CN114948789B - SOD composition with blue light resisting effect and preparation method thereof - Google Patents
SOD composition with blue light resisting effect and preparation method thereof Download PDFInfo
- Publication number
- CN114948789B CN114948789B CN202210503117.6A CN202210503117A CN114948789B CN 114948789 B CN114948789 B CN 114948789B CN 202210503117 A CN202210503117 A CN 202210503117A CN 114948789 B CN114948789 B CN 114948789B
- Authority
- CN
- China
- Prior art keywords
- blue light
- sod
- light resistance
- mass
- purified water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/738—Cyclodextrins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/49—Solubiliser, Solubilising system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Emergency Medicine (AREA)
- Dermatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to an SOD composition with efficient blue light resistance and a preparation method thereof. The SOD composition with the efficient blue light resistance provided by the invention takes SOD as a core raw material, is matched with other preferred polypeptides, stabilizers and synergists, solves the problems of poor stability, low absorption and utilization rate and the like of the SOD in application through compatibility of specific proportions, can greatly improve the effect of the SOD on blue light resistance on repairing damage, and is an ideal cosmetic composition for resisting blue light and repairing skin damage.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an SOD composition with a blue light resisting effect and a preparation method thereof.
Background
The research shows that all-band sunlight can damage human skin, including infrared rays, visible light and ultraviolet rays. Blue light is an important component of visible light, and LED lamps and electronic screens in daily life are also very common. Blue light is the highest energy band in visible light, and has the strongest penetrating power, even stronger than ultraviolet light, so that blue light can cause skin damage similar to that caused by ultraviolet light.
The blue light irradiates the keratinocyte to enable the cell to generate oxidative stress, the cell receives the blue light to generate super-oxygen anions mainly, the cell receives the UVA ultraviolet light with the wavelength of 320-420 nm to generate singlet oxygen mainly, both the ultraviolet light and the ultraviolet light mediate skin injury through a Reactive Oxygen Species (ROS) way, the cell is induced to generate free radicals, the antioxidation capability of the fibroblast is reduced, the proliferation of the fibroblast is inhibited, the extracellular matrix of the dermis of the skin is greatly reduced, and the aging speed of the skin is further accelerated.
Currently, blue light resistant cosmetics on the market generally add ingredients such as plant extracts or plant essential oils to block damage of blue light to skin. For example: patent document CN 106727027a discloses a composition for resisting blue light, resisting oxidation and scavenging free radicals. The composition for resisting blue light, resisting oxidation and scavenging free radicals mainly comprises a marigold flower extract and safflower seed oil compound, a tea extract and a alpine leontopodium herb extract, wherein the marigold flower extract and safflower seed oil compound can enhance the blue light filtering capability of human skin, prevent the damage of the blue light to the skin, capture free radicals in human skin tissues and delay aging caused by skin oxidation.
Patent document CN 109464308A discloses an essential oil composition with blue light resisting and skin protecting effects, which mainly comprises orange flower essential oil, pomegranate essential oil, ginger essential oil and lavender essential oil, and the prepared essential oil composition can absorb blue light so as to avoid damage of the blue light to skin, and simultaneously has the effects of promoting blood circulation, whitening and removing freckle, promoting cell growth, activating blood and dissolving stasis, resisting free radicals, and can eliminate skin injury and skin aging caused by the blue light, thereby achieving the purpose of resisting the blue light.
However, the blue light resistant cosmetic added with the plant extract or the plant essential oil has the defects of poor blue light resistant effect, short repairing time, easy volatilization of the plant essential oil and quick decrease of the blue light resistant effect in the using process. Superoxide dismutase (SOD) is a metalloprotease containing copper, zinc, iron and manganese, has the functions of catalyzing superoxide anion free radicals to perform disproportionation reaction and balancing oxygen free radicals in a body, and is expected to become an important component for resisting skin aging caused by blue light radiation.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides an SOD composition with blue light resistance and a preparation method thereof. The SOD composition with the blue light resistance provided by the invention takes SOD as a core raw material, is matched with other preferred polypeptides, stabilizers and synergists, solves the problems of poor stability, low utilization rate and the like of the SOD in application through compatibility of specific proportions, and can also greatly improve the blue light resistance and damage repair effects of the SOD.
