CN208279622U - A kind of living body single blade classification inoculation apparatus - Google Patents
A kind of living body single blade classification inoculation apparatus Download PDFInfo
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- CN208279622U CN208279622U CN201820167835.XU CN201820167835U CN208279622U CN 208279622 U CN208279622 U CN 208279622U CN 201820167835 U CN201820167835 U CN 201820167835U CN 208279622 U CN208279622 U CN 208279622U
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Abstract
The utility model discloses a kind of living body single blade classification inoculation apparatus, including inoculation bag and support rod, lower ending opening, the upper end closed of the inoculation bag, the inoculation bag is fixed on the support rod, the upper end of the inoculation bag is fixed with cotton to moisturizing, and the upper end side wall of the inoculation bag is provided with water filling port, is provided with sealing cover on the water filling port, the upper end of the inoculation bag is provided with inverted U aeration drum capsule pipeline, and the U-shaped gas ducting has closing valve.A kind of living body single blade classification inoculation apparatus provided by the utility model can carry out live leaves Disease Resistance Identification in early stage, can such as identify head blight, powdery mildew, a variety of strains such as stripe rust, moistening effect is good, is conducive to blade and sufficiently falls ill；Living body single blade classification inoculation apparatus in the utility model is with strong points, and structure is simple, is convenient for operation, individually can carry out moisturizing to inoculation blade, integrally grow conducive to plant, reusable, is able to carry out industrialized production.
Description
Technical field
The utility model relates to gramineae plant inoculated identification technical fields, are inoculated with more particularly to a kind of living body single blade
Device.
Background technique
Wheat is important cereal crops, widely plants in the world, supports nearly 35% world population.?
China, wheat are only second to rice, are the second largest staple food grain crop.Therefore, the high yield of wheat and stable yields play national food security
Very important effect.The various biologies and abiotic stress faced during Wheat Production become limitation wheat yield and product
One of an important factor for matter.
Head blight is worldwide widely current caused by Fusarium graminearum (Fusarium graminearum)
Fungal disease.Wheat scab generally occurs in the whole world, is distributed mainly on moist and half tide wet zone, and especially weather is wet
It is aggrieved serious to moisten rainy Temperate Region in China.It can lead to the underproduction, reduces Seed germinating ability and influence grain quality.It catches an illness seed
Grain is due to making them unsuitable for edible and raise livestock containing mycotoxin.In China, head blight tradition diseased region concentrates on growing
River middle and lower reaches Winter Wheat Area and Northeasten Spring Wheat Area of China east, in recent years, the influence of climate and cropping system, diseased region is to the Yellow River and Huai He River wheat
Area, the northern area of wheat and southwest and the extension of the northwest area of wheat.Carrying out breed improvement using scab resistance gene is that mitigation wheat is red
Therefore the fundamental way of mildew harm is also particularly important the identification of head blight.Head blight occurs mainly in wheat fringe
Portion, therefore due to the limitation of growth cycle, we tend not to the resistance for identifying plant pair head blight in advance.And existed by blade
The resistance of early detection plant pair head blight can provide possibility for prevention head blight and relevant scientific research in advance.
Powdery mildew of cereals is that a kind of global gas transmissibility as caused by dlumeria graminis (Blumeria graminis) is true
Fungal disease, main harm wheat, barley, rye, oat etc. seriously affect the quality and yield of wheat crops.The disease occurs
15~20 DEG C of thermophilic, lower than 10 DEG C morbidities are slow.Relative humidity may cause plant disease epidemic when being greater than 70%.
Stripe rust is the fungal disease as caused by bar shaped handle rest fungus (Puccinia striiformis), be wheat, barley,
The Major Diseases of triticale and other grass family plant.Strip rust bacteria belongs to living body parasitism disease fungus, can be with upper air current long distance
From propagation, the environment of low temperature and high relative humidity is conducive to its morbidity.
Currently used inoculation method
1 spraying (trembles powder method)
For the germ invaded from stomata, pay attention to spraying leaf back, leaf back stomata is more positive more.Such as wheat strip rust bacteria
Inoculation, before trembling powder, first humidified using sprayer, with wheat leaf blade surface cover one layer of water mist be advisable, then by talcum powder with
Strip rust bacteria spore mixture (1:5) pours into watering can sprinkling or directly trembles and is sprinkling upon on wheat leaf blade.Due to the presence of water mist, mixture
It is adhered to wheat leaf blade surface.Sprayed back cover plastic film is to keep high humidity environment.
2 semar techniques
Some hosts and spore are all not easy to bedew, is first got wet the blade face that rubs with finger, forms water membrane, then use hair
Spore is applied to above by brush.
3. injection method
The suspension of spore and distilled water is injected into host's growing point or young tender part with injection needle.
