CN115337216B

CN115337216B - Skin care product combination with relieving effect and preparation method thereof

Info

Publication number: CN115337216B
Application number: CN202210902770.XA
Authority: CN
Inventors: 陈真; 马任钟; 赵波; 赵琼; 夏金凤; 陈影; 陆地
Original assignee: Yunnan Qumei Biotechnology Co ltd; Kunming Medical University
Current assignee: Yunnan Qumei Biotechnology Co ltd; Kunming Medical University
Priority date: 2022-07-28
Filing date: 2022-07-28
Publication date: 2024-02-02
Anticipated expiration: 2042-07-28
Also published as: CN115337216A

Abstract

A product combination suitable for daily skin care of sensitive skin people and a preparation method thereof comprise facial cleanser with cleaning effect, moisturizing lotion with relieving effect and repairing cream with relieving and moisturizing effects. The formula matrix of the compound fertilizer is prepared from mild and non-irritating raw materials as far as possible, so that the use of essence and spice and preservatives, surfactants and cosolvents with high irritation is avoided. For the raw materials of natural product sources, the extract with controllable components and higher purity is selected for self-pre-preparation, so that the use of finished raw materials containing a stimulating preservative and a cosolvent is avoided, the burden on the skin is reduced as much as possible, and the natural product source is suitable for sensitive skin crowds as daily skin care products and is used for a long time.

Description

Skin care product combination with relieving effect and preparation method thereof

Technical Field

The invention relates to the field of cosmetics, and relates to a skin care product combination with a soothing effect for sensitive skin groups.

Background

Sensitive Skin (SS) is a skin hyperreactive condition or poor tolerance to stimulus, and can occur in physiological or pathological conditions, and the symptoms are mainly subjective symptoms such as burning, stinging, itching and tightness of the skin when stimulated by physical, chemical, mental and other factors, and may be accompanied by objective signs such as erythema, scales and telangiectasia. In women 15.93-23% of the country, men 8.62% of the country, the actual occurrence may be higher than the report because the investigation is limited by area and number of people. The incidence of sensitive skin is high worldwide, and 60-70% of women and 50-60% of men are reported to have different degrees of skin sensitivity abroad. The current research considers that the occurrence of SS is a complex process related to skin barrier-neurovascular-immune inflammation, but there is no gold standard for SS treatment at home and abroad. The application guidelines of the skin care products of the comfort and moisture preservation type in sensitive skin, which are issued in 2017 and 2019, respectively, are all mentioned, and the daily skin care products of sensitive skin people are selected according to the following principles: (1) the cosmetic ingredients should be mild and have low irritation; (2) On the basis of meeting the normal cleaning and moisturizing functions, the skin care product has the effects of relieving (helping to improve the states of skin irritation and the like) and repairing (helping to maintain the skin in a normal state).

Improper daily skin care is a major contributor to SS, including cosmetic abuse, excessive cleaning, long-term exposure to irritating components in cosmetics, skin damage after medical treatment. Some substances which form exogenous irritation to skin can exist in the components of the conventional cosmetic formula, and the irritation can be from preservatives (such as phenoxyethanol and potassium sorbate), surfactants (such as dodecylbenzene sulfonate), cosolvents (such as polyethylene glycol and propylene glycol), essence and perfume and the like in the formula; on the other hand, when using some natural product extracts of plant or animal origin with a mature supply system as raw materials for cosmetics, it is also possible to introduce preservatives, cosolvents contained therein into the cosmetics, causing the repeated addition of these potential irritants. Although the use limit of the components is clearly defined in cosmetic safety technical Specification, the components are not at risk to healthy skin, but are still exogenous stimulation risk substances for the long-term use of SS people.

Disclosure of Invention

The invention provides a product combination suitable for daily skin care of SS people and a preparation method thereof, and the product combination comprises a facial cleanser with cleaning effect, a moisturizing lotion with relieving effect and a repairing cream with relieving and moisturizing effects. The formula matrix of the compound fertilizer is prepared from mild and non-irritating raw materials as far as possible, so that the use of essence and spice and preservatives, surfactants and cosolvents with high irritation is avoided. For the raw materials of natural product sources, the extract with controllable components and higher purity is selected for self-pre-preparation, so that the use of finished raw materials containing a stimulating preservative and a cosolvent is avoided, the burden on the skin is reduced as much as possible, and the natural product source is suitable for SS people to be used as daily skin care products for a long time.

The invention relates to a facial cleanser, which takes a proper amount of sodium cocoyl glycinate as a surfactant; proper amount of glycerol and hydroxypropyl trimethyl ammonium chloride hyaluronic acid are used as humectant; a small amount of purslane extract is used as an active substance with a relieving effect; a trace amount of octanoyl hydroxamic acid is used as a preservative. The facial cleanser has no irritation to skin and eyes, and has the advantages of moistening skin and no tightness after cleaning.

The moisturizing lotion takes a proper amount of sodium hyaluronate, trehalose, beta-glucan and chitosan as moisturizing agents; a small amount of notoginseng extract, purslane extract and lactobacillus/soybean milk fermentation product are used as the active substances with relieving efficacy; a small amount of tetrahydromethylpyrimidine carboxylic acid and bletilla tuber extract are used as repairing efficacy active substances; a trace amount of octanoyl hydroxamic acid is used as a preservative. The moisturizing lotion has no irritation to skin and eyes, and has remarkable effects of relieving allergy, resisting inflammation and moisturizing.

The repairing cream provided by the invention takes a proper amount of sodium hyaluronate, trehalose, beta-glucan, shea butter, jojoba oil and squalane as a humectant; a small amount of pseudo-ginseng extract, purslane extract and prinsepia utilis royle oil are used as the active substances with the relieving efficacy; a small amount of bletilla striata extract and ceramide are used as repairing efficacy active substances; a trace amount of octanoyl hydroxamic acid is used as a preservative. The moisturizing cream has no irritation to skin and eyes, and has remarkable effects of relieving allergy, resisting inflammation, repairing skin barrier and moisturizing.

The facial cleanser, the moisturizing lotion and the repair cream can be used independently or in combination. The combination of the two components can produce better effect than single use, and can be used for sensitive skin groups so as to relieve skin problems.

In addition, the bletilla striata is derived from the dry tuber of bletilla striata of orchidaceae, has the effects of astringing to stop bleeding, reducing swelling and promoting granulation as a traditional Chinese medicine, and has a long application history. The rhizoma bletilla polysaccharide (Bletilla Striata Polysaccharide, BSP) is main active component of rhizoma bletilla extract, and the BSP is rhizoma bletilla mannan composed of 4 molecules of mannose and 1 molecule of glucose. BSP is an excellent cosmetic raw material, and has four aspects of safety, moisture retention, film forming property and repairing activity: (1) The bletilla striata gum is a traditional Chinese medicinal material which can be eaten and externally applied, and has long use history. As a natural polymer material, BSP has been widely studied and reported to be free of irritation and adverse reaction; (2) The moisturizing property, namely the BSP can absorb water molecules with the volume 200 times of that of the BSP to form a viscous solution, and a layer of moisturizing film is formed on the surface of the skin to provide lasting moisturizing capability for the skin; (3) The film forming property and the film formed on the skin surface by the BSP can increase the residence time of other active ingredients in the cosmetics on the skin surface and enhance the action time and effect of the active substances on the skin; (4) The repair activity, BSP can activate a plurality of cytokines in the tissue repair process, and plays a role in repairing skin injury by inducing cell proliferation and cell migration. BSP activates macrophages, causing a cascade of reactions that first cause activation of fibroblasts, producing epidermal growth factor (ECGF) and Angiogenic Factor (AF) on aging wrinkled skin. ECGF promotes the production of collagen and elastin, thereby improving the appearance of skin and eliminating wrinkles. The BSP content of the bletilla striata extract used in the invention is >70%.

In order to achieve the above purpose, the invention is realized by the following technical scheme:

specifically, the facial cleanser with the effects of cleaning and moisturizing is provided, and comprises water, EDTA disodium, a surfactant, a humectant, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, a preservative, a purslane extract pre-prepared liquid and PEG-7 glycerol cocoate.

A face-cleaning milk with cleaning and moisturizing functions contains (by weight) water 45-65%, EDTA disodium 0.05-0.2%, surfactant 12-18%, humectant 15-28%, polyquaternium-100.1-0.3%, sodium methyl cocoyl taurate 2-4%, sodium lauroyl isethionate 3-5%, citric acid 0.9-1.3%, preservative 1.0-1.4%, herba Portulacae extract pre-prepared liquid 0.5-1.5%, PEG-7 glycerin cocoate 1.0-2.5%.

Further, the surfactant is sodium cocoyl glycinate.

The humectant is glycerin and hydroxypropyl trimethyl ammonium chloride hyaluronic acid.

A face-cleaning milk with cleaning and moisturizing functions contains (by weight) water 45-65%, EDTA disodium 0.05-0.2%, sodium cocoyl glycinate 12-18%, glycerin 15-25%, polyquaternium-100.1-0.3%, sodium methyl cocoyl taurate 2-4%, sodium lauroyl isethionate 3-5%, citric acid 0.9-1.3%, hydroxypropyl trimethylammonium chloride hyaluronic acid 0.05-0.15%, preservative 1.0-1.4%, herba Portulacae extract pre-prepared solution 0.5-1.5% and PEG-7 glycerin cocoate 1.0-2.5%.

Further, the purslane extract is an extract of purslane (Port M ca OLERACEA).

