CN106212142A - The in-vitro verification method of bacterial blight of rice - Google Patents
The in-vitro verification method of bacterial blight of rice Download PDFInfo
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- CN106212142A CN106212142A CN201610853418.6A CN201610853418A CN106212142A CN 106212142 A CN106212142 A CN 106212142A CN 201610853418 A CN201610853418 A CN 201610853418A CN 106212142 A CN106212142 A CN 106212142A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The present invention relates to the in-vitro verification method of a kind of bacterial blight of rice, belong to the authentication method technical field of bacterial blight of rice.The in-vitro verification method of this bacterial blight of rice comprises the steps: step one, the preparation of rice material to be identified；Step 2, the activation of rice leaf spot bacteria and cultivation；Step 3, the preparation of excised leaf nutritional solution and preparation；Step 4, the collection of rice varieties excised leaf to be identified；Step 5, prepared by the inoculation bacterium solution of rice leaf spot bacteria；Step 6, the in vitro leaf-cutting method inoculation of rice leaf；Step 7, postvaccinal excised leaf moisturizing is cultivated；Step 8, the investigation of the state of an illness and record.The inventive method uses in indoor in vitro Inoculated Rice bacterial leaf-blight, it is ensured that the concordance of the conditions such as temperature that morbidity needs, humidity, fully ensures susceptible variety morbidity, it is ensured that qualification result true, reliable.
Description
Technical field
The invention belongs to the technical field of the authentication method of bacterial blight of rice, be specifically related to a kind of bacterial blight of rice
In-vitro verification method.
Background technology
Oryza sativa L. is one of most important cereal crops in the whole world, and grain security is had by the guarantee of the stable and quality of its yield
Have and important meaning.Bacterial blight of rice is one of three big diseases of Oryza sativa L., and the safety in production to Oryza sativa L. causes bigger
Threatening, the new rice variety therefore cultivating bacterial blight-resisting is the important goal of modern rice breeding.And to breeding intermediate materials
Carrying out Disease Resistance Identification is the Main Means cultivating disease-resistant variety, at present to the Resistance Identification of bacterial blight of rice still in land for growing field crops
After utilizing shears to dip rice leaf spot bacteria suspension, cut the Oryza sativa L. lobus cardiacus blade of 2-3cM, after causing wound, be easy to Bai Ye
The intrusion of rot bacterium, observes incidence, judges whether kind has with the degree of expansion of scab for after general leaf-cutting 15-20 days
Resistance, the final target realizing screening disease-resistant variety.The Resistance Identification method of bacterial blight of rice is only in field survival at present
Carry out on rice plant, there is no the relevant report of in vitro inoculation.
Any plant disease is required for issuing in certain environmental condition (such as crucial temperature, humidity etc.)
Raw, the land for growing field crops inoculation method of existing bacterial blight of rice is carried out under field conditions (factors), it is impossible to the environment such as the epidemic disaster in control land for growing field crops
Condition, it is impossible to ensure that onset condition is the most suitable, the most just cannot ensure the repeatability that Resistance Identification is tested, often identify disease-resistant
Kind the most disease-resistant, need by inoculated identification repeatedly, waste time and energy, affect breeding process.And field leaf-cutting
Once can only inoculate a piece of leaf, inefficient operation during inoculation, in field, operation element is the most arduous.
Additionally, rice breeding base is often positioned in agricultural production land for growing field crops, i.e. adjoining the Oryza sativa L. of peasant's field planting, carrying out
When field resistance identifies inoculation, bacterium solution can be made to remain on the blade of inoculation, easily propagate to produce land for growing field crops along with wind and rain,
Causing artificial inoculation to endanger, cause damage Production of Large Fields, such as sustained wind rainy day gas, harm can not be despised.
Summary of the invention
The invention aims to solve the deficiencies in the prior art, and the Detached-leaf test of a kind of bacterial blight of rice is provided
Method, the method can fully ensure that susceptible variety is fallen ill, it is ensured that the concordance of onset condition.