The invention provides an SOD composition with blue light resistance, which comprises the following components in percentage by mass:
0.01 to 2 percent of superoxide dismutase, 0.01 to 2 percent of polypeptide, 5 to 10 percent of stabilizer, 2 to 5 percent of synergistic agent, 10 to 30 percent of solubilizer and 100 percent of purified water.
Further, the SOD composition with the blue light resistance comprises the following components in percentage by mass:
0.1 to 2 percent of superoxide dismutase, 0.1 to 2 percent of polypeptide, 5 to 8 percent of stabilizer, 2 to 5 percent of synergistic agent, 10 to 28 percent of solubilizer and 100 percent of purified water.
Further, the polypeptide is one or a combination of more than two of carnosine, glutathione, acetyl tetrapeptide-2 and palmitoyl tetrapeptide-10.
Further, the stabilizer is one or two of hydroxyethyl piperazine ethane sulfonic acid, trehalose and sodium hyaluronate.
Further, the synergist is p-hydroxyacetophenone.
Further, the solubilizer is one or two of butanediol and pentanediol.
Further, the SOD composition with the blue light resistance effect also comprises 5-10% of cyclodextrin by mass percent.
In addition, the invention also provides a preparation method of the SOD composition with the blue light resistance, which comprises the following steps:
s1, adding a synergistic agent into purified water, heating to 80-100 ℃, and stirring under the condition of 250-350 rpm until the mixture is clear and transparent to obtain a mixed solution;
s2, cooling the mixed solution prepared in the step S1 to normal temperature, then adding superoxide dismutase, polypeptide, stabilizer and solubilizer, and stirring at 450-550 rpm until the mixed solution is clear and transparent, thus obtaining the product.
Further, the preparation method of the SOD composition with the blue light resistance further comprises a step S3, specifically: adding cyclodextrin into purified water to prepare a cyclodextrin saturated solution, adding the mixed solution prepared in the step S2 into the cyclodextrin saturated solution, heating to 60-80 ℃ and stirring for 2-3 h, cooling to room temperature, stirring for 6-8 h at room temperature, standing for 12h at 2-8 ℃, taking out, filtering, taking out crystals, adding purified water and stirring to a clear and transparent state, wherein the feed liquid ratio of the crystals to the purified water is 1 g/6 mL, and obtaining the cyclodextrin.
At present, although SOD has been widely used in skin care products, SOD still has many unsolved problems in the cosmetic industry, such as: SOD has poor stability, and is easy to be influenced by dissolved oxygen, illumination, electrolyte, temperature and other factors to degrade activity in a water-based system; and the molecular weight of the natural SOD is about 32000Da, belongs to macromolecular proteins, has no specific receptor on cell membranes, is difficult to quickly enter cells to play a role, and greatly limits the efficacy of the SOD.
In order to solve the above problems, the present inventors creatively propose an SOD composition having high stability and remarkable anti-blue light effect and skin damage repairing effect. The SOD composition takes SOD as a core raw material, is matched with polypeptide, a stabilizer and a synergistic agent, solves the problems of poor stability and low utilization rate of the SOD by specific proportion compatibility, and can also greatly improve the blue light resistance of the SOD.
During the course of the study, the inventors unexpectedly found that: the stabilizer is formed by mixing the hydroxyethyl piperazine ethane sulfonic acid, one or two of trehalose and sodium hyaluronate, the p-hydroxyacetophenone is used as a synergistic agent, the butanediol and the pentanediol are used as solubilizers, so that the stability of the SOD can be improved, and the polypeptide is further added to be matched with the SOD in a synergistic way, so that the blue light resistance of the SOD can be greatly improved, and the skin injury can be repaired.
