CN109007847A - A method of supplementation product is prepared using donkey bone collagen and donkey bone polypeptide - Google Patents
A method of supplementation product is prepared using donkey bone collagen and donkey bone polypeptide Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/10—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from hair, feathers, horn, skins, leather, bones, or the like
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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Abstract
The invention discloses a kind of methods for preparing supplementation product using donkey bone collagen and donkey bone polypeptide, belong to milk calcium preparation technical field.It is extracted including donkey bone collagen, donkey bone polypeptide extracts and the preparation of supplementation product；The extraction that collagen and polypeptide are carried out to donkey bone that the present invention passes through science, protein is effectively precipitated by digesting, the condition and hydrolytic process of enzyme digestion reaction, it is easy to operate, and the production cycle shortens, it is combined using flavor protease, papain and trypsase, by the synergistic effect of three kinds of enzymes, has reduced the generation of bitter peptides to the greatest extent；Donkey Bone gillg and polypeptide is made, have effects that blood enriching and yin nourishing, blood-nourishing, can kidney-replenishing, strengthening the bones and muscles, improve physical strength energy, the combination that the present invention passes through donkey bone collagen, donkey bone polypeptide, vitamin and other nutritional ingredients simultaneously, the calcareous of needed by human body is replenished in time, convenient for absorbing, bone density can be increased, improve sclerotin.
Description
Technical field
The present invention relates to milk calcium preparation technical fields more particularly to a kind of utilization donkey bone collagen and donkey bone polypeptide to prepare
The method of supplementation product.
Background technique
With the development of social technology level, the fining in food leads to the loss of a large amount of food ingredients, more next
There is calcium deficiency situation in more infants, and calcium major function is to promote the growth and development of infant, promotes the growth of bone, in advance
Myopia prevention etc. is because of disease caused by insufficiency of intake；Infant is in growth and development stage, and the demand to calcium is very urgent.Existing baby
Follow Up Formula, although the body of infant is difficult to absorb there are the ingredient of calcium, there are problems that replenishing the calcium still old calcium deficiency.
Collagen peptides are mainly contained in donkey bone, thus effect-kidney tonifying enriching yin also identical with donkey-hide gelatin, while again
Because it is bone substance, so the effect of also strengthening muscles and bones.Since donkey-hide gelatin is integration of drinking and medicinal herbs kind as defined in the Ministry of Public Health, remove
It is outer extensively in medical clinical application, it is also eaten by people in daily life health care.Up to now, national quality supervision and inspection is examined
63 donkey-hide gelatin the food enterprises of epidemic disease general bureau approved produce edible donkey-hide gelatin and contain phase with it relative to the extensive application of donkey-hide gelatin
Seem that phase shape is shown in my like the research and development application of the by-product donkey bone of functional component.The manufacturing enterprise for generating donkey bone at present will
Donkey bone is stacked everywhere as waste, pollutes environment, is found by literature search with animal glue class market survey, the research of donkey bone
Exploitation is still in domestic and international blank.
But donkey bone ware nutrient content before is low, and is unfavorable for absorption of human body.
Summary of the invention
The purpose of the present invention is to solve solving the problem of that donkey bone ware nutrient content is low and is unfavorable for absorption of human body,
And a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide proposed, effectively improve donkey bone ware nutrition
Content and be conducive to absorption of human body.
