CN115337216A

CN115337216A - Skin care product composition with relieving effect and preparation thereof

Info

Publication number: CN115337216A
Application number: CN202210902770.XA
Authority: CN
Inventors: 陈真; 马任钟; 赵波; 赵琼; 夏金凤; 陈影; 陆地
Original assignee: Yunnan Qumei Biotechnology Co ltd; Kunming Medical University
Current assignee: Yunnan Qumei Biotechnology Co ltd; Kunming Medical University
Priority date: 2022-07-28
Filing date: 2022-07-28
Publication date: 2022-11-15
Anticipated expiration: 2042-07-28
Also published as: CN115337216B

Abstract

A product composition suitable for daily skin care of people with sensitive skin comprises a cleanser with cleaning effect, a moisturizing lotion with soothing effect and a repairing cream with soothing and moisturizing effects. The formula matrix of the oil-water emulsion is prepared from mild and non-irritant raw materials as far as possible, and essence, spice, preservative with high irritation, surfactant and cosolvent are avoided. For raw materials from natural products, the extract with controllable components and high purity is selected to be automatically prepared for use, finished raw materials containing irritant preservative and cosolvent are avoided, burden on skin is reduced as much as possible, and the natural skin care product is suitable for people with sensitive skin to serve as a daily skin care product and is used for a long time.

Description

Skin care product composition with relieving effect and preparation thereof

Technical Field

The invention relates to the field of cosmetics, and relates to a skin care product composition with a soothing effect for people with sensitive skin.

Background

Sensitive Skin (SS) is a highly reactive state of skin well developed on the face or has poor tolerance to stimulation, and can occur in physiological or pathological states, and the symptoms of the SS are mainly subjective symptoms such as burning, stabbing pain, pruritus and tightness of skin when the SS is stimulated by physical, chemical, mental and other factors, and can be accompanied by objective signs such as erythema, scale, telangiectasia and the like. The incidence rate among women is 15.93-23% in China and male 8.62%, and the actual incidence rate may be higher than that reported because the survey is limited by the area and number of people. Sensitive skin is highly prevalent worldwide, with 60-70% of women and 50-60% of men being reported abroad to have different degrees of skin sensitivity. The current research considers that the generation of SS is a complex process related to skin barrier-neurovascular-immune inflammation, but the 'gold standard' for SS treatment does not exist at home and abroad. In the 'Chinese sensitive skin diagnosis and treatment expert consensus' published in 2017 and the 'application guide of the skin care products for soothing and moisturizing in sensitive skin' published in 2019, the daily skin care products selected by the sensitive skin group follow the following principles: (1) the cosmetic ingredients should be mild and low in irritation; (2) On the basis of meeting the normal cleaning and moisturizing functions, the skin care cream has the effects of relieving (helping to improve the states of skin irritation and the like) and repairing (helping to maintain the skin to keep a normal state).

Daily improper skin care is the main causative factor of SS, including abusing cosmetics, over-cleansing, long-term exposure to irritating ingredients in cosmetics, and skin damage after medical and cosmetic treatment. In the ingredients of the usual cosmetic formulations, substances may be present which cause exogenous irritation to the skin, these irritants on the one hand possibly coming from preservatives (such as phenoxyethanol, potassium sorbate), surfactants (such as dodecylbenzene sulfonate), cosolvents (such as polyethylene glycol, propylene glycol), perfumes, etc.; on the other hand, when some natural product extracts of plant or animal origin having a mature supply system are used as cosmetic raw materials, it is also possible to introduce preservatives, co-solvents contained therein into the cosmetic, resulting in repeated addition of these potential irritants. Although the limited amount of the components is clearly specified in the technical Specification for cosmetic safety and has no risk to healthy skin, the components still have exogenous stimulation risk substances for the long-term use of SS people.

Disclosure of Invention

The invention provides a product combination suitable for daily skin care of SS people and a preparation method thereof, wherein the product combination comprises a cleanser with a cleaning effect, a moisturizing lotion with a relieving effect and a repairing cream with the relieving and moisturizing effects. The formula matrix of the oil-water emulsion is prepared from mild and non-irritant raw materials as far as possible, and essence, spice, preservative with high irritation, surfactant and cosolvent are avoided. For raw materials from natural products, the extract with controllable components and high purity is selected to be prepared and used automatically, finished raw materials containing irritant preservative and cosolvent are avoided, burden on skin is reduced as much as possible, and the natural skin care product is suitable for SS people to be used as daily skin care products for a long time.

The facial cleanser related by the invention takes a proper amount of sodium cocoyl glycinate as a surfactant; taking a proper amount of glycerin and hydroxypropyl trimethyl ammonium chloride hyaluronic acid as a humectant; a small amount of purslane extract is used as a soothing efficacy active substance; trace amount of caprylyl hydroximic acid is used as preservative. The facial cleanser has no irritation to skin and eyes, and skin is moistened and has no tightness after cleaning.

The moisturizing lotion takes a proper amount of sodium hyaluronate, trehalose, beta-glucan and acetyl chitosamine as moisturizing agents; a small amount of pseudo-ginseng extract, purslane extract and lactobacillus/soybean milk fermentation product are used as the soothing efficacy active substances; a small amount of tetrahydro-methyl pyrimidine carboxylic acid and bletilla extract are used as repairing active substances; trace amount of caprylyl hydroximic acid is used as preservative. The moisturizing lotion has no irritation to skin and eyes, and has remarkable allergy-relieving, anti-inflammatory and moisturizing effects.

The repair cream provided by the invention takes a proper amount of sodium hyaluronate, trehalose, beta-glucan, shea butter, jojoba oil and squalane as moisturizers; a small amount of pseudo-ginseng extract, purslane extract and prinsepia utilis royle oil are used as soothing active substances; a small amount of bletilla striata extract and ceramide are used as repairing active substances; trace amount of caprylyl hydroximic acid is used as preservative. The moisturizing cream has no irritation to skin and eyes, and has remarkable effects of relieving allergy, resisting inflammation, repairing skin barrier and moisturizing.

The facial cleanser, the moisturizing lotion and the repairing cream can be used independently or in combination. The composition can be used in combination with other active ingredients to produce better effect than single use, and can be used for people with sensitive skin to relieve skin problems.

In addition, the bletilla striata is derived from the dried tubers of the bletilla striata of the orchidaceae family, has the effects of astringing to stop bleeding, relieving swelling and promoting tissue regeneration as a traditional Chinese medicine, and has a long application history. Bletilla Striata Polysaccharide (BSP) is the main active component in the Bletilla Striata extract, wherein the BSP is Bletilla Striata mannan and consists of 4 molecules of mannose and 1 molecule of glucose. BSP is an excellent cosmetic raw material and has the characteristics of safety, moisture retention, film forming property and repairing activity: (1) Safety, the common bletilla gum is a traditional Chinese medicinal material which can be eaten and externally applied, and has a long use history. BSP is used as a natural polymer material, and a large number of research reports prove that BSP has no irritation and adverse reaction; (2) The moisture retention property is that BSP can absorb water molecules with the volume 200 times of the BSP to form viscous solution, and a layer of moisture retention film is formed on the surface of the skin to provide lasting moisture retention capacity for the skin; (3) The film forming property is that the BSP forms a film on the surface of the skin, so that the residence time of other active ingredients in the cosmetic on the surface of the skin can be increased, and the action time and effect of the active substances on the skin can be enhanced; (4) Repair activity, BSP activates multiple cytokines in the tissue repair process, playing a role in skin injury repair by inducing cell proliferation and cell migration. BSP activates macrophages, causing a cascade of responses that first cause activation of fibroblasts, which in turn produce epidermal growth factor (ECGF) and Angiogenic Factor (AF) on aging wrinkled skin. ECGF promotes the production of collagen and elastin, thereby improving skin appearance and eliminating wrinkles. The content of BSP in the bletilla striata extract used in the invention is more than 70%.

In order to achieve the purpose, the invention is realized by the following technical scheme:

the facial cleanser comprises water, disodium EDTA, a surfactant, a humectant, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, a preservative, a purslane extract pre-prepared solution and PEG-7 glycerol cocoate.

A cleansing milk with cleaning and moisturizing effects contains, by weight, 45-65% of water, 0.05-0.2% of EDTA disodium, 12-18% of a surfactant, 15-28% of a humectant, 100.1-0.3% of polyquaternium, 2-4% of sodium methyl cocoyl taurate, 3-5% of sodium lauroyl isethionate, 0.9-1.3% of citric acid, 1.0-1.4% of a preservative, 0.5-1.5% of a purslane extract pre-prepared liquid, and 1.0-2.5% of PEG-7 glyceryl cocoate.

Further, the surfactant is sodium cocoyl glycinate.

The humectant is glycerin and hydroxypropyl trimethyl ammonium chloride hyaluronic acid.

A cleansing cream with cleaning and moisturizing effects contains, by weight, 45-65% of water, 0.05-0.2% of EDTA disodium, 12-18% of sodium cocoyl glycinate, 15-25% of glycerol, 100.1-0.3% of polyquaternium-containing salt, 2-4% of sodium methyl cocoyl taurate, 3-5% of sodium lauroyl isethionate, 0.9-1.3% of citric acid, 0.05-0.15% of hydroxypropyl trimethylammonium chloride hyaluronic acid, 1.0-1.4% of preservative, 0.5-1.5% of purslane extract preparetion liquid and 1.0-2.5% of PEG-7 glyceryl cocoate.

Further, the purslane extract is an extract of purslane (portulaca OLERACEA).

The purslane extract pre-prepared liquid is prepared by diluting purslane concentrated liquid for extracting effective substances to a certain concentration by using 1, 3-propylene glycol and water which are biologically sourced. The 1, 3-propylene glycol is biological source, corn is biologically fermented, the purity of the 1, 3-propylene glycol can reach 99.8%, and the 1, 3-propylene glycol is a pure natural humectant with antiseptic effect and has no irritation or sensitization to skin.

The weight ratio of the purslane concentrated solution to the 1, 3-propylene glycol to the water is as follows: 0.2 to 0.8, preferably 0.4.