The inventors found during the research that although trehalose and sodium hyaluronate are commonly used as moisturizers in cosmetics and indeed more as moisturizing ingredients in terms of human efficacy, the inventors found that raw materials are two different concepts of understanding in terms of the structural effect of the formulation and the effect of the human. The stabilizer formed by mixing the hydroxyethyl piperazine ethane sulfonic acid, the trehalose and the sodium hyaluronate mainly plays a role in maintaining the stability of the pH value of the system and weakening the efficiency of Newton fluid molecules and Brownian movement in the formula system, solves the problem of enzyme activity loss caused by self-digestion reaction of the enzyme activity of SOD in a solution, thereby maintaining the enzyme activity of the SOD in the system and improving the absorption rate of the SOD. The p-hydroxyacetophenone is used as a synergistic agent to have a synergistic effect on the aspect of blue light prevention and antioxidation, so that the blue light prevention and antioxidation of the system are further improved; the butanediol and the pentanediol are used as solubilizers, so that the solubility in the system can be improved, the system is in a stable, clear and transparent state, and the stability of the product is facilitated.
In addition, the inventor also proposes to add cyclodextrin in a certain proportion to seal and wrap the SOD, which can better solve the problem that the SOD is degraded by dissolved oxygen, light, electrolyte, temperature, microorganism and other factors in a water-based system to influence the enzyme activity, further improve the stability of the SOD and is more beneficial to the exertion of the blue light resistance and damage repair effects of the SOD.
The SOD composition with blue light resistance is transparent viscous liquid with colorless to light green appearance. Experiments show that the SOD composition has high activity stability for a long time, has low superoxide dismutase activity damage after high-temperature acceleration test at 40 ℃ in 90 days, and has high-efficiency blue light damage protection effect in the wavelength range of 400-500 nm.
Compared with the prior art, the SOD composition with the blue light resistance has the advantages of high stability, good skin absorptivity, remarkable blue light resistance and damage repair effects, capability of maintaining the blue light resistance and the skin damage repair effects for a long time, simple preparation process, suitability for large-scale production and use, and suitability for ideal blue light resistance and skin damage repair cosmetic composition.
Detailed Description
The invention is further illustrated by the following description of specific embodiments, which are not intended to be limiting, and various modifications or improvements can be made by those skilled in the art in light of the basic idea of the invention, but are within the scope of the invention without departing from the basic idea of the invention. The materials and reagents involved in the present invention are all available commercially or by means conventional in the art. Wherein: SOD and polypeptides were purchased from south beijing bevaceae biotechnology, inc, and cyclodextrin was purchased from dactyl (shanghai) inc.
Example 1 SOD composition having blue light resistance
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 0.2%, polypeptide 0.2%, stabilizer 6%, p-hydroxyacetophenone 5%, solubilizer 10%, purified water added to 100%;
the polypeptide is formed by mixing 0.1% of carnosine by mass and 0.1% of glutathione by mass.
The stabilizer is formed by mixing 5% of hydroxyethyl piperazine ethane sulfonic acid and 1% of sodium hyaluronate.
The solubilizer is formed by mixing 5% of butanediol by mass and 5% of pentanediol by mass.
The preparation process comprises the following steps:
s1, adding p-hydroxyacetophenone into purified water, heating to 80 ℃, and stirring under the condition of 350rpm until the mixture is clear and transparent to obtain a mixed solution;
s2, cooling the mixed solution prepared in the step S1 to normal temperature, then adding superoxide dismutase, polypeptide, stabilizer and solubilizer, and stirring at 550rpm until the mixed solution is clear and transparent.
Example 2SOD composition with blue light resistance
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 0.5%, polypeptide 0.5%, stabilizer 7%, p-hydroxyacetophenone 2%, solubilizer 15%, purified water added to 100%;
the polypeptide is formed by mixing and combining 0.25% of glutathione by mass and 0.25% of acetyl tetrapeptide-2 by mass;
the stabilizer is formed by mixing 5% of hydroxyethyl piperazine ethane sulfonic acid, 1% of trehalose and 1% of sodium hyaluronate;
the solubilizer is formed by mixing and combining 10% by mass of butanediol and 5% by mass of pentanediol.