4. Vitro Inoculation Technique method
Clip partial blade is placed on solid medium, and then germ is inoculated on blade.
For the above several method primarily directed to the inoculation method of blade, use is more universal, but there is also some defects.
Plant, which falls ill, generally requires the environment of high humidity, but the environment of high humility can inhibit plant growth, because when air humidity is excessive,
Transpiration can weaken, and lead to the ability decline of plant transport mineral nutrition, and plant strain growth reduces, and leaf abscission aggravates, Hua Huo
The vitality of seed reduces.Meanwhile air humidity it is excessive or it is too small can all stomata be caused to be closed, plant stomata close, CO2It cannot
Into mesophyll cell, photosynthesis, which then slows down, even to be stopped.Moreover, high humidity can also make blade face hydrogenesis, blade face cell is caused
Rupture keeps plant weak.
In research of plant disease work, we often need to be inoculated with different materials to identify its disease resistance, still
The method of whole moisturizing will affect plant strain growth, to influence result of study, and the method for Vitro Inoculation Technique can not reflect work
The truth of body plant, therefore, needs for research and at present some defects present in crop leaf inoculation method,
A kind of device that can be individually inoculated with crop living body blade is needed, provides basis for the anti-disease enzyme of plant.
Utility model content
In order to solve the above technical problems, the utility model provides a kind of living body single blade that structure is simple and convenient to operate and connects
Kind device individually can carry out moisturizing to inoculation blade, integrally grow conducive to plant.
To achieve the above object, the utility model provides following technical solution:
The utility model provides a kind of living body single blade classification inoculation apparatus, including inoculation bag and support rod, the inoculation bag
Lower ending opening, upper end closed, the inoculation bag is fixed on the support rod, and the upper end inside the inoculation bag is fixed with guarantor
The upper end side wall of wet cotton, the inoculation bag is provided with water filling port, is provided with sealing cover on the water filling port.
Optionally, the support rod is Multi-section telescopic rod.
Optionally, be provided with inverted U aeration drum capsule pipeline in the inoculation bag, the inverted U aeration drum capsule pipeline with fill
Enraged capsule switch connection.
Optionally, the inoculation bag is polybag, and shape is rectangle, triangle or circle.
Optionally, the inoculation bag is transparent or is protected from light moisturizing bag.
Optionally, the cotton to moisturizing is degreasing cotton fiber.
Optionally, upper end of the cotton to moisturizing plastic packaging inside the inoculation bag.
Optionally, the inoculation bag upper end is fixedly installed partition or partition plastic cement, the cotton to moisturizing be set to it is described every
On plate or partition plastic cement, through-hole is provided on the partition or partition plastic cement.
Optionally, the side of the inoculation bag has a supported hole for accommodating the support rod, the supported hole is open at one end,
It is closed at one end.
Optionally, the inoculation bag is valve bag or the inoculation bag lower end setting that lower ending opening position has self-sealed article
There is sealing strip.
The utility model achieves following technical effect compared with the existing technology:
A kind of living body single blade classification inoculation apparatus provided by the utility model can carry out live leaves disease resistance mirror in early stage
It is fixed, it can such as identify head blight, powdery mildew, a variety of strains such as stripe rust, moistening effect is good, is conducive to blade and sufficiently falls ill；This reality
With strong points with the living body single blade classification inoculation apparatus in novel, structure is simple, is convenient for operation, can individually protect to inoculation blade
It is wet, it is integrally grown conducive to plant, it is reusable, it is able to carry out industrialized production.
Detailed description of the invention
In order to illustrate the embodiment of the utility model or the technical proposal in the existing technology more clearly, below will be to embodiment
Needed in attached drawing be briefly described, it should be apparent that, the accompanying drawings in the following description is only the utility model
Some embodiments for those of ordinary skill in the art without creative efforts, can also be according to this
A little attached drawings obtain other attached drawings.
Fig. 1 is the structural schematic diagram of the living body single blade classification inoculation apparatus in the utility model；
Fig. 2 is the structural schematic diagram that bag is inoculated in the utility model；
Fig. 3 is the structural schematic diagram of Multi-section telescopic rod in the utility model；
Fig. 4 is another structural schematic diagram of living body single blade classification inoculation apparatus in the utility model；
Description of symbols: 1, self-sealed article；2, support rod；3, it is inoculated with bag；4, cotton to moisturizing；5, supported hole；6, sealing strip；7,
Aeration drum capsule switch；8, inverted U aeration drum capsule pipeline；9, partition；10, water filling port.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
To keep the above objects, features, and advantages of the utility model more obvious and easy to understand, with reference to the accompanying drawing and have
Body embodiment is described in further detail the utility model.