The antiseptic is PHL, and is composed of CAPRYLHYDROXAMIC ACID (caprilhydroxacic ACID), 1, 2-hexanediol, and 1, 3-propylene glycol.

Preferably, the weight ratio of CAPRYLHYDROXAMIC ACID (CAPRYLHYDROXAMIC ACID), 1, 2-hexanediol and 1, 3-propanediol is as follows: 1-8, preferably 5.

The PHL is prepared by adding caprylhydroxamic acid into 1, 3-propylene glycol and 1, 2-hexanediol, heating to 45-55 deg.C, stirring to dissolve completely, and cooling to room temperature.

The disodium EDTA, sodium cocoyl glycinate, glycerol, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, hydroxypropyl trimethyl ammonium chloride hyaluronic acid and PEG-7 glyceryl cocoate can be purchased from suppliers of raw materials of the conventional cosmetics.

Wherein, the 1, 3-propylene glycol is 100 percent of corn source, does not contain petroleum and animal raw materials, has high purity (up to 99.8 percent), has no irritation or sensitization to skin, and has the effects of antisepsis and moisture retention of cosmetics. 1, 3-propanediol is a better mild co-solvent alternative than 1, 2-propanediol, butanediol, which are petroleum sources.

The water is deionized water.

Further, there is provided: a facial cleanser with cleaning and moisturizing effects comprises 59.7kg of water, 12kg of sodium cocoyl glycinate, 18kg of glycerin, 100.1kg of polyquaternium, 0.1kg of disodium EDTA, 2.4kg of sodium methyl cocoyl taurate, 3kg of sodium lauroyl isethionate, 1.1kg of citric acid, 0.1kg of hydroxypropyl trimethyl ammonium chloride hyaluronic acid, 1.2kg of PHL2kg, 0.8kg of purslane extract prepartion liquid and 1.5kg of PEG-7 glyceryl cocoate.

The preparation method of the facial cleanser with the effects of cleaning and moisturizing comprises the following steps:

(1) Adding raw materials of water, polyquaternium-10, sodium cocoyl glycinate, glycerol, sodium methyl cocoyl taurate and sodium lauroyl isethionate into an emulsifying pot, stirring and heating, adding raw materials of citric acid and EDTA disodium, and stirring and dissolving completely.

(2) Cooling: stopping heating, and continuously stirring for cooling.

(3) Dissolving the raw material hydroxypropyl trimethyl ammonium chloride hyaluronic acid with a proper amount of water completely, adding the dissolved material into an emulsifying pot, and stirring and mixing uniformly.

(4) Adding PHL, herba Portulacae extract pre-prepared solution, and PEG-7 glyceryl cocoate into emulsifying pot, stirring, and mixing.

Wherein the conditions of the step (1) are that the stirring time is 40-60 minutes, the temperature is 75-85 ℃, the vacuum degree is-0.01 to-0.03 MPa, and the preferable conditions are that the stirring time is 50 minutes, the temperature is 80 ℃, and the vacuum degree is-0.02 MPa.

The conditions of the step (2) are that the stirring time is 40-60 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.01-0.03 MPa, and the preferable conditions are that the stirring time is 55 minutes, the temperature is 38 ℃, and the vacuum degree is-0.02 MPa.

The conditions of the step (3) are that the stirring time is 10-15 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.01 to-0.03 MPa, and the preferable conditions are that the stirring time is 10 minutes, the temperature is 37 ℃, and the vacuum degree is-0.02 MPa.

The conditions of the step (4) are that the stirring time is 10-15 minutes, the temperature is 35-45 ℃, the vacuum degree is-0.01 to-0.03 MPa, and the preferable conditions are that the stirring time is 15 minutes, the temperature is 37 ℃, and the vacuum degree is-0.02 MPa.

The moisturizing lotion comprises water, glycerol, butanediol, sodium hyaluronate, betaine, disodium EDTA, panthenol, dipotassium glycyrrhizinate, beta-glucan, acetyl chitosamine, pseudo-ginseng extract pre-prepared liquid, purslane extract pre-prepared liquid, lactobacillus/soybean milk fermentation products, tetrahydro-methylpyrimidine carboxylic acid, rhizoma bletillae extract, a preservative and polyacrylate cross-linked polymer-6.

A moisturizing lotion with a soothing effect comprises, by weight, 80-90% of water, 3-5% of glycerol, 2-5% of butanediol, 0.12-0.24% of sodium hyaluronate, 1-3% of betaine, 0.05-0.2% of disodium EDTA, 0.1-0.5% of panthenol, 0.05-0.1% of dipotassium glycyrrhizinate, 0.02-0.05% of beta-glucan, 0.5-2% of acetyl chitosamine, 2-5% of pseudo-ginseng extract pre-prepared liquid, 0.5-2% of purslane extract pre-prepared liquid, 0.5-2% of lactobacillus/soybean milk fermentation product, 0.1-0.5% of tetrahydro-methyl pyrimidine carboxylic acid, 0.2-2% of rhizoma bletillae extract, 1.0-1.4% of preservative, and 60.3-0.7% of polyacrylate cross-linked polymer.

Wherein sodium hyaluronate, betaine, beta-glucan and acetyl chitosamine are used as humectant.

Wherein the Notoginseng radix extract, herba Portulacae extract, and lactobacillus/soybean milk fermentation product are soothing active substances.

Wherein, the tetrahydro-methyl pyrimidine carboxylic acid and the bletilla striata extract are active matters with repairing efficacy.

Wherein the antiseptic is PHL.

The sodium hyaluronate is HA (molecular weight 10) ⁶ Da) and oligosaccharide HA (molecular weight 10) ⁴ Da) are mixed in a ratio of 3.

The notoginseng extract pre-prepared liquid is prepared by fermenting notoginseng extract by microorganism, separating and freeze-drying active substances, and dissolving the active substances into pre-prepared liquid with a certain concentration by using a certain amount of 1, 3-propylene glycol and PEG-40 hydrogenated castor oil.

The weight ratio of the pseudo-ginseng extract to the 1, 3-propylene glycol to the PEG-40 hydrogenated castor oil is as follows: 1-3.

Wherein the Notoginseng radix extract is Notoginseng radix (PANAX NONOGOINSENG) extract.

The lactobacillus/soybean milk fermentation product pre-prepared liquid is prepared by fermenting black soybean milk and lactobacillus in a fermentation tank, extracting active ingredients after fermentation inactivation and wall breaking, and adding natural 1, 3-propylene glycol as a preservative into the extracted fermentation liquid.

The weight ratio of the black soybean milk to the lactobacillus to the 1, 3-propylene glycol is as follows: 60-80, preferably 69.9.

Wherein the LACTOBACILLUS/soy milk fermentation product is LACTOBACILLUS/soy EXTRACT fermentation product FILTRATE (Lactobacillus/Soybean EXTRACT fermentation FILTRATE).

The bletilla striata extract pre-prepared liquid is prepared by extracting fresh bletilla striata through a modern process technology to prepare an extracting solution with a certain concentration, and adding natural 1, 3-propylene glycol as a preservative.

The weight ratio of the bletilla striata extract to the 1, 3-propylene glycol is as follows: 80-100, preferably 90.

Wherein the rhizoma Bletillae extract is rhizoma Bletillae (BLETILLA STRATA) extract.

The tetrahydromethyl pyrimidine carboxylic acid is tetrahydromethyl pyrimidine carboxylic acid (ECTOIN).

The glycerol, the butanediol, the sodium hyaluronate, the betaine, the EDTA disodium, the panthenol, the dipotassium glycyrrhizinate, the beta-glucan, the acetyl chitosamine and the polyacrylate cross-linked polymer-6. Are available from suppliers of compliant cosmetic raw materials.

Further, a moisturizing lotion with soothing effect is provided, which comprises 83.99kg of water, 3kg of glycerin, 2kg of butanediol, 0.18kg of sodium hyaluronate, 2kg of betaine, 0.03kg of beta-glucan, 1kg of acetyl chitosamine, 3kg of pseudo-ginseng extract pre-prepared liquid, 1kg of purslane extract pre-prepared liquid, 1.2kg of lactobacillus/soybean milk fermentation product, 0.3kg of tetrahydro-methylpyrimidine carboxylic acid, 0.1kg of bletilla striata extract, 1.2kg of PHL, 60.5kg of polyacrylate cross-linked polymer, 0.05kg of EDTA disodium, 0.1kg of panthenol and 0.05kg of dipotassium glycyrrhizinate.

The moisturizing lotion with the relieving effect is prepared by the following steps:

(1) Adding raw material water into an emulsifying pot, adding raw materials of glycerol, sodium hyaluronate, beta-glucan and polyacrylate cross-linked polymer-6, uniformly mixing, adding into the emulsifying pot, and stirring to dissolve completely.

(2) Adding trehalose, acetyl chitosamine, notoginseng radix extract pre-prepared solution, herba Portulacae extract pre-prepared solution, lactobacillus/soybean milk fermentation product, tetrahydro-methyl pyrimidine carboxylic acid, rhizoma Bletillae extract, and PHL into emulsifying pot, stirring, and dissolving completely.

Wherein the conditions of the step (1) are that the stirring time is 50-70 minutes, the temperature is room temperature, and the vacuum degree is-0.01 to-0.03 MPa, and the preferable conditions are that the stirring time is 60 minutes, the temperature is room temperature, and the vacuum degree is-0.03 MPa.

The conditions of the step (2) are that the stirring time is 20 to 30 minutes, the temperature is room temperature, and the vacuum degree is-0.01 to-0.03 MPa, and the preferable conditions are that the stirring time is 30 minutes, the temperature is room temperature, and the vacuum degree is-0.03 MPa.

Specifically, the repair cream with the effects of soothing and moisturizing comprises water, betaine, butanediol, beta-glucan, dipotassium glycyrrhizinate, disodium EDTA, pseudo-ginseng extract pre-formulation, sodium hyaluronate, cetearyl glucoside/sorbitan olivate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinus utilis oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate, tocopherol, purslane extract pre-formulation, bletilla striata extract, a preservative, polyacrylate-13, polyisobutylene, polysorbate-20 and sorbitan isostearate.