The preparation process comprises the following steps:
s1, adding p-hydroxyacetophenone into purified water, heating to 90 ℃, and stirring at 300rpm until the mixture is clear and transparent to obtain a mixed solution;
s2, cooling the mixed solution prepared in the step S1 to normal temperature, then adding superoxide dismutase, polypeptide, stabilizer and solubilizer, and stirring at 400rpm until the mixed solution is clear and transparent.
Example 3 SOD composition with blue light resistance
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 1%, polypeptide 1%, stabilizer 10%, p-hydroxyacetophenone 5%, solubilizer 25%, purified water added to 100%;
the polypeptide is formed by mixing 0.25% of carnosine by mass, 0.25% of glutathione by mass and 0.5% of palmitoyl tetrapeptide-10 by mass.
The stabilizer is formed by mixing 5% of hydroxyethyl piperazine ethane sulfonic acid, 4% of trehalose and 1% of sodium hyaluronate.
The solubilizer is formed by mixing and combining 5% by mass of butanediol and 20% by mass of pentanediol.
The preparation process comprises the following steps:
s1, adding p-hydroxyacetophenone into purified water, heating to 100 ℃, and stirring under the condition of 250rpm until the mixture is clear and transparent to obtain a mixed solution;
s2, cooling the mixed solution prepared in the step S1 to normal temperature, then adding superoxide dismutase, polypeptide, stabilizer and solubilizer, and stirring at 450rpm until the mixed solution is clear and transparent.
Comparative example 1, SOD composition having blue light resistance
Superoxide dismutase 0.5%, purified water was added to 100%.
The preparation process comprises the following steps:
and (3) taking superoxide dismutase and purified water, and stirring under the condition of 300rpm until the system is clear and transparent.
Comparative example 2SOD composition having blue light resisting effect
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 1%, stabilizer 7%, p-hydroxyacetophenone 2%, solubilizer 15%, purified water added to 100%;
the stabilizer is formed by mixing 5% of hydroxyethyl piperazine ethane sulfonic acid, 1% of trehalose and 1% of sodium hyaluronate;
the solubilizer is formed by mixing and combining 10% by mass of butanediol and 5% by mass of pentanediol.
The difference from example 2 is that: the polypeptide is absent.
The preparation process was similar to that of example 2.
Comparative example 3 SOD composition having blue light resisting effect
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 0.5%, polypeptide 0.5%, stabilizer 7%, solubilizer 15%, purified water added to 100%;
the polypeptide is formed by mixing and combining 0.25% of glutathione by mass and 0.25% of acetyl tetrapeptide-2 by mass;
the stabilizer is formed by mixing 5% of hydroxyethyl piperazine ethane sulfonic acid, 1% of trehalose and 1% of sodium hyaluronate;
the solubilizer is formed by mixing and combining 10% by mass of butanediol and 5% by mass of pentanediol.
The difference from example 2 is that: p-hydroxyacetophenone is absent.
The preparation process was similar to that of example 2.
Comparative example 4 SOD composition having blue light resisting effect
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
0.5% of superoxide dismutase, 0.5% of polypeptide, 7% of hydroxyethyl piperazine ethane sulfonic acid, 2% of p-hydroxyacetophenone, 15% of solubilizer and adding purified water to 100%;
the polypeptide is formed by mixing and combining 0.25% of glutathione by mass and 0.25% of acetyl tetrapeptide-2 by mass;
the solubilizer is formed by mixing and combining 10% by mass of butanediol and 5% by mass of pentanediol.
The difference from example 2 is that: the stabilizer is hydroxyethyl piperazine ethane sulfonic acid.
The preparation process was similar to that of example 2.
Comparative example 5 SOD composition with blue light resisting effect
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 0.5%, polypeptide 0.5%, stabilizer 7%, p-hydroxyacetophenone 2%, solubilizer 15%, purified water added to 100%;
the polypeptide is formed by mixing and combining 0.25% of glutathione by mass and 0.25% of acetyl tetrapeptide-2 by mass;
the stabilizer is formed by mixing 6% of trehalose by mass and 1% of sodium hyaluronate by mass;
the solubilizer is formed by mixing and combining 10% by mass of butanediol and 5% by mass of pentanediol.