The purslane extract pre-prepared liquid is prepared by diluting purslane concentrated liquid for extracting effective matters with 1, 3-propanediol of biological origin and water to a certain concentration. The 1, 3-propanediol is a biological source, corn is obtained through biological fermentation, the purity of the corn can reach 99.8 percent, and the corn is a pure natural humectant with antiseptic efficacy, and has no irritation or sensitization to skin.

The weight ratio of the purslane concentrated solution to the 1, 3-propanediol to the water is as follows: 0.2 to 0.8:25 to 35:60 to 80, preferably 0.4:30:69.6.

The preservative is PHL and consists of octanoyl hydroxamic acid (CAPRYLHYDROXAMIC ACID), 1, 2-hexanediol and 1, 3-propanediol.

Preferably, the weight ratio of octanoyl hydroxamic acid (CAPRYLHYDROXAMIC ACID), 1, 2-hexanediol and 1, 3-propanediol is: 1-8:20-40:50-70, preferably 5:30:65.

The PHL is prepared by adding octanoyl hydroxamic acid into 1, 3-propanediol and 1, 2-hexanediol, heating to 45-55deg.C, stirring to dissolve completely, and cooling to room temperature for use.

The EDTA disodium, sodium cocoyl glycinate, glycerol, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, hydroxypropyl trimethyl ammonium chloride hyaluronic acid and PEG-7 glycerol cocoate can be purchased from a non-standard cosmetic raw material supplier.

The 1, 3-propylene glycol is 100% corn source, contains no petroleum and animal raw materials, has high purity (up to 99.8%), has no irritation or sensitization to skin, and has antiseptic and moisturizing effects. 1, 3-propanediol is a better and milder alternative to co-solvents than 1, 2-propanediol and butanediol, which are petroleum sources.

The water is deionized water.

Further, there is provided: a facial cleanser with cleaning and moisturizing effects comprises 59.7kg of water, 12kg of sodium cocoyl glycinate, 18kg of glycerin, 100.1kg of polyquaternium, 0.1kg of disodium EDTA, 2.4kg of sodium methyl cocoyl taurate, 3kg of sodium lauroyl isethionate, 1.1kg of citric acid, 0.1kg of hydroxypropyl trimethylammonium chloride hyaluronic acid, 1.2kg of PHL, 0.8kg of purslane extract pre-prepared liquid and 1.5kg of PEG-7 glycerin cocoate.

The preparation method of the facial cleanser with the effects of cleaning and moisturizing comprises the following steps of:

(1) Adding raw material water, polyquaternium-10, sodium cocoyl glycinate, glycerol, sodium methyl cocoyl taurate and sodium lauroyl isethionate into an emulsifying pot, stirring and heating, adding raw material citric acid and EDTA disodium, stirring and dissolving completely.

(2) And (3) cooling: stopping heating, and continuously stirring and cooling.

(3) And (3) completely dissolving the raw material hydroxypropyl trimethyl ammonium chloride hyaluronic acid with a proper amount of water, adding the mixture into an emulsifying pot, and uniformly stirring and mixing the mixture.

(4) Adding PHL and herba Portulacae extract pre-mixed solution and PEG-7 glycerol cocoate into emulsifying pot, stirring, and mixing.

Wherein the condition of the step (1) is that the stirring time is 40-60 minutes, the temperature is 75-85 ℃, the vacuum degree is-0.01 to-0.03 MPa, and the preferable condition is that the stirring time is 50 minutes, the temperature is 80 ℃, and the vacuum is-0.02 MPa.

The condition of the step (2) is that the stirring time is 40-60 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.01 to-0.03 MPa, the preferable condition is that the stirring time is 55 minutes, the temperature is 38 ℃, and the vacuum is-0.02 MPa.

The condition of the step (3) is that the stirring time is 10-15 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.01 to-0.03 MPa, and the preferable condition is that the stirring time is 10 minutes, the temperature is 37 ℃, and the vacuum is-0.02 MPa.

The condition of the step (4) is that the stirring time is 10-15 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.01 to-0.03 MPa, and the preferable condition is that the stirring time is 15 minutes, the temperature is 37 ℃, and the vacuum is-0.02 MPa.

Specifically, the moisturizing lotion with the relieving effect comprises water, glycerol, butanediol, sodium hyaluronate, betaine, EDTA disodium, panthenol, dipotassium glycyrrhizinate, beta-glucan, chitosan, pseudo-ginseng extract pre-prepared liquid, purslane extract pre-prepared liquid, lactobacillus/soybean milk fermentation products, tetrahydropyrimidine carboxylic acid, bletilla striata extract, a preservative and polyacrylate crosslinked polymer-6.

A moisturizing lotion with relieving effect comprises, by weight, 80-90% of water, 3-5% of glycerol, 2-5% of butanediol, 0.12-0.24% of sodium hyaluronate, 1-3% of betaine, 0.05-0.2% of EDTA disodium, 0.1-0.5% of panthenol, 0.05-0.1% of dipotassium glycyrrhizinate, 0.02-0.05% of beta-glucan, 0.5-2% of chitosan, 2-5% of pseudo-ginseng extract pre-preparation liquid, 0.5-2% of purslane extract pre-preparation liquid, 0.5-2% of lactobacillus/soybean milk fermentation product, 0.1-0.5% of tetrahydropyrimidine carboxylic acid, 0.2-2% of bletilla tuber extract, 1.0-1.4% of preservative and 60.3-0.7% of polyacrylate crosslinked polymer.

Wherein sodium hyaluronate, betaine, beta-glucan and chitosan are used as humectant.

Wherein Notoginseng radix extract, herba Portulacae extract, lactobacillus/soybean milk fermentation product is active substance with relieving effect.

Wherein, the tetrahydromethylpyrimidine carboxylic acid and the bletilla tuber extract are active matters with repairing efficacy.

Wherein the preservative is PHL.

The sodium hyaluronate is HA (molecular weight 10 ⁶ Da) and oligosaccharide HA (molecular weight 10 ⁴ Da) in a 3:2 ratio.

The pseudo-ginseng extract pre-prepared liquid is prepared by fermenting pseudo-ginseng extract by microorganisms, separating and freeze-drying effective substances, and dissolving the effective substances into a pre-prepared liquid with a certain concentration by using a certain amount of 1, 3-propanediol and PEG-40 hydrogenated castor oil.

The weight ratio of the pseudo-ginseng extract to the 1, 3-propanediol to the PEG-40 hydrogenated castor oil is as follows: 1-3:70-90:10-25, preferably 1.5:78.5:20.

Wherein the Notoginseng radix extract is Notoginseng radix (PANAX NOTOGINSENG) extract.

The lactobacillus/lactobacillus fermentation product pre-prepared liquid is prepared by fermenting black soybean milk and lactobacillus in a fermentation tank, extracting active ingredients after fermentation and inactivation and wall breaking, and adding natural 1, 3-propanediol into the extracted fermentation liquid as a preservative.

The weight ratio of the black soybean milk to the lactobacillus to the 1, 3-propanediol is as follows: 60-80:0.02-0.2:20-35, preferably 69.9:0.1:30.

Wherein the LACTOBACILLUS/SOYBEAN milk fermentation product is LACTOBACILLUS/SOYBEAN EXTRACT fermentation product filtrate (Lactobacillus/SOYBEAN EXTRACT FERMENT FILTRATE).

The rhizoma bletillae extract pre-prepared liquid is prepared by extracting fresh rhizoma bletillae by a modern process technology to prepare an extract with a certain concentration, and adding natural 1, 3-propanediol as a preservative.

The weight ratio of the bletilla striata extract to the 1, 3-propanediol is as follows: 80-100:10-20, preferably 90:10.

Wherein the rhizoma BLETILLA extract is rhizoma BLETILLA (Bletilla strata) extract.

The tetrahydropyrimidine carboxylic acid is tetrahydropyrimidine carboxylic acid (ECTOIN).

The cross-linked polymer comprises glycerin, butanediol, sodium hyaluronate, betaine, EDTA disodium, panthenol, dipotassium glycyrrhizinate, beta-glucan, chitosan and polyacrylate cross-linked polymer-6. Are available from commercial suppliers of compliant cosmetic raw materials.

Further, a moisturizing lotion with soothing effect is provided, which comprises 83.99kg of water, 3kg of glycerin, 2kg of butanediol, 0.18kg of sodium hyaluronate, 2kg of betaine, 0.03kg of beta-glucan, 1kg of chitosan, 3kg of pseudo-ginseng extract pre-preparation, 1kg of purslane extract pre-preparation, 1.2kg of lactobacillus/soybean milk fermentation product, 0.3kg of tetrahydropyrimidine carboxylic acid, 0.1kg of bletilla striata extract, 1.2kg of PHL, 60.5kg of polyacrylate crosslinked polymer, 0.05kg of EDTA disodium, 0.1kg of panthenol and 0.05kg of dipotassium glycyrrhizinate.

The preparation method of the moisturizing lotion with the relieving effect comprises the following steps of:

(1) Adding raw material water into an emulsifying pot, adding raw material glycerin, sodium hyaluronate, beta-glucan and polyacrylate crosslinked polymer-6, uniformly mixing, adding into the emulsifying pot, stirring and dissolving completely.

(2) Adding trehalose, chitosan, notoginseng radix extract pre-mixed solution, herba Portulacae extract pre-mixed solution, lactobacillus/soybean milk fermentation product, tetrahydropyrimidine carboxylic acid, rhizoma Bletillae extract and PHL into emulsifying pot, stirring and dissolving completely.

