CN115251401A - Collagen peptide nutritional supplement composition beneficial to immunity improvement and application thereof - Google Patents
Collagen peptide nutritional supplement composition beneficial to immunity improvement and application thereof Download PDFInfo
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- CN115251401A CN115251401A CN202210687898.9A CN202210687898A CN115251401A CN 115251401 A CN115251401 A CN 115251401A CN 202210687898 A CN202210687898 A CN 202210687898A CN 115251401 A CN115251401 A CN 115251401A
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- 239000000203 mixture Substances 0.000 title claims abstract description 59
- 102000008186 Collagen Human genes 0.000 title claims abstract description 32
- 108010035532 Collagen Proteins 0.000 title claims abstract description 32
- 229920001436 collagen Polymers 0.000 title claims abstract description 32
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 31
- 230000036039 immunity Effects 0.000 title claims abstract description 21
- 235000015872 dietary supplement Nutrition 0.000 title claims abstract description 16
- 230000006872 improvement Effects 0.000 title claims abstract description 9
- 230000009286 beneficial effect Effects 0.000 title claims abstract description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 56
- 210000000988 bone and bone Anatomy 0.000 claims abstract description 41
- 239000004310 lactic acid Substances 0.000 claims abstract description 28
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 28
- 239000004365 Protease Substances 0.000 claims abstract description 21
- 240000000691 Houttuynia cordata Species 0.000 claims abstract description 17
- 108091005804 Peptidases Proteins 0.000 claims abstract description 13
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 13
- 244000037364 Cinnamomum aromaticum Species 0.000 claims abstract description 12
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 240000008866 Ziziphus nummularia Species 0.000 claims abstract description 7
- 239000002904 solvent Substances 0.000 claims abstract description 7
- 241000758794 Asarum Species 0.000 claims abstract description 4
- 235000013717 Houttuynia Nutrition 0.000 claims abstract description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 33
- 238000000498 ball milling Methods 0.000 claims description 33
- 229910052791 calcium Inorganic materials 0.000 claims description 33
- 239000011575 calcium Substances 0.000 claims description 33
- 239000000843 powder Substances 0.000 claims description 28
- 239000007788 liquid Substances 0.000 claims description 26
- 210000000582 semen Anatomy 0.000 claims description 25
- 239000012295 chemical reaction liquid Substances 0.000 claims description 21
- 238000000605 extraction Methods 0.000 claims description 20
- 238000000926 separation method Methods 0.000 claims description 18
- 238000007710 freezing Methods 0.000 claims description 16
- 230000008014 freezing Effects 0.000 claims description 16
- 238000006243 chemical reaction Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000002245 particle Substances 0.000 claims description 14
- 239000000047 product Substances 0.000 claims description 14
- 235000013719 Houttuynia cordata Nutrition 0.000 claims description 13
- 239000006228 supernatant Substances 0.000 claims description 13
- 229940036811 bone meal Drugs 0.000 claims description 12
- 239000002374 bone meal Substances 0.000 claims description 12
- 235000019419 proteases Nutrition 0.000 claims description 12
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 claims description 10
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 108090000526 Papain Proteins 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- 238000000227 grinding Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 229940055729 papain Drugs 0.000 claims description 8
- 235000019834 papain Nutrition 0.000 claims description 8
- 239000012264 purified product Substances 0.000 claims description 8
- 238000009835 boiling Methods 0.000 claims description 7
- 239000002552 dosage form Substances 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 230000009849 deactivation Effects 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 229940079593 drug Drugs 0.000 claims description 4
- 239000008187 granular material Substances 0.000 claims description 3
- 239000007902 hard capsule Substances 0.000 claims description 3
- 230000036541 health Effects 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 239000007901 soft capsule Substances 0.000 claims description 3
- 239000003826 tablet Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 230000002708 enhancing effect Effects 0.000 claims 8
- 102000004196 processed proteins & peptides Human genes 0.000 claims 5
- 239000002131 composite material Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 241000411851 herbal medicine Species 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 description 13
- 230000000694 effects Effects 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 238000010926 purge Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
- A23L29/035—Organic compounds containing oxygen as heteroatom
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A collagen peptide nutritional supplement composition beneficial to immunity improvement and application thereof are prepared from the following raw materials in parts by mass: bone: 100-120 parts; heartleaf houttuynia herb: 20-30 parts; wild jujube seed: 5-10 parts; and (2) cassia seed: 15-20 parts of a solvent; protease: 25-30 parts; lactic acid: 30-40 parts. The collagen peptide raw materials are also bones, various other Chinese herbal medicines are compounded, and the collagen peptide raw materials are subjected to enzymolysis, so that all obtained collagen peptide components are micromolecule components, absorption of a user is facilitated, and immunity of users is improved better.