The difference from example 2 is that: the stabilizer is formed by mixing 3.5% of trehalose by mass and 3.5% of sodium hyaluronate by mass.
The preparation process was similar to that of example 2.
Example 4 SOD composition with blue light resistance
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 0.5%, polypeptide 0.5%, stabilizer 7%, p-hydroxyacetophenone 2%, solubilizer 15%, 5% cyclodextrin, purified water added to 100%;
the polypeptide is formed by mixing and combining 0.25% of glutathione by mass and 0.25% of acetyl tetrapeptide-2 by mass;
the stabilizer is formed by mixing 5% of hydroxyethyl piperazine ethane sulfonic acid, 1% of trehalose and 1% of sodium hyaluronate;
the solubilizer is formed by mixing and combining 10% by mass of butanediol and 5% by mass of pentanediol.
The preparation process comprises the following steps:
s1, adding p-hydroxyacetophenone into purified water, heating to 90 ℃, and stirring at 300rpm until the mixture is clear and transparent to obtain a mixed solution;
s2, cooling the mixed solution prepared in the step S1 to normal temperature, then adding superoxide dismutase, polypeptide, stabilizer and solubilizer, and stirring at 400rpm until the mixed solution is clear and transparent;
and S3, adding cyclodextrin into purified water to prepare a cyclodextrin saturated solution, adding the mixed solution prepared in the step S2 into the cyclodextrin saturated solution, heating to 70 ℃, stirring for 2 hours, cooling to room temperature, stirring for 7 hours at room temperature, standing for 12 hours at the temperature of 4 ℃, taking out, filtering, taking out crystals, adding purified water, stirring to a clear and transparent state, and obtaining the product, wherein the feed liquid ratio of the crystals to the purified water is 1 g/6 mL.
Example 5 SOD composition with blue light resistance
The SOD composition with the blue light resistance consists of the following components in percentage by mass:
superoxide dismutase 1%, polypeptide 1%, stabilizer 10%, p-hydroxyacetophenone 8%, solubilizer 25%, 8% cyclodextrin, purified water added to 100%;
the polypeptide is formed by mixing 0.25% of carnosine by mass, 0.25% of glutathione by mass and 0.5% of palmitoyl tetrapeptide-10 by mass.
The stabilizer is formed by mixing 5% of hydroxyethyl piperazine ethane sulfonic acid, 4% of trehalose and 1% of sodium hyaluronate.
The solubilizer is formed by mixing and combining 5% by mass of butanediol and 20% by mass of pentanediol.
The preparation process comprises the following steps:
s1, adding p-hydroxyacetophenone into purified water, heating to 90 ℃, and stirring at 300rpm until the mixture is clear and transparent to obtain a mixed solution;
s2, cooling the mixed solution prepared in the step S1 to normal temperature, then adding superoxide dismutase, polypeptide, stabilizer and solubilizer, and stirring at 400rpm until the mixed solution is clear and transparent;
and S3, adding cyclodextrin into purified water to prepare a cyclodextrin saturated solution, adding the mixed solution prepared in the step S2 into the cyclodextrin saturated solution, heating to 60 ℃, stirring for 2 hours, cooling to room temperature, stirring at room temperature for 8 hours, standing for 12 hours at the temperature of 4 ℃, taking out, filtering, taking out crystals, adding purified water, stirring to a clear and transparent state, and obtaining the product, wherein the feed liquid ratio of the crystals to the purified water is 1 g/6 mL. Test example I stability test of SOD composition with blue light resistance
1. Test object:
SOD compositions having blue light resistance obtained in example 1, example 2, example 3, example 4, example 5, comparative example 1, comparative example 2, comparative example 3, comparative example 4 and comparative example 5.
2. The test method comprises the following steps:
10mL of each of the SOD composition samples having the blue light resistance obtained in example 1, example 2, example 3, example 4, example 5, comparative example 1, comparative example 2, comparative example 3, comparative example 4 and comparative example 5 was placed in a sealed penicillin bottle, and the samples were periodically sampled (5 days, 10 days, 30 days, 60 days, 90 days) in a constant temperature heating box at 40 ℃ to perform autoxidation rate measurement, thereby measuring the residual activities and the activity damage ratios of superoxide dismutase in the test samples.