Wherein the condition of the step (1) is that the stirring time is 50-70 minutes, the temperature is room temperature, the vacuum degree is-0.01-0.03 MPa, and the preferable condition is that the stirring time is 60 minutes, the temperature is room temperature, and the vacuum degree is-0.03 MPa.

The condition of the step (2) is that the stirring time is 20-30 minutes, the temperature is room temperature, the vacuum degree is-0.01-0.03 MPa, and the preferable condition is that the stirring time is 30 minutes, the temperature is room temperature, and the vacuum degree is-0.03 MPa.

Specifically, the repairing cream with the effects of relieving and moisturizing comprises water, betaine, butanediol, beta-glucan, dipotassium glycyrrhizinate, disodium EDTA, pseudo-ginseng extract pre-preparation liquid, sodium hyaluronate, cetostearyl glucoside/sorbitan olive oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate, tocopherol, purslane extract pre-preparation liquid, bletilla extract, preservative, polyacrylate-13, polyisobutylene, polysorbate-20 and sorbitan isostearate.

A repairing cream with the effects of relieving and moisturizing contains, by weight, 48-71% of water, 1-3% of betaine, 2-5% of butanediol, 0.02-0.055% of beta-glucan, 0.05-0.1% of dipotassium glycyrrhizinate, 0.05-0.2% of EDTA disodium, 1-6% of pseudo-ginseng extract pre-prepared liquid, 0.1-0.3% of sodium hyaluronate, 2.5-5% of cetylglucoside/sorbitan olive oleate, 2-5% of caprylic/capric triglyceride, 1-3% of polydimethylsiloxane, 3-5% of shea butter, 1-2% of jojoba oil, 1-4% of squalane, 0.5-2% of prinsepia utilis royle oil, 0.5-2% of phytosterol oleate, 0.5-2% of olive oil ceramide, 2% of isononyl isononanoate, 0.2-0.5% of tocopherol, 1-3% of extract pre-prepared liquid, 0.2-2% of rhizoma bletillae extract, 1-3% of preservative, 1-4% of polysorbate, 1-3.03-0.02% of polysorbate, and 1-0.3% of polysorbate and 1-0.02-12% of isosorbide.

Wherein sodium hyaluronate, trehalose, beta-glucan, shea butter, jojoba oil and squalane are used as moisturizers.

Wherein Notoginseng radix extract, herba Portulacae extract, prinsepia utilis royle oil are active substances with relieving effect.

Wherein rhizoma bletilla extract and ceramide are repairing active substances.

Wherein the preservative is PHL and consists of octanoyl hydroxamic acid, 1, 2-hexanediol and 1, 3-propanediol.

The shea butter is butter (BUTYROSPERMUM PARKII) of Butyrospermum parkii.

The jojoba oil is jojoba (SIMMONDSIA CHINENSIS) seed oil.

The Prinsepia utilis royle oil is Prinsepia utilis (PRINSEPIA UTILIS) oil.

The ceramide is prepared by mixing ceramide NP (CERAMIDE NP) with olive (Olea EUROPAEA) fruit oil at a ratio of 1:6.5.

The betaine, butanediol, beta-glucan, dipotassium glycyrrhizinate, disodium EDTA, cetostearyl glucoside, sorbitan olive oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, isononyl isononanoate, tocopherol, polyacrylate-13, polyisobutylene, polysorbate-20, water, sorbitan isostearate are available from the commercial suppliers of the raw materials of the cosmetics.

Further, there is provided: a repair cream with soothing and moisturizing effects comprises 66.15kg of water, 2kg of betaine, 4kg of butanediol, 0.03kg of beta-glucan, 0.05kg of dipotassium glycyrrhizinate, 0.05kg of disodium EDTA, 3kg of radix Notoginseng extract pre-formulation, 0.12kg of sodium hyaluronate, 3.5kg of cetostearyl glucoside/sorbitan olive oleate, 3kg of caprylic/capric triglyceride, 1.5kg of polydimethylsiloxane, 3.5kg of shea butter, 1.2kg of jojoba oil, 2kg of squalane, 0.8kg of Prinsepia utilis oil, 0.5kg of phytosterol oleate, 0.7kg of olive oil ceramide, 3kg of isononyl isononanoate, 0.2kg of tocopherol (vitamin E), 2kg of herba Portulacae extract pre-formulation, 0.5kg of bletilla extract, 1.2kg of PHL, 0.2kg of polyacrylate, 0.2kg of polyisobutene, 1.2kg of polysorbate-200.1 kg of sorbitan isostearate, and 0.08kg of sorbitan isostearate.

The repairing cream with the soothing and moisturizing effects comprises the following steps of:

(1) Adding raw material water into a water phase pot, uniformly mixing raw materials including butanediol, beta-glucan and sodium hyaluronate, adding into the water phase pot, adding raw materials including pseudo-ginseng pre-prepared liquid, betaine, dipotassium glycyrrhizinate and disodium EDTA, stirring and heating.

(2) The raw materials of cetostearyl glucoside, sorbitan olive oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate and tocopherol (vitamin E) are added into an oil phase pot, and heated and dissolved completely.

(3) Respectively pumping the water phase pot and the oil phase pot into an emulsifying pot, homogenizing and emulsifying, adding the raw materials (polyacrylate-13, polyisobutylene, polysorbate-20, water and sorbitan isostearate) after emulsifying, and stirring and mixing uniformly.

(4) Stirring and cooling.

(5) Adding herba Portulacae extract pre-mixed solution, rhizoma Bletillae extract and PHL into emulsifying pot, stirring and mixing.

Wherein the condition of the step (1) is that the stirring time is 30-60 minutes, the temperature is 75-85 ℃, the vacuum degree is 0, and the preferable condition is that the stirring time is 50 minutes, the temperature is 78 ℃, and the vacuum degree is 0.

The condition of the step (2) is that the stirring time is 15-30 minutes, the temperature is 75-85 ℃, the vacuum degree is 0, and the preferable condition is that the stirring time is 20 minutes, the temperature is 82 ℃, and the vacuum degree is 0.

The condition of the step (3) is that the stirring time is 10-20 minutes, the temperature is 75-85 ℃, the vacuum degree is-0.01-0.03 MPa, and the preferable condition is that the stirring time is 15 minutes, the temperature is 78 ℃, and the vacuum degree is-0.03 MPa.

The condition of the step (4) is that the stirring time is 30-60 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.02-0.05 MPa, and the preferable condition is that the stirring time is 50 minutes, the temperature is 40 ℃, and the vacuum degree is-0.03 MPa.

The condition of the step (5) is that the stirring time is 10-20 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.03-0.07 MPa, and the preferable condition is that the stirring time is 15 minutes, the temperature is 38 ℃, and the vacuum degree is-0.05 MPa.

Finally, a skin care product set composition with a soothing effect is also provided, which comprises the facial cleanser, the moisturizing lotion and the repairing cream.

Drawings

FIG. 1 comparison of inhibition ratio of LPS-induced RAW264.7 cells to inflammatory factor secretion by test samples

Test sample 1 #: moisturizing lotion (3.2 mg/ml) prepared in example 2

Test sample No. 2: repair cream prepared in example 3 (0.8 mg/ml)

3# test sample: special soothing moisturizing cream (0.8 mg/ml) for certain international brand

Test sample No. 4: some domestic brand Shu Minbao wet special cream (0.8 mg/ml)

FIG. 2 T8 subject facial VISIA image contrast

Figure 312 28 day facial VISIA data comparison of 312 subjects

Graph 412 subjects 28 day cheek skin stratum corneum moisture content comparison

Figure 512 subjects 28 day cheek skin transdermal moisture loss TWEL comparison

FIG. 6 comparison of the activities of rhizoma Bletillae extracts at different concentrations on HaCaT keratinocyte CCK-8 cells

FIG. 7 effect of anatomic scopy on invasion of skin texture by bletilla gum particles of activated carbon

Skin moisture/oil content change over 150 minutes after test sample application for the subject of FIG. 8

FIG. 9 Effect of bletilla striata extract and repair cream on HaCaT cell migration Activity

FIG. 10 Effect of bletilla striata extract and repair cream on HaCaT cell migration Activity

* Represents p <0.05 by t-test compared with the blank sample group

# represents p <0.05 by t-test compared with the control sample group

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention. The features and capabilities of the present invention are described in further detail below in connection with the examples.

Example 1 preparation of a facial cleanser

The reagents and instrumentation used in the following examples were as follows:

reagent:

EDTA disodium, sodium cocoyl glycinate, glycerol, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, hydroxypropyl trimethyl ammonium chloride hyaluronic acid, PHL, herba Portulacae extract pre-formulation, PEG-7 glycerol cocoate was purchased from Yunnan Bai Nilan resource development Co.

Instrument:

the batching kettle is purchased from Gaoyou Instrument factory, model XY-A

Electronic platform balance, purchased from Xiamen Bairensi technology, model TCS-T01R-150

Electronic balance available from Mettler Toledo, model PL1002E/02

This example is used to illustrate the preparation method of the facial cleanser.

Comprises EDTA disodium 0.1kg, cocoyl glycine sodium 12kg, glycerin 18kg, polyquaternium-100.1 kg, sodium methyl cocoyl taurate 2.4kg, sodium lauroyl isethionate 3kg, citric acid 1.1kg, hydroxypropyl trimethyl ammonium chloride hyaluronic acid 0.1kg, PHL1.2kg, herba Portulacae extract pre-preparation solution 0.8kg, PEG-7 glycerin cocoate 1.5kg, and water 59.7kg.