A repair cream with soothing and moisturizing effects comprises, by weight, 48-71% of water, 1-3% of betaine, 2-5% of butanediol, 0.02-0.055% of beta-glucan, 0.05-0.1% of dipotassium glycyrrhizinate, 0.05-0.2% of disodium EDTA, 1-6% of a notoginseng extract pre-prepared liquid, 0.1-0.3% of sodium hyaluronate, 2.5-5% of cetearyl glucoside/sorbitan olivate oleate, 2-5% of caprylic/capric triglyceride, 1-3% of polydimethylsiloxane, 3-5% of shea butter, 1-2% of jojoba oil, 1-4% of squalane, 0.5-2% of prinus utilis royle oil, 0.5-2% of phytosterol oleate, 0.5-2% of olive oil ceramide, 2-5% of isononyl isononanoate, 0.2-0.5% of tocopherol, 1-3% of a hyacinth bletilla extract pre-prepared liquid, 0.2-2% of a sorbitan ester, 1.4-1.4% of a preservative, 0.03-1.1.1.12% of polysorbate, 0.02-0.12% of polyisobutylene.

Wherein the sodium hyaluronate, trehalose, beta-glucan, shea butter, jojoba oil and squalane are used as humectant.

Wherein, the pseudo-ginseng extract, the purslane extract and the prinsepia utilis royle oil are active substances with the relieving effect.

Wherein, the bletilla striata extract and the ceramide are active substances with repairing efficacy.

Wherein the antiseptic is PHL, and is composed of caprylyl hydroximic acid, 1, 2-hexanediol, and 1, 3-propylene glycol.

The shea butter is Butyrospermum PARKII (Butyrospermum PARKII) butter.

The jojoba oil is jojoba (SIMMONDSIA CHINENSIS) seed oil.

The Prinsepia UTILIS Royle oil is Prinsepia UTILIS Royle (PRISEPIA UTILIS) oil.

The CERAMIDE is CERAMIDE NP (CERAMIDE NP) and olive (Olea EUROPAEA) fruit oil mixed according to a ratio of 1.

The betaine, butanediol, beta-glucan, dipotassium glycyrrhizinate, disodium EDTA, cetearyl glucoside, sorbitan olivate oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis oil, phytosterol oleate, isononyl isononanoate, tocopherol, polyacrylate-13, polyisobutylene, polysorbate-20, water, sorbitan isostearate can be purchased from a supplier of conventional cosmetic raw materials.

Further, there is provided: a repairing cream with soothing and moisturizing effects comprises 66.15kg of water, 2kg of betaine, 4kg of butanediol, 0.03kg of beta-glucan, 0.05kg of dipotassium glycyrrhizinate, 0.05kg of disodium EDTA, 3kg of a notoginseng extract pre-formulation solution, 0.12kg of sodium hyaluronate, 3.5kg of cetearyl glucoside/sorbitan olive oleate, 3.5kg of caprylic/capric triglyceride, 1.5kg of polydimethylsiloxane, 3.5kg of shea butter, 1.2kg of jojoba oil, 2kg of squalane, 0.8kg of prinsepia utilis royle oil, 0.5kg of phytosterol oleate, 0.7kg of olive oil ceramide, 3kg of isononyl isononanoate, 0.2kg of tocopherol (vitamin E), 2kg of a portulaca extract pre-formulation solution, 0.5kg of bletilla rhizome extract, 0.2kg of L, 131kg of polyacrylate, 0.2kg of polyisobutylene, 200.1kg of polysorbate, and 0.08kg of sorbitan isostearate.

The preparation method of the repair cream with the effects of relieving and moisturizing comprises the following steps:

(1) Adding raw material water into a water phase pot, uniformly mixing raw materials of butanediol, beta-glucan and sodium hyaluronate, adding into the water phase pot, adding raw materials of pseudo-ginseng pre-prepared liquid, betaine, dipotassium glycyrrhizinate and EDTA disodium, stirring and heating.

(2) Adding raw materials of cetearyl glucoside, sorbitan olive oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate and tocopherol (vitamin E) into an oil phase pot, and heating to completely dissolve.

(3) Pumping the raw materials into emulsifying pot, homogenizing, emulsifying, adding raw materials (polyacrylate-13, polyisobutylene, polysorbate-20, water, and sorbitan isostearate), stirring, and mixing.

(4) Stirring and cooling.

(5) Adding the purslane extract pre-prepared solution, the bletilla striata extract and the PHL into an emulsifying pot, and stirring and mixing uniformly.

Wherein the conditions of the step (1) are that the stirring time is 30-60 minutes, the temperature is 75-85 ℃, and the vacuum degree is 0, and the preferable conditions are that the stirring time is 50 minutes, the temperature is 78 ℃, and the vacuum degree is 0.

The conditions of the step (2) are that the stirring time is 15-30 minutes, the temperature is 75-85 ℃, and the vacuum degree is 0, and the preferable conditions are that the stirring time is 20 minutes, the temperature is 82 ℃, and the vacuum degree is 0.

The conditions of the step (3) are that the stirring time is 10 to 20 minutes, the temperature is 75 to 85 ℃, the vacuum degree is minus 0.01 to minus 0.03MPa, and the preferable conditions are that the stirring time is 15 minutes, the temperature is 78 ℃, and the vacuum degree is minus 0.03MPa.

The conditions of the step (4) are that the stirring time is 30 to 60 minutes, the temperature is 35 to 45 ℃, the vacuum degree is minus 0.02 to minus 0.05MPa, and the preferable conditions are that the stirring time is 50 minutes, the temperature is 40 ℃, and the vacuum degree is minus 0.03MPa.

The conditions of the step (5) are that the stirring time is 10 to 20 minutes, the temperature is 35 to 45 ℃, the vacuum degree is minus 0.03 to minus 0.07MPa, and the preferable conditions are that the stirring time is 15 minutes, the temperature is 38 ℃, and the vacuum degree is minus 0.05MPa.

Finally, a skin care product set composition with the soothing effect is also provided, and comprises the facial cleanser, the moisturizing lotion and the repair cream.

Drawings

FIG. 1 comparison of inhibition ratio of test samples on LPS-induced secretion of inflammatory factors from RAW264.7 cells

Test sample # 1: moisturizing lotion prepared in example 2 (3.2 mg/ml)

Test sample # 2: repair cream prepared in example 3 (0.8 mg/ml)

Test sample No. 3: special care moisturizing cream (0.8 mg/ml) for relieving certain international brand

Test sample # 4: certain domestic brand soothing and moisturizing special cream (0.8 mg/ml)

FIG. 2 T8 subject facial VISIA image contrast

FIG. 3 comparison of 28 day and face VISIA data for 12 Subjects

FIG. 4 comparison of skin stratum corneum Water content of cheek of 28 days in 12 subjects

FIG. 5 TWEL comparison of 28 day cheek skin transdermal Water loss in 12 subjects

FIG. 6 comparison of the activity of bletilla striata extracts at different concentrations on the CCK-8 cells of HaCaT keratinocytes

FIG. 7 shows the effect of bletilla striata gum on blocking activated carbon particles from attacking skin texture under a dissecting mirror

FIG. 8 change in skin Water/oil content 150 minutes after test sample application

FIG. 9 Effect of bletilla striata extracts and of treatment creams on HaCaT cell migration Activity

FIG. 10 Effect of bletilla striata extracts and of treatment creams on HaCaT cell migration Activity

* Indicating a p <0.05 by t-test as compared to the blank sample group

# denotes p <0.05 by t-test as compared to control sample group

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially. The features and properties of the present invention are described in further detail below with reference to examples.

EXAMPLE 1 preparation of facial cleanser

The reagents and instrumentation used in the following examples are as follows:

reagent:

disodium EDTA, sodium cocoyl glycinate, glycerin, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, hydroxypropyl trimethyl ammonium chloride hyaluronic acid, PHL, a purslane extract preformulation, and PEG-7 glyceryl cocoate were purchased from Yunnan Berniland resource development Co., ltd.

The instrument comprises:

the compounding kettle is purchased from Instrument plant of Gaoyou city, jiangsu province, and has model XY-A

Electronic platform scale purchased from mansion's Bai Lun science, model TCS-T01R-150

Electronic balance, model PL1002E/02 from Mettler Toledo

This example illustrates the preparation of the facial cleanser of the present invention.

Contains 0.1kg of EDTA disodium, 12kg of cocoyl glycine sodium, 18kg of glycerin, 100.1kg of polyquaternium, 2.4kg of methyl cocoyl sodium taurate, 3kg of sodium lauroyl isethionate, 1.1kg of citric acid, 0.1kg of hydroxypropyl trimethyl ammonium chloride hyaluronic acid, 1.2kg of PHLl, 0.8kg of purslane extract preparetion solution, 1.5kg of PEG-7 glyceryl cocoate and 59.7kg of water.

The preparation method comprises the following steps:

(1) Adding raw materials of water, polyquaternium-10, sodium cocoyl glycinate, glycerol, sodium methyl cocoyl taurate and sodium lauroyl isethionate into an emulsifying pot, stirring and heating, adding raw materials of citric acid and EDTA disodium, and stirring and dissolving completely. The stirring time was 50 minutes, the temperature was 80 ℃ and the vacuum degree was-0.02.

(2) Cooling: stopping heating, and continuously stirring for cooling. Stirring for 55 minutes at 38 ℃ under vacuum of-0.02 MPa.

(3) Dissolving the raw material hydroxypropyl trimethyl ammonium chloride hyaluronic acid with a proper amount of water completely, adding the dissolved material into an emulsifying pot, and stirring and mixing uniformly. Stirring for 10 minutes at 37 ℃ and vacuum-0.02 MPa.

(4) Adding PHL, herba Portulacae extract pre-prepared solution, and PEG-7 glyceryl cocoate into emulsifying pot, stirring, and mixing. The stirring time is 15 minutes, the temperature is 37 ℃, and the vacuum is-0.02 MPa.