Description
Technical Field
The application relates to a collagen peptide nutritional supplement composition beneficial to immunity improvement and application thereof.
Background
The collagen peptide is a product which is produced by taking fresh animal tissues (including skins, bones, tendons, scales and the like) rich in collagen as raw materials through extraction, hydrolysis and refining and has the relative molecular mass of less than 10000 Da. The existing collagen peptide is mostly used as a health care product, has more benefits for human bodies, and has no main effect due to wide coverage. At present, the effects of promoting absorption of various substances and functional composition are achieved by adopting a mode of compounding various substances, the compounding effect is not good, and the added substances are raw materials, so that the collagen peptide is not suitable for being directly combined with the collagen peptide, and the dosage form mass production of health-care products or medicines in the later period is not facilitated.
Disclosure of Invention
In order to solve the problems, the application discloses a collagen peptide nutritional supplement composition beneficial to immunity improvement, which is prepared from the following raw materials in parts by mass: bone: 100-120 parts; houttuynia cordata: 20-30 parts of a solvent; and (2) spina date seed: 5-10 parts; cassia seed: 15-20 parts of a solvent; protease: 25-30 parts; lactic acid: 30-40 parts. The collagen peptide raw materials are also bones, various other Chinese herbal medicines are compounded, and the collagen peptide raw materials are subjected to enzymolysis, so that all obtained collagen peptide components are micromolecule components, absorption of a user is facilitated, and immunity of users is improved better.
Preferably, the bone, the houttuynia cordata, the spina date seed and the cassia seed are ground into powder, and the particle size is 500-2000 meshes.
Preferably, the synthesis method comprises the following steps:
grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder;
cleaning bone, freezing for later use, and pulverizing frozen bone to obtain bone powder;
mixing powdered herba Houttuyniae, semen Ziziphi Spinosae, semen Cassiae, bone powder, protease and water, adding lactic acid to adjust pH to 5-6, and performing enzymolysis to obtain enzymolysis solution;
adding lactic acid into the enzymolysis liquid, and performing calcium extraction reaction to obtain reaction liquid;
the reaction solution was filtered and dried to obtain a composition. The lactic acid can not only adjust the pH, but also pretreat calcium.
Preferably, the protease is papain; the enzymolysis time is not less than 2h.
Preferably, the freezing is preservation for not less than 3h at-15 to-10 ℃; the enzymolysis temperature is 60-65 deg.C, and the enzyme deactivation time is not less than 5min.
Preferably, the purification is performed as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant to obtain a purified product, wherein the rotational speed of the centrifugal separation is 6000-8000r/min.
Preferably, the calcium extraction is performed by a reaction extraction under a ball milling operation.
Preferably, the ball milling operation is carried out in a planetary high-energy ball mill, the ball milling time is not less than 30min, and the waiting time after ball milling is not less than 120min. The ball milling is adopted in the method for separating calcium from lactic acid, and the calcium is extracted by lactic acid, so that the emulsification effect of secondary macromolecules in a system can be improved, and components with poor water-solubility effect can be mixed into the system instead of being separated out of the system in the next separation.
Preferably, the composition is used for preparing a clinically acceptable dosage form; the acceptable dosage forms comprise powder, granules, tablets, hard capsules, soft capsules and oral liquid.
On the other hand, the application also discloses application of the collagen peptide nutritional supplement composition beneficial to immunity improvement in preparation of health-care products or medicines with improved immunity.
This application can bring following beneficial effect:
1. the collagen peptide raw material, namely bone, is compounded with a plurality of other Chinese herbal medicines, and the collagen peptide raw material is subjected to enzymolysis, so that all obtained components are micromolecule components, and the collagen peptide is easy to be absorbed by a user, and the immunity of the user is better improved;
2. the lactic acid can not only adjust the pH, but also pretreat calcium;
3. the ball milling is adopted in the method for separating calcium from lactic acid, and the calcium is extracted by lactic acid, so that the emulsification effect of secondary macromolecules in a system can be improved, and components with poor water-solubility effect can be mixed into the system instead of being separated out of the system in the next separation.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present application will be explained in detail through the following embodiments.