The preparation method comprises the following steps:

(1) Adding raw material water, polyquaternium-10, sodium cocoyl glycinate, glycerol, sodium methyl cocoyl taurate and sodium lauroyl isethionate into an emulsifying pot, stirring and heating, adding raw material citric acid and EDTA disodium, stirring and dissolving completely. The stirring time is 50 minutes, the temperature is 80 ℃, and the vacuum degree is-0.02.

(2) And (3) cooling: stopping heating, and continuously stirring and cooling. Stirring time is 55 minutes, temperature is 38 ℃, and vacuum is-0.02 MPa.

(3) And (3) completely dissolving the raw material hydroxypropyl trimethyl ammonium chloride hyaluronic acid with a proper amount of water, adding the mixture into an emulsifying pot, and uniformly stirring and mixing the mixture. Stirring time is 10 minutes, temperature is 37 ℃, and vacuum is-0.02 MPa.

(4) Adding PHL and herba Portulacae extract pre-mixed solution and PEG-7 glycerol cocoate into emulsifying pot, stirring, and mixing. Stirring time is 15 minutes, temperature is 37 ℃, and vacuum is-0.02 MPa.

EXAMPLE 2 preparation of moisturizing lotion

reagent:

glycerol, butanediol, sodium hyaluronate, betaine, disodium EDTA, panthenol, dipotassium glycyrrhizinate, beta-glucan, acetylglucosamine, notoginseng radix extract pre-preparation, herba Portulacae extract pre-preparation, lactobacillus/soybean milk fermentation product, tetrahydromethylpyrimidic acid, rhizoma bletilla extract, PHL, and polyacrylate crosslinked polymer-6 purchased from Yunnan Bai Nilan resource development Co.

Instrument:

the batching kettle is purchased from Gaoyou Instrument factory, model XY-A

Electronic balance available from Mettler Toledo, model PL1002E/02

This example is used to illustrate the preparation method of the moisturizing lotion of the present invention.

Comprises glycerol 3kg, butanediol 2kg, sodium hyaluronate 0.18kg, betaine 2kg, EDTA disodium 0.05kg, panthenol 0.1kg, dipotassium glycyrrhizinate 0.05kg, beta-glucan 0.03kg, chitosan 1kg, notoginseng radix extract pre-preparation 3kg, herba Portulacae extract pre-preparation 1kg, lactobacillus/soybean milk fermentation product 1.2kg, tetrahydropyrimidine carboxylic acid 0.3kg, rhizoma Bletillae extract 0.1kg, PHL 1.2kg, polyacrylate crosslinked polymer-60.5 kg, and water 83.99kg.

The preparation method comprises the following steps:

(1) Adding raw material water into an emulsifying pot, adding raw material glycerin, sodium hyaluronate, beta-glucan and polyacrylate crosslinked polymer-6, uniformly mixing, adding into the emulsifying pot, stirring and dissolving completely. The stirring time is 60 minutes, the temperature is room temperature, and the vacuum degree is-0.03 Mpa.

(2) Adding trehalose, chitosan, notoginseng radix extract pre-mixed solution, herba Portulacae extract pre-mixed solution, lactobacillus/soybean milk fermentation product, tetrahydropyrimidine carboxylic acid, rhizoma Bletillae extract and PHL into emulsifying pot, stirring and dissolving completely. The stirring time is 30 minutes, the temperature is room temperature, and the vacuum degree is-0.03 MPa.

EXAMPLE 3 preparation of repair cream

reagent:

betaine, butylene glycol, beta-glucan, dipotassium glycyrrhizinate, disodium EDTA, pseudo-ginseng extract pre-formulation, sodium hyaluronate, cetostearyl glucoside/sorbitan olive oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate, tocopherol, purslane extract pre-formulation, bletilla extract, PHL, polyacrylate-13, polyisobutylene, polysorbate-20, water, sorbitan isostearate were purchased from yunnan Bai Nilan resource development limited.

Instrument:

vacuum homogenizing emulsifying machine, model XY-A, purchased from Gaoyou Xinmail instruments factory, jiangsu province

Electronic balance available from Mettler Toledo, model PL1002E/02

This example is a description of the preparation method of the repair cream of the present invention.

Comprises betaine 2kg, butanediol 4kg, beta-glucan 0.03kg, dipotassium glycyrrhizinate 0.05kg, disodium EDTA 0.05kg, radix Notoginseng extract pre-formulation 3kg, sodium hyaluronate 0.12kg, cetostearyl glucoside/sorbitan olive oleate 3.5kg, caprylic/capric triglyceride 3kg, polydimethylsiloxane 1.5kg, shea butter 3.5kg, jojoba oil 1.2kg, squalane 2kg, prinsepia utilis oil 0.8kg, phytosterol oleate 0.5kg, olive oil ceramide 0.7kg, isononyl isononanoate 3kg, tocopherol (vitamin E) 0.2kg, herba Portulacae extract pre-formulation 2kg, bletilla extract 0.5kg, PHL 1.2kg, polyacrylate-13, polyisobutene, poly-20, sorbitan isostearate 1kg, and water 66.15kg.

The preparation method comprises the following steps:

(1) Adding raw material water into a water phase pot, uniformly mixing raw materials including butanediol, beta-glucan and sodium hyaluronate, adding into the water phase pot, adding raw materials including pseudo-ginseng pre-prepared liquid, betaine, dipotassium glycyrrhizinate and disodium EDTA, stirring and heating. The stirring time was 50 minutes, the temperature was 78℃and the vacuum degree was 0.

(2) The raw materials of cetostearyl glucoside, sorbitan olive oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate and tocopherol (vitamin E) are added into an oil phase pot, and heated and dissolved completely. The stirring time was 20 minutes, the temperature was 82℃and the vacuum degree was 0.

(3) Respectively pumping the water phase pot and the oil phase pot into an emulsifying pot, homogenizing and emulsifying, adding the raw materials (polyacrylate-13, polyisobutylene, polysorbate-20, water and sorbitan isostearate) after emulsifying, and stirring and mixing uniformly. The stirring time is 15 minutes, the temperature is 78 ℃, and the vacuum degree is-0.03 MPa.

(4) Stirring and cooling. The stirring time is 50 minutes, the temperature is 40 ℃, and the vacuum degree is-0.03 MPa.

(5) Adding herba Portulacae extract pre-mixed solution, rhizoma Bletillae extract and PHL into emulsifying pot, stirring and mixing. The stirring time is 15 minutes, the temperature is 38 ℃, and the vacuum degree is-0.05 MPa.

EXAMPLE 4 preparation of skin care compositions

The cleansing cream, moisturizing lotion and repairing cream of examples 1-3 were combined into a kit.

Test example 1 determination of Risk substance Limit of facial cleanser, moisturizing lotion, and repair cream

The test entrusts Guangzhou city health detection service limited company to detect, and the test is carried out according to the requirements of the national food and drug administration (cosmetic safety technical Specification) (2015 edition).

Test sample:

the facial cleanser prepared by the method of example 1;

a moisturizing lotion prepared by the method of example 2;

the repair cream prepared by the method of example 3.

The testing method comprises the following steps: the harmful substance detection method and the limit value requirements are shown in Table 1, and the physicochemical detection results are shown in Table 2

Table 1 physicochemical inspection method and limit value for each index

Harmful substances	Detection method	Limit value (mg/kg)
			Mercury	Inductively coupled plasma mass spectrometry	<1
Arsenic (As)	Inductively coupled plasma mass spectrometry	<2
			Cadmium (Cd)	Inductively coupled plasma mass spectrometry	<5
Lead	Inductively coupled plasma mass spectrometry	<10
			Dioxaalkanes	Gas chromatography-mass spectrometry	<30

TABLE 2 physicochemical test results of test samples

Conclusion: through detection, each physicochemical detection result of the sample meets the requirements of cosmetic safety technical Specification (2015 edition).

Test example 2 sanitary article limitation measurement of face cream, moisturizing lotion and repair cream

Test sample:

the facial cleanser prepared by the method of example 1;

a moisturizing lotion prepared by the method of example 2;

the repair cream prepared by the method of example 3.

The testing method comprises the following steps: the limit value of the detection of the microorganisms is shown in Table 3, and the detection result of the microorganisms is shown in Table 4.

Table 3 microorganism tests Each index and requirement

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TABLE 4 results of microbiological tests on test samples

Conclusion: through detection, all microorganism detection results of the sample meet the requirements of cosmetic safety technical Specification (2015 edition).

Test example 3 facial cleanser, moisturizing lotion, and repair cream Single skin irritation and multiple skin irritation test

Test sample:

the facial cleanser prepared by the method of example 1;

a moisturizing lotion prepared by the method of example 2;

the repair cream prepared by the method of example 3.

The testing method comprises the following steps:

test purpose: determining and evaluating whether the cosmetic raw material and its product have a stimulating or corroding effect on the skin of a mammal at a local site and its extent.

Basic principle: the test substance is applied to the skin of the test animal one (or more) times, and the degree of local skin irritation of the animal is observed and scored at regular time intervals. Self-controls were used to evaluate the skin irritation of the test subjects. The period of observation of the acute skin irritation test should be sufficient to evaluate the reversibility or irreversibility of the effect.

Evaluation results: the average daily integral per animal was calculated as follows, and skin irritation intensity was determined in tables 5 and 6.