To achieve the goals above, present invention employs following technical solutions:
A method of supplementation product is prepared using donkey bone collagen and donkey bone polypeptide, comprising the following steps:
S1, fresh donkey bone, dilute hydrochloric acid, sodium hydroxide solution, flavor protease, papain, trypsase, carbonic acid are chosen
Calcium, chondroitin sulfate, D-Glucosamine Hydrochloride, talcum powder, soybean oil, vitamin D, powdered vitamin C, aloe powder, bee
The raw materials such as pollen, the concentrated sulfuric acid；
Fresh donkey bone soda acid washes 4-7min in S2, selection S1, takes out after being rinsed with pure water to neutrality after taking-up, is put into dryer
It is taken out after middle drying 1-2h, is put into ball mill and is taken out after ball milling 0.5-1.5h, donkey bone fine powder is made；
S3, it takes the donkey bone fine powder in S2 to be put into blender, the pure water of 800%-1000% is added, donkey bone meal homogenate is made, so
1.0%-1.2% flavor protease, 2.5%-3.0% papain and 1.5%-2.0% tryptose is added in backward donkey bone meal homogenate
Enzyme takes mix products A warming-in-water to 55 DEG C -60 DEG C later, then by mix products A mixed enzymolysis 4-6h, then by enzymolysis product
It is put into centrifuge, centrifuged supernatant is taken to be chromatographed, then chromatographic solution is freeze-dried to obtain donkey bone collagen solid；
S4, donkey bone fine powder in S2 is taken, the pure water of 800%-1000% is added, boils 10-15h after mixing, heating is taken to produce
4.5%-5.0% papain and 2.0%-3.0% trypsase is added after being cooled to room temperature in object, then takes mix products heat temperature raising
8-10h is digested after to 45 DEG C -55 DEG C, takes enzymolysis product to be warming up to 120 DEG C of -140 DEG C of enzyme deactivations, enzyme deactivation product is taken to be ultrasonically treated
After carry out centrifugal treating, be made centrifugation product be donkey bone polypeptide.
Preferably, the ammonia sugar donkey ossein calcium tablet preparation method is to take calcium carbonate 40-50g, and it is pure that 760-950g is added
Water is mixed evenly obtained 5%-10% calcium carbonate homogenate, then D-Glucosamine Hydrochloride 9- is added into calcium carbonate homogenate
13g, chondroitin sulfate 10-12g, the donkey bone collagen solid 25-30g in talcum powder 7-10g and S3, will mix after mixing evenly
It closes product B and is sent into 8-15min in efficient wet mixer-granulator, wet granular is made, then wet granular is placed on 55-65 DEG C of condition
Under, dry 20-30min takes desciccate to be put into fully automatic high-speed tablet press machine, and sheeting products are ammonia sugar donkey ossein calcium tablet.
Preferably, the donkey gelatine calcium soft capsule preparation method is to take calcium carbonate 22-28g, vitamin D 6-10g, soybean
Donkey bone collagen solid 10-15g in oily 60-70g, glycerol 2-4g and S3 is mixed evenly, and adds into said mixture
Enter pure water 1000-1905g and mixing homogenate is made, then mixing homogenate spray drying is obtained into admixed finepowder, into admixed finepowder
Soybean oil is added and suspension is made in glycerol, then gelatin is added into suspension and fills pelleting, will be made after product dry packing
Donkey gelatine calcium soft capsule.
Preferably, the donkey Bone gillg powder, preparation method thereof is to take powdered vitamin C5-7g, aloe powder 2-4g, Bee Pollen
Donkey bone collagen solid 12-18g in 4-6g and S3 is uniformly mixed, and pure water 115-350g is added into mixture and is made mixed
Homogenate is closed, liquor capacity is added into mixing homogenate and stands 15-20min after stirring than the concentrated sulfuric acid for 1%, then mixing is homogenized
It is donkey ossein Gly-His-Lys that product successively, which is made, by centrifuge separation, precipitating, acetone washing 2-3 time, freeze-drying and pasteurization.
Preferably, soda acid is washed first to clean 2-3min with 5% dilute hydrochloric acid solution in the S2, then again with 3% sodium hydroxide
Solution cleans 2-4min.
Preferably, the ball mill rotational speed of ball-mill in the S2 is 1200r/min.
Preferably, the revolving speed being centrifugated in the S4 is 90000-11000r/min, and the time of centrifuge separation is 5-
10min。
Preferably, the freeze-drying is -20~-10 DEG C to be placed in temperature, and pressure is dry 1-2h under 6-8Pa.
