CN115250781A - Device and method for manually and rapidly inoculating pine wood nematodes - Google Patents
Device and method for manually and rapidly inoculating pine wood nematodes Download PDFInfo
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- CN115250781A CN115250781A CN202210524673.1A CN202210524673A CN115250781A CN 115250781 A CN115250781 A CN 115250781A CN 202210524673 A CN202210524673 A CN 202210524673A CN 115250781 A CN115250781 A CN 115250781A
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- 241000243770 Bursaphelenchus Species 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims description 21
- 238000011081 inoculation Methods 0.000 claims abstract description 105
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 238000007789 sealing Methods 0.000 claims abstract description 26
- 241000243771 Bursaphelenchus xylophilus Species 0.000 claims abstract description 19
- 241000238631 Hexapoda Species 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 9
- 230000005484 gravity Effects 0.000 claims abstract description 7
- 238000002347 injection Methods 0.000 claims abstract description 7
- 239000007924 injection Substances 0.000 claims abstract description 7
- 238000001704 evaporation Methods 0.000 claims abstract description 6
- 241000018646 Pinus brutia Species 0.000 claims description 18
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims description 17
- 235000011613 Pinus brutia Nutrition 0.000 claims description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005520 cutting process Methods 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 6
- 238000004080 punching Methods 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 10
- 238000002474 experimental method Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000002265 prevention Effects 0.000 abstract description 2
- 241000244206 Nematoda Species 0.000 description 15
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000004083 survival effect Effects 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- 241000422846 Sequoiadendron giganteum Species 0.000 description 3
- 230000009545 invasion Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000218631 Coniferophyta Species 0.000 description 1
- 241001442207 Monochamus alternatus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/40—Afforestation or reforestation
Abstract
The invention discloses a device for manually and quickly inoculating pine wood nematodes, which comprises a inoculation tube, wherein a closed conical tube is arranged at the bottom end of the inoculation tube, a liquid injection port is formed in the top of the inoculation tube, a sealing cover is arranged on the liquid injection port, and an air hole is formed in the sealing cover. According to the invention, the moisture in the inoculation pipe can be prevented from evaporating, the air pressure in the inoculation pipe is consistent with the outside through the air holes, the insect liquid in the inoculation pipe can quickly enter the inoculated branches under the action of gravity, the progress of an experiment is greatly accelerated, a plurality of groups of experimental data can be made in the same time, the acquisition of the experimental data is greatly enriched, in addition, the pine wood nematode is inoculated by adopting the inoculation pipe for the first time, the rain and wind prevention is realized, and the reliability of the inoculation result is ensured; secondly, the invention has simple manufacture, low material price and good effect, and can be more suitable for various woodland inoculation or laboratory pine wood nematode inoculation experiments.
Description
Technical Field
The invention relates to the field of pine wood nematode inoculation, in particular to a device and a method for artificially and quickly inoculating pine wood nematodes.
Background
The pine tree is used as a gymnosperm evergreen arbor, has a tall and big tree body and a tall and ambitious posture, can be used from the trunk to the pine nut and the pine leaf, and has economic value, ecological value and social value. Although pine trees are cold-resistant, drought-resistant, light-loving and moisture-loving, the pine trees are poor in negative resistance and are required to grow in a humid environment with sufficient light and fertile soil. Due to the fast growing nature, pine species are widely planted as the primary replacement species for post-harvest forested land. Pine nematode disease (Pine nematode) is a forestry destructive disease caused by Pine nematodes (Bursaphelenchus xylophilus), and the Pine nematode disease occurs in a plurality of provinces in China at present, so that great loss is brought to forestry and an ecosystem. At present, the technical means for preventing and controlling the pine wood nematode disease mainly comprise punching and injecting pesticide to prevent the invasion of the pine wood nematode, hanging a trap and controlling chemical agents to reduce the density of the monochamus alternatus hope, but the most fundamental prevention and control means for the pine wood nematode disease can produce disease-resistant pine trees as soon as possible. At present, most pine tree nematode inoculation methods adopt a skin grafting method, which comprises the steps of obliquely cutting tender lateral branches of pine trees to xylem by using a scalpel, filling absorbent cotton into an oblique cut wound, manufacturing the pine trees into a funnel shape by using a sealing film, filling insect liquid to be inoculated into the absorbent cotton, and supplementing water once every 12 hours, so that the phenomenon that the nematodes lose activity and cannot enter the wound due to water loss is avoided, and nearly one third of the nematodes in the cotton cannot enter the wound in the actual operation process, so that the actual quantity of inoculation cannot be controlled. The method is complex to operate, and whether the liquid in the funnel-shaped sealing film is spilled in windy and rainy days or not needs to be noticed in field operation. Therefore, the establishment of a method for conveniently and rapidly inoculating the pine wood nematodes has wide application prospect and important production significance.
