatg aactgaagat taatgcggct 18960 tgtagaaagg ttcaacacat ggttgttaaa gctgcattat tagcagacaa attcccagtt 19020 cttcacgaca ttggtaaccc taaagctatt aagtgtgtac ctcaagctga tgtagaatgg 19080 aagttctatg atgcacagcc ttgtagtgac aaagcttata aaatagaaga attattctat 19140 tcttatgcca cacattctga caaattcaca gatggtgtat gcctattttg gaattgcaat 19200 gtcgatagat atcctgctaa ttccattgtt tgtagatttg acactagagt gctatctaac 19260 cttaacttgc ctggttgtga tggtggcagt ttgtatgtaa ataaacatgc attccacaca 19320 ccagcttttg ataaaagtgc ttttgttaat ttaaaacaat taccattttt ctattactct 19380 gacagtccat gtgagtctca tggaaaacaa gtagtgtcag atatagatta tgtaccacta 19440 aagtctgcta cgtgtataac acgttgcaat ttaggtggtg ctgtctgtag acatcatgct 19500 aatgagtaca gattgtatct cgatgcttat aacatgatga tctcagctgg ctttagcttg 19560 tgggtttaca aacaatttga tacttataac ctctggaaca cttttacaag acttcagagt 19620 ttagaaaatg tggcttttaa tgttgtaaat aagggacact ttgatggaca acagggtgaa 19680 gtaccagttt ctatcattaa taacactgtt tacacaaaag ttgatggtgt tgatgtagaa 19740 ttgtttgaaa ataaaacaac attacctgtt aatgtagcat ttgagctttg ggctaagcgc 19800 aacattaaac cagtaccaga ggtgaaaata ctcaataatt tgggtgtgga cattgctgct 19860 aatactgtga tctgggacta caaaagagat gctccagcac atatatctac tattggtgtt 19920 tgttctatga ctgacatagc caagaaacca actgaaacga tttgtgcacc actcactgtc 19980 ttttttgatg gtagagttga tggtcaagta gacttattta gaaatgcccg taatggtgtt 20040 cttattacag aaggtagtgt taaaggttta caaccatctg taggtcccaa acaagctagt 20100 cttaatggag tcacattaat tggagaagcc gtaaaaacac agttcaatta ttataagaaa 20160 gttgatggtg ttgtccaaca attacctgaa acttacttta ctcagagtag aaatttacaa 20220 gaatttaaac ccaggagtca aatggaaatt gatttcttag aattagctat ggatgaattc 20280 attgaacggt ataaattaga aggctatgcc ttcgaacata tcgtttatgg agattttagt 20340 catagtcagt taggtggttt acatctactg attggactag ctaaacgttt taaggaatca 20400 ccttttgaat tagaagattt tattcctatg gacagtacag ttaaaaacta tttcataaca 20460 gatgcgcaaa caggttcatc taagtgtgtg tgttctgtta ttgatttatt acttgatgat 20520 tttgttgaaa taataaaatc ccaagattta tctgtagttt ctaaggttgt caaagtgact 20580 attgactata cagaaatttc atttatgctt tggtgtaaag atggccatgt agaaacattt 20640 tacccaaaat tacaatctag tcaagcgtgg caaccgggtg ttgctatgcc taatctttac 20700 aaaatgcaaa gaatgctatt agaaaagtgt gaccttcaaa attatggtga tagtgcaaca 20760 ttacctaaag gcataatgat gaatgtcgca aaatatactc aactgtgtca atatttaaac 20820 acattaacat tagctgtacc ctataatatg agagttatac attttggtgc tggttctgat 20880 aaaggagttg caccaggtac agctgtttta agacagtggt tgcctacggg tacgctgctt 20940 gtcgattcag atcttaatga ctttgtctct gatgcagatt caactttgat tggtgattgt 21000 gcaactgtac atacagctaa taaatgggat ctcattatta gtgatatgta cgaccctaag 21060 actaaaaatg ttacaaaaga aaatgactct aaagagggtt ttttcactta catttgtggg 21120 tttatacaac aaaagctagc tcttggaggt tccgtggcta taaagataac agaacattct 21180 tggaatgctg atctttataa gctcatggga cacttcgcat ggtggacagc ctttgttact 21240 aatgtgaatg cgtcatcatc tgaagcattt ttaattggat gtaattatct tggcaaacca 21300 cgcgaacaaa tagatggtta tgtcatgcat gcaaattaca tattttggag gaatacaaat 21360 ccaattcagt tgtcttccta ttctttattt gacatgagta aatttcccct taaattaagg 21420 ggtactgctg ttatgtcttt aaaagaaggt caaatcaatg atatgatttt atctcttctt 21480 agtaaaggta gacttataat tagagaaaac aacagagttg ttatttctag tgatgttctt 21540 gttaacaact aaacgaacaa tgtttgtttt tcttgtttta ttgccactag tctctagtca 21600 gtgtgttaat cttacaacca gaactcaatt accccctgca tacactaatt ctttcacacg 21660 tggtgtttat taccctgaca aagttttcag atcctcagtt ttacattcaa ctcaggactt 21720 gttcttacct ttcttttcca atgttacttg gttccatgct atacatgtct ctgggaccaa 21780 tggtactaag aggtttgcta accctgtcct accatttaat gatggtgttt attttgcttc 21840 cactgagaag tctaacataa taagaggctg gatttttggt actactttag attcgaagac 21900 ccagtcccta cttattgtta ataacgctac taatgttgtt attaaagtct gtgaatttca 21960 attttgtaat gatccatttt tgggtgttta ttaccacaaa aacaacaaaa gttggatgga 22020 aagtgagttc agagtttatt ctagtgcgaa taattgcact tttgaatatg tctctcagcc 22080 ttttcttatg gaccttgaag gaaaacaggg taatttcaaa aatcttaggg aatttgtgtt 22140 taagaatatt gatggttatt ttaaaatata ttctaagcac acgcctatta atttagtgcg 22200 tggtctccct cagggttttt cggctttaga accattggta gatttgccaa taggtattaa 22260 catcactagg tttcaaannn nnnctttaca tagaagttat ttgactcctg gtgattcttc 22320 ttcaggttgg acagctggtg ctgcagctta ttatgtgggt tatcttcaac ctaggacttt 22380 tctattaaaa tataatgaaa atggaaccat tacagatgct gtagactgtg cacttgaccc 22440 tctctcagaa acaaagtgta cgttgaaatc cttcactgta gaaaaaggaa tctatcaaac 22500 ttctaacttt agagtccaac caacagaatc tattgttaga tttcctaata ttacaaactt 22560 gtgccctttt ggtgaagttt ttaacgccac cagatttgca tctgtttatg cttggaacag 22620 gaagagaatc agcaactgtg ttgctgatta ttctgtccta tataattccg catcattttc 22680 cacttttaag tgttatggag tgtctcctac taaattaaat gatctctgct ttactaatgt 22740 ctatgcagat tcatttgtaa ttagaggtga tgaagtcaga caaatcgctc cagggcaaac 22800 tggaaatatt gctgattata attataaatt accagatgat tttacaggct gcgttatagc 22860 ttggaattct aacaatcttg attctaaggt tggtggtaat tataattacc tgtatagatt 22920 gtttaggaag tctaatctca aaccttttga gagagatatt tcaactgaaa tctatcaggc 22980 cggtagcaca ccttgtaatg gtgttaaagg ttttaattgt tactttcctt tacaatcata 23040 tggtttccaa cccacttatg gtgttggtta ccaaccatac agagtagtag tactttcttt 23100 tgaacttcta catgcaccag caactgtttg tggacctaaa aagtctacta atttggttaa 23160 aaacaaatgt gtcaatttca acttcaatgg tttaacaggc acaggtgttc ttactgagtc 23220 taacaaaaag tttctgcctt tccaacaatt tggcagagac attgctgaca ctactgatgc 23280 tgtccgtgat ccacagacac ttgagattct tgacattaca ccatgttctt ttggtggtgt 23340 cagtgttata acaccaggaa caaatacttc taaccaggtt gctgttcttt atcagggtgt 23400 taactgcaca gaagtccctg ttgctattca tgcagatcaa cttactccta cttggcgtgt 23460 ttattctaca ggttctaatg tttttcaaac acgtgcaggc tgtttaatag gggctgaaca 23520 tgtcaacaac tcatatgagt gtgacatacc cattggtgca ggtatatgcg ctagttatca 23580 gactcagact aattctcctc ggcgggcacg tagtgtagct agtcaatcca tcattgccta 23640 cactatgtca cttggtgtag aaaattcagt tgcttactct aataactcta ttgccatacc 23700 cacaaatttt actattagtg ttaccacaga aattctacca gtgtctatga ccaagacatc 23760 agtagattgt acaatgtaca tttgtggtga ttcaactgaa tgcagcaatc ttttgttgca 23820 atatggcagt ttttgtacac aattaaaccg tgctttaact ggaatagctg ttgaacaaga 23880 caaaaacacc caagaagttt ttgcacaagt caaacaaatt tacaaaacac caccaattaa 23940 agattttggt ggttttaatt tttcacaaat attaccagat ccatcaaaac caagcaagag 24000 gtcatttatt gaagatctac ttttcaacaa agtgacactt gcagatgctg gcttcatcaa 24060 acaatatggt gattgccttg gtgatattgc tgctagagac ctcatttgtg cacaaaagtt 24120 taacggcctt actgttttgc cacctttgct cacagatgaa atgattgctc aatacacttc 24180 tgcactgtta gcgggtacaa tcacttctgg ttggaccttt ggtgcaggtg ctgcattaca 24240 aataccattt gctatgcaaa tggcttatag gtttaatggt attggagtta cacagaatgt 24300 tctctatgag aaccaaaaat tgattgccaa ccaatttaat agtgctattg gcaaaattca 24360 agactcactt tcttccacag caagtgcact tggaaaactt caagatgtgg tcaaccaaaa 24420 tgcacaagct ttaaacacgc ttgttaaaca acttagctcc aattttggtg caatttcaag 24480 tgttttaaat gatatccttt cacgtcttga caaagttgag gctgaagtgc aaattgatag 24540 gttgatcaca ggcagacttc aaagtttgca gacatatgtg actcaacaat taattagagc 24600 tgcagaaatc agagcttctg ctaatcttgc tgctactaaa atgtcagagt gtgtacttgg 24660 acaatcaaaa agagttgatt tttgtggaaa gggctatcat cttatgtcct tccctcagtc 24720 agcacctcat ggtgtagtct tcttgcatgt gacttatgtc cctgcacaag aaaagaactt 24780 cacaactgct cctgccattt gtcatgatgg aaaagcacac tttcctcgtg aaggtgtctt 24840 tgtttcaaat ggcacacact ggtttgtaac acaaaggaat ttttatgaac cacaaatcat 24900 tactacagac aacacatttg tgtctggtaa ctgtgatgtt gtaataggaa ttgtcaacaa 24960 cacagtttat gatcctttgc aacctgaatt agactcattc aaggaggagt tagataaata 25020 ttttaagaat catacatcac cagatgttga tttaggtgac atctctggca ttaatgcttc 25080 agttgtaaac attcaaaaag aaattgaccg cctcaatgag gttgccaaga atttaaatga 25140 atctctcatc gatctccaag aacttggaaa gtatgagcag tatataaaat ggccatggta 25200 catttggcta ggttttatag ctggcttgat tgccatagta atggtgacaa ttatgctttg 25260 ctgtatgacc agttgctgta gttgtctcaa gggctgttgt tcttgtggat cctgctgcaa 25320 atttgatgaa gacgactctg agccagtgct caaaggagtc aaattacatt acacataaac 25380 gaacttatgg atttgtttat gagaatcttc acaattggaa ctgtaacttt gaagcaaggt 25440 gaaatcaagg atgctactcc ttcagatttt gttcgcgcta ctgcaacgat accgatacaa 25500 gcctcactcc ctttcggatg gcttattgtt ggcgttgcac ttcttgctgt ttttcatagc 25560 gcttccaaaa tcataaccct caaaaagaga tggcaactag cactctccaa gggtgttcac 25620 tttgtttgca acttgctgtt gttgtttgta acagtttact cacacctttt gctcgttgct 25680 gctggccttg aagccccttt tctctatctt tatgctttag tctacttctt gcagagtata 25740 aactttgtaa gaataataat gaggctttgg ctttgctgga aatgccgttc caaaaaccca 25800 ttactttatg atgccaacta ttttctttgc tggcatacta attgttacga ctattgtata 25860 ccttacaata gtgtaacttc ttcaattgtc attactttag gtgatggcac aacaagtcct 25920 atttctgaac atgactacca gattggtggt tatactgaaa aatgggaatc tggagtaaaa 25980 gactgtgttg tattacacag ttacttcact tcagactatt accagctgta ctcaactcaa 26040 ttgagtacag acactggtgt tgaacatgtt accttcttca tctacaataa aattgttgat 26100 gagcctgaag aacatgtcca aattcacaca atcgacggtt catccggagt tgttaatcca 26160 gtaatggaac caatttatga tgaaccgacg acgactacta gcgtgccttt gtaagcacaa 26220 gctgatgagt acgaacttat gtactcattc gtttcggaag agacaggtac gttaatagtt 26280 aatagcgtac ttctttttct tgctttcgtg gtattcttgc tagttacact agccatcctt 26340 actgcgcttc gattgtgtgc gtactgctgc aatattgtta acgtgagtct tgtaaaacct 26400 tctttttacg tttactctcg tgttaaaaat ctgaattctt ctagagttct tgatcttctg 26460 gtctaaacga actaaatatt atattagttt ttctgtttgg aactttaatt ttagccatgg 26520 cagattccaa cggtactatt accgttgaag agcttaaaaa gctccttgaa caatggaacc 26580 tagtaatagg tttcctattc cttacatgga tttgtcttct acaatttgcc tatgccaaca 26640 ggaataggtt tttgtatata attaagttaa ttttcctctg gctgttatgg ccagtaactt 26700 tagcttgttt tgtgcttgct gctgtttaca gaataaattg gatcaccggt ggaattgcta 26760 tcgcaatggc ttgtcttgta ggcttgatgt ggctcagcta cttcattgct tctttcagac 26820 tgtttgcgcg tacgcgttcc atgtggtcat tcaatccaga aactaacatt cttctcaacg 26880 tgccactcca tggcactatt ctgaccagac cgcttctaga aagtgaactc gtaatcggag 26940 ctgtgatcct tcgtggacat cttcgtattg ctggacacca tctaggacgc tgtgacatca 27000 aggacctgcc taaagaaatc actgttgcta catcacgaac gctttcttat tacaaattgg 27060 gagcttcgca gcgtgtagca ggtgactcag gttttgctgc atacagtcgc tacaggattg 27120 gcaactataa attaaacaca gaccattcca gtagcagtga caatattgct ttgcttgtac 27180 agtaagcgac aacagatgtt tcatctcgtt gactttcagg ttactatagc agagatatta 27240 ctaattatta tgaggacttt taaagtttcc atttggaatc ttgattacat cataaacctc 27300 ataattaaaa atttatctaa gtcactaact gagaataaat attctcaatt agatgaagag 27360 caaccaatgg agattgatta aacgaacatg aaaattattc ttttcttggc actgataaca 27420 ctcgctactt gtgagcttta tcactaccaa gagtgtgtta gaggtacaac agtactttta 27480 aaagaacctt gctcttctgg aacatacgag ggcaattcac catttcatcc tctagctgat 27540 aacaaatttg cactgacttg ctttagcact caatttgctt ttgcttgtcc tgacggcgta 27600 aaacacgtct atcagttacg tgccagatca gtttcaccta aactgttcat cagacaagag 27660 gaagttcaag aactttactc tccaattttt cttattgttg cggcaatagt gtttataaca 27720 ctttgcttca cactcaaaag aaagacagaa tgattgaact ttcattaatt gacttctatt 27780 tgtgcttttt agcctttctg ctattccttg ttttaattat gcttattatc ttttggttct 27840 cacttgaact gcaagatcat aatgaaactt gtcacgccta aacgaacatg aaatttcttg 27900 ttttcttagg aatcatcaca actgtagctg catttcacca agaatgtagt ttacagtcat 27960 gtactcaaca tcaaccatat gtagttgatg acccgtgtcc tattcacttc tattctaaat 28020 ggtatattag agtaggagct agaaaatcag cacctttaat tgaattgtgc gtggatgagg 28080 ctggttctaa atcacccatt cagtacatcg atatcggtaa ttatacagtt tcctgtttac 28140 cttttacaat taattgccag gaacctaaat tgggtagtct tgtagtgcgt tgttcgttct 28200 atgaagactt tttagagtat catgacgttc gtgttgtttt agattttatc taaacgaaca 28260 aactaaaatg tctgataatg gaccccaaaa tcagcgaaat gcaccccgca ttacgtttgg 28320 tggaccctca gattcaactg gcagtaacca gaatggagaa cgcagtgggg cgcgatcaaa 28380 acaacgtcgg ccccaaggtt tacccaataa tactgcgtct tggttcaccg ctctcactca 28440 acatggcaag gaagacctta aattccctcg aggacaaggc gttccaatta acaccaatag 28500 cagtccagat gaccaaattg gctactaccg aagagctacc agacgaattc gtggtggtga 28560 cggtaaaatg aaagatctca gtccaagatg gtatttctac tacctaggaa ctgggccaga 28620 agctggactt ccctatggtg ctaacaaaga cggcatcata tgggttgcaa ctgagggagc 28680 cttgaataca ccaaaagatc acattggcac ccgcaatcct gctaacaatg ctgcaatcgt 28740 gctacaactt cctcaaggaa caacattgcc aaaaggcttc tacgcagaag ggagcagagg 28800 cggcagtcaa gcctcttctc gttcctcatc acgtagtcgc aacagttcaa gaaattcaac 28860 tccaggcagc agtaggggaa tttctcctgc tagaatggct ggcaatggcg gtgatgctgc 28920 tcttgctttg ctgctgcttg acagattgaa ccagcttgag agcaaaatgt ctggtaaagg 28980 ccaacaacaa caaggccaaa ctgtcactaa gaaatctgct gctgaggctt ctaagaagcc 29040 tcggcaaaaa cgtactgcca ctaaagcata caatgtaaca caagctttcg gcagacgtgg 29100 tccagaacaa acccaaggaa attttgggga ccaggaacta atcagacaag gaactgatta 29160 caaacattgg ccgcaaattg cacaatttgc ccccagcgct tcagcgttct tcggaatgtc 29220 gcgcattggc atggaagtca caccttcggg aacgtggttg acctacacag gtgccatcaa 29280 attggatgac aaagatccaa atttcaaaga tcaagtcatt ttgctgaata agcatattga 29340 cgcatacaaa acattcccac caacagagcc taaaaaggac aaaaagaaga aggctgatga 29400 aactcaagcc ttaccgcaga gacagaagaa acagcaaact gtgactcttc ttcctgctgc 29460 agatttggat gatttctcca aacaattgca acaatccatg agcagtgctg actcaactca 29520 ggcctaaact catgcagacc acacaaggca gatgggctat ataaacgttt tcgcttttcc 29580 gtttacgata tatagtctac tcttgtgcag aatgaattct cgtaactaca tagcacaagt 29640 agatgtagtt aactttaatc tcacatagca atctttaatc agtgtgtaac attagggagg 29700 acttgaaaga gccaccacat tttcaccgag gccacgcgga gtacgatcga gtgtacagtg 29760 aacaatgcta gggagagctg cctatatgga agagccctaa tgtgtaaaat taattttagt 29820 agtgctatcc ccatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaaaa aaaaaaaaaa 29880 aaaaaaaaaa aaaaaaa 29897 <210> 19 <211> 1272 <212> PRT <213> SARS-CoV2 <220> <221> MISC_FEATURE <222> (240)..(242) <223> Xaa can be any naturally occurring amino acid <400> 19 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Xaa 225 230 235 240 Xaa Xaa Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly 245 250 255 Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg 260 265 270 Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val 275 280 285 Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser 290 295 300 Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln 305 310 315 320 Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro 325 330 335 Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp 340 345 350 Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr 355 360 365 Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr 370 375 380 Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val 385 390 395 400 Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn 405 410 415 Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val 420 425 430 Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr 435 440 445 Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu 450 455 460 Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn 465 470 475 480 Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe 485 490 495 Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu 500 505 510 Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys 515 520 525 Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly 530 535 540 Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro 545 550 555 560 Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg 565 570 575 Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly 580 585 590 Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala 595 600 605 Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His 610 615 620 Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn 625 630 635 640 Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn 645 650 655 Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser 660 665 670 Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala Ser 675 680 685 Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val 690 695 700 Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser 705 710 715 720 Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp 725 730 735 Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu 740 745 750 Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly 755 760 765 Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val 770 775 780 Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn 785 790 795 800 Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe 805 810 815 Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe 820 825 830 Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu 835 840 845 Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu 850 855 860 Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr 865 870 875 880 Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro 885 890 895 Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln 900 905 910 Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser 915 920 925 Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu 930 935 940 Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr 945 950 955 960 Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu 965 970 975 Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile 980 985 990 Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr 995 1000 1005 Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala 1010 1015 1020 Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp 1025 1030 1035 1040 Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro 1045 1050 1055 His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys 1060 1065 1070 Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His Phe 1075 1080 1085 Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val Thr 1090 1095 1100 Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe 1105 1110 1115 1120 Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val 1125 1130 1135 Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp 1140 1145 1150 Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile 1155 1160 1165 Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg 1170 1175 1180 Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln 1185 1190 1195 1200 Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp 1205 1210 1215 Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Met 1220 1225 1230 Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys Ser 1235 1240 1245 Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val Leu 1250 1255 1260 Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 20 <211> 29819 <212> DNA <213> SARS-CoV2 <400> 20 caactttcga tctcttgtag atctgttctc taaacgaact ttaaaatctg tgtggctgtc 60 actcggctgc atgcttagtg cactcacgca gtataattaa taactaatta ctgtcgttga 120 caggacacga gtaactcgtc tatcttctgc aggctgctta cggtttcgtc cgtgttgcag 180 ccgatcatca gcacatctag gttttgtccg ggtgtgaccg aaaggtaaga tggagagcct 240 tgtccctggt ttcaacgaga aaacacacgt ccaactcagt ttgcctgttt tacaggttcg 300 cgacgtgctc gtacgtggct ttggagactc cgtggaggag gtcttatcag aggcacgtca 360 acatcttaaa gatggcactt gtggcttagt agaagttgaa aaaggcgttt tgcctcaact 420 tgaacagccc tatgtgttca tcaaacgttc ggatgctcga actgcacctc atggtcatgt 480 tatggttgag ctggtagcag aactcgaagg cattcagtac ggtcgtagtg gtgagacact 540 tggtgtcctt gtccctcatg tgggcgaaat accagtggct taccgcaagg ttcttcttcg 600 taagaacggt aataaaggag ctggtggcca tagttacggc gccgatctaa agtcatttga 660 cttaggcgac gagcttggca ctgatcctta tgaagacttt caagaaaact ggaacactaa 720 acatagcagt ggtgttaccc gtgaactcat gcgtgagctt aacggagggg catacactcg 780 ctatgtcgat aacaacttct gtggccctga tggctaccct cttgagtgca ttaaagacct 840 tctagcacgt gctggtaaag cttcatgcac tttgtccgaa caactggact ttattgacac 900 taagaggggt gtatactgct gccgtgaaca tgagcatgaa attgcttggt acacggaacg 960 ttctgaaaag agctatgaat tgcagacacc ttttgaaatt aaattggcaa agaaatttga 1020 caccttcaat ggggaatgtc caaattttgt atttccctta aattccataa tcaagactat 1080 tcaaccaagg gttgaaaaga aaaagcttga tggctttatg ggtagaattc gatctgtcta 1140 tccagttgcg tcaccaaatg aatgcaacca aatgtgcctt tcaactctca tgaagtgtga 1200 tcattgtggt gaaacttcat ggcagacggg cgattttgtt aaagccactt gcgaattttg 1260 tggcactgag aatttgacta aagaaggtgc cactacttgt ggttacttac cccaaaatgc 1320 tgttgttaaa atttattgtc cagcatgtca caattcagaa gtaggacctg agcatagtct 1380 tgccgaatac cataatgaat ctggcttgaa aaccattctt cgtaagggtg gtcgcactat 1440 tgcctttgga ggctgtgtgt tctcttatgt tggttgccat aacaagtgtg cctattgggt 1500 tccacgtgct agcgctaaca taggttgtaa ccatacaggt gttgttggag aaggttccga 1560 aggtcttaat gacaaccttc ttgaaatact ccaaaaagag aaagtcaaca tcaatattgt 1620 tggtgacttt aaacttaatg aagagatcgc cattattttg gcatcttttt ctgcttccac 1680 aagtgctttt gtggaaactg tgaaaggttt ggattataaa gcattcaaac aaattgttga 1740 atcctgtggt aattttaaag ttacaaaagg aaaagctaaa aaaggtgcct ggaatattgg 1800 tgaacagaaa tcaatactga gtcctcttta tgcatttgca tcagaggctg ctcgtgttgt 1860 acgatcaatt ttctcccgca ctcttgaaac tgctcaaaat tctgtgcgtg ttttacagaa 1920 ggccgctata acaatactag atggaatttc acagtattca ctgagactca ttgatgctat 1980 gatgttcaca tctgatttgg ctactaacaa tctagttgta atggcctaca ttacaggtgg 2040 tgttgttcag ttgacttcgc agtggctaac taacatcttt ggcactgttt atgaaaaact 2100 caaacccgtc cttgattggc ttgaagagaa gtttaaggaa ggtgtagagt ttcttagaga 2160 cggttgggaa attgttaaat ttatctcaac ctgtgcttgt gaaattgtcg gtggacaaat 2220 tgtcacctgt gcaaaggaaa ttaaggagag tgttcagaca ttctttaagc ttgtaaataa 2280 atttttggct ttgtgtgctg actctatcat tattggtgga gctaaactta aagccttgaa 2340 tttaggtgaa acatttgtca cgcactcaaa gggattgtac agaaagtgtg ttaaatccag 2400 agaagaaact ggcctactca tgcctctaaa agccccaaaa gaaattatct tcttagaggg 2460 agaaacactt cccacagaag tgttaacaga ggaagttgtc ttgaaaactg gtgatttaca 2520 accattagaa caacctacta gtgaagctgt tgaagctcca ttggttggta caccagtttg 2580 tattaacggg cttatgttgc tcgaaatcaa agacacagaa aagtactgtg cccttgcacc 2640 taatatgatg gtaacaaaca ataccttcac actcaaaggc ggtgcaccaa caaaggttac 2700 ttttggtgat gatactgtga tagaagtgca aggttacaag agtgtgaata tcacttttga 2760 acttgatgaa aggattgata aagtacttaa tgagaagtgc tctgcctata cagttgaact 2820 cggtacagaa gtaaatgagt tcgcctgtgt tgtggcagat gctgtcataa aaactttgca 2880 accagtatct gaattactta caccactggg cattgattta gatgagtgga gtatggctac 2940 atactactta tttgatgagt ctggtgagtt taaattggct tcacatatgt attgttcttt 3000 ttaccctcca gatgaggatg aagaagaagg tgattgtgaa gaagaagagt ttgagccatc 3060 aactcaatat gagtatggta ctgaagatga ttaccaaggt aaacctttgg aatttggtgc 3120 cacttctgct gctcttcaac ctgaagaaga gcaagaagaa gattggttag atgatgatag 3180 tcaacaaact gttggtcaac aagacggcag tgaggacaat cagacaacta ctattcaaac 3240 aattgttgag gttcaacctc aattagagat ggaacttaca ccagttgttc agactattga 3300 agtgaatagt tttagtggtt atttaaaact tactgacaat gtatacatta aaaatgcaga 3360 cattgtggaa gaagctaaaa aggtaaaacc aacagtggtt gttaatgcag ccaatgttta 3420 ccttaaacat ggaggaggtg ttgcaggagc cttaaataag gctactaaca atgccatgca 3480 agttgaatct gatgattaca tagctactaa tggaccactt aaagtgggtg gtagttgtgt 3540 tttaagcgga cacaatcttg ctaaacactg tcttcatgtt gtcggcccaa atgttaacaa 3600 aggtgaagac attcaacttc ttaagagtgc ttatgaaaat tttaatcagc acgaagttct 3660 acttgcacca ttattatcag ctggtatttt tggtgctgac cctatacatt ctttaagagt 3720 ttgtgtagat actgttcgca caaatgtcta cttagctgtc tttgataaaa atctctatga 3780 caaacttgtt ttaagctttt tggaaatgaa gagtgaaaag caagttgaac aaaagatcgc 3840 tgagattcct aaagaggaag ttaagccatt tataactgaa agtaaacctt cagttgaaca 3900 gagaaaacaa gatgataaga aaatcaaagc ttgtgttgaa gaagttacaa caactctgga 3960 agaaactaag ttcctcacag aaaacttgtt actttatatt gacattaatg gcaatcttca 4020 tccagattct gccactcttg ttagtgacat tgacatcact ttcttaaaga aagatgctcc 4080 atatatagtg ggtgatgttg ttcaagaggg tgttttaact gctgtggtta tacctactaa 4140 aaaggctggt ggcactactg aaatgctagc gaaagctttg agaaaagtgc caacagacaa 4200 ttatataacc acttacccgg gtcagggttt aaatggttac actgtagagg aggcaaagac 4260 agtgcttaaa aagtgtaaaa gtgcctttta cattctacca tctattatct ctaatgagaa 4320 gcaagaaatt cttggaactg tttcttggaa tttgcgagaa atgcttgcac atgcagaaga 4380 aacacgcaaa ttaatgcctg tctgtgtgga aactaaagcc atagtttcaa ctatacagcg 4440 taaatataag ggtattaaaa tacaagaggg tgtggttgat tatggtgcta gattttactt 4500 ttacaccagt aaaacaactg tagcgtcact tatcaacaca cttaacgatc taaatgaaac 4560 tcttgttaca atgccacttg gctatgtaac acatggctta aatttggaag aagctgctcg 4620 gtatatgaga tctctcaaag tgccagctac agtttctgtt tcttcacctg atgctgttac 4680 agcgtataat ggttatctta cttcttcttc taaaacacct gaagaacatt ttattgaaac 4740 catctcactt gctggttcct ataaagattg gtcctattct ggacaatcta cacaactagg 4800 tatagaattt cttaagagag gtgataaaag tgtatattac actagtaatc ctaccacatt 4860 ccacctagat ggtgaagtta tcacctttga caatcttaag acacttcttt ctttgagaga 4920 agtgaggact attaaggtgt ttacaacagt agacaacatt aacctccaca cgcaagttgt 4980 ggacatgtca atgacatatg gacaacagtt tggtccaact tatttggatg gagctgatgt 5040 tactaaaata aaacctcata attcacatga aggtaaaaca ttttatgttt tacctaatga 5100 tgacactcta cgtgttgagg cttttgagta ctaccacaca actgatccta gttttctggg 5160 taggtacatg tcagcattaa atcacactaa aaagtggaaa tacccacaag ttaatggttt 5220 aacttctatt aaatgggcag ataacaactg ttatcttgcc actgcattgt taacactcca 5280 acaaatagag ttgaagttta atccacctgc tctacaagat gcttattaca gagcaagggc 5340 tggtgaagct gctaactttt gtgcacttat cttagcctac tgtaataaga cagtaggtga 5400 gttaggtgat gttagagaaa caatgagtta cttgtttcaa catgccaatt tagattcttg 5460 caaaagagtc ttgaacgtgg tgtgtaaaac ttgtggacaa cagcagacaa cccttaaggg 5520 tgtagaagct gttatgtaca tgggcacact ttcttatgaa caatttaaga aaggtgttca 5580 gataccttgt acgtgtggta aacaagctac acaatatcta gtacaacagg agtcaccttt 5640 tgttatgatg tcagcaccac ctgctcagta tgaacttaag catggtacat ttacttgtgc 5700 tagtgagtac actggtaatt accagtgtgg tcactataaa catataactt ctaaagaaac 5760 tttgtattgc atagacggtg ctttacttac aaagtcctca gaatacaaag gtcctattac 5820 ggatgttttc tacaaagaaa acagttacac aacaaccata aaaccagtta cttataaatt 5880 ggatggtgtt gtttgtacag aaattgaccc taagttggac aattattata agaaagacaa 5940 ttcttatttc acagagcaac caattgatct tgtaccaaac caaccatatc caaacgcaag 6000 cttcgataat tttaagtttg tatgtgataa tatcaaattt gctgatgatt taaaccagtt 6060 aactggttat aagaaacctg cttcaagaga gcttaaagtt acatttttcc ctgacttaaa 6120 tggtgatgtg gtggctattg attataaaca ctacacaccc tcttttaaga aaggagctaa 6180 attgttacat aaacctattg tttggcatgt taacaatgca actaataaag ccacgtataa 6240 accaaatacc tggtgtatac gttgtctttg gagcacaaaa ccggttgaaa catcaaattc 6300 gtttgatgta ctgaagtcag aggacgcgca gggaatggat aatcttgcct gcgaagatct 6360 aaaaccagtc tctgaagaag tagtggaaaa tcctaccata cagaaagacg ttcttgagtg 6420 taatgtgaaa actaccgaag ttgtaggaga cattatactt aaaccagcaa ataatagttt 6480 aaaaattaca gaagaggttg gccacacaga tctaatggct gcttatgtag acaattctag 6540 tcttactatt aagaaaccta atgaattatc tagagtgtta ggtttgaaaa cccttgctac 6600 tcatggttta gctgctgtta atagtgtccc ttgggatact atagctaatt atgctaagcc 6660 ttttcttaac aaagttgtta gtacaactac taacatagtt acacggtgtt taaaccgtgt 6720 ttgtactaat tatatgcctt atttctttac tttattgcta caattgtgta cttttactag 6780 aagtacaaat tctagaatta aagcatctat gccgactact atagcaaaga atactgttaa 6840 gagtgtcggt aaattttgtc tagaggcttc atttaattat