The present invention adopts the following technical scheme that
The in-vitro verification method of bacterial blight of rice, comprises the steps:
Step one, the preparation of rice material to be identified: be seeded in seedling dish after rice paddy seed presoaking and germinating to be measured, treat that rice shoot is long
Transplant in land for growing field crops during to 4-5 sheet leaf, carry out field liquid manure and the pest management of routine, treat that paddy growth is to tillering regularity；
Step 2, the activation of rice leaf spot bacteria and cultivation: take out the rice leaf spot bacteria preserved, root from ultra cold storage freezer
(bacterial blight of rice former bacterium serological Identification divides three serotypes, divides to need to select different types of spawn according to Disease Resistance Identification
Be not I type be advantage type, the distribution whole nation, II, III type exists only in indivedual rice district, south.Pathogen growth temperature limits 17-33 DEG C, the suitableeest
25-30 DEG C, minimum 5 DEG C, the highest 40 DEG C, pathogenic bacteria optimum pH6.5-7.0), its training method is consistent with required culture environment,
I.e. directly on aseptic operating platform, dip appropriate bacterium solution activation culture in NA culture medium standby after one week；
Step 3, the preparation of excised leaf nutritional solution and preparation: will prepare and cultivate battalion through autoclaved Detached Rice Leaves
Nutrient solution pours containers for future use into, and wherein the composition of nutritional solution is: sucrose 3%, citric acid 150mg/L, aluminum sulfate 200mg/L, 8-hydroxyl
Base quinoline 40mg/L, nutrient composition can be referring in detail to document: Li Haiqing, Zhang Ling, Yuan Guotao, Xie Chonghua. and rice leaf is in vitro
Preservation technique is explored, hubei agricultural science, and 2011,50(3): 468-471；
Step 4, the collection of rice varieties excised leaf to be identified: each kind (stablizes product from the rice plant of tillering regularity
System) or individual plant (segregating population) take three consistent fully deployed blades of length, write the numbering of kind with permanent pen
After, it is bundled into a bundle after being put in order one end of Herba Lysimachiae Insignis sheet neatly, then neat one end is put in large beaker down, as same in needed
Time detect multiple kind and then repeat aforesaid operations；
Step 5, prepared by the inoculation bacterium solution of rice leaf spot bacteria: by cultured rice leaf spot bacteria bacterium colony applicator
Dilute with sterilized water after culture dish eluting, standby；
Step 6, the in vitro leaf-cutting method inoculation of rice leaf:
Take the shears of a cleaning, blade is completely submerged in the bacterium solution of step 5 and takes out rapidly, along step 4 Leaf
After the blade of 2-3cM is cut in most advanced and sophisticated cross section, squirt the part not being immersed in nutritional solution with sterilized water；
Step 7, postvaccinal excised leaf moisturizing is cultivated: overlaps a transparent freshness protection package at beaker opening, then ties bag
Mouthful, beaker is cultivated 10-15 days as continuous moisturizing under photoenvironment；
Step 8, the investigation of the state of an illness and record: after susceptible check variety morbidity fully, institute an inquiry the state of an illness, by measuring leaf
Sheet scab length, according to investigation standard survey scab length and resistance rank.
Further, the in-vitro verification method of described bacterial blight of rice, water wherein to be measured described in step one
Rice comprises susceptible comparison known to and disease-resistant comparison, and the Transplanting Density of rice shoot is 22cm*22cm.
Further, the in-vitro verification method of described bacterial blight of rice, wherein ultralow temperature ice described in step 2
The temperature of case is-80 DEG C, and described rice leaf spot bacteria is stored in glycerol.
Further, the in-vitro verification method of described bacterial blight of rice, wherein by after described eluting in step 4
Rice leaf spot bacteria bacterium colony sterilized water to be diluted to concentration be OD600=0.5.
Further, the in-vitro verification method of described bacterial blight of rice, wherein soaks blade in step 6 completely
Do not take out after 2s in the bacterium solution of step 5.
Further, the in-vitro verification method of described bacterial blight of rice, wherein beaker described in step 7 is 2L
Plastic beaker, by beaker as under the photoenvironment of 28 DEG C continuous moisturizing cultivate 10-15 days.