S1, cleaning ox bones and freezing for later use;
the freezing is to preserve for not less than 3h at the temperature of minus 15 to minus 10 ℃;
s2, crushing the frozen ox bone to obtain animal bone powder; the particle size of the animal bone powder is 500-2000 meshes;
grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder with particle size of 500-2000 mesh;
s3, mixing powdery houttuynia cordata, spina date seeds, semen cassiae, bone meal, protease and water, adding part of lactic acid to adjust the pH to 5-6, and performing enzymolysis to obtain an enzymolysis liquid; wherein the bone: 100-120 parts; and (3) papain: 25-30 parts; houttuynia cordata: 20-30 parts of a solvent; wild jujube seed: 5-10 parts; cassia seed: 15-20 parts of a solvent;
carrying out enzymolysis at 60-65 deg.C for not less than 2 hr to obtain reaction solution, and inactivating enzyme with boiling water for not less than 5min;
s4, adding the residual lactic acid into the enzymatic hydrolysate, and performing calcium extraction to obtain a reaction solution; the total amount of the final lactic acid needs to reach 30-40 parts;
the calcium extraction is carried out under the ball milling operation, the ball milling operation is carried out in a planetary high-energy ball mill, the ball milling time is not less than 30min, and the waiting time after ball milling is not less than 120min;
and S5, purifying and drying the reaction liquid to obtain the composition.
The purification is carried out as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant to obtain a purified product, wherein the rotational speed of the centrifugal separation is 6000-8000r/min.
To further illustrate the process of obtaining the product and the characteristics of the product, the following tests were performed:
example 1:
s1, cleaning ox bones and freezing for later use;
the freezing is to preserve for 3h at-15 ℃;
s2, crushing the frozen ox bone to obtain animal bone powder; the particle size of the animal bone powder is 500-2000 meshes; grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder with particle size of 500-2000 mesh;
s3, mixing powdery houttuynia cordata, spina date seeds, semen cassiae, bone meal, protease and water, adding part of lactic acid to adjust the pH to 5, and performing enzymolysis to obtain an enzymolysis liquid; wherein the bone meal: 1000g; papain: 250g of the total weight of the mixture; heartleaf houttuynia herb: 200g; wild jujube seed: 50g; and (2) cassia seed: 150g of the total weight of the mixture;
carrying out enzymolysis at 60 ℃ for 3h to obtain a reaction solution, and then carrying out enzyme deactivation with boiling water for 5min;
s4, adding the residual lactic acid into the enzymatic hydrolysate, and performing calcium extraction to obtain a reaction solution; the total amount of the final lactic acid needs to reach 30g;
the calcium extraction is carried out by reaction extraction under ball milling operation, the ball milling operation is carried out in a planetary high-energy ball mill, the ball milling time is 30min, and the waiting time is 140min after ball milling;
and S5, purifying and drying the reaction liquid to obtain the composition.
Half of the S4 product was withdrawn and the purge was performed as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant to obtain a purified product, wherein the rotational speed of the centrifugal separation is 6000r/min. Composition 1 was obtained.
The other half was purified and dried to obtain a composition, and the total amount W1 of calcium in the obtained composition 1 and the total amount W2 of calcium in the residue after the supernatant liquid (clear liquid) was taken out were measured, and W1/W2 was 0.13.
Example 2:
s1, cleaning ox bones and freezing for later use;
the freezing step is to store the mixture for 4 hours at the temperature of minus 10 ℃;
s2, crushing the frozen ox bone to obtain animal bone powder; the particle size of the animal bone powder is 500-2000 meshes; grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder with particle size of 500-2000 mesh;
s3, mixing powdery houttuynia cordata, spina date seeds, semen cassiae, bone meal, protease and water, adding part of lactic acid to adjust the pH to 5-6, and performing enzymolysis to obtain an enzymolysis liquid; wherein the bone meal: 1200g; papain: 300g of the total weight of the mixture; houttuynia cordata: 300g of the total weight of the mixture; wild jujube seed: 100g of the total weight of the feed; and (2) cassia seed: 200g of the total weight of the mixture;
carrying out enzymolysis at 65 deg.C for 2h to obtain reaction solution, and inactivating enzyme with boiling water for 10min;
s4, adding the residual lactic acid into the enzymolysis liquid, and performing calcium extraction to obtain a reaction liquid; the total amount of the final lactic acid needs to reach 40g;
the calcium extraction is carried out under the ball milling operation, the ball milling operation is carried out in a planetary high-energy ball mill, the ball milling time is 40min, and the waiting time is 120min after ball milling;
and S5, purifying and drying the reaction liquid to obtain the composition.
Half of the S4 product was removed and the purge was performed as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant liquid to obtain a purified product, wherein the rotational speed of the centrifugal separation is 8000r/min. Composition 2 was obtained.