ttgaagtcac ctaatttttc 6900 taaactgata aatattataa tttggttttt actattaagt gtttgcctag gttctttaat 6960 ctactcaacc gctgctttag gtgttttaat gtctaattta ggcatgcctt cttactgtac 7020 tggttacaga gaaggctatt tgaactctac taatgtcact attgcaacct actgtactgg 7080 ttctatacct tgtagtgttt gtcttagtgg tttagattct ttagacacct atccttcttt 7140 agaaactata caaattacca tttcatcttt taaatgggat ttaactgctt ttggcttagt 7200 tgcagagtgg tttttggcat atattctttt cactaggttt ttctatgtac ttggattggc 7260 tgcaatcatg caattgtttt tcagctattt tgcagtacat tttattagta attcttggct 7320 tatgtggtta ataattaatc ttgtacaaat ggccccgatt tcagctatgg ttagaatgta 7380 catcttcttt gcatcatttt attatgtatg gaaaagttat gtgcatgttg tagacggttg 7440 taattcatca acttgtatga tgtgttacaa acgtaataga gcaacaagag tcgaatgtac 7500 aactattgtt aatggtgtta gaaggtcctt ttatgtctat gctaatggag gtaaaggctt 7560 ttgcaaacta cacaattgga attgtgttaa ttgtgataca ttctgtgctg gtagtacatt 7620 tattagtgat gaagttgcga gagacttgtc actacagttt aaaagaccaa taaatcctac 7680 tgaccagtct tcttacatcg ttgatagtgt tacagtgaag aatggttcca tccatcttta 7740 ctttgataaa gctggtcaaa agacttatga aagacattct ctctctcatt ttgttaactt 7800 agacaacctg agagctaata acactaaagg ttcattgcct attaatgtta tagtttttga 7860 tggtaaatca aaatgtgaag aatcatctgc aaaatcagcg tctgtttact acagtcagct 7920 tatgtgtcaa cctatactgt tactagatca ggcattagtg tctgatgttg gtgatagtgc 7980 ggaagttgca gttaaaatgt ttgatgctta cgttaatacg ttttcatcaa cttttaacgt 8040 accaatggaa aaactcaaaa cactagttgc aactgcagaa gctgaacttg caaagaatgt 8100 gtccttagac aatgtcttat ctacttttat ttcagcagct cggcaagggt ttgttgattc 8160 agatgtagaa actaaagatg ttgttgaatg tcttaaattg tcacatcaat ctgacataga 8220 agttactggc gatagttgta ataactatat gctcacctat aacaaagttg aaaacatgac 8280 accccgtgac cttggtgctt gtattgactg tagtgcgcgt catattaatg cgcaggtagc 8340 aaaaagtcac aacattgctt tgatatggaa cgttaaagat ttcatgtcat tgtctgaaca 8400 actacgaaaa caaatacgta gtgctgctaa aaagaataac ttacctttta agttgacatg 8460 tgcaactact agacaagttg ttaatgttgt aacaacaaag atagcactta agggtggtaa 8520 aattgttaat aattggttga agcagttaat taaagttaca cttgtgttcc tttttgttgc 8580 tgctattttc tatttaataa cacctgttca tgtcatgtct aaacatactg acttttcaag 8640 tgaaatcata ggatacaagg ctattgatgg tggtgtcact cgtgacatag catctacaga 8700 tacttgtttt gctaacaaac atgctgattt tgacacatgg tttagccagc gtggtggtag 8760 ttatactaat gacaaagctt gcccattgat tgctgcagtc ataacaagag aagtgggttt 8820 tgtcgtgcct ggtttgcctg gcacgatatt acgcacaact aatggtgact ttttgcattt 8880 cttacctaga gtttttagtg cagttggtaa catctgttac acaccatcaa aacttataga 8940 gtacactgac tttgcaacat cagcttgtgt tttggctgct gaatgtacaa tttttaaaga 9000 tgcttctggt aagccagtac catattgtta tgataccaat gtactagaag gttctgttgc 9060 ttatgaaaat ttacgccctg acacacgtta tgtgctcatg gatggctcta ttattcaatt 9120 tcctaacacc taccttgaag gttctgttag agtggtaaca acttttgatt ctgagtactg 9180 taggcacggc acttgtgaaa gatcagaagc tggtgtttgt gtatctacta gtggtagatg 9240 ggtacttaac aatgattatt acagatcttt accaggagtt ttctgtggtg tagatgctgt 9300 aaatttactt actaatatgt ttacaccact aattcaacct attggtgctt tggacatatc 9360 agcatctata gtagctggtg gtattgtagc tatcgtagta acatgccttg cctactattt 9420 tatgaggttt agaagagctt ttggtgaata cagtcatgta gttgccttta atactttact 9480 attccttatg tcattcactg tactctgttt aacaccagtt tactcattct tacctggtgt 9540 ttattctgtt atttacttgt acttgacatt ttatcttact aatgatgttt cttttttagc 9600 acatattcag tggatggtta tgttcacacc tttagtacct ttctggataa caattgctta 9660 tatcatttgt atttccacaa agcatttcta ttggttcttt agtaattacc taaagagacg 9720 tgtagtcttt aatggtgttt cctttagtac ttttgaagaa gctgcgctgt gcaccttttt 9780 gttaaataaa gaaatgtatc taaagttgcg tagtgatgtg ctattacctc ttacgcaata 9840 taatagatac ttagctcttt ataataagta caagtatttt agtggagcaa tggatacaac 9900 tagctacaga gaagctgctt gttgtcatct cgcaaaggct ctcaatgact tcagtaactc 9960 aggttctgat gttctttacc aaccaccaca aacctctatc acctcagctg ttttgcagag 10020 tggttttaga aaaatggcat tcccatctgg taaagttgag ggttgtatgg tacaagtaac 10080 ttgtggtaca actacactta acggtctttg gcttgatgac gtagtttact gtccaagaca 10140 tgtgatctgc acctctgaag acatgcttaa ccctaattat gaagatttac tcattcgtaa 10200 gtctaatcat aatttcttgg tacaggctgg taatgttcaa ctcagggtta ttggacattc 10260 tatgcaaaat tgtgtactta agcttaaggt tgatacagcc aatcctaaga cacctaagta 10320 taagtttgtt cgcattcaac caggacagac tttttcagtg ttagcttgtt acaatggttc 10380 accatctggt gtttaccaat gtgctatgag gcccaatttc actattaagg gttcattcct 10440 taatggttca tgtggtagtg ttggttttaa catagattat gactgtgtct ctttttgtta 10500 catgcaccat atggaattac caactggagt tcatgctggc acagacttag aaggtaactt 10560 ttatggacct tttgttgaca ggcaaacagc acaagcagct ggtacggaca caactattac 10620 agttaatgtt ttagcttggt tgtacgctgc tgttataaat ggagacaggt ggtttctcaa 10680 tcgatttacc acaactctta atgactttaa ccttgtggct atgaagtaca attatgaacc 10740 tctaacacaa gaccatgttg acatactagg acctctttct gctcaaactg gaattgtcgt 10800 tttagatatg tgtgcttcat taaaagaatt actgcaaaat ggtatgaatg gacgtaccat 10860 attgggtagt gctttattag aagatgaatt tacacctttt gatgttgtta gacaatgctc 10920 aggtgttact ttccaaagtg cagtgaaaag aacaatcaag ggtacacacc actggttgtt 10980 actcacaatt ttgacttcac ttttagtttt agtccagagt actcaatggt ctttgttctt 11040 ttttttgtat gaaaatgcct ttttaccttt tgctatgggt attattgcta tgtctgcttt 11100 tgcaatgatg tttgtcaaac ataagcatgc atttctctgt ttgtttttgt taccttctct 11160 tgccactgta gcttatttta atatggtcta tatgcctgct agttgggtga tgcgtattat 11220 gacatggttg gatatggttg atactagttt gaagctaaaa gactgtgtta tgtatgcatc 11280 agctgtagtg ttactaatcc ttatgacagc aagaactgtg tatgatgatg gtgctaggag 11340 agtgtggaca cttatgaatg tcttgacact cgtttataaa gtttattatg gtaatgcttt 11400 agatcaagcc atttccatgt gggctcttat aatctctgtt acttctaact actcaggtgt 11460 agttacaact gtcatgtttt tggccagagg tattgttttt atgtgtgttg agtattgccc 11520 tattttcttc ataactggta atacacttca gtgtataatg ctagtttatt gtttcttagg 11580 ctatttttgt acttgttact ttggcctctt ttgtttactc aaccgctact ttagactgac 11640 tcttggtgtt tatgattact tagtttctac acaggagttt agatatatga attcacaggg 11700 actactccca cccaagaata gcatagatgc cttcaaactc aacattaaat tgttgggtgt 11760 tggtggcaaa ccttgtatca aagtagccac tgtacagtct aaaatgtcag atgtaaagtg 11820 cacatcagta gtcttactct cagttttgca acaactcaga gtagaatcat catctaaatt 11880 gtgggctcaa tgtgtccagt tacacaatga cattctctta gctaaagata ctactgaagc 11940 ctttgaaaaa atggtttcac tactttctgt tttgctttcc atgcagggtg ctgtagacat 12000 aaacaagctt tgtgaagaaa tgctggacaa cagggcaacc ttacaagcta tagcctcaga 12060 gtttagttcc cttccatcat atgcagcttt tgctactgct caagaagctt atgagcaggc 12120 tgttgctaat ggtgattctg aagttgttct taaaaagttg aagaagtctt tgaatgtggc 12180 taaatctgaa tttgaccgtg atgcagccat gcaacgtaag ttggaaaaga tggctgatca 12240 agctatgacc caaatgtata aacaggctag atctgaggac aagagggcaa aagttactag 12300 tgctatgcag acaatgcttt tcactatgct tagaaagttg gataatgatg cactcaacaa 12360 cattatcaac aatgcaagag atggttgtgt tcccttgaac ataatacctc ttacaacagc 12420 agccaaacta atggttgtca taccagacta taacacatat aaaaatacgt gtgatggtac 12480 aacatttact tatgcatcag cattgtggga aatccaacag gttgtagatg cagatagtaa 12540 aattgttcaa cttagtgaaa ttagtatgga caattcacct aatttagcat ggcctcttat 12600 tgtaacagct ttaagggcca attctgctgt caaattacag aataatgagc ttagtcctgt 12660 tgcactacga cagatgtctt gtgctgccgg tactacacaa actgcttgca ctgatgacaa 12720 tgcgttagct tattacaaca caacaaaggg aggtaggttt gtacttgcac tgttatccga 12780 tttacaggat ttgaaatggg ctagattccc taagagtgat ggaactggta ctatctatac 12840 agaactggaa ccaccttgta ggtttgttac agacacacct aaaggtccta aagtgaagta 12900 tttatacttt attaaaggat taaacaacct aaatagaggt atggtacttg gtagtttagc 12960 tgccacagta cgtctacaag ctggtaatgc aacagaagtg cctgccaatt caactgtatt 13020 atctttctgt gcttttgctg tagatgctgc taaagcttac aaagattatc tagctagtgg 13080 gggacaacca atcactaatt gtgttaagat gttgtgtaca cacactggta ctggtcaggc 13140 aataacagtt acaccggaag ccaatatgga tcaagaatcc tttggtggtg catcgtgttg 13200 tctgtactgc cgttgccaca tagatcatcc aaatcctaaa ggattttgtg acttaaaagg 13260 taagtatgta caaataccta caacttgtgc taatgaccct gtgggtttta cacttaaaaa 13320 cacagtctgt accgtctgcg gtatgtggaa aggttatggc tgtagttgtg atcaactccg 13380 cgaacccatg cttcagtcag ctgatgcaca atcgttttta aacgggtttg cggtgtaagt 13440 gcagcccgtc ttacaccgtg cggcacaggc actagtactg atgtcgtata cagggctttt 13500 gacatctaca atgataaagt agctggtttt gctaaattcc taaaaactaa ttgttgtcgc 13560 ttccaagaaa aggacgaaga tgacaattta attgattctt actttgtagt taagagacac 13620 actttctcta actaccaaca tgaagaaaca atttataatt tacttaagga ttgtccagct 13680 gttgctaaac atgacttctt taagtttaga atagacggtg acatggtacc acatatatca 13740 cgtcaacgtc ttactaaata cacaatggca gacctcgtct atgctttaag gcattttgat 13800 gaaggtaatt gtgatacatt aaaagaaata cttgtcacat acaattgttg tgatgatgat 13860 tatttcaata aaaaggactg gtatgatttt gtagaaaacc cagatatatt acgcgtatac 13920 gccaacttag gtgaacgtgt acgccaagct ttgttaaaaa cagtacaatt ctgtgatgcc 13980 atgcgaaatg ctggtattgt tggtgtactg acattagata atcaagatct caatggtaac 14040 tggtatgatt tcggtgattt catacaaacc acgccaggta gtggagttcc tgttgtagat 14100 tcttattatt cattgttaat gcctatatta accttgacca gggctttaac tgcagagtca 14160 catgttgaca ctgacttaac aaagccttac attaagtggg atttgttaaa atatgacttc 14220 acggaagaga ggttaaaact ctttgaccgt tattttaaat attgggatca gacataccac 14280 ccaaattgtg ttaactgttt ggatgacaga tgcattctgc attgtgcaaa ctttaatgtt 14340 ttattctcta cagtgttccc acttacaagt tttggaccac tagtgagaaa aatatttgtt 14400 gatggtgttc catttgtagt ttcaactgga taccacttca gagagctagg tgttgtacat 14460 aatcaggatg taaacttaca tagctctaga cttagtttta aggaattact tgtgtatgct 14520 gctgaccctg ctatgcacgc tgcttctggt aatctattac tagataaacg cactacgtgc 14580 ttttcagtag ctgcacttac taacaatgtt gcttttcaaa ctgtcaaacc cggtaatttt 14640 aacaaagact tctatgactt tgctgtgtct aagggtttct ttaaggaagg aagttctgtt 14700 gaattaaaac acttcttctt tgctcaggat ggtaatgctg ctatcagcga ttatgactac 14760 tatcgttata atctaccaac aatgtgtgat atcagacaac tactatttgt agttgaagtt 14820 gttgataagt actttgattg ttacgatggt ggctgtatta atgctaacca agtcatcgtc 14880 aacaacctag acaaatcagc tggttttcca tttaataaat ggggtaaggc tagactttat 14940 tatgattcaa tgagttatga ggatcaagat gcacttttcg catatacaaa acgtaatgtc 15000 atccctacta taactcaaat gaatcttaag tatgccatta gtgcaaagaa tagagctcgc 15060 accgtagctg gtgtctctat ctgtagtact atgaccaata gacagtttca tcaaaaatta 15120 ttgaaatcaa tagccgccac tagaggagct actgtagtaa ttggaacaag caaattctat 15180 ggtggttggc acaacatgtt aaaaactgtt tatagtgatg tagaaaaccc tcaccttatg 15240 ggttgggatt atcctaaatg tgatagagcc atgcctaaca tgcttagaat tatggcctca 15300 cttgttcttg ctcgcaaaca tacaacgtgt tgtagcttgt cacaccgttt ctatagatta 15360 gctaatgagt gtgctcaagt attgagtgaa atggtcatgt gtggcggttc actatatgtt 15420 aaaccaggtg gaacctcatc aggagatgcc acaactgctt atgctaatag tgtttttaac 15480 atttgtcaag ctgtcacggc caatgttaat gcacttttat ctactgatgg taacaaaatt 15540 gccgataagt atgtccgcaa tttacaacac agactttatg agtgtctcta tagaaataga 15600 gatgttgaca cagactttgt gaatgagttt tacgcatatt tgcgtaaaca tttctcaatg 15660 atgatactct ctgacgatgc tgttgtgtgt ttcaatagca cttatgcatc tcaaggtcta 15720 gtggctagca taaagaactt taagtcagtt ctttattatc aaaacaatgt ttttatgtct 15780 gaagcaaaat gttggactga gactgacctt actaaaggac ctcatgaatt ttgctctcaa 15840 catacaatgc tagttaaaca gggtgatgat tatgtgtacc ttccttaccc agatccatca 15900 agaatcctag gggccggctg ttttgtagat gatatcgtaa aaacagatgg tacacttatg 15960 attgaacggt tcgtgtcttt agctatagat gcttacccac ttactaaaca tcctaatcag 16020 gagtatgctg atgtctttca tttgtactta caatacataa gaaagctaca tgatgagtta 16080 acaggacaca tgttagacat gtattctgtt atgcttacta atgataacac ttcaaggtat 16140 tgggaacctg agttttatga ggctatgtac acaccgcata cagtcttaca ggctgttggg 16200 gcttgtgttc tttgcaattc acagacttca ttaagatgtg gtgcttgcat acgtagacca 16260 ttcttatgtt gtaaatgctg ttacgaccat gtcatatcaa catcacataa attagtcttg 16320 tctgttaatc cgtatgtttg caatgctcca ggttgtgatg tcacagatgt gactcaactt 16380 tacttaggag gtatgagcta ttattgtaaa tcacataaac cacccattag ttttccattg 16440 tgtgctaatg gacaagtttt tggtttatat aaaaatacat gtgttggtag cgataatgtt 16500 actgacttta atgcaattgc aacatgtgac tggacaaatg ctggtgatta cattttagct 16560 aacacctgta ctgaaagact caagcttttt gcagcagaaa cgctcaaagc tactgaggag 16620 acatttaaac tgtcttatgg tattgctact gtacgtgaag tgctgtctga cagagaatta 16680 catctttcat gggaagttgg taaacctaga ccaccactta accgaaatta tgtctttact 16740 ggttatcgtg taactaaaaa cagtaaagta caaataggag agtacacctt tgaaaaaggt 16800 gactatggtg atgctgttgt ttaccgaggt acaacaactt acaaattaaa tgttggtgat 16860 tattttgtgc tgacatcaca tacagtaatg ccattaagtg cacctacact agtgccacaa 16920 gagcactatg ttagaattac tggcttatac ccaacactca atatctcaga tgagttttct 16980 agcaatgttg caaattatca aaaggttggt atgcaaaagt attctacact ccagggacca 17040 cctggtactg gtaagagtca ttttgctatt ggcctagctc tctactaccc ttctgctcgc 17100 atagtgtata cagcttgctc tcatgccgct gttgatgcac tatgtgagaa ggcattaaaa 17160 tatttgccta tagataaatg tagtagaatt atacctgcac gtgctcgtgt agattgtttt 17220 gataaattca aagtgaattc aacattagaa cagtatgtct tttgtactgt aaatgcattg 17280 cctgagacga cagcagatat agttgtcttt gatgaaattt caatggccac aaattatgat 17340 ttgagtgttg tcaatgccag attacgtgct aagcactatg tgtacattgg cgaccctgct 17400 caattacctg caccacgcac attgctaact aagggcacac tagaaccaga atatttcaat 17460 tcagtgtgta gacttatgaa aactataggt ccagacatgt tcctcggaac ttgtcggcgt 17520 tgtcctgctg aaattgttga cactgtgagt gctttggttt atgataataa gcttaaagca 17580 cataaagaca aatcagctca atgctttaaa atgttttata agggtgttat cacgcatgat 17640 gtttcatctg caattaacag gccacaaata ggcgtggtaa gagaattcct tacacgtaac 17700 cctgcttgga gaaaagctgt ctttatttca ccttataatt cacagaatgc tgtagcctca 17760 aagattttgg gactaccaac tcaaactgtt gattcatcac agggctcaga atatgactat 17820 gtcatattca ctcaaaccac tgaaacagct cactcttgta atgtaaacag atttaatgtt 17880 gctattacca gagcaaaagt aggcatactt tgcataatgt ctgatagaga cctttatgac 17940 aagttgcaat ttacaagtct tgaaattcca cgtaggaatg tggcaacttt acaagctgaa 18000 aatgtaacag gactctttaa agattgtagt aaggtaatca ctgggttaca tcctacacag 18060 gcacctacac acctcagtgt tgacactaaa ttcaaaactg aaggtttatg tgttgacata 18120 cctggcatac ctaaggacat gacctataga agactcatct ctatgatggg ttttaaaatg 18180 aattatcaag ttaatggtta ccctaacatg tttatcaccc gcgaagaagc tataagacat 18240 gtacgtgcat ggattggctt cgatgtcgag gggtgtcatg ctactagaga agctgttggt 18300 accaatttac ctttacagct aggtttttct acaggtgtta acctagttgc tgtacctaca 18360 ggttatgttg atacacctaa taatacagat ttttccagag ttagtgctaa accaccgcct 18420 ggagatcaat ttaaacacct cataccactt atgtacaaag gacttccttg gaatgtagtg 18480 cgtataaaga ttgtacaaat gttaagtgac acacttaaaa atctctctga cagagtcgta 18540 tttgtcttat gggcacatgg ctttgagttg acatctatga agtattttgt gaaaatagga 18600 cctgagcgca cctgttgtct atgtgataga cgtgccacat gcttttccac tgcttcagac 18660 acttatgcct gttggcatca ttctattgga tttgattacg tctataatcc gtttatgatt 18720 gatgttcaac aatggggttt tacaggtaac ctacaaagca accatgatct gtattgtcaa 18780 gtccatggta atgcacatgt agctagttgt gatgcaatca tgactaggtg tctagctgtc 18840 cacgagtgct ttgttaagcg tgttgactgg actattgaat atcctataat tggtgatgaa 18900 ctgaagatta atgcggcttg tagaaaggtt caacacatgg ttgttaaagc tgcattatta 18960 gcagacaaat tcccagttct tcacgacatt ggtaacccta aagctattaa gtgtgtacct 19020 caagctgatg tagaatggaa gttctatgat gcacagcctt gtagtgacaa agcttataaa 19080 atagaagaat tattctattc ttatgccaca cattctgaca aattcacaga tggtgtatgc 19140 ctattttgga attgcaatgt cgatagatat cctgctaatt ccattgtttg tagatttgac 19200 actagagtgc tatctaacct taacttgcct ggttgtgatg gtggcagttt gtatgtaaat 19260 aaacatgcat tccacacacc agcttttgat aaaagtgctt ttgttaattt aaaacaatta 19320 ccatttttct attactctga cagtccatgt gagtctcatg gaaaacaagt agtgtcagat 19380 atagattatg taccactaaa gtctgctacg tgtataacac gttgcaattt aggtggtgct 19440 gtctgtagac atcatgctaa tgagtacaga ttgtatctcg atgcttataa catgatgatc 19500 tcagctggct ttagcttgtg ggtttacaaa caatttgata cttataacct ctggaacact 19560 tttacaagac ttcagagttt agaaaatgtg gcttttaatg ttgtaaataa gggacacttt 19620 gatggacaac agggtgaagt accagtttct atcattaata acactgttta cacaaaagtt 19680 gatggtgttg atgtagaatt gtttgaaaat aaaacaacat tacctgttaa tgtagcattt 19740 gagctttggg ctaagcgcaa cattaaacca gtaccagagg tgaaaatact caataatttg 19800 ggtgtggaca ttgctgctaa tactgtgatc tgggactaca aaagagatgc tccagcacat 19860 atatctacta ttggtgtttg ttctatgact gacatagcca agaaaccaac tgaaacgatt 19920 tgtgcaccac tcactgtctt ttttgatggt agagttgatg gtcaagtaga cttatttaga 19980 aatgcccgta atggtgttct tattacagaa ggtagtgtta aaggtttaca accatctgta 20040 ggtcccaaac aagctagtct taatggagtc acattaattg gagaagccgt aaaaacacag 20100 ttcaattatt ataagaaagt tgatggtgtt gtccaacaat tacctgaaac ttactttact 20160 cagagtagaa atttacaaga atttaaaccc aggagtcaaa tggaaattga tttcttagaa 20220 ttagctatgg atgaattcat tgaacggtat aaattagaag gctatgcctt cgaacatatc 20280 gtttatggag attttagtca tagtcagtta ggtggtttac atctactgat tggactagct 20340 aaacgtttta aggaatcacc ttttgaatta gaagatttta ttcctatgga cagtacagtt 20400 aaaaactatt tcataacaga tgcgcaaaca ggttcatcta agtgtgtgtg ttctgttatt 20460 gatttattac ttgatgattt tgttgaaata ataaaatccc aagatttatc tgtagtttct 20520 aaggttgtca aagtgactat tgactataca gaaatttcat ttatgctttg gtgtaaagat 20580 ggccatgtag aaacatttta cccaaaatta caatctagtc aagcgtggca accgggtgtt 20640 gctatgccta atctttacaa aatgcaaaga atgctattag aaaagtgtga ccttcaaaat 20700 tatggtgata gtgcaacatt acctaaaggc ataatgatga atgtcgcaaa atatactcaa 20760 ctgtgtcaat atttaaacac attaacatta gctgtaccct ataatatgag agttatacat 20820 tttggtgctg gttctgataa aggagttgca ccaggtacag ctgttttaag acagtggttg 20880 cctacgggta cgctgcttgt cgattcagat cttaatgact ttgtctctga tgcagattca 20940 actttgattg gtgattgtgc aactgtacat acagctaata aatgggatct cattattagt 21000 gatatgtacg accctaagac taaaaatgtt acaaaagaaa atgactctaa agagggtttt 21060 ttcacttaca tttgtgggtt tatacaacaa aagctagctc ttggaggttc cgtggctata 21120 aagataacag aacattcttg gaatgctgat ctttataagc tcatgggaca cttcgcatgg 21180 tggacagcct ttgttactaa tgtgaatgcg tcatcatctg aagcattttt aattggatgt 21240 aattatcttg gcaaaccacg cgaacaaata gatggttatg tcatgcatgc aaattacata 21300 ttttggagga atacaaatcc aattcagttg tcttcctatt ctttatttga catgagtaaa 21360 tttcccctta aattaagggg tactgctgtt atgtctttaa aagaaggtca aatcaatgat 21420 atgattttat ctcttcttag taaaggtaga cttataatta gagaaaacaa cagagttgtt 21480 atttctagtg atgttcttgt taacaactaa acgaacaatg tttgtttttc ttgttttatt 21540 gccactagtc tctagtcagt gtgttaattt tacaaacaga actcaattac cctctgcata 21600 cactaattct ttcacacgtg gtgtttatta ccctgacaaa gttttcagat cctcagtttt 21660 acattcaact caggacttgt tcttaccttt cttttccaat gttacttggt tccatgctat 21720 acatgtctct gggaccaatg gtactaagag gtttgataac cctgtcctac catttaatga 21780 tggtgtttat tttgcttcca ctgagaagtc taacataata agaggctgga tttttggtac 21840 tactttagat tcgaagaccc agtccctact tattgttaat aacgctacta atgttgttat 21900 taaagtctgt gaatttcaat tttgtaatta tccatttttg ggtgtttatt accacaaaaa 21960 caacaaaagt tggatggaaa gtgagttcag agtttattct agtgcgaata attgcacttt 22020 tgaatatgtc tctcagcctt ttcttatgga ccttgaagga aaacagggta atttcaaaaa 22080 tcttagtgaa tttgtgttta agaatattga tggttatttt aaaatatatt ctaagcacac 22140 gcctattaat ttagtgcgtg atctccctca gggtttttcg gctttagaac cattggtaga 22200 tttgccaata ggtattaaca tcactaggtt tcaaacttta cttgctttac atagaagtta 22260 tttgactcct ggtgattctt cttcaggttg gacagctggt gctgcagctt attatgtggg 22320 ttatcttcaa cctaggactt ttctattaaa atataatgaa aatggaacca ttacagatgc 22380 tgtagactgt gcacttgacc ctctctcaga aacaaagtgt acgttgaaat ccttcactgt 22440 agaaaaagga atctatcaaa cttctaactt tagagtccaa ccaacagaat ctattgttag 22500 atttcctaat attacaaact tgtgcccttt tggtgaagtt tttaacgcca ccagatttgc 22560 atctgtttat gcttggaaca ggaagagaat cagcaactgt gttgctgatt attctgtcct 22620 atataattcc gcatcatttt ccacttttaa gtgttatgga gtgtctccta ctaaattaaa 22680 tgatctctgc tttactaatg tctatgcaga ttcatttgta attagaggtg atgaagtcag 22740 acaaatcgct ccagggcaaa ctggaacgat tgctgattat aattataaat taccagatga 22800 ttttacaggc tgcgttatag cttggaattc taacaatctt gattctaagg ttggtggtaa 22860 ttataattac ctgtatagat tgtttaggaa gtctaatctc aaaccttttg agagagatat 22920 ttcaactgaa atctatcagg ccggtagcac accttgtaat ggtgttaaag gttttaattg 22980 ttactttcct ttacaatcat atggtttcca acccacttat ggtgttggtt accaaccata 23040 cagagtagta gtactttctt ttgaacttct acatgcacca gcaactgttt gtggacctaa 23100 aaagtctact aatttggtta aaaacaaatg tgtcaatttc aacttcaatg gtttaacagg 23160 cacaggtgtt cttactgagt ctaacaaaaa gtttctgcct ttccaacaat ttggcagaga 23220 cattgctgac actactgatg ctgtccgtga tccacagaca cttgagattc ttgacattac 23280 accatgttct tttggtggtg tcagtgttat aacaccagga acaaatactt ctaatcaggt 23340 tgctgttctt tatcagggtg ttaactgcac agaagtccct gttgctattc atgcagatca 23400 acttactcct acttggcgtg tttattctac aggttctaat gtttttcaaa cacgtgcagg 23460 ctgtttaata ggggctgaat atgtcaacaa ctcatatgag tgtgacatac ccattggtgc 23520 aggtatatgc gctagttatc agactcagac taattctcct cggcgggcac gtagtgtagc 23580 tagtcaatcc atcattgcct acactatgtc acttggtgca gaaaattcag ttgcttactc 23640 taataactct attgccatac ccacaaattt tactattagt gttaccacag aaattctacc 23700 agtgtctatg accaagacat cagtagattg tacaatgtac atttgtggtg attcaactga 23760 atgcagcaat cttttgttgc aatatggcag tttttgtaca caattaaacc gtgctttaac 23820 tggaatagct gttgaacaag acaaaaacac ccaagaagtt tttgcacaag tcaaacaaat 23880 ttacaaaaca ccaccaatta aagattttgg tggttttaat ttttcacaaa tattaccaga 23940 tccatcaaaa ccaagcaaga ggtcatttat tgaagatcta cttttcaaca aagtgacact 24000 tgcagatgct ggcttcatca aacaatatgg tgattgcctt ggtgatattg ctgctagaga 24060 cctcatttgt gcacaaaagt ttaacggcct tactgttttg ccacctttgc tcacagatga 24120 aatgattgct caatacactt ctgcactgtt agcgggtaca atcacttctg gttggacctt 24180 tggtgcaggt gctgcattac aaataccatt tgctatgcaa atggcttata ggtttaatgg 24240 tattggagtt acacagaatg ttctctatga gaaccaaaaa ttgattgcca accaatttaa 24300 tagtgctatt ggcaaaattc aagactcact ttcttccaca gcaagtgcac ttggaaaact 24360 tcaagatgtg gtcaaccaaa atgcacaagc tttaaacacg cttgttaaac aacttagctc 24420 caattttggt gcaatttcaa gtgttttaaa tgatatcctt tcacgtcttg acaaagttga 24480 ggctgaagtg caaattgata ggttgatcac aggcagactt caaagtttgc agacatatgt 24540 gactcaacaa ttaattagag ctgcagaaat cagagcttct gctaatcttg ctgctattaa 24600 aatgtcagag tgtgtacttg gacaatcaaa aagagttgat ttttgtggaa agggctatca 24660 tcttatgtcc ttccctcagt cagcacctca tggtgtagtc ttcttgcatg tgacttatgt 24720 ccctgcacaa gaaaagaact tcacaactgc tcctgccatt tgtcatgatg gaaaagcaca 24780 ctttcctcgt gaaggtgtct ttgtttcaaa tggcacacac tggtttgtaa cacaaaggaa 24840 tttttatgaa ccacaaatca ttactacaga caacacattt gtgtctggta actgtgatgt 24900 tgtaatagga attgtcaaca acacagttta tgatcctttg caacctgaat tagactcatt 24960 caaggaggag ttagataaat attttaagaa tcatacatca ccagatgttg atttaggtga 25020 catctctggc attaatgctt catttgtaaa cattcaaaaa gaaattgacc gcctcaatga 25080 ggttgccaag aatttaaatg aatctctcat cgatctccaa gaacttggaa agtatgagca 25140 gtatataaaa tggccatggt acatttggct aggttttata gctggcttga ttgccatagt 25200 aatggtgaca attatgcttt gctgtatgac cagttgctgt agttgtctca agggctgttg 25260 ttcttgtgga tcctgctgca aatttgatga agacgactct gagccagtgc tcaaaggagt 25320 caaattacat tacacataaa cgaacttatg gatttgttta tgagaatctt cacaattgga 25380 actgtaactt tgaagcaagg tgaaatcaag gatgctactc cttcagattt tgttcgcgct 25440 actgcaacga taccgataca agcctcactc cctttcggat ggcttattgt tggcgttgca 25500 cttcttgctg tttttcagag cgcttccaaa atcataaccc tcaaaaagag atggcaacta 25560 gcactctcca agggtgttca ctttgtttgc aacttgctgt tgttgtttgt aacagtttac 25620 tcacaccttt tgctcgttgc tgctggcctt gaagcccctt ttctctatct ttatgcttta 25680 gtctacttct tgcagagtat aaactttgta agaataataa tgaggctttg gctttgctgg 25740 aaatgccgtt ccaaaaaccc attactttat gatgccaact attttctttg ctggcatact 25800 aattgttacg actattgtat accttacaat agtgtaactt cttcaattgt cattacttca 25860 ggtgatggca caacaagtcc tatttctgaa catgactacc agattggtgg ttatactgaa 25920 aaatgggaat ctggagtaaa agactgtgtt gtattacaca gttacttcac ttcagactat 25980 taccagctgt actcaactca attgagtaca gacactggtg ttgaacatgt taccttcttc 26040 atctacaata aaattgttga tgagcctgaa gaacatgtcc aaattcacac aatcgacggt 26100 tcacccggag ttgttaatcc agtaatggaa ccaatttatg atgaaccgac gacgactact 26160 agcgtgcctt tgtaagcaca agctgatgag tacgaactta tgtactcatt cgtttcggaa 26220 gagacaggta cgttaatagt taatagcgta cttctttttc ttgctttcgt ggtattcttg 26280 ctagttacac tagccatcct tactgcgctt cgattgtgtg cgtactgctg caatattgtt 26340 aacgtgagtc ttgtaaaacc ttctttttac gtttactctc gtgttaaaaa tctgaattct 26400 tctagagttc ctgatcttct ggtctaaacg aactaaatat tatattagtt tttctgtttg 26460 gaactttaat tttagccatg gcagattcca acggtactat taccgttgaa gagcttaaaa 26520 agctccttga acaatggaac ctagtaatag gtttcctatt ccttacatgg atttgtcttc 26580 tacaatttgc ctatgccaac aggaataggt ttttgtatat aattaagtta attttcctct 26640 ggctgttatg gccagtaact ttagcttgtt ttgtgcttgc tgctgtttac agaataaatt 26700 ggatcaccgg tggaattgct atcgcaatgg cttgtcttgt aggcttgatg tggctcagct 26760 acttcattgc ttctttcaga ctgtttgcgc gtacgcgttc catgtggtca ttcaatccag 26820 aaactaacat tcttctcaac gtgccactcc atggcactat tctgaccaga ccgcttctag 26880 aaagtgaact cgtaatcgga gctgtgatcc ttcgtggaca tcttcgtatt gctggacacc 26940 atctaggacg ctgtgacatc aaggacctgc ctaaagaaat cactgttgct acatcacgaa 27000 cgctttctta ttacaaattg ggagcttcgc agcgtgtagc aggtgactca ggttttgctg 27060 catacagtcg ctacaggatt ggcaactata aattaaacac agaccattcc agtagcagtg 27120 acaatattgc tttgcttgta cagtaagtga caacagatgt ttcatctcgt tgactttcag 27180 gttactatag cagagatatt actaattatt atgaggactt ttaaagtttc catttggaat 27240 cttgattaca tcataaacct cataattaaa aatttatcta agtcactaac tgagaataaa 27300 tattctcaat tagatgaaga gcaaccaatg gagattgatt aaacgaacat gaaaattatt 27360 cttttcttgg cactgataac actcgctact tgtgagcttt atcactacca agagtgtgtt 27420 agaggtacaa cagtactttt aaaagaacct tgctcttctg gaacatacga gggcaattca 27480 ccatttcatc ctctagctga taacaaattt gcactgactt gctttagcac tcaatttgct 27540 tttgcttgtc ctgacggcgt aaaacacgtc tatcagttac gtgccagatc agtttcacct 27600 aaactgttca tcagacaaga ggaagttcaa gaactttact ctccaatttt tcttattgtt 27660 gcggcaatag tgtttataac actttgcttc acactcaaaa gaaagacaga atgattgaac 27720 tttcattaat tgacttctat ttgtgctttt tagcctttct gctattcctt gttttaatta 27780 tgcttattat cttttggttc tcacttgaac tgcaagatca taatgaaact tgtcacgcct 27840 aaacgaacat gaaatttctt gttttcttag gaatcatcac aactgtagct gcatttcacc 27900 aagaatgtag tttacagtca tgtactcaac atcaaccata tgtagttgat gacccgtgtc 27960 ctattcactt ctattctaaa tggtatatta gagtaggagc tagaaaatca gcacctttaa 28020 ttgaattgtg cgtggatgag gctggttcta aatcacccat tcagtacatc gatatcggta 28080 attatacagt ttcctgttta ccttttacaa ttaattgcca gaaacctaaa ttgggtagtc 28140 ttgtagtgcg ttgttcgttc tatgaagact ttttagagta tcatgacgtt cgtgttgttt 28200 tagatttcat ctaaacgaac aaacaaacta aaatgtctga taatggaccc caaaatcagc 28260 gaaatgcacc ccgcattacg tttggtggac cctcagattc aactggcagt aaccagaatg 28320 gagaacgcag tggggcgcga tcaaaacaac gtcggcccca aggtttaccc aataatactg 28380 cgtcttggtt caccgctctc actcaacatg gcaaggaaga ccttaaattc cctcgaggac 28440 aaggcgttcc aattaacacc aatagcagtc gagatgacca aattggctac taccgaagag 28500 ctaccagacg aattcgtggt ggtgacggta aaatgaaaga tctcagtcca agatggtatt 28560 tctactacct aggaactggg ccagaagctg gacttcccta tggtgctaac aaagacggca 28620 tcatatgggt tgcaactgag ggagccttga atacaccaaa agatcacatt