Average integration per animal per day = (Σerythema and integration in water/subject animal)/14

TABLE 5 skin response and integration correspondence table

Skin reaction	Integration of
		Erythema and eschar formation
No erythema	0
		Slight erythema (barely visible)	1
Obvious erythema	2
		Moderate-severe erythema	3
Severe erythema (mauve) to slight eschar formation	4

Edema formation
		No edema	0
Slight edema (barely visible)	1
		Mild oedema (skin doming clear outline)	2
Moderate edema (skin doming about 1 mm)	3
		Severe edema (skin doming exceeding 1mm, enlarged range)	4
Highest integral	8

TABLE 6 integral mean and stimulus intensity correspondence table

Integral mean value	Stimulus intensity
		0～＜0.5	No irritation
0.5～＜2.0	Light irritation
		2.0～＜6.0	Mid-irritation
6.0～8.0	Strong irritation

Test results

The preparation is applied for 1 time a day, and the preparation is continuously applied with the facial cleanser and observed for 14 days. During the test period, no abnormal results were seen on the animal drug administration side and on the control skin. See table 7.

Table 7 table of skin irritation and response scores of test samples of cleanser to rabbits

Conclusion: according to the requirements of cosmetic safety technical Specification (2015 edition), the face cleaning milk can be judged to have no irritation to skin.

The product is applied for 1 time a day, and the product is continuously applied with moisturizing lotion and observed for 14 days. During the test period, no abnormal results were seen on the animal drug administration side and on the control skin. See table 8.

Table 8 moisture lotion test sample multiple skin irritation and response scoring table for rabbits

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Conclusion: according to the requirements of cosmetic safety technical Specification (2015 edition), the moisturizing lotion can be judged to be free from irritation to skin.

The cream is applied for 1 time a day, and the cream is continuously applied and observed for 14 days. During the test period, no abnormal results were seen on the animal drug administration side and on the control skin. See table 9.

Table 9 repair cream test samples multiple skin irritation and response scoring table for rabbits

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Conclusion: according to the requirements of cosmetic safety technical Specification (2015 edition), the moisturizing cream can be judged to have no irritation to skin.

Test example 4 evaluation of the inhibition of LPS-induced RAW264.7 cells by pre-formulated RAW materials

The evaluation and research are carried out by entrusting the scientific and technological achievement hatching center of Kunming medical university, and the evaluation is carried out according to the method of testing the cosmetic soothing efficacy test-in vitro TNF-alpha inflammatory factor content determination lipopolysaccharide induced macrophage RAW264.7 (T/SHRH 033-2020) of the group standard.

Study purposes: and (3) evaluating the relieving effect of the test sample serving as a cosmetic RAW material by using an anti-inflammatory effect model of inducing macrophage inflammatory cells (RAW 264.7) to secrete the content of inflammatory factor TNF-alpha by using Lipopolysaccharide (LPS).

Principle of: LPS-induced RAW264.7 is a classical cell model for the study of inflammatory factors. LPS and macrophage surface antigen recognition receptor are combined, so that the macrophage can be induced to secrete various inflammatory factors such as TNF-alpha and the like. TNF-alpha can activate three signal paths of Caspase protease, JNK and transcription factor NF- κB, and can implement biological functions of cytotoxicity, antivirus, immunoregulation and cell apoptosis. The method evaluates the effect of the test sample on inhibiting TNF-alpha secretion by comparing the difference in the amount of TNF-alpha secreted by RAW264.7 cells after administration of the negative control and the test sample. The specific principle of the enzyme-linked immunosorbent assay (ELISA) is as follows: after the TNF-alpha is specifically combined with the TNF-alpha antibody coated on the ELISA plate, the anti-TNF-alpha antibody with a substrate mark is combined, the substrate is catalyzed to generate a colored product, and the TNF-alpha content and the colored product are measured at the wavelength of 450nm to calculate the TNF-alpha content.

The research method comprises the following steps:

1. and (3) cells: the mouse mononuclear macrophage leukemia cell line RAW264.7 is derived from the Kunming cell bank of the typical culture preservation committee of China academy of sciences, and the model is KCB 200603YJ.

2. Culture conditions: DMEM medium, fetal bovine serum, trypsin/EDTA solution (0.25% pancreatin solution mixed 1:1 with 0.02mol/L EDTA solution), phosphate Buffer (PBS), cell culture flasks.

3. Stimulus: bacterial lipopolysaccharide (LPS Escherichia coli source)

4. Positive control: dexamethasone (Soy Laibao D8040)

5. Materials, instruments: a 96-well plate; TNF-alpha ELISA detection kitELISA kit); CCK-8 cytotoxicity assay kit (Soy Bao CA 1210); an enzyme-labeled instrument; an ultra-clean workbench; dimethyl sulfoxide (DMSO).

6. Study sample: see Table 10

Table 10 evaluation of sample grouping information

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7. The operation steps are as follows: lipopolysaccharide-induced macrophage RAW264.7 test method (T/SHRH 033-2020) according to the body Standard "cosmetic soothing efficacy test-in vitro TNF-. Alpha.inflammatory factor content determination".

TNF-alpha content and inhibition calculation

(1) TNF-alpha content

Preparing a standard product of TNF-alpha into a standard solution, gradually diluting the standard solution into a series of solutions with known concentrations according to a proportion, measuring the OD value (OD 450) of the standard solution at 450nm by using the ELISA method, fitting a regression equation for preparing a standard curve, carrying the OD450 value of each test sample by using a human equation, and calculating the result of the content of the TNF-alpha in the test sample. 3 duplicate wells were tested in parallel for each group and mean and Standard Deviation (SD) were calculated. The T-test analysis is carried out on the TNF-alpha content of each sample group measured by the test and the negative control group by using SPSS statistical software, and the difference of P < 0.05 is statistically significant.

(2) TNF-alpha inhibition rate

Inhibition (%) = (1-T/C) ×100%, where T is the average TNF- α content of the sample group and C is the average TNF- α content of the negative control group.

Study results:

TABLE 11 comparison of CCK-8 cell viability of different doses of test samples against inflammatory cells (RAW 264.7)

Table 11 compares CCK-8 cell viability assays of inflammatory cells of the inflammatory cell macrophage line (RAW 264.7) with conventional and pre-formulated purslane extracts, pseudo-ginseng extract, and PHL preservatives. The results show that the cell viability of the pre-prepared purslane extract and the pseudo-ginseng extract which take the 1, 3-propanediol of biological origin as a solvent and pre-prepare the PHL preservative system can be maintained to be more than 90% within the concentration range of 0.4-3.2 mg/ml, and the cell viability does not obviously fluctuate with the increase of the dosage. In contrast, the cell viability of the conventional purslane extract and the pseudo-ginseng extract using the conventional propylene glycol as the solvent is less than 90% under the same test conditions, and gradually decreases with increasing dose. The conventional propylene glycol is proved to have a certain irritation to RAW264.7 cells by being taken as a solvent, so that the cell viability of the RAW264.7 cells is reduced. In contrast, after the biological source of 1, 3-propanediol is used as a solvent and a PHL preservative system is pre-prepared, the RAW264.7 cells are not stimulated and the cell viability is not affected.

Table 12 compares inhibition of secretion of inflammatory factor TNF- α by LPS-induced RAW264.7 cells

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"x" indicates that the difference in TNF- α content in the test group compared to the negative control group has a statistical significance (P < 0.01) by t-test;

"#" indicates that the difference in inhibition of TNF-alpha by pseudo-ginseng extract prepared using biologically derived 1, 3-propanediol as solvent compared to conventional propanediol as solvent was statistically significant (P < 0.01) by t-test.

Table 12 shows that under the conditions of the test examples, whether the conventional or pre-formulated purslane extract or the pseudo-ginseng extract, the extract has a remarkable inhibition effect on secretion of inflammatory factor TNF-alpha by RAW264.7 cells induced by LPS. Wherein, the inhibition rate of the pre-prepared pseudo-ginseng extract on TNF-alpha is obviously higher than that of the pre-prepared pseudo-ginseng extract.

Conclusion: under the condition of the test example, both the purslane extract and the pseudo-ginseng extract have obvious effect of inhibiting the release of inflammatory factor TNF-alpha. Because the biological source 1, 3-propanediol is adopted as the solvent, and the pre-prepared purslane extract and the pseudo-ginseng extract of the pre-prepared PHL preservative system have lower cytotoxicity, the burden on the skin can be reduced, and the preparation is suitable for SS people to be used as daily skin care products for a long time.

Test example 5 evaluation of inhibition of LPS-induced RAW264.7 cells by moisturizing lotion and repair cream

The evaluation study entrusts the university of Kunming medical science scientific and technological achievement hatching center to conduct the evaluation according to the section of "6.3PS induced RAW264.7 cell model anti-inflammatory evaluation" in the group standard "safety/efficacy evaluation Standard of sensitivity-class efficacy skin Care products (T/CNMIA 0013-2020").

Study purposes: the anti-inflammatory efficacy model of inducing macrophage inflammatory cells (RAW 264.7) to secrete inflammatory factors TNF-alpha, IL-1 beta and IL-6 content by using Lipopolysaccharide (LPS) is used for evaluating and testing the relieving efficacy of the cosmetics.

Principle of: LPS-induced RAW264.7 is a classical cell model for the study of inflammatory factors. LPS and macrophage surface antigen recognition receptor are combined, so that the macrophage can be induced to secrete various inflammatory factors such as TNF-alpha, IL-1 beta, IL-6 and the like.

TNF-alpha can activate three signal paths of Caspase protease, JNK and transcription factor NF- κB, and can implement biological functions of cytotoxicity, antivirus, immunoregulation and cell apoptosis.