Example 2 preparation of moisturizing lotions

The reagents and instrumentation used in the following examples are as follows:

reagent:

glycerin, butanediol, sodium hyaluronate, betaine, disodium EDTA, panthenol, dipotassium glycyrrhizinate, beta-glucan, acetyl chitosamine, pseudo-ginseng extract prepad liquid, purslane extract prepad liquid, lactobacillus/soybean milk fermentation product, tetrahydro-methyl pyrimidine carboxylic acid, bletilla striata extract, PHL% and polyacrylate cross-linked polymer-6 were purchased from Yunnan Bainielan resource development Co.

The instrument comprises:

Electronic platform scale, available from building door Bailun technology, model TCS-T01R-150

Electronic balance, from Mettler Toledo, model PL1002E/02

This example illustrates the preparation of a moisturizing lotion of the present invention.

Contains 3kg of glycerin, 2kg of butanediol, 0.18kg of sodium hyaluronate, 2kg of betaine, 0.05kg of EDTA disodium, 0.1kg of panthenol, 0.05kg of dipotassium glycyrrhizinate, 0.03kg of beta-glucan, 1kg of acetyl chitosamine, 3kg of pseudo-ginseng extract pre-prepared liquid, 1kg of purslane extract pre-prepared liquid, 1.2kg of lactobacillus/soybean milk fermentation product, 0.3kg of tetrahydro-methylpyrimidine carboxylic acid, 0.1kg of bletilla striata extract, 1.2kg of PHL, 60.5kg of polyacrylate cross-linked polymer and 83.99kg of water.

The preparation method comprises the following steps:

(1) Adding raw material water into an emulsifying pot, adding raw materials of glycerol, sodium hyaluronate, beta-glucan and polyacrylate cross-linked polymer-6, uniformly mixing, adding into the emulsifying pot, and stirring to dissolve completely. The stirring time is 60 minutes, the temperature is room temperature, and the vacuum degree is-0.03 MPa.

(2) Adding trehalose, acetyl chitosamine, notoginseng radix extract pre-prepared solution, herba Portulacae extract pre-prepared solution, lactobacillus/soybean milk fermentation product, tetrahydro-methyl pyrimidine carboxylic acid, rhizoma Bletillae extract, and PHL into emulsifying pot, stirring, and dissolving completely. The stirring time is 30 minutes, the temperature is room temperature, and the vacuum degree is-0.03 MPa.

Example 3 preparation of a repair cream

The reagents and instrumentation used in the following examples are as follows:

reagent:

betaine, butylene glycol, beta-glucan, dipotassium glycyrrhizinate, disodium EDTA, notoginseng extract pre-formulation, sodium hyaluronate, cetearyl glucoside/sorbitan olivate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate, tocopherol, purslane extract pre-formulation, bletilla striata extract, PHL, polyacrylate-13, polyisobutylene, polysorbate-20, water, sorbitan isostearate were purchased from conilan yunnanensis resource development limited.

The instrument comprises:

vacuum homogenizing and emulsifying machine, available from Xinmaili Instrument plant of Gaoyou city, jiangsu province, model XY-A

Electronic balance, model PL1002E/02 from Mettler Toledo

This example illustrates the preparation of a repair cream according to the invention.

Contains 2kg of betaine, 4kg of butanediol, 0.03kg of beta-glucan, 0.05kg of dipotassium glycyrrhizinate, 0.05kg of disodium EDTA, 3kg of notoginseng extract prepad liquid, 0.12kg of sodium hyaluronate, 3.5kg of cetearyl glucoside/sorbitan olivate oleate, 3kg of caprylic/capric triglyceride, 1.5kg of polydimethylsiloxane, 3.5kg of shea butter, 1.2kg of jojoba oil, 2kg of squalane, 0.8kg of prinus davidiana oil, 0.5kg of phytosterol oleate, 0.7kg of olive oil ceramide, 3kg of isononyl isononanoate, 0.2kg of tocopherol (vitamin E), 2kg of purslane extract prepad liquid, 0.5kg of bletilla rhizome extract, 1.2kg of PHL, polyacrylate-13, polyisobutylene, polysorbate-20, 1kg of sorbitan isostearate, and 66.15kg of water.

The preparation method comprises the following steps:

(1) Adding raw material water into a water phase pot, uniformly mixing raw materials of butanediol, beta-glucan and sodium hyaluronate, adding into the water phase pot, adding raw materials of pseudo-ginseng pre-prepared liquid, betaine, dipotassium glycyrrhizinate and EDTA disodium, stirring and heating. The stirring time was 50 minutes, the temperature was 78 ℃ and the vacuum was 0.

(2) Adding raw materials of cetearyl glucoside, sorbitan olive oleate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinsepia utilis royle oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate and tocopherol (vitamin E) into an oil phase pot, and heating to completely dissolve. The stirring time was 20 minutes, the temperature 82 ℃ and the vacuum 0.

(3) Pumping the raw materials into emulsifying pot, homogenizing, emulsifying, adding raw materials (polyacrylate-13, polyisobutylene, polysorbate-20, water, and sorbitan isostearate), stirring, and mixing. The stirring time is 15 minutes, the temperature is 78 ℃, and the vacuum degree is-0.03 MPa.

(4) Stirring and cooling. The stirring time is 50 minutes, the temperature is 40 ℃, and the vacuum degree is-0.03 MPa.

(5) Adding herba Portulacae extract pre-prepared solution, rhizoma Bletillae extract, and PHL into emulsifying pot, stirring, and mixing. The stirring time is 15 minutes, the temperature is 38 ℃, and the vacuum degree is-0.05 MPa.

EXAMPLE 4 preparation of skin Care compositions

The facial cleansers, moisturizers and repair creams of examples 1-3 were combined into a kit.

Test example 1 determination of Risk substance limits of face toilet, moisturizing lotion, and cream

The test was commissioned by Guangzhou city health testing services GmbH, and was performed according to the requirements of the State food and drug administration of general administration of cosmetics safety technical Specification (2015 Release).

Sample preparation:

the face cleanser prepared by the method of example 1;

a moisturizer prepared by the method of example 2;

the cream prepared by the method of example 3 was used.

The test method comprises the following steps: the detection method and limit requirements of harmful substances are shown in table 1, and the physicochemical detection results are shown in table 2

TABLE 1 physicochemical examination method for each index and limit

Hazardous substances	Detection method	Limit value (mg/kg)
			Mercury	Inductively coupled plasma mass spectrometry	<1
Arsenic (As)	Inductively coupled plasma mass spectrometry	<2
			Cadmium (Cd)	Inductively coupled plasma mass spectrometry	<5
Lead (II)	Inductively coupled plasma mass spectrometry	<10
			Dioxane(s)	Gas chromatography-mass spectrometry	<30

TABLE 2 results of physical and chemical examination of test samples

And (4) conclusion: through detection, the physical and chemical detection results of various samples meet the requirements of technical standards for cosmetic safety (2015 edition).

Test example 2 sanitary article limit measurement of face toilet, moisturizing lotion and repairing cream

Sample preparation:

the face cleanser prepared by the method of example 1;

a moisturizer prepared by the method of example 2;

a repair cream prepared using the method of example 3.

The test method comprises the following steps: the detection limits of microorganisms are shown in Table 3, and the detection results of microorganisms are shown in Table 4.

TABLE 3 microbiological test Each index and requirement

TABLE 4 test sample microbiological test results

And (4) conclusion: through detection, the detection results of various microorganisms in the sample all meet the requirements of technical standards for cosmetic safety (2015 edition).

Test example 3 Single skin irritation and multiple skin irritation tests of face cleanser, moisturizing lotion, and skin cream

Sample preparation:

the facial cleanser prepared by the method of example 1;

a moisturizer prepared by the method of example 2;

the cream prepared by the method of example 3 was used.

The test method comprises the following steps:

the purpose of the test is as follows: determining and evaluating whether the cosmetic raw materials and products thereof have irritation or corrosion effect on local skin of mammal and their degree.

Basic principle: the test substance is applied to the skin of a test animal once (or a plurality of times), and the degree of local irritation of the animal skin is observed and scored at prescribed time intervals. Self-control was used to evaluate the skin irritation of the test subjects. The acute skin irritation test observation period should be sufficient to evaluate the reversibility or irreversibility of the effect.

Evaluation results were as follows: the average integral per animal per day was calculated according to the following formula, and the skin irritation intensity was judged in tables 5 and 6.

Average integral per animal per day = (∑ erythema and underwater integral/test animal)/14

TABLE 5 skin reaction and integral correspondence table

Skin reactions	Integration
		Erythema and eschar formation
No erythema	0
		Mild erythema (barely visible)	1
Obvious erythema	2
		Moderate-severe erythema	3
Severe erythema (purplish red) to slight eschar formation	4

Edema formation
		Without edema	0
Slight edema (barely visible)	1
		Mild edema (clear outline of skin bulge)	2
Moderate edema (skin doming about 1 mm)	3
		Severe edema (skin doming over 1mm, extended range)	4
Highest integral	8

TABLE 6 correspondence table of integral mean value and stimulus intensity

Integral mean value	Intensity of stimulus
		0～＜0.5	Has no irritation
0.5～＜2.0	Light irritation
		2.0～＜6.0	Middle irritation
6.0～8.0	Strong irritation property

Test results

Applying the facial cleanser 1 time every day, continuously applying the facial cleanser, and observing for 14 days. During the test period, no abnormal results were observed on the skin of the animals at the administration side and the control side. See table 7.

Table 7 evaluation table of repeated skin irritation and reaction of face wash test samples to rabbits

And (4) conclusion: the cleanser can be judged to be non-irritating to the skin according to the requirements of technical safety regulations of cosmetics (2015 edition).

Applying the lotion for 1 time every day, continuously applying the lotion, and observing for 14 days. During the test period, no abnormal results were observed on the skin of the animals at the administration side and the control side. See table 8.

TABLE 8 moisture lotion test samples skin irritation and reaction evaluation table for rabbits for multiple times

And (4) conclusion: the moisturizing lotion can be judged to have no stimulation to the skin according to the requirements of technical Specification for safety of cosmetics (2015 edition).

Applying the ointment 1 time every day, continuously applying moisturizing cream and observing for 14 days. During the test period, no abnormal results were observed on the skin of the animals on the administration side and the control side. See table 9.