ggcacccgca 28680 atcctgctaa caatgctgca atcgtgctac aacttcctca aggaacaaca ttgccaaaag 28740 gcttctacgc agaagggagc agaggcggca gtcaagcctc ttctcgttcc tcatcacgta 28800 gtcgcaacag ttcaagaaat tcaactccag gcagctctaa acgaacttct cctgctagaa 28860 tggctggcaa tggcggtgat gctgctcttg ctttgctgct gcttgacaga ttgaaccagc 28920 ttgagagcaa aatgtctggt aaaggccaac aacaacaagg ccaaactgtc actaagaaat 28980 ctgctgctga ggcttctaag aagcctcggc aaaaacgtac tgccactaaa gcatacaatg 29040 taacacaagc tttcggcaga cgtggtccag aacaaaccca aggaaatttt ggggaccagg 29100 aactaatcag acaaggaact gattacaaac attggccgca aattgcacaa tttgccccca 29160 gcgcttcagc gttcttcgga atgtcgcgca ttggcatgga agtcacacct tcgggaacgt 29220 ggttgaccta cacaggtgcc atcaaattgg atgacaaaga tccaaatttc aaagatcaag 29280 tcattttgct gaataagcat attgacgcat acaaaacatt cccaccaaca gagcctaaaa 29340 aggacaaaaa gaagaaggct gatgaaactc aagccttacc gcagagacag aagaaacagc 29400 aaactgtgac tcttcttcct gctgcagatt tggatgattt ctccaaacaa ttgcaacaat 29460 ccatgagcag tgctgactca actcaggcct aaactcatgc agaccacaca aggcagatgg 29520 gctatataaa cgttttcgct tttccgttta cgatatatag tctactcttg tgcagaatga 29580 attctcgtaa ctacatagca caagtagatg tagttaactt taatctcaca tagcaatctt 29640 taatcagtgt gtaacattag ggaggacttg aaagagccac cacattttca ccgaggccac 29700 gcggagtacg atcgagtgta cagtgaacaa tgctagggag agctgcctat atggaagagc 29760 cctaatgtgt aaaattaatt ttagtagtgc taaccccatg tgattttaat agcttctta 29819 <210> 21 <211> 1273 <212> PRT <213> SARS-CoV2 <400> 21 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 1 5 10 15 Asn Phe Thr Asn Arg Thr Gln Leu Pro Ser Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Tyr Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Ser Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 225 230 235 240 Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 245 250 255 Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 260 265 270 Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 275 280 285 Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 290 295 300 Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 305 310 315 320 Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 325 330 335 Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 340 345 350 Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 355 360 365 Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 370 375 380 Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 385 390 395 400 Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 405 410 415 Thr Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 420 425 430 Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 435 440 445 Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 450 455 460 Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 465 470 475 480 Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 485 490 495 Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 500 505 510 Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 515 520 525 Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 530 535 540 Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 545 550 555 560 Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 565 570 575 Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 580 585 590 Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 595 600 605 Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 610 615 620 His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 625 630 635 640 Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu Tyr Val 645 650 655 Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 660 665 670 Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 675 680 685 Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 690 695 700 Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 705 710 715 720 Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 725 730 735 Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 740 745 750 Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 755 760 765 Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 770 775 780 Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 785 790 795 800 Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 805 810 815 Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 820 825 830 Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 835 840 845 Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 850 855 860 Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 865 870 875 880 Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 885 890 895 Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 900 905 910 Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 915 920 925 Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 930 935 940 Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 945 950 955 960 Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 965 970 975 Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 980 985 990 Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 995 1000 1005 Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 1010 1015 1020 Ala Ala Ile Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 1025 1030 1035 1040 Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 1045 1050 1055 Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 1060 1065 1070 Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 1075 1080 1085 Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 1090 1095 1100 Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 1105 1110 1115 1120 Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 1125 1130 1135 Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 1140 1145 1150 Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 1155 1160 1165 Ile Ser Gly Ile Asn Ala Ser Phe Val Asn Ile Gln Lys Glu Ile Asp 1170 1175 1180 Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 1185 1190 1195 1200 Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 1205 1210 1215 Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 1220 1225 1230 Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 1235 1240 1245 Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 1250 1255 1260 Leu Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 22 <211> 29883 <212> DNA <213> SARS-CoV2 <220> <221> misc_feature <222> (29849)..(29883) <223> n is a, c, g, or t <400> 22 attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 60 gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120 cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 180 ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240 tgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 300 acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 360 agactccgtg gaggaggtct tatcagaggc acgtcaacat cttaaagatg gcacttgtgg 420 cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 480 acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 540 cgaaggcatt cagtacggtc gtagtggtga gacacttggt gtccttgtcc ctcatgtggg 600 cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 660 tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 720 tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 780 actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 840 ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 900 atgcactttg tctgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 960 tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1020 gacacctttt gaaattaaat tggcaaagaa atttgacacc ttcaatgggg aatgtccaaa 1080 ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1140 gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1200 caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1260 gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1320 aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1380 atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1440 cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc 1500 ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1560 ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 1620 aatactccaa aaagagaaag tcaacatcaa tattgttggt gactttaaac ttaatgaaga 1680 gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 1740 aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac 1800 aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1860 tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 1920 tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1980 aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 2040 taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg 2100 gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2160 agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 2220 ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcaa aggaaattaa 2280 ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 2340 tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca 2400 ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2460 tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 2520 aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2580 agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 2640 aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa caaacaatac 2700 cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 2760 agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2820 acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 2880 ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc 2940 actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3000 tgagtttaaa ttggcttcac atatgtattg ttctttttac cctccagatg aggatgaaga 3060 agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3120 agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 3180 agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3240 cggcagtgag gacaatcaga caactattat tcaaacaatt gttgaggttc aacctcaatt 3300 agagatggaa cttacaccag ttgttcagac tattgaagtg aatagtttta gtggttattt 3360 aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 3420 aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc 3480 aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3540 tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 3600 acactgtctt catgttgtcg gcccaaatgt taacaaaggt gaagacattc aacttcttaa 3660 gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 3720 tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa 3780 tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3840 aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa 3900 gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3960 caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 4020 cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4080 tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttgttca 4140 agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat 4200 gctagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4260 gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc 4320 cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4380 ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg 4440 tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4500 agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 4560 gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4620 tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 4680 agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc 4740 ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4800 agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga 4860 taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4920 ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4980 aacagtagac aacattaatc tccacacgca agttgtggac atgtcaatga catatggaca 5040 acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 5100 acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5160 tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5220 cactaaaaag tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa 5280 caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5340 acctgctcta caagatgctt attacagagc aagggctggt gaagctgata acttttgtgc 5400 acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5460 gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 5520 taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5580 cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 5640 agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5700 tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 5760 gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt 5820 acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5880 ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 5940 tgaccctaag ttggacaatt attataagaa agacaattct tattttacag agcaaccaat 6000 tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 6060 tgataatatc aaatttgctg atgatttaaa ccagttaact ggttataaga aacctgcttc 6120 aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6180 taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 6240 gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6300 tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 6360 cgcgcaggga atggataatc ttgcctgcga agatctaaaa ccagtctctg aagaagtagt 6420 ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 6480 aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6540 cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 6600 attatctaga gtattaggtt tgaaaaccct tgttactcat ggtttagctg ctgttaatag 6660 tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6720 aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 6780 ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc 6840 atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6900 ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttacaatttg 6960 gtttttacta ttaagtgttt gcctaggttc tttaatctac tcaaccgctg ctttaggtgt 7020 tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7080 ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7140 tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7200 atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat 7260 tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7320 ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 7380 acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7440 tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 7500 ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag 7560 gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7620 tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 7680 cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7740 tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7800 ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7860 taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7920 atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 7980 agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8040 tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 8100 agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac 8160 ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8220 tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 8280 ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8340 tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8400 atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc 8460 tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8520 tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 8580 gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8640 tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8700 tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc 8760 tgattttgac acatggttta gccagcgtgg tggtagttat actaatgaca aagcttgccc 8820 attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 8880 gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8940 tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9000 ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata 9060 ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9120 acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 9180 tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9240 agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 9300 atctttacca ggagttttct gtggtgtaga tgctgtaaat ttacttacta atatgtttac 9360 accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9420 tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 9480 tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9540 ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9600 gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9660 cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9720 tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 9780 tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa 9840 gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9900 taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 9960 tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10020 accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10080 atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg 10140 tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10200 gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 10260 ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10320 taaggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 10380 acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc 10440 tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10500 ttttaacata gattatgact gtgtctcttt ttgttacatg caccatatgg aattaccaac 10560 tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10620 aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 10680 cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga 10740 ctttaacctt gtggctatga agtacaatta tgaacctcta acacaagacc atgttgacat 10800 actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 10860 agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10920 tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 10980 gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt 11040 agttttagtc cagagtactc aatggtcttt gttctttttt ttgtatgaaa atgccttttt 11100 accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 11160 gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11220 ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11280 tagtttgaag ctaaaagact gtgttatgta tgcatcagct gtagtgttac taatccttat 11340 gacagcaaga actgtgtatg atgatggtgc taggagagtg tggacactta tgaatgtctt 11400 gacactcgtt tataaagttt attatggtaa tgctttagat caagccattt ccatgtgggc 11460 tcttataatc tctgttactt ctaactactc aggtgtagtt acaactgtca tgtttttggc 11520 cagaggtatt gtttttatgt gtgttgagta ttgccctatt ttcttcataa ctggtaatac 11580 acttcagtgt ataatgctag tttattgttt cttaggctat ttttgtactt gttactttgg 11640 cctcttttgt ttactcaacc gctactttag actgactctt ggtgtttatg attacttagt 11700 ttctacacag gagtttagat atatgaattc acagggacta ctcccaccca agaatagcat 11760 agatgccttc aaactcaaca ttaaattgtt gggtgttggt ggcaaacctt gtatcaaagt 11820 agccactgta cagtctaaaa tgtcagatgt aaagtgcaca tcartagtct tactctcagt 11880 tttgcaacaa ctcagagtag aatcatcatc taaattgtgg gctcaatgtg tccagttaca 11940 caatgacatt ctcttagcta aagatactac tgaagccttt gaaaaaatgg tttcactact 12000 ttctgttttg ctttccatgc agggtgctgt agacataaac aagctttgtg aagaaatgct 12060 ggacaacagg gcaaccttac aagctatagc ctcagagttt agttcccttc catcatatgc 12120 agcttttgct actgctcaag aagcttatga gcaggctgtt gctaatggtg attctgaagt 12180 tgttcttaaa aagttgaaga agtctttgaa tgtggctaaa tctgaatttg accgtgatgc 12240 agccatgcaa cgtaagttgg aaaagatggc tgatcaagct atgacccaaa tgtataaaca 12300 ggctagatct gaggacaaga gggcaaaagt tactagtgct atgcagacaa tgcttttcac 12360 tatgcttaga aagttggata atgatgcact caacaacatt atcaacaatg caagagatgg 12420 ttgtgttccc ttgaacataa tacctcttac aacagcagcc aaactaatgg ttgtcatacc 12480 agactataac acatataaaa atacgtgtga tggtacaaca tttacttatg catcagcatt 12540 gtgggaaatc caacaggttg tagatgcaga tagtaaaatt gttcaactta gtgaaattag 12600 tatggacaat tcacctaatt tagcatggcc tcttattgta acagctttaa gggccaattc 12660 tgctgtcaaa ttacagaata atgagcttag tcctgttgca ctacgacaga tgtcttgtgc 12720 tgccggtact acacaaactg cttgcactga tgacaatgcg ttagcttact acaacacaac 12780 aaagggaggt aggtttgtac ttgcactgtt atccgattta caggatttga aatgggctag 12840 attccctaag agtgatggaa ctggtactat ctatacagaa ctggaaccac cttgtaggtt 12900 tgttacagac acacctaaag gtcctaaagt gaagtattta tactttatta aaggattaaa 12960 caacctaaat agaggtatgg tacttggtag tttagctgcc acagtacgtc tacaagctgg 13020 taatgcaaca gaagtgcctg ccaattcaac tgtattatct ttctgtgctt ttgctgtaga 13080 tgctgctaaa gcttacaaag attatctagc tagtggggga caaccaatca ctaattgtgt 13140 taagatgttg tgtacacaca ctggtactgg tcaggcaata acagttacac cggaagccaa 13200 tatggatcaa gaatcctttg gtggtgcatc gtgttgtctg tactgccgtt gccacataga 13260 tcatccaaat cctaaaggat tttgtgactt aaaaggtaag tatgtacaaa tacctacaac 13320 ttgtgctaat gaccctgtgg gttttacact taaaaacaca gtctgtaccg tctgcggtat 13380 gtggaaaggt tatggctgta gttgtgatca actccgcgaa cccatgcttc agtcagctga 13440 tgcacaatcg tttttaaacg ggtttgcggt gtaagtgcag cccgtcttac accgtgcggc 13500 acaggcacta gtactgatgt cgtatacagg gcttttgaca tctacaatga taaagtagct 13560 ggttttgcta aattcctaaa aactaattgt tgtcgcttcc aagaaaagga cgaagatgac 13620 aatttaattg attcttactt tgtagttaag agacacactt tctctaacta ccaacatgaa 13680 gaaacaattt ataatttact taaggattgt ccagctgttg ctaaacatga cttctttaag 13740 tttagaatag acggtgacat ggtaccacat atatcacgtc aacgtcttac taaatacaca 13800 atggcagacc tcgtctatgc tttaaggcat tttgatgaag gtaattgtga cacattaaaa 13860 gaaatacttg tcacatacaa ttgttgtgat gatgattatt tcaataaaaa ggactggtat 13920 gattttgtag aaaacccaga tatattacgc gtatacgcca acttaggtga acgtgtacgc 13980 caagctttgt taaaaacagt acaattctgt gatgccatgc gaaatgctgg tattgttggt 14040 gtactgacat tagataatca agatctcaat ggtaactggt atgatttcgg tgatttcata 14100 caaaccacgc caggtagtgg agttcctgtt gtagattctt attattcatt gttaatgcct 14160 atattaacct tgaccagggc tttaactgca gagtcacatg ttgacactga cttaacaaag 14220 ccttacatta agtgggattt gttaaaatat gacttcacgg aagagaggtt aaaactcttt 14280 gaccgttatt ttaaatattg ggatcagaca taccacccaa attgtgttaa ctgtttggat 14340 gacagatgca ttctgcattg tgcaaacttt aatgttttat tctctacagt gttcccactt 14400 acaagttttg gaccactagt gagaaaaata tttgttgatg gtgttccatt tgtagtttca 14460 actggatacc acttcagaga gctaggtgtt gtacataatc aggatgtaaa cttacatagc 14520 tctagactta gttttaagga attacttgtg tatgctgctg accctgctat gcacgctgct 14580 tctggtaatc tattactaga taaacgcact acgtgctttt cagtagctgc acttactaac 14640 aatgttgctt ttcaaactgt caaacctggt aattttaaca aagacttcta tgactttgct 14700 gtgtctaagg gtttctttaa ggaaggaagt tctgttgaat taaaacactt cttctttgct 14760 caggatggta atgctgctat cagcgattat gactactatc gttataatct accaacaatg 14820 tgtgatatca gacaactact atttgtagtt gaagttgttg ataagtactt tgattgttac 14880 gatggtggct gtattaatgc taaccaagtc atcgtcaaca acctagacaa atcagctggt 14940 tttccattta ataaatgggg taaggctaga ctttattatg attcaatgag ttatgaggat 15000 caagatgcac ttttcgcata tacaaaacgt aatgtcatcc ctactataac tcaaatgaat 15060 cttaagtatg ccattagtgc aaagaataga gctcgcaccg tagctggtgt ctctatctgt 15120 agtactatga ccaatagaca gtttcatcaa aaattattga aatcaatagc cgccactaga 15180 ggagctactg tagtaattgg aacaagcaaa ttctatggtg gttggcacaa catgttaaaa 15240 actgtttata gtgatgtaga aaaccctcat cttatgggtt gggattatcc taaatgtgat 15300 agagccatgc ctaacatgct tagaattatg gcctcacttg ttcttgctcg caaacataca 15360 acgtgttgta gcttgtcaca ccgtttctat agattagcta atgagtgtgc tcaagtattg 15420 agtgaaatgg tcatgtgtgg cggttcacta tatgttaaac caggtggaac ctcatcagga 15480 gatgccacaa ctgcttatgc taatagtgtt tttaacattt gtcaagctgt cacggccaat 15540 gttaatgcac ttttatctac tgatggtaac aaaattgccg ataagtatgt ccgcaattta 15600 caacacagac tttatgagtg tctctataga aatagagatg ttgacacaga ctttgtgaat 15660 gagttttacg catatttgcg taaacatttc tcaatgatga tactctctga cgatgctgtt 15720 gtgtgtttca atagcactta tgcatctcaa ggtctagtgg ctagcataaa gaactttaag 15780 tcagttcttt attatcaaaa caatgttttt atgtctgaag caaaatgttg gactgagact 15840 gaccttacta aaggacctca tgaattttgc tctcaacata caatgctagt taaacagggt 15900 gatgattatg tgtaccttcc ttacccagat ccatcaagaa tcctaggggc cggctgtttt 15960 gtagatgata tcgtaaaaac agatggtaca cttatgattg aacggttcgt gtctttagct 16020 atagatgctt acccacttac taaacatcct aatcaggagt atgctgatgt ctttcatttg 16080 tacttacaat acataagaaa gctacatgat gagttaacag gacacatgtt agacatgtat 16140 tctgttatgc ttactaatga taacacctca aggtattggg aacctgagtt ttatgaggct 16200 atgtacacac cgcatacagt cttacaggct gttggggctt gtgttctttg caattcacag 16260 acttcattaa gatgtggtgc ttgcatacgt agaccattct tatgttgtaa atgctgttac 16320 gaccatgtca tatcaacatc acataaatta gtcttgtctg ttaatccgta tgtttgcaat 16380 gctccaggtt gtgatgtcac agatgtgact caactttact taggaggtat gagctattat 16440 tgtaaatcac ataaaccacc cattagtttt ccattgtgtg ctaatggaca agtttttggt 16500 ttatataaaa atacatgtgt tggtagcgat aatgttactg actttaatgc aattgcaaca 16560 tgtgactgga caaatgctgg tgattacatt ttagctaaca cctgtactga aagactcaag 16620 ctttttgcag cagaaacgct caaagctact gaggagacat ttaaactgtc ttatggtatt 16680 gctactgtac gtgaagtgct gtctgacaga gaattacatc tttcatggga agttggtaaa 16740 cctagaccac cacttaaccg aaattatgtc tttactggtt atcgtgtaac taaaaacagt 16800 aaagtacaaa taggagagta cacctttgaa aaaggtgact atggtgatgc tgttgtttac 16860 cgaggtacaa caacttacaa attaaatgtt ggtgattatt ttgtgctgac atcacataca 16920 gtaatgccat taagtgcacc tacactagtg ccacaagagc actatgttag aattactggc 16980 ttatacccaa cactcaatat ctcagatgag ttttctagca atgttgcaaa ttatcaaaag 17040 gttggtatgc aaaagtattc tacactccag ggaccacctg gtactggtaa gagtcatttt 17100 gctattggcc tagctctcta ctacccttct gctcgcatag tgtatacagc ttgctctcat 17160 gccgctgttg atgcactatg tgagaaggca ttaaaatatt tgcctataga taaatgtagt 17220 agaattatac ctgcacgtgc tcgtgtagag tgttttgata aattcaaagt gaattcaaca 17280 ttagaacagt atgtcttttg tactgtaaat gcattgcctg agacgacagc agatatagtt 17340 gtctttgatg aaatttcaat ggccacaaat tatgatttga gtgttgtcaa tgccagatta 17400 cgtgctaagc actatgtgta cattggcgac cctgctcaat tacctgcacc acgcacattg 17460 ctaactaagg gcacactaga accagaatat ttcaattcag tgtgtagact tatgaaaact 17520 ataggtccag acatgttcct cggaacttgt cggcgttgtc ctgctgaaat tgttgacact 17580 gtgagtgctt tggtttatga taataggctt aaagcacata aagacaaatc agctcaatgc 17640 tttaaaatgt tttataaggg tgttatcacg catgatgttt catctgcaat taacaggcca 17700 caaataggcg tggtaagaga attccttaca cgtaaccctg cttggagaaa agctgtcttt 17760 atttcacctt ataattcaca gaatgctgta gcctcaaaga ttttgggact accaactcaa 17820 actgttgatt catcacaggg ctcagaatat gactatgtca tattcactca aaccactgaa 17880 acagctcact cttgtaatgt aaacagattt aatgttgcta ttaccagagc aaaagtaggc 17940 atactttgca taatgtctga tagagacctt tatgacaagt tgcaatttac aagtcttgaa 18000 attccacgta ggaatgtggc aactttacaa gctgaaaatg taacaggact ctttaaagat 18060 tgtagtaagg taatcactgg gttacatcct acacaggcac ctacacacct cagtgttgac 18120 actaaattca aaactgaagg tttatgtgtt gacatacctg gcatacctaa