IL-1β is an important mediator of inflammatory responses and can be involved in a variety of cellular activities including cell proliferation, differentiation, apoptosis, etc. when the body is in an inflammatory state or an immune response occurs. IL-1β can also cause increases in acute phase reactive proteins such as C-reactive proteins, etc. by activating endothelial cells, causing bone marrow neutrophil mobilization (leukocytosis), etc., thereby causing systemic inflammation.

IL-6 produces different patterns in different inflammations. In the early stages of infectious inflammation, different pathogens stimulate the production of IL-6 by monocytes and macrophages through Toll-like receptors (TLRs) of the relevant molecular pattern (PAMP). In non-infectious inflammatory conditions (e.g., burns or traumatic injury), IL-6 production by injured cells is stimulated by the TLR of the injury-associated molecular pattern (DAMP). This acute IL-6 expression plays a central role in host defense by stimulating various cell populations.

The method evaluates the effect of the test sample on inhibiting TNF-alpha, IL-1 beta and IL-6 secretion by comparing the difference of the contents of the secreted TNF-alpha, IL-1 beta and IL-6 of RAW264.7 cells after the administration of the negative control and the test sample. The content measurement adopts an enzyme-linked immunosorbent assay (ELISA) method, and the specific principle is as follows: after inflammatory factors (TNF-alpha, IL-1 beta or IL-6) are specifically combined with the inflammatory factor antibody coated on the ELISA plate, the anti-inflammatory factor antibody with a substrate label is combined, a colored product is generated after the substrate is catalyzed, and the content of the inflammatory factors and the colored product are measured at the wavelength of 450nm to determine the optical density (OD value) so as to calculate the content of the inflammatory factors.

The research method comprises the following steps:

3. Stimulus: bacterial lipopolysaccharide (LPS Escherichia coli source)

4. Positive control: dexamethasone (Soy Laibao D8040)

5. Materials, instruments: a 96-well plate; TNF-alpha ELISA detection kit, IL-1 beta ELISA detection kit and IL-6ELISA detection kitELISA kit); CCK-8 cytotoxicity assay kit (Soy Bao CA 1210); an enzyme-labeled instrument; an ultra-clean workbench; dimethyl sulfoxide (DMSO).

6. Test packets: see Table 13

Table 13 evaluation of sample grouping information

7. The operation steps are as follows: according to the standards of the group. The section "6.3PS induced RAW264.7 cell model anti-inflammatory evaluation" in the "safety/efficacy evaluation criteria of comfort-type efficacy skin care products (T/CNMIA 0013-2020).

Calculation of TNF-alpha, IL-1 beta and IL-6 content and inhibition Rate

(1) Content of

Preparing standard substances of TNF-alpha, IL-1 beta and IL-6 into standard solutions respectively, diluting the standard solutions step by step according to a proportion into a series of solutions with known concentrations, measuring the OD value (OD 450) of the standard solutions at 450nm by using the ELISA method, fitting a regression equation for preparing a standard curve, carrying the OD450 value of each test sample with the equation, and calculating the result of the content of the TNF-alpha, the IL-1 beta and the IL-6 in the test sample. 3 duplicate wells were tested in parallel for each group and mean and Standard Deviation (SD) were calculated. The T-test analysis is carried out on the TNF-alpha, IL-1 beta and IL-6 contents of each sample group measured by the test and the negative control group by using SPSS statistical software, and the difference of P < 0.05 is statistically significant.

(2) TNF-alpha, IL-1 beta and IL-6 inhibition rates

Inhibition (%) = (1-T/C) ×100%, where T is the average of TNF- α, IL-1β or IL-6 content for each sample group and C is the average of TNF- α, IL-1β or IL-6 content for the negative control group.

Study results:

the present study adopts a cell model of LPS induced RAW264.7 cells to secrete three inflammatory factors, and compares the anti-inflammatory activity of the moisturizing lotion prepared in example 2 and the repairing cream prepared in example 3 with that of known cosmetics at home and abroad which claim the soothing efficacy. Table 14 shows that, except that the 3# test sample had no inhibitory effect on IL-1β, the 1# to 4# test samples had inhibitory effect on LPS-induced secretion of TNF- α, IL-1β and IL-6 by RAW264.7 cells. FIG. 1 compares the inhibition rates of three inflammatory factors for each test, 3.2mg/ml test sample # 1 (moisturizing lotion prepared in example 2) with inhibition rates of 47.22%, 53.80% and 54.35% for TNF- α, IL-1β and IL-6, respectively, and the test product has a soothing anti-inflammatory effect. Test sample # 2 (cream prepared in example 3) at 0.8mg/ml had inhibition of TNF- α, IL-1β and IL-6 at 71.45%, 72.27% and 91.23%, respectively.

Table 14 comparison of the inhibition of LPS-induced RAW264.7 cells against inflammatory factor secretion by test samples

Conclusion: under the test strip, test sample No. 1 (moisturizing lotion prepared in example 2, 3.2 mg/ml) and test sample No. 2 (repairing cream prepared in example 3, 0.8 mg/ml) have inhibition effect on secretion of TNF-alpha, IL-1 beta and IL-6 by RAW264.7 cells induced by LPS, which indicates that the test product has anti-inflammatory effect and meets the requirement of efficacy evaluation of skin care products with efficacy of allergy-relieving.

See fig. 1.

Test example 6 evaluation of skin State of Subjects after 14 days after the Combined use of face cleansing, moisturizing lotion and moisturizing cream

Lactic acid stinging experiment

To verify the effectiveness of using the product of the invention, 5 asian adult test volunteers aged 25-55 years were selected during the period 2022, 4, and lactic acid stinging tests were performed by skin subjects before and after using the product of the invention. The nasal labial area of the subject was selected for the test, 50 μl of 10% lactic acid solution was dropped on a single layer of filter paper having a diameter of 0.8cm, and placed on the nasal labial area of the subject, and the subject was asked to feel tingling and to observe skin reactions at 2.5 and 5 minutes, respectively, with the following specific scoring indexes:

table 15 lactic acid stinging criteria

The evaluation results were as follows:

table 16 lactic acid stinging evaluation test results

Subject numbering	2.5 minutes	For 5 minutes	Lactic acid stinging score
				1	0	0	0
2	2	1	3
				3	2	2	4
4	2	1	3
				5	2	2	4

From the results of the user's self-evaluation, 80% of the subjects selected were sensitive skin.

VISIA experiment

In order to verify the effectiveness of using the product of the invention, 5 Asian adult test volunteers aged 25-55 years are selected during 4 months of 2022, the facial skin is cleaned by using the facial cleanser, then the moisturizing lotion of the invention is smeared on the face for nursing, and then the moisturizing cream of the invention is smeared on the face for moisturizing and nourishing, once a day in the morning and evening for 14 days. After use, the skin characteristics were scored using an intelligent skin analyzer for facial skin spots, wrinkles, texture, pores, uv spots, brown spots, red spots, and purple as compared to other subjects of the same age, same gender, and same skin type.

The results are shown in Table 17.

TABLE 17 VISIA facial assessment results

Project	Subject 1	Subject 2	Subject 3	Subject 4	Subject 5
						Speckle pattern	90％	75％	66％	34％	66％
Wrinkles	29％	50％	89％	70％	34％
						Texture and method for producing the same	98％	96％	98％	97％	97％
Pores (pore)	54％	20％	10％	89％	37％
						Ultraviolet ray color spots	97％	81％	69％	78％	90％
Brown stain	96％	37％	63％	49％	40％
						Red zone	75％	40％	72％	62％	21％
Purple matter	63％	98％	91％	99％	95％

Remarks: the percentile score describes the scoring of skin characteristics compared to other individuals of the same age, sex, and skin type of the subject (the higher the score the better).

The product of the invention can obviously improve the indexes of the skin spots, the textures, the ultraviolet spots, the brown spots, the red areas, the ultraviolet spots and the like from the change results of the indexes of the skin spots, the wrinkles, the textures, the pores, the ultraviolet spots, the brown spots, the red areas, the ultraviolet spots and the like of the user.

Test example 7 combination of human skin safety assessment with the inventive product

1. Purpose(s)

The safety of the product combination against human skin was evaluated by trial of the product combination in humans for 28 days during which time the subject self-assessed and observed by the dermatologist.

2. Test method

1. Test sample

The facial cleanser prepared by the method of example 1; the moisturizing lotion prepared in the method of example 2; the repair cream prepared by the method of example 3.

2. A subject

Adult subjects 12 (male 6, female 6) were selected to meet the following inclusion criteria:

healthy adult aged 18-35 years;

no serious systemic disease, no immunological disease, no skin disease, no allergic skin;

the subject site has not received skin treatment, cosmetology, and other treatments that may affect the test results;

near one month and during this test, the subject was not using hormonal or immunosuppressive drugs;

The test site was in other clinical trials for the last month and during the present test;

agree to not use skin care products other than the test product during the test;

female subject is not in gestation or lactation.

3. Application method

The subjects used the facial cleanser, moisturizing lotion and cream prepared according to the present invention for personal skin care, once a day, each morning and evening, for 28 consecutive days, as follows. During this time, the subject is no longer using other skin cleansing and care products. The first step uses the facial cleanser and clear water to clean the face, and the second step evenly smears a proper amount of moisturizing lotion on the face, so that the face can be gently massaged until the moisturizing lotion is absorbed; and thirdly, uniformly applying a proper amount of repair cream on the face, and massaging gently until the repair cream is absorbed.