The other half was purified and dried to obtain a composition, and the total amount W1 of calcium in the obtained composition 2 and the total amount W2 of calcium in the residue after the supernatant liquid (clear liquid) was taken out were measured, and W1/W2 was 0.11.
Example 3:
s1, cleaning ox bones and freezing for later use;
the freezing step is to store the mixture for 3.5 hours at the temperature of-12 ℃;
s2, crushing the frozen ox bone to obtain animal bone powder; the particle size of the animal bone powder is 500-2000 meshes; grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder with particle size of 500-2000 mesh;
s3, mixing powdery houttuynia cordata, spina date seeds, cassia seeds, bone meal, protease and water, adding part of lactic acid to adjust the pH to 5, and performing enzymolysis to obtain an enzymolysis solution; wherein the bone meal: 1100g; and (3) papain: 280g; heartleaf houttuynia herb: 250g of the total weight of the mixture; and (2) spina date seed: 80g of the total weight of the mixture; cassia seed: 180g;
carrying out enzymolysis at 62 ℃ for 3h to obtain a reaction solution, and then carrying out enzyme deactivation with boiling water for 10min;
s4, adding the residual lactic acid into the enzymolysis liquid, and performing calcium extraction to obtain a reaction liquid; the total amount of the final lactic acid needs to reach 35g;
the calcium extraction is carried out under the ball milling operation, the ball milling operation is carried out in a planetary high-energy ball mill, the ball milling time is 35min, and the waiting time is 130min after the ball milling;
and S5, purifying and drying the reaction liquid to obtain the composition.
Half of the S4 product was withdrawn and the purge was performed as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant liquid to obtain a purified product, wherein the rotational speed of the centrifugal separation is 7000r/min. Composition 3 was obtained.
The other half was purified and dried to obtain a composition, and the total amount W1 of calcium in the obtained composition 3 and the total amount W2 of calcium in the residue after the supernatant liquid (clear liquid) was taken out were measured, and W1/W2 was 0.11.
Comparative example 1:
s1, cleaning ox bones and freezing for later use;
the freezing step is to store the mixture for 3.5 hours at the temperature of-12 ℃;
s2, crushing the frozen ox bone to obtain animal bone powder; the particle size of the animal bone powder is 500-2000 meshes; grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder with particle size of 500-2000 mesh;
s3, mixing powdery houttuynia cordata, spina date seeds, semen cassiae, bone meal, protease and water, adding part of lactic acid to adjust the pH to 5, and performing enzymolysis to obtain an enzymolysis liquid; wherein the bone meal: 1100g; and (3) papain: 280g; houttuynia cordata: 250g of the total weight of the mixture; wild jujube seed: 80g of the total weight of the mixture; and (2) cassia seed: 180g;
carrying out enzymolysis at 62 ℃ for 3h to obtain a reaction solution, and then carrying out enzyme deactivation with boiling water for 10min;
s4, calcium extraction: performing reaction extraction under ball milling operation, wherein the ball milling operation is performed in a planetary high-energy ball mill for 35min, and the waiting time is 130min after ball milling;
and S5, purifying and drying the reaction liquid to obtain the composition.
Half of the S4 product was withdrawn and the purge was performed as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant liquid to obtain a purified product, wherein the rotational speed of the centrifugal separation is 7000r/min. Comparative 1 was obtained.
The other half was purified and dried to obtain a composition, and the total amount W1 of calcium in the resulting comparative sample 1 and the total amount W2 of calcium in the residue after the supernatant liquid (clear liquid) was taken out were measured, and W1/W2 was 0.02.
Comparative example 2:
s1, cleaning ox bones and freezing for later use;
the freezing is to preserve for 3.5h at-12 ℃;
s2, crushing the frozen ox bone to obtain animal bone powder; the particle size of the animal bone powder is 500-2000 meshes; grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder with particle size of 500-2000 mesh;
s3, mixing powdery houttuynia cordata, spina date seeds, semen cassiae, bone meal, protease and water, adding dilute hydrochloric acid to adjust the pH value to 5, and performing enzymolysis to obtain an enzymolysis liquid; wherein the bone meal: 1100g; papain: 280g; houttuynia cordata: 250g of the total weight of the mixture; wild jujube seed: 80g of the total weight of the mixture; and (2) cassia seed: 180g;
carrying out enzymolysis at 62 ℃ for 3h to obtain a reaction solution, and then carrying out enzyme deactivation with boiling water for 10min;
s4, adding 35g of lactic acid into the enzymolysis liquid, and performing calcium extraction to obtain a reaction liquid;
the calcium extraction is carried out under the ball milling operation, the ball milling operation is carried out in a planetary high-energy ball mill, the ball milling time is 35min, and the waiting time is 130min after the ball milling;
and S5, purifying and drying the reaction liquid to obtain the composition.