ggacatgacc 18180 tatagaagac tcatctctat gatgggtttt aaaatgaatt atcaagttaa tggttaccct 18240 aacatgttta tcacccgcga agaagctata agacatgtac gtgcatggat tggcttcgat 18300 gtcgaggggt gtcatgctac tagagaagct gttggtacca atttaccttt acagctaggt 18360 ttttctacag gtgttaacct agttgctgta cctacaggtt atgttgatac acctaataat 18420 acagattttt ccagagttag tgctaaacca ccgcctggag atcaatttaa acacctcata 18480 ccacttatgt acaaaggact tccttggaat gtagtgcgta taaagattgt acaaatgtta 18540 agtgacacac ttaaaaatct ctctgacaga gtcgtatttg tcttatgggc acatggcttt 18600 gagttgacat ctatgaagta ttttgtgaaa ataggacctg agcgcacctg ttgtctatgt 18660 gatagacgtg ccacatgctt ttccactgct tcagacactt atgcctgttg gcatcattct 18720 attggatttg attacgtcta taatccgttt atgattgatg ttcaacaatg gggttttaca 18780 ggtaacctac aaagcaacca tgatctgtat tgtcaagtcc atggtaatgc acatgtagct 18840 agttgtgatg caatcatgac taggtgtcta gctgtccacg agtgctttgt taagcgtgtt 18900 gactggacta ttgaatatcc tataattggt gatgaactga agattaatgc ggcttgtaga 18960 aaggttcaac acatggttgt taaagctgca ttattagcag acaaattccc agttcttcac 19020 gacattggta accctaaagc tattaagtgt gtacctcaag ctgatgtagg atggaagttc 19080 tatgatgcac agccttgtag tgacaaagct tataaaatag aagaattatt ctattcttat 19140 gccacacatt ctgacaaatt cacagatggt gtatgcctat tttggaattg caatgtcgat 19200 agatatcctg ctaattccat tgtttgtaga tttgacacta gagtgctatc taaccttaac 19260 ttgcctggtt gtgatggtgg cagtttgtat gtaaataaac atgcattcca cacaccagct 19320 tttgataaaa gtgcttttgt taatttaaaa caattaccat ttttctatta ctctgacagt 19380 ccatgtgagt ctcatggaaa acaagtagtg tcagatatag attatgtacc actaaagtct 19440 gctacgtgta taacacgttg caatttaggt ggtgctgtct gtagacatca tgctaatgag 19500 tacagattgt atctcgatgc ttataacatg atgatctcag ctggctttag cttgtgggtt 19560 tacaaacaat ttgatactta taacctctgg aacactttta caagacttca gagtttagaa 19620 aatgtggctt ttaatgttgt aaataaggga cactttgatg gacaacaggg tgaagtacca 19680 gtttctatca ttaataacac tgtttacaca aaagttgatg gtgttgatgt agaattgttt 19740 gaaaataaaa caacattacc tgttaatgta gcatttgagc tttgggctaa gcgcaacatt 19800 aaaccagtac cagaggtgaa aatactcaat aatttgggtg tggatattgc tgctaatact 19860 gtgatctggg actacaaaag agatgctcca gcacatatat ctactattgg tgtttgttct 19920 atgactgaca tagccaagaa accaactgaa acgatttgtg caccactcac tgtctttttt 19980 gatggtagag ttgatggtca agtagactta tttagaaatg cccgtaatgg tgttcttatt 20040 acagaaggta gtgttaaagg tttacaacca tctgtaggtc ccaaacaagc tagtcttaat 20100 ggagtcacat taattggaga agccgtaaaa acacagttca attattataa gaaagttgat 20160 ggtgttgtcc aacaattacc tgaaacttac tttactcaga gtagaaattt acaagaattt 20220 aaacccagga gtcaaatgga aattgatttc ttagaattag ctatggatga attcattgaa 20280 cggtataaat tagaaggcta tgccttcgaa catatcgttt atggagattt tagtcatagt 20340 cagttaggtg gtttacatct actgattgga ctagctaaac gttttaagga atcacctttt 20400 gaattagaag attttattcc tatggacagt acagttaaaa actatttcat aacagatgcg 20460 caaacaggtt catctaagtg tgtgtgttct gttattgatt tattacttga tgattttgtt 20520 gaaataataa aatcccaaga tttatctgta gtttctaagg ttgtcaaagt gactattgac 20580 tatacagaaa tttcatttat gctttgtgta aagatggcca tgtagaaaca ttttacccaa 20640 aattacaatc tagtcaagcg tggcaaccgg gtgttgctat gcctaatctt tacaaaatgc 20700 aaagaatgct attagaaaag tgtgaccttc aaaattatgg tgatagtgca acattaccta 20760 aaggcataat gatgaatgtc gcaaaatata ctcaactgtg tcaatattta aacacattaa 20820 cattagctgt accctataat atgagagtta tacattttgg tgctggttct gataaaggag 20880 ttgcaccagg tacagctgtt ttaagacagt ggttgcctac gggtacgctg cttgtcgatt 20940 cagatcttaa tgactttgtc tctgatgcag attcaacttt gattggtgat tgtgcaactg 21000 tacatacagc taataaatgg gatctcatta ttagtgatat gtacgaccct aagactaaaa 21060 atgttacaaa agaaaatgac tctaaagagg gttttttcac ttacatttgt gggtttatac 21120 aacaaaagct agctcttgga ggttccgtgg ctataaagat aacagaacat tcttggaatg 21180 ctgatcttta taagctcatg ggacacttcg catggtggac agcctttgtt actaatgtga 21240 atgcgtcatc atctgaagca tttttaattg gatgtaatta tcttggcaaa ccacgcgaac 21300 aaatagatgg ttatgtcatg catgcaaatt acatattttg gaggaataca aatccaattc 21360 agttgtcttc ctattcttta tttgacatga gtaaatttcc ccttaaatta aggggtactg 21420 ctgttatgtc tttaaaagaa ggtcaaatca atgatatgat tttatctctt cttagtaaag 21480 gtagacttat aattagagaa aacaacagag ttgttatttc tagtgatgtt cttgttaaca 21540 actaaacgaa caatgtttgt ttttcttgtt ttattgccac tagtctctag tcagtgtgtt 21600 aatcttacaa ccagaactca attaccccct gcatacacta attctttcac acgtggtgtt 21660 tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga cttgttctta 21720 cctttctttt ccaatgttac ttggttccat gctatctctg ggaccaatgg tactaagagg 21780 tttgataacc ctgtcctacc atttaatgat ggtgtttatt ttgcttccac tgagaagtct 21840 aacataataa gaggctggat ttttggtact actttagatt cgaagaccca gtccctactt 21900 attgttaata acgctactaa tgttgttatt aaagtctgtg aatttcaatt ttgtaatgat 21960 ccatttttgg gtgtttacca caaaaacaac aaaagttgga tggaaagtga gttcagagtt 22020 tattctagtg cgaataattg cacttttgaa tatgtctctc agccttttct tatggacctt 22080 gaaggaaaac agggtaattt caaaaatctt agggaatttg tgtttaagaa tattgatggt 22140 tattttaaaa tatattctaa gcacacgcct attaatttag tgcgtgatct ccctcagggt 22200 ttttcggctt tagaaccatt ggtagatttg ccaataggta ttaacatcac taggtttcaa 22260 actttacttg ctttacatag aagttatttg actcctggtg attcttcttc aggttggaca 22320 gctggtgctg cagcttatta tgtgggttat cttcaaccta ggacttttct attaaaatat 22380 aatgaaaatg gaaccattac agatgctgta gactgtgcac ttgaccctct ctcagaaaca 22440 aagtgtacgt tgaaatcctt cactgtagaa aaaggaatct atcaaacttc taactttaga 22500 gtccaaccaa cagaatctat tgttagattt cctaatatta caaacttgtg cccttttggt 22560 gaagttttta acgccaccag atttgcatct gtttatgctt ggaacaggaa gagaatcagc 22620 aactgtgttg ctgattattc tgtcctatat aattccgcat cattttccac ttttaagtgt 22680 tatggagtgt ctcctactaa attaaatgat ctctgcttta ctaatgtcta tgcagattca 22740 tttgtaatta gaggtgatga agtcagacaa atcgctccag ggcaaactgg aaagattgct 22800 gattataatt ataaattacc agatgatttt acaggctgcg ttatagcttg gaattctaac 22860 aatcttgatt ctaaggttgg tggtaattat aattacctgt atagattgtt taggaagtct 22920 aatctcaaac cttttgagag agatatttca actgaaatct atcaggccgg tagcacacct 22980 tgtaatggtg ttgaaggttt taattgttac tttcctttac aatcatatgg tttccaaccc 23040 acttatggtg ttggttacca accatacaga gtagtagtac tttcttttga acttctacat 23100 gcaccagcaa ctgtttgtgg acctaaaaag tctactaatt tggttaaaaa caaatgtgtc 23160 aatttcaact tcaatggttt aacaggcaca ggtgttctta ctgagtctaa caaaaagttt 23220 ctgcctttcc aacaatttgg cagagacatt gatgacacta ctgatgctgt ccgtgatcca 23280 cagacacttg agattcttga cattacacca tgttcttttg gtggtgtcag tgttataaca 23340 ccaggaacaa atacttctaa ccaggttgct gttctttatc agggtgttaa ctgcacagaa 23400 gtccctgttg ctattcatgc agatcaactt actcctactt ggcgtgttta ttctacaggt 23460 tctaatgttt ttcaaacacg tgcaggctgt ttaatagggg ctgaacatgt caacaactca 23520 tatgagtgtg acatacccat tggtgcaggt atatgcgcta gttatcagac tcagactaat 23580 tctcatcggc gggcacgtag tgtagctagt caatccatca ttgcctacac tatgtcactt 23640 ggtgcagaaa attcagttgc ttactctaat aactctattg ccatacccat aaattttact 23700 attagtgtta ccacagaaat tctaccagtg tctatgacca agacatcagt agattgtaca 23760 atgtacattt gtggtgattc aactgaatgc agcaatcttt tgttgcaata tggcagtttt 23820 tgtacacaat taaaccgtgc tttaactgga atagctgttg aacaagacaa aaacacccaa 23880 gaagtttttg cacaagtcaa acaaatttac aaaacaccac caattaaaga ttttggtggt 23940 tttaattttt cacaaatatt accagatcca tcaaaaccaa gcaagaggtc atttattgaa 24000 gatctacttt tcaacaaagt gacacttgca gatgctggct tcatcaaaca atatggtgat 24060 tgccttggtg atattgctgc tagagacctc atttgtgcac aaaagtttaa cggccttact 24120 gttttgccac ctttgctcac agatgaaatg attgctcaat acacttctgc actgttagcg 24180 ggtacaatca cttctggttg gacctttggt gcaggtgctg cattacaaat accatttgct 24240 atgcaaatgg cttataggtt taatggtatt ggagttacac agaatgttct ctatgagaac 24300 caaaaattga ttgccaacca atttaatagt gctattggca aaattcaaga ctcactttct 24360 tccacagcaa gtgcacttgg aaaacttcaa gatgtggtca accaaaatgc acaagcttta 24420 aacacgcttg ttaaacaact tagctccaat tttggtgcaa tttcaagtgt tttaaatgat 24480 atccttgcac gtcttgacaa agttgaggct gaagtgcaaa ttgataggtt gatcacaggc 24540 agacttcaaa gtttgcagac atatgtgact caacaattaa ttagagctgc agaaatcaga 24600 gcttctgcta atcttgctgc tactaaaatg tcagagtgtg tacttggaca atcaaaaaga 24660 gttgattttt gtggaaaggg ctatcatctt atgtccttcc ctcagtcagc acctcatggt 24720 gtagtcttct tgcatgtgac ttatgtccct gcacaagaaa agaacttcac aactgctcct 24780 gccatttgtc atgatggaaa agcacacttt cctcgtgaag gtgtctttgt ttcaaatggc 24840 acacactggt ttgtaacaca aaggaatttt tatgaaccac aaatcattac tacacacaac 24900 acatttgtgt ctggtaactg tgatgttgta ataggaattg tcaacaacac agtttatgat 24960 cctttgcaac ctgaattaga ctcattcaag gaggagttag ataaatattt taagaatcat 25020 acatcaccag atgttgattt aggtgacatc tctggcatta atgcttcagt tgtaaacatt 25080 caaaaagaaa ttgaccgcct caatgaggtt gccaagaatt taaatgaatc tctcatcgat 25140 ctccaagaac ttggaaagta tgagcagtat ataaaatggc catggtacat ttggctaggt 25200 tttatagctg gcttgattgc catagtaatg gtgacaatta tgctttgctg tatgaccagt 25260 tgctgtagtt gtctcaaggg ctgttgttct tgtggatcct gctgcaaatt tgatgaagac 25320 gactctgagc cagtgctcaa aggagtcaaa ttacattaca cataaacgaa cttatggatt 25380 tgtttatgag aatcttcaca attggaactg taactttgaa gcaaggtgaa atcaaggatg 25440 ctactccttc agattttgtt cgcgctactg caacgatacc gatacaagcc tcactccctt 25500 tcggatggct tattgttggc gttgcacttc ttgctgtttt tcagagcgct tccaaaatca 25560 taaccctcaa aaagagatgg caactagcac tctccaaggg tgttcacttt gtttgcaact 25620 tgctgttgtt gtttgtaaca gtttactcac accttttgct cgttgctgct ggccttgaag 25680 ccccttttct ctatctttat gctttagtct acttcttgca gagtataaac tttgtaagaa 25740 taataatgag gctttggctt tgctggaaat gccgttccaa aaacccatta ctttatgatg 25800 ccaactattt tctttgctgg catactaatt gttacgacta ttgtatacct tacaatagtg 25860 taacttcttc aattgtcatt acttcaggtg atggcacaac aagtcctatt tctgaacatg 25920 actaccagat tggtggttat actgaaaaat gggaatctgg agtaaaagac tgtgttgtat 25980 tacacagtta cttcacttca gactattacc agctgtactc aactcaattg agtacagaca 26040 ctggtgttga acatgttacc ttcttcatct acaataaaat tgttgatgag cctgaagaac 26100 atgtccaaat tcacacaatc gacggttcat ccggagttgt taatccagta atggaaccaa 26160 tttatgatga accgacgacg actactagcg tgcctttgta agcacaagct gatgagtacg 26220 aacttatgta ctcattcgtt tcggaagaga caggtacgtt aatagttaat agcgtacttc 26280 tttttcttgc tttcgtggta ttcttgctag ttacactagc catccttact gcgcttcgat 26340 tgtgtgcgta ctgctgcaat attgttaacg tgagtcttgt aaaaccttct ttttacgttt 26400 actctcgtgt taaaaatctg aattcttcta gagttcctga tcttctggtc taaacgaact 26460 aaatattata ttagtttttc tgtttggaac tttaatttta gccatggcag attccaacgg 26520 tactattacc gttgaagagc ttaaaaagct ccttgaacaa tggaacctag taataggttt 26580 cctattcctt acatggattt gtcttctaca atttgcctat gccaacagga ataggttttt 26640 gtatataatt aagttaattt tcctctggct gttatggcca gtaactttag cttgttttgt 26700 gcttgctgct gtttacagaa taaattggat caccggtgga attgctatcg caatggcttg 26760 tcttgtaggc ttgatgtggc tcagctactt cattgcttct ttcagactgt ttgcgcgtac 26820 gcgttccatg tggtcattca atccagaaac taacattctt ctcaacgtgc cactccatgg 26880 cactattctg accagaccgc ttctagaaag tgaactcgta atcggagctg tgatccttcg 26940 tggacatctt cgtattgctg gacaccatct aggacgctgt gacatcaagg acctgcctaa 27000 agaaatcact gttgctacat cacgaacgct ttcttattac aaattgggag cttcgcagcg 27060 tgtagcaggt gactcaggtt ttgctgcata cagtcgctac aggattggca actataaatt 27120 aaacacagac cattccagta gcagtgacaa tattgctttg cttgtacagt aagtgacaac 27180 agatgtttca tctcgttgac tttcaggtta ctatagcaga gatattacta attattatga 27240 ggacttttaa agtttccatt tggaatcttg attacatcat aaacctcata attaaaaatt 27300 tatctaagtc actaactgag aataaatatt ctcaattaga tgaagagcaa ccaatggaga 27360 ttgattaaac gaacatgaaa attattcttt tcttggcact gataacactc gctacttgtg 27420 agctttatca ctaccaagag tgtgttagag gtacaacagt acttttaaaa gaaccttgct 27480 cttctggaac atacgagggc aattcaccat ttcatcctct agctgataac aaatttgcac 27540 tgacttgctt tagcactcaa tttgcttttg cttgtcctga cggcgtaaaa cacgtctatc 27600 agttacgtgc cagatcagtt tcacctaaac tgttcatcag acaagaggaa gttcaagaac 27660 tttactctcc aatttttctt attgttgcgg caatagtgtt tataacactt tgcttcacac 27720 tcaaaagaaa gacagaatga ttgaactttc attaattgac ttctatttgt gctttttagc 27780 ctttctgcta ttccttgttt taattatgct tattatcttt tggttctcac ttgaactgca 27840 agatcataat gaaacttgtc acgcctaaac gaacatgaaa tttcttgttt tcttaggaat 27900 catcacaact gtagctgcat ttcaccaaga atgtagttta cagtcatgta cttaacatca 27960 accatatgta gttgatgacc cgtgtcctat tcacttctat tctaaatggt atattagagt 28020 aggagctata aaatcagcac ctttaattga attgtgcgtg gatgaggctg gttctaaatc 28080 acccattcag tgcatcgata tcggtaatta tacagtttcc tgtttacctt ttacaattaa 28140 ttgccaggaa cctaaattgg gtagtcttgt agtgcgttgt tcgttctatg aagacttttt 28200 agagtatcat gacgttcgtg ttgttttaga tttcatctaa acgaacaaac taaatgtctc 28260 taaatggacc ccaaaatcag cgaaatgcac cccgcattac gtttggtgga ccctcagatt 28320 caactggcag taaccagaat ggagaacgca gtggggcgcg atcaaaacaa cgtcggcccc 28380 aaggtttacc caataatact gcgtcttggt tcaccgctct cactcaacat ggcaaggaag 28440 accttaaatt ccctcgagga caaggcgttc caattaacac caatagcagt ccagatgacc 28500 aaattggcta ctaccgaaga gctaccagac gaattcgtgg tggtgacggt aaaatgaaag 28560 atctcagtcc aagatggtat ttctactacc taggaactgg gccagaagct ggacttccct 28620 atggtgctaa caaagacggc atcatatggg ttgcaactga gggagccttg aatacaccaa 28680 aagatcacat tggcacccgc aatcctgcta acaatgctgc aatcgtgcta caacttcctc 28740 aaggaacaac attgccaaaa ggcttctacg cagaagggag cagaggcggc agtcaagcct 28800 cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa ttcaactcca ggcagcagta 28860 aacgaacttc tcctgctaga atggctggca atggcggtga tgctgctctt gctttgctgc 28920 tgcttgacag attgaaccag cttgagagca aaatgtttgg taaaggccaa caacaacaag 28980 gccaaactgt cactaagaaa tctgctgctg aggcttctaa gaagcctcgg caaaaacgta 29040 ctgccactaa agcatacaat gtaacacaag ctttcggcag acgtggtcca gaacaaaccc 29100 aaggaaattt tggggaccag gaactaatca gacaaggaac tgattacaaa cattggccgc 29160 aaattgcaca atttgccccc agcgcttcag cgttcttcgg aatgtcgcgc attggcatgg 29220 aagtcacacc ttcgggaacg tggttgacct acacaggtgc catcaaattg gatgacaaag 29280 atccaaattt caaagatcaa gtcattttgc tgaataagca tattgacgca tacaaaacat 29340 tcccaccaac agagcctaaa aaggacaaaa agaagaaggc tgatgaaact caagccttac 29400 cgcagagaca gaagaaacag caaactgtga ctcttcttcc tgctgcagat ttggatgatt 29460 tctccaaaca attgcaacaa tccatgagca gtgctgactc aactcaggcc taaactcatg 29520 cagaccacac aaggcagatg ggctatataa acgttttcgc ttttccgttt acgatatata 29580 gtctactctt gtgcagaatg aattctcgta actacatagc acaagtagat gtagttaact 29640 ttaatctcac atagcaatct ttaatcagtg tgtaacatta gggaggactt gaaagagcca 29700 ccacattttc accgaggcca cgcggagtac gatcgagtgt acagtgaaca atgctaggga 29760 gagctgccta tatggaagag ccctaatgtg taaaattaat tttagtagtg ctatccccat 29820 gtgattttaa tagcttctta ggagaatgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 29880 nnn 29883 <210> 23 <211> 1270 <212> PRT <213> SARS-CoV2 <400> 23 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro 65 70 75 80 Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser 85 90 95 Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr 100 105 110 Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val 115 120 125 Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr His Lys 130 135 140 Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala 145 150 155 160 Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu 165 170 175 Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys 180 185 190 Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn 195 200 205 Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val 210 215 220 Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala 225 230 235 240 Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr 245 250 255 Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe 260 265 270 Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys 275 280 285 Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr 290 295 300 Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr 305 310 315 320 Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 325 330 335 Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 340 345 350 Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 355 360 365 Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 370 375 380 Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 385 390 395 400 Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 405 410 415 Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 420 425 430 Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 435 440 445 Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 450 455 460 Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 465 470 475 480 Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 485 490 495 Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 500 505 510 Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 515 520 525 Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr 530 535 540 Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln 545 550 555 560 Gln Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val Arg Asp Pro 565 570 575 Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val 580 585 590 Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu 595 600 605 Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp 610 615 620 Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe 625 630 635 640 Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser 645 650 655 Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln 660 665 670 Thr Gln Thr Asn Ser His Arg Arg Ala Arg Ser Val Ala Ser Gln Ser 675 680 685 Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr 690 695 700 Ser Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val Thr 705 710 715 720 Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 725 730 735 Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 740 745 750 Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 755 760 765 Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 770 775 780 Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 785 790 795 800 Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 805 810 815 Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 820 825 830 Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 835 840 845 Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 850 855 860 Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 865 870 875 880 Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 885 890 895 Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 900 905 910 Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 915 920 925 Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 930 935 940 Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 945 950 955 960 Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 965 970 975 Ile Leu Ala Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg 980 985 990 Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln 995 1000 1005 Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala Thr 1010 1015 1020 Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys 1025 1030 1035 1040 Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly 1045 1050 1055 Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe 1060 1065 1070 Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His Phe Pro Arg 1075 1080 1085 Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg 1090 1095 1100 Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr His Asn Thr Phe Val Ser 1105 1110 1115 1120 Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp 1125 1130 1135 Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr 1140 1145 1150 Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly 1155 1160 1165 Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn 1170 1175 1180 Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu 1185 1190 1195 1200 Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp Leu Gly 1205 1210 1215 Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Met Leu Cys 1220 1225 1230 Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys Ser Cys Gly 1235 1240 1245 Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val Leu Lys Gly 1250 1255 1260 Val Lys Leu His Tyr Thr 1265 1270 <210> 24 <211> 29832 <212> DNA <213> SARS-CoV2 <400> 24 aaggtttata ccttcccagg taacaaacca accaactttc gatctcttgt agatctgttc 60 tctaaacgaa ctttaaaatc tgtgtggctg tcactcggct gcatgcttag tgcactcacg 120 cagtataatt aataactaat tactgtcgtt gacaggacac gagtaactcg tctatcttct 180 gcaggctgct tacggtttcg tccgtgttgc agccgatcat cagcacatct aggttttgtc 240 cgggtgtgac cgaaaggtaa gatggagagc cttgtccctg gtttcaacga gaaaacacac 300 gtccaactca gtttgcctgt tttacaggtt cgcgacgtgc tcgtacgtgg ctttggagac 360 tccgtggagg aggtcttatc agaggcacgt caacatctta aagatggcac ttgtggctta 420 gtagaagttg aaaaaggcgt tttgcctcaa cttgaacagc cctatgtgtt catcaaacgt 480 tcggatgctc gaactgcacc tcatggtcat gttatggttg agctggtagc agaactcgaa 540 ggcattcagt acggtcgtag tggtgagaca cttggtgtcc ttgtccctca tgtgggcgaa 600 ataccagtgg cttaccgcaa ggttcttctt cgtaagaacg gtaataaagg agctggtggc 660 catagttacg gcgccgatct aaagtcattt gacttaggcg acgagcttgg cactgatcct 720 tatgaagatt ttcaagaaaa ctggaacact aaacatagca gtggtgttac ccgtgaactc 780 atgcgtgagc ttaacggagg ggcatacact cgctatgtcg ataacaactt ctgtggccct 840 gatggctacc ctcttgagtg cattaaagac cttctagcac gtgctggtaa agcttcatgc 900 actttgtccg aacaactgga ctttattgac actaagaggg gtgtatactg ctgccgtgaa 960 catgagcatg aaattgcttg gtacacggaa cgttctgaaa agagctatga attgcagaca 1020 ccttttgaaa ttaaattggc aaagaaattt gacatcttca atggggaatg tccaaatttt 1080 gtatttccct taaattccat aatcaagact attcaaccaa gggttgaaaa gaaaaagctt 1140 gatggcttta tgggtagaat tcgatctgtc tatccagttg cgtcaccaaa tgaatgcaac 1200 caaatgtgcc tttcaactct catgaagtgt gatcattgtg gtgaaacttc atggcagacg 1260 ggcgattttg ttaaagccac ttgcgaattt tgtggcactg agaatttgac taaagaaggt 1320 gccactactt gtggttactt accccaaaat gctgttgtta aaatttattg tccagcatgt 1380 cacaattcag aagtaggacc tgagcatagt cttgccgaat accataatga atctggcttg 1440 aaaaccattc ttcgtaaggg tggtcgcact attgcctttg gaggctgtgt gttctcttat 1500 gttggttgcc ataacaagtg tgcctattgg gttccacgtg ctagcgctaa cataggttgt 1560 aaccatacag gtgttgttgg agaaggttcc gaaggtctta atgacaacct tcttgaaata 1620 ctccaaaaag agaaagtcaa catcaatatt gttggtgact ttaaacttaa tgaagagatc 1680 gccattattt tggcatcttt ttctgcttcc acaagtgctt ttgtggaaac tgtgaaaggt 1740 ttggattata aagcattcaa acaaattgtt gaatcctgtg gtaattttaa agttacaaaa 1800 ggaaaagcta aaaaaggtgc ctggaatatt ggtgaacaga aatcaatact gagtcctctt 1860 tatgcatttg catcagaggc tgctcgtgtt gtacgatcaa ttttctcccg cactcttgaa 1920 actgctcaaa attctgtgcg tgttttacag aaggccgcta taacaatact agatggaatt 1980 tcacagtatt cactgagact cattgatgct atgatgttca catctgattt ggctactaac 2040 aatctagttg taatggccta cattacaggt ggtgttgttc agttgacttc gcagtggcta 2100 actaacatct ttggcactgt ttatgaaaaa ctcaaacccg tccttgattg gcttgaagag 2160 aagtttaagg aaggtgtaga gtttcttaga gacggttggg aaattgttaa atttatctca 2220 acctgtgctt gtgaaattgt cggtggacaa attgtcacct gtgcaaagga aattaaggag 2280 agtgttcaga cattctttaa gcttgtaaat aaatttttgg ctttgtgtgc tgactctatc 2340 attattggtg gagctaaact taaagccttg aatttaggtg aaacatttgt cacgcactca 2400 aagggattgt acagaaagtg tgttaaatcc agagaagaaa ctggcctact catgcctcta 2460 aaagccccaa aagaaattat cttcttagag ggagaaacac ttcccacaga agtgttaaca 2520 gaggaagttg tcttgaaaac tggtgattta caaccattag aacaacctac tagtgaagct 2580 gttgaagctc cattggttgg tacaccagtt tgtattaacg ggcttatgtt gctcgaaatc 2640 aaagacacag aaaagtactg tgcccttgca cctaatatga tggtaacaaa caataccttc 2700 acactcaaag gcggtgcacc aacaaaggtt acttttggtg atgacactgt gatagaagtg 2760 caaggttaca agagtgtgaa tatcactttt gaacttgatg aaaggattga taaagtactt 2820 aatgagaagt gctctgccta tacagttgaa ctcggtacag aagtaaatga gttcgcctgt 2880 gttgtggcag atgctgtcat aaaaactttg caaccagtat ctgaattact tacaccactg 2940 ggcattgatt tagatgagtg gagtatggct acatactact tatttgatga gtctggtgag 3000 tttaaattgg cttcacatat gtattgttct ttttaccctc cagatgagga tgaagaagaa 3060 ggtgattgtg aagaagaaga gtttgagcca tcaactcaat atgagtatgg tactgaagat 3120 gattaccaag gtaaaccttt ggaatttggt gccacttctg ctgctcttca acctgaagaa 3180 gagcaagaag aagattggtt agatgatgat agtcaacaaa ctgttggtca acaagacggc 3240 agtgaggaca atcagacaac tactattcaa acaattgttg aggttcaacc tcaattagag 3300 atggaactta caccagttgt tcagactatt gaagtgaata gttttagtgg ttatttaaaa 3360 cttactgaca atgtatacat taaaaatgca gacattgtgg aagaagctaa aaaggtaaaa 3420 ccaacagtgg ttgttaatgc agccaatgtt taccttaaac atggaggagg tgttgcagga 3480 gccttaaata aggctactaa caatgccatg caagttgaat ctgatgatta catagctact 3540 aatggaccac ttaaagtggg tggtagttgt gttttaagcg gacacaatct tgctaaacac 3600 tgtcttcatg ttgtcggccc aaatgttaac aaaggtgaag acattcaact tcttaagagt 3660 gcttatgaaa attttaatca gcacgaagtt ctacttgcac cattattatc agctggtatt 3720 tttggtgctg accctataca ttctttaaga gtttgtgtag atactgttcg cacaaatgtc 3780 tacttagctg tctttgataa aaatctctat gacaaacttg tttcaagctt tttggaaatg 3840 aagagtgaaa agcaagttga acaaaagatc gctgagattc ctaaagagga agttaagcca 3900 tttataactg aaagtaaacc ttcagttgaa cagagaaaac aagatgataa gaaaatcaaa 3960 gcttgtgttg aagaagttac aacaactctg gaagaaacta agttcctcac agaaaacttg 4020 ttactttata ttgacattaa tggcaatctt catccagatt ctgccactct tgttagtgac 4080 attgacatca ctttcttaaa gaaagatgct ccatatatag tgggtgatgt tgttcaagag 4140 ggtgttttaa ctgctgtggt tatacctact aaaaaggctg gtggcactac tgaaatgcta 4200 gcgaaagctt tgagaaaagt gccaacagac aattatataa ccacttaccc gggtcagggt 4260 ttaaatggtt acactgtaga ggaggcaaag acagtgctta aaaagtgtaa aagtgccttt 4320 tacattctac catctattat ctctaatgag aagcaagaaa ttcttggaac tgtttcttgg 4380 aatttgcgag aaatgcttgc acatgcagaa gaaacacgca aattaatgcc tgtctgtgtg 4440 gaaactaaag ccatagtttc aactatacag cgtaaatata agggtattaa aatacaagag 4500 ggtgtggttg attatggtgc tagattttac ttttacacca gtaaaacaac tgtagcgtca 4560 cttatcaaca cacttaacga tctaaatgaa actcttgtta caatgccact tggctatgta 4620 acacatggct taaatttgga agaagctgct cggtatatga gatctctcaa agtgccagct 4680 acagtttctg tttcttcacc tgatgctgtt acagcgtata atggttatct tacttcttct 4740 tctaaaacac ctgaagaaca ttttattgaa accatctcac ttgctggttc ctataaagat 4800 tggtcctatt ctggacaatc tacacaacta ggtatagaat ttcttaagag aggtgataaa 4860 agtgtatatt acactagtaa tcctaccaca ttccacctag atggtgaagt tatcaccttt 4920 gacaatctta agacacttct ttctttgaga gaagtgagga ctattaaggt gtttacaaca 4980 gtagacaaca ttaacctcca cacgcaagtt gtggacatgt caatgacata tggacaacag 5040 tttggtccaa cttatttgga tggagctgat gttactaaaa taaaacctca taattcacat 5100 gaaggtaaaa cattttatgt tttacctaat gatgacactc tacgtgttga ggcttttgag 5160 tactaccaca caactgatcc tagttttctg ggtaggtaca tgtcagcatt aaatcacact 5220 aaaaagtgga aatacccaca agttaatggt ttaacttcta ttaaatgggc agataacaac 5280 tgttatcttg ccactgcatt gttaacactc caacaaatag agttgaagtt taatccacct 5340 gctctacaag atgcttatta cagagcaagg gctggtgaag ctgctaactt ttgtgcactt 5400 atcttagcct actgtaataa gacagtaggt gagttaggtg atgttagaga aacaatgagt 5460 tacttgtttc aacatgccaa tttagattct tgcaaaagag tcttgaacgt ggtgtgtaaa 5520 acttgtggac aacagcagac aacccttaag ggtgtagaag ctgttatgta catgggcaca 5580 ctttcttatg