4. Security assessment

The subjects fill out the usage log based on subjective experiences during 28 days of use of the product combination, and record skin adverse reactions during the period.

Meanwhile, the dermatologist is asked to observe and evaluate the skin of the subject on days 7, 14 and 28 respectively, and evaluate adverse reactions such as xerosis cutis, desquamation, redness, pimple and the like caused by release and the potential possibility thereof. At the return visit of the subject, any adverse events that the subject had occurred during use of the product combination are carefully interrogated, inspected, and recorded, including findings, time of occurrence, treatment and prognosis of the adverse event, and a determination is made as to the relationship of the adverse event to the product combination used.

The skin adverse reaction grading is judged according to the skin reaction grading standard of the human trial test fixed in cosmetic safety technical Specification 2015, as follows:

TABLE 18 skin reaction analysis and criteria for human trial

The severity of the adverse event is judged according to the following three-level standard:

light: the subject can tolerate, does not need special treatment, and does not influence the normal life of the subject;

and (3) moderately: the subjects are intolerable, and the normal life of the subjects is affected by stopping use or performing special treatment;

severe: preventing the normal life of the subject, and immediately stopping the use or making emergency treatment.

Judgment standard of relationship between adverse event and test sample:

the positive relation is: the time sequence of using the test sample and the reaction is reasonable, the reaction is stopped by stopping using the test sample, or is rapidly lightened or improved, the reaction is reproduced by using the test sample again, and meanwhile, the test sample has document data evidence and the influence of other confounding factors is eliminated;

it is likely that this is relevant: the time sequence of using the test sample and the reaction is reasonable, and the reaction of stopping using the test sample is stopped, or is rapidly lightened or improved, so that the influence of other factors can be basically discharged;

may be concerned: the use of test samples and the reaction time are closely related, but more than one sample causing adverse reaction can not be used, or other factors can not be excluded;

Possibly irrelevant: the test sample and adverse reaction are used, the time relationship is not close, and the reaction is found to be inconsistent with the known adverse reaction of cosmetics;

and (3) to be evaluated: incomplete data, evaluation after waiting for supplementary data, or difficult theoretic causal relationship, and lack of document data evidence;

failure to evaluate: the defects are too many, the causal relationship is difficult to determine, and the data cannot be supplemented.

3. Test results

Table 19 subjects were evaluated for adverse reactions within 28 days of using the product combination

4. Conclusion(s)

The combination of the product was used for skin cleansing and care for 28 consecutive days, and no discomfort and no skin adverse reaction were observed in 12 subjects.

Test example 8 evaluation of skin tolerance to lactic acid stimulation in combination with the use of the product of the invention

1. Purpose(s)

The 12 subjects were evaluated for differences in skin tolerance to lactic acid stimulation around 14 days after use of the product combination of the invention.

2. Test method

1. Test sample

2. A subject

Healthy adult aged 18-35 years;

female subject is not in gestation or lactation.

3. Application method

Subjects were divided into two groups of 6 persons each (male 3, female 3). One group was a treatment group, and subjects used personal skin care with the facial cleanser, moisturizing lotion, and cream prepared according to the present invention as follows, once a day, each morning and evening, for 14 days. During this time, the subject is no longer using other skin cleansing and care products. The first step uses the facial cleanser and clear water to clean the face, and the second step evenly smears a proper amount of moisturizing lotion on the face, so that the face can be gently massaged until the moisturizing lotion is absorbed; and thirdly, uniformly applying a proper amount of repair cream on the face, and massaging gently until the repair cream is absorbed.

4. Lactic acid stinging experiment

Subjects in the treatment group and the control group were subjected to lactic acid stinging test before and after 14 days, and the subjects were evaluated and compared by self-control to change the response to lactic acid stimulation. In the test, the subjects need to clean the face with clear water, apply 50 mu L of a single-layer filter paper with the diameter of 0.8cm, which is loaded with 10% lactic acid solution, to the nasolabial folds on one side of the subjects, inquire the subjects about the stinging sensation and the skin reaction at 3 and 5 minutes respectively, and record the subjective feeling scores of the skin of the subjects. 0 part of non-thorn pain, 1 part of light thorn pain, 2 parts of medium thorn pain and 3 parts of heavy thorn pain; the accumulated stinging sensation is more than or equal to 3 points and is the stinging sensation of lactic acid.

3. Test results

Table 20 comparison of lactic acid stinging score for subjects in the treated group versus the control group for 28 days

Of the 6 subjects in the treatment group, 5 (83.3% on) had a reduced score for the lactic acid stinging response. None of the 6 subjects in the control group had significantly reduced lactic acid stinging response, but instead had 3 scores increased.

Table 21 comparison of 28 day lactic acid stinging improvement in the treated group with the control group [ n, (%) ]

Because the number of test samples is limited and the diopter variation value does not meet the normal distribution, the rank sum test (Mann-Whitney U test) of the independent samples was performed on the lactic acid stinging score variation values of the two groups of subjects, and the result P <0.05, which indicates that the difference between the treatment group using the skin care product combination prepared according to the present invention and the control group using the own skin care product (without the pseudo-ginseng extract, purslane extract, bletilla extract active ingredient) for 14 days lactic acid stinging score variation was statistically significant.

4. Conclusion(s)

The combination of the product is used for cleaning and caring skin for 28 days continuously, and can effectively improve the response of the skin of a subject to lactic acid stinging.

Test example 9 facial skin evaluation of subjects in combination with the use of the product of the present invention

1. Purpose(s)

The improvement of the physiological state of facial skin in subjects using the product combination of the invention was assessed by measuring the subject's facial skin stratum corneum moisture content, skin transepidermal water loss TEWL values, and collecting facial skin VISIA images.

2. Test method

1. Test sample

2. A subject

Adult subjects (male 6, female 6) were selected to meet the following inclusion criteria:

healthy adult aged 18-35 years;

● No serious system diseases, no immunological diseases, no skin diseases and no allergic skin;

● Consent was given to use no skin care product other than the test product during the test;

● Female subjects are not in gestation or lactation period

● A lateral clip region skin stratum corneum moisture content <60c.u.;

skin moisture loss rate in a lateral nip region>12g/m ² ·h

3. Application method

4. Facial image analysis (VISIA)

On day 0 and day 28 of the combination of the product, after the subject had seen his face with clear water, i tried to rest in an environment with a temperature of 21±1 ℃ and a relative humidity of 50±10% for 30 minutes, please the dermatologist collect the photographed face images with a VISIA skin image analyzer, and analyze the comparative face texture, red differentiation.

5. Moisture content of facial skin horny layer

After the subject completes the facial VISIA image acquisition of the previous step, the left cheek of the subject was tested for stratum corneum moisture using a skin moisture tester (courage+khazaka, corneometer CM 825).

6. Percutaneous moisture loss of facial skin (TEWL)

After the subject has completed the previous facial skin cuticle moisture content test, the subject's right cheek is subjected to a cuticle moisture content test using a skin moisture loss test probe (courage+khazaka, tewameter TM 300).

7. Statistical analysis

The resulting data were analyzed by SPSS. The method comprises the steps of carrying out shape-Wilk normal distribution test and variance alignment test on various data collected before and after 28 days of using the product combination of various subjects, carrying out paired sample t-test after statistical significance, and carrying out test leveling a=0.05, wherein p <0.05 indicates that the difference is significant.

3. Test results

ViSIA skin imaging

Referring to FIG. 2 and FIG. 3

Table 22 facial VISIA data record for 28 days for 12 subjects

The subject was evaluated by the dermatologist to significantly improve both texture and red silk areas in facial skin VISIA imaging around 28 days in combination with the present product.

2. Moisture content of facial skin horny layer

Table 23 12 subjects 28 day cheek skin stratum corneum moisture content data record

See fig. 4.

Analysis of the data shows that the moisture content of the skin horny layer of the cheek of the subject is increased from 34.7+/-12.0% to 47.5+/-11.4% and the change rate is 37.0% when the product is used for comparison before and after 28 days. The difference was statistically significant (p < 0.05) by t-test of paired samples.

3. Percutaneous moisture loss of facial skin (TEWL)

Table 24 12 subjects 28 day cheek skin transdermal moisture loss TEWL data record

See fig. 5.

Analysis of the data shows that the TWEL of the skin of the cheek of the subject is increased from 19.4+/-5.0 to 16.1+/-5.6 and the change rate is-16.6% when the product is used for comparison before and after 28 days. The difference was statistically significant (p < 0.05) by t-test of paired samples.

4. Conclusion(s)

The combination of the product is continuously used for 28 days, improves various physiological indexes of the facial skin of a subject, can effectively relieve skin sensitivity, increase the water content of horny layer, improve the skin barrier function, and has certain effects of relieving, moisturizing and repairing the skin.

Test example 10 cytotoxicity evaluation of bletilla striata extract

1. Purpose(s)

The cytotoxicity of different concentrations of bletilla striata extracts on HaCaT keratinocytes was evaluated using the CCK-8 method.

2. Test method

1. And (3) cells: haCaT keratinocytes were derived from north na allied biotechnology limited.

Cck-8 assay: cells were seeded into 96-well plates at 200 μl cell suspension per well (8 ten thousand cells per well). Culturing in an incubator for 24 hours, adding substances to be detected with different concentrations into a culture plate, incubating for 24 hours, sucking supernatant, discarding, adding 100 mu l of culture medium and 10 mu l of CCK-8 solution mixture into each hole, incubating for 2 hours, and measuring absorbance at 450nm by using an enzyme-labeled instrument.