Table 9 evaluation table of multiple skin irritation and reaction of repair cream test sample to rabbit

And (4) conclusion: the moisturizing cream can be judged to be non-irritant to the skin according to the requirements of technical safety specifications of cosmetics (2015 edition).

Test example 4 evaluation of Effect of the Preset RAW Material on the inhibition of TNF-. Alpha.secretion from RAW264.7 cells induced by LPS

The evaluation study was conducted by the scientific and technological achievements incubation center of Kunming medical university, and the evaluation was conducted according to the group 'cosmetic soothing efficacy test-in vitro TNF-alpha inflammatory factor content determination lipopolysaccharide-induced macrophages RAW264.7 test method' (T/SHRH 033-2020).

The research aims are as follows: the soothing effect of the test sample as a cosmetic RAW material is evaluated by applying an anti-inflammatory effect model of Lipopolysaccharide (LPS) to induce macrophage inflammatory cell (RAW 264.7) to secrete the content of inflammatory factor TNF-alpha.

The principle is as follows: RAW264.7 is induced by LPS, a classical cell model for the study of inflammatory factors. LPS is combined with macrophage surface antigen recognition receptor, and can induce macrophage to secrete TNF-alpha and other various inflammatory factors. TNF-alpha can activate three signal paths of Caspase, JNK and transcription factor NF-kB, and realize the biological functions of cytotoxicity, virus resistance, immunoregulation, apoptosis and the like. The method evaluates the effect of the test sample on inhibiting the secretion of TNF-alpha by comparing the content difference of TNF-alpha secreted by RAW264.7 cells after the administration of the negative control and the test sample. The TNF-alpha content determination adopts an enzyme-linked immunosorbent assay (ELISA) specific principle as follows: after TNF-alpha is specifically combined with a TNF-alpha antibody coated on an enzyme label plate, an anti-TNF-alpha antibody with a substrate label is combined, a colored product is generated after the substrate is catalyzed, and the content of TNF-alpha and the colored product are measured under the wavelength of 450nm to calculate the content of TNF-alpha.

The research method comprises the following steps:

1. cell: the mouse mononuclear macrophage leukemia cell line RAW264.7 is derived from Kunming cell bank of typical culture preservation committee of China academy of sciences, and the model is KCB 200603YJ.

2. The culture conditions are as follows: DMEM medium, fetal bovine serum, trypsin/EDTA solution (0.25% trypsin solution mixed with 0.02mol/L EDTA solution 1).

3. Stimulus: bacterial lipopolysaccharide (LPS Escherichia coli source)

4. Positive control: dexamethasone (Sorbao D8040)

5. Materials, instruments: a 96-well plate; TNF-alpha ELISA detection kit (

ELISA kits); CCK-8 cytotoxicity detection kit (solibao CA 1210); an enzyme-labeling instrument; an ultra-clean bench; dimethyl sulfoxide (DMSO).

6. Study samples: see Table 10

TABLE 10 evaluation of sample grouping information

7. The operation steps are as follows: lipopolysaccharide-induced macrophages RAW264.7 test method (T/SHRH 033-2020) were tested according to the group standard "test of cosmetic soothing efficacy-in vitro TNF-alpha inflammatory factor content".

TNF-alpha content and inhibition calculation

(1) TNF-alpha content

Preparing a standard TNF-alpha standard into a standard solution, diluting the standard solution into a series of solutions with known concentrations in a step-by-step mode according to the proportion, measuring the OD value (OD 450) of the solution at 450nm by using the ELISA method, fitting to prepare a regression equation of a standard curve, carrying the equation on the OD450 value of each test sample, and calculating the content result of the TNF-alpha in the test sample. Each group was tested in duplicate for 3 wells and the mean and Standard Deviation (SD) calculated. SPSS statistical software is used for carrying out t-test analysis on the TNF-alpha content of each sample group and the negative control group, and the difference with P less than 0.05 has statistical significance.

(2) TNF-alpha inhibition rate

Inhibition (%) = (1-T/C) × 100%, where T is the average value of TNF-alpha content in the sample group, and C is the average value of TNF-alpha content in the negative control group.

The research results are as follows:

TABLE 11 CCK-8 cell viability comparison of different dose test samples against inflammatory cells (RAW 264.7)

Table 11 compares CCK-8 cell viability evaluation of purslane extract, panax notoginseng extract, and PHL preservative on inflammatory cells of the macrophage cell line (RAW 264.7), both conventional and pre-formulated. The result shows that the 1, 3-propylene glycol of biological source is used as solvent, the pre-prepared purslane extract and the pseudo-ginseng extract of the PHL preservative system are pre-prepared, and the cell activity of the PHL per se can be kept more than 90% within the concentration range of 0.4-3.2 mg/ml, and the cell activity does not obviously fluctuate with the increase of the dosage. Compared with the traditional purslane extract and the panax notoginseng extract which take the conventional propylene glycol as the solvent, the cell viability of the traditional purslane extract and the panax notoginseng extract is less than 90 percent under the same test condition, and the cell viability is gradually reduced along with the increase of the dosage. The conventional propylene glycol as a solvent is proved to have certain irritation on RAW264.7 cells, so that the cell activity of the RAW264.7 cells is reduced. In contrast, 1, 3-propanediol of biological origin is taken as a solvent, and after the PHL preservative system is prepared, the RAW264.7 cells are not stimulated, and the cell viability is not influenced.

TABLE 12 comparison of inhibition of LPS-induced secretion of TNF- α, an inflammatory factor, from RAW264.7 cells

". Indicates that the test group has a statistical significance for the difference in TNF- α content compared to the negative control group by t-test (P < 0.01);

"#" indicates that the difference of the inhibition rate of the extract of notoginseng using 1, 3-propanediol of biological origin as solvent to TNF-alpha by t-test has statistical significance (P < 0.01) compared with the conventional propanediol as solvent.

Table 12 shows that, under the conditions of this test example, the purslane extract or notoginseng extract, whether in a conventional or pre-formulated form, has a significant inhibitory effect on the secretion of inflammatory factor TNF- α by RAW264.7 cells induced by LPS. Wherein, the inhibition rate of the pre-prepared notoginseng extract on TNF-alpha is obviously higher than that of the conventional notoginseng extract.

And (4) conclusion: under the conditions of the test example, the purslane extract and the panax notoginseng extract both have obvious effect of inhibiting the release of inflammatory factor TNF-alpha. Because 1, 3-propylene glycol of biological source is adopted as a solvent, and the prepared purslane extract and the panax notoginseng extract of the PHL preservative system have lower cytotoxicity, the skin burden can be reduced, and the purslane and panax notoginseng preservative are suitable for SS crowds to be used as daily skin care products for a long time.

Test example 5 evaluation of inhibitory Effect of moisturizing lotion and cream on secretion of three inflammatory factors into RAW264.7 cells induced by LPS

The evaluation study is carried out by the scientific and technological achievement hatching center of Kunming medical university, and the evaluation is carried out according to the section of '6.3 PS induced RAW264.7 cell model anti-inflammatory evaluation' in the 'safety/efficacy evaluation standard of efficacy skin care products for soothing and sensitizing (T/CNMIA 0013-2020)'.

The research purpose is as follows: the soothing effect of the test cosmetic is evaluated by applying an anti-inflammatory effect model of Lipopolysaccharide (LPS) to induce macrophage inflammatory cells (RAW 264.7) to secrete contents of inflammatory factors TNF-alpha, IL-1 beta and IL-6.

The principle is as follows: RAW264.7 is induced by LPS, a classical cell model for the study of inflammatory factors. LPS is combined with macrophage surface antigen recognition receptor, and can induce macrophage to secrete TNF-alpha, IL-1 beta, IL-6 and other inflammatory factors.

TNF-alpha can activate three signal paths of Caspase protease, JNK and transcription factor NF-kB, and realize the biological functions of cytotoxicity, antivirus, immunoregulation, apoptosis and the like.

IL-1 β is an important mediator of inflammatory responses and may be involved in a variety of cellular activities, including cell proliferation, differentiation, apoptosis, etc., when the body is in an inflammatory state or an immune response occurs. IL-1 beta can also cause increase of acute phase reaction protein such as C reaction protein and the like by means of activating endothelial cells, causing bone marrow neutrophil mobilization (leukocytosis), activating various leukocytes and the like, thereby causing systemic inflammation.

IL-6 is produced in different inflammatory patterns. In the early stages of infectious inflammation, different pathogens stimulate the production of IL-6 by monocytes and macrophages through Toll-like receptors (TLRs) of related molecular patterns (PAMPs). In non-infectious inflammatory conditions (e.g., burns or traumatic injuries), IL-6 production by damaged cells is stimulated by damage to a TLR associated molecular pattern (DAMP). This acute IL-6 expression plays a central role in host defense by stimulating various cell populations.

The method evaluates the effect of the test sample on inhibiting the secretion of TNF-alpha, IL-1 beta and IL-6 by comparing the content difference of TNF-alpha, IL-1 beta and IL-6 secreted by RAW264.7 cells after the administration of the negative control and the test sample. The content determination adopts an enzyme-linked immunosorbent assay (ELISA), and the specific principle is as follows: after the inflammatory factor (TNF-alpha, IL-1 beta or IL-6) is specifically combined with the inflammatory factor antibody coated on the enzyme label plate, the anti-inflammatory factor antibody with a substrate label is combined, a colored product is generated after the substrate is catalyzed, and the content of the inflammatory factor and the colored product are measured at the wavelength of 450nm to calculate the content of the inflammatory factor.

The research method comprises the following steps:

3. Stimulus: bacterial lipopolysaccharide (LPS Escherichia coli source)

4. Positive control: dexamethasone (Solaibao D8040)

5. Materials, instruments: a 96-well plate; TNF-alpha ELISA detection kit, IL-1 beta ELISA detection kit, IL-6ELISA detection kit: (

6. And (3) grouping the tests: see Table 13

Table 13 evaluation of sample grouping information

7. The method comprises the following operation steps: according to community standards. The section of '6.3 PS induced RAW264.7 cell model anti-inflammatory evaluation' in the standards for safety/efficacy evaluation of soothing type efficacy skin care products (T/CNMIA 0013-2020).