aacaatttaa gaaaggtgtt cagatacctt gtacgtgtgg taaacaagct 5640 acaaaatatc tagtacaaca ggagtcacct tttgttatga tgtcagcacc acctgctcag 5700 tatgaactta agcatggtac atttacttgt gctagtgagt acactggtaa ttaccagtgt 5760 ggtcactata aacatataac ttctaaagaa actttgtatt gcatagacgg tgctttactt 5820 acaaagtcct cagaatacaa aggtcctatt acggatgttt tctacaaaga aaacagttac 5880 acaacaacca taaaaccagt tacttataaa ttggatggtg ttgtttgtac agaaattgac 5940 cctaagttgg acaattatta taagaaagac aattcttatt tcacagagca accaattgat 6000 cttgtaccaa accaaccata tccaaacgca agcttcgata attttaagtt tgtatgtgat 6060 aatatcaaat ttgctgatga tttaaaccag ttaactggtt ataagaaacc tgcttcaaga 6120 gagcttaaag ttacattttt ccctgactta aatggtgatg tggtggctat tgattataaa 6180 cactacacac cctcttttaa gaaaggagct aaattgttac ataaacctat tgtttggcat 6240 gttaacaatg caactaataa agccacgtat aaaccaaata cctggtgtat acgttgtctt 6300 tggagcacaa aaccagttga aacatcaaat tcgtttgatg tactgaagtc agaggacgcg 6360 cagggaatgg ataatcttgt ctgcgaagat ctaaaaccag tctctgaaga agtagtggaa 6420 aatcctacca tacagaaaga cgttcttgag tgtaatgtga aaactaccga agttgtagga 6480 gacattatac ttaaaccagc aaataatagt ttaaaaatta cagaagaggt tggccacaca 6540 gatctaatgg ctgcttatgt agacaattct agtcttacta ttaagaaacc taatgaatta 6600 tctagagtat taggtttgaa aacccttgct actcatggtt tagctgctgt taatagtgtc 6660 ccttgggata ctatagctaa ttatgctaag ccttttctta acaaagttgt tagtacaact 6720 actaacatag ttacacggtg tttaaaccgt gtttgtacta attatatgcc ttatttcttt 6780 actttattgc tacaattgtg tacttttact agaagtacaa attctagaat taaagcatct 6840 atgccgacta ctatagcaaa gaatactgtt aagagtgtcg gtaaattttg tctagaggct 6900 tcatttaatt atttgaagtc acctaatttt tctaaactga taaatattat aatttggttt 6960 ttactattaa gtgtttgcct aggttcttta atctactcaa ccgctgcttt aggtgtttta 7020 atgtctaatt taggcatgcc ttcttactgt actggttaca gagaaggcta tttgaactct 7080 actaatgtca ctattgcaac ctactgtact ggttctatac cttgtagtgt ttgtcttagt 7140 ggtttagatt ctttagacac ctatccttct ttagaaacta tacaaattac catttcatct 7200 tttaaatggg atttaactgc ttttggctta gttgcagagt ggtttttggc atatattctt 7260 ttcactaggt ttttctatgt acttggattg gctgcaatca tgcaattgtt tttcagctat 7320 tttgcagtac attttattag taattcttgg cttatgtggt taataattaa tcttgtacaa 7380 atggccccga tttcagctat ggttagaatg tacatcttct ttgcatcatt ttattatgta 7440 tggaaaagtt atgtgcatgt tgtagacggt tgtaattcat caacttgtat gatgtgttac 7500 aaacgtaata gagcaacaag agtcgaatgt acaactattg ttaatggtgt tagaaggtcc 7560 ttttatgtct atgctaatgg aggtaaaggc ttttgcaaac tacacaattg gaattgtgtt 7620 aattgtgata cattctgtgc tggtagtaca tttattagtg atgaagttgc gagagacttg 7680 tcactacagt ttaaaagacc aataaatcct actgaccagt cttcttacat cgttgatagt 7740 gttacagtga agaatggttc catccatctt tactttgata aagctggtca aaagacttat 7800 gaaagacatt ctctctctca ttttgttaac ttagacaacc tgagagctaa taacactaaa 7860 ggttcattgc ctattaatgt tatagttttt gatggtaaat caaaatgtga agaatcatct 7920 gcaaaatcag cgtctgttta ctacagtcag cttatgtgtc aacctatact gttactagat 7980 caggcattag tgtctgatgt tggtgatagt gcggaagttg cagttaaaat gtttgatgct 8040 tacgttaata cgttttcatc aacttttaac gtaccaatgg aaaaactcaa aacactagtt 8100 gcaactgcag aagctgaact tgcaaagaat gtgtccttag acaatgtctt atctactttt 8160 atttcagcag ctcggcaagg gtttgttgat tcagatgtag aaactaaaga tgttgttgaa 8220 tgtcttaaat tgtcacatca atctgacata gaagttactg gcgatagttg taataactat 8280 atgctcacct ataacaaagt tgaaaacatg acaccccgtg accttggtgc ttgtattgac 8340 tgtagtgcgc gtcatattaa tgcgcaggta gcaaaaagtc acaacattgc tttgatatgg 8400 aacgttaaag atttcatgtc attgtctgaa caactacgaa aacaaatacg tagtgctgct 8460 aaaaagaata acttaccttt taagttgaca tgtgcaacta ctagacaagt tgttaatgtt 8520 gtaacaacaa agatagcact taagggtggt aaaattgtta ataattggtt gaagcagtta 8580 attaaagtta cacttgtgtt cctttttgtt gctgctattt tctatttaat aacacctgtt 8640 catgtcatgt ctaaacatac tgacttttca agtgaaatca taggatacaa ggctattgat 8700 ggtggtgtca ctcgtgacat agcatctaca gatacttgtt ttgctaacaa acatgctgat 8760 tttgacacat ggtttagcca gcgtggtggt agttatacta atgacaaagc ttgcccattg 8820 attgctgcag tcataacaag agaagtgggt tttgtcgtgc ctggtttgcc tggcacgata 8880 ttacgcacaa ctaatggtga ctttttgcat ttcttaccta gagtttttag tgcagttggt 8940 aacatctgtt acacaccatc aaaacttata gagtacactg actttgcaac atcagcttgt 9000 gttttggctg ctgaatgtac aatttttaaa gatgcttctg gtaagccagt accatattgt 9060 tatgatacca atgtactaga aggttctgtt gcttatgaaa gtttacgccc tgacacacgt 9120 tatgtgctca tggatggctc tattattcaa tttcctaaca cctaccttga aggttctgtt 9180 agagtggtaa caacttttga ttctgagtac tgtaggcacg gcacttgtga aagatcagaa 9240 gctggtgttt gtgtatctac tagtggtaga tgggtactta acaatgatta ttacagatct 9300 ttaccaggag ttttctgtgg tgtagatgct gtaaatttac ttactaatat gtttacacca 9360 ctaattcaac ctattggtgc tttggacata tcagcatcta tagtagctgg tggtattgta 9420 gctatcgtag taacatgcct tgcctactat tttatgaggt ttagaagagc ttttggtgaa 9480 tacagtcatg tagttgcctt taatacttta ctattcctta tgtcattcac tgtactctgt 9540 ttaacaccag tttactcatt cttacctggt gtttattctg ttatttactt gtacttgaca 9600 ttttatctta ctaatgatgt ttctttttta gcacatattc agtggatggt tatgttcaca 9660 cctttagtac ctttctggat aacaattgct tatatcattt gtatttccac aaagcatttc 9720 tattggttct ttactaatta cctaaagaga cgtgtagtct ttaatggtgt ttcctttagt 9780 acttttgaag aagctgcgct gtgcaccttt ttgttaaata aagaaatgta tctaaagttg 9840 cgtagtgatg tgctattacc tcttacgcaa tataatagat acttagctct ttataataag 9900 tacaagtatt ttagtggagc aatggataca actagctaca gagaagctgc ttgttgtcat 9960 ctcgcaaagg ctctcaatga cttcagtaac tcaggttctg atgttcttta ccaaccacca 10020 caaacctcta tcacctcagc tgttttgcag agtggtttta gaaaaatggc attcccatct 10080 ggtaaagttg agggttgtat ggtacaagta acttgtggta caactacact taacggtctt 10140 tggcttgatg acgtagttta ctgtccaaga catgtgatct gcacctctga agacatgctt 10200 aaccctaatt atgaagattt actcattcgt aagtctaatc ataatttctt ggtacaggct 10260 ggtaatgttc aactcagggt tattggacat tctatgcaaa attgtgtact taagcttaag 10320 gttgatacag ccaatcctaa gacacctaag tataagtttg ttcgcattca accaggacag 10380 actttttcag tgttagcttg ttacaatggt tcaccatctg gtgtttacca atgtgctatg 10440 aggcccaatt tcactattaa gggttcattc cttaatggtt catgtggtag tgttggtttt 10500 aacatagatt atgactgtgt ctctttttgt tacatgcacc atatggaatt accaactgga 10560 gttcatgctg gcacagactt agaaggtaac ttttatggac cttttgttga caggcaaaca 10620 gcacaagcag ctggtacgga cacaactatt acagttaatg ttttagcttg gttgtacgct 10680 gctgttataa atggagacag gtggtttctc aatcgattta ccacaactct taatgacttt 10740 aaccttgtgg ctatgaagta caattatgaa cctctaacac aagaccatgt tgacatacta 10800 ggacctcttt ctgctcaaac tggaattgcc gttttagata tgtgtgcttc attaaaagaa 10860 ttactgcaaa atggtatgaa tggacgtacc atattgggta gtgctttatt agaagatgaa 10920 tttacacctt ttgatgttgt tagacaatgc tcaggtgtta ctttccaaag tgcagtgaaa 10980 agaacaatca agggtacaca ccactggttg ttactcacaa ttttgacttc acttttagtt 11040 ttagtccaga gtactcaatg gtctttgttc ttttttttgt atgaaaatgc ctttttacct 11100 tttgctatgg gtattattgc tatgtctgct tttgcaatga tgtttgtcaa acataagcat 11160 gcatttctct gtttgttttt gttaccttct cttgccactg tagcttattt taatatggtc 11220 tatatgcctg ctagttgggt gatgcgtatt atgacatggt tggatatggt tgatactagt 11280 ttgtctggtt ttaagctaaa agactgtgtt atgtatgcat cagctgtagt gttactaatc 11340 cttatgacag caagaactgt gtatgatgat ggtgctagga gagtgtggac acttatgaat 11400 gtcttgacac tcgtttataa agtttattat ggtaatgctt tagatcaagc catttccatg 11460 tgggctctta taatctctgt tacttctaac tactcaggtg tagttacaac tgtcatgttt 11520 ttggccaaag gtattgtttt tatgtgtgtt gagtattgcc ctattttctt cataactggt 11580 aatacacttc agtgtataat gctagtttat tgtttcttag gctatttttg tacttgttac 11640 tttggcctct tttgtttact caaccgctac tttagactga ctcttggtgt ttatgattac 11700 ttagtttcta cacaggagtt tagatatatg aattcacagg gactactccc acccaagaat 11760 agcatagatg ccttcaaact caacattaaa ttgttgggtg ttggtggcaa accttgtatc 11820 aaagtagcca ctgtacagtc taaaatgtca gatgtaaagt gcacatcagt agtcttactc 11880 tcagttttgc aacaactcag agtagaatca tcatctaaat tgtgggctca atgtgtccag 11940 ttacacaatg acattctctt agctaaagat actactgaag cctttgaaaa aatggtttca 12000 ctactttctg ttttgctttc catgcagggt gctgtagaca taaacaagct ttgtgaagaa 12060 atgctggaca acagggcaac cttacaagct atagcctcag agtttagttc ccttccatca 12120 tatgcagctt ttgctactgc tcaagaagct tatgagcagg ctgttgctaa tggtgattct 12180 gaagttgttc ttaaaaagtt gaagaagtct ttgaatgtgg ctaaatctga atttgaccgt 12240 gatgcagcca tgcaacgtaa gttggaaaag atggctgatc aagctatgac ccaaatgtat 12300 aaacaggcta gatctgagga caagagggca aaagttacta gtgctatgca gacaatgctt 12360 ttcactatgc ttagaaagtt ggataatgat gcactcaaca acattatcaa caatgcaaga 12420 gatggttgtg ttcccttgaa cataatacct cttacaacag cagccaaatt aatggttgtc 12480 ataccagact ataacacata taaaaatacg tgtgatggta caacatttac ttatgcatca 12540 gcattgtggg aaatccaaca ggttgtagat gcagatagta aaattgttca acttagtgaa 12600 attagtatgg acaattcacc taatttagca tggcctctta ttgtaacagc tttaagggcc 12660 aattctgctg tcaaattaca gaataatgag cttagtcctg ttgcactacg acagatgtct 12720 tgtgctgccg gtactacaca aactgcttgc actgatgaca atgcgttagc ttactacaac 12780 acaacaaagg gaggtaggtt tgtacttgca ctgttatccg atttacagga tttgaaatgg 12840 gctagattcc ctaagagtga tggaactggt actatctata cagaactgga accaccttgt 12900 aggtttgtta cagacacacc taaaggtcct aaagtgaagt atttatactt tattaaagga 12960 ttaaacaacc taaatagagg tatggtactt ggtagtttag ctgccacagt acgtctacaa 13020 gctggtaatg caacagaagt gcctgccaat tcaactgtat tatctttctg tgcttttgct 13080 gtagatgctg ctaaagctta caaagattat ctagctagtg ggggacaacc aatcactaat 13140 tgtgttaaga tgttgtgtac acacactggt actggtcagg caataacagt tacaccggaa 13200 gccaatatgg atcaagaatc ctttggtggt gcatcgtgtt gtctgtactg ccgttgccac 13260 atagatcatc caaatcctaa aggattttgt gacttaaaag gtaagtatgt acaaatacct 13320 acaacttgtg ctaatgaccc tgtgggtttt acacttaaaa acacagtctg taccgtctgc 13380 ggtatgtgga aaggttatgg ctgtagttgt gatcaactcc gcgaacccat gcttcagtca 13440 gctgatgcac aatcgttttt aaacgggttt gcggtgtaag tgcagcccgt cttacaccgt 13500 gcggcacagg cactagtact gatgtcgtat acagggcttt tgacatctac aatgataaag 13560 tagctggttt tgctaaattc ctaaaaacta attgttgtcg cttccaagaa aaggacgaag 13620 atgacaattt aattgattct tactttgtag ttaagagaca cactttctct aactaccaac 13680 atgaagaaac aatttataat ttacttaaag attgtccagc tgttgctaaa catgacttct 13740 ttaagtttag aatagacggt gacatggtac cacatatatc acgtcaacgt cttactaaat 13800 acacaatggc agacctcgtc tatgctttaa ggcattttga tgaaggtaat tgtgacacat 13860 taaaagaaat acttgtcaca tacaattgtt gtgatgatga ttatttcaat aaaaaggact 13920 ggtatgattt tgtagaaaac ccagatatat tacgcgtata cgccaactta ggtgaacgtg 13980 tacgccaagc tttgttaaaa acagtacaat tctgtgatgc catgcgaaat gctggtattg 14040 ttggtgtact gacattagat aatcaagatc tcaatggtaa ctggtatgat ttcggtgatt 14100 tcatacaaac cacgccaggt agtggagttc ctgttgtaga ttcttattat tcattgttaa 14160 tgcctatatt aaccttgacc agggctttaa ctgcagagtc acatgttgac actgacttaa 14220 caaagcctta cattaagtgg gatttgttaa aatatgactt cacggaagag aggttaaaac 14280 tctttgaccg ttattttaaa tattgggatc agacatacca cccaaattgt gttaactgtt 14340 tggatgacag atgcattctg cattgtgcaa actttaatgt tttattctct acagtgttcc 14400 cacttacaag ttttggacca ctagtgagaa aaatatttgt tgatggtgtt ccatttgtag 14460 tttcaactgg ataccacttc agagagctag gtgttgtaca taatcaggat gtaaacttac 14520 atagctctag acttagtttt aaggaattac ttgtgtatgc tgctgaccct gctatgcacg 14580 ctgcttctgg taatctatta ctagataaac gcactacgtg cttttcagta gctgcactta 14640 ctaacaatgt tgcttttcaa actgtcaaac ccggtaattt taacaaagac ttctatgact 14700 ttgctgtgtc taagggtttc tttaaggaag gaagttctgt tgaattaaaa cacttcttct 14760 ttgctcagga tggtaatgct gctatcagcg attatgacta ctatcgttat aatctaccaa 14820 caatgtgtga tatcagacaa ctactatttg tagttgaagt tgttgataag tactttgatt 14880 gttacgatgg tggctgtatt aatgctaacc aagtcatcgt caacaaccta gacaaatcag 14940 ctggttttcc atttaataaa tggggtaagg ctagacttta ttatgattca atgagttatg 15000 aggatcaaga tgcacttttc gcatatacaa aacgtaatgt catccctact ataactcaaa 15060 tgaatcttaa gtatgccatt agtgcaaaga atagagctcg caccgtagct ggtgtctcta 15120 tctgtagtac tatgaccaat agacagtttc atcaaaaatt attgaaatca atagccgcca 15180 ctagaggagc tactgtagta attggaacaa gcaaattcta tggtggttgg cacaacatgt 15240 taaaaactgt ttatagtgat gtagaaaacc ctcaccttat gggttgggat tatcctaaat 15300 gtgatagagc catgcctaac atgcttagaa ttatggcctc acttgttctt gctcgcaaac 15360 atacaacgtg ttgtagcttg tcacaccgtt tctatagatt agctaatgag tgtgctcaag 15420 tattgagtga aatggtcatg tgtggcggtt cactatatgt taaaccaggt ggaacctcat 15480 caggagatgc cacaactgct tatgctaata gtgtttttaa catttgtcaa gctgtcacgg 15540 ccaatgttaa tgcactttta tctactgatg gtaacaaaat tgccgataag tatgtccgca 15600 atttacaaca cagactttat gagtgtctct atagaaatag agatgttgac acagactttg 15660 tgaatgagtt ttacgcatat ttgcgtaaac atttctcaat gatgatactc tctgacgatg 15720 ctgttgtgtg tttcaatagc acttatgcat ctcaaggtct agtggctagc ataaagaact 15780 ttaagtcagt tctttattat caaaacaatg tttttatgtc tgaagcaaaa tgttggactg 15840 agactgacct tactaaagga cctcatgaat tttgctctca acatacaatg ctagttaaac 15900 agggtgatga ttatgtgtac cttccttacc cagatccatc aagaatccta ggggccggct 15960 gttttgtaga tgatatcgta aaaacagatg gtacacttat gattgaacgg ttcgtgtctt 16020 tagctataga tgcttaccca cttactaaac atcctaatca ggagtatgct gatgtctttc 16080 atttgtactt acaatacata agaaagctac atgatgagtt aacaggacac atgttagaca 16140 tgtattctgt tatgcttact aatgataaca cttcaaggta ttgggaacct gagttttatg 16200 aggctatgta cacaccgcat acagtcttac aggctgttgg ggcttgtgtt ctttgcaatt 16260 cacagacttc attaagatgt ggtgcttgca tacgtagacc attcttatgt tgtaaatgct 16320 gttacgacca tgtcatatca acatcacata aattagtctt gtctgttaat ccgtatgttt 16380 gcaatgctct aggttgtgat gtcacagatg tgactcaact ttacttagga ggtatgagct 16440 attattgtaa atcacataaa ccacccatta gttttccatt gtgtgctaat ggacaagttt 16500 ttggtttata taaaaataca tgtgttggta gcgataatgt tactgacttt aatgcaattg 16560 caacatgtga ctggacaaat gctggtgatt acattttagc taacacctgt actgaaagac 16620 tcaagctttt tgcagcagaa acgctcaaag ctactgagga gacatttaaa ctgtcttatg 16680 gtattgctac tgtacgtgaa gtgctgtctg acagagaatt acatctttca tgggaagttg 16740 gtaaacctag accaccactt aaccgaaatt atgtctttac tggttatcgt gtaactaaaa 16800 acagtaaagt acaaatagga gagtacacct ttgaaaaagg tgactatggt gatgctgttg 16860 tttaccgagg tacaacaact tacaaattaa atgttggtga ttattttgtg ctgacatcac 16920 atacagtaat gccattaagt gcacctacac tagtgccaca agagcactat gttagaatta 16980 ctggcttata cccaacactc aatatctcat atgagttttc tagcaatgtt gcaaattatc 17040 aaaaggttgg tatgcaaaag tattctacac tccagggacc acctggtact ggtaagagtc 17100 attttgctat tggcctagct ctctactacc cttctgctcg catagtgtat acagcttgct 17160 ctcatgccgc tgttgatgca ctatgtgaga aggcattaaa atatttgcct atagataaat 17220 gtagtagaat tatacctgca cgtgctcgtg tagagtgttt tgataaattc aaagtgaatt 17280 caacattaga acagtatgtc ttttgtactg taaatgcatt gcctgagacg acagcagata 17340 tagttgtctt tgatgaaatt tcaatggcca caaattatga tttgagtgtt gtcaatgcca 17400 gattacgtgc taagcactat gtgtacattg gcgaccctgc tcaattacct gcaccacgca 17460 cattgctaac taagggcaca ctagaaccag aatatttcaa ttcagtgtgt agacttatga 17520 aaactatagg tccagacatg ttcctcggaa cttgtcggcg ttgtcctgct gaaattgttg 17580 acactgtgag tgctttggtt tatgataata agcttaaagc acataaagac aaatcagctc 17640 aatgctttaa aatgttttat aagggtgtta tcacgcatga tgtttcatct gcaattaaca 17700 ggccacaaat aggcgtggta agagaattcc ttacacgtaa ccctgcttgg agaaaagctg 17760 tctttatttc accttataat tcacagaatg ctgtagcctc aaagattttg ggactaccaa 17820 ctcaaactgt tgattcatca cagggctcag aatatgacta tgtcatattc actcaaacca 17880 ctgaaacagc tcactcttgt aatgtaaaca gatttaatgt tgctattacc agagcaaaag 17940 taggcatact ttgcataatg tctgatagag acctttatga caagttgcaa tttacaagtc 18000 ttgaaattcc acgtaggaat gtggcaactt tacaagctga aaatgtaaca ggactcttta 18060 aagattgtag taaggtaatc actgggttac atcctacaca ggcacctaca cacctcagtg 18120 ttgacactaa attcaaaact gaaggtttat gtgttgacat acctggcata cctaaggaca 18180 tgacctatag aagactcatc tctatgatgg gttttaaaat gaattatcaa gttaatggtt 18240 accctaacat gtttatcacc cgcgaagaag ctataagaca tgtacgtgca tggattggct 18300 tcgatgtcga ggggtgtcat gctactagag aagctgttgg taccaattta cctttacagc 18360 taggtttttc tacaggtgtt aacctagttg ctgtacctac aggttatgtt gatacaccta 18420 ataatacaga tttttccaga gttagtgcta aaccaccgcc tggagatcaa tttaaacacc 18480 tcataccact tatgtacaaa ggacttcctt ggaatgtagt gcgtataaag attgtacaaa 18540 tgttaagtga cacacttaaa aatctctctg acagagtcgt atttgtctta tgggcacatg 18600 gctttgagtt gacatctatg aagtattttg tgaaaatagg acctgagcgc acctgttgtc 18660 tatgtgatag acgtgccaca tgcttttcca ctgcttcaga cacttatgcc tgttggcatc 18720 attctattgg atttgattac gtctataatc cgtttatgat tgatgttcaa caatggggtt 18780 ttacaggtaa cctacaaagc aaccatgatc tgtattgtca agtccatggt aatgcacatg 18840 tagctagttg tgatgcaatc atgactaggt gtctagctgt ccacgagtgc tttgttaagc 18900 gtgttgactg gactattgaa tatcctataa ttggtgatga actgaagatt aatgcggctt 18960 gtagaaaggt tcaacacatg gttgttaaag ctgcattatt agcagacaaa ttcccagttc 19020 ttcacgacat tggtaaccct aaagctatta agtgtgtacc tcaagctgat gtagaatgga 19080 agttctatga tgcacagcct tgtagtgaca aagcttataa aatagaagaa ttattctatt 19140 cttatgccac acattctgac aaattcacag atggtgtatg cctattttgg aattgcaatg 19200 tcgatagata tcctgctaat tccattgttt gtagatttga cactagagtg ctatctaacc 19260 ttaacttgcc tggttgtgat ggtggcagtt tgtatgtaaa taaacatgca ttccacacac 19320 cagcttttga taaaagtgct tttgttaatt taaaacaatt accatttttc tattactctg 19380 acagtccatg tgagtctcat ggaaaacaag tagtgtcaga tatagattat gtaccactaa 19440 agtctgctac gtgtataaca cgttgcaatt taggtggtgc tgtctgtaga catcatgcta 19500 atgagtacag attgtatctc gatgcttata acatgatgat ctcagctggc tttagcttgt 19560 gggtttacaa acaatttgat acttataacc tctggaacac ttttacaaga cttcagagtt 19620 tagaaaatgt ggcttttaat gttgtaaata agggacactt tgatggacaa cagggtgaag 19680 taccagtttc tatcattaat aacactgttt acacaaaagt tgatggtgtt gatgtagaat 19740 tgtttgaaaa taaaacaaca ttacctgtta atgtagcatt tgagctttgg gctaagcgca 19800 acattaaacc agtaccagag gtgaaaatac tcaataattt gggtgtggac attgctgcta 19860 atactgtgat ctgggactac aaaagagatg ctccagcaca tatatctact attggtgttt 19920 gttctatgac tgacatagcc aagaaaccaa ctgaaacgat ttgtgcacca ctcactgtct 19980 tttttgatgg tagagttgat ggtcaagtag acttatttag aaatgcccgt aatggtgttc 20040 ttattacaga aggtagtgtt aaaggtttac aaccatctgt aggtcccaaa caagctagtc 20100 ttaatggagt cacattaatt ggagaagccg taaaaacaca gttcaattat tataagaaag 20160 ttgatggtgt tgtccaacaa ttacctgaaa cttactttac tcagagtaga aatttacaag 20220 aatttaaacc caggagtcaa atggaaattg atttcttaga attagctatg gatgaattca 20280 ttgaacggta taaattagaa ggctatgcct tcgaacatat cgtttatgga gattttagtc 20340 atagtcagtt aggtggttta catctactga ttggactagc taaacgtttt aaggaatcac 20400 cttttgaatt agaagatttt attcctatgg acagtacagt taaaaactat ttcataacag 20460 atgcgcaaac aggttcatct aagtgtgtgt gttctgttat tgatttatta cttgatgatt 20520 ttgttgaaat aataaaatcc caagatttat ctgtagtttc taaggttgtc aaagtgacta 20580 ttgactatac agaaatttca tttatgcttt ggtgtaaaga tggccatgta gaaacatttt 20640 acccaaaatt acaatctagt caagcgtggc aaccgggtgt tgctatgcct aatctttaca 20700 aaatgcaaag aatgctatta gaaaagtgtg accttcaaaa ttatggtgat agtgcaacat 20760 tacctaaagg cataatgatg aatgtcgcaa aatatactca actgtgtcaa tatttaaaca 20820 cattaacatt agctgtaccc tataatatga gagttataca ttttggtgct ggttctgata 20880 aaggagttgc accaggtaca gctgttttaa gacagtggtt gcctacgggt acgctgcttg 20940 tcgattcaga tcttaatgac tttgtctctg atgcagattc aactttgatt ggtgattgtg 21000 caactgtaca tacagctaat aaatgggatc tcattattag tgatatgtac gaccctaaga 21060 ctaaaaatgt tacaaaagaa aatgactcta aagagggttt tttcacttac atttgtgggt 21120 ttatacaaca aaagctagct cttggaggtt ccgtggctat aaagataaca gaacattctt 21180 ggaatgctga tctttataag ctcatgggac acttcgcatg gtggacagcc tttgttacta 21240 atgtgaatgc gtcatcatct gaagcatttt taattggatg taattatctt ggcaaaccac 21300 gcgaacaaat agatggttat gtcatgcatg caaattacat attttggagg aatacaaatc 21360 caattcagtt gtcttcctat tctttatttg acatgagtaa atttcccctt aaattaaggg 21420 gtactgctgt tatgtcttta aaagaaggtc aaatcaatga tatgatttta tctcttctta 21480 gtaaaggtag acttataatt agagaaaaca acagagttgt tatttctagt gatgttcttg 21540 ttaacaacta aacgaacaat gtttgttttt cttgttttat tgccactagt ctctattcag 21600 tgtgttaatc ttacaaccag aactcaatta ccccctgcat acactaattc tttcacacgt 21660 ggtgtttatt accctgacaa agttttcaga tcctcagttt tacattcaac tcaggacttg 21720 ttcttacctt tcttttccaa tgttacttgg ttccatgcta tacatgtctc tgggaccaat 21780 ggtactaaga ggtttgataa ccctgtccta ccatttaatg atggtgttta ttttgcttcc 21840 actgagaagt ctaacataat aagaggctgg atttttggta ctactttaga ttcgaagacc 21900 cagtccctac ttattgttaa taacgctact aatgttgtta ttaaagtctg tgaatttcaa 21960 ttttgtaatg atccattttt gggtgtttat taccacaaaa acaacaaaag ttgtatggaa 22020 agtgagttca gagtttattc tagtgcgaat aattgcactt ttgaatatgt ctctcagcct 22080 tttcttatgg accttgaagg aaaacagggt aatttcaaaa atcttaggga atttgtgttt 22140 aagaatattg atggttattt taaaatatat tctaagcaca cgcctattaa tttagtgcgt 22200 gatctccctc agggtttttc ggctttagaa ccattggtag atttgccaat aggtattaac 22260 atcactaggt ttcaaacttt acttgcttta catagaagtt atttgactcc tggtgattct 22320 tcttcaggtt ggacagctgg tgctgcagct tattatgtgg gttatcttca acctaggact 22380 tttctattaa aatataatga aaatggaacc attacagatg ctgtagactg tgcacttgac 22440 cctctctcag aaacaaagtg tacgttgaaa tccttcactg tagaaaaagg aatctatcaa 22500 acttctaact ttagagtcca accaacagaa tctattgtta gatttcctaa tattacaaac 22560 ttgtgccctt ttggtgaagt ttttaacgcc accagatttg catctgttta tgcttggaac 22620 aggaagagaa tcagcaactg tgttgctgat tattctgtcc tatataattc cgcatcattt 22680 tccactttta agtgttatgg agtgtctcct actaaattaa atgatctctg ctttactaat 22740 gtctatgcag attcatttgt aattagaggt gatgaagtca gacaaatcgc tccagggcaa 22800 actggaaaga ttgctgatta taattataaa ttaccagatg attttacagg ctgcgttata 22860 gcttggaatt ctaacaatct tgattctaag gttggtggta attataatta ccggtataga 22920 ttgtttagga agtctaatct caaacctttt gagagagata tttcaactga aatctatcag 22980 gccggtagca caccttgtaa tggtgttgaa ggttttaatt gttactttcc tttacaatca 23040 tatggtttcc aacccactaa tggtgttggt taccaaccat acagagtagt agtactttct 23100 tttgaacttc tacatgcacc agcaactgtt tgtggaccta aaaagtctac taatttggtt 23160 aaaaacaaat gtgtcaattt caacttcaat ggtttaacag gcacaggtgt tcttactgag 23220 tctaacaaaa agtttctgcc tttccaacaa tttggcagag acattgctga cactactgat 23280 gctgtccgtg atccacagac acttgagatt cttgacatta caccatgttc ttttggtggt 23340 gtcagtgtta taacaccagg aacaaatact tctaaccagg ttgctgttct ttatcagggt 23400 gttaactgca cagaagtccc tgttgctatt catgcagatc aacttactcc tacttggcgt 23460 gtttattcta caggttctaa tgtttttcaa acacgtgcag gctgtttaat aggggctgaa 23520 catgtcaaca actcatatga gtgtgacata cccattggtg caggtatatg cgctagttat 23580 cagactcaga ctaattctcc tcggcgggca cgtagtgtag ctagtcaatc catcattgcc 23640 tacactatgt cacttggtgc agaaaattca gttgcttact ctaataactc tattgccata 23700 cccacaaatt ttactattag tgttaccaca gaaattctac cagtgtctat gaccaagaca 23760 tcagtagatt gtacaatgta catttgtggt gattcaactg aatgcagcaa tcttttgttg 23820 caatatggca gtttttgtac acaattaaac cgtgctttaa ctggaatagc tgttgaacaa 23880 gacaaaaaca cccaagaagt ttttgcacaa gtcaaacaaa tttacaaaac accaccaatt 23940 aaagattttg gtggttttaa tttttcacaa atattaccag atccatcaaa accaagcaag 24000 aggtcattta ttgaagatct acttttcaac aaagtgacac ttgcagatgc tggcttcatc 24060 aaacaatatg gtgattgcct tggtgatatt gctgctagag acctcatttg tgcacaaaag 24120 tttaacggcc ttactgtttt gccacctttg ctcacagatg aaatgattgc tcaatacact 24180 tctgcactgt tagcgggtac aatcacttct ggttggacct ttggtgcagg tgctgcatta 24240 caaataccat ttgctatgca aatggcttat aggtttaatg gtattggagt tacacagaat 24300 gttctctatg agaaccaaaa attgattgcc aaccaattta atagtgctat tggcaaaatt 24360 caagactcac tttcttccac agcaagtgca cttggaaaac ttcaagatgt ggtcaaccaa 24420 aatgcacaag ctttaaacac gcttgttaaa caacttagct ccaattttgg tgcaatttca 24480 agtgttttaa atgatatcct ttcacgtctt gacaaagttg aggctgaagt gcaaattgat 24540 aggttgatca caggcagact tcaaagtttg cagacatatg tgactcaaca attaattaga 24600 gctgcagaaa tcagagcttc tgctaatctt gctgctacta aaatgtcaga gtgtgtactt 24660 ggacaatcaa aaagagttga tttttgtgga aagggctatc atcttatgtc cttccctcag 24720 tcagcacctc atggtgtagt cttcttgcat gtgacttatg tccctgcaca agaaaagaac 24780 ttcacaactg ctcctgccat ttgtcatgat ggaaaagcac actttcctcg tgaaggtgtc 24840 tttgtttcaa atggcacaca ctggtttgta acacaaagga atttttatga accacaaatc 24900 attactacag acaacacatt tgtgtctggt aactgtgatg ttgtaatagg aattgtcaac 24960 aacacagttt atgatccttt gcaacctgaa ttagactcat tcaaggagga gttagataaa 25020 tattttaaga atcatacatc accagatgtt gatttaggtg acatctctgg cattaatgct 25080 tcagttgtaa acattcaaaa agaaattgac cgcctcaatg aggttgccaa gaatttaaat 25140 gaatctctca tcgatctcca agaacttgga aagtatgagc agtatataaa atggccatgg 25200 tacatttggc taggttttat agctggcttg attgccatag taatggtgac aattatgctt 25260 tgctgtatga ccagttgctg tagttgtctc aagggctgtt gttcttgtgg atcctgctgc 25320 aaatttgatg aagacgactc tgagccagtg ctcaaaggag tcaaattaca ttacacataa 25380 acgaacttat ggatttgttt atgagaatct tcacaattgg aactgtaact ttgaagcaag 25440 gtgaaatcaa ggatgctact ccttcagatt ttgttcgcgc tactgcaacg ataccgatac 25500 aagcctcact ccctttcgga tggcttattg ttggcgttgc acttcttgct gtttttcata 25560 gcgcttccaa aatcataacc ctcaaaaaga gatggcaact agcactctcc aagggtgttc 25620 actttgtttg caacttgctg ttgttgtttg taacagttta ctcacacctt ttgctcgttg 25680 ctgctggcct tgaagcccct tttctctatc tttatgcttt agtctacttc ttgcagagta 25740 taaactttgt aagaataata atgaggcttt ggctttgctg gaaatgccgt tccaaaaacc 25800 cattacttta tgatgccaac tattttcttt gctggcatac taattgttac gactattgta 25860 taccttacaa tagtgtaact tcttcaattg tcattacttc aggtgatggc acaacaagtc 25920 ctatttctga acatgactac cagattggtg gttatactga aaaatgggaa tctggagtaa 25980 aagactgtgt tgtattacac agttacttca cttcagacta ttaccagctg tactcaactc 26040 aattgagtac agacactggt gttgaacatg ttaccttctt catctacaat aaaattgttg 26100 atgagcctga agaacatgtc caaattcaca caatcgacgg ttcatccgga gttgttaatc 26160 cagtaatgga accaatttat gatgaaccga cgacgactac tagcgtgcct ttgtaagcac 26220 aagctgatga gtacgaactt atgtactcat tcgtttcgga agagacaggt acgttaatag 26280 ttaatagcgt acttcttttt cttgctttcg tggtattctt gctagttaca ctagccatcc 26340 ttactgcgct tcgattgtgt gcgtactgct gcaatattgt taacgtgagt cttgtaaaac 26400 cttcttttta cgtttactct cgtgttaaaa atctgaattc ttctagagtt cctgatcttc 26460 tggtctaaac gaactaaata ttatattagt ttttctgttt ggaactttaa ttttagccat 26520 ggtagattcc aacggtacta ttaccgttga agagcttaaa aagctccttg aacaatggaa 26580 cctagtaata ggtttcctat tccttacatg gatttgtctt ctacaatttg cctatgccaa 26640 caggaatagg tttttgtata taattaagtt aatttttctc tggctgttat ggccagtaac 26700 tttagcttgt tttgtgcttg ctgctgttta cagaataaat tggatcaccg gtggaattgc 26760 tatcgcaatg gcttgtcttg taggcttgat gtggctcagc tacttcattg cttctttcag 26820 actgtttgcg cgtacgcgtt ccatgtggtc attcaatcca gaaactaaca ttcttctcaa 26880 cgtgccactc catggcacta ttctgaccag accgcttcta gaaagtgaac tcgtaatcgg 26940 agctgtgatc cttcgtggac atcttcgtat tgctggacac catctaggac gctgtgacat 27000 caaggacctg cctaaagaaa tcactgttgc tacatcacga acgctttctt attacaaatt 27060 gggagcttcg cagcgtgtag caggtgactc aggttttgct gcatacagtc gctacaggat 27120 tggcaactat aaattaaaca cagaccattc cagtagcagt gacaatattg ctttgcttgt 27180 acagtaagtg acaacagatg tttcatctcg ttgactttca ggttactata gcagagatat 27240 tactaattat tatgaggact tttaaagttt ccatttggaa tcttgattac atcataaacc 27300 tcataattaa aaatttatct aagtcactaa ctgagaataa atattctcaa ttagatgaag 27360 agcaaccaat ggagattgat taaacgaaca tgaaaattat