3. Test results

See fig. 6.

4. Conclusion(s)

According to CCK-8 results, it is believed that bletilla striata extracts showed no significant cytotoxicity to HaCaT keratinocytes, with cell viability >90% at a concentration of 5% or less.

Test example 11 evaluation of film Forming Property of bletilla extract

1. Purpose(s)

The bletilla striata extract was evaluated for film formation on the skin surface.

2. Test method

Selecting fresh and uniformly textured pigskin with shaved surface hair, cutting into pieces with size of 4X3 cm ² And are divided into three groups. Three groups of pigskin were treated with 120 μl of test samples (group a: 100% bletilla gum; group B: 20% bletilla gum; group C: deionized water), respectively. After 1.5 hours of standing at room temperature (22-26 ℃ C., relative humidity 40-60%) under a dissecting scope, observation and photographing are performed. Subsequently, the surface of the pigskin was treated with activated carbon powder (particle size about 75 μm), and photographed again under an anatomic lens before and after washing with water (without using any detergent)And (5) comparing.

3. Test results

See fig. 7.

4. Conclusion(s)

From the picture under the dissecting mirror, a protective film is formed after the bletilla gum is smeared and absorbed, so that the invasion of active carbon powder particles to skin textures can be effectively blocked, and the blocking effect of 100% of the bletilla gum is better than that of 20% of the bletilla gum.

Test example 12 evaluation of moisturizing efficacy of bletilla striata extract in moisturizing lotion

1. Purpose(s)

The moisturizing efficacy of bletilla striata extracts in moisturizing lotions and repair creams prepared according to the invention was evaluated.

2. Test method

1. Grouping

2. Operation of

3 healthy subjects were selected to clean the face using the facial cleanser prepared according to the present invention, and initial values of moisture and oil content of the skin horny layer of the subject's face were recorded using a skin moisture tester (Corneometer CM 825). The skin of the left side face of the subject is smeared with a proper amount of the moisturizing lotion A (or the moisturizing lotion B) prepared according to the invention, and the skin is gently massaged until the moisturizing lotion A or the moisturizing lotion B is absorbed; at the same time, the right-side skin was coated with the control sample in the same amount and coating method. The same procedure was used to test the moisture and oil content of the cheek skin on both sides of the subject at 0, 3, 5, 10, 30, 60, 90, 120 and 150 minutes using a skin moisture tester, and observe the change.

3. Test results

See fig. 8.

Experiments show that after the subject uses the moisturizing lotion A containing 2% of the bletilla extract and the moisturizing lotion B containing 1% of the bletilla extract, the moisture content of the skin cuticle is increased, the oil content is relatively reduced, the moisturizing lotion A can last for 150 minutes, and the moisturizing lotion B can last for about 90 minutes. In contrast, the skin of the subject without the control sample extracted from bletilla was allowed to recover the initial skin stratum corneum moisture/oil content at 45 minutes to the initial value.

4. Conclusion(s)

The rhizoma bletillae extract in the test sample can effectively prolong the duration of the moisturizing effect of the prepared moisturizing lotion on skin.

Test example 13 evaluation of repair efficacy of bletilla striata extract in repair cream

1. Purpose(s)

The effect of bletilla striata extracts and the repair cream prepared according to the invention on the cell migration of HaCaT was evaluated by scratch experiments.

2. Test method

1. And (3) cells: haCaT keratinocytes from north na allied biotechnology limited.

2. Grouping:

3. operation of

After resuspension of HaCaT cells grown in log phase, the cells were seeded in 6-well plates, the 6-well plates were placed at 37℃and 5% CO ₂ Culturing in an incubator until cells are fully spread in the wells. And (3) drawing a line from top to bottom in the same direction of each test hole by using the sterile gun head to vertically culture the holes, grasping the strength and the angle in the drawing process, enabling the line to be a straight line as much as possible, ensuring consistent width, finishing the drawing at one time, and avoiding repeated drawing. The medium was aspirated and purged 2 times with PBS buffer to wash away cell debris scraped off during streaking and cell clusters. After the PBS is absorbed and removed, four groups are arranged, each group is provided with three compound holes, fresh serum-free DMEM culture mediums of bletilla striata extracts, repair cream, control samples and blank samples are respectively added, so that cells are stimulated by medicines with gradient concentration, and the change of cell migration capacity is observed. Scratch changes were recorded with an inverted microscope for the same field of view at 0 hours and 24 hours. And calculating the scratch repair rate, and calculating the formula: scratch repair rate= (0 h scratch width-24 h scratch width)/0 h scratch width.

3. Test results

As shown in fig. 9 and 10, the 1% bletilla striata extract, the repair cream (containing 1% bletilla striata extract) and the control sample without the bletilla striata extract all significantly promoted the migration of HaCaT cells in the scratch test, and the scratch repair rate of the 1% bletilla striata extract and the repair cream (containing 1% bletilla striata extract) was significantly higher than that of the control sample without the bletilla striata extract (p <0.05 through t-test).

4. Conclusion(s)

The bletilla striata extract and the repairing cream (containing the bletilla striata extract 1%) with the content of 1% can effectively promote migration of HaCaT cells in scratch test, and has the effect of promoting repairing of skin injury.

In summary, the above-described kit, cleanser, moisturizing lotion or cream may be used for the care of sensitive skin, including cleaning, moisturizing, soothing and repairing.

Claims

1. The skin care product set composition with the relieving effect comprises a facial cleanser, a moisturizing lotion and a repairing cream, and is characterized in that the facial cleanser consists of water, EDTA disodium, a surfactant, a humectant, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, a preservative, a purslane extract pre-preparation liquid and PEG-7 glycerol cocoate; the moisturizing lotion comprises water, glycerol, butanediol, sodium hyaluronate, betaine, EDTA disodium, panthenol, dipotassium glycyrrhizinate, beta-glucan, chitosan, pseudo-ginseng extract pre-prepared liquid, purslane extract pre-prepared liquid, lactobacillus/soybean milk fermentation products, tetrahydropyrimidine carboxylic acid, bletilla striata extract, preservative and polyacrylate crosslinked polymer-6; the repairing cream consists of water, betaine, butanediol, beta-glucan, dipotassium glycyrrhizinate, EDTA disodium, pseudo-ginseng extract pre-prepared liquid, sodium hyaluronate, cetostearyl glucoside/sorbitan olive oleate, caprylic acid/capric acid triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate, tocopherol, purslane extract pre-prepared liquid, bletilla striata extract, a preservative, polyacrylate-13, polyisobutylene, polysorbate-20 and sorbitan isostearate; wherein the dosage of each component is as follows: the face cleaning emulsion comprises, by weight, 45-65% of water, 0.05-0.2% of EDTA disodium, 12-18% of a surfactant, 15-28% of a humectant, 100.1-0.3% of polyquaternium, 2-4% of sodium methyl cocoyl taurate, 3-5% of sodium lauroyl isethionate, 0.9-1.3% of citric acid, 1.0-1.4% of a preservative, 0.5-1.5% of a purslane extract pre-prepared solution and 1.0-2.5% of PEG-7 glycerol cocoate; the moisturizing lotion comprises, by weight, 80-90% of water, 3-5% of glycerol, 2-5% of butanediol, 0.12-0.24% of sodium hyaluronate, 1-3% of betaine, 0.05-0.2% of EDTA disodium, 0.1-0.5% of panthenol, 0.05-0.1% of dipotassium glycyrrhizinate, 0.02-0.05% of beta-glucan, 0.5-2% of chitosan, 2-5% of pseudo-ginseng extract pre-preparation, 0.5-2% of purslane extract pre-preparation, 0.5-2% of lactobacillus/soybean milk fermentation product, 0.1-0.5% of tetrahydropyrimidine carboxylic acid, 0.2-2% of bletilla striata extract, 1.0-1.4% of preservative and-60.3-0.7% of polyacrylate crosslinked polymer; the repairing cream comprises, by weight, 48-71% of water, 1-3% of betaine, 2-5% of butanediol, 0.02-0.055% of beta-glucan, 0.05-0.1% of dipotassium glycyrrhizinate, 0.05-0.2% of EDTA disodium, 1-6% of pseudo-ginseng extract pre-preparation, 0.1-0.3% of sodium hyaluronate, 2.5-5% of cetostearyl glucoside/sorbitan olive oleate, 2-5% of caprylic/capric triglyceride, 1-3% of polydimethylsiloxane, 3-5% of shea butter, 1-2% of jojoba oil, 1-4% of squalane, 0.5-2% of prinsepia utilis oil, 0.5-2% of phytosterol oleate, 0.5-2% of olive oil ceramide, 2-5% of isononyl isononanoate, 0.2-0.5% of tocopherol, 1-3% of purslane extract pre-preparation, 0.2-2% of bletilla extract, 1.0-1.4% of preservative, 3-130.0.0% of polyacrylate, 1-0.03% of polysorbate, and 200.02-0.0.02-0% of isosorbide; the purslane extract pre-prepared liquid is prepared by diluting purslane concentrated liquid for extracting effective matters with 1, 3-propanediol of biological origin and water to a certain concentration; the preservative is PHL and consists of octanoyl hydroxamic acid, 1, 2-hexanediol and 1, 3-propanediol.

2. The kit of claim 1 for use in the care of sensitive skin, said care comprising cleansing, moisturizing, soothing and repairing.

3. Use of the kit of parts according to claim 1 or 2 for the preparation of a product for the care of sensitive skin, said care comprising cleansing, moisturizing, soothing and repairing.