TNF-alpha, IL-1 beta and IL-6 content and inhibition calculation

(1) Content (c) of

Preparing TNF-alpha, IL-1 beta and IL-6 standard products into standard solutions, diluting the standard solutions into a series of solutions with known concentration step by step according to the proportion, measuring OD values (OD 450) at 450nm by the ELISA method, fitting to prepare a regression equation of a standard curve, carrying the OD450 value of each test sample with an equation, and calculating the content results of TNF-alpha, IL-1 beta and IL-6 in the test sample. Each group was tested in duplicate for 3 wells and the mean and Standard Deviation (SD) calculated. SPSS statistical software is used for carrying out t-test analysis on the contents of TNF-alpha, IL-1 beta and IL-6 of each sample group and a negative control group, which are measured by the test, and the difference of P less than 0.05 has statistical significance.

(2) TNF-alpha, IL-1 beta and IL-6 inhibition rate

The inhibition rate (%) = (1-T/C) × 100%, wherein T is the average content of TNF-alpha, IL-1 beta or IL-6 in each sample group, and C is the average content of TNF-alpha, IL-1 beta or IL-6 in the negative control group.

The research results are as follows:

in the study, a cell model that LPS induces RAW264.7 cells to secrete three inflammatory factors is adopted, and the anti-inflammatory activity of the moisturizing lotion prepared in example 2 and the anti-inflammatory activity of the repair cream prepared in example 3 are compared with that of domestic and foreign famous cosmetics with alleviative effects. Table 14 shows that, except for the test sample No. 3, which had no inhibitory effect on IL-1. Beta. The test samples No. 1 to No. 4, which had inhibitory effects on TNF-. Alpha., IL-1. Beta. And IL-6 secretion from RAW264.7 cells induced by LPS. FIG. 1 shows a comparison of the inhibition of three inflammatory factors in each test, and the inhibition of TNF- α, IL-1 β and IL-6 by 3.2mg/ml test sample # 1 (moisturizing lotion prepared in example 2) was 47.22%, 53.80% and 54.35%, respectively, and the test products had soothing anti-inflammatory effects. Test sample # 2 (the cream prepared in example 3) at 0.8mg/ml had an inhibitory effect on TNF- α, IL-1 β and IL-6 at 71.45%, 72.27% and 91.23% respectively.

TABLE 14 comparison of the inhibitory Effect of the test samples on LPS-induced secretion of inflammatory factors from RAW264.7 cells

And (4) conclusion: under the test, test sample # 1 (moisturizing lotion prepared in example 2, 3.2 mg/ml) and test sample # 2 (repair cream prepared in example 3, 0.8 mg/ml) all have inhibitory effects on secretion of TNF-alpha, IL-1 beta and IL-6 by RAW264.7 cells induced by LPS, which indicates that the test product has soothing anti-inflammatory effects and meets the efficacy evaluation requirements of soothing type efficacy skin care products.

See fig. 1.

Test example 6 evaluation of skin Condition of test subject before and after 14 days after Combined use of face toilet, moisturizing lotion, and cream

Lactic acid sting test

To verify the effectiveness of the use of the product of the invention, 5 Asian adult test volunteers aged 25-55 years were selected during the 4-month period of 2022 and subjected to the lactate stinging test before and after the use of the product of the invention by the skin test subjects. The nasolabial sulcus of the subject was selected for the test, 50 μ L of a 10% lactic acid solution was dropped on a single-layer filter paper having a diameter of 0.8cm, placed on the nasolabial sulcus of the subject, and the subject was asked for a tingling sensation and observed for skin reaction at 2.5 and 5 minutes, respectively, specifically scored as follows:

TABLE 15 lactic acid sting judgment Standard

The evaluation results are specifically as follows:

TABLE 16 lactic acid stinging judgment test results

Subject number	2.5 minutes	5 minutes	Fraction of lactic acid stinging
				1	0	0	0
2	2	1	3
				3	2	2	4
4	2	1	3
				5	2	2	4

As can be seen from the results of the user's self-assessment, 80% of the selected subjects were sensitive skin.

VISIA experiment

In order to verify the effectiveness of the product, 5 adult test volunteers of Asia 25-55 years old are selected during 4 months of 2022 years, facial skin is cleaned by the facial cleanser provided by the invention, then the moisturizing lotion provided by the invention is applied to the face for nursing, and then the repairing cream provided by the invention is applied to the face for moisturizing and nourishing, wherein the repairing cream is applied to the face once a day in the morning and at night for 14 days. After use, the intelligent skin analyzer was used to score skin characteristics for the facial skin for spots, wrinkles, texture, pores, uv spots, brown spots, red areas, and purple, as compared to other persons of the same age, gender, and skin type.

The results are shown in Table 17.

TABLE 17 VISIA facial assessment results

Item

Subject

1

Subject 2

Subject 3

Subject 4

Subject 5

Speckle

90％

75％

66％

34％

66％

Wrinkle (wrinkle)

29％

50％

89％

70％

34％

Texture of

98％

96％

98％

97％

Pores of skin

54％

20％

10％

89％

37％

Ultraviolet ray spot

97％

81％

69％

78％

90％

Brown color spot

96％

37％

63％

49％

40％

Red color zone

75％

40％

72％

62％

21％

Zizhi (purpurin)

63％

98％

91％

99％

95％

Remarking: percentile scores describe skin characteristic scores (higher score better) compared to other persons of the same age, gender, and skin type of the subject.

The result of the change of the indexes of spots, wrinkles, textures, pores, ultraviolet spots, brown spots, red areas, purpura and the like of the facial skin of a user shows that the product of the invention obviously improves the indexes of the spots, the textures, the ultraviolet spots, the brown spots, the purpura and the like of the skin.

Test example 7 evaluation of safety of human skin by combination of the product of the present invention

1. Purpose(s) to

The safety of the product combination on the skin of a human body is evaluated by the human body trial of the product combination for 28 days, wherein the test subject self-evaluates and the dermatologist observes.

2. Test method

1. Test sample

The facial cleanser prepared by the method of example 1; a moisturizer prepared by the method of example 2; a repair cream prepared by the method of example 3.

2. Subject of the disease

Adult subjects 12 (6 men, 6 women) were selected, meeting the following inclusion criteria:

healthy adults aged 18-35 years;

no serious systemic disease, no immunological disease, no skin disease, non-allergic skin;

the test site has not received skin treatment, cosmetic and other treatments that may affect the outcome of the test;

the subject was not administered hormonal drugs or immunosuppressive drugs for the last month and during the test;

the site of the subject was enrolled in other clinical tests during the last month and the duration of the test;

consent to not use skin care products other than the test product during the test;

the female subject is not in pregnancy or lactation.

3. Application method

The subjects used the facial cleanser, moisturizing lotion and cream prepared according to the present invention for personal skin care in the morning and evening for 28 consecutive days as follows. During this time, the subject no longer used other skin cleansing and care products. Cleaning the face by using the facial cleanser and clear water, and uniformly applying a proper amount of moisturizing lotion on the face to gently massage the face until the moisturizing lotion is absorbed; and thirdly, uniformly applying a proper amount of the repair cream on the face, and gently massaging the face until the repair cream is absorbed.

4. Security assessment

The subjects filled in a use log according to subjective feelings within 28 days of using the product combination, and adverse skin reactions during the period were recorded.

Meanwhile, the dermatologist was asked to observe and evaluate the skin of the subject on days 7, 14 and 28, respectively, to evaluate the adverse reactions of the release, such as skin dryness, desquamation, redness, pimple and the like, and the potential possibility thereof. Upon return visit, the subjects were carefully asked, examined and recorded for any adverse events that occurred during the use of the product combination, including the discovery, timing, treatment and outcome of the adverse events, and a determination was made as to the relationship of the adverse events to the product combination used.

The grading of the adverse skin reactions is judged according to the grading standard of the skin reaction of the human trial test fixed in the technical Specification for cosmetic safety 2015, and is as follows:

TABLE 18 skin reaction analysis and Standard for human trial tests

The severity of adverse events was judged according to the following three criteria:

mild: the subject can tolerate the drug without special treatment, and the normal life of the subject is not affected;

moderate: the subject is difficult to tolerate and needs to stop taking or do special treatment, so that the normal life of the subject is influenced;

and (3) severe degree: preventing the subject from living normally and requiring immediate withdrawal or emergency treatment.

Judgment standard of adverse event and test sample relation:

positively, it is related to: the reaction is stopped when the test sample is used and the reaction time sequence is reasonable, or the reaction is quickly reduced or improved when the test sample is stopped, the reaction is reproduced when the test sample is used again, and meanwhile, the reaction is proved by literature data, and the influence of other mixed factors is eliminated;

probably with regard to: the test sample is used and the reaction time sequence is reasonable, the reaction is stopped when the test sample is stopped, or the reaction is quickly relieved or improved, and the influence of other factors can be basically eliminated;

it may be relevant to: the use of test samples and the occurrence time of the reaction are closely related, but more than one sample causing adverse reaction or other factors cannot be excluded;

may not be relevant: the test sample is used, adverse reactions occur, the time relationship is not close, and the reaction is found to be inconsistent with the known adverse reactions of cosmetics;

to be evaluated: the data is incomplete, the evaluation is carried out after the data is supplemented, or the cause and effect relationship is difficult to be determined, and the evidence is lack of literature data;

the following could not be evaluated: too many missing items make the cause and effect relationship difficult to be determined, and the data cannot be supplemented.

3. Test results

TABLE 19 adverse reaction assessment within 28 days of Subjects using the product combination

4. Conclusion

After 28 consecutive days of skin cleansing and care with the product combination, 12 subjects did not experience discomfort nor did they observe adverse skin reactions.

Test example 8 evaluation of the skin tolerance to lactic acid irritation of subjects using the product combination according to the invention

1. Purpose(s) to

The difference in skin tolerance to lactic acid challenge was evaluated for 12 subjects before and after 14 days using the product combination of the invention.