Bacterial blight of rice causes serious economic loss to the safety in production of Oryza sativa L., and it is the most pre-for cultivating disease-resistant variety
The method that anti-bacterial blight of rice occurs.And it is the necessary links cultivating disease-resistant variety that breeding material carries out Disease Resistance Identification
One of, the present invention intends being formed indoor excised leaf leaf-cutting inoculation Disease Resistance Identification in a kind of rice bacterial blight resistance breeding process
Method, thus it is greatly improved success rate and the effect of Disease Resistance Identification,
Compared with prior art, beneficial effects of the present invention:
First: this method uses in indoor in vitro Inoculated Rice bacterial leaf-blight, it is ensured that temperature that morbidity needs, wet
The conditions such as degree, fully ensure susceptible variety morbidity, it is ensured that the concordance of onset condition；
Second: this method uses indoor in vitro inoculation can once carry out the inoculation of more blades, and operation is convenient, and efficiency is high, adjusts
When looking into the most time saving and energy saving；
3rd: this method uses indoor in vitro inoculation to be possible to prevent the pathogenic bacteria caused when inoculating in land for growing field crops artificially to spread, thus anti-
Only field rice is produced the potential hazard caused；
4th: the indoor inoculation method that the present invention provides, than traditional short 5-10d of Field inoculation disease time, avoids simultaneously and connects
The personnel of kind, in the arduous work in field, improve work efficiency.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and should not be regarded as limiting this
Bright scope.
Embodiment
Step one: the preparation of rice material to be identified
After rice paddy seed (comprising susceptible comparison known to and disease-resistant comparison) presoaking and germinating to be measured, it is seeded in seedling dish, treats
Transplant in land for growing field crops when rice shoot length is to 4-5 sheet leaf, Transplanting Density 22cm*22cm, carry out field liquid manure and the pest management of routine,
Treat that paddy growth is to tillering regularity (about the general transplanting later moon)；
Step 2: the activation of rice leaf spot bacteria and cultivation
The rice leaf spot bacteria being stored in glycerol taken out from-80 DEG C of ultra cold storage freezers, can be according to Disease Resistance Identification needs
Select different types of spawn (bacterial blight of rice former bacterium serological Identification divides three serotypes, be respectively I type be advantage type,
The distribution whole nation, II, III type exists only in indivedual rice district, south.Pathogen growth temperature limits 17-33 DEG C, the suitableeest 25-30 DEG C, minimum 5 DEG C,
The highest 40 DEG C, pathogenic bacteria optimum pH6.5-7.0), its training method is consistent with required culture environment.I.e. directly in sterile working
Appropriate bacterium solution activation culture in NA culture medium (Nutrient Agar) is dipped standby after one week on platform；
Step 3: the preparation of excised leaf nutritional solution and preparation
Appropriate (about 200mL) is prepared and pour the plastics large beaker of 2L through autoclaved Detached Rice Leaves cultivation nutritional solution into
In standby；
Step 4: the collection of rice varieties excised leaf to be identified
From the rice plant of tillering regularity, each kind (stablizing strain) or individual plant (segregating population) take the consistent (length of three length
About 20cM) fully deployed blade, write the numbering of kind with permanent pen after, with a rubber band by the one of Herba Lysimachiae Insignis sheet
End is bundled into a bundle with rubber band after putting in order neatly, is then put into down in large beaker neat one end, as many in need to be detected simultaneously
Individual kind then repeats aforesaid operations；
Step 5: prepared by the inoculation bacterium solution of rice leaf spot bacteria
Cultured rice leaf spot bacteria bacterium colony applicator is diluted with sterilized water after culture dish eluting, is finally diluted to
Concentration is standby after OD600=0.5；
Step 6: the in vitro leaf-cutting method inoculation of rice leaf
Take the shears of a cleaning, blade is totally submerged in the bacterium solution in step 5 and takes out after 2 seconds, along step 4 Leaf point
Cross section is held to squirt, after cutting the blade of 2-3cM, the part not being immersed in nutritional solution with sterilized water；
Step 7: postvaccinal excised leaf moisturizing is cultivated
Plastic beaker opening at 2L overlaps a transparent freshness protection package, then ties bag mouth with rubber band, by beaker as 28
DEG C illumination photoenvironment under continuously moisturizing cultivate 10-15 days；
Step 8: the investigation of the state of an illness and record
The state of an illness is instituted an inquiry, by measuring leaf spot lesion length, according to the tune of Gu etc. after susceptible check variety morbidity fully
Look into standard survey scab length and resistance rank, its anti-sense criterion following (criterion refers to list of references: Gu k,
Tian D, Yang F, et al. High-resolution genetic mapping of Xa27(t),a new
bacterial blight resistance gene in rice. Oryza sativa L. Theor Appl Genet,
2004,108:800 807):
R(Resistance, disease-resistant): scab length 3.0cm
MR(Middle Resistance, in anti-): 3.0cm scab length 6.0cm
MS(Middle Sensitivity, middle sense): 6.0 cm scab length 9.0cm
S(Sensitivity, susceptible): scab length 9.0cm.