Compared with prior art, supplementation product is prepared using donkey bone collagen and donkey bone polypeptide the present invention provides a kind of
Method, have it is following the utility model has the advantages that
(1) protein is effectively precipitated by digesting by the extraction for carrying out collagen and polypeptide to donkey bone of science in the present invention,
The condition and hydrolytic process of enzyme digestion reaction, it is easy to operate, and the production cycle shorten, using flavor protease, papain and
Trypsase combination, by the synergistic effect of three kinds of enzymes, has reduced the generation of bitter peptides to the greatest extent；Donkey Bone gillg and more is made
Peptide has effects that blood enriching and yin nourishing, blood-nourishing, can kidney-replenishing, strengthening the bones and muscles, improve physical strength energy, while the present invention pass through donkey bone
The combination of collagen, donkey bone polypeptide, vitamin and other nutritional ingredients, is replenished in time the calcareous of needed by human body, convenient for inhaling
It receives, bone density can be increased, improve sclerotin；
(2) chinese raw materials of the present invention uses donkey bone, and contains a large amount of collagen peptide in donkey bone, so having identical as donkey-hide gelatin
The effect of, and donkey bone, as bone substance, calcium content is very high, so also having effects that strengthen muscles and bones, and donkey bone digests
TCA-NSI in liquid reaches maximum value with PH increase near PH=7, show can be dissolved in donkey bone enzymolysis liquid it is specific molten
The percentage that nitrogen in agent accounts for total nitrogen is very high, and 85% or more is reached near PH=7, and also demonstrating the enzymolysis product is small point
Sub- peptides, small-molecule peptide not only have good acid-soluble, low-viscosity, good physiological activity and anti-gel formative, and
The decomposition product is digested and assimilated fastly in vivo, and protein utilization is high, adapts to most of human body situation, anaphylaxis will not be caused anti-
It answers, may be directly applied in food.
(3) a variety of enzyme immixtures are used in the present invention, in order to improve hydrolysis result, so taking egg first to donkey bone
White matter denatured pretreatment, makes protein conformation change in a short time, and the non-polar group for keeping it internal is exposed to
The surface of molecule increases hydrolysis position, is conducive to enzymolysis, improves the degree of hydrolysis of reaction, but to the physiology of product itself
Activity does not influence, and due to making jointly so using several enzymes in the actual production process in order to accelerate reaction process
With ensure that can keep most fast speed to be hydrolyzed in certain section PH and temperature range.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.
In the description of the present invention, it is to be understood that, term " on ", "lower", "front", "rear", "left", "right", "top",
The orientation or position of the instructions such as "bottom", "inner", "outside", are merely for convenience of description of the present invention and simplification of the description, rather than indicate
Or imply that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore cannot understand
For limitation of the present invention.
Embodiment 1:
A method of supplementation product is prepared using donkey bone collagen and donkey bone polypeptide, comprising the following steps:
S1, fresh donkey bone, dilute hydrochloric acid, sodium hydroxide solution, flavor protease, papain, trypsase, carbonic acid are chosen
Calcium, chondroitin sulfate, D-Glucosamine Hydrochloride, talcum powder, soybean oil, vitamin D, powdered vitamin C, aloe powder, bee
The raw materials such as pollen, the concentrated sulfuric acid；
Fresh donkey bone soda acid washes 4-7min in S2, selection S1, takes out after being rinsed with pure water to neutrality after taking-up, is put into dryer
It is taken out after middle drying 1-2h, is put into ball mill and is taken out after ball milling 0.5-1.5h, donkey bone fine powder is made；
S3, it takes the donkey bone fine powder in S2 to be put into blender, the pure water of 800%-1000% is added, donkey bone meal homogenate is made, so
1.0%-1.2% flavor protease, 2.5%-3.0% papain and 1.5%-2.0% tryptose is added in backward donkey bone meal homogenate
Enzyme takes mix products A warming-in-water to 55 DEG C -60 DEG C later, then by mix products A mixed enzymolysis 4-6h, then by enzymolysis product
It is put into centrifuge, centrifuged supernatant is taken to be chromatographed, then chromatographic solution is freeze-dried to obtain donkey bone collagen solid；
S4, donkey bone fine powder in S2 is taken, the pure water of 800%-1000% is added, boils 10-15h after mixing, heating is taken to produce
4.5%-5.0% papain and 2.0%-3.0% trypsase is added after being cooled to room temperature in object, then takes mix products heat temperature raising
8-10h is digested after to 45 DEG C -55 DEG C, takes enzymolysis product to be warming up to 120 DEG C of -140 DEG C of enzyme deactivations, enzyme deactivation product is taken to be ultrasonically treated
After carry out centrifugal treating, be made centrifugation product be donkey bone polypeptide.
Ammonia sugar donkey ossein calcium tablet preparation method is to take calcium carbonate 40-50g, and 760-950g pure water is added, and is mixed
Uniformly obtained 5%-10% calcium carbonate homogenate, then D-Glucosamine Hydrochloride 9-13g, chondroitin sulfate is added into calcium carbonate homogenate
Mix products B, is sent by the donkey bone collagen solid 25-30g in plain 10-12g, talcum powder 7-10g and S3 after mixing evenly
Wet granular is made in 8-15min in efficient wet mixer-granulator, then under the conditions of wet granular is placed on 55-65 DEG C, dry 20-
30min takes desciccate to be put into fully automatic high-speed tablet press machine, and sheeting products are ammonia sugar donkey ossein calcium tablet.
Donkey gelatine calcium soft capsule preparation method is to take calcium carbonate 22-28g, vitamin D 6-10g, soybean oil 60-70g, sweet
Donkey bone collagen solid 10-15g in oily 2-4g and S3 is mixed evenly, and pure water is added into said mixture
Mixing homogenate is made in 1000-1905g, then mixing homogenate spray drying is obtained admixed finepowder, and soybean is added into admixed finepowder
Suspension is made in oil and glycerol, then gelatin is added into suspension and fills pelleting, and donkey gelatine calcium will be made after product dry packing
Soft capsule.
Donkey Bone gillg powder, preparation method thereof is to take powdered vitamin C5-7g, aloe powder 2-4g, in Bee Pollen 4-6g and S3
Donkey bone collagen solid 12-18g be uniformly mixed, into mixture be added pure water 115-350g be made mixing homogenate, to
Liquor capacity is added in mixing homogenate and stands 15-20min after stirring than the concentrated sulfuric acid for 1%, then mixing homogenate is successively passed through
It is donkey ossein Gly-His-Lys that product, which is made, in centrifuge separation, precipitating, acetone washing 2-3 time, freeze-drying and pasteurization.
Soda acid is washed first to clean 2-3min with 5% dilute hydrochloric acid solution in S2, then cleans 2- with 3% sodium hydroxide solution again
4min。
Ball mill rotational speed of ball-mill in S2 is 1200r/min.
The revolving speed being centrifugated in S4 is 90000-11000r/min, and the time of centrifuge separation is 5-10min.
Freeze-drying is -20~-10 DEG C to be placed in temperature, and pressure is dry 1-2h under 6-8Pa.
Embodiment 2:
Chinese raw materials of the present invention uses donkey bone, and contains a large amount of collagen peptide in donkey bone, so having identical with donkey-hide gelatin
Effect, and donkey bone, as bone substance, calcium content is very high, so also having effects that strengthen muscles and bones, and donkey bone enzymolysis liquid
In TCA-NSI with PH increase reach maximum value near PH=7, specific solvent can be dissolved in by showing in donkey bone enzymolysis liquid
In nitrogen account for total nitrogen percentage it is very high, 85% or more is reached near PH=7, and also demonstrate the enzymolysis product be small molecule
Peptides, small-molecule peptide not only have good acid-soluble, low-viscosity, good physiological activity and anti-gel formative, and should
Decomposition product is digested and assimilated fastly in vivo, and protein utilization is high, adapts to most of human body situation, anaphylaxis will not be caused anti-
It answers, may be directly applied in food；
A variety of enzyme immixtures are used in the present invention, in order to improve hydrolysis result, so taking albumen qualitative change first to donkey bone
Property pretreatment, so that protein conformation is changed in a short time, the non-polar group for keeping it internal is exposed to molecule
Surface increases hydrolysis position, is conducive to enzymolysis, improves the degree of hydrolysis of reaction, but do not have to the physiological activity of product itself
Have an impact, and due to so using several enzyme collective effects, guaranteeing in the actual production process in order to accelerate reaction process
Most fast speed can be kept to be hydrolyzed in certain section PH and temperature range；
Protein, enzyme is effectively precipitated by digesting by the extraction for carrying out collagen and polypeptide to donkey bone of science in the present invention
The condition and hydrolytic process of reaction are solved, it is easy to operate, and the production cycle shortens, using flavor protease, papain and pancreas
Proteinase combination has reduced the generation of bitter peptides to the greatest extent by the synergistic effect of three kinds of enzymes；Donkey Bone gillg and polypeptide is made,
Have effects that blood enriching and yin nourishing, blood-nourishing, can kidney-replenishing, strengthening the bones and muscles, improve physical strength energy, while the present invention pass through donkey gelatine
The combination of former albumen, donkey bone polypeptide, vitamin and other nutritional ingredients, is replenished in time the calcareous of needed by human body, convenient for absorbing,
Bone density can be increased, improve sclerotin.
Embodiment 3:
Fresh donkey bone is acquired, first cleans 3min with 5% dilute hydrochloric acid solution, then cleans 3min with 3% sodium hydroxide solution again, then
It rinses to neutrality, drying is ground into donkey bone fine powder, 10 times of amount pure water reconciliation pulps is added, then companion enters to be equivalent to donkey bone
1.1% flavor protease of fine powder quality, 2.8% papain and 1.8% trypsase, warming-in-water is to 60 DEG C, mixed enzymolysis 5
Hour, it is centrifugated after enzymatic hydrolysis, takes supernatant, be most freeze-dried after chromatographing afterwards, obtain collagen solids；
Fresh donkey bone is taken, cleaned, crushed, the pure water of 10 times of amounts is then added, mixing water decocts system 12 hours, cooling
It adds and is equivalent to 4.8% papain of donkey bone mass and 2.5% trypsase, be warming up to 50 DEG C and be hydrolyzed 10 hours；Enzyme
It is warming up to 130 DEG C of enzyme deactivations after solution, is ultrasonically treated after enzyme deactivation, the sonification power is 350W, ultrasonic treatment
Time is 12min, is finally centrifuged 25min at 10000r/min, takes supernatant up to donkey bone polypeptide.
For donkey bone as raw material, various nutrients are more balanced, and collagen content is high, and Degree of Enzymatic Hydrolysis is high, enzyme
Solution product is small-molecule peptide, is conducive to absorption of human body and utilization, will not cause allergic reaction.
Embodiment 4:
It takes calcium carbonate 45g to be configured to the calcium carbonate that mass fraction is 10% with pure water to be homogenized, D- is then added thereto again
Aminoglucose hydrochloride 11g, chondroitin sulfate 11g, donkey bone collagen 28g and 8 parts of talcum powder are sent into high after stirring and evenly mixing
It imitates in wet mixing pelletizer, mixes 12min, wet granular is made, then under the conditions of 60 DEG C, dry 25min granulation, most afterwards through complete
Ammonia sugar donkey ossein calcium tablet is made in automatic high speed tabletting machine；
For ammonia sugar donkey ossein calcium tablet using donkey bone as raw material, calcium content itself is higher, and donkey bone collagen is in PH=7
It nearby decomposes most fast, is easily absorbed by the body, is made after calcium tablet, volume is compressed, but nutrient content is very high, not only can be with
A large amount of calcium constituents are provided for human body, a large amount of collagens can also be provided.
Embodiment 5:
Calcium carbonate 25g, vitamin D 8g, soybean oil 65g, donkey bone collagen 12g and glycerol 3g are taken, first by calcium carbonate, donkey gelatine
Mixed serum is made in former protein and vitamins D mixing, the pure water that 12 times of amounts are then added, and be spray-dried obtain mixing it is micro-
Then suspension is made with soybean oil and glycerol again in powder, suspension is being filled pelleting using gelatin, through dry and packaging
Obtain donkey gelatine calcium soft capsule；
Because suspension produced mouthfeel itself is bad and is easy to volatilize, easily decomposed in the oral cavity by saliva, easily sucking tracheae, but
It is that suspension is packed into capsule, both medicine property of medicine was protected not to be destroyed, also protects digestive organs and respiratory tract, and remove
Capsule shells are likely to result in drug wastage, drug waste, drug effect and reduce, in addition, some ingredients need to dissolve and absorb in the intestine, glue
Capsule protects nutritional ingredient not destroyed by gastric acid, is conducive to absorption of human body and utilizes.
Embodiment 6:
Take donkey bone collagen 15g, vitamin c powder 6g, aloe powder 3g, Bee Pollen 5g；It is uniformly mixed, 8 times of amount pure water is added
Mixing homogenate is reconciled into, liquor capacity is then added into mixing homogenate than the concentrated sulfuric acid for 1:100, stands 15min after stirring,
Then it is centrifugated 10min, centrifugal rotational speed 10000r/min again, is used acetone washing 2 times after precipitating, being placed on temperature is -15
DEG C, pressure is to be freeze-dried 1.5h under 7Pa, obtains donkey ossein Gly-His-Lys after finally carrying out pasteurization；
Donkey bone collagen powder, after pulvis is made in the collagen of script, protein denaturation makes it be easier to be digested, and
And it is more easier to be fed by human body.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide, which is characterized in that including following step
It is rapid:
S1, fresh donkey bone, dilute hydrochloric acid, sodium hydroxide solution, flavor protease, papain, trypsase, carbonic acid are chosen
Calcium, chondroitin sulfate, D-Glucosamine Hydrochloride, talcum powder, soybean oil, vitamin D, powdered vitamin C, aloe powder, bee
The raw materials such as pollen, the concentrated sulfuric acid；
Fresh donkey bone soda acid washes 4-7min in S2, selection S1, takes out after being rinsed with pure water to neutrality after taking-up, is put into dryer
It is taken out after middle drying 1-2h, is put into ball mill and is taken out after ball milling 0.5-1.5h, donkey bone fine powder is made；
S3, it takes the donkey bone fine powder in S2 to be put into blender, the pure water of 800%-1000% is added, donkey bone meal homogenate is made, so
1.0%-1.2% flavor protease, 2.5%-3.0% papain and 1.5%-2.0% tryptose is added in backward donkey bone meal homogenate
Enzyme takes mix products A warming-in-water to 55 DEG C -60 DEG C later, then by mix products A mixed enzymolysis 4-6h, then by enzymolysis product
It is put into centrifuge, centrifuged supernatant is taken to be chromatographed, then chromatographic solution is freeze-dried to obtain donkey bone collagen solid；
S4, donkey bone fine powder in S2 is taken, the pure water of 800%-1000% is added, boils 10-15h after mixing, heating is taken to produce
4.5%-5.0% papain and 2.0%-3.0% trypsase is added after being cooled to room temperature in object, then takes mix products heat temperature raising
8-10h is digested after to 45 DEG C -55 DEG C, takes enzymolysis product to be warming up to 120 DEG C of -140 DEG C of enzyme deactivations, enzyme deactivation product is taken to be ultrasonically treated
After carry out centrifugal treating, be made centrifugation product be donkey bone polypeptide.
2. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide according to claim 1,
Be characterized in that: the ammonia sugar donkey ossein calcium tablet preparation method is to take calcium carbonate 40-50g, and 760-950g pure water is added, and is mixed
Conjunction stirs evenly obtained 5%-10% calcium carbonate homogenate, then D-Glucosamine Hydrochloride 9-13g, sulphur is added into calcium carbonate homogenate
Donkey bone collagen solid 25-30g in aching and limp ossein 10-12g, talcum powder 7-10g and S3, after mixing evenly by mix products
B is sent into 8-15min in efficient wet mixer-granulator, wet granular is made, then under the conditions of wet granular is placed on 55-65 DEG C, does
Dry 20-30min takes desciccate to be put into fully automatic high-speed tablet press machine, and sheeting products are ammonia sugar donkey ossein calcium tablet.
3. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide according to claim 1,
Be characterized in that: the donkey gelatine calcium soft capsule preparation method is to take calcium carbonate 22-28g, vitamin D 6-10g, soybean oil 60-
Donkey bone collagen solid 10-15g in 70g, glycerol 2-4g and S3 is mixed evenly, and is added into said mixture pure
Mixing homogenate is made in water 1000-1905g, then mixing homogenate spray drying is obtained admixed finepowder, is added into admixed finepowder big
Suspension is made in soya-bean oil and glycerol, then gelatin is added into suspension and fills pelleting, and donkey gelatine will be made after product dry packing
Calcium soft capsule.
4. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide according to claim 1,
Be characterized in that: the donkey Bone gillg powder, preparation method thereof be take powdered vitamin C5-7g, aloe powder 2-4g, Bee Pollen 4-6g and
Donkey bone collagen solid 12-18g in S3 is uniformly mixed, and it is even that the obtained mixing of pure water 115-350g is added into mixture
Liquor capacity is added into mixing homogenate and stands 15-20min after stirring than the concentrated sulfuric acid for 1% for slurry, then successively by mixing homogenate
It is donkey ossein Gly-His-Lys that product, which is made, by centrifuge separation, precipitating, acetone washing 2-3 times, freeze-drying and pasteurization.
5. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide according to claim 1,
Be characterized in that: soda acid is washed first to clean 2-3min with 5% dilute hydrochloric acid solution in the S2, then clear with 3% sodium hydroxide solution again
Wash 2-4min.
6. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide according to claim 1,
Be characterized in that: the ball mill rotational speed of ball-mill in the S2 is 1200r/min.
7. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide according to claim 1,
Be characterized in that: the revolving speed being centrifugated in the S4 is 90000-11000r/min, and the time of centrifuge separation is 5-10min.
8. a kind of method for preparing supplementation product using donkey bone collagen and donkey bone polypeptide according to claim 4,
Be characterized in that: the freeze-drying is -20~-10 DEG C to be placed in temperature, and pressure is dry 1-2h under 6-8Pa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810762646.1A CN109007847A (en) | 2018-07-12 | 2018-07-12 | A method of supplementation product is prepared using donkey bone collagen and donkey bone polypeptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810762646.1A CN109007847A (en) | 2018-07-12 | 2018-07-12 | A method of supplementation product is prepared using donkey bone collagen and donkey bone polypeptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112812175A (en) * | 2021-02-02 | 2021-05-18 | 江南大学 | Application of high-glutamic acid donkey collagen active peptide |
| CN114468309A (en) * | 2022-02-22 | 2022-05-13 | 山东东阿古胶阿胶系列产品有限公司 | Preparation process of donkey bone chelated calcium peptide bone strengthening tablet and bone strengthening tablet |
| CN115251401A (en) * | 2022-06-17 | 2022-11-01 | 兆鑫堂(山东)生物科技有限公司 | Collagen peptide nutritional supplement composition beneficial to immunity improvement and application thereof |
-
2018
- 2018-07-12 CN CN201810762646.1A patent/CN109007847A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112812175A (en) * | 2021-02-02 | 2021-05-18 | 江南大学 | Application of high-glutamic acid donkey collagen active peptide |
| CN114468309A (en) * | 2022-02-22 | 2022-05-13 | 山东东阿古胶阿胶系列产品有限公司 | Preparation process of donkey bone chelated calcium peptide bone strengthening tablet and bone strengthening tablet |
| CN115251401A (en) * | 2022-06-17 | 2022-11-01 | 兆鑫堂(山东)生物科技有限公司 | Collagen peptide nutritional supplement composition beneficial to immunity improvement and application thereof |
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Inventor after: Pan Yuzhu Inventor after: Dong Jinbao Inventor after: He Tao Inventor before: Dong Jinbao Inventor before: Pan Yuzhu Inventor before: He Tao |
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Application publication date: 20181218 |