2. Test method

1. Test sample

2. Test subject

healthy adults aged 18-35 years;

no serious systemic, no immune, no skin disease, non-allergic skin;

the test site has not received skin treatment, cosmetics, and other treatments that may affect the outcome of the test;

the subject was not administered a hormonal agent or immunosuppressive agent for the last month and during the test;

the female subject is not in pregnancy or lactation.

3. Application method

Subjects were divided into two groups of 6 persons each (male 3, female 3). One group was a treatment group, and the subjects used the facial cleanser, moisturizer, and cream prepared according to the present invention for personal skin care in the morning and evening for 14 consecutive days according to the following procedure. During this time, the subject no longer used other skin cleansing and care products. Cleaning the face by using the facial cleanser and clear water, and uniformly applying a proper amount of moisturizing lotion on the face to gently massage the face until the moisturizing lotion is absorbed; and step three, uniformly applying a proper amount of repair cream on the face, and gently massaging the face until the repair cream is absorbed.

4. Lactic acid sting test

The treatment group and the control group of subjects are respectively subjected to lactic acid stabbing pain tests before and after 14 days, and the change of the subjects in response to the lactic acid stabbing pain is evaluated and compared through self control. In the test, the subject should first clean his face with clean water, apply 50 μ L of a single-layer filter paper with a diameter of 0.8cm carrying a 10% lactic acid solution to one side of the nasolabial sulcus of the subject, ask the subject for a tingling sensation and observe the skin reaction at 3 and 5 minutes, respectively, and record the subjective feeling score of the subject's skin. 0 part of non-stabbing pain, 1 part of mild stabbing pain, 2 parts of moderate stabbing pain and 3 parts of severe stabbing pain; lactic acid stabbing pain is indicated when the stabbing pain is not less than 3 points.

3. Test results

TABLE 20 comparison of lactic acid sting scores before and after 28 days for subjects in the treatment and control groups

Of the 6 subjects in the treatment group, 5 (83.3% of all) had a reduced score for lactic sting. None of the 6 subjects in the control group had a significant decrease in lactic acid sting response, but instead had an increase in 3-person score.

TABLE 21 improvement of lactic acid stinging in 28 days of the treated group versus the control group [ n, (%) ]

Since the number of test samples was limited and the diopter change values did not fit a normal distribution, a rank-sum test (Mann-Whitney U test) was performed on the individual samples of the lactate prick score change values for the two groups of subjects, with a result P <0.05, indicating that the difference between the 14-day lactate prick score change in the treatment group using the skin care product combination prepared according to the present invention and the control group using the skin care product alone (without the active ingredients of notoginseng extract, purslane extract, and bletilla striata extract) was statistically significant.

4. Conclusion

The skin cleaning and caring of the product combination can effectively improve the reaction of the skin of a subject to lactic acid prickling after being used for 28 days continuously.

Test example 9 evaluation of facial skin of subjects Using the product combination of the invention

1. Purpose(s) to

The improvement of the physiological state of facial skin on the initial and 28 days of the product combination of the invention was evaluated by measuring the water content of the stratum corneum of facial skin, the TEWL value of the transepidermal water loss of skin, and collecting VISIA images of facial skin.

2. Test method

1. Test sample

The face cleanser prepared by the method of example 1; a moisturizer prepared by the method of example 2; a repair cream prepared by the method of example 3.

2. Subject of the disease

Adult subjects (6 men, 6 women) were selected, meeting the following inclusion criteria:

healthy adults aged 18-35 years;

● No serious systemic disease, no immunological disease, no skin disease, and no allergic skin;

● Consent to not use skin care products other than the test product during the test period;

● The female subject is not in pregnancy or lactation

● (ii) stratum corneum moisture content <60c.u. in a side nip region;

water loss from skin in one side nip area>12g/m ² ·h

3. Application method

The subjects used the facial cleanser, moisturizer, and cream prepared according to the present invention for personal skin care in the morning and evening for 28 consecutive days according to the following procedure. During this time, the subject no longer used other skin cleansing and care products. Cleaning the face by using the facial cleanser and clear water, and uniformly applying a proper amount of moisturizing lotion on the face to gently massage the face until the moisturizing lotion is absorbed; and thirdly, uniformly applying a proper amount of the repair cream on the face, and gently massaging the face until the repair cream is absorbed.

4. Face image analysis (VISIA)

On days 0 and 28 with the product combination, subjects tried to rest for 30 minutes in an environment with a temperature of 21 + -1 deg.C and a relative humidity of 50 + -10% after using a clean water face, and a dermatologist was asked to take facial images with a VISIA skin image analyzer and analyze and compare facial texture and red differentiation values.

5. Moisture content of stratum corneum of facial skin

Subjects after completion of the previous step of facial VISIA image acquisition, the subjects left cheek was subjected to stratum corneum hydration testing using a skin hydration tester (Courage + Khazaka, corneometer CM 825).

6. Facial skin moisture loss through skin (TEWL)

Subjects after completion of the facial skin stratum corneum hydration test of the previous step, stratum corneum hydration test was performed on the right cheek of the subject using a skin water loss test probe (Courage + Khazaka, tewameter TM 300).

7. Statistical analysis

The data obtained were analyzed by processing with SPSS. The data collected from each subject before and after 28 days using the product combination were subjected to the Shapiro-Wilk normal distribution test and the homogeneity of variance test, statistically significant and then to the paired sample t-test, at a scale of a =0.05 and p- <0.05, indicating that the differences were significant.

3. Test results

VISIA skin imaging

Referring to fig. 2, 3

TABLE 22 28 day facial VISIA data records for subjects

As assessed by the dermatologist, subjects showed significant improvement in both texture and red silk areas in VISIA imaging of facial skin before and after 28 days using the product combination.

2. Moisture content of the stratum corneum of facial skin

TABLE 23 28-day cheek skin stratum corneum hydration data record for subjects

See fig. 4.

Data analysis showed that the water content of the stratum corneum of the cheek skin of the subjects increased from 34.7% + -12.0% to 47.5% + -11.4% with a rate of change of 37.0% compared before and after 28 days using the product combination. The difference was statistically significant (p < 0.05) by paired sample t-test.

3. Facial skin moisture loss through skin (TEWL)

TABLE 24 TEWL data record for 28-day cheek skin transdermal water loss in 12 subjects

See fig. 5.

Data analysis showed that TWEL skin moisture loss through the cheek skin of the subjects increased from 19.4 + -5.0 to 16.1 + -5.6 with a-16.6% change rate compared before and after 28 days using the present product combination. The difference was statistically significant (p < 0.05) by paired sample t-test.

4. Conclusion

After the product combination is continuously used for 28 days, multiple physiological indexes of facial skin of a subject are improved, skin sensitivity can be effectively relieved, the water content of stratum corneum is increased, the barrier function of the skin is improved, and the product combination has certain effects of relieving, moisturizing and repairing the skin.

Test example 10 evaluation of cytotoxicity of bletilla striata extract

1. Purpose(s) to

The cytotoxicity of bletilla striata extracts at different concentrations on HaCaT keratinocytes was evaluated by the CCK-8 method.

2. Test method

1. Cell: haCaT keratinocytes, from North Na Biotech Ltd.

CCK-8 assay: cells were seeded into 96-well plates with 200. Mu.l cell suspension per well (8 ten thousand cells per well). Culturing in an incubator for 24h, adding substances to be detected with different concentrations into the culture plate, incubating for 24h, sucking supernatant, discarding, adding 100 μ l culture medium and 10 μ l CCK-8 solution mixture into each well, incubating for 2h, and measuring absorbance value at 450nm with an enzyme-labeling instrument.

3. Test results

See fig. 6.

4. Conclusion

According to the CCK-8 result, the bletilla striata extracts are considered to have the cell viability of more than 90 percent in the concentration range of less than or equal to 5 percent on HaCaT keratinocytes, and do not show obvious cytotoxicity.

Test example 11 evaluation of film Forming Properties of bletilla striata extract

1. Object(s) to

The film-forming properties of the bletilla striata extract on the skin surface were evaluated.

2. Test method

Selecting fresh pigskin with shaved surface hair and uniform texture, and cutting into 4 × 3cm ² And divided into three groups. Three groups of pigskins were treated with 120 μ L each of the test samples (group a: 100% bletilla gum; group B: 20% bletilla gum; group C: deionized water). After being left at room temperature (22-26 ℃ C., relative humidity 40-60%) for 1.5 hours, the specimens were observed under a dissecting mirror and photographed. Subsequently, the surface of the pigskin was treated with activated carbon powder (particle size about 75 μm), and photographed and compared again under a dissecting mirror before and after rinsing with water (without using any detergent).

3. Test results

See fig. 7.

4. Conclusion

Seen from a picture under a dissecting mirror, after the bletilla striata gum is smeared and absorbed, a layer of protective film is formed, the invasion of active carbon powder particles to skin textures can be effectively blocked, and the blocking effect of 100% of the bletilla striata gum group is better than that of 20% of the bletilla striata gum group.

Test example 12 evaluation of moisturizing efficacy of bletilla striata extract in moisturizing lotion

1. Purpose(s) to

The moisturizing efficacy of bletilla striata extracts in the moisturizing lotions and creams prepared according to the present invention was evaluated.

2. Test method

1. Grouping

2. Operation of

3 healthy subjects were selected to cleanse their face with the face cleanser prepared according to the present invention, and the initial values of moisture and oil contents of the stratum corneum of the skin on the face of the subjects were recorded using a skin moisture tester (Corneometer CM 825). Applying a proper amount of moisturizing lotion A (or moisturizing lotion B) prepared according to the invention on the skin of the left side face of a subject, and gently massaging the skin until the skin is absorbed; at the same time, the control sample was applied to the right flank skin using the same amount and application method. In the same manner, the skin moisture content of the skin of the cheek of the subject was measured at 0, 3, 5, 10, 30, 60, 90, 120 and 150 minutes using a skin moisture tester, and the change was observed.

3. Test results

See fig. 8.

Experimental determination shows that after a test subject uses the moisturizing lotion A containing 2% of the bletilla striata extract and the moisturizing lotion B containing 1% of the bletilla striata extract, the moisture content of the skin stratum corneum is increased, and the oil content is relatively reduced, wherein the effect of the moisturizing lotion A can last for 150 minutes, and the effect of the moisturizing lotion B can last for about 90 minutes. In contrast, the skin of the subject without the control sample extracted from bletilla striata returned to the initial stratum corneum moisture/oil content towards the initial value at 45 minutes.