Utilize the inventive method identify bacterial blight of rice test examples:
Rice material: (1) bacterial leaf spot resistance donor material CBB23 is provided by Institute of Crop Science, Chinese Academy of Agricultural Science；
(2) acceptor material is bright extensive 86, by Rice Research Institute, Fujian Academy of Agricultural Science's selection-breeding；
Qualification fungus strain: Zhejiang 173(IV type) it is south China rice district and the Prominent pathogen in rice district, the Yangtze river basin, by Chinese agriculture section
Institute's crop science institute provides.
This test, with bright extensive as receptor parent, hybridize for donor parents with CBB23, and returns for recurrent parent with bright extensive 86
Hand over, plant segregating population, by this method, the individual plant separated is carried out the Resistance Identification of bacterial blight of rice.In combination with molecule mark
Note assisted Selection is mutually authenticated qualification result, selects with target gene in field and shows as disease-resistant individual plant as breeding
Target, the partial results of this test Resistance Identification such as table 1 below:
Table 1 Resistance Identification result
Skilled person will appreciate that of the industry, the present invention is not restricted to the described embodiments, in above-described embodiment and description
The principle that the present invention is simply described described, without departing from the spirit and scope of the present invention, the present invention also has various
Changes and improvements, these changes and improvements both fall within scope of the claimed invention.Claimed scope is by institute
Attached claims and equivalent thereof define.
Claims (6)
1. the in-vitro verification method of bacterial blight of rice, it is characterised in that comprise the steps:
Step one, the preparation of rice material to be identified: be seeded in seedling dish after rice paddy seed presoaking and germinating to be identified, treat rice shoot
Transplant in land for growing field crops when length is to 4-5 sheet leaf, carry out field liquid manure and the pest management of routine, treat that paddy growth is to tillering regularity；
Step 2, the activation of rice leaf spot bacteria and cultivation: take out the rice leaf spot bacteria preserved, root from ultra cold storage freezer
Needing to select different types of spawn according to Disease Resistance Identification, its training method is consistent with required culture environment, i.e. directly in nothing
Appropriate bacterium solution activation culture in NA culture medium is dipped standby after one week on bacterium operating board；
Step 3, the preparation of excised leaf nutritional solution and preparation: will prepare and through autoclaved Detached Rice Leaves
Cultivate nutritional solution and pour containers for future use into；
Step 4, the collection of rice varieties excised leaf to be identified: each kind or individual plant from the rice plant of tillering regularity
Take three consistent fully deployed blades of length, write the numbering of kind with permanent pen, one end of Herba Lysimachiae Insignis sheet is returned
It is bundled into a bundle after putting neatly, then neat one end is put in large beaker down, then repeat as need to be detected multiple kind simultaneously
Aforesaid operations；
Step 5, prepared by the inoculation bacterium solution of rice leaf spot bacteria: by cultured rice leaf spot bacteria bacterium colony applicator
Dilute with sterilized water after culture dish eluting, standby；
Step 6, the in vitro leaf-cutting method inoculation of rice leaf:
Take the shears of a cleaning, blade is completely submerged in the bacterium solution of step 5 and takes out rapidly, along step 4 Leaf
After the blade of 2-3cM is cut in most advanced and sophisticated cross section, squirt the part not being immersed in nutritional solution with sterilized water；
Step 7, postvaccinal excised leaf moisturizing is cultivated: overlaps a transparent freshness protection package at beaker opening, then ties bag
Mouthful, form an environment closed, beaker is cultivated 10-15 days as continuous moisturizing under photoenvironment；
Step 8, the investigation of the state of an illness and record: after susceptible check variety morbidity fully, institute an inquiry the state of an illness, by measuring leaf
Sheet scab length, according to investigation standard survey scab length and resistance rank.