Determination of superoxide dismutase residual Activity in the test sample: under alkaline condition, O can be released by means of rapid autoxidation of pyrogallol 2- The colored intermediate product was produced, and the reaction mixture turned yellow-brown after several minutes and yellow after several hours after the start of the reaction, because of the continuous oxidation of the intermediate product produced. When superoxide dismutase (SOD) exists, the superoxide dismutase can catalyze O 2- And H is + Combining to generate O 2 And H 2 O 2 Thus, accumulation of the intermediate product is prevented, and thus, the enzyme activity of SOD in the sample can be determined by calculation.
The light absorption value at 325nm wavelength was measured by means of an instrumental multi-functional micro-pore detector, and the change in light absorption value per minute within 4min was measured every 30s reading.
Plotting by taking the light absorption value as an ordinate and the time as an abscissa; in the absorbance versus time graph described above, the following formula is calculated:
wherein: Δa325 is the reaction rate of the autoxidation tube;
Δa'325 is the reaction rate of the sample tube;
d is the dilution of the test sample.
Vt is the total volume of the reaction solution in ml;
v is the volume of the added to-be-tested sample, and the unit is ml;
3. test results:
the test results are shown in Table 1.
TABLE 1 stability test data for SOD compositions with blue light resistance
As can be seen from Table 1, the SOD compositions with blue light resistance prepared in examples 1 to 3 of the present invention have higher stability at a high temperature of 40 ℃, and in particular, the SOD compositions with blue light resistance prepared in examples 4 to 5, in which cyclodextrin is added as a coating agent, have better stability. The SOD compositions with blue light resistance prepared in comparative examples 2 to 5 have poor stability, which indicates that the synergistic interaction of the components in the formula of the SOD composition with blue light resistance provided by the invention has the improved stability of the SOD composition. Test example two, fibroblast blue light injury protection test
1. Test object:
SOD compositions having blue light resistance were prepared in example 2, example 4 and comparative example 1.
2. The test method comprises the following steps:
2.1 blue light injury model establishment based on mouse embryo fibroblasts
The cells were grown at 1.5X10 3 Density of individual wells/well was inoculated on 96-well plates, and after incubation for 24h, the wells were incubated with LED-BL (blue light) at wavelength λ=450 nm at 5, 15, 25, 45, 85J/cm 2 The blank group was covered with tin foil to protect from light. Media in 96-well plates was discarded and PBS was added prior to irradiation with LED-BL. Immediately after irradiation, the complete medium was added again and placed in an incubator for cultivation. After 24 hours, cell viability assays were performed using MTT.
2.2 Pre-protection test of SOD composition against blue light damage
This test set 5 test samples, 4 replicate wells per test sample, and at the same time, the test set zeroed wells, model wells, control wells (DMEM medium with 10% fetal bovine serum). The test adopts an MTT method to detect the cell viability, and specifically comprises the following steps:
2.2.1, plate:
taking cells in logarithmic growth phase, digesting with EDTA-containing pancreatin, centrifuging to remove supernatant, and adjusting cell density to 1.5×10 4 After/ml, 100uL per well was plated in 96-well plates.
2.2.2 design configuration of test objects at different concentrations: the SOD composition samples with blue light resistance prepared in the example 2, the example 4 and the comparative example 1 are respectively prepared into test samples with different concentration gradients, wherein the specific concentrations are as follows: 100ug/mL, 50ug/mL, 25ug/mL, 12.5ug/mL, and 6.2ug/mL.
2.2.3 dosing:
the cells were dosed to complete adherence (24 h post inoculation), 100uL per well. After completion of the administration, the mixture was left at 37℃with 5% CO 2 Is provided.
2.2.4 moulding:
cells were incubated for 24h with LED-BL (blue light) at wavelength λ=450 nm at 45J/cm 2 The administration group and the model group are irradiated with the irradiation amount of (2), and the blank control group is covered by tinfoil paper to avoid light. Media in 96-well plates was discarded and PBS was added prior to irradiation with LED-BL. Immediately after irradiation, the complete medium was added again and placed in an incubator for cultivation.