4. Conclusion

The rhizoma bletillae extract in the test sample can effectively prolong the duration of the moisturizing effect of the prepared moisturizing lotion on the skin.

Test example 13 evaluation of repair efficacy of bletilla striata extract in repair cream

1. Purpose(s) to

The effect of bletilla striata extract and of the repair cream prepared according to the invention on cell migration of HaCaT was evaluated by a scratch test.

2. Test method

1. Cell: haCaT keratinocytes, from north nai bio-technology limited.

2. Grouping:

3. operation of

After digestion and resuspension of log phase grown HaCaT cells, they were seeded in 6-well platesPlacing 6 well plates at 37 ℃ C. 5% CO ₂ Culturing in an incubator until the holes are fully filled with cells. And (3) drawing a line from top to bottom in each test hole along the same direction by using the sterile gun head to be vertical to the culture hole, grasping the force and the angle in the drawing process, enabling the line to be a straight line as much as possible and to be consistent in width and width, and finishing the drawing once to avoid repeated drawing. The medium was aspirated off, flushed 2 times with PBS buffer, and the cell debris and cell clumps scraped off during the streaking process were washed away. After PBS was aspirated, four groups were set, each group was provided with three multiple wells, and fresh serum-free DMEM medium of white and extract, repair cream, control sample and blank sample was added, respectively, to allow cells to observe changes in cell migration ability under the stimulation of drug of gradient concentration. The scratch change was recorded in the same field of view at 0 hours and 24 hours using an inverted microscope. Calculating the scratch repair rate, and calculating the formula: scratch repair rate = (scratch width 0 h-scratch width 24 h)/scratch width 0 h.

3. Test results

Fig. 9 and 10 show that the 1% of the bletilla striata extract, the repair cream (containing 1% of the bletilla striata extract) and the control sample without the bletilla striata extract can obviously promote migration of HaCaT cells in the scratch test, and the scratch repair rate of the 1% of the bletilla striata extract and the repair cream (containing 1% of the bletilla striata extract) is significantly higher than that of the control sample without the bletilla striata extract (p is less than 0.05 through t-test).

4. Conclusion

The 1% common bletilla pseudobulb extract and the repair cream (containing the common bletilla pseudobulb extract by 1%) can effectively promote the migration of HaCaT cells in a scratch test and have the effect of promoting the repair of skin injury.

In conclusion, the above-mentioned kit composition, face cleanser, moisturizer or cream can be used for the care of sensitive skin, including cleaning, moisturizing, soothing and repairing.

Claims

1. A skin care product set composition with a soothing effect comprises a face cleanser, a moisturizing lotion and a repair cream, and is characterized in that the face cleanser comprises water, disodium EDTA, a surfactant, a humectant, polyquaternium-10, sodium methyl cocoyl taurate, sodium lauroyl isethionate, citric acid, a preservative, a purslane extract pre-prepared solution and PEG-7 glyceryl cocoate; the moisturizing lotion comprises water, glycerol, butanediol, sodium hyaluronate, betaine, EDTA disodium, panthenol, dipotassium glycyrrhizinate, beta-glucan, acetyl chitosamine, pseudo-ginseng extract pre-prepared liquid, purslane extract pre-prepared liquid, lactobacillus/soybean milk fermentation products, tetrahydro-methyl pyrimidine carboxylic acid, rhizoma bletillae extract, preservative and polyacrylate cross-linked polymer-6; the repair cream comprises water, betaine, butanediol, beta-glucan, dipotassium glycyrrhizinate, disodium EDTA, pseudo-ginseng extract pre-prepared liquid, sodium hyaluronate, cetearyl glucoside/sorbitan olivate, caprylic/capric triglyceride, polydimethylsiloxane, shea butter, jojoba oil, squalane, prinus utilis oil, phytosterol oleate, olive oil ceramide, isononyl isononanoate, tocopherol, purslane extract pre-prepared liquid, bletilla extract, preservative, polyacrylate-13, polyisobutylene, polysorbate-20 and sorbitan isostearate.

2. The kit composition of claim 1, wherein the facial cleanser comprises, by weight, 45 to 65% of water, 0.05 to 0.2% of disodium EDTA, 12 to 18% of a surfactant, 15 to 28% of a humectant, 100.1 to 0.3% of polyquaternium-3%, 2 to 4% of sodium methyl cocoyl taurate, 3 to 5% of sodium lauroyl isethionate, 0.9 to 1.3% of citric acid, 1.0 to 1.4% of a preservative, 0.5 to 1.5% of a purslane extract preformulation, and 1.0 to 2.5% of PEG-7 glyceryl cocoate.

3. The kit composition of claim 1, wherein the moisturizing lotion comprises, by weight, 80 to 90% of water, 3 to 5% of glycerin, 2 to 5% of butylene glycol, 0.12 to 0.24% of sodium hyaluronate, 1 to 3% of betaine, 0.05 to 0.2% of disodium EDTA, 0.1 to 0.5% of panthenol, 0.05 to 0.1% of dipotassium glycyrrhizinate, 0.02 to 0.05% of beta-glucan, 0.5 to 2% of acetyl chitosamine, 2 to 5% of pseudo-ginseng extract pre-formulation solution, 0.5 to 2% of purslane extract pre-formulation solution, 0.5 to 2% of lactobacillus/soybean milk fermentation product, 0.1 to 0.5% of tetrahydro-methylpyrimidine carboxylic acid, 0.2 to 2% of bletilla striata extract, 1.0 to 1.4% of a preservative, and 60.3 to 0.7% of polyacrylate cross-linked polymer.

4. The kit composition of claim 1, wherein the repair cream comprises, by weight, 48 to 71% of water, 1 to 3% of betaine, 2 to 5% of butylene glycol, 0.02 to 0.055% of β -glucan, 0.05 to 0.1% of dipotassium glycyrrhizinate, 0.05 to 0.2% of disodium EDTA, 1 to 6% of notoginseng extract pre-formulation, 0.1 to 0.3% of sodium hyaluronate, 2.5 to 5% of cetearyl glucoside/sorbitan olivate, 2 to 5% of caprylic/capric triglyceride, 1 to 3% of polydimethylsiloxane, 3 to 5% of shea butter, 1 to 2% of jojoba oil, 1 to 4% of squalane, 0.5 to 2% of prinus utilis oil, 0.5 to 2% of phytosterol oleate, 0.5 to 2% of olive oil ceramide, 2 to 5% of isononyl isononanoate, 0.2 to 0.5% of tocopherol, 1 to 3% of hyacinth extract pre-formulation, 0.2 to 2% of parsley extract, 1.0 to 1.4% of preservative, 0.03 to 1.1.1.1.12% of parslane isostearate, 0.02 to 1.12% of polysorbate.

5. A facial cleanser with cleaning and moisturizing effects comprises, by weight, 45-65% of water, 0.05-0.2% of EDTA disodium, 12-18% of a surfactant, 15-28% of a humectant, 100.1-0.3% of polyquaternium, 2-4% of sodium methyl cocoyl taurate, 3-5% of sodium lauroyl isethionate, 0.9-1.3% of citric acid, 1.0-1.4% of a preservative, 0.5-1.5% of a purslane extract preparetion solution and 1.0-2.5% of PEG-7 glyceryl cocoate, wherein the surfactant is sodium cocoyl glycinate, and the humectant is glycerin and hydroxypropyl trimethylammonium chloride hyaluronic acid.

6. A moisturizing lotion with a soothing effect comprises, by weight, 80-90% of water, 3-5% of glycerol, 2-5% of butanediol, 0.12-0.24% of sodium hyaluronate, 1-3% of betaine, 0.05-0.2% of disodium EDTA, 0.1-0.5% of panthenol, 0.05-0.1% of dipotassium glycyrrhizinate, 0.02-0.05% of beta-glucan, 0.5-2% of acetyl chitosamine, 2-5% of pseudo-ginseng extract pre-prepared liquid, 0.5-2% of purslane extract pre-prepared liquid, 0.5-2% of lactobacillus/soybean milk fermentation product, 0.1-0.5% of tetrahydro-methyl pyrimidine carboxylic acid, 0.2-2% of rhizoma bletillae extract, 1.0-1.4% of preservative, and 60.3-0.7% of polyacrylate cross-linked polymer.

7. A repair cream with soothing and moisturizing effects comprises, by weight, 48-71% of water, 1-3% of betaine, 2-5% of butanediol, 0.02-0.055% of beta-glucan, 0.05-0.1% of dipotassium glycyrrhizinate, 0.05-0.2% of disodium EDTA, 1-6% of a notoginseng extract pre-prepared liquid, 0.1-0.3% of sodium hyaluronate, 2.5-5% of cetearyl glucoside/sorbitan olivate oleate, 2-5% of caprylic/capric triglyceride, 1-3% of polydimethylsiloxane, 3-5% of shea butter, 1-2% of jojoba oil, 1-4% of squalane, 0.5-2% of prinus utilis royle oil, 0.5-2% of phytosterol oleate, 0.5-2% of olive oil ceramide, 2-5% of isononyl isononanoate, 0.2-0.5% of tocopherol, 1-3% of a hyacinth bletilla extract pre-prepared liquid, 0.2-2% of a sorbitan ester, 1.4-1.4% of a preservative, 0.03-1.1.1.12% of polysorbate, 0.02-0.12% of polyisobutylene.

8. The kit composition, facial cleanser, moisturizer or cream of any one of claims 1 to 7, wherein the purslane extract is preformulated as an effective purslane concentrate diluted to a concentration with 1, 3-propanediol of biological origin and water.

9. The kit composition, cleanser, moisturizer or cream of any one of claims 1 to 7, wherein the preservative is PHL consisting of caprylhydroxamic acid, 1, 2-hexanediol and 1, 3-propanediol.

10. The kit composition, face cleanser, moisturizer or cream of any of claims 1 to 7, which is used for the care of sensitive skin, including cleaning, moisturizing, soothing and rejuvenating.