tcttttcttg gcactgataa 27420 cactcgctac ttgtgagctt tatcactacc aagagtgtgt tagaggtaca acagtacttt 27480 taaaagaacc ttgctcttct ggaacatacg agggcaattc accatttcat cctctagctg 27540 ataacaaatt tgcactgact tgctttagca ctcaatttgc ttttgcttgt cctgacggcg 27600 taaaacacgt ctatcagtta cgtgccagat cagtttcacc taaactgttc atcagacaag 27660 aggaagttca agaactttac tctccaattt ttcttattgt tgcggcaata gtgtttataa 27720 cactttgctt cacactcaaa agaaagacag aatgattgaa ctttcattaa ttgacttcta 27780 tttgtgcttt ttagcctttc tgctattcct tgttttaatt atgcttatta tcttttggtt 27840 ctcacttgaa ctgcaagatc ataatgaaac ttgtcacgcc taaacgaaca tgaaatttct 27900 tgttttctta ggaatcatca caactgtagc tgcatttcac caagaatgta gtttacagtc 27960 atgtactcaa catcaaccat atgtagttga tgacccgtgt cctattcact tctattctaa 28020 atggtatatt agagtaggag ctagaaaatc agcaccttta attgaattgt gcgtggatga 28080 ggctggttct aaatcaccca ttcagtacat cgatatcggt aattatacag tttcctgttt 28140 accttttaca attaattgcc aggaacctaa attgggtagt cttgtagtgc gttgttcgtt 28200 ctatgaagac tttttagagt atcatgacgt tcgtgttgtt ttagatttca tctaaacgaa 28260 caaactataa tgtctgataa tggaccccaa aatcagcgaa atgcaccccg cattacgttt 28320 ggtggaccct cagattcaac tggcagtaac cagaatggag aacgcagtgg ggcgcgatca 28380 aaacaacgtc ggccccaagg tttacccaat aatactgcgt cttggttcac cgctctcact 28440 caacatggca aggaagacct taaattccct cgaggacaag gcgttccaat taacaccaat 28500 agcagtccag atgaccaaat tggctactac cgaagagcta ccagacgaat tcgtggtggt 28560 gacggtaaaa tgaaagatct cagtccaaga tggtatttct actacctagg aactgggcca 28620 gaagctggac ttccctatgg tgctaacaaa gacggcatca tatgggttgc aactgaggga 28680 gccttgaata caccaaaaga tcacattggc acccgcaatc ctgctaacaa tgctgcaatc 28740 gtgctacaac ttcctcaagg aacaacattg ccaaaaggct tctacgcaga agggagcaga 28800 ggcggcagtc aagcctcttc tcgttcctca tcacgtagtc gcaacagttc aagaaattca 28860 actccaggca gcagtagggg aatttctcct gctagaatgg ctggcaatgg cggtgatgct 28920 gctcttgctt tgctgctgct tgacagattg aaccagcttg agagcaaaat gtctggtaaa 28980 ggccaacaac aacaaggcca aactgtcact aagaaatctg ctgctgaggc ttctaagaag 29040 cctcggcaaa aacgtactgc cactaaagca tacaatgtaa cacaagcttt cggcagacgt 29100 ggtccagaac aaacccaagg aaattttggg gaccaggaac taatcagaca aggaactgat 29160 tacaaacatt ggccgcaaat tgcacaattt gcccccagcg cttcagcgtt cttcggaatg 29220 tcgcgcattg gcatggaagt cacaccttcg ggaacgtggt tgacctacac aggtgccatc 29280 aaattggatg acaaagatcc aaatttcaaa gatcaagtca ttttgctgaa taagcatatt 29340 gacgcataca aaacatttcc accaacagag cctaaaaagg acaaaaagaa gaaggctgat 29400 gaaactcaag ccttaccgca gagacagaag aaacagcaaa ctgtgactct tcttcctgct 29460 gcagatttgg atgatttctc caaacaattg caacaatcca tgagcagtgc tgactcaact 29520 caggcctaaa ctcatgcaga ccacacaagg cagatgggct atataaacgt tttcgctttt 29580 ccgtttacga tatatagtct actcttgtgc agaatgaatt ctcgtaacta catagcacaa 29640 gtagatgtag ttaactttaa tctcacatag caatctttaa tcagtgtgta acattaggga 29700 ggacttgaaa gagccaccac attttcaccg aggccacgcg gagtacgatc gagtgtacag 29760 tgaacaatgc tagggagagc tgcctatatg gaagagccct aatgtgtaaa attaatttta 29820 gtagtgctat cc 29832 <210> 25 <211> 1273 <212> PRT <213> SARS-CoV2 <400> 25 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ile Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Cys Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 225 230 235 240 Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 245 250 255 Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 260 265 270 Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 275 280 285 Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 290 295 300 Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 305 310 315 320 Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 325 330 335 Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 340 345 350 Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 355 360 365 Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 370 375 380 Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 385 390 395 400 Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 405 410 415 Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 420 425 430 Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 435 440 445 Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 450 455 460 Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 465 470 475 480 Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 485 490 495 Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 500 505 510 Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 515 520 525 Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 530 535 540 Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 545 550 555 560 Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 565 570 575 Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 580 585 590 Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 595 600 605 Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 610 615 620 His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 625 630 635 640 Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 645 650 655 Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 660 665 670 Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 675 680 685 Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 690 695 700 Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 705 710 715 720 Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 725 730 735 Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 740 745 750 Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 755 760 765 Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 770 775 780 Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 785 790 795 800 Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 805 810 815 Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 820 825 830 Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 835 840 845 Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 850 855 860 Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 865 870 875 880 Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 885 890 895 Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 900 905 910 Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 915 920 925 Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 930 935 940 Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 945 950 955 960 Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 965 970 975 Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 980 985 990 Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 995 1000 1005 Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 1010 1015 1020 Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 1025 1030 1035 1040 Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 1045 1050 1055 Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 1060 1065 1070 Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 1075 1080 1085 Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 1090 1095 1100 Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 1105 1110 1115 1120 Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 1125 1130 1135 Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 1140 1145 1150 Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 1155 1160 1165 Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 1170 1175 1180 Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 1185 1190 1195 1200 Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 1205 1210 1215 Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 1220 1225 1230 Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 1235 1240 1245 Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 1250 1255 1260 Leu Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 26 <211> 29816 <212> DNA <213> SARS-CoV2 <400> 26 actttcgatc tcttgtagat ctgttctcta aacgaacttt aaaatctgtg tggctgtcac 60 tcggctgcat gcttagtgca ctcacgcagt ataattaata actaattact gtcgttgaca 120 ggacacgagt aactcgtcta tcttctgcag gctgcttacg gtttcgtccg tgttgcagcc 180 gatcatcagc acatctaggt tttgtccggg tgtgaccgaa aggtaagatg gagagccttg 240 tccctggttt caacgagaaa acacacgtcc aactcagttt gcctgtttta caggttcgcg 300 acgtgctcgt acgtggcttt ggagactccg tggaggaggt cttatcagag gcacgtcaac 360 atcttaaaga tggcacttgt ggcttagtag aagttgaaaa aggcgttttg cctcaacttg 420 aacagcccta tgtgttcatc aaacgttcgg atgctcgaac tgcacctcat ggtcatgtta 480 tggttgagct ggtagcagaa ctcgaaggca ttcagtacgg tcgtagtggt gagacacttg 540 gtgtccttgt ccctcatgtg ggcgaaatac cagtggctta ccgcaaggtt cttcttcgta 600 agaacggtaa taaaggagct ggtggccata gttacggcgc cgatctaaag tcatttgact 660 taggcgacga gcttggcact gatccttatg aagattttca agaaaactgg aacactaaac 720 atagcagtgg tgttacccgt gaactcatgc gtgagcttaa cggaggggca tacactcgct 780 atgtcgataa caacttctgt ggccctgatg gctaccctct tgagtgcatt aaagaccttc 840 tagcacgtgc tggtaaagct tcatgcactt tgtccgaaca actggacttt attgacacta 900 agaggggtgt atactgctgc cgtgaacatg agcatgaaat tgcttggtac acggaacgtt 960 ctgaaaagag ctatgaattg cagacacctt ttgaaattaa attggcaaag aaatttgaca 1020 tcttcaatgg ggaatgtcca aattttgtat ttcccttaaa ttccataatc aagactattc 1080 aaccaagggt tgaaaagaaa aagcttgatg gctttatggg tagaattcga tctgtctatc 1140 cagttgcgtc accaaatgaa tgcaaccaaa tgtgcctttc aactctcatg aagtgtgatc 1200 attgtggtga aacttcatgg cagacgggcg attttgttaa agccacttgc gaattttgtg 1260 gcactgagaa tttgactaaa gaaggtgcca ctacttgtgg ttacttaccc caaaatgctg 1320 ttgttaaaat ttattgtcca gcatgtcaca attcagaagt aggacctgag catagtcttg 1380 ccgaatacca taatgaatct ggcttgaaaa ccattcttcg taagggtggt cgcactattg 1440 cctttggagg ctgtgtgttc tcttatgttg gttgccataa caagtgtgcc tattgggttc 1500 cacgtgctag cgctaacata ggttgtaacc atacaggtgt tgttggagaa ggttccgaag 1560 gtcttaatga caaccttctt gaaatactcc aaaaagagaa agtcaacatc aatattgttg 1620 gtgactttaa acttaatgaa gagatcgcca ttattttggc atctttttct gcttccacaa 1680 gtgcttttgt ggaaactgtg aaaggtttgg attataaagc attcaaacaa attgttgaat 1740 cctgtggtaa ttttaaagtt acaaaaggaa aagctaaaaa aggtgcctgg aatattggtg 1800 aacagaaatc aatactgagt cctctttatg catttgcatc agaggctgct cgtgttgtac 1860 gatcaatttt ctcccgcact cttgaaactg ctcaaaattc tgtgcgtgtt ttacagaagg 1920 ccgctataac aatactagat ggaatttcac agtattcact gagactcatt gatgctatga 1980 tgttcacatc tgatttggct actaacaatc tagttgtaat ggcctacatt acaggtggtg 2040 ttgttcagtt gacttcgcag tggctaacta acatctttgg cactgtttat gaaaaactca 2100 aacccgtcct tgattggctt gaagagaagt ttaaggaagg tgtagagttt cttagagacg 2160 gttgggaaat tgttaaattt atctcaacct gtgcttgtga aattgtcggt ggacaaattg 2220 tcacctgtgc aaaggaaatt aaggagagtg ttcagacatt ctttaagctt gtaaataaat 2280 ttttggcttt gtgtgctgac tctatcatta ttggtggagc taaacttaaa gccttgaatt 2340 taggtgaaac atttgttacg cactcaaagg gattgtacag aaagtgtgtt aaatccagag 2400 aagaaactgg cctactcatg cctctaaaag ccccaaaaga aattatcttc ttagagggag 2460 aaacacttcc cacagaagtg ttaacagagg aagttgtctt gaaaactggt gatttacaac 2520 cattagaaca acctactagt gaagctgttg aagctccact ggttggtaca ccagtttgta 2580 ttaacgggct tatgttgctc gaaatcaaag acacagaaaa gtactgtgcc cttgcaccta 2640 atatgatggt aacaaacaat accttcacac tcaaaggcgg tgcaccaaca aaggttactt 2700 ttggtgatga cactgtgata gaagtgcaag gttacaagag tgtgaatatc acttttgaac 2760 ttgatgaaag gattgataaa gtacttaatg agaagtgctc tgcctataca gttgaactcg 2820 gtacagaagt aaatgagttc gcctgtgttg tggcagatgc tgtcataaaa actttgcaac 2880 cagtatctga attacttaca ccactgggca ttgatttaga tgagtggagt atggctacat 2940 actacttatt tgatgagtct ggtgagttta aattggcttc acatatgtat tgttcttttt 3000 accctccaga tgaggatgaa gaagaaggtg attgtgaaga agaagagttt gagccatcaa 3060 ctcaatatga gtatggtact gaagatgatt accaaggtaa acctttggaa tttggtgcca 3120 cttctgctgc tcttcaacct gaagaagagc aagaagaaga ttggttagat gatgatagtc 3180 aacaaactgt tggtcaacaa gacggcagtg aggacaatca gacaactact attcaaacaa 3240 ttgttgaggt tcaacctcaa ttagagatgg aacttacacc agttgttcag actattgaag 3300 tgaatagttt tagtggttat ttaaaactta ctgacaatgt atacattaaa aatgcagaca 3360 ttgtggaaga agctaaaaag gtaaaaccaa cagtggttgt taatgcagcc aatgtttacc 3420 ttaaacatgg aggaggtgtt gcaggagcct taaataaggc tactaacaat gccatgcaag 3480 ttgaatctga tgattacata gctactaatg gaccacttaa agtgggtggt agttgtgttt 3540 taagcggaca caatcttgct aaacactgtc ttcatgttgt cggcccaaat gttaacaaag 3600 gtgaagacat tcaacttctt aagagtgctt atgaaaattt taatcagcac gaagttctac 3660 ttgcaccatt attatcagct ggtatttttg gtgctgaccc tatacattct ttaagagttt 3720 gtgtagatac tgttcgcaca aatgtctact tagctgtctt tgataaaaat ctctatgaca 3780 aacttgtttc aagctttttg gaaatgaaga gtgaaaagca agttgaacaa aagatcgctg 3840 agattcctaa agaggaagtt aagccattta taactgaaag taaaccttca gttgaacaga 3900 gaaaacaaga tgataagaaa atcaaagctt gtgttgaaga agttacaaca actctggaag 3960 aaactaagtt cctcacagaa aacttgttac tttatattga cattaatggc aatcttcatc 4020 cagattctgc cactcttgtt agtgacattg acatcacttt cttaaagaaa gatgctccat 4080 atatagtggg tgatgttgtt caagagggtg ttttaactgc tgtggttata cctactaaaa 4140 aggctggtgg cactactgaa atgctagcga aagctttgag aaaagtgcca acagacaatt 4200 atataaccac ttacccgggt cagggtttaa atggttacac tgtagaggag gcaaagacag 4260 tgcttaaaaa gtgtaaaagt gccttttaca ttctaccatc tattatctct aatgagaagc 4320 aagaaattct tggaactgtt tcttggaatt tgcgagaaat gcttgcacat gcagaagaaa 4380 cacgcaaatt aatgcctgtc tgtgtggaaa ctaaagccat agtttcaact atacagcgta 4440 aatataaggg tattaaaata caagagggtg tggttgatta tggtgctaga ttttactttt 4500 acaccagtaa aacaactgta gcgtcactta tcaacacact taacgatcta aatgaaactc 4560 ttgttacaat gccacttggc tatgtaacac atggcttaaa tttggaagaa gctgctcggt 4620 atatgagatc tctcaaagtg ccagctacag tttctgtttc ttcacctgat gctgttacag 4680 cgtataatgg ttatcttact tcttcttcta aaacacctga agaacatttt attgaaacca 4740 tctcacttgc tggttcctat aaagattggt cctattctgg acaatctaca caactaggta 4800 tagaatttct taagagaggt gataaaagtg tatattacac tagtaatcct accacattcc 4860 acctagatgg tgaagttatc acctttgaca atcttaagac acttctttct ttgagagaag 4920 tgaggactat taaggtgttt acaacagtag acaacattaa cctccacacg caagttgtgg 4980 acatgtcaat gacatatgga caacagtttg gtccaactta tttggatgga gctgatgtta 5040 ctaaaataaa acctcataat tcacatgaag gtaaaacatt ttatgtttta cctaatgatg 5100 acactctacg tgttgaggct tttgagtact accacacaac tgatcctagt tttctgggta 5160 ggtacatgtc agcattaaat cacactaaaa agtggaaata cccacaagtt aatggtttaa 5220 cttctattaa atgggcagat aacaactgtt atcttgccac tgcattgtta acactccaac 5280 aaatagagtt gaagtttaat ccacctgctc tacaagatgc ttattacaga gcaagggctg 5340 gtgaagctgc taacttttgt gcacttatct tagcctactg taataagaca gtaggtgagt 5400 taggtgatgt tagagaaaca atgagttact tgtttcaaca tgccaattta gattcttgca 5460 aaagagtctt gaacgtggtg tgtaaaactt gtggacaaca gcagacaacc cttaagggtg 5520 tagaagctgt tatgtacatg ggcacacttt cttatgaaca atttaagaaa ggtgttcaga 5580 taccttgtac gtgtggtaaa caagctacaa aatatctagt acaacaggag tcaccttttg 5640 ttatgatgtc agcaccacct gctcagtatg aacttaagca tggtacattt acttgtgcta 5700 gtgagtacac tggtaattac cagtgtggtc actataaaca tataacttct aaagaaactt 5760 tgtattgcat agacggtgct ttacttacaa agtcctcaga atacaaaggt cctattacgg 5820 atgttttcta caaagaaaac agttacacaa caaccataaa accagttact tataaattgg 5880 atggtgttgt ttgtacagaa attgacccta agttggacaa ttattataag aaagacaatt 5940 cttatttcac agagcaacca attgatcttg taccaaacca accatatcca aacgcaagct 6000 tcgataattt taagtttgta tgtgataata tcaaatttgc tgatgattta aaccagttaa 6060 ctggttataa gaaacctgct tcaagagagc ttaaagttac atttttccct gacttaaatg 6120 gtgatgtggt ggctattgat tataaacact acacaccctc ttttaagaaa ggagctaaat 6180 tgttacataa acctattgtt tggcatgtta acaatgcaac taataaagcc acgtataaac 6240 caaatacctg gtgtatacgt tgtctttgga gcacaaaacc agttgaaaca tcaaattcgt 6300 ttgatgtact gaagtcagag gacgcgcagg gaatggataa tcttgcctgc gaagatctaa 6360 aaccagtctc tgaagaagta gtggaaaatc ctaccataca gaaagacgtt cttgagtgta 6420 atgtgaaaac taccgaagtt gtaggagaca ttatacttaa accagcaaat aatagtttaa 6480 aaattacaga agaggttggc cacacagatc taatggctgc ttatgtagac aattctagtc 6540 ttactattaa gaaacctaat gaattatcta gagtattagg tttgaaaacc cttgctactc 6600 atggtttagc tgctgttaat agtgtccctt gggatactat agctaattat gctaagcctt 6660 ttcttaacaa agttgttagt acaactacta acatagttac acggtgttta aaccgtgttt 6720 gtactaatta tatgccttat ttctttactt tattgctaca attgtgtact tttactagaa 6780 gtacaaattc tagaattaaa gcatctatgc cgactactat agcaaagaat actgttaaga 6840 gtgtcggtaa attttgtcta gaggcttcat ttaattattt gaagtcacct aatttttcta 6900 aactgataaa tattataatt tggtttttac tattaagtgt ttgcctaggt tctttaatct 6960 actcaaccgc tgctttaggt gttttaatgt ctaatttagg catgccttct tactgtactg 7020 gttacagaga aggctatttg aactctacta atgtcactat tgcaacctac tgtactggtt 7080 ctataccttg tagtgtttgt cttagtggtt tagattcttt agacacctat ccttctttag 7140 aaactataca aattaccatt tcatctttta aatgggattt aactgctttt ggcttagttg 7200 cagagtggtt tttggcatat attcttttca ctaggttttt ctatgtactt ggattggctg 7260 caatcatgca attgtttttc agctattttg cagtacattt tattagtaat tcttggctta 7320 tgtggttaat aattaatctt gtacaaatgg ccccgatttc agctatggtt agaatgtaca 7380 tcttctttgc atcattttat tatgtatgga aaagttatgt gcatgttgta gacggttgta 7440 attcatcaac ttgtatgatg tgttacaaac gtaatagagc aacaagagtc gaatgtacaa 7500 ctattgttaa tggtgttaga aggtcctttt atgtctatgc taatggaggt aaaggctttt 7560 gcaaactaca caattggaat tgtgttaatt gtgatacatt ctgtgctggt agtacattta 7620 ttagtgatga agttgcgaga gacttgtcac tacagtttaa aagaccaata aatcctactg 7680 accagtcttc ttacatcgtt gatagtgtta cagtgaagaa tggttccatc catctttact 7740 ttgataaagc tggtcaaaag acttatgaaa gacattctct ctctcatttt gttaacttag 7800 acaacctgag agctaataac actaaaggtt cattgcctat taatgttata gtttttgatg 7860 gtaaatcaaa atgtgaagaa tcatctgcaa aatcagcgtc tgtttactac agtcagctta 7920 tgtgtcaacc tatactgtta ctagatcagg cattagtgtc tgatgttggt gatagtgcgg 7980 aagttgcagt taaaatgttt gatgcttacg ttaatacgtt ttcatcaact tttaacgtac 8040 caatggaaaa actcaaaaca ctagttgcaa ctgcagaagc tgaacttgca aagaatgtgt 8100 ccttagacaa tgtcttatct acttttattt cagcagctcg gcaagggttt gttgattcag 8160 atgtagaaac taaagatgtt gttgaatgtc ttaaattgtc acatcaatct gacatagaag 8220 ttactggcga tagttgtaat aactatatgc tcacctataa caaagttgaa aacatgacac 8280 cccgtgacct tggtgcttgt attgactgta gtgcgcgtca tattaatgcg caggtagcaa 8340 aaagtcacaa cattgctttg atatggaacg ttaaagattt catgtcattg tctgaacaac 8400 tacgaaaaca aatacgtagt gctgctaaaa agaataactt accttttaag ttgacatgtg 8460 caactactag acaagttgtt aatgttgtaa caacaaagat agcacttaag ggtggtaaaa 8520 ttgttaataa ttggttgaag cagttaatta aagttacact tgtgttcctt tttgttgctg 8580 ctattttcta tttaataaca cctgttcatg tcatgtctaa acatactgac ttttcaagtg 8640 aaatcatagg atacaaggct attgatggtg gtgtcactcg tgacatagca tctacagata 8700 cttgttttgc taacaaacat gctgattttg acacatggtt tagccagcgt ggtggtagtt 8760 atactaatga caaagcttgc ccattgattg ctgcagtcat aacaagagaa gtgggttttg 8820 tcgtgcctgg tttgcctggc acgatattac gcacaactaa tggtgacttt ttgcatttct 8880 tacctagagt ttttagtgca gttggtaata tctgttacac accatcaaaa cttatagagt 8940 acactgactt tgcaacatca gcttgtgttt tggctgctga atgtacaatt tttaaagatg 9000 cttctggtaa gccagtacca tattgttatg ataccaatgt actagaaggt tctgttgctt 9060 atgaaagttt acgccctgac acacgttatg tgctcatgga tggctctatt attcaatttc 9120 ctaacaccta ccttgaaggt tctgttagag tggtaacaac ttttgattct gagtactgta 9180 ggcacggcac ttgtgaaaga tcagaagctg gtgtttgtgt atctactagt ggtagatggg 9240 tacttaacaa tgattattac agatctttac caggagtttt ctgtggtgta gatgctgtaa 9300 atttacttac taatatgttt acaccactaa ttcaacctat tggtgctttg gacatatcag 9360 catctatagt agctggtggt attgtagcta tcgtagtaac atgccttgcc tactatttta 9420 tgaggtttag aagagctttt ggtgaataca gtcatgtagt tgcctttaat actttactat 9480 tccttatgtc attcactgta ctctgtttaa caccagttta ctcattctta cctggtgttt 9540 attctgttat ttacttgtac ttgacatttt atcttactaa tgatgtttct tttttagcac 9600 atattcagtg gatggttatg ttcacacctt tagtaccttt ctggataaca attgcttata 9660 tcatttgtat ttccacaaag catttctatt ggttctttag taattaccta aagagacgtg 9720 tagtctttaa tggtgtttcc tttagtactt ttgaagaagc tgcgctgtgc acctttttgt 9780 taaataaaga aatgtatcta aagttgcgta gtgatgtgct attacctctt acgcaatata 9840 atagatactt agctctttat aataagtaca agtattttag tggagcaatg gatacaacta 9900 gctacagaga agctgcttgt tgtcatctcg caaaggctct caatgacttc agtaactcag 9960 gttctgatgt tctttaccaa ccaccacaaa cctctatcac ctcagctgtt ttgcagagtg 10020 gttttagaaa aatggcattc ccatctggta aagttgaggg ttgtatggta caagtaactt 10080 gtggtacaac tacacttaac ggtctttggc ttgatgacgt agtttactgt ccaagacatg 10140 tgatctgcac ctctgaagac atgcttaacc ctaattatga agatttactc attcgtaagt 10200 ctaatcataa tttcttggta caggctggta atgttcaact cagggttatt ggacattcta 10260 tgcaaaattg tgtacttaag cttaaggttg atacagccaa tcctaagaca cctaagtata 10320 agtttgttcg cattcaacca ggacagactt tttcagtgtt agcttgttac aatggttcac 10380 catctggtgt ttaccaatgt gctatgaggc ccaatttcac tattaagggt tcattcctta 10440 atggttcatg tggtagtgtt ggttttaaca tagattatga ctgtgtctct ttttgttaca 10500 tgcaccatat ggaattacca actggagttc atgctggcac agacttagaa ggtaactttt 10560 atggaccttt tgttgacagg caaacagcac aagcagctgg tacggacaca actattacag 10620 ttaatgtttt agcttggttg tacgctgctg ttataaatgg agacaggtgg tttctcaatc 10680 gatttaccac aactcttaat gactttaacc ttgtggctat gaagtacaat tatgaacctc 10740 taacacaaga ccatgttgac atactaggac ctctttctgc tcaaactgga attgccgttt 10800 tagatatgtg tgcttcatta aaagaattac tgcaaaatgg tatgaatgga cgtaccatat 10860 tgggtagtgc tttattagaa gatgaattta caccttttga tgttgttaga caatgctcag 10920 gtgttacttt ccaaagtgca gtgaaaagaa caatcaaggg tacacaccac tggttgttac 10980 tcacaatttt gacttcactt ttagttttag tccagagtac tcaatggtct ttgttctttt 11040 ttttgtatga aaatgccttt ttaccttttg ctatgggtat tattgctatg tctgcttttg 11100 caatgatgtt tgtcaaacat aagcatgcat ttctctgttt gtttttgtta ccttctcttg 11160 ccactgtagc ttattttaat atggtctata tgcctgctag ttgggtgatg cgtattatga 11220 catggttgga tatggttgat actagtttgt ctggttttaa gctaaaagac tgtgttatgt 11280 atgcatcagc tgtagtgtta ctaatcctta tgacagcaag aactgtgtat gatgatggtg 11340 ctaggagagt gtggacactt atgaatgtct tgacactcgt ttataaagtt tattatggta 11400 atgctttaga tcaagccatt tccatgtggg ctcttataat ctctgttact tctaactact 11460 caggtgtagt tacaactgtc atgtttttgg ccagaggtat tgtttttatg tgtgttgagt 11520 attgccctat tttcttcata actggtaata cacttcagtg tataatgcta gtttattgtt 11580 tcttaggcta tttttgtact tgttactttg gcctcttttg tttactcaac cgctacttta 11640 gactgactct tggtgtttat gattacttag tttctacaca ggagtttaga tatatgaatt 11700 cacagggact actcccaccc aagaatagca tagatgcctt caaactcaac attaaattgt 11760 tgggtgttgg tggcaaacct tgtatcaaag tagccactgt acagtctaaa atgtcagatg 11820 taaagtgcac atcagtagtc ttactctcag ttttgcaaca actcagagta gaatcatcat 11880 ctaaattgtg ggctcaatgt gtccagttac acaatgacat tctcttagct aaagatacta 11940 ctgaagcctt tgaaaaaatg gtttcactac tttctgtttt gctttccatg cagggtgctg 12000 tagacataaa caagctttgt gaagaaatgc tggacaacag ggcaacctta caagctatag 12060 cttcagagtt tagttccctt ccatcatatg cagcttttgc tactgctcaa gaagcttatg 12120 agcaggctgt tgctaatggt gattctgaag ttgttcttaa aaagttgaag aagtctttga 12180 atgtggctaa atctgaattt gaccgtgatg cagccatgca acgtaagttg gaaaagatgg 12240 ctgatcaagc tatgacccaa atgtataaac aggctagatc tgaggacaag agggcaaaag 12300 ttactagtgc tatgcagaca atgcttttca ctatgcttag aaagttggat aatgatgcac 12360 tcaacaacat tatcaacaat gcaagagatg gttgtgttcc cttgaacata atacctctta 12420 caacagcagc caaactaatg gttgtcatac cagactataa cacatataaa aatacgtgtg 12480 atggtacaac atttacttat gcatcagcat tgtgggaaat ccaacaggtt gtagatgcag 12540 atagtaaaat tgttcaactt agtgaaatta gtatggacaa ttcacctaat ttagcatggc 12600 ctcttattgt aacagcttta agggccaatt ctgctgtcaa attacagaat aatgagctta 12660 gtcctgttgc actacgacag atgtcttgtg ctgccggtac tacacaaact gcttgcactg 12720 atgacaatgc gttagcttac tacaacacaa caaagggagg taggtttgta cttgcactgt 12780 tatccgattt acaggatttg aaatgggcta gattccctaa gagtgatgga actggtactg 12840 tctatacaga actggaacca ccttgtaggt ttgttacaga cacacctaaa ggtcctaaag 12900 tgaagtattt atactttatt aaaggattaa acaacctaaa tagaggtatg gtacttggta 12960 gtttagctgc cacagtacgt ctacaagctg gtaatgcaac agaagtgcct gccaattcaa 13020 ctgtattatc tttctgtgct tttgctgtag atgctgctaa agcttacaaa gattatctag 13080 ctagtggggg acaaccaatc actaattgtg ttaagatgtt gtgtacacac actggtactg 13140 gtcaggcaat aacagttaca ccggaagcca atatggatca agaatccttt ggtggtgcat 13200 cgtgttgtct gtactgccgt tgccacatag atcatccaaa tcctaaagga ttttgtgact 13260 taaaaggtaa gtatgtacaa atacctacaa cttgtgctaa tgaccctgtg ggttttacac 13320 ttaaaaacac agtctgtacc gtctgcggta tgtggaaagg ttatggctgt agttgtgatc 13380 aactccgcga acccatgctt cagtcagctg atgcacaatc gtttttaaac gggtttgcgg 13440 tgtaagtgca gcccgtctta caccgtgcgg cacaggcact agtactgatg tcgtatacag 13500 ggcttttgac atctacaatg ataaagtagc tggttttgct aaattcctaa aaactaattg 13560 ttgtcgcttc caagaaaagg acgaagatga caatttaatt gattcttact ttgtagttaa 13620 gagacacact ttctctaact accaacatga agaaacaatt tataatttac ttaaggattg 13680 tccagctgtt gctaaacatg acttctttaa gtttagaata gacggtgaca tggtaccaca 13740 tatatcacgt caacgtctta ctaaatacac aatggcagac ctcgtctatg ctttaaggca 13800 ttttgatgaa ggtaattgtg acacattaaa agaaatactt gtcacataca attgttgtga 13860 tgatgattat ttcaataaaa aggactggta tgattttgta gaaaacccag atatattacg 13920 cgtatacgcc aacttaggtg aacgtgtacg ccaagctttg ttaaaaacag tacaattctg 13980 tgatgccatg cgaaatgctg gtattgttgg tgtactgaca ttagataatc aagatctcaa 14040 tggtaactgg tatgatttcg gtgatttcat acaaaccacg ccaggtagtg gagttcctgt 14100 tgtagattct tattattcat tgttaatgcc tatattaacc ttgaccaggg ctttaactgc 14160 agagtcacat gttgacactg acttaacaaa gccttacatt aagtgggatt tgttaaaata 14220 tgacttcacg gaagagaggt taaaactctt tgaccgttat tttaaatatt gggatcagac 14280 ataccaccca aattgtgtta actgtttgga tgacagatgc attctgcatt gtgcaaactt 14340 taatgtttta ttctctacag tgttcccact tacaagtttt ggaccactag tgagaaaaat 14400 atttgttgat ggtgttccat ttgtagtttc aactggatac cacttcagag agctaggtgt 14460 tgtacataat caggatgtaa acttacatag ctctagactt agttttaagg aattacttgt 14520 gtatgctgct gaccctgcta tgcacgctgc ttctggtaat ctattactag ataaacgcac 14580 tacgtgcttt tcagtagctg cacttactaa caatgttgct tttcaaactg tcaaacccgg 14640 taattttaac aaagacttct atgactttgc tgtgtctaag ggtttcttta aggaaggaag 14700 ttctgttgaa ttaaaacact tcttctttgc tcaggatggt aatgctgcta tcagcgatta 14760 tgactactat cgttataatc taccaacaat gtgtgatatc agacaactac tatttgtagt 14820 tgaagttgtt gataagtact ttgattgtta cgatggtggc tgtattaatg ctaaccaagt 14880 catcgtcaac aacctagaca aatcagctgg ttttccattt aataaatggg gtaaggctag 14940 actttattat gattcaatga gttatgagga tcaagatgca cttttcgcat atacaaaacg 15000 taatgtcatc cctactataa ctcaaatgaa tcttaagtat gccattagtg caaagaatag 15060 agctcgcacc gtagctggtg tctctatctg tagtactatg accaatagac agtttcatca 15120 aaaattattg aaatcaatag ccgccactag aggagctact gtagtaattg gaacaagcaa 15180 attctatggt ggttggcaca acatgttaaa aactgtttat agtgatgtag aaaaccctca 15240 ccttatgggt tgggattatc ctaaatgtga tagagccatg cctaacatgc ttagaattat 15300 ggcctcactt gttcttgctc gcaaacatac aacgtgttgt agcttgtcac accgtttcta 15360 tagattagct aatgagtgtg ctcaagtatt gagtgaaatg gtcatgtgtg gcggttcact 15420 atatgttaaa ccaggtggaa cctcatcagg agatgccaca actgcttatg ctaatagtgt 15480 ttttaacatt tgtcaagctg tcacggccaa tgttaatgca cttttatcta ctgatggtaa 15540 caaaattgcc gataagtatg tccgcaattt