As shown in Figure 1, the utility model provides a kind of living body single blade classification inoculation apparatus, including inoculation bag 3 and support rod 2,
It is inoculated with lower ending opening, the upper end closed of bag 3, living body single blade is inserted in inoculation bag 3 by inoculation bag 3 by the lower end being open, so
The lower end closed that inoculation bag 3 is open afterwards, inoculation bag 3 are fixed on support rod 2, are inoculated with the upper end inside bag 3 and are fixed with moisturizing
Cotton 4, the setting of cotton to moisturizing 4 ensure that living body single blade inoculation after growing environment, make inoculation after living body single blade morbidity or
The effect that do not fall ill becomes apparent from, and the upper end of inoculation bag 3 is provided with water filling port 10, and water filling port 10 is used to fill the water cotton to moisturizing 4, water filling
It is provided with sealing cover on mouth 10, in this present embodiment, as shown in figure 4, water filling port 10 is chosen as being set to the upper end side of inoculation bag 3
Wall.
In order to guarantee that cotton to moisturizing 4 is fixed on the upper end being inoculated with inside bag 3, in this present embodiment, as shown in Fig. 1, cotton to moisturizing
4 are chosen as upper end of the plastic packaging inside inoculation bag 3.In another embodiment, as shown in Fig. 4, the fixed setting of inoculation 3 upper end of bag
There is partition 9, cotton to moisturizing 4 is set on partition 9, through-hole is provided on partition 9, further, in order to guarantee that single blade is connecing
It is not contacted directly with inoculation bag 3 in kind bag 3, to influence the living environment of single blade, is inoculated in bag 3 and is provided with inverted U inflation
Drum capsule pipeline 8, inverted U aeration drum capsule pipeline 8 connect with aeration drum capsule switch 7, are chosen as inverted U aeration drum capsule pipeline 8 and connect
The top of kind bag 3 is fixedly connected.In another embodiment, inoculation 3 upper end of bag is fixedly installed partition plastic cement, and cotton to moisturizing 4 is arranged
In on the partition plastic cement, through-hole is provided on the partition plastic cement.
In order to adapt to the difference of different plant heights to be seeded, support rod 2 can be designed as different height, in the present embodiment
In, as shown in figure 3, support rod 2 is selected as Multi-section telescopic rod.
According to different requirement of experiment, the inoculation bag 3 of unlike material can be designed, as papery, plastic material, ventilative material,
Closed material etc., in order to which material selection is convenient, in this present embodiment, inoculation bag 3 is chosen as polybag, and inoculation bag 3 is also chosen as
It is transparent or be protected from light moisturizing bag.
According to different crops blade profiles to be seeded and leaf blade size, in this present embodiment, inoculation bag 3 may be designed as difference
The concrete shape size equal-specification of specification, such as circle, rectangle, triangle, abnormity, inoculation bag 3 is selected according to actual needs.
In order to guarantee the moisture retention of cotton to moisturizing 4, in this present embodiment, as shown in Figs 1-4, cotton to moisturizing 4 is preferably water suction
Property strong degreasing cotton fiber or other can water-absorption fiber, cotton to moisturizing 4 is fixed on the upper end of inoculation bag 3.
According to different tests demand, in this present embodiment, injection water or other liquid reagents may be selected in water filling port 10, with
Realize the specially treated demand to blade.
The fixed form that inoculation bag 3 is fixed on support rod 2 is varied, such as by clamp, in the present embodiment
In, as shown in Figs. 1-2, it will be inoculated with bag 3 for convenience and be fixed on support rod 2, the side of inoculation bag 3, which has, accommodates support rod 2
Supported hole 5, support rod 2 is put into supported hole 5 and the fixed inoculation bag 3 that can be realized is fixed on support rod 2.Further
Ground, in order to be more convenient the support of 2 pairs of support rod inoculation bags 3, supported hole 5 is open at one end, closed at one end.
After blade to be seeded is put into inoculation bag 3, needs the open end to inoculation bag 3 to close, treated with realizing
It is inoculated with the effect of the abundant moisturizing of blade, closed mode is varied, such as not tight by paper clip, in order to guarantee sealing effect
More preferably, in this present embodiment, as shown in Figs. 1-2, inoculation bag 3 is the valve bag that lower ending opening position has self-sealed article 1, when waiting
After kind blade is put into inoculation bag 3, the closing of bag 3 directly will be inoculated with by self-sealed article 1.In another embodiment, such as Fig. 4 institute
Show, inoculation 3 lower end of bag is provided with sealing strip 6, and lower part is folded outward sealing, and the attached adhesive bead in surface, it is broken that surface outfit glassine paper keeps away envelope
Band.