The in-vitro verification method of bacterial blight of rice the most according to claim 1, it is characterised in that treat described in step one
The rice paddy seed surveyed comprises susceptible comparison known to and disease-resistant comparison, and the Transplanting Density of rice shoot is 22cm*22cm.
The in-vitro verification method of bacterial blight of rice the most according to claim 1, it is characterised in that super described in step 2
The temperature of cryogenic refrigerator is-80 DEG C, and described rice leaf spot bacteria is stored in glycerol.
The in-vitro verification method of bacterial blight of rice the most according to claim 1, it is characterised in that by described in step 4
It is OD600=0.5 that rice leaf spot bacteria bacterium colony sterilized water after eluting is diluted to concentration.
The in-vitro verification method of bacterial blight of rice the most according to claim 1, it is characterised in that by blade in step 6
It is completely submerged in the bacterium solution of step 5 and takes out after 2s.
6. according to the in-vitro verification method of the bacterial blight of rice described in any one of claim 1 to 5, it is characterised in that step
Beaker described in seven is the plastic beaker of 2L, beaker is placed under the photoenvironment of 28 DEG C continuous moisturizing and cultivates 10-15 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486033A (en) * | 2018-02-23 | 2018-09-04 | 云南省德宏热带农业科学研究所 | A kind of in-vitro verification method of coffee germplasm rust resistance |
| CN116602205A (en) * | 2023-05-25 | 2023-08-18 | 福建省农业科学院水稻研究所 | Breeding method of new disease-resistant rice material |
| CN117571935A (en) * | 2024-01-15 | 2024-02-20 | 海南大学三亚南繁研究院 | Application of TDZ solution in-vitro identification of bacterial leaf blight resistance of rice and identification method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1695434A (en) * | 2005-06-09 | 2005-11-16 | 南京农业大学 | Method for partitioning germ microspecies of bacterial leaf spot of paddy rice |
| CN102487742A (en) * | 2011-12-05 | 2012-06-13 | 江西省农业科学院植物保护研究所 | Method for identifying rice blast resistance of rice |
| CN103205501A (en) * | 2013-04-19 | 2013-07-17 | 云南省农业科学院农业环境资源研究所 | Method for identifying rice blast-resistant gene of wild rice |
| CN104521744A (en) * | 2015-01-04 | 2015-04-22 | 黎建波 | New bacterial-blight-resisting japonica rice breeding method |
-
2016
- 2016-09-27 CN CN201610853418.6A patent/CN106212142A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1695434A (en) * | 2005-06-09 | 2005-11-16 | 南京农业大学 | Method for partitioning germ microspecies of bacterial leaf spot of paddy rice |
| CN102487742A (en) * | 2011-12-05 | 2012-06-13 | 江西省农业科学院植物保护研究所 | Method for identifying rice blast resistance of rice |
| CN103205501A (en) * | 2013-04-19 | 2013-07-17 | 云南省农业科学院农业环境资源研究所 | Method for identifying rice blast-resistant gene of wild rice |
| CN104521744A (en) * | 2015-01-04 | 2015-04-22 | 黎建波 | New bacterial-blight-resisting japonica rice breeding method |
Non-Patent Citations (2)
| Title |
|---|
| 湖南长沙农业学校: "《农作物病虫害防治学 南方本》", 29 February 1980, 农业出版社 * |
| 章正: "《植物种传病害与免疫》", 28 February 2011, 中国农业出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108486033A (en) * | 2018-02-23 | 2018-09-04 | 云南省德宏热带农业科学研究所 | A kind of in-vitro verification method of coffee germplasm rust resistance |
| CN116602205A (en) * | 2023-05-25 | 2023-08-18 | 福建省农业科学院水稻研究所 | Breeding method of new disease-resistant rice material |
| CN117571935A (en) * | 2024-01-15 | 2024-02-20 | 海南大学三亚南繁研究院 | Application of TDZ solution in-vitro identification of bacterial leaf blight resistance of rice and identification method |
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