Half of the S4 product was withdrawn and the purge was performed as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant to obtain a purified product, wherein the rotational speed of the centrifugal separation is 7000r/min. Comparative example 2 was obtained.
The other half was purified and dried to obtain a composition, and the total amount W1 of calcium in the resulting comparative sample 2 and the total amount W2 of calcium in the residue after the supernatant liquid (clear liquid) was taken out were measured, and W1/W2 was 0.08.
For the improvement of immunity, the following method is used for detection:
60 test mice were purchased and, 7 days after acclimation, randomly divided into 6 groups of 10 mice each. Labeled as composition 1, composition 2, composition 3, control 1, control 2, and blank, respectively. Then the culture was performed for one month.
For the first 5 groups, gavage was performed at 50mg/kg body weight daily, and other culture conditions were identical. After time is reached, blood is taken for measurement of the number of white blood cells, red blood cells, hemoglobin and lymphocytes. The following results were obtained:
the application is finally substantially the composition of calcium, collagen peptide, traditional Chinese medicine extract and partial residual lactic acid, so the composition can be prepared into clinically acceptable dosage forms; the acceptable dosage forms comprise powder, granules, tablets, hard capsules, soft capsules and oral liquid.
The product obtained by the application contains calcium, collagen peptide and traditional Chinese medicine extract, so the application can be used in medicines or health products for improving the immunity of individuals.
The above are merely examples of the present application and are not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.
Claims (10)
1. A collagen peptide nutritional supplement composition beneficial to immunity improvement is characterized in that: the composite material is prepared from the following raw materials in parts by mass: bone: 100-120 parts; houttuynia cordata: 20-30 parts of a solvent; wild jujube seed: 5-10 parts; cassia seed: 15-20 parts of a solvent; protease: 25-30 parts; lactic acid: 30-40 parts.
2. The immune enhancing collagen peptide nutritional supplement composition according to claim 1, wherein said composition comprises: the bone, the heartleaf houttuynia herb, the spina date seed and the cassia seed are ground into powder with the particle size of 500-2000 meshes.
3. The immune enhancing collagen peptide nutritional supplement composition according to claim 1, wherein said composition comprises: the compound is synthesized according to the following steps:
grinding herba Houttuyniae, semen Ziziphi Spinosae, and semen Cassiae into powder;
cleaning bone, freezing for later use, and pulverizing the frozen bone to obtain bone powder;
mixing powdered herba Houttuyniae, semen Ziziphi Spinosae, semen Cassiae, bone meal, protease and water, adding lactic acid to adjust pH to 5-6, and performing enzymolysis to obtain enzymolysis solution;
adding lactic acid into the enzymolysis liquid, and performing calcium extraction reaction to obtain reaction liquid;
and filtering and drying the reaction liquid to obtain the composition.
4. The nutritional supplement composition of collagen peptides for enhancing immunity according to claim 3, wherein: the protease is papain; the enzymolysis time is not less than 2h.
5. The nutritional supplement composition of collagen peptides for enhancing immunity according to claim 3, wherein: the freezing is to preserve for not less than 3h at the temperature of minus 15 to minus 10 ℃; the enzymolysis temperature is 60-65 deg.C, and the enzyme deactivation with boiling water is carried out for not less than 5min after reaction liquid is obtained.
6. The nutritional supplement composition of collagen peptides for enhancing immunity according to claim 3, wherein: the purification is carried out as follows: and carrying out centrifugal separation on the reaction liquid, and taking supernatant to obtain a purified product, wherein the rotational speed of the centrifugal separation is 6000-8000r/min.
7. The nutritional supplement composition of collagen peptides for enhancing immunity according to claim 3, wherein: the calcium extraction is carried out by reaction extraction under ball milling operation.
8. The nutritional supplement composition of collagen peptides for enhancing immunity according to claim 7, wherein: the ball milling operation is carried out in a planetary high-energy ball mill, the ball milling time is not less than 30min, and the waiting time after ball milling is not less than 120min.
9. A collagen peptide nutritional supplement composition for enhancing immunity according to any one of claims 1 to 8, wherein: the composition is used for preparing clinically acceptable dosage forms; the acceptable dosage forms comprise powder, granules, tablets, hard capsules, soft capsules and oral liquid.
10. Use of the collagen peptide nutritional supplement composition for improving immunity according to any one of claims 1 to 8 in the preparation of health products or medicines for improving immunity.
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