It is two kinds of experiment case studies using the living body single blade classification inoculation apparatus in the utility model below:
1 Fusarium graminearum of experiment case study is inoculated with barley variety Goldenpromise:
' Golden Promise ' is spring habit two rowed barley, can infect head blight.We with syringe pressure by being injected
Method by Fusarium graminearum spore liquid (spore concentration 107A/mL) it is injected into blade from vacuum side of blade, while to another
Blade injector water protects the blade after injection using the single blade classification inoculation apparatus in the utility model as experiment contrast
It is wet, blade incidence is observed after moisturizing three days.It can't see obvious scab behind blade moisturizing three days of injection water, and inject cereal
Obvious scab can be observed in the blade of sickle-like bacteria.Experiment shows that the blade of inoculation does not have the resistance to head blight, this is practical new
Living body single blade classification inoculation apparatus in type can be applied in the inoculated identification of wheat single blade head blight.
2 powdery mildew of experiment case study is inoculated with barley Goldenpromise:
Big wheat powdery mildew (Blumeria graminis f.sp.hordei) is trembled to be inoculated with by the method for trembling powder
On blade, while another blade injector water will be inoculated on the blade of moisturizing bag set after inoculation, adjustment branch as experiment contrast
Strut height, fixed inoculation bag, seals the sack of inoculation bag open end, keeps 100% humidity in inoculation bag, plant is placed on
In the environment of suitable temperature, after inoculation five days, inoculation bag is removed, incidence is observed.The results show that the leaf of inoculation powdery mildew
Piece grows apparent white powder spore after inoculation five days on blade, and is inoculated with the blade of water, does not occur obvious scab.Test table
Bright, the blade of inoculation does not have the resistance to big wheat powdery mildew, and the living body single blade classification inoculation apparatus in the utility model can be applied
In the identification of powdery mildew.
It applies specific case in this specification to be expounded the principles of the present invention and embodiment, the above reality
The explanation for applying example is merely used to help understand the method and its core concept of the utility model；Meanwhile for the general of this field
Technical staff, based on the idea of the present invention, there will be changes in the specific implementation manner and application range.To sum up institute
It states, the content of the present specification should not be construed as a limitation of the present invention.
Claims (10)
1. a kind of living body single blade classification inoculation apparatus, which is characterized in that including inoculation bag and support rod, the lower end of the inoculation bag is opened
Mouth, upper end closed, the inoculation bag are fixed on the support rod, and the upper end inside the inoculation bag is fixed with cotton to moisturizing, institute
The upper end side wall for stating inoculation bag is provided with water filling port, is provided with sealing cover on the water filling port.
2. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that the support rod is multi-joint telescoping
Bar.
3. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that be provided with down U in the inoculation bag
Type aeration drum capsule pipeline, the inverted U aeration drum capsule pipeline and aeration drum capsule switch connect.
4. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that the inoculation bag is polybag, shape
Shape is rectangle, triangle or circle.
5. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that the inoculation bag is transparent or is protected from light
Moisturizing bag.
6. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that the cotton to moisturizing is that absorbent cotton is fine
Dimension.
7. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that the cotton to moisturizing plastic packaging connects in described
Upper end inside kind bag.
8. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that the inoculation bag upper end fixed setting
There are partition or partition plastic cement, the cotton to moisturizing is set on the partition or partition plastic cement, is set on the partition or partition plastic cement
It is equipped with through-hole.
9. living body single blade classification inoculation apparatus according to claim 1, which is characterized in that the side of the inoculation bag, which has, to hold
Receive the supported hole of the support rod, the supported hole is open at one end, closed at one end.
10. living body single blade classification inoculation apparatus described according to claim 1 or 3 or 4, which is characterized in that under the inoculation bag is
There is the valve bag of self-sealed article or the inoculation bag lower end to be provided with sealing strip at end opening position.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820167835.XU CN208279622U (en) | 2018-01-31 | 2018-01-31 | A kind of living body single blade classification inoculation apparatus |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201820167835.XU CN208279622U (en) | 2018-01-31 | 2018-01-31 | A kind of living body single blade classification inoculation apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112080434A (en) * | 2020-08-28 | 2020-12-15 | 中国农业科学院烟草研究所 | Artificial inoculation identification method for alternaria alternate |
| CN115250781A (en) * | 2022-05-13 | 2022-11-01 | 南京林业大学 | Device and method for manually and rapidly inoculating pine wood nematodes |
-
2018
- 2018-01-31 CN CN201820167835.XU patent/CN208279622U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112080434A (en) * | 2020-08-28 | 2020-12-15 | 中国农业科学院烟草研究所 | Artificial inoculation identification method for alternaria alternate |
| CN112080434B (en) * | 2020-08-28 | 2022-05-10 | 中国农业科学院烟草研究所 | Artificial inoculation identification method for alternaria alternata |
| CN115250781A (en) * | 2022-05-13 | 2022-11-01 | 南京林业大学 | Device and method for manually and rapidly inoculating pine wood nematodes |
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