acaacacaga ctttatgagt gtctctatag 15600 aaatagagat gttgacacag actttgtgaa tgagttttac gcatatttgc gtaaacattt 15660 ctcaatgatg atactctctg acgatgctgt tgtgtgtttc aatagcactt atgcatctca 15720 aggtctagtg gctagcataa agaactttaa gtcagttctt tattatcaaa acaatgtttt 15780 tatgtctgaa gcaaaatgtt ggactgagac tgaccttact aaaggacctc atgaattttg 15840 ctctcaacat acaatgctag ttaaacaggg tgatgattat gtgtaccttc cttacccaga 15900 tccatcaaga atcctagggg ccggctgttt tgtagatgat atcgtaaaaa cagatggtac 15960 acttatgatt gaacggttcg tgtctttagc tatagatgct tacccactta ctaaacatcc 16020 taatcaggag tatgctgatg tctttcattt gtacttacaa tacataagaa agctacatga 16080 tgagttaaca ggacacatgt tagacatgta ttctgttatg cttactaatg ataacacttc 16140 aaggtattgg gaacctgagt tttatgaggc tatgtacaca ccgcatacag tcttacaggc 16200 tgttggggct tgtgttcttt gcaattcaca gacttcatta agatgtggtg cttgcatacg 16260 tagaccattc ttatgttgta aatgctgtta cgaccatgtc atatcaacat cacataaatt 16320 agtcttgtct gttaatccgt atgtttgcaa tgctccaggt tgtgatgtca cagatgtgac 16380 tcaactttac ttaggaggta tgagctatta ttgtaaatca cataaaccac ccattagttt 16440 tccattgtgt gctaatggac aagtttttgg tttatataaa aatacatgtg ttggtagcga 16500 taatgttact gactttaatg caattgcaac atgtgactgg acaaatgctg gtgattacat 16560 tttagctaac acctgtactg aaagactcaa gctttttgca gcagaaacgc tcaaagctac 16620 tgaggagaca tttaaactgt cttatggtat tgctactgta cgtgaagtgc tgtctgacag 16680 agaattacat ctttcatggg aagttggtaa acctagacca ccacttaacc gaaattatgt 16740 ctttactggt tatcgtgtaa ctaaaaacag taaagtacaa ataggagagt acacctttga 16800 aaaaggtgac tatggtgatg ctgttgttta ccgaggtaca acaacttaca aattaaatgt 16860 tggtgattat tttgtgctga catcacatac agtaatgcca ttaagtgcac ctacactagt 16920 gccacaagag cactatgtta gaattactgg cttataccca acactcaata tctcatatga 16980 gttttctagc aatgttgcaa attatcaaaa ggttggtatg caaaagtatt ctacactcca 17040 gggaccacct ggtactggta agagtcattt tgctattggc ctagctctct actacccttc 17100 tgctcgcata gtgtatacag cttgctctca tgccgctgtt gatgcactat gtgagaaggc 17160 attaaaatat ttgcctatag ataaatgtag tagaattata cctgcacgtg ctcgtgtaga 17220 gtgttttgat aaattcaaag tgaattcaac attagaacag tatgtctttt gtactgtaaa 17280 tgcattgcct gagacgacag cagatatagt tgtctttgat gaaatttcaa tggccacaaa 17340 ttatgatttg agtgttgtca atgccagatt acgtgctaag cactatgtgt acattggcga 17400 ccctgctcaa ttacctgcac cacgcacatt gctaactaag ggcacactag aaccagaata 17460 tttcaattca gtgtgtagac ttatgaaaac tataggtcca gacatgttcc tcggaacttg 17520 tcggcgttgt cctgctgaaa ttgttgacac tgtgagtgct ttggtttatg ataataagct 17580 taaagcacat aaagacaaat cagctcaatg ctttaaaatg ttttataagg gtgttatcac 17640 gcatgatgtt tcatctgcaa ttaacaggcc acaaataggc gtggtaagag aattccttac 17700 acgtaaccct gcttggagaa aagctgtctt tatttcacct tataattcac agaatgctgt 17760 agcctcaaag attttgggac taccaactca aactgttgat tcatcacagg gctcagaata 17820 tgactatgtc atattcactc aaaccactga aacagctcac tcttgtaatg taaacagatt 17880 taatgttgct attaccagag caaaagtagg catactttgc ataatgtctg atagagacct 17940 ttatgacaag ttgcaattta caagtcttga aattccacgt aggaatgtgg caactttaca 18000 agctgaaaat gtaacaggac tctttaaaga ttgtagtaag gtaatcactg ggttacatcc 18060 tacacaggca cctacacacc tcagtgttga cactaaattc aaaactgaag gtttatgtgt 18120 tgacatacct ggcataccta aggacatgac ctatagaaga ctcatctcta tgatgggttt 18180 taaaatgaat tatcaagtta atggttaccc taacatgttt atcacccgcg aagaagctat 18240 aagacatgta cgtgcatgga ttggcttcga tgtcgagggg tgtcatgcta ctagagaagc 18300 tgttggtacc aatttacctt tacagctagg tttttctaca ggtgttaacc tagttgctgt 18360 acctacaggt tatgttgata cacctaataa tacagatttt tccagagtta gtgctaaacc 18420 accgcctgga gatcaattta aacacctcat accacttatg tacaaaggac ttccttggaa 18480 tgtagtgcgt ataaagattg tacaaatgtt aagtgacaca cttaaaaatc tctctgacag 18540 agtcgtattt gtcttatggg cacatggctt tgagttgaca tctatgaagt attttgtgaa 18600 aataggacct gagcgcacct gttgtctatg tgatagacgt gccacatgct tttccactgc 18660 ttcagacact tatgcctgtt ggcatcattc tattggattt gattacgtct ataatccgtt 18720 tatgattgat gttcaacaat ggggttttac aggtaaccta caaagcaacc atgatctgta 18780 ttgtcaagtc catggtaatg cacatgtagc tagttgtgat gcaatcatga ctaggtgtct 18840 agctgtccac gagtgctttg ttaagcgtgt tgactggact attgaatatc ctataattgg 18900 tgatgaactg aagattaatg cggcttgtag aaaggttcaa cacatggttg ttaaagctgc 18960 attattagca gacaaattcc cagttcttca cgacattggt aaccctaaag ctattaagtg 19020 tgtacctcaa gctgatgtag aatggaagtt ctatgatgca cagccttgta gtgacaaagc 19080 ttataaaata gaagaattat tctattctta tgccacacat tctgacaaat tcacagatgg 19140 tgtatgccta ttttggaatt gcaatgtcga tagatatcct gctaattcca ttgtttgtag 19200 atttgacact agagtgctat ctaaccttaa cttgcctggt tgtgatggtg gcagtttgta 19260 tgtaaataaa catgcattcc acacaccagc ttttgataaa agtgcttttg ttaatttaaa 19320 acaattacca tttttctatt actctgacag tccatgtgag tctcatggaa aacaagtagt 19380 gtcagatata gattatgtac cactaaagtc tgctacgtgt ataacacgtt gcaatttagg 19440 tggtgctgtc tgtagacatc atgctaatga gtacagattg tatctcgatg cttataacat 19500 gatgatctca gctggcttta gcttgtgggt ttacaaacaa tttgatactt ataacctctg 19560 gaacactttt acaagacttc agagtttaga aaatgtggct tttaatgttg taaataaggg 19620 acactttgat ggacaacagg gtgaagtacc agtttctatc attaataaca ctgtttacac 19680 aaaagttgat ggtgttgatg tagaattgtt tgaaaataaa acaacattac ctgttaatgt 19740 agcatttgag ctttgggcta agcgcaacat taaaccagta ccagaggtga aaatactcaa 19800 taatttgggt gtggacattg ctgctaatac tgtgatctgg gactacaaaa gagatgctcc 19860 agcacatata tctactattg gtgtttgttc tatgactgac atagccaaga aaccaactga 19920 aacgatttgt gcaccactca ctgtcttttt tgatggtaga gttgatggtc aagtagactt 19980 atttagaaat gcccgtaatg gtgttcttat tacagaaggt agtgttaaag gtttacaacc 20040 atctgtaggt cccaaacaag ctagtcttaa tggagtcaca ttaattggag aagccgtaaa 20100 aacacagttc aattattata agaaagttga tggtgttgtc caacaattac ctgaaactta 20160 ctttactcag agtagaaatt tacaagaatt taaacccagg agtcaaatgg aaattgattt 20220 cttagaatta gctatggatg aattcattga acggtataaa ttagaaggct atgccttcga 20280 acatatcgtt tatggagatt ttagtcatag tcagttaggt ggtttacatc tactgattgg 20340 actagctaaa cgttttaagg aatcaccttt tgaattagaa gattttattc ctatggacag 20400 tacagttaaa aactatttca taacagatgc gcaaacaggt tcatctaagt gtgtgtgttc 20460 tgttattgat ttattacttg atgattttgt tgaaataata aaatcccaag atttatctgt 20520 agtttctaag gttgtcaaag tgactattga ctatacagaa atttcattta tgctttggtg 20580 taaagatggc catgtagaaa cattttaccc aaaattacaa tctagtcaag cgtggcaacc 20640 gggtgttgct atgcctaatc tttacaaaat gcaaagaatg ctattagaaa agtgtgacct 20700 tcaaaattat ggtgatagtg caacattacc taaaggcata atgatgaatg tcgcaaaata 20760 tactcaactg tgtcaatatt taaacacatt aacattagct gtaccctata atatgagagt 20820 tatacatttt ggtgctggtt ctgataaagg agttgcacca ggtacagctg ttttaagaca 20880 gtggttgcct acgggtacgc tgcttgtcga ttcagatctt aatgactttg tctctgatgc 20940 agattcaact ttgattggtg attgtgcaac tgtacataca gctaataaat gggatctcat 21000 tattagtgat atgtacgacc ctaagactaa aaatgttaca aaagaaaatg actctaaaga 21060 gggttttttc acttacattt gtgggtttat acaacaaaag ctagctcttg gaggttccgt 21120 ggctataaag ataacagaac attcttggaa tgctgatctt tataagctca tgggacactt 21180 cgcatggtgg acagcctttg ttactaatgt gaatgcgtca tcatctgaag catttttaat 21240 tggatgtaat tatcttggca aaccacgcga acaaatagat ggttatgtca tgcatgcaaa 21300 ttacatattt tggaggaata caaatccaat tcagttgtct tcctattctt tatttgacat 21360 gagtaaattt ccccttaaat taaggggtac tgctgttatg tctttaaaag aaggtcaaat 21420 caatgatatg attttatctc ttcttagtaa aggtagactt ataattagag aaaacaacag 21480 agttgttatt tctagtgatg ttcttgttaa caactaaacg aacaatgttt gtttttcttg 21540 ttttattgcc actagtctct attcagtgtg ttaatcttac aaccagaact caattacccc 21600 ctgcatacac taattctttc acacgtggtg tttattaccc tgacaaagtt ttcagatcct 21660 cagttttaca ttcaactcag gacttgttct tacctttctt ttccaatgtt acttggttcc 21720 atgctataca tgtctctggg accaatggta ctaagaggtt tgataaccct gtcctaccat 21780 ttaatgatgg tgtttatttt gcttccactg agaagtctaa cataataaga ggctggattt 21840 ttggtactac tttagattcg aagacccagt ccctacttat tgttaataac gctactaatg 21900 ttgttattaa agtctgtgaa tttcaatttt gtaatgatcc atttttgggt gtttattacc 21960 acaaaaacaa caaaagttgt atggaaagtg agttcagagt ttattctagt gcgaataatt 22020 gcacttttga atatgtctct cagccttttc ttatggacct tgaaggaaaa cagggtaatt 22080 tcaaaaatct tagggaattt gtgtttaaga atattgatgg ttattttaaa atatattcta 22140 agcacacgcc tattaattta gtgcgtgatc tccctcaggg tttttcggct ttagaaccat 22200 tggtagattt gccaataggt attaacatca ctaggtttca aactttactt gctttacata 22260 gaagttattt gactcctggt gattcttctt caggttggac agctggtgct gcagcttatt 22320 atgtgggtta tcttcaacct aggacttttc tattaaaata taatgaaaat ggaaccatta 22380 cagatgctgt agactgtgca cttgaccctc tctcagaaac aaagtgtacg ttgaaatcct 22440 tcactgtaga aaaaggaatc tatcaaactt ctaactttag agtccaacca acagaatcta 22500 ttgttagatt tcctaatatt acaaacttgt gcccttttgg tgaagttttt aacgccacca 22560 gatttgcatc tgtttatgct tggaacagga agagaatcag caactgtgtt gctgattatt 22620 ctgtcctata taattccgca tcattttcca cttttaagtg ttatggagtg tctcctacta 22680 aattaaatga tctctgcttt actaatgtct atgcagattc atttgtaatt agaggtgatg 22740 aagtcagaca aatcgctcca gggcaaactg gaaagattgc tgattataat tataaattac 22800 cagatgattt tacaggctgc gttatagctt ggaattctaa caatcttgat tctaaggttg 22860 gtggtaatta taattaccgg tatagattgt ttaggaagtc taatctcaaa ccttttgaga 22920 gagatatttc aactgaaatc tatcaggccg gtagcacacc ttgtaatggt gttgaaggtt 22980 ttaattgtta ctttccttta caatcatatg gtttccaacc cactaatggt gttggttacc 23040 aaccatacag agtagtagta ctttcttttg aacttctaca tgcaccagca actgtttgtg 23100 gacctaaaaa gtctactaat ttggttaaaa acaaatgtgt caatttcaac ttcaatggtt 23160 taacaggcac aggtgttctt actgagtcta acaaaaagtt tctgcctttc caacaatttg 23220 gcagagacat tgctgacact actgatgctg tccgtgatcc acagacactt gagattcttg 23280 acattacacc atgttctttt ggtggtgtca gtgttataac accaggaaca aatacttcta 23340 accaggttgc tgttctttat cagggtgtta actgcacaga agtccctgtt gctattcatg 23400 cagatcaact tactcctact tggcgtgttt attctacagg ttctaatgtt tttcaaacac 23460 gtgcaggctg tttaataggg gctgaacatg tcaacaactc atatgagtgt gacataccca 23520 ttggtgcagg tatatgcgct agttatcaga ctcagactaa ttctcctcgg cgggcacgta 23580 gtgtagctag tcaatccatc attgcctaca ctatgtcact tggtgcagaa aattcagttg 23640 cttactctaa taactctatt gccataccca caaattttac tattagtgtt accacagaaa 23700 ttctaccagt gtctatgacc aagacatcag tagattgtac aatgtacatt tgtggtgatt 23760 caactgaatg cagcaatctt ttgttgcaat atggcagttt ttgtacacaa ttaaaccgtg 23820 ctttaactgg aatagctgtt gaacaagaca aaaacaccca agaagttttt gcacaagtca 23880 aacaaattta caaaacacca ccaattaaag attttggtgg ttttaatttt tcacaaatat 23940 taccagatcc atcaaaacca agcaagaggt catttattga agatctactt ttcaacaaag 24000 tgacacttgc agatgctggc ttcatcaaac aatatggtga ttgccttggt gatattgctg 24060 ctagagacct catttgtgca caaaagttta acggccttac tgttttgcca cctttgctca 24120 cagatgaaat gattgctcaa tacacttctg cactgttagc gggtacaatc acttctggtt 24180 ggacctttgg tgcaggtgct gcattacaaa taccatttgc tatgcaaatg gcttataggt 24240 ttaatggtat tggagttaca cagaatgttc tctatgagaa ccaaaaattg attgccaacc 24300 aatttaatag cgctattggc aaaattcaag actcactttc ttccacagca agtgcacttg 24360 gaaaacttca agatgtggtc aaccaaaatg cacaagcttt aaacacgctt gttaaacaac 24420 ttagctccaa ttttggtgca atttcaagtg ttttaaatga tatcctttca cgtcttgaca 24480 aagttgaggc tgaagtgcaa attgataggt tgatcacagg cagacttcaa agtttgcaga 24540 catatgtgac tcaacaatta attagagctg cagaaatcag agcttctgct aatcttgctg 24600 ctactaaaat gtcagagtgt gtacttggac aatcaaaaag agttgatttt tgtggaaagg 24660 gctatcatct tatgtccttc cctcagtcag cacctcatgg tgtagtcttc ttgcatgtga 24720 cttatgtccc tgcacaagaa aagaacttca caactgctcc tgccatttgt catgatggaa 24780 aagcacactt tcctcgtgaa ggtgtctttg tttcaaatgg cacacactgg tttgtaacac 24840 aaaggaattt ttatgaacca caaatcatta ctacagacaa cacatttgtg tctggtaact 24900 gtgatgttgt aataggaatt gtcaacaaca cagtttatga tcctttgcaa cctgaattag 24960 actcattcaa ggaggagtta gataaatatt ttaagaatca tacatcacca gatgttgatt 25020 taggtgacat ctctggcatt aatgcttcag ttgtaaacat tcaaaaagaa attgaccgcc 25080 tcaatgaggt tgccaagaat ttaaatgaat ctctcatcga tctccaagaa cttggaaagt 25140 atgagcagta tataaaatgg ccatggtaca tttggctagg ttttatagct ggcttgattg 25200 ccatagtaat ggtgacaatt atgctttgct gtatgaccag ttgctgtagt tgtctcaagg 25260 gctgttgttc ttgtggatcc tgctgcaaat ttgatgaaga cgactctgag ccagtgctca 25320 aaggagtcaa attacattac acataaacga acttatggat ttgtttatga gaatcttcac 25380 aattggaact gtaactttga agcaaggtga aatcaaggat gctactcctt cagattttgt 25440 tcgcgctact gcaacgatac cgatacaagc ctcactccct ttcggatggc ttattgttgg 25500 cgttgcactt cttgctgttt ttcatagcgc ttccaaaatc ataaccctca aaaagagatg 25560 gcaactagca ctctccaagg gtgttcactt tgtttgcaac ttgctgttgt tgtttgtaac 25620 agtttactca caccttttgc tcgttgctgt tggccttgaa gccccttttc tctatcttta 25680 tgctttagtc tacttcttgc agagtataaa ctttgtaaga ataataatga ggctttggct 25740 ttgctggaaa tgccgttcca aaaacccatt actttatgat gccaactatt ttctttgctg 25800 gcatactaat tgttacgact attgtatacc ttacaatagt gtaacttctt caattgtcat 25860 tacttcaggt gatggcacaa caagtcctat ttctgaacat gactaccaga ttggtggtta 25920 tactgaaaaa tgggaatctg gagtaaaaga ctgtgttgta ttacacagtt acttcacttc 25980 agactattac cagctgtact caactcaatt gagtacagac actggtgttg aacatgttac 26040 cttcttcatc tacaataaaa ttgttgatga gcctgaagaa catgtccaaa ttcacacaat 26100 cgacggttca tccggagttg ttaatccagt aatggaacca atttatgatg aaccgacgac 26160 gactactagc gtgcctttgt aagcacaagc tgatgagtac gaacttatgt actcattcgt 26220 ttcggaagag acaggtacgt taatagttaa tagcgtactt ctttttcttg ctttcgtggt 26280 attcttgcta gttacactag ccatccttac tgcgcttcga ttgtgtgcgt actgctgcaa 26340 tattgttaac gtgagtcttg taaaaccttc tttttacgtt tactctcgtg ttaaaaatct 26400 gaattcttct agagttcctg atcttctggt ctaaacgaac taaatattat attagttttt 26460 ctgtttggaa ctttaatttt agccatggca gattccaacg gtactattac cgttgaagag 26520 cttaaaaagc tccttgaaca atggaaccta gtaataggtt tcctattcct tacatggatt 26580 tgtcttctac aatttgccta tgccaacagg aataggtttt tgtatataat taagttaatt 26640 tttctctggc tgttatggcc agtaacttta gcttgttttg tgcttgctgc tgtttacaga 26700 ataaattgga tcaccggtgg aattgctatc gcaatggctt gtcttgtagg cttgatgtgg 26760 ctcagctact tcattgcttc tttcagactg tttgcgcgta cgcgttccat gtggtcattc 26820 aatccagaaa ctaacattct tctcaacgtg ccactccatg gcactattct gaccagaccg 26880 cttctagaaa gtgaactcgt aatcggagct gtgatccttc gtggacatct tcgtattgct 26940 ggacaccatc taggacgctg tgacatcaag gacctgccta aagaaatcac tgttgctaca 27000 tcacgaacgc tttcttatta caaattggga gcttcgcagc gtgtagcagg tgactcaggt 27060 tttgctgcat acagtcgcta caggattggc aactataaat taaacacaga ccattccagt 27120 agcagtgaca atattgcttt gcttgtacag taagtgacaa cagatgtttc atctcgttga 27180 ctttcaggtt actatagcag agatattact aattattatg aggactttta aagtttccat 27240 ttggaatctt gattacatca taaacctcat aattaaaaat ttatctaagt cactaactga 27300 gaataaatat tctcaattag atgaagagca accaatggag attgattaaa cgaacatgaa 27360 aattattctt ttcttggcac tgataacact cgctacttgt gagctttatc actaccaaga 27420 gtgtgttaga ggtacaacag tacttttaaa agaaccttgc tcttctggaa catacgaggg 27480 caattcacca tttcatcctc tagctgataa caaatttgca ctgacttgct ttagcactca 27540 atttgctttt gcttgtcctg acggcgtaaa acacgtctat cagttacgtg ccagatcagt 27600 ttcacctaaa ctgttcatca gacaagagga agttcaagaa ctttactctc caatttttct 27660 tattgttgcg gcaatagtgt ttataacact ttgcttcaca ctcaaaagaa agacagaatg 27720 attgaacttt cattaattga cttctatttg tgctttttag cctttctgct attccttgtt 27780 ttaattatgc ttattatctt ttggttctca cttgaactgc aagatcataa tgaaacttgt 27840 cacgcctaaa ctaacatgaa atttcttgtt ttcttaggaa tcatcacaac tgtagctgca 27900 tttcaccaag aatgtagttt acagtcatgt actcaacatc aaccatatgt agttgatgac 27960 ccgtgtccta ttcacttcta ttctaaatgg tatattagag taggagctag aaaatcagca 28020 cctttaattg aattgtgcgt ggatgaggct ggttctaaat cacccattca gtacatcgat 28080 atcggtaatt atacagtttc ctgtttacct tttacaatta attgccagga acctaaattg 28140 ggtagtcttg tagtgcgttg ttcgttctat gaagactttt tagagtatca tgacgttcgt 28200 gttgttttag atttcatcta aacgaacaaa ctataatgtc tgataatgga ccccaaaatc 28260 agcgaaatgc accccgcatt acgtttggtg gaccctcaga ttcaactggc agtaaccaga 28320 atggagaacg cagtggggcg cgatcaaaac aacgtcggcc ccaaggttta cccaataata 28380 ctgcgtcttg gttcaccgct ctcactcaac atggcaagga agaccttaaa ttccctcgag 28440 gacaaggcgt tccaattaac accaatagca gtccagatga ccaaattggc tactaccgaa 28500 gagctaccag acgaattcgt ggtggtgacg gtaaaatgaa agatctcagt ccaagatggt 28560 atttctacta cctaggaact gggccagaag ctggacttcc ctatggtgct aacaaagacg 28620 gcatcatatg ggttgcaact gagggagcct tgaatacacc aaaagatcac attggcaccc 28680 gcaatcctgc taacaatgct gcaatcgtgc tacaacttcc tcaaggaaca acattgccaa 28740 aaggcttcta cgcagaaggg agcagaggcg gcagtcaagc ctcttctcgt tcctcatcac 28800 gtagtcgcaa cagttcaaga aattcaactc caggcagcag taggggaatt tctcctgcta 28860 gaatggctgg caatggcggt gatgctgctc ttgctttgct gctgcttgac agattgaacc 28920 agcttgagag caaaatgtct ggtaaaggcc aacaacaaca aggccaaact gtcactaaga 28980 aatctgctgc tgaggcttct aagaagcctc ggcaaaaacg tactgccact aaagcataca 29040 atgtaacaca agctttcggc agacgtggtc cagaacaaac ccaaggaaat tttggggacc 29100 aggaactaat cagacaagga actgattaca aacattggcc gcaaattgca caatttgccc 29160 ccagcgcttc agcgttcttc ggaatgtcgc gcattggcat ggaagtcaca ccttcgggaa 29220 cgtggttgac ctacacaggt gccatcaaat tggatgacaa agatccaaat ttcaaagatc 29280 aagtcatttt gctgaataag catattgacg catacaaaac atttccacca acagagccta 29340 aaaaggacaa aaagaagaag gctgatgaaa ctcaagcctt accgcagaga cagaagaaac 29400 agcaaactgt gactcttctt cctgctgcag atttggatga tttctccaaa caattgcaac 29460 aatccatgag cagtgctgac tcaactcagg cctaaactca tgcagaccac acaaggcaga 29520 tgggctatat aaacgttttc gcttttccgt ttacgatata tagtctactc ttgtgcagaa 29580 tgaattctcg taactacata gcacaagtag atgtagttaa ctttaatctc acatagcaat 29640 ctttaatcag tgtgtaacat tagggaggac ttgaaagagc caccacattt tcaccgaggc 29700 cacgcggagt acgatcgagt gtacagtgaa caatgctagg gagagctgcc tatatggaag 29760 agccctaatg tgtaaaatta attttagtag tgctatcccc atgtgatttt aatagc 29816 <210> 27 <211> 1273 <212> PRT <213> SARS-CoV2 <400> 27 Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ile Gln Cys Val 1 5 10 15 Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 20 25 30 Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 35 40 45 His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 50 55 60 Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 65 70 75 80 Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 85 90 95 Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 100 105 110 Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 115 120 125 Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 130 135 140 Tyr His Lys Asn Asn Lys Ser Cys Met Glu Ser Glu Phe Arg Val Tyr 145 150 155 160 Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 165 170 175 Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 180 185 190 Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 195 200 205 Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 210 215 220 Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 225 230 235 240 Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 245 250 255 Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 260 265 270 Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 275 280 285 Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 290 295 300 Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 305 310 315 320 Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 325 330 335 Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 340 345 350 Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 355 360 365 Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 370 375 380 Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 385 390 395 400 Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 405 410 415 Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 420 425 430 Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 435 440 445 Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 450 455 460 Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 465 470 475 480 Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 485 490 495 Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 500 505 510 Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 515 520 525 Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 530 535 540 Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 545 550 555 560 Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 565 570 575 Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 580 585 590 Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 595 600 605 Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile 610 615 620 His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 625 630 635 640 Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 645 650 655 Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 660 665 670 Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 675 680 685 Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 690 695 700 Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 705 710 715 720 Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 725 730 735 Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 740 745 750 Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 755 760 765 Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 770 775 780 Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 785 790 795 800 Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 805 810 815 Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 820 825 830 Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 835 840 845 Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 850 855 860 Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 865 870 875 880 Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 885 890 895 Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 900 905 910 Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 915 920 925 Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 930 935 940 Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 945 950 955 960 Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 965 970 975 Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 980 985 990 Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 995 1000 1005 Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 1010 1015 1020 Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 1025 1030 1035 1040 Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 1045 1050 1055 Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 1060 1065 1070 Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 1075 1080 1085 Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 1090 1095 1100 Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 1105 1110 1115 1120 Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 1125 1130 1135 Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 1140 1145 1150 Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 1155 1160 1165 Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 1170 1175 1180 Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 1185 1190 1195 1200 Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 1205 1210 1215 Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 1220 1225 1230 Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 1235 1240 1245 Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 1250 1255 1260 Leu Lys Gly Val Lys Leu His Tyr Thr 1265 1270 <210> 28 <211> 419 <212> PRT <213> SARS-CoV2 <400> 28 Met Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr 1 5 10 15 Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg 20 25 30 Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn 35 40 45 Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu 50 55 60 Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro 65 70 75 80 Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly 85 90 95 Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr 100 105 110 Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp 115 120 125 Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp 130 135 140 His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln 145 150 155 160 Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser 165 170 175 Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn 180 185 190 Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala 195 200 205 Arg Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu 210 215 220 Asp Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln 225 230 235 240 Gln Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys 245 250 255 Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln 260 265 270 Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp 275 280 285 Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile 290 295 300 Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile 305 310 315 320 Gly Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala 325 330 335 Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu 340 345 350 Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro 355 360 365 Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln 370 375 380 Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu 385 390 395 400 Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser 405 410 415 Thr Gln Ala <210> 29 <211> 222 <212> PRT <213> SARS-CoV2 <400> 29 Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 1 5 10 15 Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 20 25 30 Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 35 40 45 Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 50 55 60 Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 65 70 75 80 Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 85 90 95 Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 100 105 110 Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 115 120 125 Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile 130 135 140 Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 145 150 155 160 Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu 165 170 175 Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 180 185 190 Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 195 200 205 Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 210 215 220 <210> 30 <211> 10 <212> DNA <213> Artificial Sequence <220> <223> artificial sequence <400> 30 aacgttcgag 10 <210> 31 <211> 11 <212> DNA <213> Artificial Sequence <220> <223> artificial sequence <220> <221> misc_feature <222> (1)..(11) <223> n=2'-deoxy-7-deazaguanosine <400> 31 tcnaacnttc n 11 <210> 32 <211> 23 <212> DNA <213> Artificial Sequence <220> <223> artificial sequence <220> <221> misc_feature <222> (1)..(11) <223> n=2'-deoxy-7-deazaguanosine <220> <221> misc_feature <222> (12) <223> x=glycerol <220> <221> misc_feature <222> (13)..(23) <223> n=2'-deoxy-7-deazaguanosine <400> 32 tcnaacnttc nnncttncaa nct 23

Claims (71)

  1. i) 불활성화된 SARS-CoV-2 입자; ii) CpG-함유 올리고데옥시뉴클레오티드(CpG-ODN); 및 iii) 명반 어쥬번트를 포함하는, SARS-CoV-2 백신.