2.2.5 cell Activity test:
after cell culture for 24h, the 96-well plates were washed twice with PBS, 100uL of 0.5mg/mL MTT-DMEM basal medium solution was added to each well, and incubated at 37℃for 3 hours in the absence of light. Then 100uL of DMSO was added to each well, and the wells were shaken under a shaker for 15min to read the OD at 570 nm.
2.2.6 calculation formula:
cell viability= (dosing well OD-zeroed OD)/(control well OD-zeroed OD) ×100%
3. Test results:
3.1 blue light injury results based on mouse embryo fibroblasts: the cells were set at 5, 15, 25, 45, 85J/cm from low to high 2 Total 5 blue light irradiation amounts, the blue light damage result is 45J/cm 2 A damage peak occurs.
3.2, data of pre-protection test of SOD composition against blue light damage are shown in Table 2.
TABLE 2 Pre-protection test data for blue light damage by SOD compositions
As shown in Table 2, the SOD composition with blue light resistance prepared by the invention has better blue light resistance and skin injury repairing effects.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.
Claims (5)
1. The SOD composition with the blue light resistance is characterized by comprising the following components in percentage by mass:
0.01-2% of superoxide dismutase, 0.01-2% of polypeptide, 5-10% of stabilizer, 2-5% of synergist, 10-30% of solubilizer, and adding purified water to 100%;
the polypeptide is one or the combination of more than two of carnosine, glutathione, acetyl tetrapeptide-2 and palmitoyl tetrapeptide-10;
the stabilizer is hydroxyethyl piperazine ethane sulfonic acid and sodium hyaluronate, and trehalose is optionally added;
the synergistic agent is p-hydroxyacetophenone;
the solubilizer is butanediol and pentanediol.
2. The SOD composition with blue light resistance according to claim 1, comprising the following components in percentage by mass:
0.1-2% of superoxide dismutase, 0.1-2% of polypeptide, 5-8% of stabilizer, 2-5% of synergist, 10-28% of solubilizer, and 100% of purified water.
3. The SOD composition with blue light resistance according to claim 1 or 2, wherein the SOD composition with blue light resistance is further added with 5-10% cyclodextrin by mass.
4. A method for preparing the SOD composition with blue light resistance according to any one of claims 1 to 3, comprising the steps of:
s1, adding a synergistic agent into purified water, heating to 80-100 ℃, and stirring at 250-350 rpm until the mixture is clear and transparent to obtain a mixed solution;
s2, cooling the mixed solution prepared in the step S1 to normal temperature, then adding superoxide dismutase, polypeptide, stabilizer and solubilizer, and stirring at 450-550 rpm until the mixed solution is clear and transparent, thus obtaining the superoxide dismutase.