Disclosure of Invention
The invention aims to overcome the problems in the prior art, and provides a device and a method for manually and quickly inoculating pine wood nematodes, which have the advantages of quickly inoculating large seedlings with pine wood nematodes and reducing subsequent complicated maintenance work.
In order to achieve the technical purpose and achieve the technical effect, the invention is realized by the following technical scheme:
the utility model provides an artifical inoculation pine wood nematode device fast, includes the inoculation pipe, the bottom of inoculation pipe is provided with confined toper pipe, the top of inoculation pipe is provided with annotates the liquid mouth, annotate and install sealed lid on the liquid mouth, sealed covering has seted up the bleeder vent.
The technical scheme is realized, the cut part of the conical pipe is used for accessing the inoculation branch, the liquid injection port is used for injecting the insect liquid into the inoculation pipe, the sealing cover is used for covering the inoculation pipe so as to be used for moisturizing the inoculation pipe and preventing water in the pipe from evaporating, and the air vents enable the air pressure in the inoculation pipe to be consistent with the outside, so that the insect liquid in the inoculation pipe can enter the inoculation branch more quickly under the action of gravity, the progress of an experiment is greatly accelerated, a plurality of groups of experimental data can be made in the same time, the acquisition of the experimental data is greatly enriched, in addition, the inoculation mode of the pine wood nematode is firstly adopted, wind and rain are prevented, and the reliability of the inoculation result is ensured; secondly, the invention has simple manufacture, low material price and good effect, and can be more suitable for various woodland inoculation or laboratory pine wood nematode inoculation experiments.
As a preferable scheme of the invention, the disposable inoculation device further comprises a sticker used for being stuck on the inoculation tube.
The technical scheme is realized, on one hand, the device is used for shielding ultraviolet irradiation, influencing the activity of the nematodes and keeping the survival rate of the nematodes, and on the other hand, the device can be used for recording inoculation information.
In a preferred embodiment of the present invention, the sealing cover and the seed tube are connected by a connecting band.
Realize above-mentioned technical scheme, prevent that sealed lid from losing.
As a preferable scheme of the present invention, the inoculation tube is made of a translucent plastic material.
The technical scheme is realized so as to observe the insect liquid in the inoculation pipe.
As a preferable scheme of the present invention, the sealing cover is made of a non-transparent plastic material for shielding sunlight.
By adopting the technical scheme, the irradiation of sunlight is reduced, and the activity of the nematodes is ensured.
A method for artificially and rapidly inoculating pine wood nematodes comprises the following steps:
step one, selecting tender shoot branches below the tips of pine seedlings for inoculation, and cutting the heads of the inoculated branches flat;
step two, cutting off part of the conical pipe at the bottom of the inoculation pipe to enable the diameter of an opening at the tail end of the conical pipe to be similar to that of the inoculated branch;
step three, sleeving the opening of the conical pipe at the bottom of the inoculation pipe on the inoculation branch, and fixing and sealing the conical pipe and the inoculation branch by using a sealing film to prevent insect liquid from seeping out of the pipe;
injecting insect liquid into the liquid injection port of the inoculation pipe, and covering a sealing cover to prevent moisture in the inoculation pipe from evaporating;
fifthly, punching is carried out on the sealing cover, so that outside air can enter the inoculation pipe, and insect liquid in the inoculation pipe can enter branches rapidly under the action of gravity and enter the tree body through the branches;
and step six, finally, sticking a sticker on the inoculation tube to prevent direct sunlight.
The technical scheme is realized, and the device is used for quickly inoculating insect liquid into branches.
As a preferable scheme of the invention, the number of the pine wood nematodes in the liquid is 3000-5000.
The technical scheme is realized so as to ensure the survival amount of the pine wood nematodes entering the branches.
In a preferred embodiment of the present invention, the head of the inoculated branch is further provided with a streak line.
The technical scheme is realized so that the insect liquid can enter the branches more quickly.
The beneficial effects of the invention are:
1. the device and the method designed by the invention can greatly accelerate the progress of the experiment, so that more groups of experimental data can be made in the same time, and the acquisition of the experimental data is greatly enriched;
2. according to the invention, the pine wood nematode is inoculated by adopting the inoculation tube for the first time, so that wind and rain are prevented, and the reliability of the inoculation result is ensured;
3. the invention has simple manufacture, low material price and good effect, and can be more suitable for various woodland inoculation or laboratory pine wood nematode inoculation experiments.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to be implemented in accordance with the content of the specification, the following detailed description is made of preferred embodiments of the present invention in conjunction with the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and do not limit the invention. In the drawings:
FIG. 1 is a schematic view relating to the present invention;
FIG. 2 is a schematic view of the air hole of the sealing cover according to the present invention;
FIG. 3 is a schematic illustration of an inoculation process according to the present invention.
The reference numbers in the figures illustrate: the inoculation tube 1, the conical tube 2, the liquid injection port 3, the sealing cover 4, the air vent 5, the sticker 6, the connecting belt 7, the branch 8 and the sealing film 9.
Detailed Description
The invention is further described below with reference to the accompanying drawings:
example (b):
the utility model provides an artifical inoculation pine wood nematode device fast, refers to fig. 1, fig. 2 and fig. 3, includes inoculation pipe 1, and the bottom of inoculation pipe 1 is provided with confined conical tube 2, and the top of inoculation pipe 1 is provided with annotates liquid mouth 3, annotates and installs sealed lid 4 on the liquid mouth 3, has seted up bleeder vent 5 on the sealed lid 4. In this embodiment, conical duct 2 and inoculation pipe 1 integrative injection moulding, sealed lid 4 passes through connecting band 7 to be connected in inoculation pipe 1, lose after opening in order to prevent sealed lid 4, inoculation pipe 1 adopts translucent plastics material to make, so that observe the worm liquid in inoculation pipe 1 when using, conical duct 2 cuts the part back and is used for inserting inoculation branch 8, it is used for pouring into worm liquid into inoculation pipe 1 to annotate liquid mouth 3, sealed lid 4 is used for covering on inoculation pipe 1, in order to be used for inoculation pipe 1 to moisturize, prevent the evaporation of water in the pipe, and the atmospheric pressure in the inoculation pipe 1 that the design of bleeder vent 5 made is unanimous with the external world, the worm liquid in the inoculation pipe 1 of messenger can get into inoculation branch 8 under the action of gravity faster.
In this embodiment, sealed lid 4 adopts the non-transparent plastic material who shelters from sunshine to make to reduce shining of sunshine, guarantee the activity of nematode.
In this embodiment, the sticker 6 is adhered to the inoculation tube 1, and the sticker 6 is used for shielding ultraviolet irradiation, influencing the activity of nematodes and ensuring the survival rate of the nematodes on one hand, and can be used for recording inoculation information on the other hand.
The invention also discloses a method for artificially and rapidly inoculating the pine wood nematodes, which comprises the following steps:
step one, selecting tender shoot branches 8 below the tips of pine seedlings for inoculation, and cutting the heads of the inoculated branches 8 flat;
step two, cutting off a part of the conical pipe 2 at the bottom of the inoculation pipe 1 to enable the opening at the tail end of the conical pipe 2 to be similar to the diameter of the inoculation branch 8;
step three, sleeving an opening of the conical pipe 2 at the bottom of the inoculation pipe 1 on the inoculation branch 8, and fixing and sealing the conical pipe 2 and the inoculation branch 8 by using a sealing film 9 to prevent insect liquid from seeping out of the pipe;
step four, injecting insect liquid into the liquid injection port 3 of the inoculation tube 1, and covering a sealing cover 4 to prevent water in the inoculation tube 1 from evaporating;
fifthly, punching is carried out on the sealing cover 4, so that outside air can enter the inoculation pipe 1, and insect liquid in the inoculation pipe 1 can enter the branches 8 more quickly under the action of gravity and enter the tree body through the branches 8;
and step six, finally, sticking stickers on the inoculation tube 1 to prevent direct irradiation of sunlight.
The number of the pine wood nematodes in the insect liquid is set to be 3000-5000 to ensure the survival amount of the pine wood nematodes entering the branches 8, and in addition, the heads of the inoculated branches 8 are also provided with streaks to facilitate the insect liquid to enter the branches 8 more quickly.
The inoculation method adopted by the invention is simple, convenient and quick, firstly, the invention has the moisture-keeping sealing cover 4, so that the moisture is not required to be supplemented every 12 hours, and if the test is carried out on a field forest, the water is not required to be kept beside an inoculated pine all the time, and the water can be directly separated after the inoculation; secondly, due to the action of the sealing cover 4, the inoculation environment is not damaged by rain gusts in summer in the field inoculation experiment process; the method is more convenient and rapid to inoculate the field big tree, and only one annual branch needs to be found on the big tree for inoculation; the inoculation method is protected by the sticker 6, so that the activity of the pine wood nematodes cannot be influenced even if ultraviolet rays of scattered sunlight exist in the forest, and the reality and reliability of experimental data are ensured; finally, due to the existence of the air holes 5, the internal and external air pressures are stable, and the nematodes can be accelerated to invade the wound under the action of gravity. Therefore, the method is quick to operate, extremely low in difficulty in handling, capable of guaranteeing stable invasion of the nematodes and improving the reliability of experimental data.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (8)
1. The utility model provides an artifical inoculation pine wood nematode device fast, its characterized in that, includes inoculation pipe (1), the bottom of inoculation pipe (1) is provided with confined toper pipe (2), the top of inoculation pipe (1) is provided with annotates liquid mouth (3), install sealed lid (4) on annotating liquid mouth (3), bleeder vent (5) have been seted up on sealed lid (4).
2. The artificial rapid pine wood nematode inoculation device according to claim 1, characterized by further comprising a sticker (6) for sticking on the inoculation tube (1).
3. The artificial rapid pine wood nematode inoculation device according to claim 1, wherein the sealing cover (4) and the inoculation tube (1) are connected through a connecting belt (7).
4. The artificial fast pine wood nematode inoculation device according to claim 1, wherein the inoculation tube (1) is made of translucent plastic material.
5. The artificial fast pine wood nematode inoculation device according to claim 1, wherein the sealing cover (4) is made of a non-transparent plastic material which can shield sunlight.
6. A method for artificially and rapidly inoculating pine wood nematodes is characterized by comprising the following steps:
step one, selecting a tender shoot branch (8) below the top of the pine seedling, inoculating, and cutting the head of the inoculated branch (8) flat;
step two, cutting off part of the conical pipe (2) at the bottom of the inoculation pipe (1) to enable the opening at the tail end of the conical pipe (2) to be similar to the diameter of the inoculation branch (8);
step three, sleeving an opening of the conical pipe (2) at the bottom of the inoculation pipe (1) on the inoculation branch (8), and fixing and sealing the conical pipe (2) and the inoculation branch (8) by using a sealing film (9) to prevent insect liquid from leaking out of the pipe;
step four, injecting insect liquid into the liquid injection port (3) of the inoculation tube (1), and covering a sealing cover (4) to prevent water in the inoculation tube (1) from evaporating;
fifthly, punching is carried out on the sealing cover (4), so that outside air can enter the inoculation pipe (1), and insect liquid in the inoculation pipe (1) can enter the branches (8) more quickly under the action of gravity and enter the tree body through the branches (8);
and step six, finally, sticking a sticker (6) on the inoculation tube (1) to prevent direct sunlight.
7. The method as claimed in claim, wherein the number of the pine wood nematodes in the solution is 3000-5000.
8. The method for artificial rapid inoculation of Bursaphelenchus xylophilus according to claim, wherein the head of the inoculated branch (8) is further provided with a streak.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210524673.1A CN115250781A (en) | 2022-05-13 | 2022-05-13 | Device and method for manually and rapidly inoculating pine wood nematodes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210524673.1A CN115250781A (en) | 2022-05-13 | 2022-05-13 | Device and method for manually and rapidly inoculating pine wood nematodes |
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| Publication Number | Publication Date |
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| CN115250781A true CN115250781A (en) | 2022-11-01 |
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| CN202210524673.1A Pending CN115250781A (en) | 2022-05-13 | 2022-05-13 | Device and method for manually and rapidly inoculating pine wood nematodes |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003250337A (en) * | 2002-02-26 | 2003-09-09 | Nara Prefecture | Mycorrhiza organizer and method for forming mycorrhiza |
| CN102726247A (en) * | 2011-04-11 | 2012-10-17 | 乌鲁木齐爱思特专利转让服务有限责任公司 | Seedling raising method |
| US20130224837A1 (en) * | 2012-02-24 | 2013-08-29 | Margret Elaine LEVENTHAL | Inoculation tool and method of use thereof |
| CN103493811A (en) * | 2013-10-21 | 2014-01-08 | 四川农业大学 | Method for prevention and treatment of Bursaphelenchus mucronatus disease |
| CN206933052U (en) * | 2017-06-29 | 2018-01-30 | 北京安和亿泰生物工程技术有限公司 | A kind of nematode Steinernema carpocapsae Propogation and culture ware |
| CN208175468U (en) * | 2018-04-19 | 2018-12-04 | 湖北第二师范学院 | A kind of outdoor plant pathogen pest inoculation protective device |
| CN208279622U (en) * | 2018-01-31 | 2018-12-25 | 山东农业大学 | A kind of living body single blade classification inoculation apparatus |
| CN213153174U (en) * | 2020-09-09 | 2021-05-11 | 连云港市林业技术指导站 | Pesticide injection device for preventing and treating pine wood nematode disease |
| CN213819133U (en) * | 2020-12-10 | 2021-07-30 | 山东农业工程学院 | Pine wood nematode inoculation device |
-
2022
- 2022-05-13 CN CN202210524673.1A patent/CN115250781A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003250337A (en) * | 2002-02-26 | 2003-09-09 | Nara Prefecture | Mycorrhiza organizer and method for forming mycorrhiza |
| CN102726247A (en) * | 2011-04-11 | 2012-10-17 | 乌鲁木齐爱思特专利转让服务有限责任公司 | Seedling raising method |
| US20130224837A1 (en) * | 2012-02-24 | 2013-08-29 | Margret Elaine LEVENTHAL | Inoculation tool and method of use thereof |
| CN103493811A (en) * | 2013-10-21 | 2014-01-08 | 四川农业大学 | Method for prevention and treatment of Bursaphelenchus mucronatus disease |
| CN206933052U (en) * | 2017-06-29 | 2018-01-30 | 北京安和亿泰生物工程技术有限公司 | A kind of nematode Steinernema carpocapsae Propogation and culture ware |
| CN208279622U (en) * | 2018-01-31 | 2018-12-25 | 山东农业大学 | A kind of living body single blade classification inoculation apparatus |
| CN208175468U (en) * | 2018-04-19 | 2018-12-04 | 湖北第二师范学院 | A kind of outdoor plant pathogen pest inoculation protective device |
| CN213153174U (en) * | 2020-09-09 | 2021-05-11 | 连云港市林业技术指导站 | Pesticide injection device for preventing and treating pine wood nematode disease |
| CN213819133U (en) * | 2020-12-10 | 2021-07-30 | 山东农业工程学院 | Pine wood nematode inoculation device |
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