  2. 제1항에 있어서, 상기 CpG-ODN은 서열번호: 4에 의해 정의된 CpG 1018이고, 상기 명반 어쥬번트는 수산화알루미늄인, SARS-CoV-2 백신.
  3. 제1항 또는 제2항에 있어서, 백신 조성물에서 명반(Al3+):CpG(w/w) 비율은 약 1:10, 약 1:5, 약 1:4, 약 1:3, 약 1:2, 약 1:1, 약 2:1, 약 3:1, 약 4:1, 약 5:1, 약 10:1, 바람직하게는 약 1:3 내지 3:1, 보다 바람직하게는 약 1:2 내지 1:1인, SARS-Cov-2 백신.
  4. 제1항 내지 제3항 중 어느 한 항에 있어서, 백신 조성물에서 명반:CpG(w/w) 비율은 약 1:2인, SARS-CoV-2 백신.
  5. 제1항 내지 제4항 중 어느 한 항에 있어서, 백신의 명반 함량은 약 0.1 내지 2mg/mL, 약 0.2 내지 1.5mg/mL, 약 0.5 내지 1.3mg/mL, 특히 약 0.8 내지 1.2mg/mL, 가장 바람직하게는 약 1mg/mL, 즉, 0.5mg/용량이고, 여기서 백신은 0.5mL의 부피로 전달되는, SARS-CoV-2 백신.
  6. 제1항 내지 제5항 중 어느 한 항에 있어서, 백신의 CpG 함량은 약 0.25 내지 6mg/mL, 약 0.5 내지 3mg/mL, 약 1 내지 3mg/mL, 특히 약 1.5 내지 2.5mg/mL, 가장 바람직하게는 약 2mg/mL, 즉 1mg/용량이고, 여기서 백신은 0.5mL의 부피로 전달되는, SARS-CoV-2 백신.
  7. 제1항 내지 제6항 중 어느 한 항에 있어서, 백신에서 용량당 불활성화된 SARS-CoV-2 바이러스의 양은 SEC-HPLC에 의해 분석될 때 약 0.01 내지 25mAU(밀리-흡수 단위 x 분), 바람직하게는 약 0.05 내지 10mAU, 보다 바람직하게는 약 0.1 내지 5mAU, 가장 바람직하게는 약 0.25 내지 2.5mAU이고, 여기서 백신은 0.5mL의 부피로 전달되는, SARS-CoV-2 백신.
  8. 제1항 내지 제7항 중 어느 한 항에 있어서, 백신 조성물에서 유리(비결합) CpG의 양은 백신 조성물에서 CpG의 총량을 기준으로 10% 초과, 20% 초과, 30% 초과, 40% 초과, 50% 초과, 60% 초과, 70% 초과, 80% 초과, 90% 초과, 95% 초과, 바람직하게는 약 70% 내지 95%, 가장 바람직하게는 약 80% 내지 90%(w/w)인, SARS-CoV-2 백신.
  9. 제1항 내지 제8항 중 어느 한 항에 있어서, 백신 조성물은 적어도 하나의 완충제, 바람직하게는 포스페이트 완충제를 포함하는, SARS-CoV-2 백신.
  10. 제2항 내지 제9항 중 어느 한 항에 있어서, 수산화알루미늄을 포함하는 상기 백신은 1.25ppb 미만의 Cu를 포함하는, SARS-CoV-2 백신.
  11. 제1항 내지 제10항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자 내의 바이러스 RNA는 복제-결핍인, SARS-CoV-2 백신.
  12. 제1항 내지 제11항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자 내의 바이러스 RNA는 (i) 알킬화 및/또는 아실화되고 (ii) 하나 이상의 변형된 퓨린(바람직하게는 구아닌) 잔기 또는 가닥 절단을 포함하고/하거나 (iii) 하나 이상의 바이러스 단백질과 교차-결합되는, SARS-CoV-2 백신.
  13. 제1항 내지 제12항 중 어느 한 항에 있어서, 바이러스 입자는 바람직하게는 300 내지 700ppm, 보다 바람직하게는 500ppm의 농도로 되고 약 1 내지 48시간, 바람직하게는 20 내지 28시간, 가장 바람직하게는 24시간 동안 불활성화된, 베타-프로피오락톤-불활성화된 SARS-CoV-2 입자인, SARS-CoV-2 백신.
  14. 제1항 내지 제13항 중 어느 한 항에 있어서, 바이러스 입자는 자외선(UV)-불활성화된 SARS-CoV-2 입자인, SARS-CoV-2 백신.
  15. 제1항 내지 제14항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자에서 표면 단백질은 불활성화된 SARS-CoV-2 입자에서 바이러스 RNA에 비해 감소된 변형을 포함하고, 바람직하게는 표면 단백질은 불활성화된 SARS-CoV-2 입자에서 바이러스 RNA와 비교하여 감소된 비율의 변형된 잔기를 포함하고; 상기 변형은 천연 SARS-CoV-2 입자에 관한 것이며, 바람직하게는 상기 변형은 알킬화 및/또는 아실화된 뉴클레오티드 또는 아미노산 잔기를 포함하는, SARS-CoV-2 백신.
  16. 제1항 내지 제15항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자는 (i) 스파이크(S) 단백질; (ii) 뉴클레오캡시드(N) 단백질; (iii) 막(M) 당단백질; 및/또는 (iv) 엔벨로프(E) 단백질의 천연 형태를 포함하고; 바람직하게는 불활성화된 SARS-CoV-2 입자는 천연 형태 스파이크(S) 단백질을 포함하는, SARS-CoV-2 백신.
  17. 제1항 내지 제16항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자는 하나 이상의 베타-프로피오락톤-변형된 시스테인, 메티오닌 및/또는 히스티딘 잔기를 포함하는, SARS-CoV-2 백신.
  18. 제1항 내지 제17항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자는 200, 100, 50, 30, 20, 15, 10, 9, 8, 7 또는 6 미만의 베타-프로피오락톤-변형된 아미노산 잔기를 포함하고; 바람직하게는 불활성화된 SARS-CoV-2 입자의 스파이크(S) 단백질은 100, 50, 30, 20, 15, 10, 9, 8, 7 또는 6 미만의 베타-프로피오락톤-변형된 아미노산 잔기를 포함하고; 보다 바람직하게는 불활성화된 SARS-CoV-2 입자 또는 그의 스파이크 단백질은 20 이하의 베타-프로피오락톤-변형된 아미노산 잔기를 포함하고; 가장 바람직하게는 불활성화된 SARS-CoV-2 입자 또는 그의 스파이크 단백질은 1 내지 100, 2 내지 50, 3 내지 30, 10 내지 20 또는 약 15 베타-프로피오락톤-변형된 아미노산 잔기를 포함하는, SARS-CoV-2 백신.
  19. 제1항 내지 제18항 중 어느 한 항에 있어서, 입자 내의 SARS-CoV-2 폴리펩티드의 20%, 15%, 10%, 5% 또는 4% 미만은 베타-프로피오락톤-변형되고; 바람직하게는 입자 내의 SARS-CoV-2 폴리펩티드의 0.1 내지 10%, 보다 바람직하게는 1 내지 5%, 더욱 바람직하게는 2 내지 8% 또는 약 3-6%는, 바람직하게는 선택적으로 트립신, 키모트립신 및/또는 PNGase F를 사용한 효소적 단리 또는 산 가수분해에 이어서 질량 분광법에 의해 백신에서 검출될 때; 적어도 하나의 베타-프로피오락톤 변형을 포함하는, SARS-CoV-2 백신.
  20. 제1항 내지 제19항 중 어느 한 항에 있어서,
    (i) 불활성화된 SARS-CoV-2 입자의 스파이크(S) 단백질은 다음 잔기: 예를 들어 서열번호:3에서 49, 146, 166, 177, 207, 245, 379, 432, 519, 625, 1029, 1032, 1058, 1083, 1088, 1101, 1159 및/또는 1271; 바람직하게는 H49, H146, C166, M177, H207, H245, C432, H519, H625, M1029, H1058, H1083, H1088, H1101, H1159 및/또는 H1271; 또는 H207, H245, C379, M1029 및/또는 C1032, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치 중 하나 이상에서 베타-프로피오락톤 변형을 포함하고/하거나;
    (ii) 불활성화된 SARS-CoV-2 입자의 막(M) 당단백질은 다음 잔기: 예를 들어 서열번호: 29에서 125, 154, 155, 159 및/또는 210; 바람직하게는 H154, H155, C159 및/또는 H210 중 하나 이상에서 베타-프로피오락톤 변형을 포함하고;
    (iii) 불활성화된 SARS-CoV-2 입자의 뉴클레오캡시드(N) 단백질은, 예를 들어 서열번호: 28에서 M234에서 베타-프로피오락톤 변형을 포함하는, SARS-CoV-2 백신.
  21. 제1항 내지 제20항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자에서 30%, 20%, 10%, 5%, 3% 또는 1% 미만의 다음 잔기 중 하나 이상, 바람직하게는 다음 잔기 중 적어도 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 또는 모두는 베타-프로피오락톤 변형된, SARS-CoV-2 백신:
    (i) 스파이크(S) 단백질에서, 예를 들어 서열번호: 3에서 잔기 49, 146, 166, 177, 207, 245, 379, 432, 519, 625, 1029, 1032, 1058, 1083, 1088, 1101, 1159, 및/또는 1271; 바람직하게는 H49, H146, C166, M177, H207, H245, C432, H519, H625, M1029, H1058, H1083, H1088, H1101, H1159 및/또는 H1271; 또는 H207, H245, C379, M1029 및/또는 C1032, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치; 및/또는 (ii) 막(M) 당단백질에서, 예를 들어 서열번호: 29에서 잔기 125, 154, 155, 159 및/또는 210; 바람직하게는 H154, H155, C159 및/또는 H210; 및/또는 (iii) 예를 들어 서열번호: 28에서 뉴클레오캡시드(N) 단백질의 M234.
  22. 제1항 내지 제21항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자에서 다음의 위치 각각에서 베타-프로피오락톤-변형된 잔기의 비율은:
    (i) 스파이크(S) 단백질(예를 들어, 서열번호: 3, 또는 서열번호: 19, 21, 23, 25 또는 27에서 상응하는 위치)에서:
    (a) 잔기 H49, H146, C166, H207, H519, M1029, H1083, H1088, H1101, H1159 및/또는 H1271: 20% 미만, 바람직하게는 0.01 내지 10%, 더욱 바람직하게는 0.1 내지 5%; 및/또는
    (b) 잔기 M177, C432, H625: 30% 미만, 바람직하게는 0.1 내지 20%, 더욱 바람직하게는 1 내지 10%; 및/또는
    (c) 잔기 H245, H1058: 30% 미만, 바람직하게는 0.1 내지 20%, 더욱 바람직하게는 5 내지 15%;
    (ii) 막(M) 당단백질(예를 들어 서열번호: 29)에서:
    (f) H154: 5% 미만, 1% 미만 또는 0.1% 미만; 및/또는
    (g) H155: 10% 미만, 바람직하게는 0.1 내지 5%; 및/또는
    (h) C159: 5% 미만, 1% 미만 또는 0.1% 미만; 및/또는
    (i) H210: 20% 미만, 바람직하게는 0.1 내지 10%; 및/또는
    (iii) 뉴클레오캡시드(N) 단백질(예를 들어 서열번호: 28)에서:
    (j) M234: 90% 미만, 10% 미만 또는 0.1% 미만인, SARS-CoV-2 백신.
  23. 제1항 내지 제22항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자에 의한 포유동물 세포의 감염성은 천연 SARS-CoV-2 입자와 비교하여 적어도 99%, 99.99% 또는 99.9999% 감소되거나, 또는 불활성화된 SARS-CoV-2 입자에 의한 포유동물 세포의 감염성은 검출가능하지 않은, SARS-CoV-2 백신.
  24. 제1항 내지 제23항 중 어느 한 항에 있어서, 하나 이상의 약학적으로 허용가능한 부형제를 추가로 포함하는, SARS-CoV-2 백신.
  25. 제1항 내지 제24항 중 어느 한 항에 있어서, SARS-CoV-2 입자는 (i) 서열번호: 9에 의해 정의된 바와 같은; 또는 (ii) 서열번호: 9에 대해 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 DNA 서열에 상응하는 RNA 서열(및/또는 이의 단편, 선택적으로 변형된(바람직하게는 알킬화된 또는 아실화된) 뉴클레오티드 잔기를 포함함)을 포함하고; 바람직하게 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자는 독성 SARS-CoV-2를 패킹할 수 있는, SARS-CoV-2 백신.
  26. 제1항 내지 제25항 중 어느 한 항에 있어서, 상기 백신은 (i) 서열번호: 18에 의해 정의된 바와 같은; 또는 (ii) 서열번호: 18에 대해 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 DNA 서열에 상응하는 RNA 서열(및/또는 이의 단편, 선택적으로 변형된(바람직하게는 알킬화된 또는 아실화된) 뉴클레오티드 잔기를 포함함)을 포함하는 부가의 SARS-CoV-2 입자를 포함하고; 바람직하게 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자는 독성 SARS-CoV-2를 패킹할 수 있는, SARS-CoV-2 백신.
  27. 제1항 내지 제26항 중 어느 한 항에 있어서, 상기 백신은 (i) 서열번호: 22에 의해 정의된 바와 같은; 또는 (ii) 서열번호: 22에 대해 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 DNA 서열에 상응하는 RNA 서열(및/또는 이의 단편, 선택적으로 변형된(바람직하게는 알킬화된 또는 아실화된) 뉴클레오티드 잔기를 포함함)을 포함하는 부가의 SARS-CoV-2 입자를 포함하고; 바람직하게 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자는 독성 SARS-CoV-2를 패킹할 수 있는, SARS-CoV-2 백신.
  28. 제1항 내지 제27항 중 어느 한 항에 있어서, CpG ODN은 서열번호: 4에 의해 정의된 바와 같은 서열 5' TGACTGTGAACGTTCGAGATGA 3'을 포함하는 CpG 1018인, SARS-CoV-2 백신.
  29. 제1항 내지 제28항 중 어느 한 항에 있어서, 명반 어쥬번트는 수산화알루미늄을 포함하는, SARS-CoV-2 백신.
  30. 제1항 내지 제29항 중 어느 한 항에 있어서, 백신은 SARS-CoV 백신이 투여된 대상체를 적어도 70% 확률로 혈청전환시킬 수 있는, SARS-CoV-2 백신.
  31. 제1항 내지 제30항 중 어느 한 항에 있어서, 백신은 SARS-CoV 백신이 투여된 대상체를 적어도 90% 확률로 혈청전환시킬 수 있는, SARS-CoV-2 백신.
  32. 제30항에 있어서, SARS-CoV-2 백신은 SARS-CoV-2 백신이 투여된 대상체를 적어도 80%, 85%, 90%, 또는 95% 확률로 혈청전환시킬 수 있는, SARS-CoV-2 백신.
  33. 제1항 내지 제32항 중 어느 한 항에 있어서, 백신은 Vero 세포로부터 수득되거나 수득가능한, SARS-CoV-2 백신.
  34. 제1항 내지 제33항 중 어느 한 항에 있어서, 인간 대상체에 투여시 백신은 (i) SARS-CoV-2-연관된 질환(COVID-19)의 항체-의존성 증강(ADE)을 유도하지 않고/않거나 (ii) 대상체에서 면역병리를 유도하지 않는, SARS-CoV-2 백신.
  35. 예방적 또는 치료적으로 유효한 양의 제1항 내지 제34항 중 어느 한 항의 SARS-CoV-2 백신을 대상체에게 투여하는 것을 포함하는, SARS-CoV-2 감염 및/또는 SARS-CoV-2-연관된 질환(COVID-19)의 치료를 필요로 하는 인간 대상체에서 SARS-CoV-2 감염 및/또는 SARS-CoV-2-연관된 질환(COVID-19)을 예방 또는 치료하는 방법.
  36. 제35항에 있어서, 예방적 또는 치료적으로 유효한 양의 SARS-CoV-2 백신의 제2 용량을 투여하는 것을 추가로 포함하고, 바람직하게는 백신의 제2 용량은 제1 용량과 동일한 제형인, 방법.
  37. 제35항 또는 제36항에 있어서, 용량당 SARS-CoV-2 백신의 상기 예방적 또는 치료적으로 유효한 양은 ELISA에 의해 평가시 약 1 내지 100AU/용량, 바람직하게는 약 2 내지 75AU/용량, 바람직하게는 약 3 내지 60AU/용량, 보다 바람직하게는 약 3 내지 55AU/용량, 더욱 바람직하게는 약 3 내지 53AU/용량, 훨씬 더 바람직하게는 약 3 내지 40AU/용량, 더욱 바람직하게는 약 10 내지 60AU/용량, 20 내지 50AU/용량, 25 내지 45AU/용량 또는 30 내지 40AU/용량 예컨대 예를 들어 35AU/용량 또는 40AU/용량으로 정의되는, 방법.
  38. 제35항 내지 제37항 중 어느 한 항에 있어서, 용량당 SARS-CoV-2 백신의 상기 예방적 또는 치료적으로 유효한 양은, 예를 들어 (μ)BCA에 의해 측정시 약 0.05 내지 50μg 총 단백질, 약 0.1 내지 25μg, 약 0.25 내지 12.5μg, 바람직하게는 약 0.5 내지 5μg 총 단백질, 보다 바람직하게는 적어도 2.5μg 총 단백질, 적어도 3.5μg 총 단백질 또는 적어도 2.5μg 총 단백질, 훨씬 더 바람직하게는 2.5μg 내지 25μg, 3.5μg 내지 10μg 또는 4μg 내지 6μg 총 단백질/용량, 가장 바람직하게는 약 5μg 총 단백질/용량으로 정의되는, 방법.
  39. 제35항 내지 제38항 중 어느 한 항에 있어서, SARS-CoV-2 백신의 상기 예방적 또는 치료적으로 유효한 양은 ELISA에 의해 측정시, 약 0.025 내지 25μg S-단백질, 약 0.05 내지 12.5μg, 약 0.125 내지 6.25μg, 바람직하게는 약 0.25 내지 2.5μg S-단백질로 정의되는, 방법.
  40. 제35항 내지 제39항 중 어느 한 항에 있어서, SARS-CoV-2 백신의 2차 용량은 SARS-CoV-2 백신의 1차 용량 후 약 7일, 약 14일, 약 21일 또는 약 28일, 바람직하게는 약 21일에 투여되는, 방법.
  41. 제35항 내지 제40항 중 어느 한 항에 있어서, 투여는 SARS-CoV-2 중화 항체의 생산을 초래하는, 방법.
  42. SARS-CoV-2 백신을 생산하는 방법으로서,
    (a) 천연 SARS-CoV-2 입자를 생성하는 단계;
    (b) 천연 SARS-CoV-2 입자를 불활성화하여, 불활성화된 SARS-CoV-2 입자를 얻는 단계;
    (c) 불활성화된 SARS-CoV-2 입자를 CpG-함유 올리고데옥시뉴클레오티드(CpG ODN) 및 명반 어쥬번트를 포함하는 백신 조성물에 혼입시키는 단계를 포함하는, 방법.
  43. 제42항에 있어서, CpG-ODN은 CpG 1018이고 명반 어쥬번트는 수산화알루미늄인, 방법.
  44. 제43항에 있어서, 수산화알루미늄을 포함하는 SARS-CoV-2 백신은 1.25ppb 미만의 Cu를 함유하는, 방법.
  45. 제42항 내지 제44항 중 어느 한 항에 있어서, 백신은 인간 대상체에서 천연 SARS-CoV-2 입자에 대한 중화 항체를 생성할 수 있도록 SARS-CoV-2 입자의 천연 표면 형태가 불활성화 단계에서 보존되는, 방법.
  46. 제45항에 있어서, 불활성화 단계는 불활성화된 SARS-CoV-2 입자에서 바이러스 RNA를 우선적으로 표적화하는, 방법.
  47. 제42항 내지 제4046항 중 어느 한 항에 있어서, 불활성화 단계는 (i) 바이러스 RNA를 알킬화 및/또는 아실화하는 단계 (ii) 바이러스 RNA 안으로 퓨린(바람직하게는 구아닌) 잔기를 변형시키거나 가닥 절단을 도입하는 단계 및/또는 (iii) 바이러스 RNA를 하나 이상의 바이러스 단백질과 교차-결합하는 단계를 포함하는, 방법.
  48. 제42항 내지 제47항 중 어느 한 항에 있어서, 불활성화 단계는 천연 SARS-CoV-2 입자를 베타-프로피오락톤으로 처리하는 단계를 포함하는, 방법.
  49. 제48항에 있어서, 불활성화 단계에서 베타-프로피오락톤의 농도는, 선택적으로 35℃ 내지 39℃, 바람직하게는 약 37℃에서 2.5시간 ± 0.5시간 동안 가수분해에 이어서, 적어도 5시간, 적어도 10시간, 적어도 24시간 동안 2℃ 내지 8℃에서, 0.01 내지 1 중량%, 바람직하게는 0.05 내지 0.5 중량%, 보다 바람직하게는 약 0.1 중량%, 휠씬 더 바람직하게는 300 내지 700ppm, 가장 바람직하게는 약 500ppm인, 방법.
  50. 제48항 또는 제49항에 있어서, 천연 SARS-CoV-2 입자는 적어도 5시간, 적어도 10시간, 적어도 24시간 동안 베타-프로피오락톤과 접촉되는, 방법.
  51. 제42항 내지 제50항 중 어느 한 항에 있어서, 불활성화 단계는 약 0℃ 내지 약 25℃, 바람직하게는 약 4℃ 또는 약 8℃에서 수행되는, 방법.
  52. 제42항 내지 제51항 중 어느 한 항에 있어서, 불활성화 단계는 천연 SARS-CoV-2 입자를 자외선(UV) 광으로 처리하는 것을 포함하는, 방법.
  53. 제42항 내지 제52항 중 어느 한 항에 있어서, 단계 (a)는 다음 단계 중 하나 이상을 포함하는, 방법:
    (i) Vero 세포 상에 SARS-CoV-2를 계대하고, 이에 의해 SARS-CoV-2를 포함하는 배양 배지를 생산하는 단계;
    (ii) (i)의 배양 배지를 수확하는 단계;
    (iii) (ii)의 수확된 배양 배지를 침전시키고, 이에 의해 상등액에서 천연 SARS-CoV-2 입자를 생성하는 단계.
  54. 제53항에 있어서, 단계 (iii) 이전에 단계 (ii)의 배양 배지를 농축하는 단계를 추가로 포함하는, 방법.
  55. 제53항 또는 제54항에 있어서, 침전 단계 (iii)은 단계 (ii)의 배양 배지를 프로타민 설페이트 및/또는 벤조나아제와 접촉시키는 단계를 포함하는, 방법.
  56. 제42항 내지 제55항 중 어느 한 항에 있어서, 불활성화된 SARS-CoV-2 입자를 투석하고, 이에 의해 투석된 SARS-CoV-2를 생성하는 단계를 추가로 포함하는, 방법.
  57. 제56항에 있어서, 투석된 SARS-CoV-2를 여과하는 단계를 추가로 포함하는, 방법.
  58. 제42항 내지 제57항 중 어느 한 항에 있어서, 불활성화 단계는 천연 SARS-CoV-2 입자를 포함하는 액체 조성물을 용기에서 화학적 바이러스 불활성화제와 접촉시키는 단계, 난류가 아닌 층류의 조건 하에서 SARS-CoV-2 입자를 포함하는 액체 조성물과 화학적 바이러스 불활성화제를 혼합시키는 단계, 및 바이러스 입자를 불활성화시키기에 충분한 시간 동안 SARS-CoV-2 입자를 포함하는 액체 조성물과 화학적 바이러스 불활성화제를 인큐베이션하는 단계를 포함하는, 방법.
  59. 제58항에 있어서, 불활성화 단계는 가요성 생물반응기 백에서 수행되는, 방법.
  60. 제58항 또는 제59항에 있어서, 불활성화 단계는 불활성화의 기간 동안 5회 이하의 용기 반전을 포함하는, 방법.
  61. 제58항 내지 제60항 중 어느 한 항에 있어서, 화학적 바이러스 불활성화제와 천연 SARS-CoV-2 입자를 포함하는 조성물의 혼합은 인큐베이션 기간 동안 10rpm 이하에서 10분 이하 동안 용기를 흔들기(rocking), 회전(rotation), 궤도 진탕(orbital shaking) 또는 진동(oscillation)시키는 것을 포함하는, 방법.
  62. 제42항 내지 제61항 중 어느 한 항에 있어서, (i) 배치 크로마토그래피 및/또는 (ii) 수크로스 밀도 구배 원심분리로부터 선택되는 하나 이상의 방법에 의해 불활성화된 SARS-CoV-2 입자를 정제하는 것을 추가로 포함하는, 방법.
  63. 제42항 내지 제62항 중 어느 한 항에 있어서, 상기 SARS-CoV-2 입자는 (i) 서열번호: 9에 의해 정의된 바와 같은; 또는 (ii) 서열번호: 9에 대해 적어도 80%, 적어도 85%, 적어도 90%, 적어도 95% 또는 적어도 99% 서열 동일성을 갖는 DNA 서열에 상응하는 RNA 서열을 포함하고; 바람직하게 RNA 서열을 포함하는 천연(비-불활성화된) SARS-CoV-2 입자는 독성 SARS-CoV-2를 패킹할 수 있는, 방법.
  64. 제42항 내지 제63항 중 어느 한 항에 있어서, 상기 SARS-CoV-2 바이러스는 (i) 서열번호: 11에 의해 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 11에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성되는 S 단백질을 포함하는, 방법.
  65. 제42항 내지 제64항 중 어느 한 항에 있어서, 상기 SARS-CoV-2 바이러스는 (i) 서열번호: 10에 의해 정의된 바와 같은 아미노산 서열, 또는 (ii) 서열번호: 10에 대해 적어도 95%, 적어도 97% 또는 적어도 99% 동일성을 갖는 아미노산 서열을 포함하거나 이로 구성되는 다단백질을 포함하는, 방법.
  66. 제42항 내지 제65항 중 어느 한 항의 방법에 의해 수득되거나 수득가능한 SARS-CoV-2 백신.
  67. 대상체에서 SARS-CoV-2 감염의 치료 또는 예방을 위한 제1항 내지 제34항 또는 제66항 중 어느 한 항의 SARS-CoV-2 백신의 용도.
  68. 의약으로서 사용하기 위한 제1항 내지 제34항 또는 제66항 중 어느 한 항의 SARS-CoV-2 백신.
  69. 대상체에서 SARS-CoV-2 감염의 예방 또는 치료에 사용하기 위한 약학적 조성물로서, 상기 약학적 조성물은 선택적으로 하나 이상의 약학적으로 허용가능한 부형제와 조합한 제1항 내지 제34항 또는 제66항 중 어느 한 항의 백신인, 약학적 조성물.
  70. 제1항 내지 제69항 중 어느 한 항에 있어서, 대상체는 (i) 고령 대상체, 바람직하게는 65세 이상, 70세 이상 또는 80세 이상 연령의 대상체; (ii) 면역저하 대상체; 또는 (iii) 임신한 대상체인, 백신, 방법, 용도 또는 약학적 조성물.
  71. (i) SARS-CoV-2-연관된 질환(COVID-19)의 항체의존 면역증강(ADE); 및/또는 (ii) 대상체에서 면역병리학의 유도 없이 SARS-CoV-2 감염의 예방 또는 치료에 사용하기 위한, 제1항 내지 제70항 중 어느 한 항에 따른 백신, 방법, 용도 또는 약학적 조성물.
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