5. The method for preparing a SOD composition with blue light resistance according to claim 4, further comprising step S3, specifically: adding cyclodextrin into purified water to prepare a cyclodextrin saturated solution, adding the mixed solution prepared in the step S2 into the cyclodextrin saturated solution, heating to 60-80 ℃ and stirring for 2-3 h, cooling to room temperature, stirring for 6-8 h at room temperature, standing for 12h at 2-8 ℃, taking out, filtering, taking out crystals, adding purified water and stirring to a clear and transparent state, wherein the feed liquid ratio of the crystals to the purified water is 1 g/6 mL, and obtaining the cyclodextrin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210503117.6A CN114948789B (en) | 2022-05-10 | 2022-05-10 | SOD composition with blue light resisting effect and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210503117.6A CN114948789B (en) | 2022-05-10 | 2022-05-10 | SOD composition with blue light resisting effect and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114948789A CN114948789A (en) | 2022-08-30 |
| CN114948789B true CN114948789B (en) | 2023-07-25 |
Family
ID=82982123
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210503117.6A Active CN114948789B (en) | 2022-05-10 | 2022-05-10 | SOD composition with blue light resisting effect and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114948789B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117357459A (en) * | 2023-11-04 | 2024-01-09 | 河北贵秋绿色生物科技有限公司 | Collagen peptide composition with blue light resisting effect and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109394566A (en) * | 2018-12-19 | 2019-03-01 | 诺斯贝尔化妆品股份有限公司 | A kind of facial mask essence for repairing blue light damage |
| KR20200030191A (en) * | 2018-09-12 | 2020-03-20 | 정철민 | Manufacturing method of cosmetic composition comprising superoxide dismutase capsule |
| CN112972339A (en) * | 2021-04-12 | 2021-06-18 | 东晟源研究院(广州)有限公司 | Blue light prevention spray |
| CN114306124A (en) * | 2021-12-24 | 2022-04-12 | 上海忱扬生物科技有限公司 | Supermolecule preparation with antioxidant, anti-sugar and anti-blue-light effects and preparation method thereof |
-
2022
- 2022-05-10 CN CN202210503117.6A patent/CN114948789B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20200030191A (en) * | 2018-09-12 | 2020-03-20 | 정철민 | Manufacturing method of cosmetic composition comprising superoxide dismutase capsule |
| CN109394566A (en) * | 2018-12-19 | 2019-03-01 | 诺斯贝尔化妆品股份有限公司 | A kind of facial mask essence for repairing blue light damage |
| CN112972339A (en) * | 2021-04-12 | 2021-06-18 | 东晟源研究院(广州)有限公司 | Blue light prevention spray |
| CN114306124A (en) * | 2021-12-24 | 2022-04-12 | 上海忱扬生物科技有限公司 | Supermolecule preparation with antioxidant, anti-sugar and anti-blue-light effects and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114948789A (en) | 2022-08-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106727027B (en) | Blue light resisting, oxidation resisting and free radical scavenging composition, skin care product and preparation method | |
| CN106667794B (en) | Inclusion compound for improving photostability of pigment protein and application thereof | |
| CN114948789B (en) | SOD composition with blue light resisting effect and preparation method thereof | |
| BRPI0717551B1 (en) | “Cosmetic active ingredient, cosmetic composition, use of a sarcodiotheca extract, and cosmetic treatment method” | |
| CN111481465B (en) | Ascorbic acid protective agent and application thereof | |
| CN108392436B (en) | Skin-whitening and anti-aging skin-care composition | |
| CN109316368A (en) | Anti-aging skin care product and preparation method thereof | |
| CN110464672B (en) | Cosmetic composition with safe antioxidant effect | |
| CN111514072B (en) | Millettia speciosa-noni composite enzyme, preparation method thereof and application of millettia speciosa-noni composite enzyme in preparation of facial mask | |
| CN114288216A (en) | Antioxidant and anti-saccharification composition and preparation method thereof | |
| CN112618457A (en) | Chloasma-removing composition and preparation method and application thereof | |
| JPH0581566B2 (en) | ||
| CN114306124A (en) | Supermolecule preparation with antioxidant, anti-sugar and anti-blue-light effects and preparation method thereof | |
| JP2001181173A (en) | Bleaching preparation for external use | |
| JPS63145213A (en) | Prevention of change of color of polyphenol | |
| KR102381645B1 (en) | Cosmetic Composition Comprising Poria cocos Fermentation Extract | |
| JPH1029927A (en) | Antiaging agent | |
| CN110269809B (en) | Skin care nutrient solution prepared from deuterium-depleted water and preparation method thereof | |
| CN113491663A (en) | Superoxide dismutase composition and preparation method thereof | |
| KR102310366B1 (en) | Skin external composition for anti-aging comprising cyanidin-3-o-glucoside and natto gum | |
| CN113244156A (en) | Anti-aging composition and application thereof | |
| JPS6039302B2 (en) | Betacyanin pigment preparation | |
| CN114983923B (en) | A skin external composition capable of simultaneously resisting aging of internal and external sources | |
| TWI838291B (en) | Use of extract of pilea microphylla for manufacturing anti-inflammatory preparations | |
| CN113274304B (en) | High-purity licorice flavone micro-emulsion composition and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |